Abstract
Hyptis carpinifolia Benth., Lamiaceae, is a species known popularly as “rosmaninho” and “mata-pasto”, and leaves are employed in Brazilian folk medicine to treat colds, flu, and rheumatism. The aim of this study was to perform a morphological description of H. carpinifolia and to evaluate the seasonal chemical variability of the leaf essential oils during 12 months. Macroscopic characterization of H. carpinifolia was carried out with the naked eye and with a stereoscopic microscope. Essential oils were isolated from leaves by hydrodistillation in Clevenger apparatus and analyzed by gas chromatography/mass spectrometry. Major compounds were found to be 1,8-cineole (39.6–61.8%), trans-cadina-1(6), 4-diene (2.8–17.5%), β-caryophyllene (4.4–10.0%), prenopsan-8-ol (4.2–9.6%) and β-pinene (2.9–5.3%). Results of essential oils compositions were processed by cluster analysis and principal component analysis. Data showed high variability in the concentration of the components. Besides, there was a seasonal variability of chemical composition, probably related mainly to the rainfall regime.
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Adams, R.P., 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th ed. Allured Publ. Corp, Carol Stream, IL.
Araújo, E.C.C, Silveira, E.R., Lima, M.A.S., Neto, M.A., De Andrade, I.L., Lima, M.A.A., 2003. Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth. J. Agric. Food Chem. 51, 3760–3762.
Balacs, T., 1997. Cineole-rich Eucalyptus. Int. J. Aromather. 8, 15–21.
Barros, F.M.C., Zambarda, E.O., Heinzmann, B.M., Mallmann, C.A., 2009. Variabilidade sazonal e biossíntese de terpenóides presentes no óleo essencial de Lippia alba (Mill.) N. E. Brown (Verbenaceae). Quim. Nova 32, 861–867.
Bordignon, S.A.L., 1990. O Gênero Hyptis Jacq. (Labiatae) no Rio Grande do Sul, Botânica. Universidade Federal do Rio Grande do Sul, Rio Grande do Sul.
Botrel, P.P., Pinto, J.E.B.P., Ferraz, V., Bertolucci, S.K.V., Figueiredo, F.C., 2010. Teor e composição química do óleo essencial de Hyptis marrubioides Epl., Lamiaceae em função da sazonalidade. Acta Sci. Agron. 32, 533–538.
Brandão, M.G.L., Pignal, M., Romaniuc, S., Grael, C.F.F., Fagg, C.W., 2012. Useful Brazilian plants listed in the field books of the French naturalist Auguste de Saint-Hilaire (1779-1853). J. Ethnopharmacol. 143, 488–500.
Caldas, G.R.F., Oliveira, A.R.S., Araujo, A.V., Quixabeira, D.C.A., Silva-Neto, J.C., CostaSilva, J.H., Menezes, I.R.A., Ferreira, F., Leite, A.C.L., Costa, J.G.M., Wanderley, A.G., 2014. Gastroprotective and ulcer healing effects of essential oil of Hyptis martiusii Benth. (Lamiaceae). PLOS ONE 9, 1–10.
Callegari-Jacques, S.M., 2003. Bioestatística: Princípios e aplicações. Editora Artmed S.A., São Paulo.
Corrêa, M.P., 1931. Dicionário das plantas úteis do Brasil e das exóticas cultivadas. Ministério da Agricultura, Rio de Janeiro.
Epling, C., 1949. Revisión del Género Hyptis (Labiatae). Rev. Del Museo de La Plata. Tomo VII, Sección Botânica, 153–497.
Franco, C.R.P., Alves, P.B., Andrade, D.M., Jesus, H.C.R., Silva, E.J.S., Santos, E.A.B., Antoniolli, A.R., Quintans-Júnior, L.J., 2011. Essential oil composition and variability in Hyptis fruticosa. Rev. Bras. Farmacogn. 21, 24–32.
