Abstract
PI3Kδ plays pivotal roles in the maintenance, proliferation and survival of malignant B-lymphocytes. Although not curative, PI3Kδ inhibitors (PI3Kδi) demonstrate impressive clinical efficacy and, alongside other signaling inhibitors, are revolutionizing the treatment of hematological malignancies. However, only limited in vivo data are available regarding their mechanism of action. With the rising number of novel treatments, the challenge is to identify combinations that deliver curative regimes. A deeper understanding of the molecular mechanism is required to guide these selections. Currently, immunomodulation, inhibition of B-cell receptor signaling, chemokine/cytokine signaling and apoptosis represent potential therapeutic mechanisms for PI3Kδi. Here we characterize the molecular mechanisms responsible for PI3Kδi-induced apoptosis in an in vivo model of chronic lymphocytic leukemia (CLL). In vitro, PI3Kδi-induced substantive apoptosis and disrupted microenvironment-derived signaling in murine (Eμ-Tcl1) and human (CLL) leukemia cells. Furthermore, PI3Kδi imparted significant therapeutic responses in Eμ-Tcl1-bearing animals and enhanced anti-CD20 monoclonal antibody therapy. Responses correlated with upregulation of the pro-apoptotic BH3-only protein Bim. Accordingly, Bim−/− Eμ-Tcl1 Tg leukemias demonstrated resistance to PI3Kδi-induced apoptosis were refractory to PI3Kδi in vivo and failed to display combination efficacy with anti-CD20 monoclonal antibody therapy. Therefore, Bim-dependent apoptosis represents a key in vivo therapeutic mechanism for PI3Kδi, both alone and in combination therapy regimes.
Similar content being viewed by others
Introduction
Secondary lymphoid organs (SLOs) provide a key survival niche for neoplastic B-cells. Here cells receive a milieu of pro-survival signals, including those emanating from the B-cell receptor (BCR), chemokine/cytokine receptors, integrins and specific cell–cell interactions. Collectively, these pathways contribute toward malignant cell proliferation, survival and therapeutic resistance.1
Since phosphatidylinositol-3 kinase (PI3K) signaling is vital in many of these processes, its inhibition represents an attractive therapeutic strategy. Class I PI3Ks represent a prime target in hematological malignancies due to their roles in linking cell surface receptors to downstream kinase activation in lymphocytes (for example Akt and Btk).2, 3 PI3Ks are heterodimeric, comprising a p110 catalytic subunit and a p85 regulatory subunit. Mammalian systems exhibit multiple isoforms of class I PI3K catalytic subunits (namely, p110α, p110β, p110δ and p110γ), which display tissue-specific expression patterns and non-redundant roles in development.4 Both p110α and p110β are expressed ubiquitously,5, 6 whereas p110δ and p110γ are largely leukocyte restricted.4
Accordingly, mice deficient in p110δ (referred to as PI3Kδ henceforth) activity exhibit profound disruption of lymphocyte homeostasis and humoral immunity7 via effects centered upon antigen receptor signaling,7, 8 cytokine production8, 9 and Treg function.10 Consequently, δ isoform selective PI3K inhibitors (PI3Kδi) have provided encouraging therapeutic responses in clinical trials,11 particularly in combination with anti-CD20 monoclonal antibodies (mAb),12 culminating in the approval of Zydelig (idelalisib) for the treatment of relapsed refractory CLL in combination with rituximab. Although the therapeutic potential of these agents is unquestionable, the exact in vivo therapeutic mechanism remains ambiguous. With the ever-increasing number of novel therapeutic agents, the challenge is to identify the most efficacious, potentially curative, drug combinations. A clear mechanistic understanding of how these agents work will help provide a rational framework for improved efficacy and the circumvention of resistance mechanisms, which have emerged for other small molecule inhibitors.13
Potential PI3Kδi in vivo effector mechanisms can be stratified into those influencing the malignant cell directly (intrinsic) and those mediating effects on the host immune system (immunomodulatory effects). The latter occurs through Treg suppression, resulting in enhanced anti-tumor immunity in solid tumor models.10 In contrast to solid tumors, PI3Kδ is often expressed within malignant lymphocytes themselves; therefore, additional malignant cell intrinsic mechanisms are likely to exist in hematological cancers. These include inhibition/alteration of tissue homing,14 microenvironment-derived support15, 16 and BCR-mediated survival signals.14
It is likely that these effects are integrated in vivo and collectively modulate malignant cell survival through regulation of intrinsic apoptosis.17, 18 Intrinsic apoptosis is regulated by members of the Bcl-2 family. Under normal conditions, the pro-apoptotic activities of activated Bax/Bak are repressed via association with pro-survival Bcl-2 family members (Bcl-2, Bcl-XL, Bcl-w, Mcl-1 and Bfl-1/A1).19 Following apoptotic stimuli, pro-survival molecules are inhibited by association with pro-apoptotic BH3-only proteins (Bad, Bid, Bik, Bim, Bmf, Hrk, Noxa and Puma) and Bax/Bak subjected to further activation by a subset of these proteins.20, 21 Subsequently, cell death ensues following saturation of pro-survival molecules and de-repression of activated Bax/Bak.21
Bim is a major regulator of immune homeostasis, since Bim−/− animals exhibit expanded lymphocyte populations and increased autoreactivity.22, 23 In B-cells, this homeostatic control manifests through BCR-mediated upregulation of Bim expression during immature B-cell negative selection,22 although additional BH3-only proteins also contribute.24 Furthermore, BCR signals maintain mature B-cell populations via a PI3K-dependent mechanism, which involves suppression of Bim.25 Similarly, soluble factors CXCL12, BAFF and APRIL elicit their pro-survival effects either through suppression of Bim26, 27, 28 or increased expression of pro-survival Bcl-2 family members.29
On the basis of the key role of PI3Kδ in these processes, we hypothesized that PI3Kδi disrupt multiple pro-survival inputs culminating in Bim-mediated intrinsic apoptosis and in vivo clearance of malignant cells. Although prior studies have been performed assessing PI3Kδi-mediated immunomodulation, only limited data are available assessing the impact of PI3Kδ inhibition within a malignant target in vivo. Using primary human and mouse (Eμ-Tcl1 Tg) CLL cells alongside a highly selective PI3Kδi, GS-9820,30 we demonstrate that intrinsic apoptosis driven by Bim is the central in vivo therapeutic mechanism for PI3Kδi. This knowledge allowed the rational design of a complementary drug combination strategy incorporating inhibitors of PI3Kδ and Bcl-2 (Venetoclax). This approach proved highly efficacious in vivo, effectively halting leukemia progression in treated animals.
