Abstract
Affective memories associated with the negative emotional state experienced during opiate withdrawal are central in maintaining drug taking, seeking, and relapse. Nucleus accumbens (NAC) is a key structure for both acute withdrawal and withdrawal memories reactivation, but the NAC neuron coding properties underpinning the expression of these memories remain largely unknown. Here we aimed at deciphering the role of NAC neurons in the encoding and retrieval of opiate withdrawal memory. Chronic single neuron and local field potentials recordings were performed in morphine-dependent rats and placebo controls. Animals were subjected to an unbiased conditioned placed aversion protocol with one compartment (CS+) paired with naloxone-precipitated withdrawal, a second compartment with saline injection (CS−), and a third being neutral (no pairing). After conditioning, animals displayed a typical place aversion for CS+ and developed a preference for CS− characteristic of safety learning. We found that distinct NAC neurons code for CS+ or CS−. Both populations also displayed highly specific oscillatory dynamics, CS+ and CS− neurons, respectively, following 80 Hz (G80) and 60 Hz (G60) local field potential gamma rhythms. Finally, we found that the balance between G60 and G80 rhythms strongly correlated both with the ongoing behavior of the animal and the strength of the conditioning. We demonstrate here that the aversive and preferred environments are underpinned by distinct groups of NAC neurons as well as specific oscillatory dynamics. This suggest that G60/G80 interplay—established through the conditioning process—serves as a robust and versatile mechanism for a fine coding of the environment emotional weight.
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INTRODUCTION
Through daily life experience, we learn to associate contexts and stimuli with emotions bearing positive or negative values. These affective memories underlie our ability to adapt to appetitive and aversive situations and play a crucial role in pathologies such as addiction. Indeed, conditioned drug-like and drug-opposite (eg, withdrawal) effects have been shown to play a crucial role in the development and maintenance of compulsive drug use (Milton and Everitt, 2012; Torregrossa et al, 2011). Opiate abuse in particular (heroin, morphine) induces a strong dependence that is characterized by the appearance of a withdrawal syndrome upon drug use cessation (Frenois et al, 2005a). Opiate withdrawal can induce a mild to severe somatic syndrome together with the formation of long-lasting aversive memories driven by the association of stimuli, contexts, and emotions related to withdrawal itself (Frenois et al, 2005a). In parallel to these negative associative memories, morphine-dependent subjects can also distinguish cues and contexts that consistently signal the absence of withdrawal and form associative memories based on these perceived safety signals (Caille et al, 2003; Leknes et al, 2011). Such emotional memories are considered as a key component in the addiction process in that they motivate drug seeking and further promote maladaptive behaviors such as sustained drug taking or relapse in abstinent subjects (Milton and Everitt, 2012; Torregrossa et al, 2011). Moreover, preclinical studies have shown that reactivation of affective memories associated with opiate withdrawal state can reinstate drug seeking (Hellemans et al, 2006a, 2006b; Kenny et al, 2006).
The nucleus accumbens (NAC) is a ‘limbic-motor interface’ that underpins the production of adaptive behavioral responses (Carlezon and Thomas, 2009). The NAC subserves addictive processes by allowing the formation of specific representations of the sensory, emotional, and motivational properties of drug-associated stimuli or contexts that contribute to drive maladaptive behaviors characterizing addiction (Cador et al, 1989; Robbins et al, 1989). Interestingly NAC is at stake for both the rewarding effects of opiates (Kelsey et al, 1989; Olds, 1982) and the aversive effects of withdrawal (Frenois et al, 2002, 2005a, 2005b; Koob et al, 1989; Stinus et al, 1990). NAC neuron differential activity has been linked to both reward and aversion (see, eg, Roitman et al, 2010; Setlow et al, 2003), but little is known about the NAC precise coding of positive and negative in the frame of opiate dependence and withdrawal. Rate coding has been proposed as a model of dual coding for drug-related safety and aversion with tonic inhibition or excitation respectively (for review, see Carlezon and Thomas, 2009). Alternative mechanisms like neural codes based on spike train dynamics such as temporal or phase coding have been largely overlooked in the frame of opiate addiction. Synchronous theta and 4 Hz oscillatory activity in the limbic system is associated with successful reward retrieval (Benchenane et al, 2010; Fujisawa and Buzsaki, 2011) and most importantly in associative aversive memories, prefrontal cortex-amygdalar theta and 4 Hz oscillatory synchronization are instrumental for fear expression (Courtin et al, 2014; Dejean et al, 2016; Karalis et al, 2016). Oscillatory activity in the NAC and striatum and most particularly in the gamma band (40–120 Hz) is entrained by prefrontal and amygdalar oscillatory inputs (Donnelly et al, 2014; von Nicolai et al, 2014). Hence, beside rate coding, oscillatory synchronization in the gamma band appears as strong candidate mechanism for the behavioral expression of drug-related memories. In line with this idea, we have recently shown that chronic morphine and withdrawal were associated with specific modifications in NAC gamma band oscillations (40–120 Hz) in rodents (Dejean et al, 2013). In both morphine-dependent and -naive animals, these gamma oscillations split into two distinct functional frequency bands that have also been identified as candidate mechanisms in different memory-based paradigms (Headley and Pare, 2013; van der Meer et al, 2010). A specific increase in 60 Hz low gamma (G60) is observed during acute and chronic opiate stimulation, whereas 80 Hz high gamma (G80) is upregulated at the time of acute withdrawal (Dejean et al, 2013).
