Abstract
The Na+-pumping NADH-ubiquinone oxidoreductase (Na+-NQR) couples electron transfer from NADH to ubiquinone with Na+-pumping, generating an electrochemical Na+ gradient that is essential for energy-consuming reactions in bacteria. Since Na+-NQR is exclusively found in prokaryotes, it is a promising target for highly selective antibiotics. However, the molecular mechanism of inhibition is not well-understood for lack of the atomic structural information about an inhibitor-bound state. Here we present cryo-electron microscopy structures of Na+-NQR from Vibrio cholerae with or without a bound inhibitor at 2.5- to 3.1-Å resolution. The structures reveal the arrangement of all six redox cofactors including a herein identified 2Fe-2S cluster located between the NqrD and NqrE subunits. A large part of the hydrophilic NqrF is barely visible in the density map, suggesting a high degree of flexibility. This flexibility may be responsible to reducing the long distance between the 2Fe-2S centers in NqrF and NqrD/E. Two different types of specific inhibitors bind to the N-terminal region of NqrB, which is disordered in the absence of inhibitors. The present study provides a foundation for understanding the function of Na+-NQR and the binding manner of specific inhibitors.
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Introduction
The Na+-pumping NADH-ubiquinone (UQ) oxidoreductase (Na+-NQR) couples electron transfer from NADH to UQ with Na+-pumping, generating an electrochemical Na+ gradient across the inner bacterial membrane, which is critical for energy-consuming reactions such as rotation of the flagellar motor, ion homeostasis, and uptake of nutrients1,2,3. Na+-NQR is the first enzyme in the respiratory chain of many pathogenic bacteria such as Vibrio cholerae, Vibrio alginolyticus, and Haemophilus influenzae. This enzyme is an intrinsic membrane protein complex composed of six subunits (NqrA–F), encoded by the nqr operon, with a total molecular mass about 200 kDa, and contains at least five redox cofactors (FAD, a 2Fe-2S cluster, two covalently bound FMNs, and riboflavin). There is a consensus that the electron transfer takes place through the enzyme following the pathway: NADH → FADNqrF → 2Fe-2SNqrF → FMNNqrC → FMNNqrB → riboflavinNqrB → UQ4,5,6,7,8,9,10,11,12,13. Since Na+-NQR is exclusively found in prokaryotes and is structurally unrelated to mitochondrial H+-pumping NADH-UQ oxidoreductase (respiratory complex I), it is a promising target for highly selective antibiotics3,14,15.
Steuber et al.16 reported an X-ray crystallographic structure of selenomethionine-labeled V. cholerae Na+-NQR at 3.5-Å resolution, which was built by placing the crystallographic structures of individual soluble domains of NqrA, NqrC, and NqrF into the low-resolution experimental electron density map. This structure provided valuable information about the overall architecture of the enzyme, though it included no bound UQ or inhibitor. The distances between several pairs of redox cofactors in the crystallographic structure (e.g. between FMN and riboflavin of NqrB) are too long (29–32 Å (edge-to-edge)) to support physiologically relevant electron transfer16,17. Therefore, it has been suggested that the subunits harboring the cofactors undergo large conformational changes to decrease these spatial gaps during catalytic turnover16. In fact, the linker regions between the single transmembrane helix, the N-terminal ferredoxin-like domain, and the FAD-binding domain in NqrF were barely visible in the electron density map, suggesting a high degree of flexibility. In addition, the crystallographic study modeled an extra cofactor, a single iron coordinated by four cysteine residues, two in NqrD (Cys29 and Cys112) and two in NqrE (Cys26 and Cys120), that was proposed to be an extra redox carrier, facilitating electron transfer between 2Fe-2SNqrF to FMNNqrC. However, the presence of this iron center and its redox function remain to be verified by further experiments.
Based on photoaffinity labeling experiments18,19, we previously showed that korormicin A (a highly specific inhibitor of Na+-NQR18,20,) and aurachin D-type inhibitors (naphthoquinone-like compounds) bind to a part of the protruding N-terminal stretch starting with transmembrane helix (TMH) 1 of NqrB (Trp23 − Lys54, Supplementary Fig. 1) and that the UQ head-ring binds to the cytoplasmic region of NqrA close to the N-terminal stretch of NqrB (Leu32 − Met39 and Phe131 − Lys138, Supplementary Fig. 1). Thus, the N-terminal stretch of NqrB (Met1 − Lys54) may be critical for regulating the UQ reaction at the adjacent NqrA. However, since approximately three-quarters (Met1 − Pro37) of the protruding N-terminal stretch of NqrB was not modeled in the crystallographic structure16, the structural and functional roles of this key segment remain unclear. Therefore, high-resolution structures of Na+-NQR are essential for understanding how all the cofactors are arranged in six subunits, how they participate in redox-coupled Na+ translocation, and how inhibitor molecules bind to the N-terminal region of NqrB.
