Abstract
TNF receptor-associated factor 6 (TRAF6)-BECN1 signaling axis plays a pivotal role in autophagy induction through ubiquitination of BECN1, thereby inducing lung cancer migration and invasion in response to toll-like receptor 4 (TLR4) stimulation. Herein, we provide novel molecular and cellular mechanisms involved in the negative effect of ubiquitin-specific peptidase 15 (USP15) on lung cancer progression. Clinical data of the TCGA and primary non-small cell lung cancer (NSCLC) patients (n = 41) revealed that the expression of USP15 was significantly downregulated in lung cancer patients. Importantly, USP15-knockout (USP15KO) A549 and USP15KO H1299 lung cancer cells generated with CRISPR-Cas9 gene-editing technology showed increases in cancer migration and invasion with enhanced autophagy induction in response to TLR4 stimulation. In addition, biochemical studies revealed that USP15 interacted with BECN1, but not with TRAF6, and induced deubiquitination of BECN1, thereby attenuating autophagy induction. Notably, in primary NSCLC patients (n = 4) with low expression of USP15, 10 genes (CCNE1, MMP9, SFN, UBE2C, CCR2, FAM83A, ETV4, MYO7A, MMP11, and GSDMB) known to promote lung cancer progression were significantly upregulated, whereas 10 tumor suppressor genes (FMO2, ZBTB16, FCN3, TCF21, SFTPA1B, HPGD, SOSTDC1, TMEM100, GDF10, and WIF1) were downregulated, providing clinical relevance of the functional role of USP15 in lung cancer progression. Taken together, our data demonstrate that USP15 can negatively regulate the TRAF6-BECN1 signaling axis for autophagy induction. Thus, USP15 is implicated in lung cancer progression.
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Introduction
Autophagy is a functional response to a variety of cellular conditions, such as genomic damage, metabolic stress, infections, and tumorigenesis [1,2,3,4,5,6]. It has been reported that toll-like receptor (TLR)-mediated signaling plays a pivotal role in cancer progression through autophagy induction and the production of cytokines such as interleukin-6 (IL-6), chemokine (C-C motif) ligand 2 (CCL2/MCP-1), chemokine (C-C motif) ligand 20 (CCL20/MIP-3α), vascular endothelial growth factor A (VEGFA), and matrix metallopeptidase 2 (MMP2) [7]. In terms of molecular mechanisms, these cellular events are critically dependent on TNF receptor-associated factor 6 (TRAF6) signaling [7]. TRAF6, an E3 ubiquitin-protein ligase and adaptor protein, can functionally regulate downstream signaling cascades for the production of cytokines through the activation of the nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB) and the induction of autophagy through ubiquitination of beclin 1 (BECN1) [7,8,9,10,11,12]. Recent studies have demonstrated that interruption of TRAF6-BECN1 signaling induced by TLR4 can lead to inhibition of autophagy induction, resulting in attenuated cancer migration and invasion [10,11,12]. This suggests that the TRAF6-BECN1 signaling axis plays a pivotal role in cancer progression through autophagy induction.
Deubiquitinating enzymes (DUBs) can counteract cellular E3 ubiquitin ligases by removing ubiquitin from substrates, thereby regulating a variety of cellular signaling processes [13]. Ubiquitin-specific peptidase 15 (USP15) is a member of the largest subfamily of cysteine protease DUBs [14, 15]. We have previously reported that charged multivesicular body protein 5 (CHMP5) can cooperate with a PDB genetic risk factor valosin-containing protein (VCP/p97) to stabilize the inhibitor of NF-κB, thus downregulating ubiquitination of IκBα via the deubiquitinating enzyme USP15 [16]. Furthermore, USP15 can antagonize parkin-mediated mitochondrial ubiquitination and mitophagy [17]. Knockdown of USP15 can rescue the mitophagy defect in fibroblasts of patients with Parkinson’s disease (PD) and increase their parkin levels [17]. Although much research progress has been made in exploring the roles of DUBs in various human diseases [13], we know little about the molecular and cellular mechanisms by which USPs are implicated in cancer progression. Thus, this study explores the molecular and cellular mechanism by which USP15 is implicated in cancer progression through the regulation of the TRAF6-BECN1 signaling axis for autophagy induction.
