Abstract
Along with the development of new materials, advanced medical imaging and surgical techniques, osseointegrated dental implants are considered a successful and constantly evolving treatment modality for the replacement of missing teeth in patients with complete or partial edentulism. The importance of restoring the peripheral neural feedback pathway and thus repairing the lack of periodontal mechanoreceptors after tooth extraction has been highlighted in the literature. Nevertheless, regenerating the nerve fibers and reconstructing the neural feedback pathways around osseointegrated implants remain a challenge. Recent studies have provided evidence that platelet-rich plasma (PRP) therapy is a promising treatment for musculoskeletal injuries. Because of its high biological safety, convenience and usability, PRP therapy has gradually gained popularity in the clinical field. Although much remains to be learned, the growth factors from PRP might play key roles in peripheral nerve repair mechanisms. This review presents known growth factors contributing to the biological efficacy of PRP and illustrates basic and (pre-)clinical evidence regarding the use of PRP and its relevant products in peripheral nerve regeneration. In addition, the potential of local application of PRP for structural and functional recovery of injured peripheral nerves around dental implants is discussed.
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Introduction
As one of the major advances in dental medicine in the past 50 years, dental implants have revolutionized the traditional way of oral rehabilitation. By reestablishing physiological function and even promoting general health, treating complete or partial edentulous patients using dental implants is regarded as an ideal treatment plan today.1 In addition to these generally known benefits of replacing lost teeth with dental implants, the natural periodontal ligament and the intimate contact between collagen fibers and Ruffini mechanoreceptive terminals (e.g., myelinated and unmyelinated nerve fibers) are thought to be inevitably impaired by tooth loss.2 However, this latter assumption is contradicted by a phenomenon, first described by Professor P-I Brånemark, in which lower-limb amputees walking while wearing bone-anchored prostheses are able to differentiate between different soils.3, 4, 5 Furthermore, patients with oral implants have reported a gradual increase in their tactile function over time.6
This phenomenon, known in the literature as “osseoperception”, originates from the bone in the immediate vicinity of the inserted implants.4 In general terms, this tactile perception has been defined as the mechanosensibility associated with osseointegrated implant rehabilitation, which can be transduced by various kinds of mechanoreceptors located in the oro-facial region, such as in the muscle, joints, oral mucosa, periosteal tissues and skin.7 An animal study from our group demonstrated that myelinated nerve fibers densely populated the peri-implant mucosa and apical regions and, to a lesser extent, were also detected in the woven bone and osteons near the implant threads,8 indicating that the peri-implant soft tissue can play a role in the repair and regeneration of sensory nerve endings. Recent reports even show that an osseointegrated implant can at least partly restore the peripheral neural feedback pathway.9, 10
Clinical evidence in favor of the osseoperception concept includes signs of the existence of regenerated nerve fibers around implant threads,11 recovery of tactile function12 and even activation of the primary sensorimotor cortex while dental implants are mechanically stimulated.13 These findings add to the evidence that peri-implant nerve tissue possesses the inherent capacity to regenerate after tooth extraction, albeit in most cases on a quite basic level.
In an attempt to further enhance osseoperception, novel treatment strategies have been proposed involving reconstruction of the peri-implant ligament,14 transplantation of Schwann cells (SCs),15 injection of neuropeptides (e.g., calcitonin gene-related peptide-α)16 and various implant placement and loading protocols.8 However, none of the aforementioned principles have reached true proof-of-principle status, and their potential clinical use remains questionable to date.
On the basis of the currently available evidence, platelet-rich plasma (PRP), which contains growth factors that positively influence neural cell survival, might have the potential to promote peripheral nerve regeneration.17, 18 Thus, the following review summarizes the state-of-the-art application of PRP for recovery of injured peripheral nerves and offers an outlook on possible future use as an emerging strategy for osseoperception. The articles cited in the review, published up to the 15 November 2016 and including electronic early-release publications, were acquired using three databases: MEDLINE (PubMed), Web of Science (ISI Web of Knowledge) and OpenGrey (System for Information on Grey Literature in Europe).
PRP treatment: preparation and biological characteristics
As a promising autogenous graft, PRP has been a focus in regenerative medicine in recent years. Following the publications by Whitman et al.19 and Marx et al.,20 the application of PRP has gained increasing popularity in the medical field, especially in oral and maxillofacial surgery. In the past decade, PRP has been developed via a variety of commercial and home-made techniques, and thus various forms of PRP exist, including plasma rich in growth factors (PRGFs),21 cell separator PRP,22 Choukroun’s platelet-rich fibrin,23 concentrated PRP,24 platelet-leukocyte gel25 and simplified PRP products called concentrated growth factors.26 In daily practice, most clinicians apply one or two specific PRP products according to their own experience, in liquid or solid form.