As G60 and G80 oscillations are acutely modulated by opiates and withdrawal (Dejean et al, 2013), the present work investigates their role in the expression of specific behaviors related to opiate withdrawal memories: (1) withdrawal itself but also (2) the preference associated with the absence of withdrawal. Using single unit and local field potential (LFP) recordings in a conditioned place aversion paradigm in behaving rats, we show here that aversion and preference are correlated with a differential activity of NAC neurons, and that in both spike trains and LFP, this functional scheme emerges from specific interactions between G60 and G80.
MATERIALS AND METHODS
Animals
Twelve male Sprague-Dawley rats (Charles River Laboratories, France) were individually housed under an inverted 12 h light/dark cycle at 21±2 °C with food and water available ad libitum. Weight varied from 250 to 300 g at the beginning of the study to 300 to 350 g at the time of the last recording. Surgical and experimental procedures were performed in accordance with the European Community’s Council Directive (EU Directive 2010/63/EU86) for the care and use of laboratory animals. The present experiment was approved by the local animal care and use committee (approval 5012049-A).
Electrode Implantation Surgery
Rats were implanted with four independently moveable tetrodes in the NAC as described in Dejean et al (2013). Briefly, surgery took place after at least 7 days of daily handling, under full general gas anesthesia (Isoflurane 1.5–2%). The recording electrodes were lowered to position through small craniotomies above the structure. Six stainless-steel skull screws were inserted and the implant was affixed to the skull with super bond and dental acrylic. After the surgery, animals were injected with carboprofen (2 mg/kg, s.c.) for pain management and allowed to recover from anesthesia before being returned to the animal housing facilities where pain management was carried on for 5–7 days.
Behavioral Paradigm: Opiate Withdrawal Place Aversion Conditioning
Following the first surgery, morphine dependence was induced by subcutaneous implantation of two morphine (n=7) or placebo (n=5) pellets as previously described (Dejean et al, 2013). Withdrawal was precipitated by a naloxone injection (15 μg/kg s.c.) that induced a strong aversive state with mild somatic signs (Frenois et al, 2002). Place aversion conditioning took place in an unbiased Y-maze with three compartments as previously described in detail (Frenois et al, 2005b) (see also Figure 1a). Following a pretest, animals underwent conditioning during that rats received a saline injection on days 5, 7, and 9, and a naloxone injection (15 μg/kg) on alternate days (6, 8, and 10) before being placed in one compartment of the Y-maze for 20 min. The testing phase (test) involved a free Y-maze exploration for 20 min and a place aversion score was calculated as the difference between the percentage of time spent in the same compartment during test and pretest. The occurrence of some withdrawal signs (defecation and diarrhea) were analyzed by a visual inspection of the video recording. For a detailed description of the paradigm, see Supplementary Methods.
Behavioral and Electrophysiological Recordings
Behavior was monitored with a videotracking system (Plexon, TX, USA). NAC single units and LFP were recorded using Multichannel Acquisition Processor (MAP, Plexon). The wide band signal (0.1–9000 Hz) collected by the electrodes was amplified (× 1000) before being digitized (40000 Hz sampling rate for single unit and 1000 Hz for LFP). Single units were manually sorted and cluster isolation was tested for significance (Offline sorter, Plexon). Units were classified as putative projection neurons or interneurons (Supplementary Figure S2a) using combined spike width and firing rate methods as previously described (Dejean et al, 2013). Behavioral and electrophysiological activities were monitored daily from pretest to test. Some animals displayed brief sleep episodes by the end of the pretest period. Although these events were sparse, for the sake of rigor and to avoid such epochs to influence our analysis, we isolated and removed those episodes showing behavioral (eg, prolonged immobility) and electrophysiological hallmarks of sleep (eg, changes in LFP slow oscillatory regime).
