Abstract
The Wolffian ducts (WD) are paired epithelial tubules central to the development of the mammalian genitourinary tract. Outgrowths from the WD known as the ureteric buds (UB) generate the collecting ducts of the kidney. Later during development, the caudal portion of the WD will form the vas deferens, epididymis and seminal vesicle in males, and will degenerate in females. While the genetic pathways controlling the development of the UB are firmly established, less is known about those governing development of WD portions caudal to the UB. Sprouty proteins are inhibitors of receptor tyrosine kinase (RTK) signaling in vivo. We have recently shown that homozygous mutation of a conserved tyrosine (Tyr53) of Spry1 results in UB defects indistinguishable from that of Spry1 null mice. Here, we show that heterozygosity for the Spry1 Y53A allele causes caudal WD developmental defects consisting of ectopically branched seminal vesicles in males and persistent WD in females, without affecting kidney development. Detailed analysis reveals that this phenotype also occurs in Spry1+/– mice but with a much lower penetrance, indicating that removal of tyrosine 53 generates a dominant negative mutation in vivo. Supporting this notion, concomitant deletion of one allele of Spry1 and Spry2 also recapitulates the genital phenotype of Spry1Y53A/+ mice with high penetrance. Mechanistically, we show that unlike the effects of Spry1 in kidney development, these caudal WD defects are independent of Ret signaling, but can be completely rescued by lowering the genetic dosage of Fgf10. In conclusion, mutation of tyrosine 53 of Spry1 generates a dominant negative allele that uncovers fine-tuning of caudal WD development by Sprouty genes.
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Introduction
Development of the genitourinary system of vertebrates begins shortly after gastrulation through the differentiation of the intermediate mesoderm. This embryonic tissue proliferates, and in some cells, mesenchymal–epithelial transition is induced to generate a pair of longitudinal tubules known as the nephric or Wolffian Ducts (WD). Development of the definitive kidney (metanephros) begins on embryonic day (E)10.5 at the caudal end of the WD, where GDNF secreted by the adjacent metanephric mesenchyme prompts the formation of an outgrowth of the WD, the ureteric bud (UB). Subsequently, GDNF, through its receptor tyrosine kinase (RTK) Ret, causes the UB to repeatedly branch and ultimately generate the collecting duct system of the kidney. Later, during development, the WD will also give rise to the epididymis, vas deferens and seminal vesicle (SV) in males but will degenerate in females. While the genetic pathways involved in the development of the UB are well understood, those regulating caudal WD differentiation into male sexual ducts or regression in females are less understood.
The Sprouty family of genes (Spry1–4) encodes feedback inhibitors of RTK activity. In mice, targeted deletion of Spry2 and/or Spry4 causes craniofacial abnormalities due to hypersensitivity to FGF [1, 2]. In addition, genetic deletion of Spry2 leads to hyperplasia of the enteric nervous system as a result of excessive Ret signaling [3], whereas Spry1 antagonizes Ret signaling during kidney morphogenesis [4, 5]. Mice deficient in Sprouty1 grow more than one ureteric bud per side of the embryo, owing to excessive activation of Ret signaling [4, 6, 7].
The mechanisms by which Sprouty family members restrain RTK activity are ill-defined. Several in vitro studies have identified a tyrosine located to the N-terminus of the protein that is critical for regulation of Sprouty activity [8,9,10]. We have recently validated these observations in vivo for the conserved N-terminal tyrosine of Sprouty1, tyrosine 53 [11]. In that work, we show that Spry1 knockin mice lacking tyrosine 53 phenocopy renal defects found in Spry1 knockout animals, consisting of ectopic UB budding and abnormal ureter maturation. In addition, Sprouty mutants lacking their N-terminal tyrosine function as dominant negative alleles in vitro. Whether this behavior also takes place in vivo is currently not known.
