Abstract
The processes that lead to a successful invasion are complex. Here, we investigated life history characteristics potentially explaining the invasion success of Potamopyrgus antipodarum, a small parthenogenetic and ovoviviparous freshwater snail that was recently added to the top “hundred worst” alien species in Europe. We monitored monthly, over the course of 1 year, shell size, number of brooded embryos, and the presence of castrating parasites at three Northeast German sites: a lake (the Kiessee), a stream (Hohen Sprenz), and a small spring brook (Rügen) (N = 1165). We found that (1) despite sharing the same clonal lineage, drastic differences in space and time for size and fecundity were observed, and these differences were linked to specific environmental variables (water temperature, salinity, and current); (2) P. antipodarum reproduces all year around, except at one of our sites, the spring Rügen, where the reproduction was seasonal; (3) none of our dissected specimens was infected by parasites. Together with ovoviviparity and the ability to reproduce parthenogenetically while being released from parasite pressure, the ability to adapt readily to a wide range of habitat conditions is likely paramount for the invasive success of P. antipodarum.
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Introduction
Biological invasions represent a major ecological and economic threat (Pimentel et al., 2006; Nentwig, 2007) and are even viewed as a component of global change (Vitousek et al., 1996). Invasive species have often been attributed with specific life history characteristics. For instance, once the transportation and establishment phase have been overcome (Sakai et al., 2001), high growth rate and reproduction often allow for rapid spread of invasive species by outcompeting native populations. Astounding examples comprise the Nile perch Lates niloticus (Linnaeus, 1758) in Lake Victoria (Goldschmidt, 1996) and the zebra mussel Dreissena polymorpha (Pallas, 1771) in the northern USA (Caraco et al., 1997) that caused the extinction of native species and profound ecological changes. The adaptability to novel environments has also been considered a key feature in the range expansion success of invasive species (e.g., Eriksen et al., 2012; Mccann et al., 2014; Verhaegen et al., 2018a), including the adaptation of their life history traits (e.g., Dlugosch & Parker, 2008; Jaspers et al., 2014; Li et al., 2015). Studying the adaptation of life history traits of invasive species to their new habitat is, therefore, crucial for two reasons. First of all, it is essential to reveal predispositions of species to become successful invaders or pioneers (Sakai et al., 2001) to prevent, manage, and mitigate biological invasions. Second, invasive species can be used as models providing insights on how organisms will cope with the consequences of current human-enhanced environmental change, as by definition the habitat they invade is new (Sakai et al., 2001).
In the present study, we investigated the spatial and temporal fluctuations in the life history traits size and fecundity of small invasive fresh and brackishwater snail Potamopyrgus antipodarum (Gray, 1843) in northern Germany. Commonly called the New Zealand mud snail, this gastropod is native to New Zealand, but has successfully invaded fresh and brackish waters in Australia, Europe, Japan, the USA, and Chile within the last 180 years (Smith, 1889; Alonso & Castro-Díez, 2012; Collado, 2014). Mixed populations of sexually reproducing diploid males and females coexist in the native range with parthenogenetically reproducing polyploids which are almost all females (Dybdahl & Lively, 1995; Neiman et al., 2011). Interestingly, in the entire invaded range, however, only these clonally reproducing females can be found (Hauser et al., 1992; Hughes, 1996; Jacobsen et al., 1996; Gangloff, 1998; Weetman et al., 2002; Städler et al., 2005; Verhaegen et al., 2018a). These invasive lineages were found only by few individuals: in Europe for instance, only two mitochondrial lineages (Städler et al., 2005; Verhaegen et al., 2018a) have been identified. On the nuclear level, the diversity was somewhat higher, but the divergence estimated based on microsatellites (Weetman et al., 2002) or SNPs was fairly low suggesting post-invasion diversification (Verhaegen et al., 2018a).