Electrophysiological Data Analysis
Frequency firing maps were computed combining spike sorting and tracking results. An image containing the Y-maze was divided into 1024 pixels (32 × 32). Sorted spikes were distributed in the maze space according to the animal position at the time of firing. The firing rate in each pixel was calculated as the ratio of the number of spikes fired inside the pixel over the time spent by the animal in the same pixel along the entire session (Figures 1d and e). Firing was Z-scored and pixels showing a significantly Z-score (>1.96) and six contiguous significant pixels defined a firing field. Neurons displaying one or more clusters in a single compartment of the maze were tagged as context neurons.
The phase relationship between spikes and LFP was investigated using phase histograms. For each rhythm of interest, LFP were band pass filtered in Matlab and the phase of filtered signals was extracted by calculating the Hilbert transform of the signal in Matlab (The Mathworks, MA, USA). Spike-phase histograms were tested for homogeneity (Rayleigh test), with a nonhomogeneous histogram indicating significant phase locking. LFP power spectrum analyses revealed an overlap between G60 and G80 event frequency bands. To overcome this problem and perform a specific analysis of these two types of events, each single gamma event was precisely isolated and classified as G60 or G80 (see Supplementary Methods and Supplementary Figure S3). To predict the animal position based on the presence of G60 and G80 events in the LFP, we used a machine learning approach and a naive Bayes classifier and assessed prediction accuracy through ROC analysis (see Supplementary Methods).
Statistics
For each statistical analysis, Kolmogorov–Smirnov normality test was first performed. To calculate the appropriate sample size, power analyses were carried out to determine the number of rats needed (Sigmaplot, Systat, Germany) using a power >0.8 and a type I error threshold α=0.05. Two-sided parametric statistical tests were used in case data met normality and homoscedasticity. In case one or both of these conditions were not fulfilled, or the sample size was small (5 or 6), nonparametric methods were used. On one occasion, equal variance criteria were not met and no nonparametric alternative was available to our knowledge (Figure 4b). These results are thus only provided for information. In case of multiple comparisons, adjustments were systematically done to control for family errors (Holm–Sidak and Tukey’s post hoc tests for parametric and nonparametric statistics respectively). Experimenter blinding (single blind) was used on one occasion: the experimenter extracting the firing fields of neurons in the maze had no knowledge of the animal ID or the condition (test or pretest). On graphics, error bars are mean±SEM unless otherwise stated.
RESULTS
NAC Neuron Firing Correlates with Negative and Positive Emotional Contexts after Place Aversion Conditioning
Unbiased place conditioning resulted in a marked aversion toward the withdrawal-associated compartment (CS+, Figures 1b and c). Interestingly, every animal also developed a preference for the saline paired compartment (CS−, Figures 1b and c), whereas the time spent in neutral compartment (N) remained unchanged. In placebo animals the time spent in compartments in test and pretest did not change (Supplementary Figure S1a). Hence, familiarity regarding CS+ and CS− acquired during conditioning does not explain test behavior in morphine animals nor can saline injection explain the preference toward CS−. This shows that place conditioning, in addition to allocating a negative emotional weight to the CS+, also established the maze as a robust bivalent emotional environment with CS− becoming a positively perceived compartment. Moreover, the investigation of motion statistics revealed that the distribution of animal speeds remained unchanged between pretest and test in placebo or morphine animals (Supplementary Figure S1d). Similarly, the total distance traveled during a session was unaffected by the experimental group or the conditioning (Supplementary Figure S1e). NAC is crucial for the association of actions with their values. We therefore investigated the electrophysiological correlates of NAC cells with the different contexts and most particularly the CS+ and CS−. We focused on putative medium spiny projection neurons (Supplementary Figure S2) and recorded and analyzed 258 cells (pretest: n=53; conditioning: n=167; test n=38). In pretest, activity of most neurons was evenly distributed in space with firing fields observed in two or three compartments (79%; 42/53, Figure 1d). During test a majority of cells started to fire specifically in one of the three emotional contexts (50%, 19/38, Figure 1e). Among them similar amounts presented firing fields restricted to CS+, CS− or N (5/19, 6/19, and 8/19, respectively). Note that at the population level, average firing rate was not found to be modulated by conditioning, NAC substructure (core or shell), or compartment (Supplementary Figure S2d). In placebo animals (Supplementary Figures S2e and f), NAC presented similar proportions of context neurons in pretest (4/19, 21%) and test (7/29, 24%). When comparing with morphine animals, these values did not differ from pretest but were significantly lower than that observed during test (Fisher’s exact test, placebo pretest vs morphine pretest: p=0.94; placebo test vs morphine pretest: p=1; placebo pretest vs morphine test: p<0.05; placebo test vs morphine test: p<0.05). These data correlate with a switch from a weakly emotional context in pretest to the emotional polarization of the maze observed after conditioning (test).