TCGA data analysis revealed that five different cancer types (LUAD, LUSC, OV, SKCM, and TGCT) showed significant downregulation of USP15, whereas no significant difference in expression of USP15 was seen in the other 28 different cancer types. Notably, the downregulation of USP15 was significant in primary non-small cell lung cancer (NSCLC) patients, especially in patients with lung adenocarcinoma. Based on these findings and previous reports [16, 17], we investigated whether USP15 as a deubiquitinating enzyme was implicated in lung cancer progression, especially through the regulation of TRAF6-BECN1 signaling axis for autophagy induction. We found that USP15 interacted with BECN1, but not with TRAF6, and induced deubiquitination of BECN1. Importantly, USP15-knockout (USP15KO) A549 and USP15KO H1299 lung cancer cells generated by CRISPR-Cas9 gene editing showed increases of cancer migration and invasion accompanied by enhanced autophagy induction in response to TLR4 stimulation. These results strongly support that USP15 is negatively implicated in lung cancer progression through the regulation of autophagy induction. Regarding clinical aspects, primary LUAD patients (n = 4) with low expression of USP15 showed significant upregulation of genes (n = 10) involved in the promotion of lung cancer proliferation, migration, invasion, and metastasis, whereas tumor suppressor genes (n = 10) were downregulated. These results strongly suggest that USP15 is negatively implicated in lung cancer progression through the TRAF6-BECN1 signaling axis by regulating autophagy induction.
Materials and methods
Patient-derived non-small cell lung cancer samples (NSCLC)
Tumor and matched normal tissues were obtained from 41 patients with primary NSCLC in accordance with the ethical principles stated in the Declaration of Helsinki. This study was approved by the Institutional Review Board (IRB) of Samsung Medical Center (SMC) (IRB#: 2010-07-204), following procedures previously described [18, 19]. We obtained written informed consent from each patient before surgery for using their pathological specimens for research use.
Cells
Human embryonic kidney (HEK) 293T cells (ATCC, CRL-11268) were cultured and maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, 11965092) with 10% fetal bovine serum (FBS). A549 cells (human lung cancer cell line; ATCC, CCL-185) and H1299 cells (human non-small cell lung cancer cell line; ATCC, CRL-5803) were maintained in RPMI 1640 medium (Sigma-Aldrich, 31800-022) supplemented with 10% FBS, penicillin (100 μg/mL), and streptomycin (100 μg/mL) in a 5% CO2 humidified atmosphere at 37 °C.
Antibodies and reagents
Anti-MYC (2276), anti-GAPDH (2118), and anti-LC3A/B (4108) were purchased from Cell Signaling Technology. Anti-FLAG (SAB4200071) was purchased from Sigma-Aldrich. Anti-HA (ab18181) and anti-USP15 (ab97533) were purchased from Abcam. Lipopolysaccharide (LPS; serotype 0128: B12), chloroquine (CQ; C6628), dimethyl sulfoxide (DMSO; 472301), puromycin (P8833), paraformaldehyde (P6148), Triton X-100 (T8787), 3-methyladenine (3-MA; M9281), gentamicin (G1272), deoxycholate (D6750), and Dulbecco’s phosphate-buffered saline (DPBS; D8537) were purchased from Sigma-Aldrich. Lipofectamine 2000 (11668019) was purchased from Thermo Fisher Scientific.
Plasmid constructs
FLAG-TRAF6 (21624), FLAG-HA-USP15 (22570), and FLAG-BECN1 (24388) plasmids were purchased from Addgene. HA-tagged Ub plasmids were obtained from Dr. J. H. Shim (University of Massachusetts Medical School, USA). Using the FLAG-HA-USP15 plasmid, full-length FLAG-USP15 and MYC-USP15 constructs were cloned into a pCMV-3Tag-7 (Agilent technologies, 240202) or a pCMV-3Tag 6 vector (Agilent technologies, 240200). Using FLAG-BECN1, the full-length MYC-BECN1 construct was cloned into a pCMV-3Tag-7 vector (Agilent Technologies, 240202). Truncated mutants of MYC-BECN1 were generated as previously described [10, 20,21,22,23]. MYC-USP15 C269A and MYC-USP15 H862A mutants were generated by site-directed mutagenesis as previously described [24].
Generation of USP15-knockout cancer cell lines by CRISPR/Cas9
Guide RNA sequences for CRISPR/Cas9 were designed as 5’-CACCG CAGTTGGGACAAATACCAGATGG-3’ and 3’-CGTCAACCCTGTTTATGGTCTACC CAAA-5’ for human USP15. USP15KO A549 and USP15KO H1299 cancer cells were generated as previously described [11, 12, 25]. Complementary oligonucleotides to guide RNAs (gRNAs) were annealed and cloned into a lentivirus CRISPR v2 vector (Addgene plasmid, 52961). Lenti CRISPR v2/gRNA was transfected into A549 or H1299 cells using Lipofectamine 2000 according to the manufacturer’s instructions. USP15KO A549 and USP15KO H1299 cell colonies were selected as previously described [11, 12, 25] and confirmed by western blots.
