Abstract
Members of the family Prevotellaceae are Gram-negative, obligate anaerobic bacteria found in animal and human microbiota. In Prevotella bryantii, the Na+-translocating NADH:quinone oxidoreductase (NQR) and quinol:fumarate reductase (QFR) interact using menaquinone as electron carrier, catalyzing NADH:fumarate oxidoreduction. P. bryantii NQR establishes a sodium-motive force, whereas P. bryantii QFR does not contribute to membrane energization. To elucidate the possible mode of function, we present 3D structural models of NQR and QFR from P. bryantii to predict cofactor-binding sites, electron transfer routes and interaction with substrates. Molecular docking reveals the proposed mode of menaquinone binding to the quinone site of subunit NqrB of P. bryantii NQR. A comparison of the 3D model of P. bryantii QFR with experimentally determined structures suggests alternative pathways for transmembrane proton transport in this type of QFR. Our findings are relevant for NADH-dependent succinate formation in anaerobic bacteria which operate both NQR and QFR.
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Introduction
In the rumen of cattle and sheep, a diverse set of microorganisms provides enzymes to degrade ligno-cellulose material and non-proteinaceous nitrogen (Newbold and Ramos-Morales 2020). In 2015, Henderson and co-workers analyzed 742 ruminal samples from 32 animal species from 35 countries and found a “core microbiota” mainly composed of Prevotellaceae, Butyrivibrio and Ruminococcus species (Henderson et al. 2015). They demonstrated that Prevotellaceae, especially Prevotella ruminicola, Prevotella brevis and Prevotella bryantii represent 50–70% of the rumen bacterial population (Gylswyk 1990; White et al. 2014). The genus Prevotella has been recently reclassified into seven genera, and P. bryantii has been renamed Segatella bryantii (Hitch et al. 2022). We are using the traditional nomenclature here, since the reclassification is not translated in the public repositories. With an extensive repertoire of glycoside hydrolases, these bacteria are able to utilize starches, other non-cellulosic polysaccharides and simple sugars as energy sources (White et al. 2014). As an inhabitant of the anoxic ruminal environment, P. bryantii conserves energy in the absence of oxygen. Like other Prevotellaceae, P. bryantii was considered to exclusively perform fermentation (Bryant et al. 1958). During glucose degradation by P. copri (Franke and Deppenmeier 2018), 1 mol glucose is converted together with 0.25 mol CO2 to 1 mol succinate, 1 mol acetate and 0.24 mol formate. Growth is dependent on CO2, indicating that carboxylation of 2-phosphoenolpyruvate (PEP) to oxaloacetate, subsequent formation of fumarate, and reduction of fumarate by quinol:fumarate reductase (QFR) represents the major pathway (Franke and Deppenmeier 2018). This pathway is even more pronounced in CO2-dependent degradation of glucose by P. bryantii (Schleicher et al. 2021a), with a ratio succinate:acetate = 1:0.5. Likewise, utilization of glucose by the human pathogen P. bivia requires CO2 and leads to formation of succinate, malate, and acetate in a ratio 1:0.8:0.5. Interestingly, excreted acetate is consumed by P. bivia at a later growth stage (Schleicher et al. 2021b).
In a metaproteome study from rumen, Deusch and co-workers identified NQR and QFR from different Prevotellaceae and showed that P. bryantii NQR acts as sodium-stimulated NADH:quinone oxidoreductase (Deusch et al. 2019). Both NQR and QFR were enriched from membranes and were shown to operate in a respiratory chain, which couples the oxidation of NADH and reduction of fumarate to succinate to the build-up of an electrochemical sodium gradient (Schleicher et al. 2021a). This pointed to an additional mode of energy generation in P. bryantii reminiscent to anaerobic respiration, which is catalyzed by NQR and QFR. Likewise, the human pathogen P. bivia, which is a causative agent of bacterial vaginosis operates both NQR and QFR and generates a sodium-motive force when grown anoxically on glucose (Schleicher et al. 2021b). In both organisms, menaquinone (MK) was shown to act as electron carrier between NQR and QFR. MK is a low-potential isoprenoid quinone belonging to the subclass of naphthoquinones, characterized by its benzene ring conjugated to the 1,4-benzoquinone structure. MKs exhibit a rather low redox potential (around − 0.07 V) (Magalon and Alberge 2016) and are frequently found in obligate and facultative anaerobic microorganisms. Redox reactions with MK are known for, e.g., hydrogenases, several different NADH dehydrogenases, nitrate reductases, nitrite reductases and fumarate reductases (Simon et al. 2008). Anaerobic respiration utilizing MK was studied in Wolinella succinogenes, a rumen bacterium, which relies on QFR, formate dehydrogenase, Ni/Fe hydrogenase, periplasmic nitrate reductase (Nap) or cytochrome c nitrite reductase (Nrf) for energy conservation, but does not contain NQR or any other redox Na+ pump (Kröger et al. 2002). Accordingly, electron transfer in W. succinogenes is coupled to the generation of a proton motive force (PMF) (Kröger et al. 2002). While SQRs/QFRs are present in the three domains of life, NQR is found exclusively in the bacterial kingdom (Reyes-Prieto et al. 2014). NQR evolved from an ancestral ferredoxin:NAD+ oxidoreductase (FNO) complex in the Chlorobi/Bacteroidetes group and is only limited distributed across the bacterial lineages (Reyes-Prieto et al. 2014). FNO is a membrane-bound electron transfer complex first identified in Rhodobacter capsulatus which was named Rhodobacter Nitrogen Fixation (RNF) complex (Schmehl et al. 1993). FNO/RNF catalyzes the oxidation of ferredoxin and reduction of NAD+ generating an electrochemical sodium (or proton) gradient. The complex may also catalyze the reverse reaction, utilizing electrochemical gradients to drive the endergonic reduction of low-potential ferredoxin with NADH. RNF comprises six subunits (RnfABCDEG), of which RnfACDEG are homologous to the NQR subunits NqrEABDC (Buckel 2021; Boiangiu et al. 2005; Biegel et al. 2011; Vitt et al. 2022).