To avoid confusion in the interpretation of published results, there is a clear need to accurately describe the products that are tested and to try to classify them accordingly (Table 1).27, 28, 29 Ehrenfest et al. suggested that the various platelet concentrates can be classified into four main categories, depending on the known leukocyte content and fibrin architecture:30, 31, 32 pure platelet-rich plasma (P-PRP), leukocyte- and platelet-rich plasma (L-PRP), pure platelet-rich fibrin (P-PRF), and leukocyte- and platelet-rich fibrin (L-PRF). Given this variety of preparations and applications of platelet concentrates,32 studies do not always adhere to the proposed classification, which leads to inconsistent results and confusion in comparing the efficacy of PRP products. Thus, the term “PRP” is used in the present review to include all known terms, and specific names will be used only when applicable.
In general, PRP is derived from the centrifugation of autologous whole blood and finally forms a gel-like structure when mixed with thrombin and calcium chloride.33 After the activation of platelets, a cocktail of growth factors with mitogenic and chemotactic characteristics are induced and released from the platelets’ α-granules, an organelle critical for wound healing. Instead of the effect being limited by the use of a single growth factor, the multiple factors together mimic the complex situation of natural tissue repair and improve processes such as angiogenesis, inflammation and the immune response. There is also convincing evidence that the survival of sensory neurons depends on multiple neurotrophic factors acting synergistically or in a defined sequence.34 The antimicrobial potential of PRP, owing to the leukocytes and neutrophils it contains, was also reported in a recent systematic review of available preclinical evidence and is one of PRP’s critical characteristics in controlling postoperative infections at surgical sites.35
A further advantage is that all growth factors, proteins and cytokines from PRP are autologous and non-toxic. This treatment using the patient’s own blood could avert complex ethical issues, immunological rejections or similar adverse effects known to exist with allograft or xenograft materials. Compared to an artificially synthesized single growth factor or most of the other biomaterials available on the market, the clinical collection and preparation of PRP are convenient, efficient and economic.36 In addition, the gel-like consistency of PRP is beneficial for its application into small bone defects, and it is not easily washed away from the surgical site.
The role of growth factors from PRP on nerve regeneration
A growing body of studies has demonstrated that topical PRP application is effective in soft-tissue healing and bone regeneration in implant surgery.37, 38, 39 The potential of PRP in modulating soft- and hard-tissue formation and regeneration depends on the levels of released growth factors.40 For peripheral nerve regeneration, as summarized in Table 2,41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52 various known cytokines and growth factors within platelet-rich plasma, including transforming growth factor-β (TGF-β), platelet-derived growth factor, vascular endothelial growth factor, insulin-like growth factor and basic fibroblast growth factor (bFGF), have been discussed as being crucial for cell proliferation and differentiation, stimulation of angiogenesis and scar control in the process of regenerating peripheral nerve structures. Although the mechanism by which these growth factors work together and interact efficiently for wound healing remains incompletely understood,53 preliminary studies have already demonstrated positive effects from PRP acting as a pool of growth factors.
Evidence from clinical studies
When traumatic gaps of nerve structures are <3 cm long, PRP has shown the ability to induce nerve regeneration.17 To regenerate axons across nerve gaps longer than 3 cm, it is suggested that PRP be applied with a bridging graft material that both supports and promotes axon regeneration. Two case reports have suggested that a fibrin clot could help with full functional recovery after surgical repair of a totally transected abducens nerve.54 PRF together with a collagen tube has been shown to induce appropriate sensory and motor recovery across a 12-cm-long ulnar nerve gap.55 A case report demonstrated PGRF’s therapeutic potential for the treatment of common perineal nerve palsy associated with multiple ligament injuries of the knee.56 A report of a case in which PRGF was used for the treatment of bisphosphonate-related osteonecrosis of the jaw showed recovered sensitivity and partially regenerated bone in a 1-year follow-up.57 The fact that defects around dental implants are normally not as large as peripheral nerve defects might make the regeneration of peri-implant nerve fibers more feasible because of the reduced need for added biomaterials; PRP can be used alone.
