Abstract
Copy number variants indicating loss of function in the DLG2 gene have been associated with markedly increased risk for schizophrenia, autism spectrum disorder, and intellectual disability. DLG2 encodes the postsynaptic scaffolding protein DLG2 (PSD93) that interacts with NMDA receptors, potassium channels, and cytoskeletal regulators but the net impact of these interactions on synaptic plasticity, likely underpinning cognitive impairments associated with these conditions, remains unclear. Here, hippocampal CA1 neuronal excitability and synaptic function were investigated in a novel clinically relevant heterozygous Dlg2+/− rat model using ex vivo patch-clamp electrophysiology, pharmacology, and computational modelling. Dlg2+/− rats had reduced supra-linear dendritic integration of synaptic inputs resulting in impaired associative long-term potentiation. This impairment was not caused by a change in synaptic input since NMDA receptor-mediated synaptic currents were, conversely, increased and AMPA receptor-mediated currents were unaffected. Instead, the impairment in associative long-term potentiation resulted from an increase in potassium channel function leading to a decrease in input resistance, which reduced supra-linear dendritic integration. Enhancement of dendritic excitability by blockade of potassium channels or activation of muscarinic M1 receptors with selective allosteric agonist 77-LH-28-1 reduced the threshold for dendritic integration and 77-LH-28-1 rescued the associative long-term potentiation impairment in the Dlg2+/− rats. These findings demonstrate a biological phenotype that can be reversed by compound classes used clinically, such as muscarinic M1 receptor agonists, and is therefore a potential target for therapeutic intervention.
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Introduction
Genetic variations at the DLG2 gene locus are linked to multiple psychiatric disorders including schizophrenia [1, 2], bipolar [3, 4], autism spectrum [5,6,7], attention deficit hyperactivity [8], intellectual disability [9, 10], and Parkinson’s disease [11, 12]. This clinical evidence indicates the significance of DLG2 in the aetiology of psychopathologies common to a broad range of disorders and suggests core underlying mechanisms and biological phenotypes. Many of the genetic variations are predicted to produce a loss of function for DLG2 in one copy of the gene but the resulting changes in neuronal function are poorly understood [13,14,15,16].
DLG2 is a member of a family of membrane-associated guanylate kinase (MAGUK) proteins enriched at synaptic locations that encodes the scaffolding protein PSD93 (also referred to as DLG2 or Chapsyn-110). DLG2 interacts directly with a number of other proteins in the postsynaptic density of excitatory synapses, such as NMDA receptor (NMDAR) subunit GluN2B [17,18,19,20], AMPA receptor auxiliary subunit stargazin [21], potassium channels Kir2.3 [22], Kir2.2 [23] and Kv1.4 [24], as well as proteins involved in potassium channel palmitoylation, cell adhesion, microtubule assembly, and cell signalling, palmitoyltransferase ZDHHC14 [25], neuroligin1-3 [17], Fyn [26, 27], ERK2 [28], GKAP [29], and MAP1A [30]. Uniquely to the MAGUK family, DLG2 is targeted to the axon initial segment where it regulates neuronal excitability via its interactions with potassium channels [25, 31]. At a functional level, homozygous Dlg2−/− knockout mice have altered glutamatergic synapse function [32,33,34,35] and impaired long-term potentiation (LTP) in the hippocampus [33]. These synaptic perturbations could underlie the common cognitive psychopathologies of the psychiatric disorders associated with DLG2. Indeed, Dlg2−/− mice have been shown to have impaired performance in the object-location paired associates learning task [36]. Homozygous Dlg2−/− mice also exhibit increased grooming behaviour [35] and altered social interaction but without consistent effects in negative valence tasks such as the open field test [35, 37].
