Abstract
Uridine diphosphate-glucose dehydrogenase (UGD, EC1.1.1.22 oxidizes UDP-Glc (UDP-D-glucose) to UDP-GlcA (UDP-Dglucuronate), a critical precursor of cell wall polysaccharides. GbUGD6 from Gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (DPA)) and during the stage of secondary cell wall thickening (30 DPA). Subcellular localization analysis in onion epidermis revealed that fluorescently labeled GbUGD6 protein was distributed throughout the cell membrane, as well as the nucleus and vacuoles. Examination of UGD function in Arabidopsis revealed that the antisense GbUGD6 lines had shorter roots, deferred blossoming, compared to wild-type plants. Activities of associated enzymes were also affected by UGD reduction, and biochemical analysis of cell wall samples showed an increase in cellulose levels and a decrease in UGP-GlcA contents. The results of the present study as well as previous studies on UGD support the conclusion that UGD plays a major role in synthesizing polysaccharides synthesis in the cell wall.
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Bindschedler, L.V., Wheatley, E., Gay, E., Cole, J., Cottage, A., and Bolwell, G.P. (2005). Characterisation and expression of the pathway from UDP-glucose to UDP-xylose in differentiating tobacco tissue. Plant Mol Biol 57, 285–301.
Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16, 735–743.
Diaz-De-Leon, F., Klotz, K.L., and Lagrimini, L.M. (1993). Nucleotide sequence of the tobacco (Nicotiana tabacum) anionic peroxidase gene. Plant Physiol 101, 1117–1118.
Diet, A., Link, B., Seifert, G.J., Schellenberg, B., Wagner, U., Pauly, M., Reiter, W.D., and Ringli, C. (2006). The Arabidopsis root hair cell wall formation mutant lrx1 is suppressed by mutations in the RHM1 gene encoding a UDP-L-rhamnose synthase. Plant cell 18, 1630–1641.
Johansson, H., Sterky, F., Amini, B., Lundeberg, J., and Kleczkowski, L.A. (2002). Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation. Biochim Biophys Acta 1576, 53–58.
Karkonen, A., Murigneux, A., Martinant, J.P., Pepey, E., Tatout, C., Dudley, B.J., and Fry, S.C. (2005). UDP-glucose dehydrogenases of maize: a role in cell wall pentose biosynthesis. Biochem J 391, 409–415.
Klinghammer, M., and Tenhaken, R. (2007). Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls. J Exp Botany 58, 3609–3621.
Kuhn, J.H., Becker, S., Ebihara, H., Geisbert, T.W., Johnson, K.M., Kawaoka, Y., Lipkin, W.I., Negredo, A.I., Netesov, S.V., Nichol, S.T., Palacios, G., Peters, C.J., Tenorio, A., Volchkov, V.E., and Jahrling, P.B. (2010). Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations. Arch Virol 155, 2083–2103.
Li, N., Wang, L., Zhang, W., Takechi, K., Takano, H., and Lin, X. (2014). Overexpression of UDP-glucose pyrophosphorylase from Larix gmelinii enhances vegetative growth in transgenic Arabidopsis thaliana. Plant Cell Rep 33, 779–791.
Malinova, I., Kunz, H.H., Alseekh, S., Herbst, K., Fernie, A.R., Gierth, M., and Fettke, J. (2014). Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning. PLoS One 9, e112468.
Naoumkina, M., Hinchliffe, D.J., Turley, R.B., Bland, J.M., and Fang, D.D. (2013). Integrated metabolomics and genomics analysis provides new insights into the fiber elongation process in Ligon lintless-2 mutant cotton (Gossypium hirsutum L.). BMC Genomics 14, 155.
Padmalatha, K.V., Patil, D.P., Kumar, K., Dhandapani, G., Kanakachari, M., Phanindra, M.L., Kumar, S., Mohan, T.C., Jain, N., Prakash, A.H., Vamadevaiah, H., Katageri, I.S., Leelavathi, S., Reddy, M.K., Kumar, P.A., and Reddy, V.S. (2012). Functional genomics of fuzzless-lintless mutant of Gossypium hirsutum L. cv. MCU5 reveal key genes and pathways involved in cotton fibre initiation and elongation. BMC Genomics 13, 624.
Pan, Y., Ma, J., Zhang, G., Han, G., Wang, X., and Ma, Z. (2007). cDNA-AFLP profiling for the fiber development stage of secondary cell wall synthesis and transcriptome mapping in cotton. Chin Sci Bull 52, 2358–2364.
Pang, C., Wang, H., Pang, Y., Xu, C., Jiao, Y., Qin, Y., Western, T.L., Yu, S., and Zhu, Y. (2010). Comparative proteomics indicates that biosynthesis of pectic precursors is important for cotton fiber and Arabidopsis root hair elongation. Mol Cell Proteomics 9, 2019–2033.
Reboul, R., Geserick, C., Pabst, M., Frey, B., Wittmann, D., Lutz-Meindl, U., Leonard, R., and Tenhaken, R. (2011). Down-regulation of UDP-glucuronic acid biosynthesis leads to swollen plant cell walls and severe developmental defects associated with changes in pectic polysaccharides. J Biol Chem 286, 39982–39992.
Robertson, D., Smith, C., and Bolwell, G.P. (1996). Inducible UDP-glucose dehydrogenase from French bean (Phaseolus vulgaris L.) locates to vascular tissue and has alcohol dehydrogenase activity. Biochem J 313, 311–317.
Seifert, G.J. (2004). Nucleotide sugar interconversions and cell wall biosynthesis: how to bring the inside to the outside. Curr Opin Plant Biol 7, 277–284.
Tenhaken, R., and Thulke, O. (1996). Cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: UDP-glucose dehydrogenase. Plant Physiol 112, 1127–1134.
Updegraff, D.M. (1969). Semimicro determination of cellulose in biological materials. Anal Biochem 32, 420–424.
Wang, Q., Zhang, X., Li, F., Hou, Y., Liu, X., and Zhang, X. (2011). Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Plant Cell Rep 30, 1303–1312.
Zablackis, E., Huang, J., Muller, B., Darvill, A.G., and Albersheim, P. (1995). Characterization of the cell-wall polysaccharides of Arabidopsis thaliana leaves. Plant Physiol 107, 1129–1138.
Cao, X. (2015). Whole genome sequencing of cottona new chapter in cotton genomics. Sci China Life Sci 58, 515–516.
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Han, J., Pan, Y., Wang, X. et al. Antisense expression of Gossypium barbadense UGD6 in Arabidopsis thaliana significantly alters cell wall composition. Sci. China Life Sci. 59, 213–218 (2016). https://doi.org/10.1007/s11427-016-5004-y
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DOI: https://doi.org/10.1007/s11427-016-5004-y