Abstract
Purpose
Leishmaniasis, caused by the parasite of the genus Leishmania, is a neglected tropical disease which is endemic in more than 60 countries. In South-East Asia, Brazil, and East Africa, it mainly occurs as kala-azar (visceral leishmaniasis, VL), and subsequently as post kala-azar dermal leishmaniasis (PKDL) in a smaller portion of cases. As stated per WHO roadmap, accessibility to accurate diagnostic methods is an essential step to achieve elimination. This study aimed to test the accuracy of a portable minoo device, a small battery-driven, multi-use fluorimeter operating with isothermal technology for molecular diagnosis of VL and PKDL.
Methods
Fluorescence data measured by the device within 20 min are reported back to the mobile application (or app) via Bluetooth and onward via the internet to a backend. This allows anonymous analysis and storage of the test data. The test result is immediately returned to the app displaying it to the user.
Results
The limit of detection was 11.2 genome copies (95% CI) as determined by screening a tenfold dilution range of whole Leishmania donovani genomes using isothermal recombinase polymerase amplification (RPA). Pathogens considered for differential diagnosis were tested and no cross-reactivity was observed. For its diagnostic performance, DNA extracted from 170 VL and PKDL cases, comprising peripheral blood samples (VL, n = 96) and skin biopsies (PKDL, n = 74) from India (n = 108) and Bangladesh (n = 62), was screened. Clinical sensitivity and specificity were 88% and 91%, respectively.
Conclusion
Minoo devices can offer a convenient, cheaper alternative to other molecular diagnostics. Its easy handling makes it ideal for use in low-resource settings to identify parasite burden.
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Introduction
Worldwide, new emerging diseases are a threat to public health systems. Low- and middle-income countries (LMIC) are facing the additional burden of ancient pathogens, circulating in human and animal populations. To control these diseases, the World Health Organization (WHO) created a list of neglected tropical diseases (NTDs) together with a program to eliminate these pathogens targeted for 2030 [1, 2]. One of the NTDs is Leishmaniasis, caused by the protozoan parasite Leishmania spp. It is strongly associated with poverty and/or rural settings [3,4,5]. Transmission between mammals occurs via blood-feeding female phlebotomine sandflies (Diptera, Psychodidae) [6].
Leishmaniasis has a range of clinical manifestations that include systemic involvement (visceral leishmaniasis, VL), along with various forms of dermal leishmaniasis and mucocutaneous leishmaniasis. In South-East Asia and East Africa, the causative species of VL is Leishmania donovani (L. donovani), while in the Mediterranean region and the Americas, it is Leishmania infantum (also known as Leishmania chagasi) [7]. The clinical symptoms for VL include weight loss, intermittent fever, pancytopenia, and hepatosplenomegaly [8]. In India, 2.5–20% of treated VL cases develop a dermal sequela termed as post kala-azar dermal leishmaniasis (PKDL) [9,10,11]. These patients are epidemiologically important as the parasites are present in the dermis which makes them accessible for ingestion by sandflies as part of the blood meal. Therefore, these patients serve as a reservoir for disease transmission [12,13,14,15].
In 2005, WHO published the first global and regional strategies to prevent, control, and eliminate kala-azar in India, Bangladesh, and Nepal as a public health problem. The key strategies toward elimination of VL encompass early diagnosis and complete case management; integrated vector management and vector surveillance; effective disease surveillance through passive and active case detection; social mobilization and building partnerships; and implementation and operational research [16]. The laboratory diagnosis of kala-azar is usually performed by microscopic observation of intracellular amastigotes (Leishman Donovan bodies) in spleen or bone marrow aspirates. Since this method is invasive, serological tests such as the rK39 strip test are preferred as primary diagnostic method [5]. For PKDL, the rK39 strip test falters in terms of specificity, as positivity may be attributable to a previous VL infection with presence of circulating anti-leishmanial antibodies [17]. Therefore, PKDL cases are diagnosed empirically based on clinical suspicion followed by detection of L. donovani parasites in slit skin smears or skin biopsies [18].
