Abstract
In Parkinson’s disease, the dysfunction of the dopaminergic nigrostriatal tract involves the loss of function of dopaminergic neurons of the substantia nigra pars compacta followed by death of these neurons. The functional recovery of these neurons requires a deep knowledge of the molecules that maintain the dopaminergic phenotype during adulthood and the mechanisms that subvert their activity. Previous studies have shown that transcription factor NURR1, involved in differentiation and maintenance of the dopaminergic phenotype, is downregulated by α-synuclein (α-SYN). In this study, we provide a mechanistic explanation to this finding by connecting α-SYN-induced activation of glycogen synthase kinase-3 (GSK-3) with NURR1 phosphorylation followed by proteasomal degradation. The use of sequential deletion mutants and single point mutants of NURR1 allowed the identification of a domain comprising amino acids 123-PSSPPTPSTPS-134 that is targeted by GSK-3 and leads to subsequent ubiquitination and proteasome degradation. This study provides a detailed analysis of the regulation of NURR1 stability by phosphorylation in synucleinopathies such as Parkinson’s disease.
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Introduction
Midbrain dopaminergic (DAergic) neurons are the main source of dopamine (DA) in the mammalian central nervous system. Several transcription factors have been implicated in DAergic differentiation [1]. Among them, nuclear receptor‐related factor 1 (NURR1; also known as NR4A2) is a transcription factor of the orphan nuclear receptor class that participates in acquisition of the DAergic phenotype in neurons during development and in the maintenance of their functionality during adulthood [2,3,4]. It regulates the expression of several genes involved in DA metabolism, including tyrosine hydroxylase (TH) [5,6,7], dopamine transporter (DAT) [8], amino acid decarboxylase (AADC) [9], vesicular monoamine transporter-2 (VMAT2) [9], as well as other non-DAergic genes such as NRP1 [10] and RET (GDNF receptor) [11]. Ablation of NURR1 in adult rodents results in reduced expression of its target genes and loss of functional DAergic midbrain neurons [12,13,14]. Mutations in the human NURR1 gene have been identified in association with Parkinson’s disease (PD), where neurodegeneration of the DAergic neurons of the SN occurs [15, 16].
DAergic neuronal loss is associated with abnormal accumulation and aggregation of the protein α-synuclein (α-SYN) in the form of Lewy bodies and Lewy neurites [17]. Point mutations, duplications, and triplications of α-SYN gene (SNCA) are associated with familiar forms of PD [18, 19], which indicate a key role of this protein in the neurodegenerative process of the disease. The toxicity elicited by α-SYN oligomers correlates with its phosphorylation at serine 129 as this event promotes fibril formation [20, 21]. Related to NURR1, a seminal study, demonstrated that aberrant expression of human α-SYN in murine DAergic neurons correlates with exacerbated proteasomal degradation of NURR1 and loss of the DAergic phenotype [22]. However, little is known about the molecular mechanisms that connect synucleinopathy with loss of NURR1 stability and loss of DAergic neuron functionality, which may be more important than frank cell loss [3, 12]. This information is crucial to identify early signs of damaged DAergic neurons and to apply a neuroprotective therapy before the manifestation of DAergic cell death.
Phosphorylation is a mechanism used in many proteins to target their proteolytic degradation. For instance, glycogen synthase kinase 3 (GSK-3) phosphorylates several proteins to create a phosphorylation-dependent degradation domain (phosphodegron) that is then recognized by a variety of E3 ubiquitin ligase adapters leading to proteasomal degradation of the phosphorylated protein [23]. Considering that GSK-3 is activated by α-SYN aggregates [24,25,26,27,28], here, we analyzed if NURR1 is phosphorylated by GSK-3 and sent to ubiquitin proteasome degradation phosphorylation by GSK-3. The two isoforms of GSK-3 (GSK-3α and GSK-3β) play critical roles in metabolism, neurogenesis, proliferation, neuronal differentiation, and neuronal death [29], and their dysregulation is associated with neurodegenerative diseases. For instance, abnormal GSK-3β activity leading to TAU phosphorylation and aggregation has been extensively reported in Alzheimer’s disease [30,31,32] but also in connection with several hallmarks of PD [33,34,35,36]. Thus, in postmortem PD brain samples, GSK-3β activity is increased in regions related to PD pathology, and GSK-3β co-localizes with α-SYN in Lewy bodies [35, 36]. α-SYN pathology leads to GSK-3β activation, subsequent phosphorylation of several transcription factors such as Jun, Myc, HSF-1, and CREB, and neuronal death, thus opening the possibility of a similar regulation of NURR1 [28].