Here, we present high-resolution (2.5–3.1 Å) structures of the oxidized form of V. cholerae Na+-NQR in three different conditions determined by single-particle cryo-electron microscopy (EM): the native enzyme, without bound UQ or inhibitor, and enzymes with korormicin A or aurachin D-42 bound to NqrB. Although the structure of the N-terminal region of NqrB is disordered in the absence of inhibitor, its structure with bound korormicin A or aurachin D-42 could be fully assigned, including the missing N-terminal stretch of NqrB, because the binding of the inhibitors made the region rigid. As we previously predicted, based on chemistry-based studies21, the N-terminal region orients itself toward the membrane phase by folding up at the cytoplasmic membrane surface rather than protruding into cytoplasmic medium along the surface of NqrA. Furthermore, our structures reveal that FMNNqrC, FMNNqrB, and riboflavinNqrB embedded in the membrane phase, are closer to one another than in the X-ray crystallographic structure16. The present study provides insights not only into the overall structural rearrangements of Na+-NQR but also into the molecular details of binding of specific inhibitors.
Results
Overall cryo-EM structure of Na+-NQR without bound inhibitor
The recombinant Na+-NQR from V. cholerae was expressed in a V. cholerae strain lacking the genomic nqr operon and purified by affinity chromatography in the presence of n-dodecyl-β-D-maltoside (DDM). Using the purified Na+-NQR, we determined its cryo-EM density map at 2.7-Å overall resolution (Supplementary Figs. 2 and 3). The initial map was of sufficient quality to build an atomic model of most part of the enzyme. However, the density map of the region in the cytoplasmic NqrF subunit, facing the membrane-embedded subunits (NqrE and NqrD), was relatively weak and blurry compared to other parts of the enzyme. This was also the case for the X-ray crystallographic structure16. We can confidently attribute this weak density to structural flexibility of this region of NqrF, rather than loss of the subunit from the complex, because we have confirmed that our preparation of Na+-NQR is intact and active, as judged by NADH-UQ1 oxidoreductase activity that is fully sensitive to inhibitor, and the presence of all six subunits (NqrA to NqrF) on SDS-PAGE (Supplementary Fig. 4).
To obtain the structure of the cytoplasmic domain of NqrF, focused 3D classification without angular alignment, following local refinement on the NqrA and NqrB subunits was carried out. As expected, the cytoplasmic domain of NqrF showed high flexibility compared to other subunits (Supplementary Movie 1). The focus 3D classification resulted in 10 classes; we selected the three, in which NqrF was clearly observed (Supplementary Fig. 2). The particles belonging to the three classes were applied further Non-uniform refinement. As a result, we obtained high-resolution structures of states 1, 2, and 3 from 82,790, 80,882, and 72,234 particles, respectively, at 3.1-Å resolution (Supplementary Fig. 2). Atomic models of the cytoplasmic domain of NqrF of the three states were built from the resulting density maps. Figure 1A and B show the density map and the atomic model of Na+-NQR, respectively, taking state 1 as a representative. The atomic models for three states are almost identical except for the flexible cytoplasmic domain of NqrF. Details of data processing and statistics for the maps and models are summarized in Supplementary Fig. 2 and Supplementary Tables 1 and 2.
The final high-quality density map allowed us to build an essentially complete model of all six subunits containing 1894 residues (98% of the total), except for the disordered N-terminal region (Gly2 − Leu26) of NqrB. However, this region was well modeled in the inhibitor-bound enzyme, as described later. We note that the N-terminal amino acid of mature NqrB was determined to be glycine, not methionine, by direct Edman degradation21. The NqrB, NqrD and NqrE subunits are integral membrane proteins that have 10, 6, and 6 TMHs, respectively. NqrC and NqrF are hydrophilic proteins each anchored by single TMH. A hydrophilic NqrA contains no TMH. The present study clearly defined all 24 TMHs (Fig. 1C). The structures of the membrane domain in our model are almost identical to those in the crystallographic structure16. Nevertheless, there are some differences in the local structures of TMHs between the two models (Fig. 1C). TMH 2NqrD, TMH 3NqrD, TMH 1NqrC, and TMH 1NqrF, which form a helix bundle, are slightly shifted together toward the outside of the enzyme compared to the crystallographic structure. TMH 3NqrD and TMH 4NqrD slightly bent toward the center of the enzyme. The cytosolic region of TMH 3NqrE and TMH 4NqrE is shifted about 10 Å toward the outside of the enzyme. Furthermore, a membrane interior loop (Gly266–Ser276) lies in a narrow central cavity of NqrB (red in the leftmost inset); although this region was modeled as a part of TMH 6 and its periplasmic loop in the crystallographic structure.
