Abstract
Modulation of the plant defense response by bioactive molecules is of increasing interest. However, despite plant cell lipids being one of the major cellular components, their role in plant immunity remains elusive. We found that the exogenous application of the cell-membrane localized phospholipid lyso-phosphatidylethanolamine (LPE) reprograms the plant transcript profile in favor of defense-associated genes thereby priming the plant immune system. Exogenous LPE application to different Arabidopsis accessions increases resistance against the necrotrophic pathogens, Botrytis cinerea and Cochliobolus heterostrophus. We found that the immunity-promoting effect of LPE is repealed in the jasmonic acid (JA) receptor mutant coi1, but multiplied in the JA-hypersensitive mutant feronia (fer-4). The JA-signaling repressor JAZ1 is degraded following LPE administration, suggesting that JA-signaling is promoted by LPE. Following LPE-treatment, reactive oxygen species (ROS) accumulation is affected in coi1 and fer-4. Moreover, FER signaling inhibitors of the RALF family are strongly expressed after LPE application, and RALF23 is internalized in stress granules, suggesting the LPE-mediated repression of FER-signaling by promoting RALF function. The in-situ increase of LPE-abundance in the LPE-catabolic mutants lpeat1 and lpeat2 elevates plant resistance to B. cinerea, in contrast to the endogenous LPE-deficient mutant pla2-alpha. We show that LPE increases plant resistance against necrotrophs by promoting JA-signaling and ROS-homeostasis, thereby paving the way for the LPE-targeted genomic engineering of crops to raise their ability to resist biotic threats.
Key message
Modulation of the plant defense response by bioactive molecules is of increasing interest. However, despite plant cell lipids being one of the major cellular components, their role in plant immunity remains elusive. We show that LPE increases plant resistance against necrotrophs by promoting JA-signaling and ROS-homeostasis, thereby paving the way for the LPE-targeted genomic engineering of crops to raise their ability to resist biotic threats.
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Introduction
Plant development is highly plastic in its response to a changing environment. Plants can trigger specific differentiation programs, promote growth over differentiation, or favor defense strategies in response to biotic stresses. Although the molecular mechanisms by which plants integrate environmental and endogenous signals are not completely understood, there is a high degree of conservation between the various elements of plant signaling pathways (Marin-de la Rosa et al. 2015).
Plant-signaling lipids comprise a vast array of lipid classes including, fatty acid, phosphatidic acid, phospholipids, diacylglycerol, oxylipin, inositol phosphate, sphingolipid and N–acylethanolamine. Phospholipids participate in a wide spectrum of biological functions, including signal transduction, the storage of energy, and enabling the structural dynamics and integrity of cell membranes.
Lyso-phosphatidylethanolamine (LPE) is a minor component of the cell-membrane localized lipids and originates from the precursor phosphatidylethanolamine (PE). Phospholipase A2 hydrolyzes, the structural phospholipid PE, thereby generating LPE. Phosphoethanolamine is the precursor of PE and phosphatidylcholine (PC) and the rate-limiting enzyme PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE 1 (PECT1) modulates the PC: PE ratio in Arabidopsis (Canonne et al. 2011; Lee et al. 2005). The knockout of PECT1 impacts the PC: PE ratio to the disadvantage of PE (Mizoi et al. 2006). The two enzymes LYSOPHOSPHATIDYLETHANOLAMINE ACYLTRANSFERASE1 (LPEAT1) and LPEAT2 acetylate LPE with acyl-coenzyme A (Jasieniecka-Gazarkiewicz et al. 2017). The disruption of LPEAT1 and LPEAT2 increases the LPE content in leaves.
Lipid profiling of Arabidopsis plants revealed that PE contributes to around 6.45% and LPE approximately 0.026% of the total quantity of leaf lipids. LPE is a glycerolipid that is exogenously applied on a wide range of crops, e.g. green pepper, sweet cherry, strawberries, and tomatoes (Farag & Palta 1993; Amaro & Almeida 2013; Ozgen et al. 2015) to delay early senescence while simultaneously accelerating fruit ripening (Farag & Palta 1993; Ryu et al. 1997; Hong et al. 2009). Recently, we found that the application of exogenous LPE increases plant resistance against the hemibiotrophic pathogen Pseudomonas syringae (Volz et al. 2021).
Here, we show that the transcriptional profile of LPE-treated plants is enriched in immunity-associated transcripts. LPE modulates the ROS-homeostasis and JA-signaling thereby priming the plant for infection by necrotrophic invaders.
