Abstract
In the last decades the prevalence of obesity has increased dramatically, and the worldwide epidemic of obesity and related metabolic diseases has contributed to an increased interest for the adipose tissue (AT), the primary site for storage of lipids, as a metabolically dynamic and endocrine organ. Subcutaneous AT is the depot with the largest capacity to store excess energy and when its limit for storage is reached hypertrophic obesity, local inflammation, insulin resistance and ultimately type 2 diabetes (T2D) will develop. Hypertrophic AT is also associated with a dysfunctional adipogenesis, depending on the inability to recruit and differentiate new mature adipose cells. Lately, cellular senescence (CS), an aging mechanism defined as an irreversible growth arrest that occurs in response to various cellular stressors, such as telomere shortening, DNA damage and oxidative stress, has gained a lot of attention as a regulator of metabolic tissues and aging-associated conditions. The abundance of senescent cells increases not only with aging but also in hypertrophic obesity independent of age. Senescent AT is characterized by dysfunctional cells, increased inflammation, decreased insulin sensitivity and lipid storage. AT resident cells, such as progenitor cells (APC), non-proliferating mature cells and microvascular endothelial cells are affected with an increased senescence burden. Dysfunctional APC have both an impaired adipogenic and proliferative capacity. Interestingly, human mature adipose cells from obese hyperinsulinemic individuals have been shown to re-enter the cell cycle and senesce, which indicates an increased endoreplication. CS was also found to be more pronounced in mature cells from T2D individuals, compared to matched non-diabetic individuals, with decreased insulin sensitivity and adipogenic capacity.
Graphical abstract
Factors associated with cellular senescence in human adipose tissue
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Introduction
Due to the worldwide epidemic of obesity and type 2 diabetes (T2D), the adipose tissue (AT) biology and physiology has received increased attention. Nowadays, AT has gone from being only considered as a fat storing tissue to a highly active metabolic and endocrine organ with impact on the whole-body (Ottaviani et al. 2011). However, AT is still the primary site for accumulation of excess energy to be used whenever the demands arise. Not only lipogenesis is of importance also lipolysis has to be tightly regulated and both obesity and T2D could have detrimental effects on adipose tissue metabolism (Singla et al. 2010).
Cellular senescence (CS) is induced as a stress response and defined as an irreversible cell cycle arrest with antitumorigenic effects. Senescent cells cease to proliferate but are still metabolic active and they adopt specific characteristics, with increased cellular growth and secretion of various factor called the senescence-associated secretory phenotype (SASP). Senescent cells have also impact on several other processes, such as wound healing, fibrosis and aging. Lately, CS has also gained a lot of attention due to its involvement in the development and progression of metabolic disorders, such as obesity and T2D (Burton et al.).
The importance of a healthy adipose tissue
Adipose tissue depots and resident cells
AT is distributed throughout the whole body and divided into the main adipose depots, subcutaneous AT (SAT), located under the skin, and visceral AT (VAT), within the abdominal wall and surrounding the internal organs (Shen et al. 2003). SAT is the largest adipose depot and stores about 80% of total body fat and VAT constitutes around 10–20% (Janochova et al. 2019) and for both SAT and VAT the depot of interest is found in the abdomen. Increased VAT is well known to associate with metabolic complication, such as insulin resistance (IR), increased risk for T2D and cardiovascular disease (CVD).
AT is composed of a wide variety of cell types, mature adipose cells of various size, adipose tissue derived progenitor cells (APC), microvascular endothelia cells (MVEC), and different immune cells. Even though mature adipose cells only account for 20–40% of AT resident cells they occupy more or less the whole tissue (Eto et al. 2009). The size of mature adipose cells has been widely used as an important factor for relating cellular functions and pathophysiological conditions.
The size of mature adipose cells as a predictor of metabolic disease
Healthy AT expands through a combination of hypertrophy (enlargement of mature adipose cells) and hyperplasia (recruitment and differentiation of APC). Mature adipose cell growth per se is a necessary mechanism to be able to accommodate excess nutrients and store as triglycerides. SAT is the depot that has the largest capacity to store lipids and, if needed, recruiting new APC will protect against metabolic disease by preventing hypertrophic expansion (Gustafson et al. 2015b; Laforest et al. 2015). However, when SAT has reached its limitation for expansion hypertrophic obesity, local inflammation and IR will emerge (Neeland et al. 2012; Weisberg et al 2003) (Fig. 1).The size of mature adipose cells is influenced by obesity, gender and anatomical localization, and varies considerably in cell diameter, from 20 μm to several hundred μm in humans (Fang et al. 2015; Kloting et al. 2010). Based on a recent publication it was shown that the mean diameter of mature adipose cells in SAT from lean individuals was 75–80 μm and that it increased with body mass index (BMI) and reached a plateau at 120 μm in extremely obese subjects (Ye et al. 2022).
Obesity is known to be a major risk factor for T2D (Kahn and Flier 2000) and visceral obesity has been associated with a higher risk of metabolic disease compared to subcutaneous obesity with little or no risk or even being protective (Tchkonia et al. 2010). Adipose cell hypertrophy has been shown to be more highly associated with IR, lipid metabolism, T2D diabetes and cardiovascular disorders than the effect of obesity alone (Ye et al. 2022) (Fig. 1).