The crystallographic study could not resolve some parts of the loops connecting TMHs 1–2, 5–6, 7–8 of NqrB, TMHs 2–3, 3–4, 4–5, 5–6 of NqrD, and TMHs 2–3 of NqrE. The C-terminal loop region of NqrA (Arg330–Val375), which is critical to attach this subunit to the membrane by anchoring to the cytoplasmic surface of NqrB, was also not modeled. The present study successfully resolves all of the loops. On the whole, the overall cryo-EM structure of Na+-NQR closely resembles the crystallographic structure16. In fact, the r.m.s.d. values for each subunit between the two structures are less than 2.5 Å (Supplementary Table S3). Nevertheless, the most notable difference is that in our models, the periplasmic hydrophilic domain of NqrC, which accommodates FMNNqrC, significantly shifts towards NqrB compared to that in the crystallographic structure, as discussed later. Altogether, the present high-resolution cryo-EM structures resolves some critical unsettled questions in the crystallographic structure.
Structural basis of anchoring the cytoplasmic hydrophilic subunits NqrA and NqrF to the membrane
A large region of the hydrophilic NqrF subunit facing the membrane-embedded subunits is barely visible in the density map, suggesting a high degree of flexibility. As described in the above section, focused 3D classification visualized the cytoplasmic domain of NqrF in three different classes. NqrF binds tightly to the neighboring hydrophilic NqrA via electrostatic interactions at the interface of these subunits, where it is complementarily charged, as shown in Fig. 2 using the state 1 model. The positive patch in NqrA, formed by Arg40, Lys61, Lys62, Lys84, and Arg85, interacts with the negative patch in NqrF, formed by Glu368, Glu371, Asp372, Glu397, and Glu399. In addition, negative tips of NqrA (Glu443 and Glu445) are in close proximity to positive tips of NqrF (Lys100 and Arg104). These electrostatic interactions in the contact area are consistent across the three different classes of NqrF (Supplementary Fig. 5), suggesting that this contact area serves as the anchor that allows flexible structural changes of the rest of the hydrophilic parts of the subunit. In the structure of NqrF we can identify the NADH binding site, FAD and the 2Fe-2S center. The edge-to-edge distance between FADNqrF and 2Fe-2SNqrF is 10.8 Å, which is almost identical to that determined by the crystallographic structure (9.8 Å,16).
The hydrophilic NqrA subunit is attached to the cytoplasmic surface of the Na+-NQR complex through NqrB, but the details of this interaction have remained unclear because some parts of the interface between NqrA and NqrB were not resolved in the crystallographic structure16. Elucidation of this issue is critical to understand the mechanism by which UQ accepts electrons from the enzyme, since the binding site of the UQ head-ring lies in NqrA8,18. Our cryo-EM structure reveals that the C-terminal loop region of NqrA (Arg330 − Asn379) is intertwined with the almost all of the protruding part of the N-terminus of NqrB (Phe34 − Leu54) at the cytoplasmic membrane surface (Supplementary Fig. 6). In addition, a hydrophobic segment of the C-terminal loop of NqrA, that contains Leu334, Phe335, Trp337, Ala338, Pro340, Leu352, and Leu355, would be inserted into the membrane phase in vivo. Together, these interactions anchor NqrA to the membrane surface.
