Abstract
Integrin αvβ6 is expressed at an undetectable level in normal tissues, but is remarkably upregulated during many pathological processes, especially in cancer and fibrosis. Noninvasive imaging of integrin αvβ6 expression using a radiotracer with favorable in vivo pharmacokinetics would facilitate disease diagnosis and therapy monitoring. Through disulfide-cyclized method, we synthesized in this study, a new integrin αvβ6-targeted cyclic peptide (denoted as cHK), and radiolabeled it with 99mTc. The ability of the resulting radiotracer 99mTc–HYNIC–cHK to detect integrin αvβ6 expression in pancreatic cancer xenografts and idiopathic pulmonary fibrosis was evaluated using small-animal single-photon emission computed tomography (SPECT)/computed tomography (CT). 99mTc–HYNIC–cHK showed significantly improved in vivo metabolic stability compared to the linear peptide-based radiotracer 99mTc–HYNIC–HK. 99mTc–HYNIC–cHK exhibited similar biodistribution properties to 99mTc–HYNIC–HK, but the tumor-to-muscle ratio was significantly increased (2.99 ± 0.87 vs. 1.82 ± 0.27, P < 0.05). High-contrast images of integrin αvβ6-positive tumors and bleomycin-induced fibrotic lungs were obtained by SPECT/CT imaging using 99mTc–HYNIC–cHK. Overall, our studies demonstrate that 99mTc–HYNIC–cHK is a promising SPECT radiotracer for the noninvasive imaging of integrin αvβ6 in living subjects.
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Introduction
Integrin αvβ6, a member of the integrin family, is present at undetectable levels in adult differentiated tissues, but is overexpressed during embryogenesis, tumorigenesis, and tissue injury (Breuss et al. 1993; Desgrosellier and Cheresh 2010; Peng et al. 2016). Increased expression of integrin αvβ6 usually correlates with more aggressive disease and poor prognosis (Bates and Mercurio 2005; Elayadi et al. 2007; Lee et al. 2006), and the upregulation of integrin αvβ6 expression in a wide variety of cancers is associated with increased tumor cells migration, invasion, and metastasis (Bates 2005; Bates and Mercurio 2005). In addition to caner, de novo or increased expression of integrin αvβ6 has also been observed in pathological process of fibrosis (Patsenker et al. 2008; Pi et al. 2015). Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fibrotic lung disease with a poor prognosis (Gribbin et al. 2006; Navaratnam et al. 2011; Wells 2013). Integrin αvβ6-mediated transforming growth factor (TGF)-β activation has been implicated in multiple models of lung fibrosis, and the upregulated expression of integrin αvβ6 has also been found in patients with IPF (Horan et al. 2008; Xu et al. 2009). Importantly, the expression of integrin αvβ6 is temporally and spatially associated with the course of the fibrosis progression (Puthawala et al. 2008).
Due to the critical role of integrin αvβ6 in tumorigenesis and fibrogenesis, it has emerged as an appealing target for diagnostic imaging, prognosis evaluation, and therapeutic responses monitoring (Agarwal 2014; Cantor et al. 2015; Saini et al. 2015; Yang et al. 2015). Therefore, noninvasive and quantitative imaging of integrin αvβ6 expression by molecular imaging techniques would be of great potential for better management of these diseases. Previous studies have focused on the development of positron emission tomography (PET) and single-photon emission computed tomography (SPECT) radiotracers for in vivo imaging of integrin αvβ6 expression (Hackel et al. 2013; Hausner et al. 2007, 2008, 2009a, b, 2013; Hu et al. 2014; John et al. 2013; Kimura et al. 2012; Li et al. 2011; Liu et al. 2014b; Man et al. 2013; Nothelfer et al. 2009; Saha et al. 2010; Satpati et al. 2014; Singh et al. 2014; Ueda et al. 2014; Zhu et al. 2014). Although promising, most of these radiotracers are based on linear peptides, which have poor in vivo metabolic stability and suboptimal pharmacokinetics. For example, we observed recently that a 99mTc-labeled linear peptide (RGDLATLRQLAQEDGVVGVRK, the HK peptide) completely degraded in vivo within 30 min after injection, leading to a very low tumor uptake and tumor-to-nontumor ratios (Liu et al. 2014b).