Results
LPE application reprograms defense-associated gene expression
While on the hunt for factors that activate the plant immune system, we identified a cell-membrane localized phospholipid LPE as a putative enhancer of defense-associated processes. We spray-applied LPE at 24 h before Botrytis cinerea inoculation and determined the lesion formation in two Arabidopsis thaliana accessions Columbia (Col) and Wasselevskaja (Ws). We found that the lesion formation at 72 h post inoculation (hpi) was significantly reduced in Col and Ws following LPE pretreatment (Fig. 1A). This result suggests that LPE pretreatment interferes with B. cinerea-triggered plant cell death and host-colonization. Furthermore, we analyzed whether LPE pretreatment also promotes resistance against the necrotrophic pathogen Cochliobolus heterostrophus C4 (Volz et al. 2020). We found that both Arabidopsis accessions exhibited diminished lesion formation at 72 hpi compared to the mock-treated control (Fig. 1B). These outcomes indicate a plant immunity-promoting effect of LPE pretreatment against necrotrophic pathogens.
To elucidate host-molecular processes that might be modulated by LPE treatment, we analyzed the transcript profile at 6 h, and 24 h after LPE application compared to the mock-treated control. When taking into account the expression profiles for all the detected transcripts a close to linear correlation coefficient (Pearson-correlation) was obtained with mock vs. 6 h (0.932), mock vs. 24 h (0.929), and 6 h vs 24 h (0.859) (Fig. 1C, D). The strict correlation suggests that LPE does not affect general gene expression in an unbiased way, but rather influences subsets of genes in particular biological processes. Hierarchical clustering of differentially expressed genes (DEGs) (p < 0.01), by the use of normalized FPKM values, revealed distinct changes in gene expression patterns in the course of LPE perception. We identified 1914 genes that were upregulated and 1,111 that were downregulated 6 h after LPE administration, while after 24 h, 2938 genes showed elevated transcript levels and 1659 genes exhibited reduced expression levels (Fig. S1A, Table S1). Upregulated genes at 6 h after LPE application were grouped into gene-ontology (GO) terms showing the upregulation of response to biotic stimulus, bacterium, fungus wounding, chitin, and jasmonic acid (Fig. 1E, S1D). Notably, marker genes for jasmonic acid signaling showed highly elevated transcript levels, e.g. PDF1.2a/b/c, PDF1.3, ORA59, but also genes involved in PTI response, salicylic acid and senescence were upregulated, such as PR1, FRK1, WRKY53, SAG13, and SAG21. By contrast, GO term analysis for downregulated genes at 6 h after LPE application highlighted gene functions in processes that contribute to growth, development, and metabolic processes (Fig. 1F).
This analysis suggests that LPE perception triggers regulatory pathways that favor defense-associated processes over plant growth. Furthermore, gene-regulatory networks are stimulated by LPE that contribute to defense against necrotrophic pathogens.
Furthermore, we identified GO terms for genes that were significantly upregulated, these described gene functions in peptide metabolism, response to salt stress, oxidative stress aging, and bacterium at 24 h after LPE application (Fig. 2A, Table S2). Whereas, downregulated genes are classified into GO terms for developmental and metabolic process, gene expression and auxin response (Fig. 2B). Interestingly, cluster analysis revealed a major upregulation of defense-associated pathways at 6 h followed by a predominate downregulation at 24 h (Fig. 2C). We identified genes with a strong peak of upregulation at 6 h, whose encoded proteins catalyze rate-limiting steps in the JA-biosynthesis (AOC1, AOC2, AOS, JOX4) and JA-signaling pathway (ORA59, PDF1.2, JAZ6, PR4, and WAX1) (Fig. 2C, D, Fig. S1B). Genes that are predominately downregulated at 6 h and upregulated after 24 h following LPE administration contribute to small molecular metabolic and nucleotide processes, and response to light intensity and photosynthesis (Fig. 2F, G, Fig. S1C). Thus, it is conclusive that LPE amends the transcript profile predominately towards JA and ethylene coordinated immunity, while gene expression profiles that promote metabolic processes are neglected.
To analyze whether LPE perception triggers MAP kinase signaling pathways, we analyzed the phosphorylation of MPK3 and MPK6 which coordinate PTI and ETI in plant immunity (Meng & Zhang 2013). Previously, it was shown that the phospholipid, phosphatidic acid, binds to and triggers MPK3 and MPK6 phosphorylation in response to submergence-induced hypoxia (Zhou et al. 2022). This prompted us to analyze the phosphorylation of these immune MAP kinases at 0 h, 0.5 h, 6 h, and 24 h after LPE-treatment of 14-days-old seedlings (Fig. 2H). We could not detect changes in the phosphorylation status of MPK3 and MPK6 at the indicated time-points compared to mock-treated control which suggests that these immune-associated MAP kinase-signaling pathways play a minor role in the LPE-induced immunity.