Revisiting the assignment of the redox cofactors
The intrinsic membrane subunits NqrD and NqrE are homologous proteins with a sequence identity of 37%. The X-ray crystallographic study reported that these two subunits form a two-fold pseudo-symmetrical dimer, resulting in the coordination of four highly conserved cysteine residues (NqrD-Cys29/NqrE-Cys120 and NqrE-Cys26/NqrD-Cys112) to a single iron atom at the center of the dimer surface. Although this iron atom appears to be critical for the electron transfer mediating between 2Fe-2SNqrF and FMNNqrC, the presence of this putative iron center has yet to be definitely verified by biochemical and biophysical characterizations13. However, in our cryo-EM structure, the density map at the interface between NqrD and NqrE shows a rhombus-shape resembling that of a 2Fe-2S cluster (Fig. 3), as observed in structures of other redox enzymes22,23. The density is coordinated by the two pairs of conserved cysteine residues described above, which are known as the typical ligands for a 2Fe-2S cluster22,23. These features strongly suggest that the density belongs to a 2Fe-2S cluster rather than a single iron atom; therefore, we have assigned it as 2Fe-2SNqrD/E. Based on site-directed mutagenesis, Fadeeva et al.24 reported that these conserved cysteine residues are necessary for the correct folding and/or the stability of Na+-NQR from Vibrio harvevi. The edge-to-edge distances between 2Fe-2SNqrD/E and FMNNqrC in our model and between FeNqrD/E and FMNNqrC in the crystallographic structure16 are 29.1 and 7.9 Å, respectively. Since the positions of 2Fe-2SNqrD/E and FeNqrD/E in the membrane phase are almost identical between the two structural models, this remarkable difference in the distance is due to shift of the hydrophilic domain of NqrC, in which FMNNqrC is bound, as described later. As the distances between 2Fe-2SNqrD/E and FMNNqrC is too long to support physiologically relevant electron transfer, the subunits harboring the two cofactors would undergo a large conformational changes to reduce the spatial gap during the catalytic turnover.
Riboflavin, a unique cofactor of Na+-NQR that forms a stable neutral semiquinone radical in the as-isolated state of the enzyme6,25, mediates the final electron transfer from FMNNqrB to UQ. Based on a biochemical study using the Na+-NQRs that lack individual subunits, Casutt et al.26 reported that riboflavin is localized to subunit NqrB. On the other hand, the crystallographic study assigned the riboflavin to a large patch of Fo–Fc density between NqrB and NqrE because no additional density was observed inside NqrB16. Thus, the position of riboflavin is still under debate. We can now definitely assign riboflavin to the density map inside NqrB, which is surrounded by central TMHs 1, 3, 5, and 8 (Fig. 4A and B). The riboflavin is accommodated in NqrB with the isoalloxazine ring oriented towards the inside of the protein via multiple hydrogen bonds with conserved polar amino acid residues. Thr162TMH3 and Asn203TMH5 form hydrogen bonds with the O2 and O4/N3 atoms of the isoalloxazine ring, respectively (Fig. 4C). The 3’-hydroxy group of the ribityl side chain is in the vicinity of the O2 and N1 atoms, suggesting this hydroxy group works as an intramolecular hydrogen bond donor to these atoms. The rest of the hydroxy groups in the ribityl chain (at the 2’-, 4’-, and 5’-positions) are close enough to Asn200TMH5 and Asp346TMH8 to form hydrogen bonds (Fig. 4C).
Comparison of the positions of all cofactors between the cryo-EM and crystallographic structures
The NqrC subunit is a periplasmic protein anchored by a single TMH that is in close contact with the TMH 3 of NqrD and TMH 1 of NqrF (Fig. 1C). The FMNNqrC that accepts electrons from the 2Fe-2SNqrD/E is covalently bound via a phosphoester bond to NqrC-Thr225 located in a helix of the C-terminus (Fig. 5A). The body of FMN protrudes out from the NqrC protein matrix and lies in a cavity formed by NqrB, NqrD, and NqrE on the periplasmic side of the membrane. The isoalloxazine ring of the FMN is sandwiched between NqrC-Leu145 and -Leu176, and its N5 atom is likely to be stabilized by NqrC-Trp146 and -Thr173. Compared with the crystallographic structure16, FMNNqrC and these residues are substantially shifted toward FMNNqrB and form a closed pocket at the interface between NqrB and NqrC, in which FMNNqrC and FMNNqrB face each other (Fig. 5A). This arrangement reduces the edge-to-edge distance between the two cofactors to 7.8 Å (21.4 Å in the crystallographic structure), which is short enough to achieve efficient electron transfer17. However, this same structural shift is also responsible for the long-distance (29.1 Å) between 2Fe-2SNqrD/E and FMNNqrC in our model (the distance between FeNqrD/E and FMNNqrC is 7.9 Å in the crystallographic structure).