Western blotting (WB) and immunoprecipitation (IP) assays
WB and IP assays were performed as previously described [10, 20,21,22,23]. Briefly, HEK 293T cells were seeded into six-well plates, transfected, and treated as described in the text and Figures. These cells were then incubated for 38–48 h. After collecting cells, cell lysates were prepared and immunoprecipitated with anti-MYC and anti-FLAG antibodies. IP complexes were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, 6–10%) and immune-probed with different antibodies as indicated in the text. For ubiquitination assay, mock vector, FLAG-tagged BECN1, MYC-tagged USP15 wild-type (WT), MYC-tagged USP15 C269A mutant, and MYC-tagged USP15 H862A mutant were transfected separately into HEK 293T cells along with HA-tagged Ub as described in the text and Figures. Cell lysates were immunoprecipitated with anti-MYC, anti-HA, or anti-FLAG antibodies and probed with different antibodies as indicated in the text. Control (Ctrl) A549 and USP15KO A549 cells were treated with or without the vehicle or CQ (10 μM) or 3-MA (5 mM) in the presence or absence of LPS (10 μg/mL) for 6 h. Cell lysates were immunoblotted with an anti-LC3A/B antibody and anti-GAPDH (as a loading control). For a semi-endogenous IP assay, A549 and H1299 lung cancer cells were transiently transfected with FLAG-USP15. At 48 h post-transfection, cells were treated with or without LPS (10 μg/mL) for 60 min. After collecting cells, cell lysates were prepared and immunoprecipitated with anti-Ig or anti-FLAG antibodies. IP complexes were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, 6–10%) and immune-probed with anti-FLAG and anti-BECN1 antibodies.
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis
Control (Ctrl) A549, USP15KO A549, Ctrl H1299, and USP15KO H1299 cells were treated with or without 10 μg/mL LPS for 6 h. RT-qPCR analysis was performed as previously described [11]. Briefly, after extracting total RNA using an RNA isolation kit (A&A Biotechnology, Gdynia, Poland) according to the manufacturer’s protocol, cDNA was prepared by RT using AmfiRivert II cDNA Synthesis Master Mix (genDEPOT, R550) according to the manufacturer’s protocol. Primers hIL-6 (PPH 00560C), hMMP2 (PPH 00151B), hCCL20 (PPH 00564C-200), and hCCL2 (PPH 00192F) were purchased from Qiagen, Inc. (Chatsworth, CA, USA). Fluorescence detection was performed using an ABI PRISM 7700 Sequence Detector (PerkinElmer; Applied Biosystems; Thermo Fisher Scientific, Inc.). The mRNA expression level was calculated and normalized to the level of GAPDH.
Wound-healing migration assay
A wound-healing migration assay was performed following previous protocols [10,11,12]. Briefly, Control (Ctrl) A549, USP15KO A549, Ctrl H1299, and USP15KO H1299 cells were seeded into 12-well plates and cultured to reach confluence. Cell monolayers were gently scratched and washed with culture medium. After floating cells and debris were removed, cells attached to culture plates were treated with vehicle (DMSO), 3-MA (5 mM), or CQ (10 μM) in the presence or absence of LPS (10 μg/mL). Cell images were captured after culturing for different periods as indicated in each experiment.
Transwell invasion assay
Transwell invasion assay was performed following previous protocols [10,11,12]. Briefly, Control (Ctrl) A549, USP15KO A549, Ctrl H1299, and USP15KO H1299 cells were suspended in culture medium (200 μL) without FBS. Cells were then added to the upper compartment of a 24-well Transwell® chamber containing a polycarbonate filter with 8-mm pores and coated with 60 mL of Matrigel (Sigma-Aldrich, E1270; 1:9 dilution). Culture medium with 10% FBS was added to the lower chamber. After incubating for 24 h, cells in the upper compartment were removed, washed with PBS, and fixed. Invaded cells were stained with 4,6-diamidino-2-phenylindole (Sigma-Aldrich, D9542) and quantified by counting the number of fluorescent cells.