Slow and Fast Gamma Event Relative Weight Correlates with Preference and Aversion
The analysis of LFP revealed a striking homology between the animals’ preference or aversion behavior and gamma band oscillations. Gamma oscillation events occurred under the form of distinct spindles that could be classified into two categories based on their main frequency component (Figure 2a and Supplementary Figures S3c and d): the G60 and G80 rhythms with power peaking near 60 and 80 Hz, respectively. These rhythms have been observed in both morphine and drug-free subjects (Dejean et al, 2013; van der Meer et al, 2010) and, strikingly, in our paradigm their relative prevalence was tightly linked to the animal’s behavior. During epochs spent in aversive CS+ G60 event probability and duration decreased, whereas G80 probability increased (Figures 2b and c and Supplementary Figures S3e and S4a). In the preferred CS− instead, G60 events were strongly promoted and both their duration and power increased compared with pretest (Figures 2b and c,Supplementary Figures S3e and S4a, c). Interestingly, no changes were observed in N for either frequency component. These opposed yet concurrent changes in G60 and G80 suggest that both rhythms are involved in the coding of the compartment emotional value. Further analysis revealed that the probability to observe a G60 event turned out to be higher after the occurrence of a G80 event (Figure 2d) and power was significantly increased from one G60 event to the next when a G80 event occurred in between them (Figure 2e). Conversely, the probability to observe a G80 event was lower after the occurrence of a G60 event and power decreased from one G80 event to the next with an intercalated G60 events (Figure 2e). These results suggest a possible fast interaction between G60 and G80. In line with this, cross-frequency coupling analysis at the scale of an entire recording session shows that power of ∼60 Hz was negatively correlated with that ∼80 Hz (Figure 2f). Interestingly, this negative correlation was strengthened by conditioning (pretest rho=−0.069±0.015, test: rho=−0.196±0.0349, paired t-test, t(6)=3.763, p<0.01). Recent works have emphasized that NAC gamma rhythms correlate with the animal’s motoric behavior (Malhotra et al, 2015). In our experiment, although G60 and G80 events were indeed found to be correlated with rats’ motoric activity (Supplementary Figures S5a–c), their fluctuations were significantly better explained by their mutual interactions (Supplementary Figures S5a–c). Moreover, at the level of a whole session, gamma ratio was not correlated to the motoric activity of the animal (Supplementary Figure S5d). Altogether, these findings show a strong relationship between task conditioning and G60 and G80 event probabilities. The strengthening between pretest and test phase suggests that more than events themselves, their relative prevalence could be a marker of the context emotional value. To quantify this relationship in our task, we calculated the ratio between G60 and G80 event probabilities in the different compartments (Figure 3a) and quantified the change in gamma ratio between pretest and test phases (Figure 3b). In pretest phase, gamma event ratio was similar in all three compartments. During the test, gamma ratio decreased in CS+ and increased in CS− (Figures 3a and b), whereas no change could be observed in N. Gamma event ratio is therefore linked to the animal emotional state during the test session. As aversion, preference, and emotional neutrality are associated with specific compartments after conditioning, we hypothesized that the relative weight of G60 and G80 events at a given time during test should be predictive of the animal’s location. To test this hypothesis we applied a spatial decoding algorithm based on the relative presence of G60 and G80 events inside discrete time bins and found that the animal’s location could be predicted with significant success in any of the three compartments (Figures 3c–e). Thus, in the NAC of morphine-dependent animals, G60 and G80 events—and most specifically their relative prevalence—stand as a potential vector underlying aversion and preference associated with opiate withdrawal and its absence, respectively.