In this study, using several models of synucleinopathy, we found that α-SYN-induced activation of GSK-3β leads to phosphorylation of NURR1 and its subsequent ubiquitin–proteasome degradation, which precedes loss of the DAergic phenotype.
Materials and Methods
A detailed description of methods is presented in Supplemental Material
Cell Culture and Reagents
Human embryonic kidney 293 T with SV40 T antigen (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, Madrid, Spain) supplemented with 10% fetal bovine serum (Invitrogen, CA, USA) and 80 µg/ml gentamycin (Gibco, MA, USA). Human neuroblastoma cells (SH-SY5Y) were cultured in RPMI supplemented with 10% fetal bovine serum (Invitrogen) and 80 µg/ml gentamicin. The SH-SY5Y-α-SYN Tet-Off cells, described previously [37, 38], were cultured in RPMI with 10% fetal bovine serum, 250 µg/ml G418 (Gibco), 50 µg/ml hygromycin B (Invitrogen), and 2 µg/ml doxycycline (DOX) (Sigma-Aldrich). The expression of α-SYN was switched on by DOX removal. Transient transfection of HEK293T cells was performed with TransFectin Lipid Reagent (Bio-RAD, CA, USA). The inhibitors SB216763, LY294002, and MG132 were from Sigma-Aldrich. Cycloheximide (CHX) was from Boehringer Mannheim (Stuttgart, Germany).
Plasmids and Lentiviruses
The vectors pCGN-HA-GSK-3βΔ9, pCGN-HA-GSK-3βWT, and pCGN-HA-GSK-3βY216F were provided by Dr. Akira Kikuchi (Department of Biochemistry, Faculty of Medicine, Hiroshima University). Vectors pGL3-NBRE3xLuc and pGL3-TkLuc were provided by Dr. Philippe Lefebvre (INSERM Institut Pasteur de Lille, Lille, France). The HA-Ubiquitin expression vector was provided by Dr. Tadashi Nakagawa (Division of Cell Proliferation, ART, Tohoku University Graduate School of Medicine, Sendai, Japan). The plasmid pcDNA3.1-Nurr1WT-V5/6xHis has been described previously [39]. The lentiviral particles used in this study were purchased from Addgene and were generated in HEK293T cells as described previously ([40] and Supplemental Material).
α-SYN Pre-formed Fibrils (PFF)
Purified monomeric α‐SYN was purchased from Proteos, Inc (cat no. RP‐003), and PFFs were formed according to the protocol provided by the manufacturer [41, 42] (see Supplemental Material).
Immunoblotting
This protocol was performed as described in [43]. Briefly, cells were homogenized in lysis buffer (TRIS pH 7.6 50 mM, 400 mM NaCl, 1 mM EDTA, 1 mM EGTA, and 1% SDS), and samples were heated at 95 °C for 15 min, sonicated and pre-cleared by centrifugation. Proteins were resolved in SDS-PAGE and transferred to Immobilon-P (Merck-Millipore, MA, USA) membranes. Proteins of interest were detected with the primary antibodies indicated in the Supplemental Table 2. Proper peroxidase-conjugated secondary antibodies were used for detection by enhanced chemiluminescence (GE Healthcare).
Immunofluorescence
SH-SY5Y cells were seeded in 24-well plates (5 × 103 cells/well) on poly-D-Lys-covered slides and treated with 1 µg/ml PFFs. The protocols have been previously described [39, 44, 45]. Primary antibodies recognized TH (Merck-Millipore), human α-SYN (Santa Cruz Biotechnology, Dallas, TX, USA) and α-SYN-pSer129 (Abcam, Cambridge, UK). Secondary antibodies were as follows: Alexa Fluor 488 donkey anti-mouse, and Alexa 546 donkey anti-rabbit (1:500; Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Fluor 546-conjugated donkey anti-mouse IgG (Molecular Probes, Eugene, OR, USA). Control sections were treated identically but omitting the primary antibody.