The positions of all redox cofactors are compared between the present cryo-EM and X-ray crystallographic16 structures in Fig. 5B and C, although we have assigned the density between NqrD and NqrE as an 2Fe-2S cluster in place of a single iron. Even if we disregard the positions of the two cofactors (FAD and 2Fe-2S cluster) located in the very flexible NqrF for the time being, there is a pair of redox cofactors in each structure whose spatial distance is too long for efficient electron transfer, namely, 2Fe-2SNqrD/E and FMNNqrC and FMNNqrB and riboflavinNqrB in the cryo-EM and crystallographic structures, respectively. Nevertheless, as the position of riboflavinNqrB was corrected in this study, the spatial distances between the three flavin cofactors (FMNNqrC, FMNNqrB, and riboflavinNqrB) are significantly shortened, consistent with efficient electron transfer between them. Besides the flavin cofactors, the spatial distances between 2Fe-2SNqrF and 2Fe-2SNqrD/E and between 2Fe-2SNqrF and FeNqrD/E in the cryo-EM and crystallographic structures, respectively, are also too long. Clearly, the catalytic cycle must involve significant structural changes that modulate the distances between the cofactors and are likely to be important for the coupling between redox and Na+ translocating reactions. However, the single structural snapshot of an oxidized form of the enzyme provides only limited information about the dynamic structural changes. Having structures of the fully-reduced and various partially-reduced states of Na+-NQR would be expected to be of great help in elucidating these important questions.
Structures of Na+-NQR with bound inhibitor
To characterize the binding sites of inhibitors in V. cholerae Na+-NQR, we previously carried out photoaffinity labeling studies using korormicin and aurachin D-type derivatives (Supplementary Fig. 1B)18,19. The studies demonstrated that these inhibitors bind to the region Trp23 − Lys54 in the N-terminus starting with TMH 1 of NqrB and/or a part of the cytoplasmic loop (NqrB-His153 − Gly156) connecting TMHs 2–3 of NqrB. However, since most of the N-terminus of NqrB (Gly2 − Pro37) could not be resolved in the crystallographic structure16, leaving the molecular mechanism of the inhibitors unclear.
Therefore, we prepared cryo-EM grids of Na+-NQR in the presence of aurachin D-42 or korormicin A. Image processing provided the consensus density maps at 2.5- and 2.6-Å resolution for the aurachin D-42- and korormicin A-bound enzymes, respectively (Supplementary Fig. 2B and C). However, the density corresponding to the hydrophilic domain of NqrF in these maps was faint, as was in the map for the enzyme without inhibitor (Supplementary Fig. 2A and 3). Further 3D classification allowed us to obtain the final model at 3.0- and 2.9-Å resolution for the aurachin D-42- and korormicin A-bound enzymes, respectively (Supplementary Table 2). In both cases, we successfully modeled the entire N-terminus of NqrB, in which the inhibitors are accommodated (Fig. 6A and B). The loop connecting TMHs 2–3 of NqrB forms a part of the rear wall of the binding cavity. These results are consistent with the results of our photoaffinity labeling studies18,19. The N-terminal region (Gly2 − Leu26) of NqrB is flexible and disordered in the absence of an inhibitor, as mentioned above, whereas the binding of inhibitor gives rise to the distinct conformation that we could identify by cryo-EM (Fig. 6A and B). It is noteworthy that the N-terminal region starting with TMH 1 first protrudes from the membrane phase and then turns back toward the membrane (bending at Arg43) and forms the binding cavity for inhibitors by folding up at the cytoplasmic membrane surface (Supplementary Fig. 7A and B). Based on a pinpoint chemical modification study of Na+-NQR, we previously proposed that the N-terminal region of NqrB is oriented toward the membrane phase rather than protruding into the cytoplasmic medium along the surface of NqrA21, as implied by the crystallographic structure16. The present cryo-EM structure unambiguously corroborates this view. We note that the three cryo-EM structures of Na+-NQR (i.e. the enzyme without bound inhibitor and the enzymes with bound korormicin A or aurachin D-42) are almost identical except for the N-terminal region of NqrB, which is disordered in the absence of inhibitor (Supplementary Fig. 7A and C).
The binding model of aurachin D-42 (a naphthoquinone-like inhibitor) is shown in Fig. 6A and Supplementary Fig. 8A. The NH group on the quinolone ring forms a hydrogen bond with NqrB-Glu157 via an intervening water molecule. The configuration of NqrB-Glu157 may be fixed by a hydrogen bond with the adjacent NqrB-Lys54 via a water molecule. NqrB-Phe160 is involved in a π-stacking interaction with the quinolone ring, which may lock aurachin D-42 inside the binding cavity (Supplementary Fig. 8A). The farnesyl chain bends at the second isoprene unit and turns toward the membrane phase to avoid steric obstruction arising from the cavity wall formed by TMH 2.