Microarray analysis
Microarray analysis was performed as previously described [18, 19]. From tumor and matched normal tissues of 41 patients with NSCLC, total RNAs were extracted with Trizol (Thermo Fisher Scientific, 15596026) and purified using RNeasy columns (Qiagen, 74106) according to each manufacturer’s protocol. Microarray analysis was performed and analyzed as previously described [18, 25,26,27].
TCGA data analysis
The expression of USP15 in human lung cancers (lung adenocarcinoma, LUAD) and (lung squamous cell carcinoma, LUSC) was analyzed using TCGA data (GAPIA, gene expression profiling interactive analysis; http://gepia.cancer-pku.cn/ and http://gepia.cancer-pku.cn/detail.php?gene=USP15).
Statistical analysis
All in vitro data are expressed as mean ± SEM of triplicate samples. Statistical significance was analyzed with ANOVA or Student’s t-test using GraphPad Prism 5.0 (GraphPad Software, San Diego, CA, USA).
Results
USP15 is significantly downregulated in patients with lung adenocarcinoma and negatively implicated in lung cancer invasion
To address the functional role of USP15 in human cancers, we first evaluated the expression of USP15 in human pan-cancer using the Cancer Genome Atlas (TCGA) data provided by GEPIA (Gene Expression Profiling Interactive analysis, http://gepia.cancer-pku.cn/detail.php?gene=USP15). Among 33 different human cancer types based on molecular similarity (Fig. 1A), five different cancer types, lung adenocarcinoma (LUAD), lung squamous cell carcinoma (LUSC), ovarian serous cystadenocarcinoma (OV), skin cutaneous melanoma (SKCM), and testicular germ cell tumors (TGCT), showed significantly downregulated expression of USP15 (Fig. 1B, tumor (T) vs. normal (N)), whereas no significant change in expression of USP15 was observed in 28 other cancer types (Fig. 1A, black letters). The expression of USP15 was markedly lower in those with lung cancer than in normal controls (Fig. 1C, 27.29 in lung cancer vs. 64.76 in normal controls). In addition, the survival rate from combined patients with LUAD and LUSC decreased in patients with low expression levels of USP15 (Supplementary Fig. S1, blue line with low USP15 vs. red line with high USP15), indicating that USP15 expression may have an essential role in lung cancer patient’s survival. To verify the TCGA cancer data in those with primary lung cancer, we assessed the expression of USP15 in 41 patients with primary non-small cell lung cancer (NSCLC) [18, 19], including 25 patients with lung adenocarcinoma (LUAD, blue bars in Fig. 1D), 8 patients with lung squamous cell carcinoma (LUSC, red bars in Fig. 1D), and 8 patients with other lung cancers (green bars in Fig. 1D). LUAD patients showed significant downregulation of USP15 (Fig. 1D, blue bars). These findings suggest that USP15 might be functionally associated with lung adenocarcinoma. Therefore, we explored whether the expression of USP15 was implicated in the progression of human lung adenocarcinoma. To explore the functional role of USP15 in human lung adenocarcinoma, we generated USP15-knockout (USP15KO) human lung adenocarcinoma A549 and USP15KO human non-small cell lung cancer H1299 cells using CRISPR/Cas9 gene-editing method (Fig. 2A, USP15KO A549; Fig. 2B, USP15KO H1299) as described in “Materials and methods”. With USP15KO A549 and USP15KO H1299 lung cancer cells, we preliminary assessed whether USP15 deficiency affected lung cancer invasion using a transwell invasion assay. The invasion of USP15KO A549 cells was significantly increased than that of control (Ctrl) A549 cells (Fig. 2C, D, USP15KO A549 vs. Ctrl A549 cells). Similarly, the invasion ability of USP15KO H1299 cells was also significantly higher than that of Ctrl H1299 cells (Fig. 2E, F, USP15KO H1299 vs. Ctrl H1299 cells), suggesting that USP15 might be functionally implicated in lung cancer invasion.