Materials and methods
Patients and cells
Diagnosis of CLL was according to the IWCLL-NCI 2008 criteria.31 Samples were studied following ethical committee approval (REC reference: 228/02/t) under the Declaration of Helsinki. Malignant cell isolation, determination of purity, and in vitro culturing were described previously.32 Eμ-Tcl1 Tg leukemias were isolated from splenocytes by density gradient centrifugation and maintained in RPMI-1640 supplemented with 10% fetal calf serum, 1 mM pyruvate, 2 mM glutamine, 45 units/ml penicillin, 45 μg/ml streptomycin (Thermo Fisher, Loughborough, UK), 50 μM 2-mercaptoethanol and 200 μM L-asparagine (Sigma, Gillingham, UK). Samples exhibiting >85% CD5+B220+ cells were used directly. Samples with <85% CD5+B220+ cells were first purified using a Mouse Pan B-Cell Isolation kit (Miltenyi Biotec, Bisley, UK).
Animals
Animals were maintained in local facilities and experiments approved by local ethical committees under Home Office license PPL30/2964. Eμ-Tcl1 Tg mice33 were a gift from Dr Egle (Salzburg Cancer Research Institute, Salzburg, Austria) following permission from Dr Pekarsky and Professor Croce (Ohio State University, Ohio, USA). Eμ-Tcl1 Tg animals were crossed with Bim−/− animals (Jackson Laboratory, Bar Harbor, ME, USA) or Vav-Bcl-2 Tg animals (obtained from Professor Hacker following permission from Professor Adams). Animals were monitored for disease presentation by monthly blood sampling and CD5 × B220/CD19 flow cytometry. Animals were killed once 2 out of 3 criteria were met: (1) CD5+ B-cells >80% of circulating lymphocytes, (2) spleen (determined by palpation) >30 mm and (3) total white blood cell (WBC) counts >5 × 107/ml. In transplant experiments sex-matched severe combined immunodeficiency syndrome (SCID) mice (Charles River, Saint-Germain-Nuelles, France) were inoculated with 1 × 107 Eμ-Tcl1 Tg splenocytes and monitored for disease presentation. Animals were killed when 2 out of 3 criteria were met: (1) spleen >30 mm, (2) WBC counts >5 × 107/ml and (3) illness requiring euthanasia.
Antibodies and inhibitors
Anti-mouse CD20 (Clone: 18B12) mouse IgG2a was produced in-house. 18B12 was administered in vivo by intraperitoneal injection of 250 μg in phosphate-buffered saline. Anti-CD20-mediated opsonization of human targets was performed using Rituximab, which was gifted by Southampton General Hospital oncology pharmacy. GS-9820 was provided by Gilead Sciences Inc., (Foster City, CA, USA) and administered in vivo at 10 mg/kg formulated in 0.5% methylcellulose 0.1% Tween-80 per os, bis in die (BID). ABT-199 was administered in vivo per os by formulation in 60% Phosal-50 PG (Lipoid, Ludwigshafen, Germany), 30% PEG-400, 10% ethanol at 50 mg/kg for 7 days followed by 100 mg/kg for a further 14 days. Further inhibitors and antibodies utilized are detailed in the Supplementary methods.
Cellular assays
Annexin V/propidium iodide (PI) assays were performed as previously described.24 For further details and other methods see Supplementary methods.
Statistics
Data were expressed as mean±s.e.m. Statistical significance was assessed by Student’s t-test or two-way ANOVA. Survival was assessed via Mantel–Cox statistical analysis.
Results
PI3Kδ inhibition disrupts BCR-induced signaling and adhesion in Eμ-Tcl1 Tg leukemias
To investigate the in vivo therapeutic mechanism of PI3Kδi, we utilized the Eμ-Tcl1 Tg model, a gold standard for aggressive CLL.33, 34 Consistent with previous reports35, 36 our cohort presented with a CD5+ B-cell leukemia with phenotypic hallmarks of CLL, extensive splenomegaly and a median survival of 294 days (Supplementary Figures 1–2). To inhibit PI3Kδ, we utilized the PI3Kδi GS-9820 (Supplementary Figure S3), recently the subject of clinical trials,37 as it has excellent isoform specificity30 and better pharmacokinetic properties than idelalisib in the mouse (Gilead unpublished observations and Supplementary Figure 3).
BCR stimulation increased phosphorylation of the PI3K-target Akt at both S473 and T308, which was inhibited by prior application of GS-9820 in a dose-dependent manner (Figure 1a and Supplementary Figure 4a). Concurrent inhibition of ERK, but not Syk, phosphorylation was also evident (Supplementary Figure 4b). GS-9820 also significantly inhibited BCR-induced adhesion to fibronectin (Figure 1b), yet in contrast to inhibitors of Btk (ibrutinib) and Syk (R406) had no impact on BCR internalization (Figure 1c). These data indicate that GS-9820 effectively inhibits BCR-induced kinase signaling and impairs some functional consequences of BCR stimulation.
PI3Kδ inhibition impairs chemotaxis and pro-survival responses to microenvironment-derived factors in Eμ-Tcl1 Tg leukemias
To assess the impact of GS-9820 on cellular migration and tissue homing responses, Eμ-Tcl1 Tg leukemias were examined for surface expression of chemokine receptors. Eμ-Tcl1 Tg leukemias exhibited heterogeneous surface staining for CXCR4, CXCR5 and CCR7, but were negative for CXCR3 (Figure 2a). Although CXCR4 and CXCR5 expression was similar to that of normal B-cells, CCR7 was significantly reduced. Accordingly, both CXCL12 and CCL21 induced transmigration of Eμ-Tcl1 Tg leukemias in transwell assays (Figure 2b). GS-9820, R406 and ibrutinib inhibited, but did not ablate, CXCL12-mediated chemotaxis, as previously identified (Figure 2b).14, 38, 39 Consistent with this, GS-9820 inhibited CXCL12-induced phosphorylation of AktS473 (Figure 2c), yet in contrast to BCR signaling was unable to concomitantly inhibit ERK phosphorylation (Supplementary Figure 4c). These data suggest that CXCL12-mediated chemotaxis and downstream kinase activation is only partially PI3Kδ dependent in Eμ-Tcl1 Tg leukemias.