Clinical presentations for PKDL include any combination of macules, papules and/or nodules termed polymorphic PKDL, or hypopigmented lesions termed macular PKDL. Even though the parasite load is significantly lower in macular lesions [19], both manifestations comprise an equal proportion of the disease burden, are infectious to sandflies [14, 20], and therefore contribute to disease transmission [13]. Diagnosis of PKDL and monitoring of treatment are challenging, attributable to patients having poor health seeking behavior as the disease is not life-threatening [20, 21]. Furthermore, the low parasite load in macular PKDL cases leads to false negativity in slit skin smears, as the amastigotes are difficult to observe microscopically [22, 23]. In addition, these macular PKDL lesions can closely mimic leprosy, vitiligo, pityriasis versicolor, and psoriasis, thereby presenting a diagnostic dilemma [24].
To ensure the success of the kala-azar elimination program in South-East Asia (KAEP), there is a need for an accurate, rapid, and practical detection method to diagnose VL and PKDL [25]. In terms of compliance of the patient to treatment, the time to result delivery plays an important role. In rural areas, medical care is not sufficient, and it takes time and financial support to get to a medical facility. As PKDL occurs in the marginalized sections of society, there is a need for rapid detection methods that could shorten the turnaround time from diagnosis to treatment [26].
Real-time qPCR is a highly sensitive and specific detection method for parasite DNA [27, 28], but usually requires trained personnel and large footprint laboratory equipment that is very challenging to be implemented in resource limited settings. Isothermal amplification techniques, e.g., Recombinase Polymerase Amplification (RPA) amplify DNA at a constant temperature with the use of much simpler equipment than qPCR. RPA is highly specific and sensitive making it a valuable tool for molecular detection in low-resource settings [29]. The devices typically used for RPA are portable and relatively small, but still limited to use in small medical facilities or in a mobile laboratory.
In this study, we assessed the diagnostic potential of the rapid, smartphone-based minoo device for human VL and PKDL using isothermal RPA, which can be utilized as a point-of-need test. The minoo is a small, battery-driven fluorometer with a thermal control system, measuring fluorescence signal during isothermal amplification. The sensitivity and specificity of minoo were determined under laboratory conditions and then with archived clinical samples.
Materials and methods
Ethical approval
The study was approved by the Institutional Ethics Committee of Institute of Post Graduate Medical Education and Research IPGMER, Kolkata, India (Approval ID: IPGME&R/IEC/2021/273) and the institutional review board (IRB) committee of International Centre for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh (No: PR-14093). All study participants or their legally accepted guardian provided written informed consent.
Study population
In India, archived samples previously used in an RPA-based study [30] were tested by the minoo device at IPGMER, Kolkata, India. In total, n = 36 extracted samples from whole blood and n = 60 from skin biopsies were included. The samples from Bangladesh were archived samples at the icddr,b, Dhaka, Bangladesh. Here DNA extracts from whole blood, n = 72 [31] and skin-biopsy samples, n = 2 [32] were included.
Spin column-based DNA extraction method
In India, the QIAmp DNA mini kit (Qiagen, Hilden, Germany) was used for DNA extraction from 200 µL heparinized blood of patients suspected/diagnosed with VL and from skin biopsies in phosphate-buffered saline (20 mM, pH 7.4, PBS) of PKDL suspected cases according to manufacturer’s instructions. After excising skin biopsies into small pieces, DNA was eluted in 50 µL of DNA elution buffer.
In Bangladesh, DNA was isolated from 200 μL of whole blood samples using the QIAamp DNA tissue and blood mini kit. DNA from skin samples was extracted using DNeasy Blood and Tissue Kits (Qiagen, Hilden, Germany) following the manufacturer’s instructions, with a minor modification: skin-biopsy materials were incubated at 37 °C overnight after addition of lysis buffer (ATL) and proteinase K. On the following day, the material was homogenized and incubated at 56 °C for 2 h before purification according to the manufacturer’s instructions. DNA was eluted in 200 µL PCR grade water or elution buffer. Extracted DNA samples were stored at − 20 °C until downstream analysis.
Real-time PCR
In India, real-time qPCR was undertaken using the ABI StepOnePlus Real-Time Thermal Cycler (Thermo Fisher Scientific, Waltham, USA) and performed using forward primer: 5′-CTTTTCTggTCCTCCgggTAgg-3′, reverse primer: 5′-CCACCCggCCCTATTTTACACCAA-3′ and TaqMan probe (5′-6FAM-TTTTCgCAgAACgCCCCTACCCgC—BBQ-3′) with the LightCycler Multiplex DNA Master (Roche, Basel, Switzerland). In a total volume of 20 µL, 15 µL of the reaction mixture (TaqMan Master Mix, containing 100 nM of each primer and 50 nM of probe) and 5 µL of DNA was used. The protocol included a heating step of 95 °C for 1 min, 45 cycles of a denaturation step at 95 °C for 10 s, and an annealing and extension step of 60 °C for 45 s. The last step was a cooling step at 40 °C for 30 s. The cutoff value was set at a cycle threshold (Ct) value > 30.