Peptide cyclization has been reported to be a powerful strategy to improve the stability of peptides by means of adopted resistance to enzymatic degradation (Besser et al. 2000; Bogdanowich-Knipp et al. 1999a, b; Gilon et al. 1991; Pakkala et al. 2007; Roxin and Zheng 2012; Shi et al. 2016). To overcome the limitations of 99mTc-labeled HK peptide, in this study, we selected the first seven amino acid residues of the integrin αvβ6-targeting HK peptide and added a lysine residue at C-terminal in order to conjugate the chelator. We then cyclized it by adding a cysteine residue at N- and C-terminals, respectively, to generate a new peptide c (CRGDLATLKC, denoted as cHK). The peptide cHK was conjugated with the chelator sodium succinimidyl 6-(2-(2-sulfonatobenzaldehyde) hydrazono) nicotinate (HYNIC)-NHS and then radiolabeled with 99mTc. The resulting radiotracer 99mTc–HYNIC–cHK was evaluated in vivo as a SPECT radiotracer for imaging of integrin αvβ6 expression in both cancer and IPF mouse models.
Results
Chemistry and radiochemistry
The Fmoc-cHK–HYNIC conjugate (Fig. 1A) was prepared by direct conjugation of Fmoc-cHK peptide with HYNIC-NHS. After the removal of Fmoc group, the final product HYNIC–cHK was confirmed by high-performance liquid chromatography (HPLC) and mass spectrometry. The HPLC purity of HYNIC–cHK was >95% before being used for 99mTc radiolabeling. The 99mTc-labeling procedure was done within 30 min with a yield ranging from 85% to 90%. The radiochemical purity was >95% after purification, and the specific activity was >30 MBq/nmol.
Cell-binding assay
Similar to the HK peptide, cHK peptide also inhibited the binding of 125I–RGDLATLRQLAQEDGVVGVRYK (HYK) to integrin αvβ6-expressing BxPC-3 cells in a concentration-dependent manner, but the integrin αvβ6 binding affinity of cHK was lower compared to that of HK peptide. The IC50 values for cHK and HK were 20.25 ± 1.17 and 3.55 ± 0.09 nmol/L, respectively (Fig. 1B).
The binding specificity of 99mTc–HYNIC–cHK to integrin αvβ6 was evaluated in integrin αvβ6-positive BxPC-3 cells. As shown in Fig. 1C, the binding of 99mTc–HYNIC–cHK to BxPC-3 cells was significantly inhibited by the addition of excess doses of the cHK and HK peptides (from 0.79 ± 0.01 %AD to 0.03 ± 0.005 and 0.06 ± 0.007 %AD, respectively, P < 0.001).
Solution and metabolic stability
The in vitro solution stability of 99mTc–HYNIC–cHK in fetal bovine serum (FBS) or l-cysteine was monitored by radio-HPLC. Figure 2A shows that 99mTc–HYNIC–cHK remains stable for more than 4 h both in FBS and in the presence of l-cysteine.
We performed the metabolism studies of 99mTc–HYNIC–cHK using normal BALB/c mice. We analyzed the samples from both blood and urine to determine whether the radiotracer retains its chemical integrity at 0.5 h and 1 h postinjection. Figures 2B–F illustrate the radio-HPLC chromatograms of 99mTc–HYNIC–cHK before injection (Fig. 2B), in the blood (Fig. 2C, E) and in the urine (Fig. 2D, F). 99mTc–HYNIC–cHK retained its integrity in urine, while showing the degrees of metabolism to be 9.94% and 30.09% in blood at 0.5 h and 1 h postinjection, respectively. Compared to the linear peptide-based radiotracer 99mTc–HYNIC–HK (Liu et al. 2014b), 99mTc–HYNIC–cHK is much more stable in vivo.
Biodistribution
As shown in Fig. 3A, the uptake values of 99mTc–HYNIC–cHK in BxPC-3 tumors were 0.63 ± 0.18, 0.43 ± 0.09, and 0.33 ± 0.16 %ID/g at 0.5, 1, and 2 h after injection, respectively. The tumor uptake of 99mTc–HYNIC–cHK was higher than that in most of the normal organs, such as heart, liver, pancreas, bone, and muscle, at almost all time points examined (P < 0.05). The tumor uptake of 99mTc–HYNIC–cHK was significantly reduced with a coinjection of an excess dose of the cold HK peptide at 1 h after injection (from 0.43 ± 0.09 to 0.24 ± 0.04 %ID/g, n = 4, P < 0.01).