Since many stroma-derived soluble factors and cellular interactions provide support signals to malignant B-cells, we examined the ability of GS-9820 to impair these processes. CXCL12, BAFF, APRIL or a combination of all three significantly improved the viability of Eμ-Tcl1 Tg leukemias 5 days post ex vivo culture in comparison to medium alone and were effectively antagonized by GS-9820 (Figure 3a). GS-9820 application also inhibited support arising from the follicular dendritic cell line HK. For both CLL and Eμ-Tcl1 cells co-culture with HK cells enhanced viability (Figure 3b, left and Supplementary Figure 4d). This survival advantage was disrupted by addition of GS-9820 after the formation of cell–cell contacts in these co-culture settings (Figure 3b, left and Supplementary Figure 4d). In addition, both GS-9820 and ibrutinib inhibited cell–cell contact formation and adhesion when added before co-culture (Figure 3b, right). These data imply that both cytokine/chemokine and cell–cell contact-mediated support mechanisms are PI3Kδ dependent in this system.
PI3Kδ inhibition reduces cellular viability coincident with upregulation of Bim
Previously, PI3Kδi have been reported to reduce the in vitro viability of malignant B-cells, presumably through BCR inhibition.18 GS-9820 reduced cellular viability in both Eμ-Tcl1 Tg leukemias and CLL samples (Figure 4a); albeit with significant heterogeneity in individual Eμ-Tcl1 Tg tumors. This reduction in viability was coincident with upregulation of Bim expression and Bim–Bcl-2 complex formation, indicative of enhanced Bim function (Figures 4b and c). Similar effects were also observed in some, but not all, CLL samples and diffuse large B-cell lymphoma cell lines (Supplementary Figures 5 and 7).
High pro-survival occupancy with BH3-only proteins sensitizes cells to subsequent apoptotic stimuli due to a reduced capacity to neutralize activated Bax/Bak, known as mitochondrial priming.40 Because of enhanced mitochondrial priming through Bim upregulation, we hypothesized that GS-9820-treated cells would exhibit enhanced sensitivity toward the Bcl-2 inhibitor ABT-199. Indeed, administration of GS-9820 24 h before ABT-199 (to allow Bim upregulation) significantly enhanced cytotoxicity in comparison to ABT-199 treatment alone in Eμ-Tcl1 Tg leukemias, CLL and GS-9820-sensitive diffuse large B-cell lymphoma cell lines (Figure 4c and Supplementary Figures 5 and 7).
In comparison to normal B-cells, Eμ-Tcl1 Tg leukemias exhibited extensive Bcl-2 and Bcl-XL expression, with little or no expression of Mcl-1, Bfl-1/A1 or Bcl-w (Supplementary Figure 8). Comparable levels of Bad and Bid were seen in Eμ-Tcl1 Tg leukemias and normal B-cells. Puma was absent in both, only upregulated following treatment with etoposide and concurrent with p53 induction, demonstrating the integrity of the TP53 pathway in Eμ-Tcl1 Tg leukemias (Supplementary Figure 8c).41 However, significant heterogeneity in basal Bim expression was evident in Eμ-Tcl1 Tg leukemias in comparison to normal B-cells, with the level predictive of overall sensitivity to GS-9820-induced death (Supplementary Figures 8a and b). These data indicate a direct link between PI3Kδ (and its inhibition) and the regulation of Bim.
Genetic loss of Bim does not alter Tcl1-induced leukemiagenesis
Previously, Bim has been identified as a key tumor suppressor in both murine and human cancers, with reduced expression associated with therapeutic resistance.42, 43, 44 To further assess the role of Bim in Tcl1-mediated leukemiagenesis and PI3Kδi therapy, Eμ-Tcl1 Tg animals were crossed with Bim−/− animals. Bim−/− Eμ-Tcl1 Tg animals exhibited an equivalent median survival to Bim+/+ Eμ-Tcl1 Tg albeit with a reduced leukemic burden at sacrifice (Figure 5a). Nonetheless, peripheral blood mononuclear cell, splenocyte and lymph node cell numbers were largely comparable (Figure 5b). Western blot revealed that Bim−/− Eμ-Tcl1 Tg leukemias exhibited comparable levels of Bid, Puma, Bcl-2 and Bcl-XL expression to their Bim+/+ counterparts (Supplementary Figure 9a) indicating that compensatory changes in expression of activator BH3-only proteins, or pro-survival molecules, had not occurred and that the tumors did not display stabilizing p53 mutations. Furthermore, leukemias arising in Bim−/− Eμ-Tcl1 Tg animals exhibited an equivalent immunophenotype, to their Bim+/+ Eμ-Tcl1 Tg counterparts (Figure 5c and Supplementary Figure 9b). Overall, these data indicate that Bim does not function as a tumor suppressor in this model.
Bim−/− Eμ-Tcl1 Tg leukemias respond normally to PI3Kδ-dependent stimuli but are resistant toward PI3Kδi-induced cytotoxicity
Before assessing the impact of loss of Bim on GS-9820-induced cytotoxicity, we first assessed the integrity of PI3K-dependent pathways in Bim−/− Eμ-Tcl1 Tg leukemias and their sensitivity to GS-9820. Like their wild-type counterparts, Bim−/− Eμ-Tcl1 Tg leukemias exhibited enhanced AktS473 phosphorylation following BCR stimulation, which was ablated by GS-9820 in a dose-dependent manner (Figure 6a). Furthermore, CXCL12-mediated chemotaxis remained intact and sensitive to GS-9820 inhibition (Figure 6b). In contrast, genetic loss of Bim imparted significant resistance toward GS-9820- and ibrutinib-induced cytotoxicity in Eμ-Tcl1 Tg tumors, whereas responses toward fludarabine were less affected (Figure 6c). Although significantly inhibited, residual levels of GS-9820- and ibrutinib-induced cell death were evident in Bim−/− leukemias. This residual activity was absent in Eμ-Tcl1 Tg leukemias over-expressing Bcl-2 (Supplementary Figure 10) derived from vav-Bcl-2 Tg Eμ-Tcl1 Tg mice. These data demonstrate that Bim plays a major role in the cytotoxic responses induced by GS-9820 and ibrutinib with a minor contribution by other BH3-only proteins.