In Bangladesh, targeting conserved region of Leishmania RLEP repeats (L42486.1) specific for L. donovani and L. infantum, Taqman primers and probes were designed to perform the real-time PCR following a method described by Vallur et al. [33]. Briefly, a 20 μL reaction mix was prepared containing 9 μL template, 10 μL of TaqMan® Gene Expression Master Mix (2×), 1 μL pre-ordered primer–probe mix and PCR grade water. Amplification was performed on a Biorad CFX96 iCycler system with following reaction conditions: 10 min at 95 °C, followed by 45 cycles of 15 s at 95 °C and 1 min at 60 °C. The total reaction time for real-time PCR was approximately 120 min. Specimens ran as duplicates. To quantify the parasite load, each run included one standard curve with DNA concentration corresponding to parasite load 10,000 to 0.1 parasites per reaction. Each run also included one reaction with molecular grade water as a negative control. Samples with cycle threshold (Ct) > 40 were considered as negative.
Isothermal DNA amplification
The method of detection in minoo devices was fluorescent signal measurement after isothermal DNA amplification. The TwistAmp Exo kit (TwistDx Ltd., Cambridge, UK) and Leishmania—RPA oligonucleotides (TibMolBiol, Berlin, Germany) were used as previously reported [34]. A total reaction volume of 50 µL was prepared using the TWIST Amp exo kit (Maidenhead, UK) that included rehydration buffer (29.5 µL), magnesium acetate (2.5 µL, final concentration 14 mM), 10 µM forward primer (2.1 µL), 10 µM reverse primer (2.1 µL), 10 µM probe (0.6 µL) (final concentrations primers 420 nM, probe 120 nM), and molecular nuclease free water (10.7 µL). All reagents were added into the lid of the reaction tube containing freeze-dried reaction pellets. The tube was closed, centrifuged, vortexed, and centrifuged again, then the content was transferred to a 200 µL PCR tube. Thereafter, 2.5 µL of template was added and mixed by pipetting. The tube was then incubated in the minoo device (midge medical GmbH, Berlin, Germany) for amplification at a constant temperature of 42 °C for 18 min.
Smartphone-based analysis
The minoo device is a portable diagnostic test station that facilitates the detection of biological reactants in a simplified and robust manner. The onboard battery is chargeable via USB port, one charge being sufficient for six independent runs, thus providing independence from unstable power supply. It includes an optical unit and a thermal control system to conduct and monitor isothermal amplification reactions in standard 200 µL PCR tubes. The thermal system incorporates a coil and onboard temperature sensors, which are strategically positioned near the sample for precise temperature control. The optical system of the device is based on a Light Emitting Diode (LED) and an onboard multichannel digital spectrometer.
The minoo test station is wirelessly (Bluetooth) controlled from an authorized application, which communicates the relevant data to the backend via the internet (Wi-Fi) for analysis and usage monitoring. Kassandra, a proprietary Python-based algorithm, is utilized to analyze the fluorescence amplification signal. Kassandra performs three main steps: data processing, where the background from the primary channel is removed and quality checks are performed; feature extraction, where relevant feature values are extracted from the amplification curve to differentiate positive and negative results; and classification, where a test result is calculated based on empirically defined thresholds and weights. After analysis, the result of the test (positive, negative, or inconclusive) is directly displayed on the smartphone application (See Fig. 1).
Molecular standards and limit of detection (LOD)
Whole genome DNA from a Leishmania donovani strain MHOM/IN/80/DD8 (WHO collaborating centre for leishmaniasis, Laboratorio de Referencia e Investigación en Parasitología Centro Nacional de Microbiología, Instituto De Salud Carlos III, Spain) and kinetoplast DNA standard (kDNA) (GeneArt, Regensburg, Germany) were used to determine the sensitivity of the assay (supplement 1). Tenfold serial dilutions ranging from 107 to 100 DNA molecules/µL were prepared and used to determine the assay’s limit of detection.