The binding model of korormicin A is presented in Fig. 6B and Supplementary Fig. 8B. The carbonyl group on the lactone ring and the amide NH form hydrogen bonds with NqrB-Glu157 via an intervening water molecule. The configuration of the lactone ring, which has alkyl branches (CH3/C2H5) at the 5-position, relative to the adjacent amide plane appears to be restricted by steric obstruction arising from Met57 and Ile58 on TMH 1 and Phe160 on TMH 3 of NqrB (Supplementary Fig. 8B). The 3’-OH group forms a hydrogen bond with NqrB-Glu144, which is located in the rear wall of the binding cavity, via an intervening water molecule. The long alkyl side chain extends toward a hydrophobic cleft composed of Trp23, Leu26, Phe142, and Val145 of NqrB and is buried in it. No hydrogen-bond donating residue was identified near the oxygen atom of the epoxy group at the 9’/10’ positions, which orients toward the outside of the cavity. In contrast to the aurachin D-42-bound structure, NqrB-Phe160 does not face toward korormicin A.
The above results strongly suggest that NqrB-Glu157 plays a key role in the binding of the two inhibitors by making a hydrogen bond to their toxophoric moieties. To assess the significant contribution of NqrB-Glu157, we prepared a NqrB-Glu157Ala mutant and compared the inhibitory activities of korormicin A and aurachin D-42 to those measured with the wild-type enzyme. The NADH-UQ1 oxidoreductase activity of the isolated NqrB-Glu157Ala enzyme without inhibitor is 50–60% lower than that of the wild-type enzyme. This is probably because the binding site of UQ in NqrA, which may be located near the inhibitor binding site18, is influenced by the mutation. While IC50 values of korormicin A and aurachin D-42 determined in the NADH-UQ1 oxidoreductase assay with the wild-type enzyme were 5.0 and 2.0 nM, respectively18,19, 50% inhibition was not observed for either inhibitor with the mutant, even at 2.0 µM. These results indicate the critical contribution of NqrB-Glu157 to the inhibitor binding.
We previously sought a method to enable pinpoint chemical modification of amino acid residue located in the N-terminal region of NqrB. We found that N-acyl-N-alkyl sulfonamide chemistry (Supplementary Fig. 9A), one of the protein-ligand affinity-driven substitution techniques27, to be suitable for this purpose. Using this method, an electrophilic N-acyl-N-alkyl sulfonamide group attached to the end of the side chain of korormicin derivatives (NAS-K1 and NAS-K2, Supplementary Fig. 9B) specifically reacts with the nucleophilic NqrB-Lys2221. This result strongly suggests that NqrB-Lys22 is located near the side chain of the korormicin derivatives. In corroboration of this, in the cryo-EM structure, NqrB-Lys22 is located in the vicinity of the hydrophobic cleft that accommodates the side chain of korormicin (Supplementary Fig. 8B), although the position of the side chains of NAS-K1 and -K2 would not necessarily be identical to that of natural korormicin A because of their structural differences.
Discussion
A complete picture of the electron transfer reactions and the mechanism of Na+ translocation coupled to electron transfer in Na+-NQR remains elusive. High-resolution structures of Na+-NQR are essential for understanding these central questions. Here, we describe the cryo-EM structures of V. cholerae Na+-NQR with and without a bound inhibitor at 2.5- to 3.1-Å resolution. The present study provides information about the enzyme structure, which sheds light on unsettled issues in the earlier X-ray crystallographic study16. First, we revealed how the cytoplasmic water-soluble domains of the NqrA and NqrF subunits attach to the membrane-embedded subunits. The C-terminal region of NqrA (Arg330 − Asn379) is intertwined with the protruding part of the N-terminus of NqrB (Phe34 − Leu53) (Supplementary Fig. 6). The soluble domain of NqrF is anchored to the soluble domain of NqrA by tight electrostatic interactions (Fig. 2), which may allow the rest of NqrF to flexibly move. Second, riboflavin is located inside NqrB at the cytoplasmic side, where it is surrounded by central TMHs 1, 3, 5, and 8 (Fig. 4). Therefore, it is not conceivable that the Na+ channel traverses the center of NqrB16. Third, the unique density between NqrD and NqrE can be assigned to a 2Fe-2S cofactor (not a single iron atom), which is coordinated by the two pairs of the conserved cysteine residues (NqrD-Cys29/NqrE-Cys120 and NqrE-Cys26/NqrD-Cys112) (Fig. 3). Finally, we found that both korormicin A and aurachin D-42 bind to the N-terminal region of NqrB (Fig. 6), corroborating the results of our previous photoaffinity labeling experiments18,19. It is noteworthy that while the N-terminal region of NqrB (Gly2 − Leu26) is disordered in the absence of inhibitors, the binding of inhibitors gives rise to a distinct conformation that we could identify by cryo-EM. Altogether, the present cryo-EM study provides a definite foundation for understanding the function of Na+-NQR and the molecular mechanism of its specific inhibitors. To elucidate the predicted large structural changes during the catalytic turnover, individual cryo-EM structures of different partially-reduced states of the enzyme are necessary.