While characterizing our knockin mice of Sprouty1 lacking tyrosine 53, we observed the emergence of a novel phenotype affecting caudal WD development. Heterozygous Spry1Y53A/+ females showed septate vaginas flanked by WDs that did not degenerate but persisted into adulthood, while male mutants exhibited ectopically branched SVs. This phenotype was also observed in Spry1+/– mice of similar genetic background but with much lower incidence, suggesting that mutation of tyrosine 53 generated a dominant negative allele. Simultaneous removal of one Spry1 and one Spry2 allele recapitulated the Spry1Y53A/+ phenotype with high penetrance, indicating that Spry1 and Spry2 collaborate in governing caudal WD development. Finally, we provide in vivo mechanistic data demonstrating that these defects were not caused by excessive Ret signaling as removal of both Ret alleles did not mitigate the phenotype. Instead, removal of one Fgf10 allele completely rescued these abnormalities, pointing to unrestrained signaling by FGF10 as the underlying cause of these defects.
Results
Caudal Wolffian duct defects but normal renal development in Spry1Y53A heterozygous mice
We have recently reported that Sprouty1Y53ANeo/Y53ANeo knockin mice phenocopy renal defects found in Spry1 null mice [11]. When backcrossing Spry1Y53A/+ mice into a C57BL/6 genetic background, we noticed a sharp decline in fertility of both male and female Spry1Y53A/+ mice. Examination of Spry1Y53A/+ females in a ~ 90% C57BL/6 background revealed that all of them (n = 12) had imperforated vaginas and swollen perineum (Fig. 1A). Consistently, these females presented hydrometrocolpos, a massively enlarged, fluid-filled uterus occupying most of the abdominal cavity owing to accumulation of uterine gland secretions (Fig. 1B). Histological analysis revealed that vaginas from Spry1Y53A/+ females presented septa that sometimes resulted in completely duplex vagina (Fig. 1C). Moreover, vaginal walls presented paired cystic cavities running parallel to vaginal lumens, lined by monostratified epithelium, similar to WD remnants known as Gartner cysts in humans (Fig. 1C). On the other hand, Spry1Y53A/+ males in the same genetic background presented normal testes, epididymes and vasa deferentia (data not shown), but SVs were duplicated or presented ectopic branches in most animals (Fig. 1D). Microscopic examination showed normal histological organization of the SV (Fig. 1D). Of note, gross morphology of kidneys and ureters appeared normal in these mice (Fig. 1B).
We next performed whole-mount cytokeratin staining at different key embryonic ages to characterize development of the genitourinary system of these mice. No differences in UB budding or branching were observed between wild type and Spry1Y53A/+ animals at E11.5 and E13.5, respectively (Fig. 2A, top and middle panels). Ureter maturation was also normal in Spry1Y53A/+ mice (Fig. 2A, bottom panel, and Supplemental Videos 1 and 2). As we previously described [11], only a small proportion of heterozygous animals displayed single ectopic UBs leading to unilateral duplex ureters that unlike those from Spry1Y53A/Y53A mice correctly separated from the WD (Supplemental Fig. 1A, B). The presence of WD remnants in adult Spry1Y53A/+ females prompted us to investigate whether WD degeneration failed along the whole length of the tubules or just at their caudal-most portion. As shown in Fig. 2B, WD degenerated normally throughout most of their length, except for the portions located at the level of the vagina. These remnants were clearly present at birth in heterozygous animals but absent in wild type littermates (Fig. 2B and Supplemental Video 3). In males, WD maturation appeared roughly normal up to E16.5, but showed ectopic branching at birth (Fig. 2C). Besides incomplete fusion at their caudal tips leading to septate vagina, Müllerian ducts (MD) appeared normal. In conclusion, abnormal caudal WD development appeared in late stages of embryonic development of Spry1Y53A/+ mice, which display largely normal urinary tract development.
Mutation of Spry1 tyrosine 53 generates a dominant negative allele
Previous data demonstrate that overexpressed Sprouty mutants lacking their N-terminal tyrosine act as dominant negative mutants in vitro [8,9,10]. To ascertain if the same was true in vivo, we compared the penetrance of the above caudal WD defects in Spry1Y53A/+ vs. Spry1+/– mice. To control for genetic background effects, we analyzed both genotypes in ~ 90% as well as ~ 40% mixed C57BL/6 × 129 Sv background (see Supplemental Fig. 2 for details on the crosses performed). As shown in Fig. 3A, the caudal WD phenotype was not restricted to Spry1Y53A/+ animals but was also found in Spry1+/– mice. Second, in both Spry1Y53A/+ and Spry1+/– mice, the phenotype was dependent on the genetic background, being more penetrant in purer C57BL/6 backgrounds. Finally, the penetrance of caudal WD abnormalities was always higher in Spry1Y53A/+ than in Spry1+/– mice of similar genetic background, thus meeting the definition of a dominant negative allele.