Impaired synaptic plasticity resulting from the loss of DLG2 is a potential biological phenotype underpinning trans-diagnostic cognitive psychopathologies but the mechanism by which reduced DLG2 expression leads to impaired synaptic plasticity is unclear. Furthermore, mechanistic understanding for the impact of DLG2 loss may reveal new targets for therapeutic intervention. Most animal models for DLG2 loss have employed full knockouts of the gene but these do not accurately represent the heterozygous nature of DLG2 genetic variants in patient populations and potentially engage compensatory expression by other MAGUK proteins [32, 38] that is not present in heterozygous reduced gene dosage models (Supplementary Fig. S3). Therefore, here we investigate the combined impact of low gene dosage DLG2 on synaptic function, neuronal excitability and morphology using a novel CRISPR-Cas9 engineered heterozygous Dlg2+/− (het) rat model to understand the interactions that lead to impaired synaptic plasticity and cognitive function.
Materials and methods
Animals and husbandry
All procedures were carried out under local institutional guidelines, approved by the University of Bristol Animal Welfare and Ethical Review Board, and in accordance with the UK Animals (Scientific procedures) Act 1986. The experiments employed a novel Dlg2+/− heterozygous rat model generated on a Hooded Long Evans background using CRISPR-Cas9 genomic engineering that targeted a 7 bp deletion to exon 5 of the rat Dlg2 gene, resulting in a downstream frame shift in exon 6 and the production of a premature stop codon that led to a reduction in Dlg2 protein levels in the hippocampus (Supplementary Figs. S1–3). Full details of the creation, quality control and off-target assessment of the Dlg2+/− model can be found in Supplementary information. Male Dlg2+/− rats were bred with wild type (wt) female rats, generating mixed litters of Dlg2+/− and wt littermate offspring. The Dlg2+/− animals were viable and showed no signs of ill health, with normal litter sizes containing the expected Mendelian ratio of positive to wt genotypes and normal sex ratios, there were no effects in survival of the Dlg2+/− rats to adulthood and no effects on general morbidity, including fertility, or mortality throughout the lifespan. Further details of animal husbandry, breeding strategy and viability are described in Supplementary information. Approximately equal numbers of each sex rats aged P50-75 were used, with experimenter blind to genotype during experiments and data analysis.
Methods on brain slice preparation, electrophysiology, protein quantification and computational modelling are in the supplementary information.
Statistical analysis
3-way and 2-way ANOVA, 3-way repeated measures ANOVA, Komolgorov–Smirnov test, as well as paired and unpaired t-tests were used as appropriate, with full statistical results available in Supplement 2. Genotype, sex, and dorsal-ventral aspects of the hippocampus, as well as repeated measurements, were factored into all analyses, as appropriate. Genotype was viewed as the primary output factor shown in the figures. No genotype-sex and only one genotype-aspect interactions were found indicating limited impact of sex and aspect on the primary genotype factor results but where effects of other factors were found, the data are presented in Supplement 1 (Supplementary Figs. S8–16, S18 and 19). Inclusion of animals in the analysis of a subset of experiments using multi-level general linear mixed modelling did not affect the statistical results indicating that the major source of variability arose between cells rather than animals. Therefore, cell was defined as the experimental unit and we report numbers of cells and animals in figure legends. α = 0.05 was applied for all tests, except the Kolmogorov–Smirnov test where α = 0.01 was applied. The degrees of freedom, F, and P values are presented in the text, figures, and Supplement 2.
Results
Dlg2+/− heterozygous knockout rats were generated by CRISPR-Cas9 targeting of the Dlg2 gene (Supplementary information). In this model, DLG2 protein expression levels were reduced by ~50% in hippocampus without effects on expression of other components of the postsynaptic density, including the closely related MAGUK DLG4 (PSD95) and the GluN1 NMDAR subunit (Supplementary Fig. S3). The specific reduction in DLG2 protein expression was replicated in tissue from prefrontal cortex, posterior cortex and cerebellum and mirrored by a ~50% reduction of dlg2 mRNA expression, without any change in dlg1, dlg3 or dlg4 mRNA [39].