PI3Kδi elicit anti-leukemic effects in vivo dependent on Bim
To investigate the mechanisms of PI3Kδi in vivo, SCID animals were inoculated with Eμ-Tcl1 Tg leukemias. These mice lack adaptive immune cells and permit the investigation of mechanisms independent of the reported effects on Treg.10
Following detection of CD5+ B-cells in the blood, mice were treated with 10 mg/kg GS-9820 or vehicle control per os BID. Although PI3Kδ-mutant animals exhibit colitis,7 GS-9820-recipient animals did not demonstrate any adverse effects attributable to treatment (Supplementary Figure 11a). GS-9820-treated animals exhibited a 75% reduction in leukemic burden 4 weeks post administration alongside tumor reductions in the peritoneum and spleen (Figures 7a and b). Therapeutic responses to GS-9820 correlated with enhanced Bim expression (≈1.5-fold) in spleen-resident tumor cells in comparison to vehicle-recipient animals (Figure 7a, right). To dissect the role of Bim-dependent apoptosis in PI3Kδi therapy, Bim−/− Eμ-Tcl1 Tg leukemias were transferred into SCID recipients and assessed for responses toward GS-9820. Bim−/− Eμ-Tcl1 Tg leukemia-recipient animals appeared refractory to GS-9820, with little variation in leukemic burden, spleen deposits or splenomegaly evident (Figures 7b and c).
In addition to Treg inhibition, myeloid-derived suppressor cells (MDSC) have been implicated in PI3Kδi-mediated therapeutic responses.10 In both Bim+/+ and Bim−/− leukemia-recipient animals, the frequency of splenic MDSC populations (CD11b+ Ly6CHigh Ly6GLow monocytic-MDSC (M-MDSC) or CD11b+ Ly6CLow Ly6GHigh polymorphonuclear-MDSC (PMN-MDSC)) were unaffected by PI3Kδi administration (Supplementary Figure 12). Therefore, the therapeutic effects of PI3Kδi monotherapy appear largely dependent on Bim-dependent intrinsic apoptosis within the malignant lymphocyte.
PI3Kδi enhance anti-CD20 mAb therapy in a Bim-dependent manner
Since PI3Kδi effectively combine with anti-CD20 mAb in the clinic,12 we determined whether this synergistic effect was dependent on Bim-induced apoptosis. Anti-CD20 mAbs induced a robust depletion of leukemic targets in Eμ-Tcl1 Tg-recipient animals that was ablated in mice lacking the FcγR γ chain−/−, required for activatory FcγR expression and activity (Supplementary Figure 11b). In agreement with other models45 these data identify activatory FcγR-mediated processes as the major anti-CD20 mAb effector mechanism in the Eμ-Tcl1 Tg model.
Consequently, the impact of GS-9820 on antibody-dependent cellular phagocytosis was examined. Anti-CD20-mediated antibody-dependent cellular phagocytosis appeared unaffected by GS-9820 utilizing either murine or human cells (Figure 8a, left and center). Furthermore, GS-9820 did not influence maximal anti-CD20 mAb-mediated leukemia depletion 48 h post treatment of either Bim+/+ or Bim−/− leukemia-recipient animals (Figure 8a, right). Collectively, these data indicate that augmentation of antibody-dependent cellular phagocytosis is not responsible for the combination effects of GS-9820 and anti-CD20 mAb.
Both single 250 μg dose of anti-CD20 mAb and GS-9820 monotherapy extended overall survival (P<0.05 and P<0.005, respectively) in Bim+/+ Eμ-Tcl1 Tg-recipient animals. Furthermore, anti-CD20 mAb and GS-9820 combination therapy enhanced the duration of anti-CD20-mediated leukemia depletion and overall survival in comparison to mAb therapy alone (P<0.005) (Figure 8b, left panels). In contrast, the therapeutic effect of GS-9820 was lost in both monotherapy and combination therapy treatment groups in Bim−/− Eμ-Tcl1 Tg-recipient animals, whereas anti-CD20 mAb therapy appeared unaffected and enhanced overall survival in comparison to vehicle controls (P<0.005) (Figure 8b, right panels). These data confirm that Bim-dependent apoptosis represents the primary therapeutic mechanism of GS-9820 in both monotherapy and combination therapy regimes.
This knowledge can be employed in the design of subsequent treatment regimes. As a proof of concept, it was reasoned that combinations of GS-9820 and ABT-199 would be highly efficacious in vivo. Both ABT-199 monotherapy and combinations with GS-9820 appeared well tolerated, with no symptoms of toxicity associated with treatment. Although GS-9820:ABT-199 combination-treated animals maintained weight, a slight, non-significant reduction in the rate of weight gain was evident in comparison to other treatment groups (Supplementary Figure 11c). Although ABT-199 monotherapy was ineffective against Bim+/+ Eμ-Tcl1 Tg leukemias, combinations of GS-9820 and ABT-199 proved more effective than GS-9820 monotherapy alone (Figure 8c). This strategy reduced leukemic burden by 95% in comparison to vehicle controls, effectively sensitizing Eμ-Tcl1 Tg leukemias to ABT-199. These findings facilitate the design of novel combination therapies and potentially provide a strategy to overcome microenvironment-derived ABT-199 resistance.46
Discussion
Extensive pre-clinical and clinical studies culminated in the approval of the PI3Kδi Zydelig (idelalisib) for the treatment of relapsed/refractory CLL in combination with rituximab.10, 12, 14 Although efficacious, this combination is not curative and primarily delays disease progression. To permit evidence-based design of potentially curative complementary drug combinations, a detailed mechanistic understanding of PI3Kδ inhibition is required. At present, in vivo mechanistic insights are limited to PI3Kδi-mediated immunomodulation in the treatment of solid tumors (that lack PI3Kδ expression).10 In this setting, PI3Kδi enhance anti-tumor immunity through Treg and MDSC suppression.10 Since malignant lymphocytes often express PI3Kδ4 additional intrinsic mechanisms likely arise in lymphoid cancers. In the present study, an in vivo model of CLL was utilized in immunodeficient recipient animals. Within this system, animals lack adaptive immune cells (including, Treg) and do not exhibit PI3Kδi-dependent augmentation of MDSCs (Supplementary Figure 12), allowing the dissection of intrinsic mechanisms from immunomodulatory effects.