The uptake of 99mTc–HYNIC–cHK was similar to 99mTc–HYNIC–HK in BxPC-3 tumors at 0.5 h after injection (0.63 ± 0.18 vs. 0.58 ± 0.09, n = 4, P > 0.05). However, the uptake of 99mTc–HYNIC–cHK in muscle or bone was much lower, and the tumor-to-muscle (T/M) ratio was significantly higher than that of 99mTc–HHK (2.99 ± 0.87 vs. 1.82 ± 0.27, n = 4, P < 0.05; Fig. 3B).
SPECT imaging of BxPC-3 cancer xenografts
Representative small-animal SPECT/CT images of BxPC-3 tumor-bearing mice at 0.5 h and 1 h after intravenous injection of 99mTc–HYNIC–cHK are shown in Fig. 4A. The radiotracer showed clear tumor imaging with high contrast to the contralateral background. The in vivo receptor-binding property of 99mTc–HYNIC–cHK was determined by the blocking study. The tumor uptake of 99mTc–HYNIC–cHK was almost completely inhibited in the HK blocking group (P < 0.001; Fig. 4B, C).
SPECT/CT imaging of bleomycin-induced pulmonary fibrosis
As shown in Fig. 5A, markedly gray regions were observed by CT imaging in the lung areas of mice in the bleomycin (BLM)-treated mice, suggesting the evident fibrosis formation induced by BLM. Notably, an evident accumulation of 99mTc–HYNIC–cHK in the lungs of the BLM-treated mice was observed. In contrast, no significant uptake of 99mTc–HYNIC–cHK was observed in the phosphate-buffered saline (PBS)-treated mice (Fig. 5A, B). After the SPECT/CT imaging, the mice were sacrificed, and the presence of fibrosis in the edge of pulmonary lobes were verified by anatomic visualization after dissection in the BLM group. The hematoxylin–eosin (H&E) and Sirius red (specific for collagen) staining further confirmed the SPECT/CT findings (Fig. 5C).
Discussion
Overexpression of integrin αvβ6 has been found in approximately 100% of pancreatic cancers (Liu et al. 2014a). Integrin αvβ6-targeted imaging for pancreatic cancer detection and staging would contribute to improve the prospect of curing or controlling pancreatic cancer. We previously synthesized a linear peptide-based SPECT radiotracer (99mTc–HYNIC–HK) and demonstrated its potential for the specific detection of subcutaneous pancreatic tumor xenografts and liver metastases in mouse models (Liu et al. 2014b). However, 99mTc–HYNIC–HK had a very poor in vivo stability, which may significantly hamper its potential clinical translation. There are several approaches to improve the in vivo stability of peptides, including changing some amino acids of the peptide into D-amino acids, cyclizing the peptide to be a cyclic peptide, or engineering the peptide into scaffold-based peptides, such as cysteine knot (Zhu et al. 2014). In this study, we synthesized a cyclic peptide based on the HK peptide, and radiolabeled it with 99mTc, the resulting radiotracer 99mTc–HYNIC–cHK was evaluated both in vitro and in vivo.
Through the in vitro solution stability study, 99mTc–HYNIC–cHK was demonstrated to be rather stable in FBS or l-cysteine over 4 h. The in vivo metabolic study indicated that the stability in blood was considerably improved after cyclization (Fig. 2). Afterward, the integrin αvβ6-targeting ability of 99mTc–HYNIC–cHK was evaluated through cell-binding assays in integrin αvβ6-positive BxPC-3 cells. Similar to the HK peptide, cHK could also inhibit the binding of 125I–HYK on BxPC-3 cells in a dose-dependent manner. However, the binding affinity of cHK to integrin αvβ6 was slightly lower than that of the HK peptide. The decreased affinity may result from the shortened peptide sequence and constrained conformation of the cyclic peptide compared to the linear peptide. 99mTc–HYNIC–cHK retains the integrin αvβ6-targeting capability as evidenced by the significantly inhibited binding by adding an excess of cold cHK or HK peptide (Fig. 1C).