In vivo Ubiquitination Assay
HEK293T cells co-transfected with expression plasmids for HA-Ubiquitin (HA-Ub), Nurr1WT-V5/6xHis, or Nurr1MUT2-V5/6xHis with pCGN-HA-GSK-3βΔ9 or pCGN-HA-GSK-3βY216F, using TransFectin Lipid Reagent (Bio-RAD). After 5 h, HEK293T cells were treated for 16 h with 2 μM MG132 (Sigma-Aldrich). Cells were then lysed in a RIPA buffer (150 mM NaCl, 25 mM Tris–HCl, pH 7.5, 1% Nonidet P-40, 1% sodium deoxycholate, 1% Triton-X100, 0.1% SDS, 1 mM phenylmethylsulfonyl fluoride, 1 mM NaF, 1 mM sodium pyrophosphate, 1 mM sodium orthovanadate, 1 g/ml leupeptin). Then, samples were kept for 30 min at 4 °C in a rotating wheel and centrifuged at 13,000 rpm for 10 min. Three microliters of the anti-V5 (Invitrogen) was added per lysate, and after incubation for 2 h at 4 °C in a rotating wheel, gamma-bind Sepharose-protein G was added (Amersham Biosciences), followed by incubation for 1 h at 4 °C. The complexes were harvested by centrifugation and washed three washes with RIPA buffer, resolved in SDS–polyacrylamide gels, and immunobloted. Mouse IgG TrueBlot (eBiosciences) was used as a peroxidase-conjugated secondary antibody (1:10,000 dilution) because it reduces interference by the 55-kDa heavy and 23-kDa light chains of the immunoprecipitation antibody.
Lambda Phosphatase Assay
HEK293T cells were co-transfected with Nurr1WT-V5/6xHis and pCGN-HA-GSK-3βΔ9 or pCGN-HA-GSK-3βY216F using TransFectin Lipid Reagent (Bio-Rad) according to manufacturer recommendations. After 24 h of recovery from transfection, the cells were lysed in 200 μl lysis buffer (137 mM NaCl, 20 mM Tris–HCl, pH 7.5, 1% Nonidet P40, 10% glycerol, 1 μg/ml leupeptin, 1 mM phenylmethylsulfonyl fluoride). Then, the samples were sonicated and precleared by centrifugation, and 50 μl of the sample was incubated with λ-protein phosphatase (Upstate, Millipore) for 4 h at 37 °C. Then, the samples were resolved by SDS-PAGE and immunoblotted.
Two-Dimensional Electrophoresis
HEK293T cell co-transfected with expression plasmids for Nurr1WT-V5/6xHis, Nurr1MUT2-V5/6xHis and pCGN-HA-GSK-3βΔ9 or pCGN-HA-GSK-3βY216F, using TransFectin Lipid Reagent (Bio-RAD) according to manufacturer recommendations. For experimental details, see Supplemental Material.
Analysis of mRNA Levels
Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions (Invitrogen). Reverse transcription and quantitative PCR were done as detailed elsewhere [44]. Primer sequences are shown in Supplemental Table 3. Data analysis was based on the ΔΔCT method with normalization of the raw data to housekeeping genes Actb and Gapdh (Applied Biosystems, Thermo Fisher Scientific). All PCRs were performed in triplicate.
Luciferase Assays
Luciferase activities were determined using a luciferase assay system (Promega) as per the manufacturer’s instructions. As a reference plasmid to normalize transfection efficiency, a CMV-galactosidase plasmid (Promega) was cotransfected in all experiments and luciferase assay values were normalized to galactosidase activity.
Statistics
Results are expressed as mean ± SEM from at least three independent experiments. Data were analyzed by one-way ANOVA followed by Newman–Keuls multiple comparison test (p ≤ 0.001), or with Student’s t test (p ≤ 0.05), using Prism version 5.03 software (GraphPad, San Diego, CA, USA).