One of the mechanisms by which loss-of-function mutants behave as dominant negatives is that their expression levels are abnormally high, thus interfering with normal functioning of signaling pathways (for example competing with wild-type molecules for upstream activators). We, therefore, examined Spry1 protein levels in the SV of Spry1Y53A/+ mice vs Spry1+/+ littermates and found much higher levels in the former (Fig. 3B, C). Such an increase was post-transcriptional since mRNA levels were comparable between phenotypes (Fig. 3C). This is in agreement with a robust increase of Spry1 protein (but not mRNA) levels in the developing kidney of Spry1Y53ANeo/Y53ANeo mice [11]. Thus, Spry1 Y53A could exert its dominant negative effects at least partially via elevated protein levels. In situ hybridization using RNAscope revealed that Spry1 mRNA was mostly expressed in the epithelium of the seminal vesicle and WD remnants at birth (Fig. 3D). Likewise, at E15.5, Spry1 expression was prominent in the WD epithelium, with moderate expression levels in the mesenchyme, specially that surrounding the MD (Supplemental Fig. 3B). Again, at this embryonic age mutation of tyrosine 53 did not increase mRNA levels in the WD epithelium (Supplemental Fig. 3B). The specificity of the technique was validated using Spry1 knockout tissues as negative control (Supplemental Fig. 3A). Finally, the pattern of Spry1 expression mirrored that of ERK phosphorylation, in agreement with the previously described expression of Sprouty genes at the sites of growth factor signaling (Supplemental Fig. 3C). However, we did not detect gross differences in ERK phosphorylation between wild type and Spry1Y53A/+ mice, presumably due to the narrow dynamic range of immunohistochemistry (Supplemental Fig. 3C).
Spry1 and Spry2 cooperate to pattern internal genitalia
Spry1 and Spry2 have overlapping functions during development of a variety of structures such as the cerebellum [12], inner ear [13], eyelids [14], teeth [15], or external genitalia [16], among others. To explore whether Spry1 and Spry2 also collaborate in patterning of the caudal WD, we crossed Spry1+/– mice to Spry2+/– mice to obtain double heterozygous animals. Examination of external genitalia of 12 double heterozygous females revealed that ~ 80% of them had imperforated vagina with hydrometrocolpos, and septate vaginas flanked by WD remnants (Fig. 4), despite showing correctly formed kidneys and lower urinary tracts (not shown). Likewise, double heterozygous males presented duplicated SVs in a similar proportion (~ 80%, n = 17; Fig. 4), with no other apparent abnormalities in testis, epididymis and vas deferens. Importantly, the penetrance of such caudal WD defects in single heterozygous mice was negligible, according to its mixed genetic background (50% C57BL/6 × 129 Sv). Thus, removal of one allele each of Spry1 and Spry2 resulted in a large percentage of animals displaying a caudal WD phenotype identical to that observed in Spry1Y53A/+ mice, demonstrating that Spry1 and Spry2 collaborate with each other during development of the caudal WD.
Caudal Wolffian duct defects are Ret-independent
Since Spry1 antagonizes Ret signaling during ureteric bud outgrowth and branching, we wanted to analyze whether upregulated Ret activity underlies these caudal WD defects. We first examined the expression pattern of Ret in the caudal WD during development (E14.5–E16.5), at birth and at postnatal day 7. To do so, we took advantage of knockin mice expressing EGFP from the Ret locus (RetEGFP/+, Fig. 5A) [17]. In males, EGFP fluorescence was localized to the caudal WD from E14.5 to E16.5 and persisted in SVs from birth to at least the first postnatal week (Fig. 5B). In wild-type females, the WD was positive for EGFP fluorescence until it regressed at around E16.5 (Fig. 5C, left panel). We then generated Spry1+/–; Spry2+/–; RetEGFP/+ triple mutant mice and analyzed their caudal WD remnants by EGFP fluorescence at birth. As shown in Fig. 5C (right panel), WD remnants found in newborn mutant females were also positive for Ret.