Gobbo-Neto, L., Lopes, N.P., 2007. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Quim. Nova 30, 374–381.
Harley, R.M., 1988. Evolution and Distribution of Eriope (Labiatae) and its Relatives in Brasil. Academia Brasileira de Ciências, Rio de Janeiro.
Harley, R.M., 2012. Checklist and key of generaand species of the Lamiaceae of the Brazilian Amazon. Rodriguésia 63, 129–144.
Hussain, A.I., Anwar, F., Nigam, P.S., Sarker, S.D., Moore, J.E., Rao, J.R., 2011. Antibacterialactivity of some Lamiaceae essential oilsusing resazurinasan indicator of cellgrowth. LWT-Food Sci. Technol. 44, 1199–1206.
INMET, 2014. Instituto Nacional de Meteorologia. Ministério da agricultura, pecuária e abastecimento. http://www.inmet.gov.br/portal/index.php?r=bdmep/bdmep (accessed December 2015).
Jelani, S., Prabhakar, M., 1991. Farmacognostic study of leaf of Hyptis suaveolens L. Ancient Sci. Life X.I., 31–37.
Kuhnt, T.M., Probstle, A., Rimpler, H., Bauer, R., Heinrich, M., 1995. Biological and pharmacological activities and further constituints of Hyptis verticullata. Planta Med. 61, 227–232.
Lahlou, S., Interaminense, L.F., Leal-Cardoso, J.H., Duarte, G.P., 2003. Antihypertensive effects of the essential oil of Alpinia zerumbet and its main constituent, terpinen-4-ol, in DOCA-salt hypertensive conscious rats. Fundam. Clin. Pharmacol. 17, 323–330.
Martins, F.T., Santos, M.H., Polo, M., Barbosa, L.C.A., 2006. Variação química do óleo essencial de Hyptis suaveolens (L.) Poit. sob condições de cultivo. Quim. Nova 29, 1203–1209.
McNeil, M., Surfacey, P., Porter, R., 2011. Essential oils from the Hyptis genus - a review. Nat. Prod. Commun. 6, 1775–1796.
Mobot, 2016. Missouri Botanical Garden Tropicos. http://www.tropicos.org/Name/17600864 (accessed February 2016).
Morais, L.A.S., 2009. Influência dos fatores abióticos na composição química dos óleos essenciais. Hort. Bras. 27, S4050–S4063.
Moreira, A.C.P., Lima, E.O., Wanderley, P.A., Carmo, E.S., Souza, E.L., 2010. Chemical composition and antifungal activity of Hyptis suaveolens (L.) Poit leaves essential oil against Aspergillus species. Braz. J. Microbiol. 41, 28–33.
Moteki, H., Hibasami, H., Yamada, Y., Katsuzaki, H., Imai, K., Komiya, T., 2002. Specific induction of apoptosisby 1, 8-cineole in two human leukemia cell lines, but not a in human stomach cancer cell line. Oncol. Lett. 9, 757–760.
NIST, 1998. National Institute of Standards and Technology, PC Version of the NIST/EPA/NIH Mass Spectral Database. U.S. Department of Commerce, Gaithersburg.
Pignal, M., Romaniuc-Neto, S., De Souza, S., Chagnoux, S., Lange Canhos, D.A., 2013. Saint-Hilaire Virtual Herbarium: A New Upgradeable Tool to Study Brazilian Botany.
Pinto, N.V., Assreuy, A.M., Coelho-De-Souza, A.N., Ceccatto, V.M., Magalhães, P.J., Lahlou, S., Leal-Cardoso, J.H., 2009. Endothelium-dependent vasorelaxant effects of the essential oil fromaerial parts of Alpinia zerumbet and its main constituent 1, 8-cineole in rats. Phytomedicine 16, 1151–1155.
Rabei, S., El-Gazzar, A., 2007. Computer-generated keys to species of Hyptis s.l (Labiatae). Taeckholmia 27, 11–39.