A positive correlation between SAT adipose cell size and IR, independent of BMI in non-diabetic individuals has been found. However, this was not seen in T2D individuals, which suggests that other factors, such as free fatty acids and glucose, adds to the effect of adipose cell size (Lundgren et al. 2007). Furthermore, in a cohort of individual, highly variable in BMI, adipose cell size was positively correlated with IR, measured as HOMA index, and insulin levels (Arner et al. 2010). It has also been shown that IR individuals, compared to BMI-matched insulin-sensitive individuals, are characterized by enlargement of large mature adipose cells, a larger proportion of small adipose cells together with decreased expression of the differentiation markers, peroxisome proliferator-activated receptor g 2 (PPARG2), solute carrier family 2 member 4 (also known as GLUT4), and adiponectin, which suggests an impaired adipogenesis (McLaughlin et al. 2007, 2014). This was also shown in T2D individuals compared to obese individuals with the same BMI (Pasarica et al. 2009). Further, in young and non-obese first-degree relatives of type 2 diabetics (FDR), increased IR correlated with inappropriately expanded adipose cells for their BMI, which was not seen in matched individuals with family predisposition for obesity (Yang et al. 2012). This was subsequently documented in a large group of FDR independent of obesity (Arner et al. 2011). It has also been demonstrated in populations with different ethnicity that enlarged SAT mature adipose cells are associated with IR and could predict the incidence of T2D independent of body fat and BMI (Weyer et al. 2000; Lonn et al. 2010). In a recent study, adipose cell hypertrophy was found to be associated with telomere shortening, as a marker of biological or premature aging, low levels of adiponectin and increased oxidative stress in obese and T2D individuals (Monickaraj et al. 2012). Taken together, adipose cell hypertrophy, independent of obesity and BMI, is associated with IR and predicts T2D, not only in non-obese individuals of different ethnicity but also in non-obese individuals with a genetic predisposition for T2D (Fig. 1). SAT adipose cell hypertrophy also suggests an impaired adipogenesis due to an inability to recruit and differentiate new mature adipose cells (Gustafson et al. 2013). However, hypertrophic obesity is also associated with an increased lipolysis, which is further aggravated by an impaired anti-lipolytic effect of insulin (Smith and Kahn 2016).
Obesity, insulin resistance and chronic low-grade inflammation
Numerous studies have identified a close association between obesity, IR and chronic low-grade inflammation (Hammarstedt et al. 2018) (Fig. 1) and both human and animal studies have shown that AT is the primary site for the initiation and aggravation of obesity-related chronic low-grade inflammation (Weisberg et al. 2003; Xu et al. 2003). However, it is not an increased fat mass per se but rather a dysfunctional SAT with hypertrophic mature adipose cells that drives the induction of chronic low-grade inflammation and IR in metabolically unhealthy subjects. This remodeling of AT triggers the activation of the mitogen-activated protein kinase 8 (also known as JNK1) and nuclear factor kappa B (NF-kB) signaling pathways, which have negative impacts on insulin signaling as well as increased expression of several proinflammatory genes. This also increases the infiltration and activation of inflammatory macrophages, known to produce and secrete chemokines and proinflammatory cytokines, such as interleukin 6 (IL6) and tumor necrosis factor-α (TNFα), where both have been shown to directly inhibit insulin signaling (Zatterale et al. 2019).
A dysfunctional AT not only adopts a proinflammatory phenotype that is harmful for microenvironment, the increased storage of free fatty acids leads also to increased lipolysis, enhanced endoplasmic reticulum and oxidative stress, and mitochondrial and lysosomal dysfunction (Ahmed et al. 2021). Lately, these complications due to a hypertrophic AT has also been suggested to be associated with CS (Narasimhan et al. 2021), which will be discussed later in this review (Fig. 1).
Hypertrophic obesity and adipogenesis
For a more detailed review of adipogenesis, which is out of scope in this article, we refer to our recent publication by Hammarstedt et al. (2018). Adipogenesis is a highly complex process, which starts with the recruitment and commitment of APC into preadipocytes followed by terminal differentiation into mature adipose cells. Before entering the final differentiation step the recruited APC have to undergo mitotic clonal expansion, which is regulated by the bone morphogenetic protein 4 (BMP4) (Bowers et al. 2006). BMP4 dissociates a differentiation inhibitory complex, including the transcriptional coactivator zinc finger protein 423 (ZNF423). ZNF423 will enter the nucleus and activate the transcription of PPARG (Hammarstedt et al. 2013), the master regulator of adipogenic differentiation together with the transcription factor CCAAT enhancer binding protein alpha (Wu et al. 1999). This allows the cells to mature with the ability to process and store lipids, secrete adipokines, such as adiponectin (known to enhance insulin sensitivity in peripheral tissues (Turer and Scherer 2012)) and BMP4, respond to insulin and to maintain the phenotype of mature adipose cells. However, the effect of BMP4 is strongly regulated by BMP inhibitors, such as Gremlin 1 (Topol et al. 2000).
As mentioned above a decreased adipogenic potential has been seen in hypertrophic obesity. However, it is not the number of human subcutaneous APC per se that is reduced, instead it is the number or cells that has the capacity to undergo differentiation that is decreased (Gustafson et al. 2019). In hypertrophic obesity the phenotype is changed, and the adipose cells become IR, which is associated with a proinflammatory profile with increased levels of cytokines and chemokines as well as increased infiltration of macrophages. These macrophages further promote the inflammatory phenotype including secretion of TNFα (Gustafson et al. 2009). Furthermore, TNFα has been shown to completely inhibit the adipogenic potential of APC and to induce a macrophage-like phenotype in these cells (Isakson et al. 2009).
Cellular senescence in adipose tissue
Overview
Replicative senescence was first described by Hayflick and Moorhead in cultured human fibroblasts. Upon repeated serial passing these cells showed a limited replicative capacity and become irreversibly arrested, which was considered as a hallmark of aging (Hayflick and Moorhead 1961). Nowadays, senescence is defined as a process where cells age and become permanently cell cycle arrested but still are alive, metabolically active and resistant to apoptosis (Kumari and Jat 2021). CS is a highly dynamic process driven by both tissue- and context-specific effects as well as epigenetic and genetic changes (van Deursen 2014). There are two main types of senescence, replicative senescence, as previously mentioned, and stress-induced premature senescence (SIPS). SIPS can be induced by variety of stressors, such as oxidative stress, metabolic dysfunction, epigenetic alteration, radiation, endoplasmic reticulum (ER) stress, genotoxic drugs as well as mechanical and shear stress (Gorgoulis et al. 2019). However, depending on the inducer of CS, different pathways and factors will be affected leading to DNA damage, telomere shortening, inflammation, mitochondrial dysfunction, hypoxia, oncogene activation and increased secretion of cytokines, chemokines, proteases and growth factors, known as the senescence-associated secretory phenotype (SASP) and decreased cell differentiation (Stout et al. 2017; Gustafson et al. 2019; Gorgoulis et al. 2019). Specific SASP factors vary depending on cell and tissue origin but also on the inducers of senescence. However, IL6 and IL8 are mostly found to be present (Coppe et al. 2010) and TNFα is also secreted by a large variety of cells (Kandhaya-Pillai et al. 2017). SASPs act through both autocrine, by re-enforcing CS, and paracrine factors, by inducing senescence in neighboring non-senescent cells which further contributes to the age-related disorders (Acosta et al. 2013). Taken together, due to secreted SASP factors senescent cell can be a source for the low-grade chronic inflammation seen in the microenvironment of a dysfunctional AT. However, CS has not only detrimental effects it has also certain beneficial effects on embryogenesis, wound healing and tumor suppression (Gorgoulis et al. 2019).