Pathogens for detection of cross-reactivity and analytical specificity
To determine the analytical specificity, nucleic acids of 12 pathogens, including different Leishmania species, were tested with the minoo assay (Table 1). All Leishmania species and Trypanosoma cruzi were provided by Laboratorio de Referencia en Leishmaniasis WHO Collaboration Centre for Leishmaniasis, Centro Nacional de Microbiología, Instituto De Salud Carlos III, Spain. Mycobacterium tuberculosis and Mycobacterium leprae were provided by Quality Control for Molecular Diagnostics (QCMD), Glasgow, United Kingdom and IPGMER, Kolkata, India, respectively. Plasmodium malariae and Monkeypox virus were contributed by the Robert Koch Institute, Berlin, Germany, and the German Primate Center, Göttingen, Germany, respectively.
Statistical analysis
To determine the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV), standard formulas were used [35]. RStudio version 1.3.1093 (RStudio, Boston, MA, United States) (64) was used to perform probit regression and calculation of the LOD. Data were visualized using the ggplot2 package (v3.3.3; [36]).
Results
Diagnostic performance of Leishmania-RPA on the minoo device
Analytical sensitivity and specificity
The LOD was determined using a tenfold dilution of a whole genome L. donovani standard and the synthetic molecular kDNA (kinetoplast DNA) standard. The LOD was 11.2 copies/reaction (95% CI) of the whole genome (Fig. 1a) and 134 copies/reaction (95% CI) of the kDNA standard (Fig. 1b). The reaction time was 18 min, with a preparation time of approximately 5 min per sample.
All tested Leishmania species were detected. No cross-reactivity was observed with nucleic acids from other pathogens (Table 1; Fig. 2).
Clinical samples
In total, 170 human samples (blood: n = 96; skin biopsies: n = 74) were screened by minoo in India and Bangladesh. Of these samples, 94/170 were positive by real-time qPCR and 83/94 were detected positive by minoo, 76/170 were detected negative by qPCR and 69/76 were detected negative by minoo (Fig. 3). This yields to an overall sensitivity and specificity of the RPA on the minoo across all samples of 88% and 91%, respectively (Table 2). The area under the receiver operating characteristic curve (AUC value) for VL was found to be 0.91 (95% CI 0.8466–0.9761) and for PKDL, it was 0.87 (95% CI 0.7885–0.9645) (Figs. 4 and 5). An inverse relationship between the Ct value in qPCR and the sensitivity of minoo in detecting Leishmania DNA was found (Table 3).
Discussion
The WHO Elimination Program for NTDs is gradually approaching their goal since the development of the first NTD roadmap for prevention and control [2]. However, more than 1 billion people worldwide still suffer from the burden of NTDs. Accurate, simple, and cost-effective diagnostic tools are essential to reach and maintain the goals of prevention, control, and elimination of these pathogens. In the roadmap for Neglected Tropical Diseases 2021–2030, the WHO emphasizes the need for innovative molecular diagnostic approaches to achieve these goals [2]. A WHO-working group—the Diagnostic Technical Advisory Group for Neglected Tropical Diseases (DTAG) is responsible for identifying and emphasizing diagnostic needs. In India, NVBDCP and the Kala-azar Task Force are involved in these efforts. For countries like Nepal and Bangladesh, which have already achieved elimination status, it is crucial to continue their efforts of surveillance and prevention [37].
In this study, we evaluated the efficacy of a portable device that enables molecular point-of-need diagnostics. This is highly relevant for monitoring of VL cases as there is no rapid antigen-based test available for the detection of the parasites in peripheral blood. Serological antibody tests (like the rK39 strip test) falter in terms of sensitivity—especially in patients with immunodeficiency or HIV infections [38]. In addition, serological assays do not distinguish between active and past infections, which might lead to false positive results [39,40,41]. Moreover, there is a problem of cross-reactivity with antibodies from other parasitic infections like Chagas and mycobacterial infections like Leprosy or tuberculosis [42,43,44]. The analytical sensitivity of the Leishmania-RPA evaluated on the minoo device (11.2 genome copies/reaction) suggests its suitability for the detection of parasites in PKDL lesions, as the mean parasite load in skin biopsies is approximately 50 per µg genomic DNA, with higher load in polymorphic cases and a lower load in macular cases [32].