Results
α-SYN Aggregates Reduce the DAergic Phenotype of SH-SY5Y Cells
In order to identify the mechanism involved in the dysregulation of the DAergic phenotype, we incubated the DAergic cell line SH-SY5Y with preformed fibrils (PFFs) of human recombinant α-SYN (1 µg/ml, 10 days). Confocal microscopy demonstrated the formation of aggregates containing α-SYN and Ser129-phopshorylated α-SYN (α-SYN-pSer129) (Fig. 1A). PFFs induced a slight nonsignificant decrease in NURR1 transcript levels compared to the control untreated cells, indicating similar NURR1 gene expression, but at the same time, TH and RET transcripts were diminished (Fig. 1B). By contrast, not only TH and RET proteins were decreased but also NURR1 (Fig. 1C and D). The fact that NURR1 gene expression was little or no affected by PFFs (see “Discussion”), together with the decrease in NURR1 protein levels, suggests that α-SYN PFFs must cause, at least in part, a reduction of NURR1 protein stability and subsequent decrease in the expression of NURR1 target genes, such as TH and RET.
We further analyzed the regulation of NURR1 in the Tet-Off SH-SY5Y cell line, conditionally expressing α-SYN in the absence of doxycycline (DOX) [46]. DOX removal from the culture medium led to a robust expression of α-SYN after 8 days, and α-SYN and α-SYN-pSer129 expression correlated with a decrease in NURR1, TH, and RET protein levels (Fig. 2A-C). Moreover, α-SYN overexpression resulted in a reduction of the NURR1 target genes TH and RET (Fig. 2B). To further determine the effect on gene expression, first we transfected these cells with a NBRE-luciferase reporter-construct, specifically activated by NURR1. Upon DOX removal, α-SYN overexpression correlated with reduced luciferase levels (Fig. 2D, left bars), suggesting that NURR1 activity parallels NURR1 protein levels. Similar results were obtained when naïve SH-SY5Y cells were co-transfected with the NBRE-luciferase reporter and expression vectors for wild type or A53T-α-SYN (Fig. 2D, right bars). This effect was not observed upon transfection of the control vector. We further expressed in the Tet-off SH-SY5Y cells, a myc-tagged NURR1 cDNA under the control of a heterologous CMV promoter (Fig. 2E). NURR1 transcript levels arising from the construct were unaffected by α-SYN overexpression, yet TH and RET expression were decreased. These experiments further verify that α-SYN reduces NURR1 protein levels and the expression of its target genes.
GSK-3 Is Needed to Downregulate NURR1
α-SYN overexpression reduced the levels of GSK-3β phosphorylated at Ser9 (GSK-3β-pSer9) (Fig. 2A, B). This phosphoserine exerts an inhibitory effect on the kinase by blocking the catalytic site [47, 48]. Therefore, reduction in pSer9-GSK-3 levels is indicative of increased kinase activity. To determine if increased GSK-3 activity might affect NURR1 stability, we first used the easy-to-transfect human cell line HEK293T. Transcriptomic data indicate that these cells originated from the neural crest and express several neuron-specific genes [49, 50]. We ectopically expressed a V5-targed NURR1 together with constitutively active GSK-3β lacking the first nine amino-terminal residues that correspond to the pseudosubstate (GSK-3βΔ9). Lack of Ser9 renders this kinase insensitive to downregulation by AKT-mediated phosphorylation. As a negative control, we used a hypomorphic mutant containing a single point Tyr-to-Phe mutation in its activation loop (GSK-3βY216F) that renders this kinase almost inactive [51, 52]. As shown in (Fig. 3A), NURR1 levels were reduced with increasing amounts of the GSK-3βΔ9, and were not altered in the presence of inactive GSK-3βY216F. Then, we silenced the expression of both α and β isoforms of this kinase by lentiviral knock-down for 3 days in V5-NURR1 expressing HEK293T cells (Fig. 3B) as well as in naïve SH-SY5Y cells (Fig. 3C). As a control, we analyzed β-catenin levels, a well-established substrate of GSK-3, which is degraded upon GSK-3β-mediated phosphorylation. Just a ~ 50% decrease in GSK-3α and GSK-3β levels increased NURR1 levels and its targets RET and TH. We also silenced both isoforms in the Tet-Off SH-SY5Y-α-SYN cells (Fig. 3D-G). Despite α-SYN overexpression, GSK-3α and GSK-3β knock-down rescued NURR1 levels to baseline as well as TH and RET levels. The effect was most evident in the GSK-3β knocked-down cells, indicating a preponderant role for this isoform. These results show for the first time that GSK-3 is required for the downregulation of NURR1 induced by α-SYN and are in line with observations based on GSK-3 inhibitors in the Parkinsonian MPTP and MPP+ [53, 54], or 6-OHDA [55,56,57] models. The mechanisms of action of these two toxins, altering mitochondrial activity and redox homeostasis, is at least partially different to the proteinopathy elicited by α-SYN aggregates, therefore suggesting that these different pathomechanisms overlap on the GSK-3/NURR1 axis reported here for α-SYN.