Senescent cells undergo changes in morphology and senescence-associated proteins. In culture, these cells appear to be larger, flattened, have more granules in the cytoplasm, multinucleated, more vacuoles and the nucleus shows signs of DNA damage with increased phosphorylation of the H2A histone family X (γH2AX) (Beck et al. 2020). Furthermore, senescent cells have disrupted nuclear membrane due to decreased expression of lamin B1 (LMNB1), a key trigger of global chromatin reconfiguration, as well as chromatin reorganization (Liu et al. 2020). Also, the lysosomal compartment is largely increased in senescent cells, which is reflected by an increased activity of the characteristic senescence-associated β-galactosidase (SA-β-Gal) (Rovira et al. 2022).
To establish a senescent cell-cycle arrest, due to an unresolved DNA damage, the activation of p53 (encoded by tumor protein p53 (TP53)) and/or retinoblastoma (RB) tumor suppressor pathways play a central role, which leads to the accumulation of the cyclin dependent kinase (CDK) 2 inhibitor p21 (encoded by cyclin dependent kinase inhibitor (CDKN) 1A) and CDK4/6 inhibitor p16 (encoded by CDKN2A). As a consequence of this, RB will be activated and result in a persistent cell-cycle arrest (Gorgoulis et al. 2019). One important feature of senescent cells is to avoid apoptosis and resist self-clearance, which they do by activating a network of antiapoptotic senescent cell pathways (SCAPs), such as B-cell lymphoma (BCL) 2/BCL-extra large (XL), phosphatidylinositol 4,5-bisphosphate 3-kinase (PI3K)/ serine/threonine kinase 1 (AKT), or p53/p21/serpine (Kirkland and Tchkonia 2017). Since both senescence and apoptosis trigger the p53 pathway it has been suggested that the level and activity of p53, are of most importance for senescent cells to resist apoptosis and to enter a permanent cell cycle arrest (Kirschner et al. 2015). Even though senescent cells share common characteristics, these do not always occur at the same time and intensity, making the senescence phenotype extremely dynamic and complex. Therefore, it is of major importance to use multiple markers to determine if the cells have adopted a senescent phenotype (Liu et al. 2020).
Both CS and SASP are potential therapeutic targets and senotherapeutics, a new class of drugs and natural products targeting CS, is under development. Senotherapeutics consist of two members, senomorphics and senolytics. Senolytics act directly on the senescent cells by selectively killing them through apoptosis. On the contrary, senomorphic compounds act, without killing senescent cells, on pathologic SASP signaling pathways by preventing the production of, neutralizing or antagonizing SASP factors (Lagoumtzi and Chondrogianni 2021).
Cellular senescence and adipose tissue
AT is an endocrine organ known to be important for maintenance of lipid and energy homeostasis as well as systemic glucose levels. However, these functions decrease with aging and obesity. It is well known that aging is a major risk factor for many chronic states/diseases, including IR, T2D, nonalcoholic fatty liver disease (NAFLD), and CVD (Tchkonia and Kirkland 2018). Many of these chronic diseases show a state of premature or accelerated aging. Biological aging could be defined as a state of replicative senescence and, lately, much attention has been paid to CS as a regulator of metabolic tissues and as a contributor to aging-associated conditions. Recently, the Tabula Muris study showed an age-associated increase in tissue/CS in mice contributing to dysfunctional cells, increased inflammation and accumulation of markers of genetic instability (Tabula Muris 2020). Furthermore, AT is one of the most vulnerable tissues when it comes to aging (Ou et al. 2022) and also a tissue where CS according to an animal study is initiated most early (Tabula Muris 2020). The two main adipose depots, SAT and VAT, are differentially associated with the clinical outcomes of obesity and aging as discussed above. It has also been shown that the telomere length, as a marker of risk of CS, is shorter in SAT than in VAT suggesting that SAT is more prone to age-related injuries (Lakowa et al. 2015).
Cellular senescence and its consequences for adipogenesis
There is a need for an annual renewal of 10% of mature adipose cells in adulthood (Spalding et al. 2008) and to maintain the functionality of the AT, new cells has to be recruited into the adipogenic program. The first step is to recruit APC, which are committed into preadipocytes followed by the second step of differentiation to become mature adipose cells. CS has a high impact on AT with a dysfunctional adipogenesis as result.
In earlier studies we have shown that the adipogenic potential of APC from human SAT has a high inter-individual variability and that markers for reduced adipogenesis, such as PPARγ and reduced lipid accumulation, reduced insulin sensitivity and increased inflammation correlate with hypertrophic obesity and genetic predisposition for T2D in FDR (Gustafson et al. 2013, 2015a, 2015b; Arner et al. 2011). However, both the proliferative as well as the differentiation capacity of senescent APC are reduced (Xu et al. 2015b; Mitterberger et al. 2014; Gustafson et al. 2019) (Fig. 2). More recently, we have shown that the number of human subcutaneous APC in hypertrophic obesity and T2D are not reduced, instead these cells have a dysfunctional adipogenesis due to increased CS with high levels of p53 and p16 (Gustafson et al. 2019). Since p53 has a central role in the induction of CS and has to be downregulated to allow the adipogenic program to progress, this could be one of the reasons for the impaired adipogenesis. It was also shown that human senescent APC cells secrete activin A, a SASP factor known to affect adipogenesis negatively, which rendered non-senescent APC senescent and suppressed their adipogenic capacity (Xu et al. 2015b). In the same study senescent cells, in 18-month-old mice, were targeted by janus kinase (JAK) inhibitors, which blunted activin A and increased adipogenesis (Xu et al. 2015b). Not only senescent cells per se but also the paracrine effect of secreted SASP factors have a massive impact on the adipogenic potential of neighboring non-senescent APC (Fig. 2).