Rebelo, M.M., Silva, J.K.R., Andrade, E.H.A., Maiav, J.G.S., 2009. Antioxidant capacity and biological activity of essential oil and methanol extract of Hyptis crenata Pohl ex Benth. Rev. Bras. Farmacogn. 19, 230–235.
Rodrigues, V.E.G., Carvalho, D.A., 2001. Levantamento etnobotânico de plantas medicinais no domínio do cerrado na região do alto Rio Grande - Minas Gerais. Cienc Agrotec. 25, 102–123.
Sales, J.F., Pinto, J.E.B.P., Ferri, P.H., Silva, F.G., Oliveira, C.B.A., 2009. Influência do nível de irradiância no crescimento, produção e composição química do óleo essencial de hortelã-do-campo (Hyptis marrubioides Epl.). Ciênc Agrotec. 30, 389–396.
Santos, F.A., Rao, V.S.N., 1998. Inflammatory edema induced by 1, 8-cineole in the hind paw of rats: a model for screening anti-allergic and anti-inflammatory compounds. Phytomedicine 5, 115–119.
Santos, F.A., Silva, R.M., Tome, A.R., Rao, V.S.N., Pompeu, M.M.L., Teixeira, M.J., 2001. 1, 8-cineole protects against liverfailure inanin-vivomurine model of endotoxemic shock. J. Pharm. Pharmacol. 53, 505–511.
Santos, M.R.V., Moreira, F.V., Fraga, B.P., De Sousa, D.P., Bonjardim, L.R., Quintans-Júnior, L.J., 2011. Cardiovascular effects of monoterpenes: a review. Rev. Bras. Farmacogn. 21, 764–771.
Silva, A.F., Barbosa, L.C.A., Nascimento, E., Casali, V.W.D., 2000. Chemical composition of the essential oil of Hyptis glomerata Mart, ex Schrank (Lamiaceae). J. Essent. Oil Res. 12, 725–727.
Silva, I.C., Moura, R.B., 2011. Espécies de Asteraceaee Lamiaceae usadas na medicina popular da região sudeste para problemas respiratórios: O que as evidências científicas indicam. Rev. Fitos. 6, 21–28.
I. Stat Soft, 2004. STATISTICA (data analysis software system), 7th ed.
Van Den Dool, H., Kratz, P.D., 1963. Generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr. 11, 463–471.
Vilela, G.R., Almeida, G.S., D’Arce, M.A.B.R., Moraes, M.H.D., Brito, J.O., Silva, M.F.G.F., 2009. Activity ofessential oil and its major compound, 1, 8-cineole, from Eucalyptusglobulus Labill., against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare. J. Stored Prod. Res. 45, 108–111.
Ward, J.H., 1963. Hierarchical grouping to optimize an objective function. J. Am. Stat. Assoc. 58, 66–103.
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SS contributed in collecting plant sample, running the laboratory work. TSF drafted the paper and contributed to critical reading of the manuscript. HDF contributed in collecting plant sample and identification, confection of herbarium and morphological description of the species. LLB contributed to the statistical analyzes. MHRcontributed in collecting plant sample and identification. LMFT contributed to critical reading of the manuscript. PHF contributed to chromatographic analysis. JRP designed the study, supervised the laboratory work contributed to biological and chemical studies,chromatographic analysis and critical reading of the manuscript. All the authors had read the final manuscript and approved the submission contributed to biological studies running the laboratory work, analysis of the data
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de Sá, S., Fiuza, T.S., Borges, L.L. et al. Chemical composition and seasonal variability of the essential oils of leaves and morphological analysis of Hyptis carpinifolia. Rev. Bras. Farmacogn. 26, 688–693 (2016). https://doi.org/10.1016/j.bjp.2016.05.011
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DOI: https://doi.org/10.1016/j.bjp.2016.05.011