For VL patients, the parasite load in the blood varies widely between 39 parasites/mL and 2.16 × 105 parasites/mL [45]. The clinical sensitivity and specificity of the minoo assay were 88% and 91%, respectively. In regard to the false negative results, several aspects can be considered. As there is a relation between Ct values of real-time qPCR and false negative samples in minoo, low parasite load (especially in VL samples) could be the cause. However, as the number of samples tested varies between the different Ct ranges, they cannot be directly compared. Another impact is the storage time of the extracted samples. Especially in Bangladesh, high Ct values of the samples were observed. All of them were archived samples that had previously been used for other studies, stored at − 20 °C. It is known that the process of freezing and thawing influences the integrity of nucleic acids which could then impact the amplification profiles [46,47,48]. Therefore, it is likely that the use of fresh samples would significantly improve the sensitivity [49].
While molecular testing usually is confined to a well-equipped laboratory, recent advancements make the development of portable tests possible [50]. For the diagnosis of VL, Recombinase Polymerase Amplification (RPA) has proven to be a reliable diagnostic method [30, 34]. However, these assays used a more expensive device that, although portable, was still tied to the use of a suitcase laboratory. The major advantage of the minoo device is that it has been designed with usability at the core of the design which in principle allows on-site testing by a relatively inexperienced lay user. Data transmission to the backend did not suffer from internet connection instabilities experienced during testing and all data sets were recorded in full on the backend. Lyophilized RPA reagents are cold-chain independent, and oligonucleotide containing lyopellets additionally simplifies the protocol [51]. With this, the minoo assay addresses seven of the REASSURED criteria for diagnostics (real-time connectivity, affordable, sensitive, specific, user-friendly, rapid and robust, and deliverable to end users), but in the case of leishmaniasis, testing does not yet cover ease of specimen collection and the category “equipment-free” [52]. However, the rechargeable device is an alternative to platforms which are nominally described as equipment-free, but actually use a one-use only disposable device [53].
Furthermore, different features (e.g., temperature) of the minoo devices can be easily adapted, allowing the use of other RPA assays for different pathogens. This is especially important in areas with a high prevalence of multiple diseases. Here, it is essential to focus on widespread diagnostic tools instead of disease-specific diagnostics [2].
Another advantage of this platform is the digital/cloud-based interface, which could enable transmission of results to Ministry of Health (MOH) surveillance programs and facilitate data processing. Comprehensive databases are crucial for disease risk assessment and evaluation of containment measures [54]. Furthermore, NTDs are considered as tracers to reveal general healthcare shortages. Thus, a high prevalence of an NTD highlights the need for healthcare improvement [2]. Although the results are highly promising, the assay has some limitations. One critical point is the handling of sensitive data. To address this point, the Smartphone App generates an anonymous ID code for each test, and personal data are not handled on the backend, thus providing personal data protection. Possible transmission of data to other servers in the future would need to be set up according to local data protection regulations. Currently 95% of the world population have access to broadband (3G) mobile phone network coverage although some blind spots remain [55]. Therefore, in most cases, internet access through the Smartphone App should be possible.
For detection of Leishmania, blood or skin samples are the target samples. These invasive procedures limit Leishmania diagnostics to clinical facilities. However, VL can be detected from whole blood which might allow the use of capillary drop diagnostics (ongoing work). This would enable testing in a home-based setting. However, DNA extraction is the bottleneck of a simplified testing approach and needs to be addressed. The use of a rapid extraction method which has been used by previous studies for the extraction of DNA in tissue samples is a promising method to address this issue [56, 57].
Conclusion
The Leishmania-RPA on the minoo device has the potential to contribute to the progress of controlling the spread of Leishmania donovani and achieving/maintaining elimination status through its routine use in diagnosis and treatment monitoring for VL and PKDL. The smartphone-based result readout enables good applicability for use at the point-of-need. However, further studies need to be performed to confirm results and to address the bottleneck of sample collection and extraction. In addition, a multi-country clinical study is warranted prior to its broader point-of-need implementation in endemic settings.
Data availability
No datasets were generated or analysed during the current study.
References
Casulli A. New global targets for NTDs in the WHO roadmap 2021–2030. PLoS Negl Trop Dis. 2021;15: e0009373.
WHO. Ending the neglect to attain the Sustainable Development Goals: A road map for neglected tropical diseases 2021–2030. Geneva: WHO; 2021.
Okwor I, Uzonna J. Social and economic burden of human leishmaniasis. Am J Trop Med Hyg. 2016;94:489–93.
Calderon-Anyosa R, Galvez-Petzoldt C, Garcia PJ, Carcamo CP. Housing characteristics and leishmaniasis: a systematic review. Am J Trop Med Hyg. 2018;99:1547–54.