Cellular senescence in adipose tissue derived progenitor cells (APC)
Not only aging, also the onset of obesity is linked to an increased burden of senescent cells within the adipose tissue, and it has been reported that APC can senesce due to replicative senescence in aging or by increased oxidative stress in obesity (Xu et al. 2015a; Mitterberger et al. 2014).
In an early study, a link between increased p53 expression in AT and obesity, senescence, and age-related metabolic disorders was found (Minamino et al. 2009). Consistent with this, APC from obese individuals exhibited a limited replicative potential as well as increased expression of senescence markers, TP53, CDKN1A, CDKN2A, IL6 and C–C motif chemokine ligand 2 (CCL2) compared to lean individuals (Conley et al. 2020). Also, in subcutaneous APC from young obese mice increased levels of p21 and p16 were detected (Alessio et al. 2020). Furthermore, it was shown in human SAT that the senescence markers, TP53, galactosidase beta 1 (GLB1) and serpin family E member 1 (SERPINE1), were increased in hypertrophic obesity and further increased in similarly obese T2D individuals. For APC to enter the adipogenic program p53 must be downregulated, which turned out to be impaired in human senescent APC, as discussed above (Gustafson et al. 2019). This was also valid for APC from FDR, suggesting that increased APC senescence is associated with reduced adipogenesis before the onset of T2D (Gustafson et al. 2019).
Comparing a panel of senescence markers in APC from FDR and control individuals, increased number of SA-β-gal and p21 positive cells together with increased number of cells arrested in the G1 phase of the cell cycle was found as well as increased levels of secreted SASPs (Spinelli et al. 2022). It is well known that senescent APC affect neighboring non-senescent APC via secreted SASP factors, such as activin A, IL6 and TNFα contributing to impaired adipogenesis as well as reduced insulin sensitivity (Zaragosi et al. 2010; Xu et al. 2015a, 2015b) and also likely involved in the development of T2D. Furthermore, altered DNA methylation is a powerful epigenetic modification that has been implicated in the development of CS (Crouch et al. 2022). In a study characterizing the methylome in APC from FDR and matched control individuals it was found that APC from FDR had several hypomethylated genes related to senescence with zinc finger matrin-type 3 (ZMAT3) as one of the top-ranked genes (Parrillo et al. 2020). ZMAT3 hypomethylation in APC from FDR leads to increased levels of ZMAT3 and p53 as well as induced expression of CDKN1A and consequently premature senescence (Spinelli et al. 2022). It was also shown that ZMAT3 interacts with the p53/p21 pathway in APC to induce senescence. Furthermore, in vitro overexpression of ZMAT3 in human APC contributed to induced senescence and reduced adipogenesis (Spinelli et al. 2022).
Cellular senescence in post-mitotic mature adipose cells
Even post-mitotic cells can assume a senescent phenotype, which has been shown in different cells, such as neurons, skeletal muscle fibers, cardiomyocytes and osteocytes, from aging mice (Tsiloulis and Watt 2015). In mature adipose cells from several mouse models, associated with age and obesity, a senescent phenotype has also been documented (Chen et al. 2015; Lee et al. 2022; Liu et al. 2018; Minamino et al. 2009; Vergoni et al. 2016), which recently has been confirmed in human mature adipose cells.
First and unexpectedly, Li et al. found that human subcutaneous mature adipose cells could activate a cell cycle program and that the cell cycle progression was associated with obesity and hyperinsulinemia (Li et al. 2021). Adipose cells from obese hyperinsulinemic individuals expressed G-specific cell cycle markers but not markers for the progression through mitosis, which is indicative of an endoreplicative or post-mitotic cell cycle (Li et al. 2021). This suggests that the cells get arrested in G2-phase there they exit the cell cycle and senesce. In a recent paper, we showed that human subcutaneous mature adipose cells assume a senescent phenotype in obese individuals, and this was even more pronounced in T2D individuals independent of BMI and age (Gustafson et al. 2022) (Fig. 2).γH2AX staining, as a measure of unresolved DNA damage response, the senescence marker CDKN1A as well as the expression of SASP factors (IL6, CCL2, SERPINE1, and IL1B) were increased in mature adipose cells from obese hyperinsulinemic individuals (Li et al. 2021). The senescence markers, TP53, GLB1, and SERPINE1 were induced in mature adipose cells in obesity and even further increased in T2D and the level of senescence correlated to both degree of IR and adipose cell size. However, the insulin levels were not higher in T2D individuals compared to be equally obese non-diabetic individuals, which suggests that chronic hyperinsulinemia in not the only driver of CS in mature adipose cells. Furthermore, ZMAT3 and TNFA were increased in T2D individuals compared to obese individuals of the same age (Gustafson et al. 2022). β-GAL, p16, plasminogen activator inhibitor 1 (PAI1, encoded by SERPINE1), p53 and the phosphorylation of mitogen-activated protein kinase 8 (also known as JNK1) were increased in mature adipose cells, which corresponded to decreased levels of adipogenic markers, PPARγ and GLUT4, and insulin sensitivity measured as serine 473 phosphorylation of AKT in T2D compared to lean individuals (Gustafson et al. 2022) (Fig. 2).
Moreover, high levels of cyclin D1 (encoded by CCND1), an important regulator of cell cycle progression, was detected in obese individuals with hyperinsulinemia (Li et al. 2021). This is in line with our findings of increased CCND1 in mature adipose cells of obese individuals, which was further increased in T2D individuals (Gustafson et al. 2022). CCND1 expression correlated also with cell size and the senescence markers TP53, GLB1, SERPINE1 and ZMAT3. There was a gradual increase in cyclin D1, p16 and β-GAL in mature adipose cells comparing lean, obese and T2D individuals (Gustafson et al. 2022).