Neurons Firing for Aversion and Preference Are Locked to Specific LFP Gamma Rhythms
The modulation of neuronal activity by G60 or G80 proved to be a robust marker of preference or aversion behaviors in our task. Joint spike–LFP analysis revealed that context neuron spiking inside the neuron firing field were often coincidental with the occurrence of G80 events for CS+ neurons (eg, Figure 4a) or G60 events for CS− neurons (eg, Figure 4b). The quantification of gamma ratio inside CS+ and CS− neuron firing fields confirmed those observations as CS+ neurons displayed a significantly lower ratio in firing field compared with CS− neurons (eg, Figure 4c). Context neuron firing was not only coincidental with gamma events occurrence as their fine temporal dynamics were also modulated according to gamma rhythm (Figure 4d). In pretest and test, several units phase locked on G60 (pretest: 27/53, test: 21/38) and/or G80 (pretest: 16/53, test: 15/38, Figures 4d–f). Surprisingly, despite changes observed in LFP gamma power, G60 and G80 overall phase locking did not differ in pretest and test. However, when considering specifically the subpopulation of context neurons characterized during test phase, G80 phase locking was highly specific of CS+ neurons inside the CS+ compartment (Figures 4d and g), mirroring the increase in G80 event occurrence and the decrease of G60/G80 ratio that indicates G80 prevalence. Conversely, CS− neurons preferentially locked on G60 oscillations while the animal was inside CS− (Figure 4g), matching the increase in both G60 event occurrence and gamma ratio. Although N neurons displayed significant G60 or G80 phase locking, we did not observe any preferred phase locking toward one or the other rhythm (Figure 4g). Spike–LFP analyses hence revealed a tight binding between context neurons, their preferred compartment, and gamma oscillations with a clear bimodal pattern related to emotional values: aversion and preference are associated with G80 and G60, respectively.
NAC Gamma Events in Aversive and Preferred Compartments Correlate with the Strength of Associative Learning
Because G60 and G80 may, depending on their balance, code either positive or negative emotional states, we hypothesized that the relative weight of G60 and G80 rates at a given location of the maze (referred to as gamma state from here) in NAC represents the net emotional value of this location. To test this postulate, we first compared gamma states in CS+ and CS−. During pretest, CS+ and CS− gamma states were largely overlapping (Figure 5a), whereas in conditioned animals CS+ and CS− gamma states represented significantly isolated clusters (Figure 5a). Consequently, the average Mahalanobis distance between CS+ and CS− clusters was significantly larger in test phase (Figure 5b). We then quantified the magnitude of CS+ and CS− gamma state isolation by calculating the significant part of their Mahalanobis distance that is the distance between CS+ and CS− confidence intervals. Gamma state isolation was used as an index for the strength of emotional coding and compared with the strength of the conditioning (net emotional value, see Materials and Methods). We found a striking positive correlation between gamma state isolation and the net emotional value of the maze (Figure 5c), suggesting that the strength of bidirectional gamma activity is a direct reflection of the strength of emotional associative learning.
Establishment of Aversion and Preference Coding Through Gamma Oscillations During Conditioning
During conditioning the experience of acute withdrawal in CS+ is aversive per se as confirmed by the presence of typical withdrawal signs during naloxone pairings such as enhanced defecations and diarrhea (Figure 6a). Interestingly, diarrhea occurrence (a hallmark of withdrawal signs) also increased during conditioning suggesting a sensitization to repeated naloxone injections (Figure 6a). However, it is not clear how CS− acquires its positive emotional value and how electrophysiological correlates of preference develop during conditioning. A general assumption is that through consecutive CS+ and CS− pairings a subject progressively learns to associate CS− with the absence of negative outcome (safety learning). Based on this, we postulated that safety coding through gamma event ratio profile should develop throughout the conditioning phase, whereas aversion coding should be present from the first naloxone-CS+ pairing. To test this, we analyzed LFP gamma events (Figures 6b and c) in the three consecutive CS+ and CS− pairing sessions along the conditioning phase (see protocol, Figure 1a). The investigation of electrophysiological data across CS+ pairings revealed a decrease in gamma ratio compared with pretest (Figure 6b) that was immediate (pairing 1, P1) and conserved in subsequent pairings. Conversely, confinement in the CS− resulted in a progressive increase in gamma ratio (Figure 6c) that was found significant only by P3. Importantly, these changes in gamma ratio were mirrored by changes in gamma phase locking of single neurons (Supplementary Figure S6). Overall, these results confirm that aversion-related gamma pattern is present from the first pairing of CS+ with naloxone withdrawal, whereas the preference-related pattern for its part is absent in the first stages of conditioning and emerges from the repetition of CS−/saline and CS+/naloxone associations.