The in vivo integrin αvβ6-targeting specificity of 99mTc–HYNIC–cHK was confirmed by the biodistribution and SPECT/CT imaging studies in the BxPC-3 xenograft tumors. 99mTc–HYNIC–cHK exhibited rapid tumor accumulation and showed the maximum tumor-uptake values at 0.5 h after injection (Fig. 3A). Predominant kidney uptake of 99mTc–HYNIC–cHK was also observed, most likely due to the renal clearance of this radiotracer. The absolute tumor uptake of 99mTc–HYNIC–cHK was comparable to that of 99mTc–HHK at 0.5 h (Fig. 3). However, the tumor-to-muscle ratio was significantly higher for 99mTc–HYNIC–cHK compared to that of 99mTc-HHK, resulting in a favorable tumor imaging contrast.
In addition to cancer, de novo and increased expression of integrin αvβ6 also occur during fibrogenesis. The increased expression of integrin αvβ6 has been found in fibrotic lung tissue in patients with IPF and was demonstrated to play an important role in the progression of lung fibrotic disease in several different studies (Horan et al. 2008; Puthawala et al. 2008; Xu et al. 2009). To date, the only approach to detect the expression of integrin αvβ6 in the fibrotic lung is immunohistochemical analysis of biopsy samples (Raghu et al. 2011). This procedure is clinically impractical for many patients and suffers from sampling bias, resulting in incomplete information. Considering the high short-term mortality following lung biopsy (Utz et al. 2001), repeated sampling is unrealistic. Hence, noninvasive molecular imaging of integrin αvβ6 expression would offer a remarkable improvement for immunophenotyping patients with IPF. 18F–FDG and 68Ga-labeled somatostatin analogs targeting somatostatin receptor as PET tracers have been used for IPF stratification, but neither of these radiotracers targets well-validated pathways implicated in IPF (Ambrosini et al. 2010; Win et al. 2012). Cai et al. recently developed an optical activatable probe for noninvasive diagnosis of IPF by targeting matrix metalloproteinase type 2 (MMP-2), which was also correlated with IPF development. However, MMP-2 could be secreted into the blood stream, the nonspecific fluorescence signal recovery in tissues (e.g. liver) other than lung was noticed over time (Cai et al. 2013). John et al. developed an 111In-labeled αvβ6-specific peptide (111In–DTPA–A20FMDV2) as a SPECT radiotracer and for the first time used it for noninvasive measurement of integrin αvβ6 expression in lungs of mice with BLM-induced fibrosis (John et al. 2013). Their results showed that the lung uptake of 111In–DTPA–A20FMDV2 is quantifiable and correlates with the levels of αvβ6 protein, itgb6 messenger RNA, and hydroxyproline in the lungs.
The low uptake of 99mTc–HYNIC–cHK in normal lungs makes it a potential tool for quantitative and global analyses of integrin αvβ6 expression with high sensitivity in imaging the lung disorders. In the murine model of pulmonary fibrosis induced by BLM, a significant accumulating of 99mTc–HYNIC–cHK in lungs of BLM-treated mice was observed, compared with the PBS group (Fig. 5A, B). The H&E and Sirius red staining confirmed the lung fibrotic lesions in the lungs of BLM-treated mice (Fig. 5C). Compared with 111In, 99mTc is more suitable for labeling peptide-based probes because that the radioactive half-life of 99mTc matches the metabolic half-life of peptides. Moreover, 99mTc-labeled radiotracer is more widely available and cost effective. The high labeling yield of 99mTc chelator systems also allows the formulation of kits for the rapid preparation of radiotracers for widespread applications.
Although the metabolic stability of 99mTc–HYNIC–cHK after cyclization was significant improved compared to the linear radiotracer 99mTc–HYNIC–HK, the receptor-binding affinity of 99mTc–HYNIC–cHK was slightly decreased. In order to increase the receptor-binding affinity and further improve the in vivo pharmacokinetics of 99mTc–HYNIC–cHK, efforts such as polyethylene glycol (PEG)ylation and multimerization may be required to further optimize this radiotracer.
Conclusion
A cyclic peptide-based radiotracer 99mTc–HYNIC–cHK with improved in vivo metabolic stability was prepared and evaluated both in vitro and in vivo. 99mTc–HYNIC–cHK exhibited specific integrin αvβ6-targeting ability and was demonstrated to specific detection of integrin αvβ6 expression in subcutaneous pancreatic cancer xenografts and pulmonary fibrosis in animal models. Further optimization of 99mTc–HYNIC–cHK may eventually yield a clinical applicable radiotracer for SPECT imaging of integrin αvβ6 expression, disease staging, and monitoring of therapy efficacy.