Altered in-situ LPE-homeostasis impacts plant resistance
We questioned whether changed in-situ LPE levels increase plant resistance according to exogenous LPE application. To address this question, we analyzed the mutants of LYSOPHOSPHATIDYLETHANOLAMINE ACYLTRANSFERASE1 (LPEAT1) and LPEAT2. The lpeat1 and lpeat2 mutants were shown to have an increased in-situ homeostasis of LPE (Jasieniecka-Gazarkiewicz et al. 2017). We found that disease resistance of lpeat1 and lpeat2-defective plants infected with B. cinerea was increased compared to the control group (Fig. 2E). Conversely, we analyzed the mutant of the PHOSPHOLIPASE 2-ALPHA (PLA2-ALPHA) compromised in LPE biosynthesis and quantity deficiency (Lee et al. 2010; Wang 2005). The pla2-alpha mutant exhibited an increased susceptibility to B. cinerea infection compared to WT and lpeat1 and lpeat2 (Fig. 2E). Thus, we found that increased in-situ LPE levels reduce the susceptibility of the B. cinerea-infected plants according to the exogenous application. However, the diminished in-situ LPE levels increase the susceptibility and vulnerability of the host. Taken together, the above results indicate that the modification of in-situ LPE abundance directly impacts plant resistance reminiscent of the exogenous administration.
Hormonal signaling mutants show distinct susceptibility after LPE pretreatment
The transcript profile after LPE treatment revealed the enrichment of JA signaling genes which were previously shown to promote defense-response. Thus, we analyzed JA signaling mutants for changes in the immune-promoting effect given by LPE pretreatment and followed by B. cinerea infection. We characterized the two allelic mutants of the JA-receptor COI1 (coi1-21, coi-22 (He et al. 2012)). Both allelic coi1 mutants showed a higher susceptibility to B. cinerea after mock pretreatment compared to WT. After LPE pretreatment, a strong decrease in the B. cinerea infection could be observed in WT (Fig. 3A). However, both allelic coi1 mutants showed a susceptibility to B. cinerea after LPE-pretreatment that corresponds to the coi1 mock-treated control, indicating that JA-signaling is vital for the innate immunity-priming effect of LPE (Fig. 3A, B). To further analyze whether increased JA-signaling might promote the immunity-boosting effect of LPE, we included the fer-4 mutant (Guo et al. 2018; Escobar-Restrepo et al. 2007), which exhibited an elevated JA-response in an MYC2-dependent manner. Furthermore, we analyzed the COI1-overexpressor line (COI1ox) driven by the COI1 promoter in the WT plant. The mock-treated fer-4 and COI1ox lines exhibited reduced susceptibility to B. cinerea. After LPE pretreatment, we intriguingly found that COI1ox and fer-4 showed a further increased resistance to B. cinerea infection, when compared to LPE-pretreated WT plants (Fig. 3B). Together, these results indicate that the immune-promoting effect given by LPE pretreatment relies on JA-signaling and response. To analyze the impact of LPE on JA- and ET-signaling, we examined pivotal factors involved in these signaling pathways on their protein stability. Firstly, we analyzed the key transcription factors in the ET-signaling pathway ETHYLEN-INSENSITIVE 3 (EIN3). In the absence of ethylene, EIN3 is degraded by the 26S proteasome (Heydlauff et al. 2021; Binder 2020). In the presence of ET, EIN3 is stabilized and promotes ET signaling. We found the stabilization of EIN3:GFP (Volz et al. 2013) driven by the EIN3 promoter after the application of the ET-precursor ACC (Fig. 4B, C), Yet, after mock and LPE treatment we could not detect an EIN3 stabilization, which suggests that applied-LPE exerts minor effects on ET production and signaling. Secondly, we analyzed components of the JA-signaling pathway. The JA-signaling repressor JAZ1 is degraded at high JA levels and inhibits JA-signaling by targeting MYC2 at JA-default levels (Grunewald et al. 2009). We found JAZ1:GFP degradation after LPE and JA administration (Fig. 4A, C) in contrast to the mock treatment. This result suggests that LPE triggers the 26S proteasome-mediated degradation of JAZ1 thereby promoting JA-signaling.
LPE targets the RALF23-FERONIA signaling module
The peptide hormones of the RALF group are involved in a multitude of biological signaling processes (Xiao et al. 2019), e.g., the inhibition of the FERONIA-mediated degradation of MYC2, thereby promoting JA-signaling and response (Guo et al. 2018).
Interestingly, we found a big subclass of RALFs (RALF22, RALF23, RALF24, RALF32, RALF33, RALFL) upregulated at 24 h after LPE application (Fig. 4D, F, G). RALF22 and RALF23 were shown to exert a pivotal function in the inhibition of the FER-mediated MYC2-degradation to favor JA-signaling. By contrast, FER and its interaction partner in RIPK-mediated RPM1-induced immunity, RALF1, are strongly downregulated after LPE administration (Fig. 4D, G). Likewise, several members of the MYC2-targeting JA-signaling inhibitors, JAZs (e.g. JAZ1, JAZ2, JAZ6, JAZ12) show a reduced transcript amount after LPE treatment (Fig. 4D).