DISCUSSION
The present study shed light on NAC neuronal activity that could underlie the expression of aversion and preference for opiate-related emotional memories. The interest of this question stems from the crucial role of those memories in drug seeking and relapse, and the involvement of NAC in their behavioral expression (Koob and Volkow, 2010; Milton and Everitt, 2012). Our work shows that although the context associated with drug withdrawal is perceived as aversive as expected, a second context specifically characterized by the absence of withdrawal can be progressively perceived as positive. During test, at the time these emotional memories are expressed, distinct groups of NAC neurons and specific oscillatory dynamics correlate with the position of the animal in the aversive or preferred environment. Indeed, the firing pattern of neurons underlying preference and aversion are driven by slow (G60) and fast (G80) gamma band oscillations, respectively. Finally, the relative weight and the moment-to-moment interactions between both rhythms precisely define the emotional value allocated to discrete locations of the environment through the conditioning process.
A substantial amount of NAC neurons displayed spatial and emotional information in their spike trains. Hence, just as in drug-naive animals (Hayes et al, 2014; Khamassi et al, 2008; Lansink et al, 2012; Schmitzer-Torbert et al, 2005), single NAC medium spiny neurons encode spatial and emotional traits of the opiate-dependent animal’s context. Importantly, distinct neuronal subpopulations were found to match aversive and preferred locations. This functional segregation shows that the NAC encodes both aversion and preference in dependent-animals just as in drug-naive subjects as evidenced with associative learning based on naturally aversive or rewarding stimuli in both rodents (Roitman et al, 2010) and humans (Levita et al, 2009). Interestingly, a recent work has highlighted that different populations of dynorphin cells in the dorsal or ventral part of the NAC shell may code for preference and aversion, respectively (Al-Hasani et al, 2015). Even if our recordings span across both core and shell, and are mainly dorsal, our data further support the idea that neuronal subpopulations of the NAC may specifically underlie place conditioning for positive and negative emotional values. Thus, NAC activity may subserve representations of contextual elements that are crucial for adapted behaviors (Lansink et al, 2012; Schmitzer-Torbert et al, 2005) including spatiotemporal features and emotional traits whichever their value. In the frame of addiction, such functional flexibility may be required to drive behavior (1) towards the drug and its rewarding properties, (2) towards a beacon of safety, or (3) away from contexts associated with negative aspects of drug abuse (eg, withdrawal). Our paradigm models these last two aspects but cannot directly address questions related to the substance rewarding properties. As our chronic morphine treatment induced tolerance (Gold et al, 1994), it is likely that preference for the CS− compartment (also previously shown in Caille et al, 2003 with the same naloxone dose) develops with the progressive perception of the absence of withdrawal as an increasing safety signal. Preclinical studies have shown that conditioned opiate withdrawal reinstate morphine conditioned place preference (Lu et al, 2005) and reactivation of opiate withdrawal memories indeed plays a role in drug seeking (Hellemans et al, 2006a, 2006b; Kenny et al, 2006). Together with the present results, slow gamma role in CS− could also underpin the drive of a dependent animal toward opiate consumption as shown for cocaine intake (Carelli and Wondolowski, 2003). Experiments of opiate self-administration would thus be of great interest to test this hypothesis.
The specific dynamics of context neurons suggests that temporal coding through gamma oscillations in NAC supports the expression of aversion and preference in morphine-dependent animals. Gamma state could provide a dynamic representation of emotional state as well as a fine index of the strength of emotional learning in opiate-dependent rats. When confronted with complex or conflicting environmental features, gamma interplay could underpin the production of behavior finely adapted to the animal’s ever-changing environment. Interestingly, in a spatial task rewarded by food, gamma oscillations in a band very similar to that of G60 have been associated with reward retrieval, whereas faster oscillations of ∼80 Hz were related to high uncertainty about the reward (van der Meer and Redish, 2009). Hence, similar electrophysiological signatures are shared by reward uncertainty and withdrawal-bound aversion (G80) but also by reward delivery and safety in the absence of withdrawal (G60). Given this presence of oscillations in the G60 and G80 bands in drug-naive animals, such mechanism could well be generalized as a physiological encoding process for emotional memories.