USP15KO A549 lung cancer cells exhibit increased lung cancer progression induced by TLR4 stimulation through enhanced autophagy
Previous reports have demonstrated that TLR3/4 activation can increase the production of IL-6, CCL2, MMP2, and CCL20 cytokines by promoting TRAF6 ubiquitination and autophagy induction, thereby facilitating the migration and invasion of lung cancer cells [7, 10, 11]. Having found that USP15KO A549 and USP15KO H1299 lung cancer cells exhibited increased cancer invasion, we further examined whether USP15 affected lung cancer migration and invasion through autophagy induction by TLR4 stimulation. The level of LC3-II specifically associated with autophagosomes and autolysosomes [28] was markedly enhanced in USP15KO A549 cells treated with LPS than in Ctrl A549 cells treated with LPS (Fig. 3A, USP15KO A549 with LPS vs. Ctrl A549 with LPS). In addition, the basal level of LC3-II/-I increased in USP15KO A549 cells without LPS (Supplementary Fig. S2, USP15KO A549 vs, Ctrl A549), indicating that the expression of USP15 affects the generation of LC3-II at the basal statue under the absence of LPS stimulation. As expected, autophagy inhibitors 3-MA (an inhibitor of phosphatidylinositol 3-kinases (PI3K) that can induce a decrease of LC3-II in cells) [29] and CQ (known to block the binding of autophagosomes to lysosomes and induce accumulation of LC3-II in cells) [30] induced a decrease and an increase of LC3-II in both cells treated with LPS, respectively (Fig. 3A, lane 2 vs. lane 3 or lane 4 in Ctrl A549 cells; lane 6 vs. lane 7 or lane 8 in USP15KO A549). Importantly, cancer invasion induced by TLR4 stimulation was markedly enhanced in USP15KO A549 cells treated with LPS, whereas it was inhibited by co-treatment of LPS with 3-MA or CQ (Fig. 3B, C, LPS vs. vehicle, or LPS + 3-MA and LPS + CQ vs. LPS in Ctrl A549 and USP15KO A549). Similar results were found for cancer migration (Fig. 3D, E, USP15KO A549 vs. Ctrl A549). Autophagy induced by TLR4 or TLR3 activation can enhance the production of cytokines such as IL-6, CCL2, MMP2, and CCL20 by promoting TRAF6 ubiquitination, thus facilitating the migration and invasion of lung cancer cells [7, 10, 11]. Therefore, we assessed whether USP15 was associated with the production of these cytokines induced by TLR4 stimulation. Interestingly, expression levels of MMP2, IL-6, CCL2, and CCL20 induced by TLR4 stimulation were significantly elevated in USP15KO A549 cells than in Ctrl A459 cells (Fig. 4A, MMP2; Fig. 4B, IL-6; Fig. 4C, CCL2; Fig. 4D, CCL20). These results suggest that USP15 is negatively implicated in the migration and invasion of lung cancer through the regulation of autophagy in response to TLR4 stimulation.
USP15KO H1299 lung cancer cells exhibit increases in cancer migration and invasion induced by TLR4 stimulation
Given the above results of USP15KO A549 cells, we verified the role of USP15 in USP15KO H1299 human non-small cell lung cancer cells. The migration of USP15KO H1299 cells was significantly enhanced in the presence or absence of LPS than that of Ctrl H1299 cells (Fig. 5A, B, USP15KO H1299 cells treated with vehicle or LPS vs. Ctrl H1299 cells treated with vehicle or LPS). Furthermore, cancer invasion was significantly higher in USP15KO H1299 cells treated with LPS than in Ctrl H1299 cells treated with LPS (Fig. 5C, D, USP15KO H1299 with LPS vs. Ctrl H1299 with LPS). Consistent with these results in USP15KO A549 cells (Fig. 3B–E), co-treatment of cells with LPS and 3-MA or CQ resulted in marked decreases of cancer migration and invasion induced by LPS (Fig. 5A, B, LPS vs. LPS + 3-MA or LPS + CQ; Fig. 5C, D, LPS vs. LPS + 3-MA or LPS + CQ). Moreover, expression levels of MMP2, CCL2, CCL20, and IL-6 induced by LPS stimulation were significantly elevated in USP15KO H1299 cells than in Ctrl H1299 cells (Fig. 5E, MMP2; Fig. 5F, CCL2; Fig. 5G, CCL20; Fig. 5H, IL-6 in USP15KO H1299 cells treated with LPS vs. Ctrl H1299 cells treated with LPS). These results strongly suggest that the deficiency of USP15 in lung cancer enhances cancer migration and invasion induced by TLR4 stimulation by increasing autophagy induction as depicted in Fig. 5I.