Experimental section
Materials and reagents
All commercially available chemical reagents were used without further purification unless otherwise stated. The peptides Fmoc-cHK, HYK and HK were synthesized by ChinaPeptide Co., Ltd (Shanghai, China). Na99mTcO4 was obtained from a commercial 99Mo/99mTc generator (Beijing Atom High Tech Co., Beijing, China). The reversed-phase high-performance liquid chromatography (HPLC) system was Agilent Technologies 1260 Infinity HPLC (Agilent Technologies, Santa Clara, CA) coupled with the Raytest Gabi radioactivity detector (Raytest, Straubenhardt, Germany). Female BALB/c nude mice (4–5 weeks of age), BALB/c normal mice (4–5 weeks of age), and C57/BL6 mice (7–8 weeks of age) were purchased from Department of Laboratory Animal Science, Peking University Health Science Center (Beijing, China). BLM was purchased from Aladdin (Shanghai, China).
Synthesis of HYNIC-conjugated cHK peptide
The Fmoc-cHK peptide was conjugated with HYNIC–NHS using a standard procedure. Briefly, a solution of Fmoc-cHK was mixed with HYNIC–NHS at a mole ratio of 1:1.2. The pH was adjusted to 8.5–9.0 using N,N-Diisopropylethylamine. After stirring for 4 h at room temperature, the Fomc was removed by adding piperidine with a final volume fraction of 20%. The HYNIC–cHK was isolated by semi-preparative HPLC and lyophilized to afford the final product as a white powder (yield: 56%). Analytical HPLC (Retention time = 14.99 min) and mass spectrometry (MALDI-TOF–MS: m/z, 1380.66 for [MH]+ (C56H85N17O18S3, calculated molecular weight 1380.57) confirmed the identity of the product.
Preparation of 99mTc–HYNIC–cHK
For 99mTc labeling, a mixture of 20 mg tricine (100 mg/mL in 25 mmol/L succinate buffer, pH 5.0), 740 MBq (20 mCi) Na99mTcO4, and 30 μl SnCl2 (1 μg/μL in 0.1 mol/L HCl) was successively added to 20 μg of HYNIC–cHK with constant stirring at 99 °C for 10 min. Then 100 μl of ethylenediamine-N,N′-diacetic acid (100 mg/mL) was added to the mixture with contant stirring at 99 °C for 20 min. After allowing it to cool down to room temperature, the mixture was purified with Sep-Pak C18 cartridges (Waters) as preciously described (Jia et al. 2006).
Cell culture and animal models
The BxPC-3 human pancreatic cancer cell line was obtained from American Type Culture Collection. Cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37 °C in humidified atmosphere containing 5% CO2.
All animal experiments were performed in accordance with the guidelines of Peking University Animal Care and Use Committee. To establish the BxPC-3 subcutaneous tumor model, BxPC-3 cells (1 × 107 in 100 μl of PBS) were inoculated subcutaneously into the right front flanks of female BALB/c nude mice. The animals were used for in vivo studies when the tumor size reached 200–300 mm3 (3–4 weeks after inoculation). For the pulmonary fibrosis mouse model, BLM (1.5 units/kg, 50 μl in PBS) or PBS (50 μl; as a vehicle control) was administered once into the C57/BL6 mice by intratracheal injection. On day 21 (based on pilot studies), the mice with well-established pulmonary fibrosis were used for SPECT/CT imaging.
Integrin αvβ6 binding specificity
In vitro integrin αvβ6 binding affinities of cHK and HK were compared via displacement cell-binding assays (Dong et al. 2015) using 125I–HYK as the radioligand. 125I–HYK was prepared by labeling HYK with Na125I using the Iodogen method as previously reported (Liu et al. 2010). Experiments were performed on high integrin αvβ6-expressing BxPC-3 cells. The best-fit 50% inhibitory concentration (IC50) values were calculated by fitting the data with nonlinear regression using Graph-Pad Prism (GraphPad Software, Inc.).