GSK-3 Targets NURR1 for Degradation
We next examined the effect of GSK-3 on NURR1 turn-over. SH-SY5Y cells were infected with lentiviral vectors expressing shCTRL, shGSK-3α/shGSK-3β. After 3 days, cells were incubated with the protein synthesis inhibitor cycloheximide (CHX, 100 µM) (Fig. 4A, B). As a control, we monitored the stability of β-catenin. In shCTRL cells, NURR1 had a half-life of ~ 6 h. However, in the GSK-3-knocked-down cells NURR1 was almost completely stable during this time. As an additional approach, we performed similar experiments but inhibiting GSK-3 with the potent and selective inhibitor SB216763 (5 µM, pre-incubated for 2 h) (Fig. 4C, D). In vehicle-treated cells, NURR1 exhibited a half-life of ~ 6 h, as observed before, but in SB216763-treated cells, the levels of NURR1 were hardly affected. Therefore, both genetic and chemical inhibition of GSK-3 results in stabilization of NURR1.
GSK-3β Targets NURR1 for Phosphorylation and Degradation Through the UPS
HEK293T cells were co-transfected with expression vectors for NURR1-V5 and HA-GSK-3βΔ9, or HA-GSK-3βY216F as control. After 16 h, the cells were treated for 2 h and 4 h with the selective proteasome inhibitor MG132 (20 µM). As shown in Fig. 5A, UPS inhibition protected NURR1 from GSK-3β-mediated degradation. In an ubiquitination assay, HEK293T cells were co-transfected with expression vectors for NURR1-V5 along with HA-tagged ubiquitin and either HA-GSK-3βY216F, or HA-GSK-3βWT or HA-GSK-3βΔ9 (Fig. 5B, C). Overexpression of GSK-3βWT slightly ubiquitylated NURR1, and constitutively active GSK-3βΔ9 considerably enhanced ubiquitination. It has been reported previously that NURR1 is degraded by the UPS [22, 58, 59], but our results show for the first time the direct participation of GSK-3β.
GSK-3 phosphorylates its substrates in two specific consensus sequences, (Ser/Thr)-Pro or (Ser/Thr)-X3-(pSer/pThr), where X is any residue [60]. Using the NetPhos 2.0 program, we found that NURR1 contains at least five putative sequences with serines or threonines that conform to the consensus motif for GSK-3 phosphorylation. We named these sites, Core 1, 2, 3, 4, and 5 (Fig. 5D and Supplemental Fig. 1A). Then, we generated sequential deletion mutants fused to enhanced green fluorescence protein (EGFP) at the N-terminus and a V5 tag at the C-terminus (Fig. 5D) and analyzed their phosphorylation pattern (Fig. 5E). GSK-3βΔ9 induced a slightly retarded EGFP-NURR1 band in the full length chimera and in the first deletion mutant (Δ1), compared to control GSK-3βY216F. In addition, the protein levels of these two constructs were decreased in the presence of GSK-3βΔ9. By contrast, the rest of the EGFP-NURR1 mutants exhibited low or no obvious band shift and were resistant to degradation in the presence of GSK-3βΔ9, suggesting that the sites targeted by GSK-3β on NURR1 are preferentially located before or at Core 2 (Fig. 5E). As control, EGFP alone was insensitive to GSK-3β-induced band shift or degradation (data not shown). Additionally, we performed point mutations of NURR1 at Core 2 by changing 4 serines and 2 threonines to alanines (Fig. 5F). We found that Core 2 mutation rendered NURR1 insensitive to GSK-3β-induced band shift and degradation (Fig. 5G). The amino acid sequence of Core 2, comprising residues 123 to 134 is highly conserved in vertebrates (Supplemental Fig. 1B-C).