USP15 interacts with BECN1 and induces deubiquitination of BECN1
Having shown that USP15 negatively regulated lung cancer migration and invasion induced by TLR4 stimulation through inhibition of autophagy induction, we next explored the molecular mechanism in which USP15 was implicated in autophagy induction by TLR4 stimulation. Ubiquitination of BECN1 by TRAF6 plays a pivotal role in autophagy induction in response to TLR4 stimulation [7, 10, 11]. In addition, we have recently reported that USP15 can dampen NF-κB activation through deubiquitination of IκBα [16]. Therefore, the regulatory mechanism of USP15 in autophagy induction might be functionally associated with the ubiquitination of BECN1. Interestingly, USP15 interacted with BECN1 (Fig. 6A, lane 4), whereas no significant interaction was observed between USP15 and TRAF6 (Fig. 6B, lane 4). To elucidate the molecular association between USP15 and BECN1, BECN1 truncated mutants were generated (Fig. 6C), and immunoprecipitation (IP) assay was performed with USP15. FLAG-USP15 interacted with MYC-BECN1 wild-type (WT) and MYC-BECN1 1-269 mutant (Fig. 6D, lanes 6 and 7), whereas it showed a weak interaction with MYC-BECN1 1-127 (Fig. 6D, lane 8). To confirm the interaction between USP15 and BECN1 1-127 mutant, IP assay was further performed. As shown in Fig. 6E, FLAG-USP15 interacted with MYC-BECN1 WT and MYC-BECN1 1-127 mutant (Fig. 6E, lanes 4 and 5), indicating that USP15 could interact with the N-terminal domain of BECN1 (Fig. 6F).
To further verify the molecular interaction between USP15 and BECN1, we performed a semi-endogenous IP assay because the anti-USP15 antibody used in this study was not suitable for IP assay. FLAG-USP15 was transiently expressed in A549 and H1299 lung cancer cells, and then the interaction with endogenous BECN1 was evaluated (Fig. 7A, A549; Fig. 7B, H1299). FLAG-USP15 interacted with endogenous BECN1 in the absence of LPS stimulation, and the interaction was significantly enhanced in the presence of LPS stimulation (Fig. 7A, B, lane 4 vs. lane 3), indicating that USP15 interacts with endogenous BECN1. Based on the molecular association between USP15 and BECN1, we asked whether USP15 could induce deubiquitination of BECN1. FLAG-BECN1 and HA-Ub were transfected in the presence or absence of MYC-USP15. The ubiquitination of BECN1 appeared in the absence of MYC-USP15 (Fig. 7C, lane 2 vs. lane 1), whereas it was markedly attenuated in the presence of MYC-USP15 (Fig. 7C, lane 3 vs. lane 2). To verify whether the deubiquitination of BECN1 was dependent on the catalytic activity of USP15, we generated two catalytic mutants of USP15, USP15 C269A, and USP15 H862A [25]. A ubiquitination assay was then performed. Consistently, the wild-type USP15 induced deubiquitination of BECN1 (Fig. 7D, lane 3 vs. 2), whereas the two catalytic mutants of USP15 failed to induce deubiquitination of BECN1 (Fig. 7D, lanes 4 and 5 vs. 3), indicating that the deubiquitination of BECN1 was dependent on the catalytic activity of USP15. As depicted in Fig. 7E, these results suggest that the molecular association of TRAF6-BECN1 can lead to ubiquitination of BECN1 and induce autophagy induction, whereas the interaction of USP15 with BECN1 can induce the deubiquitination of BECN1 and inhibit autophagy induction.
Downregulation of USP15 is associated with genes related to lung cancer progression in patients with primary non-small cell lung cancer (NSCLC)
Given the results that USP15 negatively regulated lung cancer migration and invasion through inhibition of autophagy induction by deubiquitination of BECN1, we tried to find the clinical relevance of USP15 in the regulation of lung cancer progression. To do that, four patients with primary adenocarcinoma who showed significantly downregulated USP15 (< −1.5 values in the relative expression of USP15 in tumor and matched normal tissues) were selected (Fig. 8A, LTT26, LTT10, LTT12, and LTT35) among 41 patients with primary non-small cell lung cancer (NSCLC) (Fig. 1D). H&E staining presented significant pathological differences between matched normal and lung tumor tissues in terms of the structure of lung tissue and the number of cancer cells (Fig. 8B, normal vs. tumor). Given these results, microarray analysis was performed for matched normal and lung tumor tissues using Human HT-12 expression BeadChips. To select upregulated and/or downregulated genes in four LTTs, LTT26 tumor patient with the lowest expression of USP15 was used as a standard sample (Fig. 8C and Supplementary Table S1, upregulated genes; Fig. 8D and Supplementary Table S2, downregulated genes). Among 17 commonly upregulated genes in four LTT patients (Fig. 8E and Supplementary Table S3), 15 genes were functionally associated with lung cancer growth, proliferation, migration, invasion, or metastasis: 10 genes (CCNE1 [31], MMP9 [32], SFN [33], UBE2C [34], CCR2 [35], FAM83A [36], ETV4 [37], MYO7A [38], MMP11 [39], and GSDMB [40]) can promote lung cancer progression, two genes (DEPDC1B [41] and GALNT6 [42]) can promote lung cancer migration and invasion, and three genes (KIF18A [43], ADAM8 [44], and PHLDA2 [45]) can promote lung cancer proliferation, metastasis, or oncogenesis. Among 13 commonly downregulated genes in four patients (Fig. 8F and Supplementary Table S4), 10 genes (FMO2 [45], ZBTB16 [46], FCN3 [47], TCF21 [48], SFTPA1B [49], HPGD [50], SOSTDC1 [51], TMEM100 [52], GDF10 [53], and WIF1 [54]) have been reported as tumor suppressors in lung cancer. These results suggest that downregulation of USP15 in lung cancer might be associated with gene expression capable of affecting lung cancer progression and formation.