Previous studies suggest that tumor intrinsic mechanisms augment BCR signaling,8, 14 chemokine/cytokine receptor signaling8, 14, 15 and stromal cell support.15, 16 BCR signaling is inextricably linked with regulation of Bim-dependent apoptosis in both its pro-survival and pro-apoptotic signaling modes.22, 24, 25 In mature B-cells, BCR signals maintain B-lymphocyte populations via a PI3K-dependent mechanism linked to downregulation of Bim expression25 and in response to antigen, BCR signaling neutralizes Bim by MEK1-dependent phosphorylation.47 Conversely, in immature B-cells aberrant BCR signaling directly upregulates Bim during negative selection.22, 24 Like BCR signaling, many cytokines/chemokines also modulate cellular survival. In particular, BAFF, APRIL and CXCL12 are linked to concomitant downregulation of Bim and increased pro-survival expression.27, 28, 29 Furthermore, stromal cell interaction, particularly with follicular dendritic cells, has been identified as a major determinant of survival in CLL and the Eμ-Tcl1 Tg model, via CD44-mediated Mcl-1 upregulation and subsequent apoptotic resistance.48, 49 Cumulatively, these studies suggest a conserved link between BCR, cytokines/chemokines and cellular interactions in the regulation of intrinsic apoptosis and B-cell homeostasis.
Here we showed that PI3Kδi administration inhibited cellular viability (coincident with upregulation of Bim activity) and chemotaxis, alongside inhibition of BAFF-, CXCL12-, APRIL- and follicular dendritic cell-mediated survival pathways. Furthermore, PI3Kδi imparted a Bim-dependent reduction in Eμ-Tcl1 Tg leukemia cells in vivo, associated with increased Bim expression.
Upregulation of Bim has been observed in vitro with other BCR-inducible kinase inhibitors.50, 51 Consequently, upregulation of Bim appears a common mechanism triggered by inhibition of BCR-inducible kinases. Although PI3Kδi-mediated Bim upregulation was evident in vitro in the absence of exogenous antigen, antigen contamination of in vitro cultures from murine tissues is likely. Therefore, it remains unclear whether Bim upregulation occurs via inhibition of the BCR. In diffuse large B-cell lymphoma cell lines, where in vitro antigen-dependent BCR signaling is required for survival,52 PI3Kδi-mediated Bim upregulation was evident in some but not all cell lines. Therefore, removal of BCR-mediated Bim suppression may, at least in part, explain PI3Kδi-mediated Bim upregulation. However, since BAFF and BCR signals appear to co-operate in the maintenance of B-cell populations, via an NF-KB-centered integration node,53 it is likely that PI3Kδi-mediated Bim upregulation occurs as a consequence of inhibition of both BCR- and cytokine/chemokine-mediated pathways.
On the basis of the data reported herein, we propose a mechanism whereby PI3Kδ inhibition imparts therapeutic responses in hematological malignancies by both malignant cell intrinsic and immunomodulatory mechanisms. In the former, PI3Kδ inhibition abrogates BCR-mediated suppression of Bim, reducing the ability of malignant B-cells to survive in the periphery, and increasing reliance on SLO transit and survival networks. However, concurrent PI3Kδi-mediated inhibition of malignant B-cell entry into SLOs, migration to follicular dendritic cell-rich areas and inhibition of SLO-resident pro-survival signals denies this. Cumulatively, these effects result in enhanced Bim expression, enhanced mitochondrial priming and the apoptotic demise of malignant B-cells by a ‘death by neglect’-like process. Similar observations have been reported previously, whereby microenvironmental support reduced mitochondrial priming of lymph node-resident CLL cells, which was reversed on idelalisib administration.17
Although loss of Bim imparted significant in vitro and in vivo resistance toward PI3Kδi, therapeutic responses were not totally ablated. Because only Bcl-2 Tg Eμ-Tcl1 Tg leukemias were completely refractory to PI3Kδi-induced cytotoxicity in vitro, additional BH3-only proteins likely contribute. Indeed, co-operative relationships between Bim and other BH3-only proteins in hematopoiesis and responses to apoptotic stimuli are well documented, including downstream of the BCR.24, 26
In combination with anti-CD20 mAbs, PI3Kδi administration enhanced the duration of leukemia depletion in vivo, in line with clinical trial results.12 This effect was ablated upon genetic loss of Bim. It is likely that the enhanced duration of depletion offered by combinations of anti-CD20 mAbs and PI3Kδi reflect the Bim-mediated reduction of mAb-resistant tumor deposits within SLOs. Indeed, the anatomical distribution of target cells, and their respective access to the hepatic reticuloendothelial system, has been linked to the extent of B-cell depletion within these sites.54 Thus, by reducing SLO-mediated mAb resistance and cellular support mechanisms, PI3Kδi likely enhances anti-CD20 mediated depletion within SLOs and slows the rate of relapse after loss of mAb from the circulation.