The chemotherapeutic agent Doxorubicin, like several other similar anti-cancer drugs, induces senescence in human APC as well as in differentiated cells with increased γH2AX, p21, ZMAT3 and cyclin D1 while the adipogenic markers PPARγ, adiponectin and fatty acid binding protein 4 (FABP4) are decreased as markers of de-differentiation of the cells (Gustafson et al. 2022).
Cellular senescence in adipose tissue derived microvascular endothelial cells
Not only the storage of triglycerides in AT, which is a highly vascularized tissue, is crucial but also the transport of fatty acids through the microvascular endothelium is highly important for normal adipose tissue expansion. Endothelial cells (EC) can remain quiescent for years but, when needed, they become activated and initiate new vessel formation (angiogenesis), which is essential for the function of mature adipose cells as well as adipogenesis (Herold and Kalucka 2020). In obesity, AT is rapidly expanding and if the vascular network is not increased in parallel a dysfunctional AT will appear (Hammarstedt et al. 2018). Senescent EC have also been identified as important contributor to various metabolic and cardiovascular diseases (Bloom et al. 2023).
Interestingly, it was found that VAT-derived MVEC from obese individuals had a more pronounced senescent phenotype than SAT-derived MVEC together with a decreased expression of PPARγ (Villaret et al. 2010). It was also shown that PPARγ activity in human SAT-derived MVEC was regulated by senescence and that the senescent marker CDKN2A increased with aging. Furthermore, PPARγ turned out to be key regulator of fatty acid uptake in MVEC and CDKN2A showed a strong inverse correlation with the expression of the PPARγ target genes, FABP4, CD36 and solute carrier family 27 member 1 (SLC27A1) (Briot et al. 2018). Recently, we have shown that SAT-derived MVEC are highly specialized EC both regulating lipid transport and secreting lipids that activate PPARγ (Gogg et al. 2019). Most important is the crosstalk between MVEC and adipose cells in vivo to regulate the lipid transport and to activate PPARγ in APC to drive the adipogenic program. Senescence in MVEC seems not only to have impact on lipid handling and transport, but it also influences the ability of APC to differentiate with a dysfunctional AT and ectopic fat accumulation as consequences (Fig. 2).
Conclusions and future possibilities
Senescent cells have left the cell cycle and become proinflammatory through secretion of SASP factors which include cytokines, chemokines, miRNA, DNA and other factors which can target ambient cells and induce senescence in these as well. Like many other cells, adipose tissue cells; both progenitor cells, mature adipose cells and microvascular endothelial cells, undergo senescence with aging but it is also increased with other common conditions like obesity and T2D. We only know some factors which can contribute to this including genetic factors and development of hyperinsulinemia. Adipose tissue cell senescence is associated with reduced adipogenesis, impaired adipose cell function, reduced PPARγ and reduced insulin sensitivity. Treating cell senescence in animal models improve many aging-associated conditions including insulin resistance, hyperglycemia and cardiovascular conditions. The current development of senomorphic agents may allow treatment of common aging-associated conditions in man as well but firm proof of this is currently not yet available.
Abbreviations
- AKT:
-
Serine/threonine kinase 1
- APC:
-
Adipose tissue derived progenitor cells
- AT:
-
Adipose tissue
- BCL:
-
B-cell lymphoma
- BMI:
-
Body mass index
- BMP4:
-
Bone morphogenetic protein 4
- CCL2 :
-
C–C motif chemokine ligand 2
- CDK:
-
Cyclin dependent kinase
- CS:
-
Cellular senescence
- CVD:
-
Cardiovascular disease
- EC:
-
Endothelial cells
- FABP4:
-
Fatty acid binding protein 4
- FDR:
-
First-degree relatives of type 2 diabetic
- gH2AX:
-
Phosphorylated H2A histone family X
- GLB1 :
-
Galactosidase beta 1
- IL:
-
Interleukin
- IR:
-
Insulin resistance
- MVEC:
-
Microvascular EC
- PPARγ:
-
Peroxisome proliferator-activated receptor γ
- RB:
-
Retinoblastoma
- SA-β-Gal:
-
Senescence-associated β-galactosidase
- SASP:
-
Senescence-associated secretory phenotype
- SAT:
-
Subcutaneous adipose tissue
- SERPINE1 :
-
Serpin family E member 1
- T2D:
-
Type 2 diabetes
- TNFa:
-
Tumor necrosis factor-α
- TP53 :
-
Tumor protein p53
- VAT:
-
Visceral adipose tissue
- ZNF423:
-
Zinc finger protein 423
- ZMAT3:
-
Zinc finger matrin-type 3
References
Acosta JC, Banito A, Wuestefeld T, Georgilis A, Janich P, Morton JP, Athineos D, Kang TW, Lasitschka F, Andrulis M, Pascual G, Morris KJ, Khan S, Jin H, Dharmalingam G, Snijders AP, Carroll T, Capper D, Pritchard C, Inman GJ, Longerich T, Sansom OJ, Benitah SA, Zender L, Gil J (2013) A complex secretory program orchestrated by the inflammasome controls paracrine senescence. Nat Cell Biol 15:978–990
Ahmed B, Sultana R, Greene MW (2021) Adipose tissue and insulin resistance in obese. Biomed Pharmacother 137:111315
Alessio N, Acar MB, Demirsoy IH, Squillaro T, Siniscalco D, Bernardo GD, Peluso G, Ozcan S, Galderisi U (2020) Obesity is associated with senescence of mesenchymal stromal cells derived from bone marrow, subcutaneous and visceral fat of young mice. Aging (albany NY) 12:12609–12621