In vitro integrin αvβ6 binding specificity of 99mTc–HYNIC–cHK was tested using the integrin αvβ6-positive BxPC-3 cells. Briefly, cells were seeded into 12-well plates and incubated overnight at 37 °C to allow adherence. After brief washing with PBS, tumor cells were incubated with 3.7 kBq 99mTc–HYNIC–cHK with or without an excess dose of cold cHK or HK peptide at 4 °C for 4 h. Tumor cells were then washed with chilled PBS and harvested by trypsinization with 0.05% trypsin. The cell suspensions were collected and measured in a γ-counter (Wallac 1470-002, Perkin-Elmer, Finland). The cell uptake was expressed as the percent added dose (%AD). Experiments were performed twice with triplicate samples.
Solution and metabolic stability
99mTc–HYNIC–cHK was incubated in FBS or l-cysteine (1.0 mg/mL) for 0, 0.5, 1, 2, and 4 h at 37 °C to test the in vitro solution stability. After passing through a 0.22-μm Millipore filter, the samples were analyzed by radio-HPLC.
The metabolic stability of 99mTc–HYNIC–cHK was evaluated in female BALB/c normal mice. Each mouse was administered with the radiotracer at a dose of 1 mCi in 100 μl saline via intravenous injection. At 0.5 h and 1 h postinjection, the blood and urine samples were collected. The samples were centrifuged at 8000 r/min for 15 min. The supernatant was collected, filtered through a 0.22-μm Millipore filter, and then analyzed by radio-HPLC.
Biodistribution
Biodistribution studies were performed using female BALB/c nude mice bearing BxPC-3 xenografts. Mice received an injection via the tail vein of 370 kBq (10 μCi) of 99mTc–HYNIC–cHK to evaluate the distribution of the radiotracer. The blocking experiments were also performed by coinjection of 99mTc–HYNIC–cHK with a saturating dose of the HK peptide (500 μg per mouse). At 0.5, 1, and 2 h after injection, the animals were sacrificed, and the tumors and the organs/tissues of interest were dissected and wet-weighed, and the radioactivity in the tissue was measured using a γ-counter. The results are presented as percentages of injected dose per gram of tissue (%ID/g). Values are expressed as mean ± SD (n = 4 per group).
Small-animal SPECT/CT imaging
Small-animal SPECT/CT scans of subcutaneous BxPC-3 tumor and pulmonary fibrosis mouse models were performed using a SPECT/CT system (NanoScan; Mediso, Budapest, Hungary). Each BxPC-3-bearing mouse was injected via tail vein with 37 MBq (1 mCi) of 99mTc–HYNIC–cHK. At 0.5 h and 1 h after injection, the mice were anesthetized by inhalation of 2% isoflurane and imaged using the Nano-SPECT/CT camera, The SPECT and CT fusion images were obtained using the automatic fusion software (InterView Fusion; Mediso Medical Imaging Systems, Budapest, Hungary).
Each BLM- or PBS-treated mouse was administered with 37 MBq of 99mTc–HYNIC–cHK via tail vein. After SPECT/CT imaging, the BLM- and PBS-treated mice were euthanized. Lungs were excised and fixed in 5% buffered formalin, embedded in paraffin, and cut into sections for staining with H&E or Sirius red as previously described (Yu et al. 2016).
Statistical analysis
Quantitative data were expressed as mean ± SD. Results were compared using the Student t test. P values of less than 0.05 were considered statistically significant.
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Acknowledgments
This work was partially supported by the National Key R&D Program of China (2018YFC1313300 and 2017YFA0205600), the National Natural Science Foundation of China (81471712, 81671747, 81630045, 81873907, and 81420108019), the Beijing Natural Science Foundation (L172007), the Beijing Nova Program Interdisciplinary Cooperation Project (Z181100006218136), and the Clinical Medicine Plus X-Young Scholars Project of Peking University (PKU2018LCXQ017).
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Hao Liu, Liquan Gao, Xinhe Yu, Lijun Zhong, Jiyun Shi, Bing Jia, Nan Li, Zhaofei Liu, and Fan Wang declare that they have no conflicts of interest.
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Liu, H., Gao, L., Yu, X. et al. Small-animal SPECT/CT imaging of cancer xenografts and pulmonary fibrosis using a 99mTc-labeled integrin αvβ6-targeting cyclic peptide with improved in vivo stability. Biophys Rep 4, 254–264 (2018). https://doi.org/10.1007/s41048-018-0071-1
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DOI: https://doi.org/10.1007/s41048-018-0071-1