The UQ reduction is the final step in the overall electron transfer pathway in Na+-NQR. We recently found that the role of UQ is not simply to accept electrons from the reduced riboflavinNqrB to reset the catalytic cycle but critically regulates Na+-translocation via its side chain moiety28. Therefore, identification of the UQ reaction site is essential for fully understanding the overall electron transfer and the mechanism responsible for Na+-pumping driven by the electron transfer. It has been suggested, in some literature10,26,29, that the isolated Na+-NQR contains sub-stoichiometric amounts of a high affinity bound UQ8; however, the present cryo-EM structure presents no evidence for the existence of the bound UQ8. Photoaffinity labeling experiments using a photoreactive UQ showed that the UQ head-ring binds to the contact area of NqrA-Leu32 − Met39 and Phe131 − Lys13818. In the current cryo-EM structure of an oxidized form of Na+-NQR, this area forms a part of a cavity that is large enough to accommodate the UQ head-ring (Supplementary Fig. 10), but the cavity is ~20 Å above the predicted membrane surface. Therefore, the distance between the cavity and riboflavin located in NqrB is too long (~30 Å) to allow efficient electron transfer. Unfortunately, we have yet to obtain the cryo-EM structure of Na+-NQR with bound UQ8 and/or short-chain UQs such as UQ2. This is presumably because the binding affinities of substrate UQs to the enzyme are substantially lower compared to the potent inhibitors used in the present study. This challenging task is underway in our laboratory.
Steuber et al16. proposed that a membrane-spanning narrow cavity formed by the central TMHs 1, 3, 6, and 8 in NqrB is the Na+-channel, in which Na+ travels from the cytoplasmic to periplasmic sides. Based on the current cryo-EM structure, this possibility may be low because riboflavin lies at the center of NqrB on the cytoplasmic side, where it is surrounded by TMHs 1, 3, 5, and 8 (Fig. 4) and because the membrane interior loop (Gly266–Ser276), whose position is substantially different from that in the crystallographic structure16, covers the cavity interior facing the periplasmic side (Fig. 1C). Thus, there is no membrane-spanning cavity in the center of NqrB. Considering that Na+-NQR transports Na+ against the electrochemical gradient, it is not conceivable that a channel that completely traverses the membrane could exist in any active conformation of the enzyme. On the other hand, through biochemical characterization of the mutants (to Ala in most cases) of 17 conserved carboxylic acid residues located in the TMHs of Na+-NQR, Juárez et al.30 identified three acidic residues (NqrB-Asp397, NqrD-Asp133, and NqrE-Glu95) that are important for Na+ uptake. Electrochemically induced FTIR difference spectroscopic study of the wild-type and NqrB-Asp397Glu enzymes suggested that NqrB-Asp397 may be a ligand for Na+ binding31. The positions of the three residues are shown in the current cryo-EM structure (Supplementary Fig. 11). Although these residues are located on the cytoplasmic membrane surface, it is currently difficult to comprehensively elucidate their functions because they are far apart from each other.
Korormicin A is a highly specific and potent inhibitor of Na+-NQR, which exhibits no inhibition against the respiratory enzymes in mammalian mitochondria3,20. To identify important structural factors of korormicin A required for exhibiting this inhibition, we previously investigated the relationship between structure and inhibitory potency using a series of synthetic korormicin A analogs19, as summarized in Supplementary Fig. 12. The cryo-EM structure of the korormicin A-bound Na+-NQR allows us to understand how such structural factors are involved in the interaction with the enzyme. Here, we focus on the following three important structural factors: i) the alkyl branches (CH3/C2H5) at the 5-position, ii) R-configuration of the 3’-OH group, and iii) the epoxy group at the 9’/10’ positions.