Studies have shown that RALF23 inhibits FER function thereby supporting MYC2-mediated JA-response (Guo et al. 2018). The FER-RALF module coordinates cell-wall sensing which in turn affects their subcellular localization in response to various stresses (Herger et al. 2019; Zhao et al. 2018). Thus, we analyzed the localization of RALF23 linked to RFP after LPE, JA, and mock treatment. We found that RALF23 internalizes in intracellular speckles at 1 h after LPE and JA treatment (Fig. 4E). This result suggests the involvement of the FER-RALF23 signaling module in LPE-responding molecular processes.
Distinct ROS levels after LPE treatment in JA-signaling mutants
The elevation of in-situ reactive-oxygen species (ROS) homeostasis and the burst of ROS are among the first steps in the response to a pathogenic threat after the perception of pathogenic signatures (Nakagami et al. 2005). In this regard, the JA-mediated defense response is intimately coupled with changes in ROS homeostasis to oppose a necrotrophic invader (Stenzel et al. 2003). We previously showed that LPE increases the levels of in-situ hydrogen-peroxide H2O2 levels which are accompanied by the strong induction of H2O2 inducible gene expression (Volz et al. 2021).
Here, we analyzed whether the distinct defense responses of WT, coi1-21, and fer-4 following LPE pretreatment can be traced back to differences in their adaptive H2O2-homeostasis, by performing 3,3-diaminobenzidine (DAB) histochemical staining. At 24 h after LPE application, the ROS levels in WT and in particular in fer-4 are significantly increased compared to the mock-treated counterpart (Fig. 5A, B). However, we could not detect changes in the ROS accumulation in coi1 after LPE treatment thereby suggesting that the JA-signaling pathway contributes to the LPE-triggered changes in ROS homeostasis.
Discussion
The use of bioactive molecules to modify the plant defense response is an important area of study. However, the role of cell membrane lipids in plant immunity is not well understood. We discovered that the cell membrane-localized phospholipid LPE promotes defense-associated gene expression and primes the plant immune system. The plant cell wall is one of the major carbon sources for necrotrophic pathogens. To penetrate these barriers and assimilate further nutrients from plant cells, pathogens devour their hosts by secreting cutinases and other cell wall degrading enzymes. Lipid priming is a process by which certain lipids, such as LPE, or lipid-derived molecules act as signaling molecules, preparing the plant's defense system to mount a faster and stronger response against potential threats, such as pathogen attacks. This phenomenon is an essential part of the plant’s immune response and is considered a critical aspect of plant defense. One of the well-known lipid signaling molecules involved in lipid priming is jasmonic acid, which is an oxylipin derived from the oxidation of fatty acids (Wasternack 2007; Wasternack & Strnad 2016). Oxylipins are produced when plants are subjected to various biotic (pathogens, insects) and abiotic stresses (mechanical damage, environmental factors). Oxylipins play a crucial role in signaling and regulating defense responses, including the activation of defense-related genes, synthesis of antimicrobial compounds, and the reinforcement of the plant cell wall to prevent pathogen ingress. Phytopathogens often have to overcome these lipid-mediated defense responses to establish successful infections. Researchers study the mechanisms behind lipid priming and how different lipids interact with the plant's defense signaling pathways to gain insights into developing more robust and sustainable methods for crop protection.
LPE application increases resistance to the necrotrophs B. cinerea and C. heterostrophus in Arabidopsis but this effect is eradicated in the coi1 and amplified in the fer-4 mutant. LPE promotes JA-signaling by degrading JAZ1, and ROS-homeostasis is necessary for LPE-mediated immunity. LPE might also repress FER-signaling by promoting the RALF function. Our findings suggest that LPE triggers immunity-associated signatures via affecting the FERONIA-COI1 mediated JA-signaling and ROS-homeostasis.
The concept of lipid priming has promising applications in agriculture. By priming crop plants with jasmonic acid or its analogs, farmers may improve crop resistance to pathogens and pests, reducing the reliance on chemical pesticides and promoting sustainable agriculture practices. Modifying in-situ LPE homeostasis in crops via the genomic engineering of LPE homeostasis might give rise to the improvement of crop stress resistance. To challenge this concept, we analyzed mutants that are affected by the biosynthesis and catabolism of LPE. The disruption of LPEAT1 and LPEAT2 results in an increased in-situ level of LPE. Vice versa, we analyzed the phospholipase 2alpha mutant (pla2alpha) which is deficient in its level and production of LPE. After B. cinerea infection, we detected reduced lesion formation and increased resistance in lpeat1 and lpeat2. By contrast, pla2alpha exhibited stronger lesion formation and reduced resistance. In summary, we revealed that increased in-situ LPE levels exert an immunity-promoting effect in accordance with the exogenous LPE application. Importantly, these studies suggest that the genomic engineering of LPE levels in crops (e.g. rice) might be advisable for further research. We anticipate that crops with elevated LPE-homeostasis might be tolerant to facing biotic threats and abiotic stresses in a hostile environment. Thus, it might be advisable to generate rice lines, deficient in LPEAT1 and LPEAT2 with increased in-situ LPE levels, and to study their resistance to biotic threats, such as B. cinerea, Magnaporthe oryzae, and C. heterostrophus.