Associative LTP
The learning of novel representations in CA1 is thought to arise from the dendritic integration of spatiotemporally coherent inputs from the entorhinal cortex (via the temporoammonic (TA) pathway) and CA3 (via the Schaffer collateral (SC) pathway) that can summate supra-linearly to drive associative LTP (aLTP) [40,41,42,43,44,45,46,47]. LTP in CA1 of hippocampus is impaired in homozygous Dlg2−/− mice [33] but the interpretation of these results is complicated by the potential for compensation by other MAGUK proteins [32, 38]. The heterozygous Dlg2+/− rat offers the opportunity to test whether LTP is impaired in the absence of any MAGUK compensation.
aLTP was assessed in the CA1 region of hippocampal slices by stimulating the SC and TA pathways simultaneously with a theta burst stimulation pattern whilst recording from CA1 pyramidal neurons (Supplementary Fig. 1A, B). An additional independent SC pathway was also stimulated as a negative control and a pathway check was done to confirm pathway independence (Supplementary Fig. S4). The induction protocol resulted in robust aLTP in the wts but reduced aLTP in the Dlg2+/− hets in both SC and TA test pathways (Fig. 1C–E). During induction the number of elicited action potential bursts and single spikes was reduced in the Dlg2+/− hets, despite baseline EPSC amplitudes being unchanged indicating that all neurons received similar inputs, but there was a trend suggesting reduced overall depolarisation in response to synaptic stimulation (Fig. 1F–J). Both spike number and depolarisation during induction correlated with LTP in the SC pathway but not the TA pathway (Supplementary Fig. S5). There was no effect of genotype on the after-hyperpolarisation (Fig. 1K). This suggests that in the Dlg2+/− hets, the integration of synaptic inputs from the SC and TA pathways is impaired, reducing dendritic depolarisation and action potential spiking which are the drivers of aLTP.
To test the necessity of action potentials for aLTP, a paired theta burst LTP induction protocol was used, where action potentials were driven by somatic current injection to bypass dendritic integration, and spikes were paired with simultaneous SC pathway stimulation (Fig. 1L). Under these conditions, robust LTP was induced in the SC pathway in both genotypes, with the TA pathway acting as negative control (Fig. 1M–O). Similarly, when aLTP was tested using baseline EPCSs doubled in amplitude, maximal LTP was induced and there was no effect of genotype (Supplementary Figs. S6 and 7). As expected, aLTP was greater in ventral slices whereas theta burst LTP was greater in dorsal slices (Supplementary Figs. S8 and 9). This indicates that the hets are fundamentally able to undergo LTP but their ability to integrate inputs is impaired.
Synaptic integration
To directly test synaptic integration, the number of activated synapses required to generate supra-linear summation of EPSPs across multiple dendrites was assessed, which is a measure of the ability for synapses to integrate across the dendritic arbor [40, 42, 47] and allowed comparison of synaptic integration between genotypes. To activate increasing numbers of synapses, the SC pathway was stimulated with increasing intensity to activate synapses with a random spatial distribution across the proximal and basal dendritic arbor. The number of activated synapses was measured by the slope of a single EPSP, and the integration of synapses assessed by the amplitude and duration of a compound summated EPSP (area under the curve – AUC) elicited by repetitive high frequency synaptic stimulation (Fig. 2A). As stimulation intensity was increased the number of active synapses increased in a linear relationship with the amplitude and durations of the summated compound EPSP until a “change point” was reached (see methods) after which the relationship became supra-linear because the duration of the compound EPSP increased (Fig. 2B), indicative of activation of regenerative or plateau potentials within the dendrites [40, 42, 47]. The inhibition of these regenerative potentials by D-APV demonstrated their dependence on NMDAR activation (Fig. 2A, B). The change point was increased in the Dlg2+/− hets (Fig. 2B, C), indicating that het neurons required more synaptic inputs to undergo the transition to supra-linear integration. Additionally, the maximum duration of the compound EPSP as a ratio to the corresponding slope of the single EPSP was reduced in the Dlg2+/− hets (Fig. 2D). This again indicates that Dlg2+/− hets require more synaptic input to integrate dendritic inputs and produce the supra-linear regenerative potentials important for aLTP.