In this study, we explore the mode of function of NQR and QFR from P. bryantii using homology modeling and in silico docking. We gain insight into the possible mode of binding of MK to the membrane-bound NqrB subunit of Pb-NQR. A comparative analysis of the modeled Pb-QFR structure with experimentally determined structures of type B QFRs leads to a critical assessment of the E-pathway for transmembrane proton transport. Both NQR and QFR are present in selected organisms from different bacterial phyla, suggesting that sodium-motive NADH:fumarate oxidoreduction is not restricted to the Prevotellaceae family.
Material and methods
Protein homology modeling
Structural models of the P. bryantii NQR (Pb-NQR) and QFR (Pb-QFR) were generated based on the 3D structures of the highly related NQR from Vibrio cholerae (PDB 8A1W, PDB 8ACY) (Hau et al. 2023) and QFR from Desulfovibrio gigas (PDB 5XMJ) (Guan et al. 2018). Amino acid sequences of Pb-NQR and Pb-QFR subunits (queries) were retrieved from Uniprot. Structural models of Pb-QFR were generated using Phyre2 (Kelley et al. 2015) and AlphaFold (Jumper et al. 2021), and the resulting models were compared. Subunits of the protein complexes were modeled individually and were superimposed on the template structures. AlphaFold performs structure prediction utilizing neural networks trained by the evolutionary and physical constraints derived from known protein structures. Phyre2 uses an experimentally determined structure of a homolog as a template to predict the structure by performing Hidden-Markov-model (HMM)–HMM matching. This allows for modeling of a protein in the conformation of the respective template. Pb-QFR was modeled in the “normal mode” using a structural template available on the PHYRE2 protein fold recognition server. For modeling of Pb-NQR, “One-to-One threading” was performed using structures of Vc-NQR in the two distinct conformations from the Protein Data Bank (PDB 8A1W, PDB 8ACY). The structural models generated by Phyre2 were compared to models generated by AlphaFold. Note that the Pb-NQR model generated by AlphaFold represents a conformation as observed in the X-ray structure of Vc-NQR (8ACY). The consecutive steps in modeling of protein structures are described in the supplementary material.
Docking of menaquinone into predicted structure of Pb-NQR
Pb-NQR subunit B was modeled with Phyre2 on the basis of the Vc-NQR structure PDB 8A1W with bound ubiquinone-1. This Pb-NqrB model was used for docking calculations. To avoid blocking of the binding site, rotamers of Glu131 and Tyr133 were aligned to the rotamers of the corresponding amino acids in the V. cholerae NQR cryo-EM structure (PDB 8A1W) using COOT 0.9.8.8. Docking was performed using OEDOCKING 4.1.1.0. OpenEye, Cadence Molecular Sciences, Inc., Santa Fe, NM (http://www.eyesopen.com). Pb-NQR structure was prepared for docking by applying a 13 × 23 × 16 Å box over the quinone binding site and creating a contour of the binding site (4940 Å3) using the Make Receptor GUI with balanced settings. Menaquinone-1 was prepared for docking from SMILES using OMEGA 4.1.2.0 (Hawkins et al. 2010). Menaquinone-1 was docked into Pb-NQR with FRED (McGann 2011, 2012).