Investigations in the field of functional neural recovery have reported that PRP prompts the re-establishment of sensitivity within 2 weeks of observation for patients with peripheral neuropathy induced by leprosy58 and improves upper-limb function by 3-month follow-up.59 Similarly, a preliminary clinical study found that, when a modified inferior alveolar nerve lateralization technique was performed, the covering of the inferior alveolar nerve with platelet-rich materials and a collagen membrane could result in accelerated recovery of sensation within the first 6 months as evaluated by subjective two-point discrimination and static light touch.60 Furthermore, this treatment method could significantly reduce the duration of the initial discomfort. A recent split-mouth designed human study61 indicated that the use of L-PRF as a socket-filling material could achieve not only the preservation of horizontal and vertical ridge dimension but also the regrowth of blood vessels and nerve axons (Figure 1) in the anterior maxillary regions at 3 months after tooth extraction.
However, a randomized, controlled and single-blinded clinical study found that PRP drops alone had no positive effect on the recovery of corneal sensitivity after laser therapy, although beneficial effects were observed in the promotion of epithelial healing.62 This finding is probably due to the limited bioavailability of growth factors on the intact corneal stroma when the substance is topically administered.
It is also worth mentioning that, for ethical reasons, histological analyses of human samples to investigate neural regeneration are rare. In most cases, functional and morphologic evaluations, such as ankle stance angle, walking track analysis and electrophysiologic tests, are not only the recommended methods but also the few feasible options for studies of peripheral nerve regeneration.63 For clinical situations in implant dentistry, oral psychophysical studies and magnetic resonance imaging tests5 are usually performed to evaluate and monitor the osseoperception around implants.
Evidence from basic research
Although relatively few human trials have involved PRP therapy, there is mounting evidence from in vitro and animal experiments. PRP has been used to study the effect of stimulating neural regeneration of the rat facial nerve,64 rat sciatic nerve,65, 66, 67, 68, 69, 70, 71, 72, 73, 74 rat cavernous nerve,75, 76 guinea pig facial nerve,77 rabbit sciatic nerve,78 dog anterior cruciate ligament79 and rat spinal cord.80 These studies presented positive results regarding functional nerve recovery,64, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 80 regeneration of axons,64, 65, 70, 71, 72, 75, 76 g-ratio (an index of optimal axonal myelination),67 thickness of the myelin sheath,66, 70, 77, 78 nerve conduction velocity,78 revascularization and reinnervation,79 and axon diameter,69, 70, 80 with observation periods ranging from 2 to 12 weeks postoperatively.
In addition, two recent studies have suggested that concentrated growth factors (CGFs) could promote not only the migration of SCs in vitro81 but also functional recovery in a rat model of sciatic nerve crush injury.18 By contrast, one study82 reported that platelet gel with a collagen tube conduit did not have a beneficial effect on rat sciatic nerve regeneration 12 weeks after microsurgical reconstruction. However, 2 years later, the same group83 observed that the same gel could have a positive effect on nerve repair. The discrepancy between the studies may be due to differences in histological processing or to excessive stimulation of collagen formation in the initial study by growth factors from the platelet gel, including TGF-β and bFGF.
Although numerous relevant animal studies have been carried out, only a few of these investigations clearly provided specific platelet concentrations.67, 68, 72, 84, 85, 86 In rat studies, the average whole-blood platelet count has been reported to be 478.12 × 103 μL−1, and the average platelet count of PRP was 3 221.34 × 103 μL−1,72 whereas the effective concentration of platelets in another investigation using rats was 1 500 × 103 platelets per μL.67 Similarly, in an ovine model, restoration of motor function at 12 weeks after peroneal nerve compression injury was achieved using scaffolds and PRGF injections; the platelet count was 202 × 103–349 × 103 μL−1.86 Considering the relatively wide range of platelet counts in these studies, it would be of importance for future studies to evaluate in more detail the effective range of platelet concentration, whole-blood platelet counts and, if applicable, their growth factor levels.
Furthermore, an in vitro study indicated that the appropriate concentration of PRP from rats, with an average whole-blood platelet count of 536 × 103 μL−1 and a PRP platelet count of 3 481.75 × 103 μL−1, has the potential to stimulate proliferation and migration of SCs, which are regarded as the primary structural and functional cells of the peripheral nervous system.85 An in vitro investigation noted that the addition of PRP, with a mean whole-blood platelet count of 186.35 × 103 μL−1 and a PRP platelet count of 1 237.28 × 103 μL−1, could facilitate the potential use of human adipose-derived stem cells in nerve regeneration.84
Thus far, most of the in vivo and in vitro results mentioned above have supported promising future prospects for local application of PRP, CGF or PRGF in functional axon recovery; however, the efficacy of PRF in the regeneration of peripheral nerves is still questionable, and the related studies are far fewer than those using PRP. As reported by an in vivo study, PRP and PRF were both effective in the functional recovery of a 10-mm sciatic nerve injury 90 days after surgery, with a mean whole-blood platelet count of 460 × 103 μL−1, a PRP platelet count of 2 623 × 103 μL−1 and a mean PRF platelet count of 35 × 103 μL−1, albeit without a significant difference between axonal diameter and myelin thickness.68 In contrast, PRF failed to increase functional recovery in a 5-mm sciatic nerve gap as shown by functional, histopathologic and electrophysiologic analyses.87 Although the PRF in these two studies was produced using the same protocol described by Choukroun et al.,23 the effective concentrations of activated platelets and released growth factors were not examined in the latter study, making it almost impossible to know whether the observed differences were due to inter-individual variation or the preparation method.