NMDAR currents
Synaptic integration is driven by NMDARs and protein-protein interaction studies have reported DLG2 to interact directly with NMDAR subunits [17,18,19] and with AMPAR indirectly [21]. Further, DLG2 has also been shown to affect glutamatergic function in homozygous Dlg2−/− models, albeit with variable results in AMPA/NMDA ratio [32,33,34, 48, 49]. To investigate whether glutamatergic function was affected in Dlg2+/− rats and whether this might explain the impairment in synaptic integration and aLTP, the AMPA/NMDA ratio was measured in CA1 pyramidal neurons (Fig. 3A). Dlg2+/− hets had a reduced AMPA/NMDA ratio in the SC pathway, with no effect in the TA pathway (Fig. 3B–D). AMPAR-mediated miniature excitatory postsynaptic currents (mEPSCs, Supplementary Fig. S10) resulting from the activity of single synapses were recorded to probe whether the reduction in AMPA/NMDA ratio resulted from a reduction in AMPA, an increase in NMDA, or a combination of the two. The slow kinetics and small amplitude of NMDAR-mediated mEPSCs make them difficult to detect accurately. AMPAR-mediated mEPSCs from synapses on proximal dendrites are more detectable than those from more distal synapses due to signal attenuation and therefore recorded mEPSCs will arise from the most proximal synapses [50]. There was no difference in the distributions of mEPSC amplitude, interevent interval, or decay tau across genotype (Fig. 3E–I). Paired-pulse facilitation, measured in the AMPA/NMDA ratio experiment, was also not different across genotype in either pathway (Supplementary Fig. S11). Together, these results show no change in postsynaptic AMPAR function and presynaptic glutamate release probability in the SC pathway. It follows that the AMPA/NMDA ratio effect in the SC pathway was due to an increase in NMDAR function. This could result from either an increase in NMDAR number or a change in subunit composition between GluN2A and GluN2B. To test subunit composition, NMDAR currents were isolated (Fig. 3J) and the selective GluN2B negative allosteric modulator RO256981 was applied. RO256981 decreased EPSC amplitude (Fig. 3J–L) and increased the EPSC decay time in both the SC and the TA pathways (Fig. 3M, N). There was a trend toward a genotype x drug interaction in the EPSC amplitude measurement in the SC pathway but no genotype x drug interaction in the EPSC decay kinetics. Together, these results show similar NMDAR subunit composition across genotype and therefore the enhancement in synaptic NMDAR function likely arises from increased receptor numbers at SC synapses, despite overall neuronal receptor expression remaining constant. Enhanced synaptic NMDAR function is expected to increase synaptic integration and therefore cannot explain the observed decrease in integration.
Input resistance
Reduced synaptic integration in dendrites could arise from multiple mechanisms. Based on previous findings in CA1 pyramidal neurons the three most likely are: i) Reduced expression of hyperpolarisation-activated cyclic nucleotide-gated (HCN) channels that regulate neuronal excitability and contribute to dendritic integration [51,52,53], ii) Increased expression of small conductance calcium-activated potassium (SK) channels that inhibit NMDARs at synapses, reducing dendritic integration and LTP [54,55,56], iii) Reduced input resistance by increased potassium channel expression particularly in dendritic locations to reduce dendritic integration and LTP [42, 47, 57,58,59,60,61]. Each of these mechanisms was directly tested.
Pharmacological blockade of HCN channels with ZD7288 produced robust effects on neuronal excitability (including spiking, sag, and input resistance) but there were no differential effects across genotype (Supplementary Fig. S12). There were also no genotype-specific effects on cellular resonance or impedance that are directly dependent on HCN channels [62,63,64,65,66] (Supplementary Fig. S13) with some effects of sex and aspect (Supplementary Figs. S14,15,17,18). As previously described, the SK channel blocker apamin produced an increase in EPSP duration in the SC and TA pathways (Supplementary Fig. S12) indicating increased NMDAR activation during synaptic stimulation [54,55,56]. However, the regulation of synaptic NMDAR function by SK channels was similar between genotypes indicating no change in SK channel expression. Therefore, differential HCN or SK channel function is unlikely to explain the difference in synaptic integration between genotypes.