Identification of NQR, RNF and QFR
Representative organisms from selected bacterial phyla were analyzed, which contained succinate:quinone reductases and/or quinol:fumarate reductases as demonstrated by experimental data. We also searched for NQR and RNF by aligning the amino acid sequences of the NQR subunits from Vibrio cholerae serotype O1 (strain ATCC 39541) and of RnfB from Escherichia coli K12 to the corresponding genomes using the basic local alignment search tool BLAST (Mount 2007). Sequences of subunits of enzymes were retrieved from Uniprot (https://www.uniprot.org/).
Results and discussion
To gain knowledge on the possible functions of P. bryantii NQR and QFR, structural models were obtained by comparisons with the NQR from Vibrio cholerae (Steuber et al. 2014; Hau et al. 2023), and QFRs from W. succinogenes (Madej et al. 2006) and Desulfovibrio gigas (Guan et al. 2018).
3D structural model of QFR from P. bryantii
The model of the P. bryantii QFR (Pb-QFR) was built based on the structure of the Desulfovibrio gigas QFR (Dg-QFR) (Guan et al. 2018). This model was aligned with the QFR of W. succinogenes (Ws-QFR). The Ws-QFR is a type B quinol:fumarate reductase, oxidizing MKH2 and reducing fumarate to succinate. It is composed of three subunits (FrdABC), with FrdA (~ 75 kDa) containing one FAD, FrdB (~ 27 kDa) harboring three FeS centers and FrdC (~ 30 kDa) comprising two hemes b (Lancaster et al. 1999). Ws-QFR forms a homodimer in crystals with a dimension of 75 Å × 120 Å × 50 Å (Lancaster et al. 1999). The catalytic site for MKH2 oxidation, and therefore the entry site of the electrons into the protein, is located in FrdC. After oxidation of MKH2 by the hemes b in FrdC, the electrons are transferred via the proximal heme b and the FeS clusters of FrdB to the covalently bound FAD, and finally, fumarate is reduced to succinate (Lancaster et al. 1999). The 3D crystallographic structure of the Dg-QFR served as template for model building of Pb-QFR by Phyre2 (Kelley et al. 2015). Here, a high confidence, coverage and TM score were calculated, despite moderate overall sequence identities (supplementary material table S3). Next, a more detailed analysis of the secondary structure quality of the modeled QFR structure was performed. The ProQ2 assessment tool implemented in Phyre2 indicates a good quality of the core structures, illustrated by yellow and orange colored cores of the modeled protein structures (Fig. 1, first row). Blue color indicates regions where the quality of the model is lower, which is frequently observed in loops or other connecting protein domains. The clash analysis indicates that only a few domains in the models exhibit unfavorable positions of amino acid side chains. Thus, an overall correct positioning of side chains in the models is assumed (Fig. 1, second row). Furthermore, only a few domains reveal incorrect modeling in terms of rotamer analysis (Fig. 1, third row). Ramachandran analysis reveals only a few disallowed regions near or in the loop domains in the modeled FrdABC structures (Fig. 1, fourth row). The models obtained by Phyre2 and AlphaFold were virtually identical. In summary, the overall stereochemistry and geometry of the structural models of Pb-QFR subunits are adequate, with the exception of few loop regions, which exhibit poor alignment confidence (Fig. 1, fifth row). The model of the Pb-QFR complex was built based on the models of the three Frd subunits (Fig. 2). This model includes FrdA (red), FrdB (green) and FrdC (blue), and six cofactors known from the D. gigas enzyme, which were placed at corresponding positions into the Pb-QFR structural model. Hypothetically, FrdA contains one covalently bound FAD, FrdB comprises three FeS clusters and FrdC harbors two hemes b. Since the W. succinogenes QFR was characterized in terms of both structure and function (Lancaster et al. 2000; Lancaster 2002), the Pb-QFR structural model was compared with the crystallographic structure of the Ws-QFR (PDB 2BS2) to search for conserved amino acid residues, which are likely to be important for substrate conversion, electron and proton transfer.
Predicted mode of operation of QFR from P. bryantii based on the structural model
The binding site for MK in Ws-QFR is suggested to be near the distal heme in FrdC, with Glu66 playing an essential role for MKH2 oxidation (Lancaster et al. 2000) (Fig. 3A). In the modeled Pb-FrdC, a glutamate is predicted in this region (Glu47) but at a position shifted by three amino acid residues (Fig. 3A). Still, the corresponding Glu47 from Pb-FrdC is predicted to be located in a cavity leading to the periplasm, as observed with Glu66 in Ws-FrdC. Glu47 is predicted in a putative loop region of Pb-FrdC, which exhibits only low sequence similarity to the corresponding region in the FrdC from D. gigas, according to the Phyre2 qualification analysis of the modeled Pb-FrdC structure (Fig. 1). In W. succinogenes FrdC, Glu66 accepts protons from quinol and releases them to the periplasm (Lancaster et al. 2000), and a similar function seems likely for Pb-Glu47. Electrons from MKH2 are transferred to the distal heme b, which is coordinated by His44 and His143 in Ws-FrdC (Fig. 3B) (Lancaster et al. 1999). A second, proximal heme b in Ws-FrdC enables electron transfer from the FrdC to the FrdB subunit, facilitated by its location toward the cytoplasmic surface of the membrane and thus to the iron-sulfur clusters of Ws-FrdB. This proximal heme b is also coordinated by two histidines (His93, His182) (Fig. 3C) (Lancaster et al. 1999). The modeled Pb-FrdC structure comprises five transmembrane helices, containing these four histidines in the modeled core structure at the very same positions with the same orientations (His28 and His119 for distal heme b; His69 and His172 for proximal heme b) (Fig. 3B and C).