WHO. Leishmaniasis 2023. https://www.who.int/news-room/fact-sheets/detail/leishmaniasis.
Ready PD. Biology of phlebotomine sand flies as vectors of disease agents. Annu Rev Entomol. 2013;58:227–50.
Guerin PJ, Olliaro P, Sundar S, Boelaert M, Croft SL, Desjeux P, et al. Visceral leishmaniasis: current status of control, diagnosis, and treatment, and a proposed research and development agenda. Lancet Infect Dis. 2002;2:494–501.
Singh OP, Sundar S. Developments in diagnosis of visceral leishmaniasis in the elimination era. J Parasitol Res. 2015;2015: 239469.
Zijlstra E, Musa A, Khalil E, El Hassan I, El-Hassan A. Post-kala-azar dermal leishmaniasis. Lancet Infect Dis. 2003;3:87–98.
Kumar P, Chatterjee M, Das NK. Post kala-azar dermal leishmaniasis: clinical features and differential diagnosis. Indian J Dermatol. 2021;66:24–33.
Gedda MR, Singh B, Kumar D, Singh AK, Madhukar P, Upadhyay S, et al. Post kala-azar dermal leishmaniasis: a threat to elimination program. PLoS Negl Trop Dis. 2020;14: e0008221.
Ganguly S, Saha P, Chatterjee M, Roy S, Ghosh TK, Guha SK, et al. PKDL—a silent parasite pool for transmission of leishmaniasis in kala-azar endemic areas of Malda District, West Bengal, India. PLoS Negl Trop Dis. 2015;9: e0004138.
Molina R, Ghosh D, Carrillo E, Monnerat S, Bern C, Mondal D, et al. Infectivity of post-kala-azar dermal leishmaniasis patients to sand flies: revisiting a proof of concept in the context of the kala-azar elimination program in the Indian subcontinent. Clin Infect Dis. 2017;65:150–3.
Mondal D, Bern C, Ghosh D, Rashid M, Molina R, Chowdhury R, et al. Quantifying the infectiousness of post-kala-azar dermal leishmaniasis toward sand flies. Clin Infect Dis. 2019;69:251–8.
Thakur S, Joshi J, Kaur S. Leishmaniasis diagnosis: an update on the use of parasitological, immunological and molecular methods. J Parasit Dis. 2020;44:253–72.
Sundar S, Singh OP, Chakravarty J. Visceral leishmaniasis elimination targets in India, strategies for preventing resurgence. Expert Rev Anti Infect Ther. 2018;16:805–12.
Singh DP, Sundar S, Mohapatra TM. The rK39 strip test is non-predictor of clinical status for Kala-azar. BMC Res Notes. 2009;2:187.
Sundar S, Rai M. Laboratory diagnosis of visceral leishmaniasis. Clin Diagn Lab Immunol. 2002;9:951–8.
Sengupta R, Mukherjee S, Moulik S, Mitra S, Chaudhuri SJ, Das NK, et al. In-situ immune profile of polymorphic vs. macular Indian Post Kala-azar dermal leishmaniasis. Int J Parasitol Drugs Drug Resist. 2019;11:166–76.
Sengupta R, Chaudhuri SJ, Moulik S, Ghosh MK, Saha B, Das NK, et al. Active surveillance identified a neglected burden of macular cases of Post Kala-azar Dermal Leishmaniasis in West Bengal. PLoS Negl Trop Dis. 2019;13: e0007249.
Basher A, Nath P, Nabi SG, Selim S, Rahman MF, Sutradhar SR, et al. A study on health seeking behaviors of patients of post-kala-azar dermal leishmaniasis. BioMed Res Int. 2015;2015: 314543.
Adams ER, Versteeg I, Leeflang MM. Systematic review into diagnostics for Post-Kala-Azar Dermal Leishmaniasis (PKDL). J Trop Med. 2013;2013: 150746.
Cloots K, Singh OP, Singh AK, Van der Auwera G, Kumar P, Gedda MR, et al. Assessing L. donovani skin parasite load: a proof of concept study of a microbiopsy device in an Indian setting. Front Cell Infect Microbiol. 2021;11: 645121.
Roy S, Roy M, Nath S, Chaudhuri SJ, Ghosh MK, Mukherjee S, et al. Role of molecular approaches to distinguish post kala-azar dermal leishmaniasis from leprosy: a case study. Indian J Dermatol Venereol Leprol. 2023;89:298–300.