To assess input resistance, measurements were analysed from voltage clamp experiments (using identical conditions to the LTP experiments in Fig. 1) and in current clamp experiments. In both these separate and independent data sets Dlg2+/− hets had reduced input resistance (Fig. 4A–D). This increase in electrical leak in the Dlg2+/− hets is predicted to reduce cross-talk between synapses and their integration leading to a reduced spike output but it is also expected to reduce the spike output in response to somatic current injection. However, despite reduced input resistance in the Dlg2+/− hets, there was no effect of genotype on spike output to current injection (rheobase) (Fig. 4E, F). This could be explained by a depolarised resting membrane potential (Fig. 4G) and a trend towards hyperpolarised action potential spike threshold (Fig. 4H) in the Dlg2+/− hets indicating that smaller membrane potential depolarisations were required to initiate spikes. There was no effect of genotype on spike half-width, maximum spike slope, spike amplitude, or capacitance, and a slight decrease in latency to spike in the Dlg2+/− hets (Supplementary Fig. S16).
Reduced input resistance in the Dlg2+/− hets could be explained via two mechanisms: i) increased membrane area through greater dendritic branching and extent [42, 67] or ii) increased membrane conductance, most likely caused by increased potassium channel expression. To test the first mechanism, a subset of neurons from the intrinsic excitability experiments were filled with neurobiotin to allow post hoc morphological analysis. Analysis of these neurons revealed that het neurons were smaller than wt neurons (Fig. 4I) and had reduced dendritic branch number and total dendritic branch length but had similar mean dendritic branch lengths (Fig. 4J–L). Scholl analysis demonstrated that Dlg2+/− het neurons had reduced dendritic arborisation overall, with the most striking differences in the basal and proximal apical regions (Fig. 4M). Contrary to the predicted neuronal size – input resistance relationship, there was no correlation between total dendritic branch length and input resistance (Fig. 4N). Therefore, reduced neuronal arborisation in the Dlg2+/− hets cannot explain the observed reduced input resistance and instead increased potassium channel expression is the most likely explanation.
Computational modelling of synaptic integration in representative reconstructed pyramidal neurons also predicted increased potassium channel expression as the mechanism underlying reduced input resistance (Supplementary Fig. S19) and enabled exploration of the likely potassium channel subtypes mediating reduced synaptic integration. DLG2 interacts with potassium inward rectifier Kir2.3 [22] and Kir2.2 [23] as well as A-type Kv1.4 [24] channels which therefore represent potential candidates to underpin decreased input resistance and synaptic integration. The model suggested that A-type potassium channels are the most likely candidates upregulated in the Dlg2+/− hets to underly the dendritic integration deficits (Supplementary Fig. S19). However, any mechanism that reduces input resistance is predicted to facilitate dendritic integration in the Dlg2+/− hets.
Rescue of synaptic integration and plasticity
The aLTP, theta burst LTP, and dendritic integration results from Figs. 1 and 2 suggest that, given enough synaptic input, Dlg2+/− hets can express LTP despite their reduced input resistance. It follows that by increasing input resistance in the Dlg2+/− hets, dendritic integration and aLTP could be effectively rescued. Three separate methods to increase input resistance were tested for their effectiveness in rescuing dendritic integration. The first was the relatively broad-spectrum voltage-sensitive potassium channel blocker, 4-aminopyridine (4-AP) [68], the second was the selective Kv1.3, Kv1.4 blocker CP339818 [69] and the third was activation of muscarinic M1 receptors [54, 70]. 4-AP caused an increase in input resistance, a reduction in the supra-linearity change point, a trend toward increased maximum duration of the compound EPSP as a ratio to the corresponding slope of the single EPSP, and a repolarisation in resting membrane potential (Fig. 5A–E). The effects of 4-AP were not genotype-specific, as there were no drug x genotype interactions. These results support the computational modelling predictions that voltage-sensitive potassium channels attenuate dendritic integration and blocking them facilitates it. Since DLG2 interacts with Kv1.4, the selective blocker CP339818 was used to test whether the upregulation of these channels was responsible for reduced dendritic integration. However, CP339818 had no effect on input resistance or dendritic integration (Supplementary Fig. S20) indicating that upregulation of these specific A-type potassium channels does not underpin the reduction in dendritic integration in the Dlg2+/− hets but does not rule out a role for other A-type channels.
