Abstract
A methanol extract from the whole plant of Dendrobium formosum Roxb. ex Lindl., Orchidaceae, showed inhibitory potential against α-glucosidase and pancreatic lipase enzymes. Chromatographic separation of the extract resulted in the isolation of twelve phenolic compounds. The structures of these compounds were determined through analysis of NMR and HR-ESI-MS data. All of the isolates were evaluated for their α-glucosidase and pancreatic lipase inhibitory activities, as well as glucose uptake stimulatory effect. Among the isolates, 5-methoxy-7-hydroxy-9,10-dihydro-1,4-phenanthrenequinone (12) showed the highest α-glucosidase and pancreatic lipase inhibitory effects with an IC50 values of 126.88 ± 0.66 μM and 69.45 ± 10.14 μM, respectively. An enzyme kinetics study was conducted by the Lineweaver-Burk plot method. The kinetics studies revealed that compound 12 was a non-competitive inhibitor of α-glucosidase and pancreatic lipase enzymes. Moreover, lusianthridin at 1 and 10 μg/ml and moscatilin at 100 μg/ml showed glucose uptake stimulatory effect without toxicity on L6 myotubes. This study is the first report on the phytochemical constituents and anti-diabetic and anti-obesity activities of D. formosum.
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PI contributed in isolation and purification of the compounds and running the laboratory work. NC and CS contribution included the analysis of the data and drafted the paper. TK and WP contributed in cell-based assay of glucose uptake and cytotoxicity. KL and BS contributed in supervision of the laboratory work and critical reading of the manuscript. All the authors have read the final manuscript and approved the submission.
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Inthongkaew, P., Chatsumpun, N., Supasuteekul, C. et al. α-Glucosidase and pancreatic lipase inhibitory activities and glucose uptake stimulatory effect of phenolic compounds from Dendrobium formosum. Rev. Bras. Farmacogn. 27, 480–487 (2017). https://doi.org/10.1016/j.bjp.2017.05.005
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DOI: https://doi.org/10.1016/j.bjp.2017.05.005