The impact of this snail on its invaded habitat varies from place to place, which is possibly explained by different densities this snail can reach (Alonso & Castro-Díez, 2012). In Australia, for instance, a positive relationship has been found between densities of P. antipodarum and native benthic fauna (Schreiber et al., 2002), whereas in the USA and Chile a negative effect was shown on endemic macroinvertebrate communities (Kerans et al., 2005), including snails (Richards, 2004; Riley et al., 2008; Collado et al., 2019a, b). In Europe, P. antipodarum has been recently added to the top “hundred worst” alien species (rank 42), among which it is the third “worst alien” mollusc, after D. polymorpha and the golden apple snail Pomacea canaliculata (Lamarck, 1819) (Nentwig et al., 2018). The high rank of P. antipodarum was mostly attributed to its major environmental impact on ecosystems (e.g., Hall et al., 2003) and other species through competition, a medium impact on vegetation through herbivory, and a major socio-economic impact on animal production [e.g., on weight and health of rainbow trout (Vinson & Baker, 2008)], although the literature investigated for the scoring was obviously not restricted to European populations (Laverty et al., 2015). Although P. antipodarum can dominate gastropod and mollusc communities in Europe (e.g., Gérard et al., 2003; Lewin & Smoliński, 2006), only a weak effect on macroinvertebrate communities has been reported so far (Múrria et al., 2008; Schmidlin et al., 2012).
The invasive success of P. antipodarum has been attributed to various factors. Successful transportation and establishment of P. antipodarum has been associated with a wide tolerance to physical and chemical parameters including high salinities (e.g., Gérard et al., 2003; Leclair & Cheng, 2011), a wide range of temperatures (e.g., Winterbourn, 1969; Hylleberg & Siegismund, 1987), and desiccation (e.g., Richards et al., 2004; Lysne & Koetsier, 2006). Another cause of its success is the so-called “enemy release” hypothesis (Darwin, 1859). In its native range, P. antipodarum is subjected to strong selective pressure by castrating parasitic trematodes (e.g., Winterbourn, 1974; Hechinger, 2012) locally favoring sexual over asexual reproduction (Bell, 1982; Jokela et al., 2009; Neiman & Koskella, 2009; Neiman et al., 2017). In the invaded range, however, infections are extremely rare (e.g., Zbikowski & Zbikowska, 2009; Gérard et al., 2017; Verhaegen et al., 2018a), this “release” allowing P. antipodarum to fully exploit the advantages of parthenogenetic reproduction (Lively, 1992): reproductive assurance (i.e., no need for finding mating partners) (Jain, 1976; Lloyd, 1979; Gerritsen, 1980) and no “two-fold” cost related to production of males (Maynard Smith, 1971; Gibson et al., 2017). In addition, rapid population growth, fast spread, and high competition for primary resources have been attributed to its high fecundity (Real, 1971; Lassen, 1979; Alonso & Castro-Díez, 2008). Ovoviviparity is certainly another factor facilitating successful invasion (Collado, 2014). At last, the low genetic diversity and lack of recombination related to asexual reproduction are further compensated by this invasive snail through its ability to adapt its shell morphology, which is linked to fecundity, to the environment through phenotypic plasticity (Kistner & Dybdahl, 2013; Verhaegen et al., 2018a).
Here, we monitored shell size and fecundity of three populations of P. antipodarum in Northeast Germany in monthly intervals over the course of 1 year. We tested the following three expectations that would explain the invasion success of this clonal invader: (1) despite being genetically practically identical and being geographically closely located, we expected to observe differences in the monitored life history traits among populations, and these differences to reflect temporal and spatial environmental changes encountered in their markedly different habitats—a lake, a stream, and a shallow spring brook. If differences among these populations were observed, these would most likely be due to phenotypic plasticity (Dybdahl & Kane, 2005; Gust et al., 2011; Kistner & Dybdahl, 2013; McKenzie et al., 2013; Verhaegen et al., 2018a, b) that shows the ability of this species to adapt its life history traits to the environment; (2) we expected P. antipodarum to reproduce all year around (e.g., Schreiber et al., 1998, McKenzie et al., 2013) and therefore to find both brooding females and the presence of juveniles every month; 3) according to the “enemy release” hypothesis and previous studies from the invaded range (e.g., Zbikowski & Zbikowska, 2009, Verhaegen et al., 2018a), the absence of castrated parasites was expected. Our findings were then compared to those in the species’ native range (Winterbourn, 1970a), and in other invaded territories, namely in Australia (Schreiber et al., 1998) and in the USA (McKenzie et al., 2013). Despite P. antipodarum being part of the top “hundred worst” alien species present in Europe, this is the first study that monitored in situ fluctuations in life history traits for European populations over a year. It is also the first in situ study designed with prior genetic knowledge in order to assure comparable genetic background (Verhaegen et al., 2018a).