Arner E, Westermark PO, Spalding KL, Britton T, Ryden M, Frisen J, Bernard S, Arner P (2010) Adipocyte turnover: relevance to human adipose tissue morphology. Diabetes 59:105–109
Arner P, Arner E, Hammarstedt A, Smith U (2011) Genetic predisposition for type 2 diabetes, but not for overweight/obesity, is associated with a restricted adipogenesis. PLoS ONE 6:e18284
Beck J, Horikawa I, Harris C (2020) Cellular senescence: mechanisms, morphology, and mouse models. Vet Pathol 57:747–757
Bloom SI, Islam MT, Lesniewski LA, Donato AJ (2023) Mechanisms and consequences of endothelial cell senescence. Nat Rev Cardiol 20:38–51
Bowers RR, Kim JW, Otto TC, Lane MD (2006) Stable stem cell commitment to the adipocyte lineage by inhibition of DNA methylation: role of the BMP-4 gene. Proc Natl Acad Sci 103:13022–13027
Briot A, Decaunes P, Volat F, Belles C, Coupaye M, Ledoux S, Bouloumie A (2018) Senescence alters PPARgamma (peroxisome proliferator–activated receptor gamma)-dependent fatty acid handling in human adipose tissue microvascular endothelial cells and favors inflammation. Arterioscler Thromb Vasc Biol 38:1134–1146
Chen YW, Harris RA, Hatahet Z, Chou KM (2015) Ablation of XP-V gene causes adipose tissue senescence and metabolic abnormalities. Proc Natl Acad Sci 112:E4556–E4564
Conley SM, Hickson LJ, Kellogg TA, McKenzie T, Heimbach JK, Taner T, Tang H, Jordan KL, Saadiq IM, Woollard JR, Isik B, Afarideh M, Tchkonia T, Kirkland JL, Lerman LO (2020) Human obesity induces dysfunction and early senescence in adipose tissue-derived mesenchymal stromal/stem cells. Front Cell Dev Biol 8:197
Coppe JP, Desprez PY, Krtolica A, Campisi J (2010) The senescence-associated secretory phenotype: the dark side of tumor suppression. Annu Rev Pathol 5:99–118
Crouch J, Shvedova M, Thanapaul R, Botchkarev V, Roh D (2022) Epigenetic regulation of cellular senescence. Cells. https://doi.org/10.3390/cells11040672
Eto H, Suga H, Matsumoto D, Inoue K, Aoi N, Kato H, Araki J, Yoshimura K (2009) Characterization of structure and cellular components of aspirated and excised adipose tissue. Plast Reconstr Surg 124:1087–1097
Fang L, Guo F, Zhou L, Stahl R, Grams J (2015) The cell size and distribution of adipocytes from subcutaneous and visceral fat is associated with type 2 diabetes mellitus in humans. Adipocyte 4:273–279
Gogg S, Nerstedt A, Boren J, Smith U (2019) Human adipose tissue microvascular endothelial cells secrete PPARgamma ligands and regulate adipose tissue lipid uptake. JCI Insight. https://doi.org/10.1172/jci.insight.125914
Gorgoulis V, Adams PD, Alimonti A, Bennett DC, Bischof O, Bishop C, Campisi J, Collado M, Evangelou K, Ferbeyre G, Gil J, Hara E, Krizhanovsky V, Jurk D, Maier AB, Narita M, Niedernhofer L, Passos JF, Robbins PD, Schmitt CA, Sedivy J, Vougas K, von Zglinicki T, Zhou D, Serrano M, Demaria M (2019) Cellular senescence: defining a path forward. Cell 179:813–827
Gustafson et al (2009) Inflammation and impaired adipogenesis in hypertrophic obesity in man. Am J Physiol Endocrinol Metab 297(5):E999–E1003
Gustafson B, Hammarstedt A, Hedjazifar S, Smith U (2013) Restricted adipogenesis in hypertrophic obesity: the role of WISP2, WNT, and BMP4. Diabetes 62:2997–3004
Gustafson B, Hammarstedt A, Hedjazifar S, Hoffmann JM, Svensson PA, Grimsby J, Rondinone C, Smith U (2015a) BMP4 and BMP antagonists regulate human white and beige adipogenesis. Diabetes 64:1670–1681
Gustafson B, Hedjazifar S, Gogg S, Hammarstedt A, Smith U (2015b) Insulin resistance and impaired adipogenesis. Trends Endocrinol Metab 26:193–200
Gustafson B, Nerstedt A, Smith U (2019) Reduced subcutaneous adipogenesis in human hypertrophic obesity is linked to senescent precursor cells. Nat Commun 10:2757
Gustafson B, Nerstedt A, Spinelli R, Beguinot F, Smith U (2022) Type 2 diabetes, independent of obesity and age, is characterized by senescent and dysfunctional mature human adipose cells. Diabetes 71:2372–2383
Hammarstedt A, Hedjazifar S, Jenndahl L, Gogg S, Grunberg J, Gustafson B, Klimcakova E, Stich V, Langin D, Laakso M, Smith U (2013) WISP2 regulates preadipocyte commitment and PPARgamma activation by BMP4. Proc Natl Acad Sci 110:2563–2568
Hammarstedt A, Gogg S, Hedjazifar S, Nerstedt A, Smith U (2018) Impaired adipogenesis and dysfunctional adipose tissue in human hypertrophic obesity. Physiol Rev 98:1911–1941
Hayflick L, Moorhead PS (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25:585–621
Herold J, Kalucka J (2020) Angiogenesis in adipose tissue: the interplay between adipose and endothelial cells. Front Physiol 11:624903
Isakson P, Hammarstedt A, Gustafson B, Smith U (2009) Impaired preadipocyte differentiation in human abdominal obesity: role of Wnt, tumor necrosis factor-alpha, and inflammation. Diabetes 58:1550–1557
Janochova K, Haluzik M, Buzga M (2019) Visceral fat and insulin resistance - what we know? Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 163:19–27
Kahn BB, Flier JS (2000) Obesity and insulin resistance. J Clin Invest 106:473–481
Kandhaya-Pillai R, Miro-Mur F, Alijotas-Reig J, Tchkonia T, Kirkland JL, Schwartz S (2017) TNFalpha-senescence initiates a STAT-dependent positive feedback loop, leading to a sustained interferon signature, DNA damage, and cytokine secretion. Aging (albany NY) 9:2411–2435