We then generated Spry1+/–; Spry2+/– mice either expressing Ret (RetEGFP/+) or not (RetEGFP/EGFP) and analyzed their caudal WDs by EGFP fluorescence at birth, since Ret-knockout animals die shortly after birth owing to renal agenesis (Fig. 6). In pups wild type for Spry1 and Spry2 only one SV per side of the embryo was formed, irrespective of the Ret genotype (RetEGFP/+ or RetEGFP/EGFP; Fig. 6A), indicating that Ret is dispensable for patterning of the SV. More importantly, caudal WD defects found in Spry1 and Spry2 double heterozygous mice were unaffected by deletion of Ret in both male and females pups (Fig. 6A, B). Taken together, these observations indicate that caudal WD defects responsible for the internal genitalia abnormalities presented by Sprouty mutant mice are not caused by dysregulated Ret signaling. This is in sharp contrast to what happens with UB development, which is very sensitive to changes in the GDNF-Ret signaling axis.
Caudal WD defects are completely rescued by heterozygous deletion of Ffg10
Since aberrant Ret signaling did not appear to contribute to caudal WD defects, we sought to explore whether dysregulated activity of other RTKs could be responsible for them. To narrow down putative candidates, we performed RNAseq on pooled SVs from wild-type newborn animals and examined RTK expression. As shown in Supplemental Table 1, Fgfr2 and Fgfr1 were among the top 5% most abundant genes in neonatal SV, ranking as the third and fifth most expressed RTKs, respectively. Moreover, FGF7 and FGF10 have been shown to promote branching morphogenesis in organ culture of SVs [18, 19], and WD retention in explanted female metanephroi [20]. We analyzed whether our phenotype could be rescued by attenuating Fgf10 signaling since its expression roughly doubled that of Fgf7 in neonatal SVs (Supplemental Table 2). To do so, we generated either Spry1Y53A/+ or Spry1+/–; Spry2+/– mice lacking one copy of Ffg10. The former were generated by crossing Spry1Y53A/+ mice to Fgf10+/– mice, whereas the latter were produced by mating Spry1f/f; Spry2f/f; UbC-CreERT2 male mice injected with tamoxifen at 4 weeks of age to Fgf10+/– females (therefore, only Spry1+/–; Spry2+/–; Fgf10+/+ or Spry1+/–; Spry2+/–; Fgf10+/– were generated, see Supplementary Fig. 4). As shown in Fig. 7, genetic ablation of a single copy of Fgf10 completely rescued the caudal WD phenotype caused by both heterozygous expression of Spry1Y53A and double heterozygous deletion of Spry1 and Spry2, indicating that loss of function of Spry genes cause the caudal WD phenotype at least in part by exacerbating FGF10-mediated downstream signaling. Finally, we aged Spry1Y53A+/–; Fgf10+/– to adulthood and examined vaginal opening and fertility. All Spry1Y53A+/–; Fgf10+/– female mice (n = 8) displayed correctly perforated vaginal opening. To check fertility, these females were mated to four Spry1Y53A+/–; Fgf10+/– male mice. All females produced litters, indicating that loss of a single Fgf10 allele rescued fertility problems seen in Spry1Y53A/+ mice.
Discussion
In this work, we report a novel function for Sprouty proteins in patterning internal genitalia by controlling caudal WD development. This previously unnoticed role was revealed by the dominant negative nature of Spry1Y53A mutation, which in contrast to the null mutation, causes the emergence of the phenotype with high penetrance. We also report that removing one allele of Spry2 in the context of Spry1 heterozygosity recapitulates this WD phenotype with high penetrance. Unlike renal malformations, these WD abnormalities are independent of Ret signaling but are rescued by decreasing the genetic dosage of Fgf10.
Removal of the N-terminal tyrosine of Sprouty proteins has been shown to generate dominant negative mutants in vitro. Thus, Sasaki et al. [8] reported that expression of either Y53A Spry4 or Y55A Spry2 reverses the inhibitory effect of wild-type Spry2 or Spry4 on ERK activation, respectively. Furthermore, expression of HA-tagged Y55F Spry2 blocks FGF-induced tyrosine phosphorylation of myc-tagged Spry2 or Spry1 in a dose-dependent manner [9]. We have found that Spry1Y53A protein levels are much higher than those of wild type protein in SVs, thus providing a simple mechanism by which the mutant behaves as a dominant negative.