Materials and methods
Collection
Potamopyrgus antipodarum was collected monthly between February 2017 and January 2018 (excluding October 2017) from three Northeast German sites: a lake, the Kiessee in Jarmen (referred to hereafter as Jarmen, N 53° 55′ 44.5, E 13° 18′ 60.0), a stream, the Mühlbach in Hohen Sprenz (Hohen Sprenz, N 53° 55′ 24.2, E 12° 11′ 57.7), and a small, shallow spring brook, located in Quellsumpf Ziegensteine, Klein Stresow, on the island of Rügen (Rügen, N 54°21′ 23.7, E 13° 36′ 27.0) (Fig. 1). These locations were identical to the sites with acronyms DEJAR, DEHOB/T, and DERUG, respectively, from Verhaegen et al. (2018a), who showed that snails collected at these locations during summer 2015 and/or 2016 shared the same mitochondrial haplotype. The populations from Hohen Sprenz and Jarmen also shared the same nuclear SNP genotypes (62 neutral loci); however, the one from Rügen differed slightly by seven substitutions (Verhaegen et al., 2018a). Snails were collected with a small dip net from the bottom or hard substrates at a maximum depth of 50 cm and fixed immediately in 80% ethanol. On each sampling occasion, the following environmental parameters were recorded: water temperature (°C) and salinity (ppm) using a water tester (Milato®, Germany), water current [lentic (i.e., without flow) vs. lotic (i.e., with flow)], and shade coverage (%).
Measurement of life history traits
The shells of the snails were individually photographed under a Carl Zeiss Discovery V20 microscope with a Plan Apo S × 0.63 objective and an AxioCam MRc camera, by identically positioning their aperture facing up and the coiling axis oriented horizontally on a silicone support. Shell height (Fig. 2) was then measured with the Axio Vision microscope software (Zeiss). To ensure that the variation in size was unrelated to manipulative error, this part was only conducted by one of us, KvJ, and a repeatability test was performed by photographing and measuring the same 29 snails twice 2 weeks apart and testing the difference in shell height with a two-sample paired t test (t = − 1.427, P = 0.165). Individuals were assigned to one of the following age classes: adult, subadult, and juvenile. An adult snail was defined as a fully grown individual displaying a continuous apertural lip (Verhaegen et al., 2018a). Snails displaying a discontinuous apertural lip and a shell height of 70% or above than the average shell height of the collected adults were classified as subadult, whereas smaller snails were classified as juvenile.
After being photographed and measured, the shells were dissolved in EDTA (0.5 M, pH 7.5) for 3 days to expose the soft bodies. The snails were then dissected under a microscope to determine their sex (absence of a penis for females, Winterbourn, 1970b) and the presence of parasites. The number of embryos as proxy for fecundity was counted for each female by opening their brooding pouches (see Fig. 24A in Haase, 2008).
Statistical analyses
Univariate statistics were conducted to compare differences in shell height and environmental parameters among localities and differences in the number of brooded embryos among localities and age classes. Generalized linear models (GLMs), that allow for both continuous and categorical explanatory variables and do not require normally distributed errors of the response variables, were used to examine the influence of environmental parameters and the sampling month on the life history traits. The influence on shell height of adult snails (N = 614) was assessed with a linear model (LM). Collinearity between the explanatory variables was detected by calculating pairwise Goodman and Kruskal’s tau (GK tau) measures, a method suited for both categorical and numerical variables, with the GKtauDataframe function implemented in the R package GoodmanKruskal v. 0.0.2 (Pearson, 2016). For pairs showing a strong association (GK tau values ~ 1), only one variable was maintained. The remaining explanatory variables tested in the model were as follows: month, temperature, salinity, and current. The influence on fecundity was tested with the same explanatory variables plus shell height with a GLM for Poisson-distributed errors. For this GLM, we only kept female adults brooding at least one embryo (N = 423) to meet the assumptions of the Poisson distribution.