Materials and methods
Plant material and growth conditions
The A. thaliana ecotypes Columbia (Col-0) and Wassilewskija (Ws-0) used in this study were obtained from the Arabidopsis Biological Resource Center (ABRC, Ohio State University). In addition to these ecotypes, we used the following mutants and marker lines derived from Col-0:, coi1-21 N68754, coi11-22, fer-4 N69044, lpeat1 GK-825D08, lpeat2 SALK_107699C, pla2-aplha SALK_099415C, COI1ox N2105631,. p35S::JAZ1:GFP N799800 were ordered by NASC.
Arabidopsis thaliana plants were grown on a mixture of commercial potting soil and perlite (3:1) or on Murashige and Skoog (MS) agar medium) in a growth chamber with 16/8 h day/night photoperiod, 22 °C and 80% relative humidity.
LPE-pretreatment followed by infection experiments
Four- to five-week-old A. thaliana plants, which were grown in soil and irrigated from the bottom, were treated by spraying them with either a mock solution of 250 ppm Tween 80 or a solution of 50 ppm LPE (50 mg/l) in 250 ppm Tween 80, 24 h prior to inoculation with the pathogen. The plants were then placed under a hood and maintained at a temperature of 22 °C in the growth chamber.
Botrytis cinerea spores were prepared for each experiment by using a spore stock stored at − 80 °C in 25% glycerol. The spores were spread evenly on a PDA medium and incubated at 24 °C for 14 days. After harvesting, the spore concentration was determined using a hemocytometer. Leaves were inoculated with a 4 µl droplet of a spore suspension (5 × 105 spores/ml) and analyzed after 72 h post-infection (hpi). At least 30 plants of each plant genotype were sampled in three biological replicates.
Cochliobolus. heterostrophus (C4—Tox1+;MAT-2) conidia were grown on sucrose-proline agar at 22℃. Inoculation of 4-week-old A. thaliana plants with C. heterostrophus involved harvesting conidia from fungal cultures on sucrose-proline agar, and adjusting their concentration to 5 × 105 conidia/ml with water. A total of 20 ml of the conidial suspension was sprayed on five plants using an air paintbrush connected to a compressor. The inoculated plants were incubated in a dew chamber for 16 h at 25 °C under 100% relative humidity and then transferred to a growth chamber at 22 °C and 80% relative humidity to observe disease development. Each inoculation experiment was repeated three times. To assess disease severity, a numerical scoring system based on the percentage of diseased leaf area (DLA) was used. ImageJ software was used to determine DLA based on brightness intensity values at 3 and 6 days post-inoculation (dpi). DLA scores ranged from 0 to 5, with 0 indicating no necrotic or chlorotic flecks on the leaves (water controls always received a score of 0). The numerical values corresponded to the percentage of leaf area showing necrosis or chlorosis, with 1 representing 1–20%, 2 representing 21–40%, 3 representing 41–60%, 4 representing 61–80%, and 5 representing 81–100%.
Immunoblot
Nuclear proteins were extracted from 14-day-old seedlings grown on half MS-medium. The proteins were quantified using the Bradford method, and equal amounts of proteins were separated by SDS-PAGE. The separated proteins were then transferred to a polyvinylidene difluoride membrane (Bio-Rad) using a Mini-Protean 3 Cell (Bio-Rad). Immunoblot analysis was carried out using primary polyclonal pTpY antibodies at a concentration of 1 µg/mL, followed by secondary antibodies conjugated to alkaline phosphatase. Chemiluminescence was used to detect the antibody complexes using the Immun-Start AP Substrate kit (Bio-Rad). The experiment was repeated three times, and a representative result from one bioRep is shown.
Quantitation of immunoblot membranes
To measure protein levels in extracts and guarantee uniform loading of all proteins for gels used in immunoblot analysis, Bradford assays were utilized.
Molecular cloning
The coding sequence of RALF23 was amplified form an Arabidopsis seedling cDNA pool without an STOP codon and cloned into the Gateway-based pENTR-D-Topo vector. After sequencing the RALF23 cds, it was cloned into the pDEST::C-RFP-Stop plasmid to generate pDEST::RALF23:RFP. Subsequently, pDEST::RALF23:RFP was used to transform Arabidopsis Col plants by employing the Agrobacterium-mediated transformation.
Histochemical staining and ROS-burst assay
Histochemical staining using 3,3′-diaminobenzidine (DAB) was used to perform in situ detection of H2O2 in 4-week-old A. thaliana plants treated with LPE (50 mg/l) or mock solution, following a previously described protocol. In brief, leaves were incubated in DAB solution (50 mg DAB, 130 mg Na2HPO4, 0.01%v/v Tween 20) for 6 h. Subsequently, the leaves were mounted in 20% glycerol. This experiment was repeated three times, and a representative result is shown.