To confirm that GSK-3β induces NURR1 phosphorylation, we performed a lambda-phosphatase assay (λPPase) in HEK293T cells co-transfected with expression vectors for NURR1-V5 and GSK-3βΔ9 or GSK-3βY216F (Fig. 6A). In the presence of GSK-3βΔ9, NURR1 showed a retarded band in SDS-PAGE. This gel shift was abrogated when the protein lysate was incubated with the phosphatase, therefore demonstrating that the retarded band is due to GSK-3-mediated phosphorylation.
To more precisely characterize the relevance of Core 2, HEK293T cells were transfected with NURR1WT or NURR1MUT2 and co-transfected with GSK-3βΔ9 or GSK-3βY216F, and resolved by 2D gel electrophoresis. As shown in Fig. 6B, NURR1WT co-transfected with hypomorphic GSK-3βY216F, displayed several immunoreactive spots consistent with GSK-3β-independent posttranslational modifications of NURR1. When cells were co-transfected with active GSK-3βΔ9, we observed an increase in the intensity of acidic spots (black arrows), indicating GSK-3-mediated phosphorylation. However, in cells co-transfected with NURR1MUT2 the distribution of spots was similar in the presence of GSK-3βY216F or GSK-3βΔ9, and the acidic shift was not observed (black arrows). These results indicate that the residues of Core 2 are preferred targets of GSK-3β phosphorylation.
In additional experiments, we examined the half-life of NURR1MUT2. HEK293T cells were co-transfected with NURR1WT or NURR1MUT2 and GSK-3βΔ9, and exposed to CHX (100 µM) (Fig. 6C, D). In the presence of GSK-3βΔ9, the half-life of NURR1WT was less than 120 min. However, NURR1MUT2 exhibited a half-life of more than 120 min that was not significantly shortened by the presence of GSK-3βΔ9. We also performed an ubiquitination assay in HEK293T cells co-transfected with expression vectors for NURR1WT or NURR1MUT2 along with HA-Ubiquitin and GSK-3βY216F or GSK-3βΔ9. As shown in Fig. 6E, overexpression of GSK-3βΔ9 enhanced NURR1WT ubiquitination, while in NURR1MUT2 this was less apparent.
Finally, we validated the phosphorylation of Core 2 in the DAergic cell line SH-SY5Y (Fig. 7A). These cells were infected with lentiviral vectors expressing NURR1WT or NURR1MUT2. In the presence of DOX, α-SYN levels were low in cells expressing either form of NURR1, and the levels of GSK-3β-pSer9 were high, indicating its inhibition. However, in the absence of DOX, α-SYN levels increased, and GSK-3 was dephosphorylated and active. The presence of α-SYN and its downstream target, active GSK-3, completely eliminated NURR1WT protein and substantially reduced the levels of TH and RET. By contrast, the levels of NURR1MUT2 were not affected by the α-SYN/GSK-3 challenge and the levels of TH and RET remained similar to those in the absence of α-SYN.
A highly characterized survival pathway in nerve cells is the PI3K/AKT. This pathway is activated by many growth factors and neurotrophins and leads to the inhibition of GSK-3. Therefore, we analyzed the inhibition of this pathway, by using the highly selective PI3K inhibitor LY294002. SH-SY5Y cells were submitted to a time-course of LY294002 (30 µM) alone or in combination with the GSK-3 inhibitor SB216763 (5 μM). As shown in Fig. 7B, C, LY294002 alone led to a decrease in AKT-pSer473 (inactivation) and GSK-3β-pSer9 (activation). Under these conditions, not only β-catenin but also NURR1 levels were gradually decreased. By contrast, cells co-treated with SB216763 were at least partially protected from the decrease in β-catenin and NURR1. Together, these observations confirm in a DAergic cell line the mechanistic connections between α-SYN, GSK-3β, phosphorylation, and degradation of NURR1.