Discussion
This study demonstrates that USP15 is negatively implicated in lung cancer progression through the regulation of TRAF6-BECN1 signaling for autophagy induction for the first time. Importantly, TCGA data analysis revealed that USP15 was significantly downregulated in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Consistently, the expression of USP15 was significantly downregulated in patients with primary lung adenocarcinoma. Notably, the profound impact of USP15 expression was shown in USP15KO A549 and USP15KO H1299 lung cancer cells. USP15KO lung cancer cells exhibited increased cancer migration and invasion induced by TLR4 stimulation through the regulation of autophagy. Biochemical studies have revealed that USP15 can induce the deubiquitination of BECN1, a key regulator of the induction of autophagy [7, 10,11,12]. More importantly, microarray analysis in four patients with primary lung adenocarcinoma who had low expression of USP15, revealed that 10 different genes (CCNE1, MMP9, SFN, UBE2C, CCR2, FAM83A, ETV4, MYO7A, MMP11, and GSDMB) that are associated with promoting lung cancer progression were significantly upregulated, whereas 10 different genes (FMO2, ZBTB16, FCN3, TCF21, SFTPA1B, HPGD, SOSTDC1, TMEM100, GDF10, and WIF1) known to suppress lung cancer were markedly downregulated.
TRAF6 signals are known to play critical roles in differential biological contexts including innate and adaptive immunity and tumor development [8]. A distinct TRAF6-mediated signaling pathway has been revealed through its seemingly innumerable interaction partners and its E3 ubiquitin ligase activity [8]. Recent studies have shown that the TRAF6-BECN1 signaling axis plays a key role in the induction of autophagy, thereby promoting lung cancer migration and invasion in response to TLR4 stimulation [7, 10,11,12]. Importantly, autophagy functionally facilitates TLR4-induced lung cancer progression by enhancing TRAF6-mediated ubiquitination of BECN1 and the production of IL-6, CCL2/MCP-1, CCL20/MIP-3α, VEGFA, and MMP2 [7, 10,11,12], strongly suggesting a pivotal role of the TRAF6-BECN1 signaling axis in lung cancer progression. In the current work, we found that USP15 interacted with BECN1, but not with TRAF6. The interaction between USP15 and BECN1 led to the deubiquitination of BECN1, resulting in the attenuation of autophagy induction. Similarly, we have previously reported that USP15 can inhibit NF-κB activation induced by RANK signaling through deubiquitination of IκB-α [16], supporting that USP15 is a deubiquitinating enzyme that is functionally implicated in various cellular contexts.
Regarding the cellular function of USP15 in lung cancer progression, notably, USP15KO A549 and USP15KO H1299 lung cancer cells exhibited increases in cancer invasion that were markedly enhanced in response to TLR4 stimulation after treatment with LPS. In contrast, co-treatment of these cells with LPS and autophagy inhibitors such as 3-MA and CQ attenuated cancer migration and invasion induced by TLR4 stimulation. Based on the molecular mechanism by which USP15 is negatively implicated in the autophagy induction through deubiquitination of BECN1, our study suggests that the negative regulation of USP15 in lung cancer progression might be functionally implicated in the regulation of autophagy induction. In terms of clinical aspects, we found that the expression of USP15 was significantly downregulated in those with LUAD and LUSC. Consistently, USP15 was significantly downregulated in patients with primary lung adenocarcinoma, strongly suggesting that USP15 might be functionally associated with lung cancer. Importantly, patients with low expression levels of USP15 showed increased expression of 17 different genes related to cancer progression and formation and decreased expression of 13 different genes known to suppress tumors. Among these 17 different genes, 10 genes (CCNE1, MMP9, SFN, UBE2C, CCR2, FAM83A, ETV4, MYO7A, MMP11, and GSDMB) are known to promote lung cancer progression and formation. Among the 13 different genes known to suppress tumors, 10 genes (FMO2, ZBTB16, FCN3, TCF21, SFTPA1B, HPGD, SOSTDC1, TMEM100, GDF10, and WIF1) are known to suppress lung cancer. These results suggest that USP15 is negatively implicated in the progression and formation of lung cancer.