RNA-extraction, cDNA synthesis and qPCR
To analyze the expression levels of individual marker genes, RT-PCR was conducted on plants grown on plates containing half-strength Murashige and Skoog (MS), 0.5% sucrose, 1% agar, and 0.5% MES at pH 5.7. Total RNA was extracted from LPE (50 mg/l) and mock-treated 14-day-old seedlings using the easy-spinTM Total RNA Extraction Kit. Reverse transcription was performed using SuperScript II reverse transcriptase, and RT-qPCR was carried out using an Applied Biosystems 7500 Real-Time PCR systemTM and an SYBR green PCR master mix. Data were averaged from duplicates of at least three biological replicates, and oligo-nucleotides used to determine transcript levels.
RV4s | GAATCGGTCGTTTGGTTGCTA | qPCR GADPH |
---|---|---|
RV4as | TTAACAGCGACGAGCTCAACAT | qPCR GADPH |
RV15s | GTACCGCCGTCTAAGGACAT | qPCR AOC2 |
RV15as | CACAGCGATACGAGAAACATT | qPCR AOC2 |
92 s | CGTTGTTCAAGCACCAGAGA | qPCR MEE14, CBP1 |
92as | CGGATTCGGCTTTTTCAATA | qPCR MEE14, CBP1 |
93 s | CGTACGTGAATGGCAAGCTA | qPCR NAD(P) BP AT2G29170 |
93as | GGCAACGTTTTCCATGAGTT | qPCR NAD(P) BP AT2G29170 |
94 s | CGTGGCTCGTGTGATAAAGA | qPCR SAG29 |
94as | CGGCGCTTATAGTGAGGAAG | qPCR SAG29 |
95 s | TGCTTACGTTGGACAGCTTG | qPCR NPF6 |
95as | ACTCACGAAGAATCCCATCG | qPCR NPF6.4 |
96 s | CGGCTTTTGCTTAGTTCCTG | qPCR COL7 CONSTANS-LIKE 7 |
96as | CCATGATTGCCTTCTCGACT | qPCR COL7 CONSTANS-LIKE 7 |
165 s | TCGCCGTATCTTCTCAATCC | qPCR RALF23_ |
165as | CAGAGCACTCGGCAATTGTA | qPCR RALF23_ |
166 s | TCTCCGTCACAGTTCCGATT | qPCR RALF24_ |
166as | ATCTCCGATGGCATCATCTC | qPCR RALF24_ |
167 s | AACAGTGTGCCTTGTTCACG | qPCR_RALF22_ |
167as | CTGTAAGGATTCGCCTGAGC | qPCR_RALF22_ |
168 s | TCTTGGTCAAGCCAGAGGTT | qPCR_RALF32_ |
168as | AAGACTCGCCTCGTTTACCA | qPCR_RALF32_ |
169 s | ACTCTCCACAAAACCCGTTG | qPCR_RALF33_ |
169as | CGACTCGATCGGTACGAAAT | qPCR_RALF33_ |
173 s | GGCGTTGTTCTATTCGAAGC | qPCR Feronia |
173as | TGTTCCTTTGCAAGTGTTGG | qPCR Feronia |
174 s | CGACTGGAGACAATGGTTCA | qPCR RALF1 |
174as | TTGTGGTCGCCAATATTCTTC | qPCR RALF1 |
182 | ACGGTCTCAACGCTACCAAC | fer-4 genotyping |
183 | TTTCCCGCCTTCGGTTTA | T-DNA of fer4 |
184 s | GATTACTCTCCAACAGAGAAAATCCT | fer-4 genotyping |
184as | CGTATTGCTTTTCGATTTCCTA | fer-4 genotyping |
185 s | CTG TAA GCA GTT GAA GCG GCT GAG GAT TGA A | coi1-22 |
185as | GTC TCA GAT AGA ATG CAA ATC GTC TGA GTT TCT TGG AT | coi1-22 BamH1 |
186 s | GAC AAC ACT TGT TGT TTT TCT TCA GAC AAG GAA TGT AAC CG | coi1-21 |
186as | GGT CGA GTA AGA CAA GGC GGA AGT CAC AGA GGT T | coi1-21 HpaII |
232as | TTATCACCGACCATTGTTGCATCG | lpeat2 mutant, SALK_107699C |
233as | TGCTCCAACTATTATGCTTTTTCCAG | lpeat1 mutant, GABI_825D08 |
234 | CCATATTGACCATCATACTCATTGC | Left-Border for T-DNA (Gabi-Kat; pAC161) |
235 | CAAGTGGATTGATGTGATATCTCC | Right-Border for T-DNA (Gabi-Kat; pAC161) |
236 s | AAAGTCATGTAGCCTAACACGTC | lpeat2 mutant, bind on 9.exon, with 232as |
237 s | TTTGACTAAGATTACCATTGAGAG | lpeat1 mutant, bind on 9.exon, with 233as |
238 s | tcgatatacagaagttctttgagc | T-DNA line for ATSPLA2-ALPHA, AT2G06925 |
238as | ttagccatacgaaacaaatgagtc | T-DNA line for ATSPLA2-ALPHA, AT2G06925 |
RNA-sequencing and bioinformatics analysis
The RNA sequencing was performed using Illumina sequencer (NICEM, Seoul, Korea). The quality of the raw sequence reads was checked using FastQC v0.11.9 (Andrews 2017), and the adaptors and poor reads were removed by Fastp v0.20.1 (Chen et al. 2018). The quality-checked reads were mapped to the reference genome TAIR10 using HISAT2 v2.1.0 aligner (Kim et al. 2019). The mapped reads were sorted using SAMsort in Picard v2.23.3 (Broad Institute). Read counting was performed using StringTie v2.1.3 (Pertea et al. 2015), and differentially expressed gene analysis was executed by DESeq2 (Love et al. 2014). The gene ontology enrichment test was performed with TAIR10 data in AgriGO v2.0 (Tian et al. 2017). Three biological replicates for mock, 6 h and 24 h were analyzed.