Discussion
The dysfunction of the DAergic nigrostriatal tract in PD proceeds in two phases: first, loss of the DAergic function of neurons of the SN pars compacta and then death of these neurons. The two phases can be separated by exposure to toxins that induce a transient loss of the DAergic phenotype including amphetamine, MPTP, and α-SYN [17, 61,62,63,64,65]. The protection and functional recovery of these neurons is essential to endorse an early neuroprotective therapy, but such a strategy needs a fine knowledge of the molecules that participate in maintaining the DAergic phenotype during adulthood and the mechanisms that subvert their activity [66, 67].
Here, we focused our study on NURR1 because α-SYN inhibits expression of DAergic genes probably by altering NURR1 expression or activity [68,69,70]. Those studies and also ours assessed the role of aggregated α-SYN, but we cannot discard at this time that monomeric α-SYN might also participate in GSK-3 activation. In fact, ectopic overexpression of α-SYN elicited a reduction in NURR1 expression as indicated by the luciferase reporter (Fig. 2D) that might be related to monomeric α-SYN, although we cannot determine if a fraction of exogenously expressed α-SYN was aggregated. Furthermore, in the Tet-off inducible model in SH-SY5Y cells used here [46], α-SYN produces Triton-soluble and insoluble oligomers, which could be responsible for regulation of the GSK-3/NURR1 axis. Further studies are required under very carefully controlled conditions to finely determine the contribution of monomeric, oligomeric soluble and insoluble, and fibrillary forms of α-SYN to the regulation of GSK-3 and NURR1 protein levels.
Thus, two recent postmortem studies show that the levels RET receptor are reduced by about 80% in nigral neurons containing α-SYN inclusions, leading to impaired RET signaling [71] and probably explaining the limited benefit of GDNF/NRTN-therapeutics in humans [71] and rodent models [72]. This reduction is most likely linked to impaired NURR1 activity because conditional expression of mutant α-SYN in the midbrain DA neurons causes NURR1 degradation and progressive neurodegeneration [22].
α-SYN might inhibit NURR1 gene expression, promote NURR1 protein degradation, or both. The NURR1 coding gene contains an NF-kB site in its promoter, and it has been reported that α-SYN down-regulates NURR1 through inhibition of NF-κB [73]. However, in our cellular remodels, we have detected a very minor reduction in NURR1 mRNA levels. Our results are in line with other studies that report exacerbated NURR1 degradation by the UPS, and NURR1 stabilization via proteasomal inhibition ameliorates degeneration of mDA neurons induced by α-synuclein [22, 74]. Considering that α-SYN is expressed in many tissues and cells, it is likely that different forms of NURR1 regulation operate under specific conditions.
Previous reports have found that GSK-3 phosphorylates α-SYN in a similar manner to TAU [33, 36]. More relevant to our study, α-SYN appears to activate GSK-3β by ill-defined mechanisms that might be related to the formation a complex containing α-SYN/GSK-3β/TAU [27] or by downregulation of signaling pathways such as retinoic acid [24]. Thus, GSK-3β is robustly activated in MPTP models of Parkinsonism, in transgenic mice overexpressing α-SYN, and in the striatum of PD patients [28]. However, we show for the first time that activation of GSK-3β by aggregated α-SYN leads to phosphorylation and subsequent UPS degradation of NURR1, leading to loss of DAergic markers. Mechanistically, we identify a region between 123 and 134 amino acids, harboring proline-directed residues, as the main target of this kinase. Thus, we identify Core 2 as a phosphorylation-dependent degradation domain, phosphodegron, which leads to its UPS degradation.