These results demonstrate, as predicted, that blocking a subset of potassium channels activated around the resting membrane potential facilitates dendritic integration. However, due to the considerable heterogeneity of potassium channels and their ability to compensate for one another coupled with limited availability of selective pharmacological tools, identifying and targeting the precise channels that cause reduced input resistance in the Dlg2+/− hets is challenging. An alternative approach, and one with greater therapeutic potential, is to rescue the input resistance reduction indirectly, for example by activation of cholinergic muscarinic M1 receptors that inhibit potassium channel function and increase dendritic excitability [42, 47, 57,58,59,60,61]. Support for this approach was found using the highly selective muscarinic M1 receptor allosteric partial agonist 77-LH-28-1 [71] which increased input resistance, reduced the change point, increased the maximum duration of the compound EPSP as a ratio to the corresponding slope of the single EPSP, and depolarised the resting membrane potential (Fig. 5F–J). However, there were no significant drug x genotype interactions. Similar results were found for the broad-spectrum non-hydrolysable acetylcholine analogue carbachol (Supplementary Fig. S21).
These results suggest that pharmacological enhancement of dendritic excitability and integration may be sufficient to rescue aLTP in the Dlg2+/− hets. Therefore, the aLTP experiment was repeated in the presence of 77-LH-28-1. This rescued aLTP in the Dlg2+/− hets with robust aLTP in SC and TA pathways (Fig. 5K–M). In addition, unlike in the absence of 77-LH-28-1, there was no effect of genotype and no pathway x genotype interaction (Fig. 5M), indicating 77-LH-28-1 selectively rescues aLTP in the Dlg2+/− hets. Importantly, baseline EPSC amplitude did not differ among pathways and across genotype (Fig. 5N), indicating that the amount of synaptic input received was similar in all conditions. Analysis of the aLTP induction phase revealed that 77-LH-28-1 rescued synaptic summation and the resulting action potential spiking (Fig. 5O–R) as well as plateau potential generation (Supplementary Fig. S22), with the genotypic differences for number of bursts, EPSP summation, and spike number disappearing. Taken together, Fig. 5 shows 77-LH-28-1 reduced the threshold for dendritic integration in both wts and Dlg2+/− hets but selectively facilitated aLTP in the Dlg2+/− hets indicating induction of synaptic plasticity in the Dlg2+/− hets is more sensitive to increased dendritic excitability and synaptic integration.
Discussion
NMDAR currents are increased in the Dlg2+/− heterozygous rat model. Additionally, dendritic arborisation is reduced. These observations would be expected to combine to enhance neuronal excitability, dendritic integration and synaptic plasticity. Instead, the effects are entirely offset, and indeed reversed, by a concomitant reduction in input resistance caused by an increase in potassium channel expression, potentially A-type potassium channels. This increase in electrical leak is the dominant effect, resulting in a final phenotype where dendritic integration and aLTP are impaired. Crucially, dendritic integration can be rescued by potassium channel block or activation of muscarinic M1 receptors, the latter of which can also rescue synaptic plasticity. These phenotypes are potentially particularly relevant since the Dlg2+/− rat model relates to human single copy genetic variants.
The direct interaction between DLG2 and GluN2b NMDAR subunits suggest the most important effects of DLG2 perturbations are on NMDAR function – synaptic integration and plasticity. However, previous studies on Dlg2−/− full knockout models have either reported no changes in the AMPA/NMDA ratio or a reduction in the AMPA/NMDA ratio due to reduction in AMPAR function [32,33,34, 48, 49], Here, AMPAR function was unchanged and instead we found an unexpected increase in NMDAR currents, likely caused by increased synaptic expression selectively at Schaffer collateral synapses. There is no evidence that DLG2 is differentially expressed at Schaffer collateral vs temporoammonic synapses in CA1 so the mechanism for this selective enhancement of NMDAR expression is unknown. On its own, enhanced NMDAR currents predict enhanced aLTP, but we found the converse with aLTP impairment. This is similar to previous reports in Dlg2−/− mice. In homozygous Dlg2−/− mice CA1 LTP was normal in response to strong 100 Hz induction protocol but reduced in response to TBS given to just the SC pathway [33]. In our study using heterozygous Dlg2+/− rats, TBS-induced LTP pairing postsynaptic stimulation with SC input was normal and an LTP deficit only became apparent in the Dlg2+/− model when neurons were required to integrate converging inputs suggesting a nuanced and potentially behaviourally relevant phenotype in the clinically relevant Dlg2+/− model. Furthermore, synaptic integration and the initiation of non-linear dendritic events are key determinants of feature detection and selectivity in neuronal networks [41, 72, 73] and a deficit in detecting events and giving appropriate salience are important features of many psychiatric disorders [74].