The [3Fe–4S] cluster, proximal to heme b, is coordinated by the thiol groups of the three cysteines Cys161, Cys208 and Cys214 (Fig. 4B). These amino acid residues are located in a domain, which is in contact with the FrdC subunit. The [3Fe–4S] cluster accepts electrons from the proximal heme b and passes them to the [4Fe–4S], which is ligated by four cysteine residues (Cys151, Cys154, Cys157, Cys218) (Fig. 4C). Electron transfer proceeds via [2Fe–2S] cluster, located between the [4Fe–4S] cluster and the Ws-FrdA subunit. The [2Fe–2S] cluster is coordinated by four cysteine residues (Cys57, Cys62, Cys65, Cys77) (Fig. 4D). In the predicted model of Pb-FrdB, all cysteine residues required for the coordination of the three FeS centers are present and oriented in a manner, which would permit Fe ligation by the thiol groups (Fig. 4). Thus, it can be predicted that Cys174, Cys219 and Cys225 are responsible for [3Fe–4S] coordination (Fig. 4B), and that Cys164, Cys167, Cys170 and Cys229 ligate the [4Fe–4S] (Fig. 4C). The [2Fe–2S] cluster is predicted to be coordinated by Cys62, Cys67, Cys70 and Cys89 (Fig. 4D).
From Ws-FrdB, electrons are transferred to a covalently bound FAD in Ws-FrdA. This FAD is linked by its 8α-methyl group to the histidine His43 (Fig. 5A) (Lancaster et al. 1999). The modeled Pb-FrdB structure predicts with a high confidence also a histidine (His73) at the same position with the same orientation. The Ws-FrdA subunit also comprises a dicarboxylate-binding site for binding of fumarate. This binding site is formed mainly by the isoalloxazine ring of the FAD and four crucial amino acid residues (Phe141, Arg301, His369, Arg404) (Fig. 5B) (Lancaster et al. 1999). Analyzing the modeled Pb-FrdA structure, these four residues are also predicted (Phe148, Arg338, His420, Arg454) in a region with a high prediction quality (Fig. 5B).
The model of Pb-QFR is in accordance with experimental data (Schleicher et al. 2021a) confirming the existence of a P. bryantii QFR composed of three subunits (FrdABC), with similar catalytic functions as described for W. succinogenes (Lancaster et al. 2000, 2005; Lancaster 2013). Although some domains of the Pb-QFR model are predicted at moderate confidence (Fig. 1), the core regions of the model structure responsible for binding of cofactors and substrates are predicted with high confidence and display with a high degree of conservation among QFRs from D. gigas and W. succinogenes. This supports the assumption that P. bryantii operates a QFR belonging to the type B of SQRs. The Pb-QFR model substantiates the biochemical studies (Schleicher et al. 2021a) and provides a good starting point for further mechanistic studies on Pb-QFR.