Human NURR1 contains 61 serines, 27 threonines, and 20 tyrosines distributed along 598 amino acids, and most likely, it is submitted to posttranslational modifications by several protein kinases. NURR1 phosphorylation has been reported to occur by AKT at Ser347 leading to increased protein stability [59], and by RSK/MSK at the same residue [75]. Nine of the putative phosphorylation residues are followed by a proline, and might be phosphorylated by proline-directed kinases such as MAP kinases. In fact, ERK2 phosphorylates NURR1 on multiple sites in in vitro kinase assays, including Ser126 and Thr132, which are located at Core 2. Luciferase reporter assays with a reporter plasmid containing 1 kb of the TH promoter further suggested that these phosphosites are required for ERK2 regulation in SH-SY5Y cells [76]. Contrary to ERK2, GSK-3 is a kinase that remains inactive in the presence of serum and requires growth factor deprivation such as GDNF-deficiency for its activation [77]. Therefore, it is conceivable that Core 2 functions as a molecular switch in coordination with other signals that ultimately will lead to NURR1 activation or to proteasomal degradation. On the other hand, many GSK-3 substrates need to be previously phosphorylated by another kinase in order to be recognized by GSK-3. Therefore, it is also possible that an initial phosphorylation, originated by ERK, might lead to transient NURR1 activation and, at the same time, the ERK-phosphorylated NURR1 would be primed for degradation at a later stage when cell signalling is reduced and GSK-3 becomes active. Such a mechanism would provide a control for over-activation of NURR1. However, under pathological conditions, where neurotrophin signaling is limiting, an imbalance in GSK-3 activity would favor NURR1 degradation over activation.
Although the degradation of NURR1 by the UPS has been reported previously, our study is the first to connect mechanistically this fact with pathology. One study identified Ser347 as a site for phosphorylation by AKT that marks this transcription factor for proteasomal degradation. However, the relevance of AKT leading to the degradation of NURR1 is not clear considering that AKT is a survival kinase that should protect NURR1. In fact, AKT phosphorylates GSK-3-α and β at Ser21 and Ser9, respectively, in their pseudosubstrate domain, leading to its inhibition. It is therefore likely that signals that activate AKT will stabilize NURR1 through the inhibition of GSK-3. Another study identified the first 31 N-terminal residues of NURR1 as a target of proteasomal degradation in several cell types [58]. This study was conducted under standard growth conditions and therefore did not provide a direct link with pathology. It is very likely that NURR1, as several other proteins, might have several motifs for UPS targeting. In fact, the Core 2 mutant still incorporates ubiquitin to some extent.
The finding that α-SYN aggregates reduce the dopaminergic phenotype by GSK-3-mediated degradation of NURR1 suggests that GSK-3 inhibitors might be a therapeutic option to preserve the nigrostriatal track in synucleinopathies such as Parkinson’s disease.
Data Availability
Data will be made available on reasonable request.
Code Availability
Not applicable.
Change history
25 February 2022
The original version of this paper was updated to add the missing compact agreement Open Access funding note.
References
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Acknowledgements
We would like to thank Maria Argyrofthalmidou and Athanasios D. Spathis for their help with α-SYN luciferase and the myc-tagged NURR1 experiments.
Funding
Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) (grant PID2019-110061RB-I00 for A.C and PID2019-105600RB-I00 for I.L.B.) and The Autonomous Community of Madrid (grant B2017/BMD-3827 for A.C. and B2017/BMD-3813 for I.L.B.).
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Highlights
• Aggregated α-synuclein reduces NURR1 protein levels and its target genes, compromising the dopaminergic phenotype.
• Aggregated α-synuclein increases the activity of GSK-3β.
• GSK-3β phosphorylates NURR1 in the “Core 2.”
• Phosphorylated NURR1 is targeted for ubiquitin-proteasome degradation.
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García-Yagüe, Á.J., Lastres-Becker, I., Stefanis, L. et al. α-Synuclein Induces the GSK-3-Mediated Phosphorylation and Degradation of NURR1 and Loss of Dopaminergic Hallmarks. Mol Neurobiol 58, 6697–6711 (2021). https://doi.org/10.1007/s12035-021-02558-9
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DOI: https://doi.org/10.1007/s12035-021-02558-9