Selvapandiyan A, Croft SL, Rijal S, Nakhasi HL, Ganguly NK. Innovations for the elimination and control of visceral leishmaniasis. PLoS Negl Trop Dis. 2019;13: e0007616.
Pal B, Kumari S, Kaur M, Wadhwa P, Murti K, Kumar R, et al. Barriers to the effective management and prevention of post kala-azar dermal leishmaniasis (PKDL) in the Indian subcontinent. Med J Armed Forces India. 2023;79:500–5.
Singh S, Sivakumar R. Recent advances in the diagnosis of leishmaniasis. J Postgrad Med. 2003;49:55.
Hossain F, Ghosh P, Khan MAA, Duthie MS, Vallur AC, Picone A, et al. Real-time PCR in detection and quantitation of Leishmania donovani for the diagnosis of Visceral Leishmaniasis patients and the monitoring of their response to treatment. PLoS One. 2017;12: e0185606.
Lobato IM, O’Sullivan CK. Recombinase polymerase amplification: basics, applications and recent advances. Trends Anal Chem. 2018;98:19–35.
Roy M, Ceruti A, Kobialka RM, Roy S, Sarkar D, Wahed AAE, et al. Evaluation of recombinase polymerase amplification assay for monitoring parasite load in patients with kala-azar and post kala-azar dermal leishmaniasis. PLoS Negl Trop Dis. 2023;17: e0011231.
Hossain F, Picado A, Owen SI, Ghosh P, Chowdhury R, Maruf S, et al. Evaluation of Loopamp™ leishmania detection kit and leishmania antigen ELISA for post-elimination detection and management of visceral leishmaniasis in Bangladesh. Front Cell Infect Microbiol. 2021;11: 670759.
Ghosh P, Hasnain MG, Hossain F, Khan MAA, Chowdhury R, Faisal K, et al. Evaluation of real-time PCR for diagnosis of post-kala-azar dermal leishmaniasis in endemic foci of Bangladesh. Open Forum Infect Dis. 2018;5: ofy234.
Vallur AC, Duthie MS, Reinhart C, Tutterrow Y, Hamano S, Bhaskar KRH, et al. Biomarkers for intracellular pathogens: establishing tools as vaccine and therapeutic endpoints for visceral leishmaniasis. Clin Microbiol Infect. 2014;20:O374–83.
Mondal D, Ghosh P, Khan MAA, Hossain F, Böhlken-Fascher S, Matlashewski G, et al. Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay. Parasit Vectors. 2016;9:1–8.
Safari S, Baratloo A, Elfil M, Negida A. Evidence based emergency medicine part 2: positive and negative predictive values of diagnostic tests. Emerg (Tehran). 2015;3:87–8.
Hadley W. Ggplot2: Elegrant graphics for data analysis. New York: Springer; 2016.
Rijal S, Sundar S, Mondal D, Das P, Alvar J, Boelaert M. Eliminating visceral leishmaniasis in South Asia: the road ahead. BMJ. 2019;364: k5224.
Cota GF, de Sousa MR, Demarqui FN, Rabello A. The diagnostic accuracy of serologic and molecular methods for detecting visceral leishmaniasis in HIV infected patients: meta-analysis. PLoS Negl Trop Dis. 2012;6: e1665.
Al Borzi A, Rasouli M, Nademi Z, Kadivar M, Pourabbas B. Evaluation of rK39 strip test for the diagnosis of visceral leishmaniasis in infants. EMHJ-East Mediterr Health J. 2006;12:294–9.
Welch RJ, Anderson BL, Litwin CM. Rapid immunochromatographic strip test for detection of anti-K39 immunoglobulin G antibodies for diagnosis of visceral leishmaniasis. Clin Vaccine Immunol. 2008;15:1483–4.
Elmahallawy EK, Martínez AS, Rodriguez-Granger J, Hoyos-Mallecot Y, Agil A, Mari JMN, et al. Diagnosis of leishmaniasis. J Infect Dev Ctries. 2014;8:961–72.
Roffi J, Dedet J-P, Desjeux P, Garré M-T. Detection of circulating antibodies in cutaneous leishmaniasis by enzyme-linked immunosorbent assay (ELISA). Am J Trop Med Hyg. 1980;29:183–9.
Kar K. Serodiagnosis of leishmaniasis. Crit Rev Microbiol. 1995;21:123–52.