In fumarate respiration, two protons are liberated by oxidation of MKH2 and two protons are consumed by reduction of fumarate to succinate. The overall reaction contributes to the build-up of an electrochemical proton gradient (H+/e− = 1), if protons from MKH2 are released at the periplasmic aspect of the membrane. In contrast, if protons liberated during oxidation of MKH2 are released at the cytoplasmic side, QFR would operate in an electroneutral manner. For QFR of W. succinogenes, it was shown that protons are released into the periplasm, with an essential role for Glu66 acting as proton acceptor during MKH2 oxidation. Glu66 is located in a cavity connected to the periplasmic aqueous phase (Lancaster et al. 2005). Nevertheless, it was demonstrated that in W. succinogenes, the QFR reaction is electroneutral (Kröger et al. 2002). In a counterbalanced mechanism of H+ movement, two protons are transferred from the periplasm into the cytoplasm via the so-called E-pathway in concert with the two electrons passing from MKH2 via the two heme groups to fumarate (Fig. 6A) (Kröger et al. 2002). For W. succinogenes QFR, it was shown that Glu180 plays a crucial role in this transmembrane proton transport through the E-pathway (Madej et al. 2006). In Pb-QFR, protons from MKH2 oxidation are likely to be released into the periplasm, considering the 3D structural model of Pb-QFR. However, the predicted structure of Pb-FrdC does not confirm a similar position of a Glu at the corresponding position. Thus, an obvious H+ acceptor/donor as identified in the E-pathway of Ws-FrdC is absent in Pb-FrdC (Fig. 6B). Notably, highly similar FrdC subunits from at least four Prevotellaceae species also lack a protonatable amino acid residue at the corresponding position (supplementary material fig. S1), indicating that the E-pathway proposed for W. succinogenes QFR is not strictly retained in QFRs from Prevotellaceae. A tyrosine (Tyr42) near the distal heme in Pb-FrdC is reminiscent to Tyr63 observed in the 3D structure of Desulfovibrio gigas FrdC (Guan et al. 2018). QFR of D. gigas is predicted to facilitate compensatory proton transport, with Tyr63 proposed to act as initial proton acceptor (Guan et al. 2018). In Ws-FrdC, the carboxylic group of the propionate side chain of ring C from the distal heme is considered to act as proton donor/acceptor during proton transport via the E-pathway (Lancaster et al. 2000). In the modeled Pb-FrdC structure, the propionate side chain of the distal heme assumes a very similar position (Fig. 3). NADH:fumarate oxidoreduction in P. bryantii results in the build-up of a sodium-motive, but not of a proton-motive force (Schleicher et al. 2021a). The 3D structural model supports the notion that fumarate reduction by Pb-QFR does not result in the build-up of a proton gradient due to counter-transport of protons, but the identification of the proton pathway requires further investigations.
3D structural model of Pb-NQR
P. bryantii critically depends on primary Na+ transport to energize the membrane, which is catalyzed by a sodium-translocating NADH:quinone oxidoreductase (NQR) (Schleicher et al. 2021a). The structure of Na+-translocating NQR from Vibrio cholerae has been studied intensively and was used to build a reliable structural model of Pb-NQR. Vc-NQR comprises six subunits (NqrABCDEF) and harbors six cofactors: one FAD in NqrF, two [2Fe–2S] clusters in NqrF and between NqrD/E, one riboflavin in NqrB, and two covalently bound FMNs in NqrB and NqrC (Hau et al. 2023; Kishikawa et al. 2022). The subunit NqrF represents the entry site of the electrons from NADH into the complex, thus catalyzing the NADH dehydrogenase reaction. NqrF is composed of two hydrophilic domains, the N-terminal [2Fe–2S] ferredoxin-like domain and the C-terminal FAD-binding domain, and it is anchored into the NQR complex by one single transmembrane helix. NADH is oxidized by FAD and the electrons are transferred to the [2Fe–2S] in the ferredoxin-like domain (Hau et al. 2023; Steuber et al. 2014; Türk et al. 2004). FAD transiently forms a neutral flavosemiquinone during the electron transfer to [2Fe–2S] that serves as a one-electron acceptor. Subsequently, the electrons are transferred from the NqrF ferredoxin-like domain to an electron transfer chain comprising an intramembranous [2Fe–2S] cluster located in the membrane subunits NqrD and NqrE, an FMN in periplasmic subunit NqrC, and FMN and riboflavin in subunit NqrB. In a last step, electrons are transferred from riboflavin to substrate UQ-8 in NqrB, resulting in formation of UQH2. In Pb-NQR, MK acts as ultimate electron acceptor (Schleicher et al. 2021a).
The membrane-bound Vc-NQR complex has dimensions of about 90 Å × 140 Å × 52 Å and an overall molecular mass of 220 kDa (NqrA: 48.6 kDa, NqrB: 45.3 kDa, NqrC: 27.6 kDa, NqrD: 22.8 kDa, NqrE: 21.4 kDa and NqrF: 45.1 kDa). Vc-NQR adopts distinct conformational states, which are crucial for the redox-driven Na+ translocation (Hau et al. 2023). To gain insight into the mode of operation of Pb-NQR, we created models of two states of the catalytic cycle as observed in Vc-NQR (PDB 8ACY and 8A1W) (Hau et al. 2023). Creating such specific models requires a template-based homology modeling approach like in Phyre2, since AlphaFold creates a single model based on all available structures. The models of Pb-NQR created with Phyre2 were highly similar to the corresponding structures of Vc-NQR displaying RMSDs for the Cα atoms of 0.02–0.305 Å for one template (PDB 8ACY) and 0.01–0.26 Å for the second template (PDB 8A1W), respectively. AlphaFold created a model of Pb-NQR that reflects only one conformation. Overall, one conformational model created with Phyre2 and the model created by AlphaFold were highly similar. Subtle differences between both models were observed in subunit Pb-NqrE. Pb-NqrE exhibits an extension of 10 residues compared to Vc-NqE, which are modeled by AlphaFold as an extended periplasmic loop (residues 133–146), while the model of Pb-NqrE places this region into the transmembrane region, which could result in sterical clashes with subunit NqrD. Evaluation of both models and pairwise-alignment favor the model created by AlphaFold. Further minor negligible differences between both models were observed for some solvent-exposed loops, which are not relevant for binding of cofactors or substrates and are not involved in conformational changes.