The LM and the GLM were built with the lme4 v. 1. 1-19 package in R (Bates et al., 2015) by stepwise dropping explanatory variables based on type-II Wald Chi-square tests of the Anova function implemented in the car package. The positive or negative effects of the final remaining significant explanatory variables were visualized by means of plots using the predictorEffects function (Fox & Weisberg, 2018) of the effects v. 4.0-3 package (Fox, 2003). All statistical tests were executed in PAST v. 3.20 (Hammer et al., 2001) or in R v. 3.5.1 (R Core Team, 2013). Non-parametric tests were used if normal distributions were rejected by a Shapiro–Wilk test and P values were Bonferroni corrected in case of multiple testing (e.g., pairwise tests).
Results
Variation in life history traits
A total of 1165 snails were collected between February 2017 and January 2018. Of these, 614 were adults, 403 were subadults, and 148 were juveniles. These three age classes were found all year around at least at one sampling location (Table 1). All dissected snails were females uninfected by parasites.
The shell height of adult snails varied between 2.2 mm (sampled in Rügen, February 2017) and 5.6 mm (Hohen Sprenz, December 2017), with an annual median size of 3.9 mm. Significant differences in adults’ shell height were found among three locations (Kruskal–Wallis Chi2 = 356.3, P < 0.0001; pairwise Mann–Whitney U tests summarized in Table 2), with the largest snails observed in Hohen Sprenz (median = 4.3 mm), followed by Jarmen (4.0 mm) and Rügen (3.5 mm) (Fig. 3a). In a similar fashion, the shell height of the subadult snails varied significantly among locations (Chi2 = 189.9, P < 0.0001; Table 2), with the largest snails found in Hohen Sprenz (median = 3.9 mm), followed by Jarmen (3.6 mm) and Rügen (3.1 mm). Brooding subadult snails (“with” embryos) were significantly larger (median = 3.8 mm) compared to non-reproducing subadults (“without” embryos; 3.2 mm; U = 4590.5, P < 0.0001). The size of brooding subadult snails varied again significantly among localities (Hohen Sprenz = 4.1 mm, Jarmen = 3.8 mm, Rügen = 3.4 mm; Chi2 = 56.5, P < 0.0001; Table 2; Fig. 4).
The number of embryos brooded by adult snails varied between zero and 41 (Hohen Sprenz, September 2017) and between zero and 28 (Hohen Sprenz, December 2017) in subadults. Adult snails brooded significantly more embryos (median = 7) compared to subadults (median = 0) (U = 68862, P < 0.0001). The annual total number of brooded embryos varied significantly among localities, both for adult (Chi2 = 204.7, P < 0.0001) and subadult (Chi2 = 54.8, P < 0.0001) snails. The highest number of embryos was found in Hohen Sprenz (median adults = 14, subadults = 6), followed by Jarmen (adults = 9, subadults = 0) and Rügen (adults = 0, subadults = 0) (Table 3; Fig. 3b).
Environmental and seasonal effects on life history traits
Water temperatures varied between 2.2 (Jarmen, February 2017) and 21.6°C (Hohen Sprenz, August 2017), with the coldest temperatures recorded between December and March (6.1 ± 2.1°C—mean ± SD) and the warmest between June and September (18.8 ± 2.9°C) (Fig. 5a). Potamopyrgus antipodarum were observed crawling at the bottom of the sampling locations throughout the year, including during the coldest months when the water surface was frozen. The annual mean water temperature was highest in Hohen Sprenz (13.1 ± 6.9°C), followed by Jarmen (12.2 ± 6.8°C), and the lowest in Rügen (11.7 ± 4.0°C). Salinity varied between 130 (Hohen Sprenz, February 2017) and 379 ppm (Jarmen, September 2017), but showed no clear seasonal pattern (Fig. 5b). The annual mean salinity was the highest in Rügen (287.3 ± 36.1 ppm), followed by Jarmen (265 ± 65.5), and the lowest in Hohen Sprenz (180.3 ± 29.76).
Sampling month (Fig. 6), current, temperature, and salinity had significant effects both on shell height and the number of brooded embryos of adult snails (Fig. 7, Supplementary Fig. S2, Table 4). Temperature had a positive and salinity a negative effect on the snails’ size and fecundity. Larger and more fecund snails were found in lentic sites compared to lotic ones. Larger snails also brooded significantly more embryos. Overall, the shell size stayed relatively constant throughout the year, with a peak observed in winter during the months December through January, whereas fecundity was low from February to May, and high throughout the rest of the year, with a peak observed in June (and a second one in Hohen Sprenz in January; Figs. 6, 7). Monthly patterns in fecundity varied, however, among localities (Fig. 6). Adult snails were observed to brood embryos year-round in Hohen Sprenz and Jarmen, with a more pronounced increase toward summer in Jarmen, whereas brooding adults in Rügen were predominately found in summer between June and September, but were nearly absent the rest of the year. In Hohen Sprenz, subadult snails showed a similar annual pattern compared to the adults, whereas in Jarmen and Rügen, the seasonal fecundity pattern was even more pronounced, with brooding occurring only from April to August in Jarmen, and half as long, from June to August, in Rügen.