Why are protein levels of Spry1 Y53A higher? Compelling evidence show that binding of c-Cbl to the N-terminal tyrosine of human Spry2 promotes its ubiquitin-dependent degradation [21,22,23]. We speculate that analogously, lack of Tyr53 of Spry1 results on its accumulation owing to defective binding to c-Cbl and compromised proteasomal clearance. Higher steady-state levels of mutant Spry1 protein would lead to dominant negative effects, caused by saturation of upstream regulators and/or downstream effectors by the inactive protein.
We have found that Spry1 and Spry2 collaborate in governing caudal WD development. While overlapping activities of Spry1 and Spry2 have been frequently described, others appear to be specific for either protein. For example, Spry1 specifically controls ureteric bud formation [4], whereas Spry2 regulates enteric nervous system or inner ear development [3, 24]. Since the structure of both proteins is highly similar, and given that Sprouty proteins have been previously shown to hetero-oligomerize [9, 10], one likely explanation for these observations is that expression levels in different tissues rather than distinct mechanisms of action would determine the importance of each member in a given tissue (noteworthy, oligomerization depends on the C-terminal, cysteine-rich domain but not on the N-terminal tyrosine [9, 10]). Based on all the above data, together with the observation that Spry1 behaves as a dosage-sensitive gene [6, 25], we propose a model in which global output of Spry1/2 activity would dictate the fate of WD-derived structures (Fig. 8). In this scenario, one likely mechanism to explain the dominant negative effects of Spry1 Y53A would be the so-called “complex poisoning”, where incorporation of one mutant subunit inactivates the whole homo- or hetero-oligomer. Overexpression of mutant subunits would potentially contribute to make the phenotype more penetrant.
Caudal WD abnormalities were not rescued by removing both alleles of Ret, indicating that unlike UB defects, they were not caused by unrestrained Ret signaling. Instead, removing one single copy of Fgf10 completely prevented their emergence. Our RNAseq data show that Fgfr2 and to a lesser extent Fgfr1 are abundantly expressed in the mouse neonatal SV. In situ hybridization experiments show that Fgfr2 is robustly expressed in the caudal WD epithelium but not its surrounding mesenchyme, whereas Fgfr1 is expressed in the mesonephric tubules and the caudal WD mesenchyme during development [26, 27]. On the other hand, signaling by Fgfr2 supports caudal WD maintenance, as conditional deletion of Fgfr2 in the WD epithelium using HoxB7-Cre transgenic mice results in regression of the caudal, but not cranial WD [27]. Interestingly, the UB appeared to develop normally in these mice. In line with these pieces of evidence, Kuslak and colleagues [26] have demonstrated that the spontaneous mouse SV shape (svs) mutation, which affects budding/branching of the SV is caused by a mutation of the Fgfr2 gene that perturbs splicing of the gene. Authors also show that the alternative splice isoform Fgfr2c, whose cognate ligands are FGF7 and FGF10 [28], is the main isoform expressed in the SV [26]. Previous work has shown that both FGF7 (also known as KGF) and FGF10 are expressed in the mesenchyme surrounding SVs during development and, as mentioned earlier, that both factors can promote SV branching in vitro [18, 19]. Likewise, exogenous FGF7 and/or FGF10 causes retention of the WD in explanted female mesonephroi at different embryonic ages [20]. These data suggest that both FGF7 and FGF10 play a role controlling caudal WD development. However, while the phenotype of FGF7 knockout mice is relatively mild with no reported defects in internal genitalia [29], deletion of FGF10 dramatically affects both prostate and SV development [30]. In light of these data and our own observations, we conclude that FGF10 is the major regulator of caudal WD development, although a redundant role of FGF7 cannot be excluded.