Figure 7 depicts the two conformations modeled for Pb-NQR. Each model of Pb-NQR comprises all six subunits NqrA-F and all cofactors: NqrF comprises one FAD and one [2Fe–2S] cluster, NqrD/E contain one [2Fe–2S], NqrC harbors one FMN, and NqrB comprises one FMN and one riboflavin. The structures of Vc-NQR (Hau et al. 2023; Steuber et al. 2014) reveal that NQR undergoes drastic structural changes during the catalytic cycle involving at least subunits NqrF, NqrC, NqrD and NqrE. The two distinct structural models of Pb-NQR reflect two different states of the Pb-NQR and support that Pb-NQR also functions as a conformationally driven redox pump (Fig. 7).
Redox cofactor-binding sites in Pb-NQR
The cofactors in Vc-NQR define an electron transfer pathway that passes two times the cytoplasmic membrane (Hau et al. 2023; Steuber et al. 2014). Thus, information on the presence and position of the cofactors are important to evaluate whether such an electron transfer pathway occurs also in Pb-NQR. However, most modeling software suites do not place automatically the corresponding cofactors found in related proteins. Therefore, the cofactors in Pb-NQR were placed by manual model building after critical assessment of the residues in the cofactor-binding sites. The redox cofactor NADH is oxidized in subunit NqrF by the cofactor FAD (Steuber et al. 1997; Türk et al. 2004). The high-resolution structure of Vc-NqrF revealed the binding of NADH (Hau et al. 2023) and a critical role of the C-terminal residue Phe406 in NAD+ release. Pb-NqrF exhibits reasonable sequence similarity to Vc-NqrF; nevertheless, important features are highly conserved in Pb-NqrF and the 3D models show a conserved binding site for cofactor FAD and suggest a highly similar binding mode for NADH (Fig. 8A). In particular, all residues in the active site of the Pb-NqrF model display very similar positions compared to Vc-NqrF. In Vc-NqrF, three residues are in close proximity of the isoalloxazine moiety of FAD: Tyr212, Ser213 and Phe406 (Hau et al. 2023) (Fig. 8B). In the models of Pb-NqrF, the corresponding amino acid residues Tyr216, Ser217, Phe421 are at identical positions and support that FAD and NADH bind as observed in Vc-NqrF (Fig. 8B). In the N-terminal ferredoxin-like domain of Vc-NqrF, the [2Fe–2S] cluster is coordinated by the four cysteine residues Cys70, Cys76, Cys79 and Cys111 (Hau et al. 2023) (Fig. 8C), which are strictly conserved in Pb-NqrF. The 3D model showed that the corresponding residues Cys69, Cys75, Cys78, Cys110 reside in very similar positions and can accommodate an [2Fe–2S] center.
In the integral membrane subunits Vc-NqrD and Vc-NqrE, an [2Fe–2S] cluster is coordinated by cysteine residues originating from both subunits (Cys29 and Cys112 from NqrD; Cys26 and Cys120 from NqrE) (Fig. 9A). This [2Fe–2S] is crucial for electron transfer from NqrF to NqrC across the membrane and is proposed to drive the conformational changes in Vc-NQR (Hau et al. 2023). The 3D model of Pb-NqrD and Pb-NqrE structure confirms that in Pb-NQR, the conserved corresponding four Cys residues (Cys30 and Cys113 from NqrD and Cys26 and Cys120 from NqrE) can coordinate as well a [2Fe–2S] cluster in a highly similar manner (Fig. 9B and C).
From this membrane-bond [2Fe–2S] cluster, electrons are transferred to covalently bound FMN in NqrC. In Vc-NqrC, FMN is covalently attached to Thr225 via a phosphodiester bond (Fig. 10A) (Barquera et al. 2001; Casutt et al. 2012). In Pb-NqrC, the corresponding conserved threonine (Thr185) resides in a similar position (Fig. 10A) putting forward that FMN is covalently attached to this residue. From NqrC, the electron transfer proceeds to subunit NqrB. Like in NqrC, an FMN is covalently attached to a threonine residue. In Vc-NqrB, FMN is bound via a phosphodiester bond to threonine 236 (Fig. 10B) (Barquera et al. 2001; Casutt et al. 2012). The corresponding conserved Thr221 in Pb-NqrB is predicted in a similar position in the 3D model and could bind as well an FMN (Fig. 10B). Thus, the 3D models strongly support the previous finding that Pb-NqrC and Pb-NqrB contain covalently linked flavins (Schleicher et al. 2021a).