Hypothesis: peripheral nerve regeneration after extraction using PRP and immediate implant placement
After the extraction of teeth, mechanoreceptors in the periodontal ligament are subject to immediate degeneration. A novel idea to stimulate peripheral nerve regeneration after tooth loss would be to add a multitude of growth factors from autologous PRP together with an implant placement immediately after tooth extraction to overcome degenerative neural processes. Gene transcription, neural progenitor cells and nerve remodeling are dynamically regulated and induced in the regeneration cycle and in response to growth factors and respective signaling pathways, such as phosphatidylinositol-3 kinase (PI3K)-Akt and Ras/extracellular signal-regulated kinase, that are required for neurite survival and outgrowth.88 The whole process could not only directly enhance the regeneration of nerve fibers but also indirectly induce angiogenesis, myelin formation, and SC proliferation and differentiation, and even activate potential stem cell lines in the region of the implants.
Over time, this remodeling process, which functions in a synergistic way, might even increase the metabolic balance of regenerated tissues during the establishment of osseoperception and promote proprioceptive sensory feedback, enabling a well-coordinated masticatory function. This might avoid overload during masticatory function and help maintain the overall health of the temporo-mandibular joint and neuromuscular system in patients treated with a dental implant (for more detail see Figure 2).
Implications for future studies
Most of the research on dental implants has been focused on osseointegration and bone regeneration due to limitations in bone availability in the upper or lower jaw prior to planned implant treatment. Nevertheless, not only does rehabilitation with dental implants aim to acheive primary or secondary implant stability and osseointegration, but a functional implant resulting in well-coordinated masticatory function is also of great importance to ensure long-term success and patient satisfaction. Therefore, the relevance and accomplishment of osseoperception will hopefully receive more attention in research and daily clinical practice in the future. The following paragraphs highlight some areas where more knowledge is urgently needed.
The process of PRP preparation and its therapeutic concentration
Although autologous blood concentrations may offer promising treatment options for peripheral nerve injury in the oral cavity, few studies have focused on the effects of PRP therapy on peri-implant sensory feedback. It is essential to realize that a large number of variables may influence the therapeutic efficacy of PRP treatment, which may partly explain the conflicting results in different experiments.89
One of these important factors is the process of PRP preparation itself, which may result in different concentrations of platelets among patients treated and thus in broad variability of the growth factor ratio.90 Higher concentrations of platelets in PRP may not necessarily result in positive cell proliferation;91 instead, an optimal (not yet defined) concentration range of PRP would help to keep those growth factors active and functioning well for a longer time period. Hence, it is reasonable to assume that an ideal PRP concentration—perhaps even tailor-made for each patient treated—could maximize the therapeutic benefits of nerve regeneration and wound healing.
Other factors contributing to the unpredictability and complexity of the therapeutic efficacy of PRP in peripheral nerve regeneration are differences in the method of activation (calcium chloride or thrombin), mode of application (injection or implantation of a PRP gel), storage temperature (−80 °C frozen or room temperature) and frequency of application.
A potential therapeutic level of platelet concentration for bone and soft-tissue regeneration has been reported, namely, 1 000 × 103 μL−1 (four- to fivefold the baseline platelet numbers).92 However, less is known about the optimized dose–response curve regarding nerve regeneration. Cell culture experiments85 have shown that 2.5%–20% PRP (87.5 × 103–700 × 103 μL−1) from Sprague–Dawley rats could significantly stimulate SC proliferation and migration in a concentration-dependent manner compared to untreated controls. However, when higher PRP concentrations (40%, 1 400 × 103 μL−1) are used, the above-mentioned effects on SCs are suppressed. The present study suggests that there may exist different types of dose responses, reaction rates or complicated interactions between nerve and bone regeneration when PRP products are used.