The aversion for the withdrawal compartment is a straight-forward and classic behavioral outcome in this associative learning paradigm (Frenois et al, 2005a). Moreover, all animals also displayed a clear preference for the compartment associated with saline injection as shown previously (Caille et al, 2003). In classical fear conditioning studies in drug-naive animals, the absence of negative outcome following a neutral stimulus is also known to lead to safety learning and specific neurophysiological responses (Genud-Gabai et al, 2013; Likhtik et al, 2014). During conditioning, physiological signs (enhanced defecation and diarrhea) were immediately correlated with the G80 signature, suggesting that aversion coding directly depends on naloxone effect. CS− pattern was different, as G60 signature developed over successive pairings. This suggests that CS− acquisition of a positive, safe emotional value is progressive. One possible explanation lies in the profound homeostatic changes observed in the limbic system of morphine-dependent subjects (Koob and Le Moal, 2008) in which neurophysiological balance relies on the presence of the drug (Dejean et al, 2013). Here, the loss of this balance upon opioid receptor blockade in CS+ pairings is repeatedly opposed to the stability experienced within CS− where a drug-dependent steady state is fueled by the continuous morphine stimulation. We therefore propose that the repetition of withdrawal absence is progressively perceived and encoded as a positive signal that confers CS− the properties of a safety context.
Our study highlights a candidate oscillatory mechanism supporting aversion and preference coding in NAC neurons. At the scale of the brain, synchronized oscillations are widely accepted as a powerful mechanism to bind distant structure activities and functions (Fell and Axmacher, 2011). NAC-projecting basolateral amygdala (BLA) and medial prefrontal cortex (mPFC) are two critical structures for associative and emotional memories (Cardinal et al, 2002) and mPFC, BLA, and NAC dynamic interactions are central in emotional memories and their expression (Ambroggi et al, 2008; Karalis et al, 2016; Klavir et al, 2013; Livneh and Paz, 2012; Popescu et al, 2007; Stuber et al, 2011). Specific oscillatory mechanisms in these structures have been shown to underpin fear (Bauer et al, 2007; Courtin et al, 2014; Dejean et al, 2016; Popa et al, 2010), appetitive conditioning (Popescu et al, 2009), and could also support discrimination of fear and safety signals (Likhtik et al, 2014). In opiate-dependent rats, mPFC, BLA, and NAC synchronize in delta, theta, and gamma frequency bands (Dejean et al, 2013) and are activated during the retrieval of opiate withdrawal memory (Frenois et al, 2005b; Lucas et al, 2012). Long-range oscillatory synchronization inside mPFC–BLA–NAC network of dependent animals could therefore support the formation and expression of emotional memories linked to opiate withdrawal. Considering the crucial role of these memories in the maintenance of drug consumption and relapse, understanding network-scale, dynamic mechanisms behind their formation and recall could lead to refined therapeutic approaches for drug addiction.
FUNDING AND DISCLOSURE
This work was supported by the CNRS, the Conseil Régional d’Aquitaine, the MILDT (Mission Interministérielle de Lutte contre la Drogue et la Toxicomanie, contract MIL0808), the ANR (Contract ANR-09-BLAN-0276), the FRM (ING20130526631 and DPA20140629799), the LABEX, and the University of Bordeaux. The authors declare no conflict of interest.
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Acknowledgements
We thank Dr Cyril Herry for critical reading of the manuscript, Pierre Gonzales for assistance during the experiments, as well as Anne Fayoux and Stéphane Lelgoualch for taking care of the animal facilities.
Author contributions
CD and PG performed the experiments. CD, MS, and AB analyzed the data. CD, CLM, SC, MC, and TB designed the experiments and contributed to the interpretation of the data. CD, MS, and CLM wrote the paper.
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Dejean, C., Sitko, M., Girardeau, P. et al. Memories of Opiate Withdrawal Emotional States Correlate with Specific Gamma Oscillations in the Nucleus Accumbens. Neuropsychopharmacol 42, 1157–1168 (2017). https://doi.org/10.1038/npp.2016.272
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DOI: https://doi.org/10.1038/npp.2016.272
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