In Vc-NQR, electron transport from FMN in NqrB to quinone proceeds via riboflavin (Barquera et al. 2002; Juárez et al. 2008; Tao et al. 2008). Recently published 3D structures of Vc-NQR revealed the position of the riboflavin in Vc-NqrB (Kishikawa et al. 2022; Hau et al. 2023), which forms several hydrogen bonds to protein residues. The amino acid residues Gly198, Asn200, Thr162 and Asp346 form hydrogen bonds with the ribityl chain, and Ala205, Phe342, Thr162 and Asn203 interact with the isoalloxazine structure (Fig. 10C). In the predicted model of Pb-NqrB, the corresponding residues Gly172, Asn174, Thr136, Ala179, Asn177, Phe313 and Asp317 reside in very similar positions forming a riboflavin-binding site (Fig. 10C).
Quinone reduction and Na+ translocation in NqrB of Pb-NQR
From the riboflavin in NqrB, electrons are transferred further to quinone, which serves as an electron shuttle between enzymes of the respiratory chain. The structures of Vc-NQR in complex with UQ-1 or UQ-2 provided first insights into the binding site of UQ in subunit NqrB (Hau et al. 2023). At a rim of NqrB, UQ-1/UQ-2 binds with its head group close to the cytoplasmic aspect of the membrane (Fig. 11A). The interaction of UQ-1/UQ-2 with NqrB is promoted by two amphiphilic helices at the N-terminus of NqrB. The residues Leu26, Leu33, Phe137, Val145 and Phe160 form a hydrophobic pocket for UQ. Furthermore, the carboxyl of the headgroup of UQ forms a hydrogen bond to Asn156. In the model of Pb-NqrB, also two N-terminal amphiphilic helices are predicted (Fig. 11B) and a hydrophobic pocket is formed by the amino acid residues Phe31, Phe34, Tyr111, Phe119 and Leu134 (Fig. 11B). This predicted quinone site in Pb-NQR differs from the site in Vc-NqrB. This difference reflects that Pb-NQR utilizes menaquinone instead of ubiquinone, as P. bryantii exclusively relies on menaquinone for respiration (Schleicher et al. 2021a). To characterize the putative mode of menaquinone binding in Pb-NQR, we performed docking calculations. The results of the docking calculations revealed a binding mode for menaquinone-1, which deviates significantly from UQ binding in Vc-NqrB. In the structure of Vc-NqrB, the headgroup of UQ-2 is tilted by about 45° with respect to the transmembrane helices and the isoprenoid tail of UQ-2 is in van-der-Waals with the proximate transmembrane helix of NqrB. Important for this interaction in Vc-NqrB is residue Gly141 in the transmembrane helix. Gly lacks a sidechain and therefore allows the close interaction of the isoprenoid tail. In contrast, in Pb-NqrB, Leu115 with a bulky sidechain resides in this position. The results of the docking calculations for menaquinone-1 suggest that the naphthoquinone and the isoprenoid moiety of menaquinone-1 interact differently with Pb-NqrB. The naphthoquinone ring is oriented parallel to the transmembrane helices and the isoprenoid moiety exhibits a different orientation. Thus, the 3D model of Pb-NqrB provides some details for the different quinone specificity of NQR from both species.
In Vc-NQR, NqrB exhibits a Na+ translocating pathway that is lined by the backbone carbonyls of residues Val161, Ile164, Leu168 and a constriction formed by the sidechains of Phe338 and Phe342 (Steuber et al. 2014; Hau et al. 2023). In Pb-NqrB, the corresponding residues are strictly conserved and residues Val135, Ile138, Leu142, Phe309, Phe313 reside at the same positions as observed in Vc-NqrB (Steuber et al. 2014; Hau et al. 2023). This strongly suggests that the pathway for Na+ translocation in Pb-NQR is highly similar to Vc-NQR.
Occurrence of QFR and NQR in representatives from bacterial phyla
Succinate:quinone reductases (SQR) are divided into the four types A–D based on their architecture (number of subunits and heme b content). Type A harbors two hydrophobic subunits with two hemes b (Hägerhäll and Hederstedt 1996). Type A enzymes oxidize succinate, using methylmenaquinone (thermoplasmaquinone) as electron acceptor, and operate as succinate:quinone reductases (SQR) in vivo. SQRs belonging to type B harbor three subunits, containing two hemes b. This type of enzyme is known to operate in both directions with the MK/MKH2 pool as electron acceptor/donor (Hägerhäll 1997). SQRs belonging to type C, with two membranous subunits and one heme b (Yankovskaya et al. 2003), also operate in both directions, but exclusively with high potential quinones, such as ubiquinone (UQ) (Hägerhäll and Hederstedt 1996). Type D enzymes represent QFRs with two hydrophobic subunits, which lack heme cofactors (Hägerhäll and Hederstedt 1996; Iverson et al. 1999).