Timing of treatment
It has been suggested that muscle tissue could progressively transform into irreversible fibrosis if it is not reinnervated within 1–2 years.17 Therefore, the timing of the regenerative intervention seems to be another factor influencing the overall treatment strategy,93 after which the target tissue may lose the ability to be reinnervated. However, regarding the immediate and delayed implant placement protocols, there are insufficient data exhibiting obvious differences in the innervation of peri-implant tissues between the two protocols.5 More recently, our group observed in a randomized split-mouth study that immediate implant placement and an immediate loading protocol were more likely to lead to optimized peri-implant reinnervation,8 suggesting that the timing of treatment for peri-implant reinnervation is a crucial issue to consider when using PRP products.
Because of the short life of platelets —approximately 5–7 days—the effect of PRP on bone repair may be active only in the early stage of wound healing.94 As a practical example of the importance of timing when applying PRP in oral surgery, immediately after tooth extraction, in the initial phase of acute axon injury, application of PRP therapy might be valuable for maximizing the neural regeneration and thus for prevention of an excessive load of dental implants. On the other hand, for patients who lost their teeth a long time ago, it might be more effective to combine certain stem cells with PRP therapy in order to maintain continuous availability of the bioactive effects.
Animal models for feasibility experiments
Despite more reliable and accurate results from human clinical trials, for ethical reasons, preclinical experiments are indispensable to the study of PRP therapy for peripheral nerve injury and regeneration. Appropriate animal models, chosen according to various objectives, are likely to produce convincing and clinically relevant results. Thus, it is important to use an animal model with characteristics comparable to those of the ultimate treatment cohort,95, 96 including common aspects of anatomy, physiology, histology, pathology and even gene expression. For research in implant dentistry, the animal model should have relatively large teeth and extraction sockets that are convenient for surgery, and it should be possible to take radiographs at the surgical site during follow-up. It is also desirable for the animal model to be inexpensive, readily available and easy to anesthetize.
A small variety of animal models, including dogs, mini-pigs and non-human primates, are frequently used in analyzing bone-implant interactions owing to their similarity to human bone physiology and pathology.97, 98 Although non-human primates offer the most similarity to human bone with respect to anatomy and physiology, mini-pigs are more widely used because of less critical ethical issues and low purchase and maintenance costs, in addition to easy handling. Unlike pigs, dogs (the “canine model”) with stand repetitive blood sampling during PRP preparation, and their mandibles are large enough to receive commercial implants. Furthermore, the characteristics of human bone have been reported to be better approximated by the properties of dog bone in comparison to that of pigs, cows, sheep, chicken and rats.99 Therefore, the canine model should be regarded as the preferred animal model in experimental research using PRP.
Conclusions
Taken together, current evidence from in vitro and animal experiments and preliminary clinical trials has supported the possibility that peri-implant sensory feedback can be modified and promoted by various growth factors released from autologous PRP. Additional studies are needed to prove the hypothesis that combining PRP with dental implants is an effective and safe treatment concept to convey peripheral neural signals to the central nervous system in a functional manner and thus improve osseoperception. To maximize the regeneration of injured peripheral nerves around dental implants, several points should be considered in future studies:
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An optimized preparation of PRP is essential for the biological activity and clinical efficacy of growth factors within PRP. All variables of PRP use, such as the concentration of platelets and growth factors, method of activation, mode of application and frequency of application, should be carefully explored and reported.
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The exact roles and interactions of the growth factors in PRP remain unclear, which is also the case for newer products based on PRP, such as PRF and CGF.
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The appropriate timing of intervention is of great importance for optimal nerve regeneration. To maximize the structural and functional recovery of injured nerves in combination with PRP, it might be preferable to insert dental implants immediately after tooth extraction following nerve injury.
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Considering the advantages and limitations of different animal models, the canine model should be regarded as the preferred animal in preclinical research of PRP treatment.
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Although not easy to accomplish from a practical perspective, prospective clinical studies with a randomized controlled design would be ideal for analyzing the potential risks and benefits of PRP in promoting osseoperception.
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Acknowledgements
We thank Mrs Bernadette Rawyler, medical illustrator, School of Dental Medicine, University of Bern, for the preparation of Figure 2. Fellowship support was received from Research Foundation Flanders (FWO) from the Belgian government and the Sichuan Province Science and Technology Support Program (2016SZ0010).
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Huang, Y., Bornstein, M., Lambrichts, I. et al. Platelet-rich plasma for regeneration of neural feedback pathways around dental implants: a concise review and outlook on future possibilities. Int J Oral Sci 9, 1–9 (2017). https://doi.org/10.1038/ijos.2017.1
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DOI: https://doi.org/10.1038/ijos.2017.1
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