Discussion
In order to explain the invasion success of P. antipodarum, we monitored shell size and fecundity of three of its Northeast German populations in monthly intervals over the course of one year. We found the following as expected: 1) drastic differences in space and time for these life history traits, and these differences were linked to specific environmental variables and 2) none of our dissected specimens to be infected by castrating parasites. Our expectation that invasive populations of P. antipodarum would be reproducing all year around was met at all but one sampling site, site Rügen, where it was seasonal.
Environmental effects on life history traits
Shell size and fecundity of adults varied among locations, despite the close relationship of the snails and their clonal nature (Verhaegen et al., 2018a). The observed variation was explained as response to specific environmental factors. The observed positive effect of temperature was in accordance with previous studies on size (Verhaegen et al., 2018b), growth rate (Dybdahl & Kane, 2005), and fecundity (Dybdahl & Kane, 2005; Gust et al., 2011; McKenzie et al., 2013; Verhaegen et al., 2018b). The positive effect of temperature on fecundity and size has, however, an upper limit following an optimum curve, as was experimentally shown (Macken et al., 2012; Bennett et al., 2015). The temperature range we measured (2.2–21.6°C) was within the experimentally tested tolerance range of 0–28°C (Winterbourn, 1969; Hylleberg & Siegismund, 1987; Bennett et al., 2015) and below the upper limit for fecundity (Macken et al., 2012) and growth (Bennett et al., 2015). Other annual temperatures measured in situ where living snails were found year-round ranged from 0 to 20°C in Denmark (Lumbye & Lumbye, 1965), 2–19°C in a stream in Colorado (McKenzie et al., 2013), and from 11 to 23°C in an Australian lake (Schreiber et al., 1998). We collected living snails from the ice-covered lake in Jarmen in February 2017 at a water temperature of 2.2°C. The collection of living snails from an ice-covered lake at 1.5°C was so far only reported from the Netherlands (Dorgelo, 1987). However, survival rates apparently drop dramatically once temperatures drop below 0°C (Hylleberg & Siegismund, 1987).
Salinity had a negative effect on the measured traits, which was in contrast to some of the previous studies. A negative effect on fecundity, but a positive effect on size were reported for native freshwater populations (Verhaegen et al., 2018b), whereas no effect was found in European brackish and freshwater populations (Verhaegen et al., 2018a). Herbst et al. (2008) experimentally showed a positive effect of conductivity (ranging from 25 to 200 μS/cm) on growth, suggesting the need of calcium ions for shell formation. The discrepancies to previous findings is perhaps best explained by a study of Levri et al. (2014), who showed that the effect of conductivity on growth rate was lineage dependent among American populations.
Current was the third factor influencing the measured life history traits, with snails in lentic habitats being larger, but breeding slightly less embryos compared to those from lotic habitats. Usually, the opposite, i.e., larger snails with increasing flow, has been reported both in the native (Haase, 2003) and invaded (Verhaegen et al., 2018a) ranges. This difference is probably due to the lotic habitats, Hohen Sprenz (a stream) and Rügen (a spring), being much smaller compared to the lentic site Jarmen (a lake). Indeed, Verhaegen et al. (2018b) showed the largest P. antipodarum to be found in lakes and large streams, the size to decrease with stream size, and the smallest snails to be found in springs. The effects of temperature, salinity, and water body type we observed explain why the smallest and less fecund individuals were found in Rügen, a small spring where the coldest annual temperatures and highest annual salinities were recorded. The lower fecundity was shown for lotic habitats before (Verhaegen et al., 2018a) and likely represents a trade-off with living in habitats where more resources have to be allocated to fight dislodgment risk due to increased drag and lift forces (Verhaegen et al., 2019).