Deletion of one Fgf10 allele also rescued imperforate vagina found in Spry1Y53A/+ mice. Opening of the vaginal orifice in mice occurs at puberty (around 3–4 weeks of age), by canalization of the vulvar epithelium via estrogen-mediated apoptosis. The role of Fgf10/Spry1 in this process could, therefore, be independent of caudal WD defects. For example, mutation of Spry1 could lead to abnormal estrogen production or promote resistance to apoptosis of the vulvar epithelium. It is also possible that persistent WD could indirectly affect vaginal opening, by disturbing proper vaginal development. These possibilities await future investigations.
What are the cellular mechanisms controlling WD regression in females? Two key reports demonstrate that several structures in the developing embryo including the WD in females degenerate via induction of (programmed) cellular senescence [31, 32]. This type of cellular senescence relies on expression of the cdk inhibitor p21, and its transcriptional signature is characterized by increased activation of the TGFβ, Shh and Wnt/β-catenin pathways [31]. Interestingly, adult female mice lacking p21 show septate vagina, although persistent WD was not examined [31]. Interestingly, the phenotype of our Spry mutants is strikingly similar to that of a recently described β-catenin mutant, both in males and females [33]. An important next step will be investigating the relationship between Sprouty proteins, the Wnt/β-catenin pathway and cellular senescence during development of the caudal WD.
Methods
Mice
All animal use was approved by the Animal Care Committee of the University of Lleida in accordance with the national and regional guidelines. Mice were maintained on a 12 h light/dark cycle, and food and water was provided ad libitum. Spry1 Y53A (Spry1tm1.1Mns), Spry1 floxed (Spry1tm1Jdli), and Spry2 floxed (Spry2tm1Mrt) mice have been previously described [4, 11, 24, 34]. Fgf10 null mice were generated [35] by crossing Fgf10 floxed mice (Fgf10tm1.2Sms/J) to CMV-Cre mice [B6.C-Tg(CMV-cre)1Cgn/Jas]. Spry2 knockout mice [Spry2tm1.1Mrt, [24]] were obtained from the Mutant Mouse Resource & Research Center (MMRRC, https://www.mmrrc.org). Ubiquitin C-CreER transgenic mice (Ndor1Tg(UBC−cre/ERT2)1Ejb) were from the Jackson Laboratories (Bar Harbor, ME, USA). Spry1 knockout (Spry1tm1.1Jdli, 4) and Ret EGFP mice (Rettm13.1Jmi,17) were generous gifts from Dr. Albert M. Basson (King’s College, London, UK), and from Dr. Sanjay Jain (Washington University, St Louis, USA), respectively.
Whole mount staining
For EGFP fluorescence, specimens were dissected and directly photographed under a Nikon SMZ18 fluorescence stereoscope coupled to a Nikon DS-Ri2 camera. For whole-mount cytokeratin staining, tissues were fixed overnight at 4ºC in 4% paraformaldehyde, blocked in blocking buffer (4% BSA, 1% Triton X100,100 mM glycine, 0.2% sodium azide in PBS) overnight at 4 ºC with gentle agitation and incubated with a 1:100 dilution of anti-cytokeratin antibody (TROMA-I, DSHB) in blocking buffer for 3–5 days at 4 ºC. In some experiments, retrieval using FLASH reagent 2 was performed before blocking as described [36]. In these experiments, blocking buffer was 10% FBS, 1% BSA and 5% DMSO in PBS containing 0.2% Triton X100. After primary antibody incubation, specimens were washed three times in PBS 1% Triton X100 for 2 h each at room temperature, and incubated with fluorescently labeled secondary antibodies (Jackson Immunoresearch) diluted 1: 100 in blocking buffer overnight at 4ºC. Next day, tissues were washed again three times in PBS 1% Triton X100 for 2 h at room temperature. Tissues were cleared in 1:2 benzyl alcohol:benzyl benzoate (BABB) after being dehydrated through a graded series of 25%, 50%, 75% and 100% methanol in PBS (1 h at room temperature each). Specimens were placed on coverslips and imaged using a Olympus Fluoview FV1000 confocal laser scanning microscope or a Nikon SMZ18 fluorescence stereoscope.