Autophagy plays a dual role in cancer development by suppressing the growth of tumors or cancer progression [55, 56]. The upregulation of autophagy leads to the resistance of cancer cells to various anticancer drugs [56, 57]. Upon cellular stimuli including TLRs, IL-17R, and TNFR, TRAF6 regulates tumor cell proliferation, survival, apoptosis, and invasion through different signaling pathways [8, 58]. As depicted in Fig. 9, TRAF6 has oncogenic characteristics involved in cancer progression. Importantly, recent studies have reported that autophagy facilitates TLR-induced migration and invasion of lung cancer cells through the TRAF6-induced ubiquitination of BECN1 [7, 10,11,12], suggesting that TLR-induced autophagy is functionally associated with lung cancer progression. On the other hand, accumulated evidence has proven that TLRs play a critical role in inducing anti-tumor effects by eliciting inflammatory cytokine expressions and innate immune response through the activation of NF-κB [59]. Although TLRs are essential for the immune response against tumor cells, recent studies have demonstrated that TLRs are expressed on tumor cells and crucially functioned in tumorigenesis and tumor progression through their downstream signaling pathways [7, 10,11,12, 59]. Indeed, TLRs act as critical sensors that regulate lung cancer progression including cell growth and invasion and migration and metastasis through the activation of NF-κB and autophagy [7, 10,11,12]. Although the precise roles and fundamental mechanisms by which TLR-induced autophagy is implicated in lung cancer progression need to be further elucidated, the pathway of TLR-induced autophagy can be potentially considered as a therapeutic target for alleviating lung cancer progression.
In summary, we proposed a possible mechanism by which USP15 regulates TRAF6-BECN1 signaling in autophagy induction and lung cancer progression. As depicted in Fig. 9, regarding the TRAF6-BECN1 signaling axis for cancer progression in response to TLR4 stimulation (depicted in the left), TRAF6 can interact with BECN1 and induce ubiquitination of BECN1, leading to the induction of autophagy (indicated as a black arrow). USP15 can interact with BECN1 and induce deubiquitination of BCEN1, thereby inhibiting the induction of autophagy (indicated as red dashed arrow). Eventually, USP15 can negatively regulate lung cancer progression by inhibiting the TRAF6-BECN1 signaling axis through autophagy induction. Although significant progress has been achieved in exploring the roles of USPs in cancers, very little is known about the molecular and cellular mechanism by which USPs are implicated in lung cancer progression and formation. Our current results provide insight into pathological lung cancer processes and the future development of therapeutic agents for treating lung cancer.
Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We would like to thank Hyehwa Forum members for their helpful discussion.
Funding
This work was supported by the National Research Foundation of Korea (NRF) Grants funded by the Korean Government (NRF-2021R1F1A1049324, NRF-2020R1A2C2007652, and NRF-2021R1A2C1094478), Korea Basic Science Institute (National Research Facilities and Equipment Center) grant funded by the Ministry of Education (2020R1A6C101A191), Ministry of Science ICT and Future Planning (MSIP) funded by the Korean Government (NRF-2016R1A5A2945889).
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M-JK, YM, S-KJ, JS, JYK, and JSL performed the experiments. M-JK and YM analyzed and interpreted the data. D-HK, JSL, EC, and K-YL analyzed TCGA and RNA-seq data. EC and K-YL designed the experiments and wrote the manuscript. EC and K-YL supervised the laboratory studies.
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Tumor and matched normal tissues from 41 patients with primary NSCLC were obtained in accordance with the ethical principles stated in the Declaration of Helsinki. This study was approved by the Institutional Review Board (IRB) of Samsung Medical Center (SMC) (IRB#: 2010-07-204). We obtained written informed consent from each patient before surgery for using their pathological specimens for research use.
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Kim, MJ., Min, Y., Jeong, SK. et al. USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction. Cell Death Dis 13, 348 (2022). https://doi.org/10.1038/s41419-022-04808-7
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DOI: https://doi.org/10.1038/s41419-022-04808-7
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