The shell size of adult snails had a positive effect on the number of embryos they brooded, as demonstrated previously (McKenzie et al., 2013; Verhaegen et al., 2018a, b). As expected, the size of brooding subadults varied also significantly among locations. Female size at maturity was already known to vary among native populations (Jokela et al., 1997), early reproduction being hypothesized to be a response against high parasitic pressures (Jokela & Lively, 1995). Here, in the invaded range, size of both adult and brooding subadult snails was the smallest in Rügen. This sampling location obviously provided the least favorable environmental conditions for both size and fecundity (see above). By modifying the size at sexual maturity, P. antipodarum appears to ensure reproduction also in less suitable habitats not supporting large body size, however, at the cost of smaller brood size. Early sexual maturity is also known to be another life history trait favoring the range expansion success of invasive species [e.g., in invasive pines (Richardson et al., 1990)]. We, therefore, suggest early sexual maturity and the adaptation of size at sexual maturity to be added to the list of functional traits explaining the invasive success of P. antipodarum (Alonso & Castro-Díez, 2008).
The adaptation of life history traits to changing environments has been a key feature in the range expansion success of invasive species (e.g., Dlugosch & Parker, 2008; Jaspers et al., 2014; Li et al., 2015). Any forms of adaptation can be mediated through natural selection of beneficial alleles or genotypes introduced into a population through mutation, migration, or recombination (Carja et al., 2014), and/or by phenotypic plasticity (Sultan, 1995). In the case of asexual species or lineages reproducing without recombination, which is the case for the ameiotic parthenogenetic invasive lineages of P. antipodarum, the adaptation of life history traits as observed here, should mostly be the result of phenotypic plasticity (e.g., Verhaegen et al., 2018a, b). Besides the measured environmental parameters, other biotic factors and pollutants are also known to influence life history traits in P. antipodarum. For instance, fecundity can be affected by population density (e.g., Neiman et al., 2013; Zachar & Neiman, 2013) or anthropogenic chemicals interfering with reproductive endocrinology (e.g., Duft et al., 2003; Jobling et al., 2003; Geiß et al., 2017). Therefore, it would be interesting to extend this study and test the effect of additional factors on the variability of plasticity in life history traits.
Temporal fluctuations
Size, represented by shell height, and fecundity were also significantly affected by the sampling month. The size of adult snails slightly increased throughout the year to reach a peak in January (Fig. 7). In Denmark, however, a seasonal increase in average weight of unspecified age class was reported toward the summer months (Lumbye & Lumbye, 1965), but the annual variation in shell size was not investigated before. Annual fecundity patterns of adults and subadults varied among locations. At two of our three locations, adult snails brooded embryos year-round. In Rügen, however, brooding adults were almost only found during the warmest months, from June to September. In the literature, adult snails are usually reported to brood throughout the year [e.g., in New Zealand (Winterbourn, 1970a) and in the USA (McKenzie et al., 2013)], with the exception of an Australian site where brooding adults were absent in one month (Schreiber et al., 1998). This is, thus, the first report of a population that does not reproduce throughout most of the year. The number of brooded embryos by adults and subadults varied seasonally, in concordance with what was found on other continents (Winterbourn, 1970a; Schreiber et al., 1998; McKenzie et al., 2013). The duration of the period of maximum fecundity varied, however, among locations and age classes. Adults showed a longer high-reproductive season than subadults, and this high-reproductive season of adult snails from Hohen Sprenz was twice as long compared to that on Rügen lasting from June to January. A less pronounced seasonality in the life history traits such as growth rate and reproduction is obvious at sites with a more benign climate year-round (Bennett et al., 2015). We observed juveniles throughout the year at least at one of the three sites. The occasional absence in our collections was probably due to our sampling method. Year-round recruitment in this species has been shown both in the native (Winterbourn, 1970a) and invaded ranges (Schreiber et al., 1998; Bennett et al., 2015). Although not investigated in this study, it is important to note that densities of P. antipodarum have also been reported to fluctuate through time and among localities (e.g., Lumbye & Lumbye, 1965; Dorgelo, 1987; Schreiber et al., 1998; Bennett et al., 2015) and can influence the growth rate and fecundity in P. antipodarum as well (e.g., Neiman, 2006; Neiman et al., 2013; Sieratowicz et al., 2013; Zachar & Neiman, 2013).