Given that cytotoxicity through intrinsic apoptosis appears the primary effector mechanism of PI3Kδi in vivo, synergistic, or at least additive, therapeutic effects may be achieved by combining inhibitors of PI3Kδ and Bcl-2, such as ABT-199 (Venetoclax). This hypothesis is supported by our, and others, observations of enhanced apoptosis when applied in combination.55 Surprisingly, in vivo ABT-199 monotherapy proved ineffective in the treatment of Bim+/+ Eμ-Tcl1 Tg-recipient animals (Figure 8c). In clinical trials, ABT-199 has yielded impressive results in the treatment of relapsed/refractory CLL.56 This apparent ABT-199 resistance is most likely attributable to high levels of Bcl-XL expression (Supplementary Figure 8a), which in CLL has been linked to a 1000-fold reduction in ABT-199 sensitivity.46 In contrast to ABT-199 monotherapy, PI3Kδi:ABT-199 combination therapy reduced in vivo leukemic burden by 95% in comparison to vehicle controls. These effects are likely attributable to enhanced Bim-mediated mitochondrial priming following PI3Kδi application allowing greater ABT-199-mediated displacement of Bim, resulting in enhanced Bax/Bak activation.
This proof-of-concept experiment demonstrates the ability of new mechanistic knowledge to provide rationale-based combination strategies for more effective treatments. Looking forward, inhibitors targeting additional aspects of microenvironmental support, such as IL-4 and CD40L, signaling could be incorporated. These T-cell-mediated microenvironment-derived support signals enhance BCR signaling and generate therapeutic resistance toward Bcl-2 inhibitors.46, 57 Since these pathways exhibit only partial PI3Kδ dependency7 use of an additional JAK:STAT inhibitor may further enhance the efficacy of PI3Kδ:Bcl-2 inhibitor combinations and provide a curative treatment regime.
References
Burger J, Ghia P, Rosenwald A, Caligaris-Cappio F . The microenvironment in mature B-cell malignancies: a target for new treatment strategies. Blood 2009; 114: 3367–3375.
Nore BF, Vargas L, Mohamed AJ, Branden LJ, Backesjo CM, Islam TC et al. Redistribution of Bruton's tyrosine kinase by activation of phosphatidylinositol 3-kinase and Rho-family GTPases. Eur J Immunol 2000; 30: 145–154.
Andjelkovic M, Alessi DR, Meier R, Fernandez A, Lamb NJC, Frech M et al. Role of translocation in the activation and function of protein kinase B. J Biol Chem 1997; 272: 31515–31524.
Vanhaesebroeck B, Whitehead MA, Pineiro R . Molecules in medicine mini-review: isoforms of PI3K in biology and disease. J Mol Med 2016; 94: 5–11.
Bi L, Okabe I, Bernard DJ, Wynshaw-Boris A, Nussbaum RL . Proliferative defect and embryonic lethality in mice homozygous for a deletion in the p110 alpha subunit of phosphoinositide 3-kinase. J Biol Chem 1999; 274: 10963–10968.
Bi L, Okabe I, Bernard DJ, Nussbaum RL . Early embryonic lethality in mice deficient in the p110 beta catalytic subunit of PI 3-kinase. Mamm Genome 2002; 13: 169–172.
Okkenhaug K, Bilancio A, Farjot G, Priddle H, Sancho S, Peskett E et al. Impaired B and T cell antigen receptor signaling in p110 delta PI 3-kinase mutant mice. Science 2002; 297: 1031–1034.
Bilancio A, Okkenhaug K, Camps M, Emery JL, Ruckle T, Rommel C et al. Key role of the p110 delta isoforrn of PI3K in B-cell antigen and IL-4 receptor signaling: comparative analysis of genetic and pharmacologic interference with p110 delta function in B cells. Blood 2006; 107: 642–650.
Soond DR, Bjorgo E, Moltu K, Dale VQ, Patton DT, Torgersen KM et al. PI3K p110 delta regulates T-cell cytokine production during primary and secondary immune responses in mice and humans. Blood 2010; 115: 2203–2213.
Ali K, Soond DR, Pineiro R, Hagemann T, Pearce W, Lim EL et al. Inactivation of PI(3)K p110 delta breaks regulatory T-cell-mediated immune tolerance to cancer. Nature 2014; 510: 407.
Brown JR, Byrd JC, Coutre SE, Benson DM, Flinn IW, Wagner-Johnston ND et al. Idelalisib, an inhibitor of phosphatidylinositol 3-kinase p110 delta, for relapsed/refractory chronic lymphocytic leukemia. Blood 2014; 123: 3390–3397.
Furman RR, Sharman JP, Coutre SE, Cheson BD, Pagel JM, Hillmen P et al. Idelalisib and rituximab in relapsed chronic lymphocytic leukemia. N Engl J Med 2014; 370: 997–1007.
Woyach JA, Furman RR, Liu TM, Ozer HG, Zapatka M, Ruppert AS et al. Resistance mechanisms for the bruton's tyrosine kinase inhibitor ibrutinib. N Engl J Med 2014; 370: 2286–2294.
Hoellenriegel J, Meadows SA, Sivina M, Wierda WG, Kantarjian H, Keating MJ et al. The phosphoinositide 3 '-kinase delta inhibitor, CAL-101, inhibits B-cell receptor signaling and chemokine networks in chronic lymphocytic leukemia. Blood 2011; 118: 3603–3612.
Herman SEM, Gordon AL, Wagner AJ, Heerema NA, Zhao W, Flynn JM et al. Phosphatidylinositol 3-kinase-delta inhibitor CAL-101 shows promising preclinical activity in chronic lymphocytic leukemia by antagonizing intrinsic and extrinsic cellular survival signals. Blood 2010; 116: 2078–2088.
Fiorcari S, Brown WS, McIntyre BW, Estrov Z, Maffei R, O'Brien S et al. The PI3-kinase delta inhibitor idelalisib (gs-1101) targets integrin-mediated adhesion of chronic lymphocytic leukemia (CLL) cell to endothelial and marrow stromal cells. Plos One 2013; 8: e83830.
Davids MS, Deng J, Wiestner A, Lannutti BJ, Wang L, Wu CJ et al. Decreased mitochondrial apoptotic priming underlies stroma-mediated treatment resistance in chronic lymphocytic leukemia. Blood 2012; 120: 3501–3509.
Lannutti BJ, Meadows SA, Herman SEM, Kashishian A, Steiner B, Johnson AJ et al. CAL-101, a p110 delta selective phosphatidylinositol-3-kinase inhibitor for the treatment of B-cell malignancies, inhibits PI3K signaling and cellular viability. Blood 2011; 117: 591–594.
Czabotar PE, Lessene G, Strasser A, Adams JM . Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol Cell Biol 2014; 15: 49–63.