Kirkland JL, Tchkonia T (2017) Cellular senescence: a translational perspective. EBioMedicine 21:21–28
Kirschner K, Samarajiwa SA, Cairns JM, Menon S, Perez-Mancera PA, Tomimatsu K, Bermejo-Rodriguez C, Ito Y, Chandra T, Narita M, Lyons SK, Lynch AG, Kimura H, Ohbayashi T, Tavare S, Narita M (2015) Phenotype specific analyses reveal distinct regulatory mechanism for chronically activated p53. PLoS Genet 11:e1005053
Kloting N, Fasshauer M, Dietrich A, Kovacs P, Schon MR, Kern M, Stumvoll M, Bluher M (2010) Insulin-sensitive obesity. Am J Physiol Endocrinol Metab 299:E506–E515
Kumari R, Jat P (2021) Mechanisms of cellular senescence: cell cycle arrest and senescence associated secretory phenotype. Front Cell Dev Biol 9:645593
Laforest S, Labrecque J, Michaud A, Cianflone K, Tchernof A (2015) Adipocyte size as a determinant of metabolic disease and adipose tissue dysfunction. Crit Rev Clin Lab Sci 52:301–313
Lagoumtzi SM, Chondrogianni N (2021) Senolytics and senomorphics: natural and synthetic therapeutics in the treatment of aging and chronic diseases. Free Radic Biol Med 171:169–190
Lakowa N, Trieu N, Flehmig G, Lohmann T, Schon MR, Dietrich A, Zeplin PH, Langer S, Stumvoll M, Bluher M, Kloting N (2015) Telomere length differences between subcutaneous and visceral adipose tissue in humans. Biochem Biophys Res Commun 457:426–432
Lee G, Kim YY, Jang H, Han JS, Nahmgoong H, Park YJ, Han SM, Cho C, Lim S, Noh JR, Oh WK, Lee CH, Kim S, Kim JB (2022) SREBP1c-PARP1 axis tunes anti-senescence activity of adipocytes and ameliorates metabolic imbalance in obesity. Cell Metab 34(702–718):e5
Li Q, Hagberg CE, Silva Cascales H, Lang S, Hyvonen MT, Salehzadeh F, Chen P, Alexandersson I, Terezaki E, Harms MJ, Kutschke M, Arifen N, Kramer N, Aouadi M, Knibbe C, Boucher J, Thorell A, Spalding KL (2021) Obesity and hyperinsulinemia drive adipocytes to activate a cell cycle program and senesce. Nat Med 27:1941–1953
Liu Z, Jin L, Yang JK, Wang B, Wu KKL, Hallenborg P, Xu A, Cheng KKY (2018) The dysfunctional MDM2-p53 axis in adipocytes contributes to aging-related metabolic complications by induction of lipodystrophy. Diabetes 67:2397–2409
Liu Z, Wu KKL, Jiang X, Xu A, Cheng KKY (2020) The role of adipose tissue senescence in obesity- and ageing-related metabolic disorders. Clin Sci (lond) 134:315–330
Lonn M, Mehlig K, Bengtsson C, Lissner L (2010) Adipocyte size predicts incidence of type 2 diabetes in women. FASEB J 24:326–331
Lundgren et al (2007) Fat cell enlargement is an independent marker of insulin resistance and ‘hyperleptinaemia’. Diabetologia 50(3):625–633
Minamino T, Orimo M, Shimizu I, Kunieda T, Yokoyama M, Ito T, Nojima A, Nabetani A, Oike Y, Matsubara H, Ishikawa F, Komuro I (2009) A crucial role for adipose tissue p53 in the regulation of insulin resistance. Nat Med 15:1082–1087
Mitterberger MC, Lechner S, Mattesich M, Zwerschke W (2014) Adipogenic differentiation is impaired in replicative senescent human subcutaneous adipose-derived stromal/progenitor cells. J Gerontol A Biol Sci Med Sci 69:13–24
Monickaraj F, Gokulakrishnan K, Prabu P, Sathishkumar C, Anjana RM, Rajkumar JS, Mohan V, Balasubramanyam M (2012) Convergence of adipocyte hypertrophy, telomere shortening and hypoadiponectinemia in obese subjects and in patients with type 2 diabetes. Clin Biochem 45:1432–1438
McLaughlin et al (2007) Enhanced proportion of small adipose cells in insulin-resistant vs insulin-sensitive obese individuals implicates impaired adipogenesis. Diabetologia 50(8):1707–1715
McLaughlin et al (2014) Subcutaneous adipose cell size and distribution: relationship to insulin resistance and body fat. Obesity (Silver Spring) 22(3):673–680
Narasimhan A, Flores RR, Robbins PD, Niedernhofer LJ (2021) Role of cellular senescence in type II diabetes. Endocrinology. https://doi.org/10.1210/endocr/bqab136
Neeland et al (2012) Dysfunctional adiposity and the risk of prediabetes and type 2 diabetes in obese adults. J AMA 308(11):1150–1159
Ottaviani E, Malagoli D, Franceschi C (2011) The evolution of the adipose tissue: a neglected enigma. Gen Comp Endocrinol 174:1–4
Ou MY, Zhang H, Tan PC, Zhou SB, Li QF (2022) Adipose tissue aging: mechanisms and therapeutic implications. Cell Death Dis 13:300
Parrillo L, Spinelli R, Longo M, Desiderio A, Mirra P, Nigro C, Fiory F, Hedjazifar S, Mutarelli M, Carissimo A, Formisano P, Miele C, Smith U, Raciti GA, Beguinot F (2020) Altered PTPRD DNA methylation associates with restricted adipogenesis in healthy first-degree relatives of Type 2 diabetes subjects. Epigenomics 12:873–888
Pasarica M, Xie H, Hymel D, Bray G, Greenway F, Ravussin E, Smith SR (2009) Lower total adipocyte number but no evidence for small adipocyte depletion in patients with type 2 diabetes. Diabetes Care 32:900–902
Rovira M, Sereda R, Pladevall-Morera D, Ramponi V, Marin I, Maus M, Madrigal-Matute J, Diaz A, Garcia F, Munoz J, Cuervo AM, Serrano M (2022) The lysosomal proteome of senescent cells contributes to the senescence secretome. Aging Cell 21:e13707
Shen W, Wang Z, Punyanita M, Lei J, Sinav A, Kral JG, Imielinska C, Ross R, Heymsfield SB (2003) Adipose tissue quantification by imaging methods: a proposed classification. Obes Res 11:5–16
Singla P, Bardoloi A, Parkash AA (2010) Metabolic effects of obesity: a review. World J Diabetes 1:76–88