Western blot
Protein extracts from tissues were obtained by mechanical lysis using denaturing lysis buffer (50 mM HEPES, 2% SDS), boiled and sonicated. Protein was electrophoresed on 10% SDS polyacrylamide gels, transferred onto PVDF membrane (Millipore) and blocked using 3% BSA (Sigma) in TBS-Tween (0.1%, TBST) for 1 h at room temperature. Membranes were incubated with primary antibodies overnight at 4 ºC. Signal was detected using Horseradish Peroxidase (HRP)-conjugated secondary antibodies (1:10,000, Jackson ImmunoResearch), followed by a chemiluminescence reaction using Amersham ECL substrate (GE Healthcare). Chemiluminescent signal was detected using the VersaDoc Imaging system Model 4000 (Bio-Rad), and densitometry was performed using its software package (ImageLab, Bio-Rad). Primary antibodies were Anti-Spry1 (Cell Signalling, Cat# 13,013 or Cat# 12,993) and Anti β-actin (Santa Cruz, sc-1616).
RT-qPCR
Total RNA was extracted with TRIZOL reagent (ThermoFisher), by homogenizing snap frozen tissue using a TissueLyser LT device (Qiagen). RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher) as per manufacturer’s instructions. Quantitative RT-PCR (RT-qPCR) reactions were performed by means of the SYBR green method, using either the 2 × Master mix qPCR Low Rox kit (PCR Biosystems). The 2−ΔΔCt method was used, normalizing to actin expression. Reverse transcriptase-minus and blank reactions were included in all experiments. Primers used were as follows: Spry1 Fwd 5´-CTCTGCGGGCTAAGGAGC-3´; Spry1 Rev 5´-ACGCCGGCTGATCTTGC-3´; Actin Fwd 5´-TTCTTTGCAGCTCCTTCGTT-3´; Actin Rev 5´-ATGGAGGGGAATACAGCCC-3´.
In situ hybridization, immunohistochemistry and histology
Specimens were fixed in 4% paraformaldehyde overnight at four degrees, dehydrated and included in paraffin. Microtome sections were dewaxed and rehydrated using a xylene/ethanol gradient. For in situ hybridization, the RNAscope® Intro Pack 2.5 HD Reagent Kit Brown- Mm was used exactly as directed by the manufacturers. Probe used was Mm-Spry1 (Cat # 491,311), which targets sequences within the third exon. This is the only coding exon of mouse Spry1 gene and is completely removed in Spry1 knockout mice. For phospho-ERK immunohistochemistry, samples were subjected to antigen retrieval (95 °C for 20 min in Tris/EDTA buffer, pH 9) using a PTLink apparatus (DAKO). Staining was performed by an Autostainer device (DAKO), using anti-phospho-p44/42 MAPK (D13.14.4E) XP® Rabbit mAb from Cell Signaling Technologies at 1/200 dilution. Automated Hematoxylin–Eosin staining was performed using a Coverstainer device (DAKO).
Availability of data and materials
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We are grateful to Dr. Albert M. Basson (King’s College, London), Dr. Sanjay Jain (Washington University, St Louis) and Dr. Daniel Sanchis (Universitat de Lleida) for sharing valuable reagents. We thank Marta Hereu, for her excellent technical assistance.
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Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This work was supported by grants BFU2017-83646-P (MINECO) and PID2020-114947 GB-I00 (MCIU) (both supported by funds from AEI/FEDER, UE) to ME. MV was supported by a predoctoral fellowship from AGAUR. GA and CA and GA are supported by a fellowship from Universitat de Lleida. SC was supported by a Cofund action from the Marie Curie program of the EU.
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Conceptualization, GA, MV, ME; methodology, GA, MV, CE, JR, SB, ODK, XD, JE, ME; investigation, GA, MV, SC, CA, AM, CE, JR, AY, XD, JR, ME; writing–original draft, ME; writing–review and editing, MV, GA, CE; JR, SB, ODK, XD, JE, ME; visualization, MV, GA, ME; supervision, ME; funding acquisition, ME.
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Altés, G., Vaquero, M., Cuesta, S. et al. A dominant negative mutation uncovers cooperative control of caudal Wolffian duct development by Sprouty genes. Cell. Mol. Life Sci. 79, 514 (2022). https://doi.org/10.1007/s00018-022-04546-1
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DOI: https://doi.org/10.1007/s00018-022-04546-1