Letai A, Bassik MC, Walensky LD, Sorcinelli MD, Weiler S, Korsmeyer SJ . Distinct BH3 domains either sensitize or activate mitochondrial apoptosis, serving as prototype cancer therapeutics. Cancer Cell 2002; 2: 183–192.
Llambi F, Moldoveanu T, Tait SWG, Bouchier-Hayes L, Temirov J, McCormick LL et al. A Unified Model of Mammalian BCL-2 Protein Family Interactions at the Mitochondria. Mol Cell 2011; 44: 517–531.
Enders A, Bouillet P, Puthalakath H, Xu YK, Tarlinton DM, Strasser A . Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim inhibits BCR stimulation-induced apoptosis and deletion of autoreactive B cells. J Exp Med 2003; 198: 1119–1126.
Bouillet P, Metcalf D, Huang DCS, Tarlinton DM, Kay TWH, Kontgen F et al. Proapoptotic Bcl-2 relative bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity. Science 1999; 286: 1735–1738.
Carter MJ, Cox KL, Blakemore SJ, Bogdanov YD, Happo L, Scott CL et al. BCR-signaling-induced cell death demonstrates dependency on multiple BH3-only proteins in a murine model of B-cell lymphoma. Cell Death Differ 2016; 23: 303–312.
Srinivasan L, Sasaki Y, Calado DP, Zhang B, Paik JH, DePinho RA et al. PI3 kinase signals BCR-dependent mature B cell survival. Cell 2009; 139: 573–586.
Woess C, Tuzlak S, Labi V, Drach M, Bertele D, Schneider P et al. Combined loss of the BH3-only proteins Bim and Bmf restores B-cell development and function in TACI-Ig transgenic mice. Cell Death Differ 2015; 22: 1477–1488.
Craxton A, Draves KE, Gruppi A, Clark EA . BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway. J Exp Med 2005; 202: 1363–1374.
Trushin SA, Carena AA, Bren GD, Rizza SA, Dong X, Abraham RS et al. SDF-1 alpha degrades whereas glycoprotein 120 upregulates Bcl-2 interacting mediator of death extralong isoform: implications for the development of T cell memory. J Immunol 2012; 189: 1835–1842.
Enzler T, Kater AP, Zhang W, Widhopf GF II, Chuang H-Y, Lee J et al. Chronic lymphocytic leukemia of E mu-TCL1 transgenic mice undergoes rapid cell turnover that can be offset by extrinsic CD257 to accelerate disease progression. Blood 2009; 114: 4469–4476.
Shugg RPP, Thomson A, Tanabe N, Kashishian A, Steiner BH, Puri KD et al. Effects of isoform-selective phosphatidylinositol 3-kinase inhibitors on osteoclasts actions on cytoskeletal organisation, survival, and resorption. J Biol Chem 2013; 288: 35346–35357.
Hallek M, Cheson BD, Catovsky D, Caligaris-Cappio F, Dighiero G, Doehner H et al. Guidelines for the diagnosis and treatment of chronic lymphocytic leukemia: a report from the International Workshop on Chronic Lymphocytic Leukemia updating the National Cancer Institute-Working Group 1996 guidelines. Blood 2008; 111: 5446–5456.
Lim SH, Vaughan AT, Ashton-Key M, Williams EL, Dixon SV, Chan HTC et al. Fc gamma receptor IIb on target B cells promotes rituximab internalization and reduces clinical efficacy. Blood 2011; 118: 2530–2540.
Bichi R, Shinton SA, Martin ES, Koval A, Calin GA, Cesari R et al. Human chronic lymphocytic leukemia modeled in mouse by targeted TCL1 expression. Proc Natl Acad Sci USA 2002; 99: 6955–6960.
Bresin A, D'Abundo L, Narducci MG, Fiorenza MT, Croce CM, Negrini M et al. TCL1 transgenic mouse model as a tool for the study of therapeutic targets and microenvironment in human B-cell chronic lymphocytic leukemia. Cell Death Dis 2016; 7: e2071.
Johnson AJ, Lucas DM, Muthusamy N, Smith LL, Edwards RB, De lay MD et al. Characterization of the TCL-1 transgenic mouse as a preclinical drug development tool for human chronic lymphocytic leukemia. Blood 2006; 108: 1334–1338.
DiLillo DJ, Weinberg JB, Yoshizaki A, Horikawa M, Bryant JM, Iwata Y et al. Chronic lymphocytic leukemia and regulatory B cells share IL-10 competence and immunosuppressive function. Leukemia 2013; 27: 170–182.
Kater AP, Tonino SH, Kersten MJ, Hagenbeek A, Spiering M, van Oers MH et al. Interim analysis of dose-escalation stage of a phase 1b study evaluating safety and pharmacology of gs-9820, a second-generation, selective, pi3kd-inhibitor in recurrent lymphoid malignancies. Blood 2013; 122: 2881.
de Rooij MFM, Kuil A, Geest CR, Eldering E, Chang BY, Buggy JJ et al. The clinically active BTK inhibitor PCI-32765 targets B-cell receptor- and chemokine-controlled adhesion and migration in chronic lymphocytic leukemia. Blood 2012; 119: 2590–2594.
Buchner M, Baer C, Prinz G, Dierks C, Burger M, Zenz T et al. Spleen tyrosine kinase inhibition prevents chemokine- and integrin-mediated stromal protective effects in chronic lymphocytic leukemia. Blood 2010; 115: 4497–4506.
Certo M, Moore VD, Nishino M, Wei G, Korsmeyer S, Armstrong SA et al. Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members. Cancer Cell 2006; 9: 351–365.
Nakano K, Vousden KH . PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell 2001; 7: 683–694.
Egle A, Harris AW, Bouillet P, Cory S . Bim is a suppressor of Myc-induced mouse B cell leukemia. Proc Natl Acad Sci USA 2004; 101: 6164–6169.
Richter-Larrea JA, Robles EF, Fresquet V, Beltran E, Rullan AJ, Agirre X et al. Reversion of epigenetically mediated BIM silencing overcomes chemoresistance in Burkitt lymphoma. Blood 2010; 116: 2531–2542.
