Abstract
This study uses high performance liquid chromatography and capillary electrophoresis as analytical tools to evaluate flavonoids in hydrolyzed leaves extracts of Rubus erythrocladus Mart., Rosaceae, and Morus nigra L., Moraceae. For phytochemical analysis, the extracts were prepared by acid hydrolysis and ultrasonic bath and analyzed by high performance liquid chromatography using an ultraviolet detector and by capillary electrophoresis equipped with a diode-array detector. Quercetin and kaempferol were identified in these extracts. The analytical methods developed were validated and applied. Quercetin and kaempferol were quantified in R. erythrocladus, with 848.43 ± 66.68 μg g−1 and 304.35 ± 17.29 μg g−1,respectively, by HPLC-UV and quercetin, 836.37 ± 149.43 μg g−1, by CE-DAD. In M. nigra the quantifications of quercetin and kaempferol were 2323.90 ± 145.35 μg g−1and 1446.36 ± 59.00 μg g−1,respectively, by HPLC-UV and, 2552.82 ± 275.30 μg g−1and 1188.67 ± 99.21 μg g−1, respectively, by CEDAD. The extracts were also analyzed by ultra-performance liquid chromatography coupled with a diode-array detector and mass spectrometer (MS), UPLC-DAD/MS.
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Acknowledgments
We would like to thank CAPES, CNPq, PPGCF-UFRGS and Dra. Maria do Carmo Bassols Raseira (Embrapa). JASZ acknowledge CNPq for researcher fellowship.
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LRT contributed in sample preparation, chromatography and electrophoresis analysis, analysis of data and draft of the paper. GPRP contributed in sample preparation and chromatography analysis. ACOC and MS contributed in electrophoresis analysis and draft of the paper. SALB contributed to plants collections. AF and JASZ designed the study, lead and advised the laboratory work and performed the analysis of the data and drafted the manuscript.
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Tallini, L.R., Pedrazza, G.P.R., de Bordignon, S.A.L. et al. Analysis of flavonoids in Rubus erythrocladus and Morus nigra leaves extracts by liquid chromatography and capillary electrophoresis. Rev. Bras. Farmacogn. 25, 219–227 (2015). https://doi.org/10.1016/j.bjp.2015.04.003
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DOI: https://doi.org/10.1016/j.bjp.2015.04.003