Abstract
The present study was designed to investigate polyphenolic and sulphur contents of the aerial parts of Capparis spinosa var. aegyptia (Lam.) Boiss.. Capparaceae. wildly growing in Egypt. The chemical compositions of the water distilled essential oil were investigated by GC/MS analysis where the major constituent of the oil was methyl isothiocyanate (24.66%). Hydroethanolic extract was evaluated by LC-HRESI-MS-MS in both positive and negative modes. Forty-two compounds were identified including quercetin. kaempferol and isorhamnetin derivatives in addition to myricetin. eriodictyol. cirsimaritin and gallocatechin derivatives. Quercetin tetrahexoside dirhamnoside as well as kaempferol dihexo-side dirhamnoside have not been identified before in genus Capparis. Phenolic acids, such as quinic acid, p-coumaroyl quinic acid and chlorogenic acid were also identified. Evaluation of cytotoxic activity of hydroethanolic extract against three human cancer cell lines (MCF-7; breast adenocarcinoma cells. Hep-G2; hepatocellular carcinoma cells and HCT-116; colon carcinoma) using 3-4,5-dimethylthiazole-2-yl]-2.5-diphenyltetrazolium bromide (MTT) assay showed significant effect with IC50 values 24.5. 24.4 and 11 μg/ml. compared to Doxorubicin as a standard cytotoxic drug. C. spinosa revealed itself as a promising candidate for nutraceutical researches.
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Acknowledgments
The authors are thankful to Prof. Dr. Nahla Ayoub, professor of Pharmacognosy, Faculty of Pharmacy, British University in Egyptfor helping in LC–MS analysis.
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RO suggested the point, wrote the manuscript, carried out extraction procedures and interpreted LCMS analysis. MH interpreted the GC/MS data of essential oil and cytotoxic activity of HEE and revised the manuscript.
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Bakr, R.O., El Bishbishy, M.H. Profile of bioactive compounds of Capparis spinosa var. aegyptiaca growing in Egypt. Rev. Bras. Farmacogn. 26, 514–520 (2016). https://doi.org/10.1016/j.bjp.2016.04.001
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DOI: https://doi.org/10.1016/j.bjp.2016.04.001