The dichotomy between enhanced NMDA currents and reduced NMDAR function in Dlg2+/− rats during aLTP highlights the dominant role played by changes to intrinsic neuronal excitability; in this instance reduced input resistance caused by increases in potassium channel function. Interestingly, in a Dlg2−/− full knockout model no changes in input resistance were reported [75] highlighting again the importance of using clinically relevant models. In our Dlg2+/− model this increase in potassium channel function does not appear to be caused by a direct interaction with DLG2 but instead as a homoeostatic regulatory mechanism perhaps to compensate for increased synaptic currents. A similar compensatory mechanism is found in other models of psychiatric disorders such as Fmr1-/y mice where changes in intrinsic neuronal excitability dominate the resulting perturbations in network processing including dendritic integration and synaptic plasticity [63, 76,77,78]. This raises the intriguing possibility that genetic disruptions to synaptic function may generally cause homoeostatic compensations in intrinsic neuronal excitability that dominate neuronal function and present a common biological phenotype across multiple psychiatric disorders [79].
We have demonstrated in this study that the compensatory mechanisms affecting neuronal excitability can be ameliorated pharmacologically with the administration of selective agonists such as 77-LH-28-1 rescuing impairments in synaptic integration and plasticity, a proof of principle that may be applicable to other psychiatric disorder risk variants. For example, an increase in input resistance due to the administration of 77-LH-28-1 could facilitate spike backpropagation, potentially rescuing the plasticity impairment and network dysfunctions reported in the Cacna1c+/− and 22q11 deletion syndrome models of genetic vulnerability to schizophrenia [80, 78], Highly selective muscarinic M1 receptor agonists have efficacy clinically with negligible side effects [81,82,83,84] making them attractive pharmaceutical tools. It remains to be seen whether behavioural impairments in DLG2 models can be rescued using similar pharmacological strategies.
Change history
15 January 2024
A Correction to this paper has been published: https://doi.org/10.1038/s41386-024-01801-w
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Acknowledgements
We thank Jenny Carter for coordinating the initial generation and breeding of the Dlg2+/− rat line, Rachel Humphries for computational modelling discussions and Aleks Domanski and all members of the Robinson and Mellor groups for general discussions. We also thank Hannah Jones and Estela Michail for their input in the study of morphology.
Funding
The authors gratefully acknowledge funding from Medical Research Council (UK) (CO’D, GW4 BIOMED PhD studentship to SG), Biotechnology and Biological Science Research Council (UK) (JRM), Wellcome Trust (UK)101029/Z/13/Z (JRM, PhD studentship to SW). The Dlg2+/− rats were generated as part of a Wellcome Trust Strategic Award ‘DEFINE’ (JH and LSW) and the Wellcome Trust Strategic Award and the Neuroscience and Mental Health Research Institute, Cardiff University, UK provided core support. ER has received research funding from Boehringer Ingelheim, Eli Lilly, Pfizer, Small Pharma Ltd. and MSD, and DD has received research funding from Eli Lilly, but these companies were not associated with the data presented in this manuscript.
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Conceptualisation, SG, JH, LSW, ESJR, JRM.; Methodology, SG, CO’D, JRM; Investigation and Analysis, SG, SW; Writing, SG, SW, CO’D, KLT, DMD, LSW, JH, ESJR, JRM; Supervision, KLT, DWD, LSW, JH, ESJR, JRM.
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Griesius, S., O’Donnell, C., Waldron, S. et al. Reduced expression of the psychiatric risk gene DLG2 (PSD93) impairs hippocampal synaptic integration and plasticity. Neuropsychopharmacol. 47, 1367–1378 (2022). https://doi.org/10.1038/s41386-022-01277-6
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DOI: https://doi.org/10.1038/s41386-022-01277-6
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