The four types of SQRs can also be categorized into three functional classes, based on their quinone specificity and their in vivo function (Lancaster 2004). Subclass 1 represents SQRs, oxidizing succinate and reducing high potential quinones, such as UQ. QFRs couple fumarate reduction to the oxidation of a low-potential quinol and belong to subclass 2. Enzymes belonging to subclass 3 represent SQRs, which oxidize succinate and reduce low-potential quinones (Lancaster 2004). Note that enzymes of subclass 1 and 2 catalyze exergonic reactions, while the reaction of subclass 3 enzymes is endergonic (under standard conditions at pH 7.0).
Members of different types and classes of the SQR family are broadly distributed in the bacterial kingdom, reflecting their high variability in terms of substrate specificity and modes of energy conversion (Unden and Dünnwald 2008). We searched for different SQR types in representatives from bacterial phyla (supplementary material table S1). QFRs oxidizing low-potential quinols, which are composed of three subunits and contain two hemes b (type B), are present in the phyla Chlorobiota, Bacteroidota, Actinomycetota, Thermodesulfobacteriota and Campylobacterota. Typical examples are QFR from Desulfovibrio gigas, a member of the Deltaproteobacteria (phylum Thermodesulfobacteriota), and Wolinella succinogenes, a member of the Epsilonproteobacteria (phylum Campylobacterota). SQRs oxidizing succinate and reducing high potential quinones are present in Alpha-, Beta- and Gammaproteobacteria. Some representatives from the Gammaproteobacteria comprise both UQ and MK, enabling the organism to utilize either succinate or fumarate prevalent under different environmental conditions with adequate catalytic pathways. Consequently, these organisms encode both SQR and QFR, which are expressed differentially. Typically, their QFRs are type D enzymes (harboring two hydrophobic subunits but no heme), oxidizing low-potential quinols and reducing fumarate (Hägerhäll 1997). Bacteria belonging to Bacillota operate SQRs, which oxidize succinate and reduce low-potential quinones in a reaction that is endergonic under standard conditions at pH 7.0.
Prevotella bryantii and Prevotella bivia belong to the family Prevotellaceae, which is assigned to the phylum Bacteroidota (Avgustin et al. 1997). These obligate anaerobes contain MK as the only reported quinone species (Schleicher et al. 2021a). They comprise a type B QFR with three subunits and two hemes b, which preferentially oxidizes menaquinol (MKH2) under reduction of fumarate in vivo.
Selected organisms of the phylum Bacteroidota (Bacteroides fragilis, Prevotella bryantii, Prevotella bivia) and members of the Gammaproteobacteria (Haemophilus influenzae) contain both NQR and QFR (supplementary table S1 and table S2). It seems likely that B. fragilis and H. influenzae may utilize both enzymes to re-oxidize NADH concomitant with the build-up of an electrochemical membrane potential as shown for P. bryantii (Schleicher et al. 2021a) and P. bivia (Schleicher et al. 2021b).
Conclusion
The NQR and QFR represent the key enzymes of energy conservation in P. bryantii. Here, the reduction of fumarate to succinate using NADH as electron donor is coupled to chemiosmotic energy conservation by SMF generation with menaquinone as redox carrier. From 3D structural models of Pb-NQR and Pb-QFR, routes for electron transfer and substrate binding sites are predicted. This mode of energy conservation is not only important for P. bryantii, but is likely to occur in other bacteria operating both NQR and QFR.
Abbreviations
- NQR:
-
Na+-translocating NADH:quinone oxidoreductase
- QFR:
-
Quinol:fumarate reductase
- SQR:
-
Succinate:quinone reductase
- FNO:
-
Ferredoxin:NAD+ oxidoreductase
- RNF:
-
Rhodobacter nitrogen fixation complex
- MK:
-
Menaquinone
- UQ:
-
Ubiquinone
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Funding by the Deutsche Forschungsgemeinschaft to Julia Steuber and Jana Seifert (Project No. 327953272) is gratefully acknowledged.
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Open Access funding enabled and organized by Projekt DEAL. Funding was provided by the Deutsche Forschungsgemeinschaft to Julia Steuber and Jana Seifert (Project No. 327953272).
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All authors contributed to the study conception and design. Analyses were performed by LS, J-LH and SH. The first draft of the manuscript was written by LS, J-LH and SH, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Hau, JL., Schleicher, L., Herdan, S. et al. Functionality of the Na+-translocating NADH:quinone oxidoreductase and quinol:fumarate reductase from Prevotella bryantii inferred from homology modeling. Arch Microbiol 206, 32 (2024). https://doi.org/10.1007/s00203-023-03769-5
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DOI: https://doi.org/10.1007/s00203-023-03769-5