Smith U, Kahn BB (2016) Adipose tissue regulates insulin sensitivity: role of adipogenesis, de novo lipogenesis and novel lipids. J Intern Med 280:465–475
Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Naslund E, Britton T, Concha H, Hassan M, Ryden M, Frisen J, Arner P (2008) Dynamics of fat cell turnover in humans. Nature 453:783–787
Spinelli R, Florese P, Parrillo L, Zatterale F, Longo M, D’Esposito V, Desiderio A, Nerstedt A, Gustafson B, Formisano P, Miele C, Raciti GA, Napoli R, Smith U, Beguinot F (2022) ZMAT3 hypomethylation contributes to early senescence of preadipocytes from healthy first-degree relatives of type 2 diabetics. Aging Cell 21:e13557
Stout MB, Justice JN, Nicklas BJ, Kirkland JL (2017) Physiological aging: links among adipose tissue dysfunction, diabetes, and frailty. Physiology (bethesda) 32:9–19
Tabula Muris C (2020) A single-cell transcriptomic atlas characterizes ageing tissues in the mouse. Nature 583:590–595
Tchkonia T, Kirkland JL (2018) Aging, cell senescence, and chronic disease: emerging therapeutic strategies. JAMA 320:1319–1320
Tchkonia T, Morbeck DE, von Zglinicki T, van Deursen J, Lustgarten J, Scrable H, Khosla S, Jensen MD, Kirkland JL (2010) Fat tissue, aging, and cellular senescence. Aging Cell 9:667–684
Topol LZ, Bardot B, Zhang Q, Resau J, Huillard E, Marx M, Calothy G, Blair DG (2000) Biosynthesis, post-translation modification, and functional characterization of Drm/Gremlin. J Biol Chem 275:8785–8793
Tsiloulis T, Watt MJ (2015) Exercise and the regulation of adipose tissue metabolism. Prog Mol Biol Transl Sci 135:175–201
Turer AT, Scherer PE (2012) Adiponectin: mechanistic insights and clinical implications. Diabetologia 55:2319–2326
Van Deursen JM (2014) The role of senescent cells in ageing. Nature 509(439–46):46
Vergoni B, Cornejo PJ, Gilleron J, Djedaini M, Ceppo F, Jacquel A, Bouget G, Ginet C, Gonzalez T, Maillet J, Dhennin V, Verbanck M, Auberger P, Froguel P, Tanti JF, Cormont M (2016) DNA damage and the activation of the p53 pathway mediate alterations in metabolic and secretory functions of adipocytes. Diabetes 65:3062–3074
Villaret A, Galitzky J, Decaunes P, Esteve D, Marques MA, Sengenes C, Chiotasso P, Tchkonia T, Lafontan M, Kirkland JL, Bouloumie A (2010) Adipose tissue endothelial cells from obese human subjects: differences among depots in angiogenic, metabolic, and inflammatory gene expression and cellular senescence. Diabetes 59:2755–2763
Weisberg SP, Mccann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW Jr (2003) Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest 112:1796–1808
Weyer C, Foley JE, Bogardus C, Tataranni PA, Pratley RE (2000) Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance. Diabetologia 43:1498–1506
Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, Troy AE, McKeon C, Darlington GJ, Spiegelman BM (1999) Cross-regulation of C/EBP alpha and PPAR gamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell 3:151–158
Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, Sole J, Nichols A, Ross JS, Tartaglia LA, Chen H (2003) Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 112:1821–1830
Xu M, Palmer AK, Ding H, Weivoda MM, Pirtskhalava T, White TA, Sepe A, Johnson KO, Stout MB, Giorgadze N, Jensen MD, Lebrasseur NK, Tchkonia T, Kirkland JL (2015a) Targeting senescent cells enhances adipogenesis and metabolic function in old age. Elife 4:e12997
Xu M, Tchkonia T, Ding H, Ogrodnik M, Lubbers ER, Pirtskhalava T, White TA, Johnson KO, Stout MB, Mezera V, Giorgadze N, Jensen MD, Lebrasseur NK, Kirkland JL (2015b) JAK inhibition alleviates the cellular senescence-associated secretory phenotype and frailty in old age. Proc Natl Acad Sci 112:E6301–E6310
Yang J, Eliasson B, Smith U, Cushman SW, Sherman AS (2012) The size of large adipose cells is a predictor of insulin resistance in first-degree relatives of type 2 diabetic patients. Obesity (silver Spring) 20:932–938
Ye RZ, Richard G, Gevry N, Tchernof A, Carpentier AC (2022) Fat cell size: measurement methods, pathophysiological origins, and relationships with metabolic dysregulations. Endocr Rev 43:35–60
Zaragosi LE, Wdziekonski B, Villageois P, Keophiphath M, Maumus M, Tchkonia T, Bourlier V, Mohsen-Kanson T, Ladoux A, Elabd C, Scheideler M, Trajanoski Z, Takashima Y, Amri EZ, Lacasa D, Sengenes C, Ailhaud G, Clement K, Bouloumie A, Kirkland JL, Dani C (2010) Activin a plays a critical role in proliferation and differentiation of human adipose progenitors. Diabetes 59:2513–2521
Zatterale F, Longo M, Naderi J, Raciti GA, Desiderio A, Miele C, Beguinot F (2019) Chronic adipose tissue inflammation linking obesity to insulin resistance and type 2 diabetes. Front Physiol 10:1607
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We acknowledge financial support from the Knut and Alice Wallenberg Foundation (2020.0118), the Swedish Research Council (202200688), the Novo Nordisk Foundation (0078138), the Heart and Lung Foundation (20220355) and the Swedish Diabetes Research Foundation (DIA2022-714) to U.S.
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Nerstedt, A., Smith, U. The impact of cellular senescence in human adipose tissue. J. Cell Commun. Signal. 17, 563–573 (2023). https://doi.org/10.1007/s12079-023-00769-4
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DOI: https://doi.org/10.1007/s12079-023-00769-4