Chatterjee M, Jaffe CL, Sundar S, Basu D, Sen S, Mandal C. Diagnostic and prognostic potential of a competitive enzyme-linked immunosorbent assay for leishmaniasis in India. Clin Diagn Lab Immunol. 1999;6:550–4.
Verma S, Kumar R, Katara GK, Singh LC, Negi NS, Ramesh V, et al. Quantification of parasite load in clinical samples of leishmaniasis patients: IL-10 level correlates with parasite load in visceral leishmaniasis. PLoS One. 2010;5: e10107.
Mutter GL, Zahrieh D, Liu C, Neuberg D, Finkelstein D, Baker HE, et al. Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays. BMC Genom. 2004;5:1–7.
Botling J, Edlund K, Segersten U, Tahmasebpoor S, Engström M, Sundström M, et al. Impact of thawing on RNA integrity and gene expression analysis in fresh frozen tissue. Diagn Mol Pathol. 2009;18:44–52.
Abd El Wahed A, Patel P, Faye O, Thaloengsok S, Heidenreich D, Matangkasombut P, et al. Recombinase polymerase amplification assay for rapid diagnostics of dengue infection. PLoS One. 2015;10: e0129682.
Tong Y, Lemieux B, Kong H. Multiple strategies to improve sensitivity, speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection. BMC Biotechnol. 2011;11:50.
Alp A. Recent advances in molecular point of care tests. Mikrobiyol Bul. 2022;56:580–91.
McCoy AG, Miles TD, Bilodeau GJ, Woods P, Blomquist C, Martin FN, et al. Validation of a preformulated, field deployable, recombinase polymerase amplification assay for Phytophthora species. Plants (Basel). 2020;9:466.
Land KJ, Boeras DI, Chen X-S, Ramsay AR, Peeling RW. REASSURED diagnostics to inform disease control strategies, strengthen health systems and improve patient outcomes. Nat Microbiol. 2019;4:46–54.
BIOSCIENCES S. DNA is everywhere—Sherlock can detect it anywhere 2023 [cited 2023 04.10.23]. https://sherlock.bio/platforms/.
Hürlimann E, Schur N, Boutsika K, Stensgaard A-S, Laserna de Himpsl M, Ziegelbauer K, et al. Toward an open-access global database for mapping, control, and surveillance of neglected tropical diseases. PLoS Negl Trop Dis. 2011;5: e1404.
ITU. Mobile network coverage 2021 [cited 2023 28.09.23]. https://www.itu.int/itu-d/reports/statistics/2021/11/15/mobile-network-coverage/.
Gunaratna G, Manamperi A, Böhlken-Fascher S, Wickremasinge R, Gunawardena K, Yapa B, et al. Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka. Parasit Vectors. 2018;11:665.
Chowdhury R, Ghosh P, Khan MAA, Hossain F, Faisal K, Nath R, et al. Evaluation of rapid extraction methods coupled with a recombinase polymerase amplification assay for point-of-need diagnosis of post-kala-azar dermal leishmaniasis. Trop Med Infect Dis. 2020;5:95.
Acknowledgements
The authors would like to thank Dana Rüster and Mario Reinhardt for their technical support in the laboratory.
Funding
Open Access funding enabled and organized by Projekt DEAL. The project was funded by Bill and Melinda Gates Foundation via VALIDATE Network—Vaccine development for complex intracellular neglected pathogens (Investment ID: INV-048694).
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The manuscript was written through contributions of all authors. Conceptualization and methodology, RMK, AC, AAEW; validation, RMK, AAEW; formal analysis, RMK, MW, EG, JBA; investigation, RMK, AC, MR, SR, RC, PG, FH, AAEW; resources, AC, MR, SR, RC, PG, FH, JM; data curation, RMK; writing—original draft preparation, RMK, AAEW; writing—review and editing, all coauthors; visualization, RMK, EG, MW, JBA; supervision, AAEW, UT, MC, DM, MW; project administration, AAEW. All authors have given approval to the final version of the manuscript.
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Midge medical GmbH provided the minoo devices and test result data from the system backend.
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Kobialka, R.M., Ceruti, A., Roy, M. et al. Portable smartphone-based molecular test for rapid detection of Leishmania spp.. Infection 52, 1315–1324 (2024). https://doi.org/10.1007/s15010-024-02179-z
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DOI: https://doi.org/10.1007/s15010-024-02179-z