Sinicrope FA, Rego RL, Okumura K, Foster NR, O'Connell MJ, Sargent DJ et al. Prognostic impact of Bim, Puma, and Noxa expression in human colon carcinomas. Clin Cancer Res 2008; 14: 5810–5818.
Beers SA, French RR, Chan CH, Lim SH, Jarrett TC, Mora Vidal R et al. Antigenic modulation limits the efficacy of anti-CD20 antibodies: implications for antibody selection. Blood 2010; 115: 5191–5201.
Vogler M, Butterworth M, Majid A, Walewska RJ, Sun X-M, Dyer MJS et al. Concurrent up-regulation of BCL-X(L) and BCL2A1 induces approximately 1000-fold resistance to ABT-737 in chronic lymphocytic leukemia. Blood 2009; 113: 4403–4413.
Paterson A, Mockridge CI, Adams JE, Krysov S, Potter KN, Duncombe AS et al. Mechanisms and clinical significance of BIM phosphorylation in chronic lymphocytic leukemia. Blood 2012; 119: 1726–1736.
Pedersen IM, Kitada S, Leoni LM, Zapata JM, Karras JG, Tsukada N et al. Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1. Blood 2002; 100: 1795–1801.
Heinig K, Gaetjen M, Grau M, Stache V, Anagnostopoulos I, Gerlach K et al. Access to follicular dendritic cells is a pivotal step in murine chronic lymphocytic leukemia B-cell activation and proliferation. Cancer Discov 2014; 4: 1448–1465.
Szydlowski M, Kiliszek P, Sewastianik T, Jablonska E, Bialopiotrowicz E, Gorniak P et al. FOXO1 activation is an effector of SYK and AKT inhibition in tonic BCR signal-dependent diffuse large B-cell lymphomas. Blood 2016; 127: 739–748.
Rahmani M, Anderson A, Habibi JR, Crabtree TR, Mayo M, Harada H et al. The BH3-only protein Bim plays a critical role in leukemia cell death triggered by concomitant inhibition of the PI3K/Akt and MEK/ERK1/2 pathways. Blood 2009; 114: 4507–4516.
Young RM, Wu T, Schmitz R, Dawood M, Xiao W, Phelan JD et al. Survival of human lymphoma cells requires B-cell receptor engagement by self-antigens. Proc Natl Acad Sci USA 2015; 112: 13447–13454.
Stadanlick JE, Kaileh M, Karnell FG, Scholz JL, Miller JP, Quinn WJ III et al. Tonic B cell antigen receptor signals supply an NF-kappa B substrate for prosurvival BLyS signaling. Nat Immunol 2008; 9: 1379–1387.
Gong Q, Ou QL, Ye SM, Lee WP, Cornelius J, Diehl L et al. Importance of cellular microenvironment and circulatory dynamics in B cell immunotherapy. J Immunol 2005; 174: 817–826.
Choudhary GS, Al-Harbi S, Mazumder S, Hill BT, Smith MR, Bodo J et al. MCL-1 and BCL-xL-dependent resistance to the BCL-2 inhibitor ABT-199 can be overcome by preventing PI3K/AKT/mTOR activation in lymphoid malignancies. Cell Death Dis 2015; 6: e1593.
Seymour JF, Davids MS, Pagel JM, Kahl BS, Wierda WG, Puvvada S et al. ABT-199 (GDC-0199) in relapsed/refractory chronic lymphocytic leukemia and small lymphocytic lymphoma: high response rates among patients with high risk disease features including unmated IgHV. Haematologica 2014; 99: 249–249.
Aguilar-Hernandez MM, Blunt MD, Dobson R, Yeomans A, Thirdborough S, Larrayoz M et al. IL-4 enhances expression and function of surface IgM in CLL cells. Blood 2016; 127: 3015–3025.
Acknowledgements
The authors gratefully acknowledge all patients who contributed the samples used in this study. We are grateful to the Experimental Cancer Medicine Centre funded University of Southampton, Faculty of Medicine Human Tissue Bank (Human Tissue Authority license 12009) and to I Tracy, I Henderson and KN Potter for the assistance with collecting and characterizing clinical material. The authors thank Christophe Quéva for advice during experimental design. Cell lines were the generous gift of (HK) Yong Sung Choi, (OCI-LY10) Alison Banham and (HBL-1) Martin Dyer. Anti-mouse p53 antibody was kindly provided by Giovanna Roncador. Funding was provided by Kay Kendall Leukaemia Fund (687), Bloodwise (Grants 10012, 12050) and Cancer Research UK (C34999/A18087, ECMC C24563/A15581).
Author contributions
MJC performed research, analyzed and interpreted data and wrote the manuscript; KLC and AHT assisted with in vivo experiments; LND performed in vitro experiments with primary human CLL samples; SJB and RJO aided in characterization of in vivo models; ST offered guidance with experimental planning and provided GS-9820; FF provided primary human CLL samples; GKP helped design research, analyzed data and edited the manuscript; MSC designed research, analyzed and interpreted data and wrote the manuscript with MJC.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
MSC serves as a consultant for Bioinvent International and has previously served as an ad hoc consultant for Roche and Baxalta. He has previously received grant funding from Bioinvent, Roche, Gilead and GSK. GP receives research funding from Aquinox Pharmaceuticals and is a founder and shareholder in Karus Therapeutics.
Additional information
Supplementary Information accompanies this paper on the Leukemia website
Supplementary information
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Carter, M., Cox, K., Blakemore, S. et al. PI3Kδ inhibition elicits anti-leukemic effects through Bim-dependent apoptosis. Leukemia 31, 1423–1433 (2017). https://doi.org/10.1038/leu.2016.333
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/leu.2016.333
- Springer Nature Limited
This article is cited by
-
Selective PI3Kδ inhibitor TYM-3-98 suppresses AKT/mTOR/SREBP1-mediated lipogenesis and promotes ferroptosis in KRAS-mutant colorectal cancer
Cell Death & Disease (2024)
-
Anti-tumor activity of selinexor in combination with antineoplastic agents in chronic lymphocytic leukemia
Scientific Reports (2023)
-
Potential mechanisms of resistance to venetoclax and strategies to circumvent it
BMC Cancer (2017)