Statistical analysis
Statistical significance was determined using one-way ANOVA with Tukey post-test. The significance level was set at p ≤ 0.05, and different letters above the bars indicate significant differences. Samples sharing letters are not significantly different. Asterisks were used to indicate the level of significance, with n.s. representing non-significant results, *indicating p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001.
Data availability
All relevant data can be found within the manuscript and in its Supporting Information online at the publisher’s website.
References
Amaro AL, Almeida DPF (2013) Lysophosphatidylethanolamine effects on horticultural commodities: a review. Postharvest Biol Technol 78:92–102
Andrews S, 2017. FastQC: a quality control tool for high throughput sequence data. 2010. In.
Binder BM (2020) Ethylene signaling in plants. J Biol Chem 295:7710–7725
Canonne J, Froidure-Nicolas S, Rivas S (2011) Phospholipases in action during plant defense signaling. Plant Signal Behav 6:13–18
Chen S, Zhou Y, Chen Y, Gu J (2018) Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890
Escobar-Restrepo JM, Huck N, Kessler S et al (2007) The FERONIA receptor-like kinase mediates male-female interactions during pollen tube reception. Science 317:656–660
Farag KM, Palta JP (1993) Use of Lysophosphatidylethanolamine, a Natural Lipid, to Retard Tomato Leaf and Fruit Senescence. Physiol Plant 87:515–521
Grunewald W, Vanholme B, Pauwels L et al (2009) Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin. EMBO Rep 10:923–928
Guo H, Nolan TM, Song G et al (2018) FERONIA receptor kinase contributes to plant immunity by suppressing jasmonic acid signaling in Arabidopsis thaliana. Curr Biol 28(3316–24):e6
He Y, Chung EH, Hubert DA, Tornero P, Dangl JL (2012) Specific missense alleles of the arabidopsis jasmonic acid co-receptor COI1 regulate innate immune receptor accumulation and function. PLoS Genet 8:e1003018
Herger A, Dunser K, Kleine-Vehn J, Ringli C (2019) Leucine-rich repeat extensin proteins and their role in cell wall sensing. Curr Biol 29:R851–R858
Heydlauff J, Serbes IE, Vo D et al (2021) Dual and opposing roles of EIN3 reveal a generation conflict during seed growth. Mol Plant 15(2):363–371
Hong JH, Chung G, Cowan AK (2009) Delayed leaf senescence by exogenous lyso-phosphatidylethanolamine: towards a mechanism of action. Plant Physiol Biochem 47:526–534
Jasieniecka-Gazarkiewicz K, Lager I, Carlsson AS et al (2017) Acyl-CoA: Lysophosphatidylethanolamine Acyltransferase activity regulates growth of Arabidopsis. Plant Physiol 174:986–998
Kim D, Paggi JM, Park C, Bennett C, Salzberg SL (2019) Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat Biotechnol 37:907–915
Lee HY, Bahn SC, Shin JS et al (2005) Multiple forms of secretory phospholipase A2 in plants. Prog Lipid Res 44:52–67
Lee OR, Kim SJ, Kim HJ et al (2010) Phospholipase A(2) is required for PIN-FORMED protein trafficking to the plasma membrane in the Arabidopsis root. Plant Cell 22:1812–1825
Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:550
Marin-De La Rosa N, Pfeiffer A, Hill K et al (2015) Genome wide binding site analysis reveals transcriptional Coactivation of Cytokinin-responsive genes by DELLA proteins. PLoS GEnet 11:e1005337
Meng X, Zhang S (2013) MAPK cascades in plant disease resistance signaling. Annu Rev Phytopathol 51:245–266
Mizoi J, Nakamura M, Nishida I (2006) Defects in CTP: Phosphorylethanolamine cytidylyltransferase affect embryonic and postembryonic development in Arabidopsis. Plant Cell 18:3370–3385
Nakagami H, Pitzschke A, Hirt H (2005) Emerging MAP kinase pathways in plant stress signalling. Trends Plant Sci 10:339–346
Ozgen M, Serce S, Akca Y, Hong JH (2015) Lysophosphatidylethanolamine (LPE) improves fruit size, color, quality and phytochemical contents of sweet cherry c.v. “0900 Ziraat.” Korean J Horticult Sci Technol 33:196–201
Pertea M, Pertea GM, Antonescu CM, Chang TC, Mendell JT, Salzberg SL (2015) StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol 33:290–295