Sex and parasitism in the invaded range
All dissected snails were females and none were infected by parasites. This is what is typically found in the invaded range of P. antipodarum. Previous studies found no infected snails (N = 425) in Belgium, Germany, or the Netherlands (Verhaegen et al., 2018a), an 0.01–0.02% infection rate in Poland (Zbikowski & Zbikowska, 2009; Cichy et al., 2017), 0–0.10% in the USA (Adema et al., 2009; McKenzie et al., 2013), 0.22–0.48% in France (Gérard & Dussart, 2003; Gérard & Le Lannic, 2003; Gérard et al., 2003), 1.33% in the United Kingdom (UK) (Evans et al., 1981), and 3.6% in an Australian lake (Schreiber et al., 1998). The presence of males in the invaded range is even rarer: although the percentage of males in Europe ranged from zero to 5% in earlier studies (Wallace, 1985), no males were recently reported in Belgium, Germany, the Netherlands, Poland, and the USA (Cichy et al., 2017; McKenzie et al., 2013; Verhaegen et al., 2018a), only one male individual out of 5,774 was found in France (Gérard et al., 2003), and 1.1% of males were found in Australia (Schreiber et al., 1998). Our observations support yet another factor explaining the invasive success of P. antipodarum: the combination of the “Enemy release” and the ability to reproduce parthenogenetically (Lively, 1992).
Conclusion
The processes that lead to a successful invasion are complex. In situations where natural predators are lacking, a competitive invader is determined by specific r-strategy life history traits (e.g., fast growth, high reproduction) and phenotypic plasticity (Sax & Brown, 2000; Facon et al., 2006; Sorte et al., 2010). An additional advantage is given to invaders that reproduce asexually or self-fertilize and are, therefore, not limited by the difficulties of finding a mate at the early stages of colonization (Tobin et al., 2011). Here, we characterized the growth and reproduction of such an asexually reproducing invader, P. antipodarum, by monitoring monthly, over the course of a year, shell height and number of brooded embryos in three Northeast German populations. Castrating parasites are typically lacking in its invaded range and were absent as well from all our dissected specimens. Our expectation that P. antipodarum would reproduce all year around, which was observed for other invaded continents (Schreiber et al., 1998; McKenzie et al., 2013) and the native range (Winterbourn, 1970a), was only met at two of our three monitored sampling sites. Brooding females at the small spring brook site Rügen were only found predominantly during the summer months. As expected, however, we found drastic differences in adult size, size at sexual maturity, and fecundity among sampling locations and over time, despite the high genetic similarity of these clonal lineages (Verhaegen et al., 2018a) and the small geographical scale. The observed spatial and temporal variation of these life history traits was obviously due to adaptation to the differing local habitat conditions and most likely an environment-induced plastic response. Together with ovoviviparity and the ability to reproduce parthenogenetically while being released from parasite pressure, the ability to adapt readily to a wide range of habitat conditions is likely paramount for the invasive success of P. antipodarum.
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Acknowledgements
We thank D. Treschnak, S. Fregin, and N. Lieker for their assistance with fieldwork. G. Uhl is thanked for providing access to the Zeiss microscope. We are grateful to W. Matzke of the Biosphärenreservatsamt Südost-Rügen for providing a collecting permit. We also thank G. Collado and two anonymous reviewers for their constructive comments on previous versions of the manuscript. This study was conducted in the frame of the Research Training Group 2010 RESPONSE funded by the Deutsche Forschungsgemeinschaft (DFG). Open Access funding provided by Projekt DEAL.
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GV and MH—Fieldwork. KvJ—Dissections and measurements. GV, MH, and KvJ—Statistical analyses. GV, MH, and KvJ—Manuscript; GV and MH—Study concept and experimental design
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Supplementary Fig. S1 Effects of the significant explanatory variables on shell height and number of brooded embryos of adult females according to our (generalized) linear models. Supplementary material 1 (PDF 51 kb)
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Verhaegen, G., von Jungmeister, K. & Haase, M. Life history variation in space and time: environmental and seasonal responses of a parthenogenetic invasive freshwater snail in northern Germany. Hydrobiologia 848, 2153–2168 (2021). https://doi.org/10.1007/s10750-020-04333-8
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DOI: https://doi.org/10.1007/s10750-020-04333-8