Abstract
Umbilical cord blood (UCB) cells for allogeneic use are collected and frozen in more than 130 public CB banks worldwide. More than seven hundred and fifty thousands CB units (CBU) are available for transplantation. In this chapter we will describe some procedures for cord blood collection, processing, banking and recommendations on how to choose a single or double UCB unit for transplantation (Garcia 2010).
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1 Introduction
Umbilical cord blood (UCB) cells for allogeneic use are collected and frozen in more than 130 public CB banks worldwide. More than seven hundred and fifty thousands CB units (CBU) are available for transplantation. In this chapter we will describe some procedures for cord blood collection, processing, banking and recommendations on how to choose a single or double UCB unit for transplantation (Garcia 2010).
2 Collection
Donor recruitment usually starts during the antenatal period, with objective information given by woman’s health-care provider. After consent, trained personnel need to determine donor eligibility to ensure that donation is safe for future patients. To assess donor eligibility, a donor medical history interview shall be conducted identifying risk factors for transmissible and genetic disease. In addition, infectious disease markers (IDM) performed to maternal blood samples will be obtained within 7 days before or after the collection of the UCB unit. These samples will be tested for evidence of infection of HIV-1, HIV-2, hepatitis B, hepatitis C, HTLV-I, HTLV-II, syphilis and any additional markers according to local regulations.
Collection must not interfere with normal delivery attention. A successful collection should have a high collection volume and a high total nucleated cell count, be non-contaminated and have the proper documentation. A UCB collection typically involves cord clamping (delayed clamping up to 1–2 min is still compatible with public donations) (Frändberg et al. 2016), disinfection, venipuncturing of umbilical vein and draining by gravity avoiding clotting. Collection bag should be appropriately labelled.
There are two main techniques to collect UCB from the cord vein: before the placenta is delivered (in utero) or after the placenta is delivered (ex utero). Both collection techniques have their own unique advantages and disadvantages, but both techniques require that the individuals performing the collections be adequately trained.
After collection, typically health-care provider will complete a report describing labour and completing variables that could be useful to release the unit like the presence of fever, complications, type of delivery, etc. In case of unexpected adverse reactions during collection they need to be communicated to the competent authority. After collection, it may be required a follow-up of the donor including health questionnaires. Additionally, if any abnormal result is detected during testing, a counseling process should be in place.
UCB units shall be transported to the processing facility, and sometimes, these facilities could be far away from the collection sites. A validated procedure for transportation between these two facilities is needed to demonstrate a reliable method. Standard procedures shall be in place to describe time and temperature of storage and transportation methods. All transportation records shall allow tracking back from the collection site to the UCB bank, and any deviation should be recorded.
3 Processing and Banking
3.1 UCB Cell Processing
Unrelated UCB unit must arrive at the processing laboratory in time to allow initiation of cryopreservation within 48 h of collection (this time is extended to 72 h for related or directed UCB donations). The decision as to whether a collected UCB unit will be acceptable for processing and banking will be made based on the acceptance criteria specified by the UCB bank. Many banks have further refined their acceptance criteria based on economics and the desire to build an international inventory of UCB units with very high TNC or percentage of ethnic minorities. Many UCB banks are now committed to processing and storing only those UCB units with high TNC (e.g. >20 × 107 TNC or higher), based on the greater likelihood of these units being used (Saccardi et al. 2016).
Volume reduction of UCB is considered essential to the provision of a high-quality product and cost-effective UCB banking. Reducing the volume of the final product allows for storage efficiency in terms of space and cost and, most importantly, reduces the risk of ABO incompatibility and DMSO toxicity to the potential recipient. Despite some loss of cells, volume reduction has additional practical and clinical benefits; the process yields RBC and plasma components as waste products that can be used for immediate or future testing, thereby minimizing the loss of the actual stem cell product for testing purposes. Different methods for volume reduction are available (Hough et al. 2016).
The selection of a suitable protocol for cryopreservation of UCB for use in transplantation is critical to optimize the recovery of functionally viable progenitor cells, most of which lie within the CD34+ compartment. Some important considerations that are potential sources of cell damage include the type and concentration of cryoprotectant, the cell concentration and the cooling and warming rates. UCB units must be stored in freezing bags designed and approved for the cryopreservation of human cells and placed into metal canisters to afford protection during freezing, storage, transportation and shipping. It is important that after filling, each freezing bag is visually examined for possible leaking and breakage of seals.
UCB units should be cryopreserved using a controlled rate freezer with a validated freezing programme. Liquid nitrogen-based controlled rate freezers have been used to ensure long-term maintenance. Minimizing transient-warming events is very important for that. Stability programmes should be designed in order to establish the expiration time of the UCB stored.
3.2 Testing and Quality Assessment
Table 18.1 shows release specification for UCB units. Quality assessment is written below:
Safety
It is essential that UCB is screened for those infectious diseases which can be transmitted via blood (as described above). In addition, product should be free of microbial contamination (or with an appropriate antibiogram for related uses). Prior to release for administration, each UCB unit must have undergone hemoglobinopathy screening.
Identity
At least, HLA-A, HLA-B, HLA-C and DRB1 loci must be determined using DNA-based methods and result included when listing a UCB unit on the search registries. It is recommended that HLA typing is performed in an accredited laboratory. ABO blood group and Rh type must be reported prior to listing a UCB unit for search. Prior to release of a UCB unit for administration, it is imperative that HLA identity is confirmed. Ideally, confirmatory typing will be performed on a sample taken from a contiguous segment. HLA typing on maternal blood may also be performed prior to release of a UCB unit. Haplotype matching between maternal donor and infant donor confirms linkage between the two and serves as a secondary confirmation of identity.
Purity
UCB unit specifications report total nucleated cells, total nucleated RBC count and CD34+ cells, and a cell blood count with differential should be performed, with parameters for neutrophils, lymphocytes, monocytes and platelets defined.
Potency
Potency testing to determine the growth potential and viability of progenitor cells in a UCB unit should be performed post-processing (prior to cryopreservation), in addition to being performed on a representative thawed sample prior to release for administration.
4 Selecting CBU for Transplantation
The success of the UCB transplantation (UCBT) will depend on the characteristics of the CBU. Tables 18.2 and 18.3 list the recommendation of choosing single and double cord blood units, respectively, for transplantation.
Key Points
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Cord blood donation comprises the following steps: informative and consent process, revision of eligibility criteria, cord blood collection and finally fresh storage before a standardized transportation to the processing cell lab.
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Cell processing labs require coordination of production and quality control labs to transform the altruistically donated raw material in a medicinal product with predefined specifications that ensure its safety, identity, purity and potency.
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A public cord blood bank is a stem cell registry that provides ready-to-use banked medicinal products for any patient in need through international networking of stem cell donor organizations.
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Cord blood selection is based on sorting CB units using primary criteria (cell content and HLA matching) followed by ranking based in secondary criteria depending on disease status, conditioning, age and recipient’s weight.
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Querol, S., Rocha, V. (2019). Procurement and Management of Cord Blood. In: Carreras, E., Dufour, C., Mohty, M., Kröger, N. (eds) The EBMT Handbook. Springer, Cham. https://doi.org/10.1007/978-3-030-02278-5_18
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