Ryu SB, Karlsson BH, Ozgen M, Palta JP (1997) Inhibition of phospholipase D by lysophosphatidylethanolamine, a lipid-derived senescence retardant. Proc Natl Acad Sci U S A 94:12717–12721
Stenzel I, Hause B, Miersch O et al (2003) Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana. Plant Mol Biol 51:895–911
Tian T, Liu Y, Yan H et al (2017) agriGO v2. 0: a GO analysis toolkit for the agricultural community, 2017 update. Nucleic Acids Res 45:W122–W129
Volz R, Heydlauff J, Ripper D, Von Lyncker L, Gross-Hardt R (2013) Ethylene signaling is required for synergid degeneration and the establishment of a pollen tube block. Dev Cell 25:310–316
Volz R, Park JY, Kim S et al (2020) The rice/maize pathogen Cochliobolus spp. infect and reproduce on Arabidopsis revealing differences in defensive phytohormone function between monocots and dicots. Plant J 103:412–429
Volz R, Park JY, Harris W, Hwang S, Lee YH (2021) Lyso-phosphatidylethanolamine primes the plant immune system and promotes basal resistance against hemibiotrophic pathogens. BMC Biotechnol 21:12
Wang X (2005) Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses. Plant Physiol 139:566–573
Wasternack C (2007) Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot 100:681–697
Wasternack C, Strnad M (2016) Jasmonate signaling in plant stress responses and development - active and inactive compounds. N Biotechnol 33:604–613
Xiao Y, Stegmann M, Han Z et al (2019) Mechanisms of RALF peptide perception by a heterotypic receptor complex. Nature 572:270–274
Zhao C, Zayed O, Yu Z et al (2018) Leucine-rich repeat extensin proteins regulate plant salt tolerance in Arabidopsis. Proc Natl Acad Sci U S A 115:13123–13128
Zhou Y, Zhou DM, Yu WW et al (2022) Phosphatidic acid modulates MPK3- and MPK6-mediated hypoxia signaling in Arabidopsis. Plant Cell 34:889–909
Funding
This work was supported by the National Research Foundation of Korea, Grant/Award Number: 2020R1A2B5B03096402, 2018R1A5A1023599, and 2021M3H9A1096935. W. Harris is grateful for a graduate fellowship from the Brain Korea 21 Plus Program.
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RV and YHL: designed, conceived and analyzed the experiments and results. K-TK: conducted the bioinformatic analysis of the RNAseq-data. MA and WH: carried out immunoblot, protein quantification and pathogen-treatment. RV wrote the original draft. MA, WH, K-TK and YHL: edited the manuscript. All authors read and approved the final manuscript.
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11103_2023_1385_MOESM1_ESM.tif
Supplementary file1 (TIF 25517 KB) Fig S1: Differentially-regulated genes after LPE-application. (A) Number of up and downregulated genes in the LPE-transcriptome study. (B) Upregulated defense-associated pathways at 6 hrs followed by a predominate downregulation at 24 hrs. (C) Differentially-regulated genes contribute to the JA-biosynthesis (AOC1, AOC2, AOS, JOX4) and JA-signaling pathway (ORA59, PDF1.2, JAZ6, PR4, and WAX1) Predominately downregulated at 6 hrs and upregulated after 24 hrs following LPE application are associated to small molecular metabolic and nucleotide processes, and response to light intensity and photosynthesis. (D) Expression study of PDF1.2 and ORA59 6 hours after mock and LPE-application.
11103_2023_1385_MOESM2_ESM.xlsx
Supplementary file2 (XLSX 450 KB) Table S1: Differentially regulated genes (up and downregulated) 6 hours after LPE application, p<0.01
11103_2023_1385_MOESM3_ESM.xlsx
Supplementary file3 (XLSX 677 KB) Table S2: Differentially regulated genes (up and downregulated) 24 hours after LPE application, p<0.01
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Vӧlz, R., Kim, KT., Alazem, M. et al. Lyso-phosphatidylethanolamine triggers immunity against necrotrophs by promoting JA-signaling and ROS-homeostasis. Plant Mol Biol 113, 237–247 (2023). https://doi.org/10.1007/s11103-023-01385-x
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DOI: https://doi.org/10.1007/s11103-023-01385-x