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i.
The presence of the alkyl branches at the 5-position of the lactone ring is essential for the potent inhibition, as seen from the drastic decrease in the inhibitory potency of compound D3 (Supplementary Fig. 12). In unbound korormicin A, the lactone ring can freely rotate around the bond connecting it to the amide (-NH-CO-) moiety and, hence, does not take a fixed conformation relative to the amide plane. However, in the bound state, steric obstruction between the alkyl branches and the cavity wall formed by NqrB-Met57 and -Ile58 on TMH 1, NqrB-Phe160 on the TMH 3, and NqrA-Trp337 (Supplementary Fig. 8B) may fix the conformation of the lactone ring and make its carbonyl group oriented toward the inside of the binding cavity, enabling a hydrogen bond to NqrB-Glu157 via intervening water molecules (Fig. 6B).
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ii.
The inhibitory potency of the 3’-epimer of korormicin A markedly decreased (korormicin A vs. compound D4), indicating the importance of the R-configuration of the OH group at the 3’-position. This functional group may form a hydrogen bond with NqrB-Glu144 located in the rear wall of the binding cavity. (Fig. 6B and Supplementary Fig. 8B). In contrast, the OH group in S-configuration of compound D4 may be difficult to form such a hydrogen bond because this functional group does not orient toward the inside of the cavity.
-
iii.
The epoxy group at the 9’/10’ positions is favorable but not essential for inhibition (korormicin A vs. compound D5). Since no hydrogen-bond-donating residue is located near the epoxy oxygen atom, which orients toward the outside of the binding cavity (Fig. 6B), this favorable effect may not be due to a hydrogen bond. It is rather likely that the presence of the epoxy group adjacent to the 4’,6’-diene plane bends the alkyl side chain moiety, which enables the side chain to extend toward the hydrophobic cleft composed of NqrB-Trp23, -Leu26, -Phe142, and -Val145 by making a detour around the cavity wall formed by TMH 2 (Fig. 6B and Supplementary Fig. 8B).
The molecular interactions between aurachin D-42 and the binding cavity are slightly different from those for korormicin A described here (Fig. 6A vs B), suggesting that the N-terminal region of NqrB flexibly enfolds the inhibitors according to their unique chemical frameworks. Our cryo-EM structures of the inhibitor-bound Na+-NQR can help molecular design strategies for new inhibitors targeting the enzyme.
Finally, we discuss a unique phenomenon observed in the previous photoaffinity labeling study using photoreactive aurachin D-type inhibitors ([125I]PAD-1 and [125I]PAD-2, Supplementary Fig. 1B) with the isolated V. cholerae Na+-NQR18. The labeling by these inhibitors rather than being competitively suppressed in the presence of excess other inhibitors, was enhanced when a molar ratio of 125I-incorporated inhibitor to the enzyme is relatively low (<10). To explain this unusual competitive behavior, we proposed an equilibrium model for the binding of 125I-incorporated inhibitor and competitor based on the assumption that there are two distinct inhibitor-bound states; one-inhibitor- and two-inhibitor-bound states (ref. 18 for details). However, the current cryo-EM structures provided no evidence for the existence of two distinct binding sites for inhibitor; therefore, the equilibrium model must be corrected. The present study revealed that, while the N-terminal region (Gly2−Leu26) of NqrB is disordered in the absence of inhibitor, the binding of inhibitor gives rise to a distinct conformation of this region. It is, therefore, likely that the bound inhibitor shapes the region into its own binding cavity reflecting its chemical framework. This mode of binding may be thought of as the so-called “induced-fit” binding. Given that the inhibitor binding is a reversible equilibrium process, the binding cavity of the inhibitor may exist in, at least, two different conformations in equilibrium: one, disordered conformation with a low affinity to inhibitor and the other, distinct fixed conformation with a high affinity to inhibitor. The latter would correspond to the binding cavity identified by cryo-EM (Fig. 6A and B). Based on the idea of two different conformations of a single binding cavity, we produced an equilibrium binding model to explain the unusual competitive behavior. The details of this analysis are described in Supplementary Discussion. In conclusion, the concentrations of the enzyme-125I-incorporated inhibitor complex (“F-[125I]I” complex in Supplementary Fig. 13) change, from enhancement to suppression, as the concentration of competitor increases. This is because the added competitor, in a certain concentration range, increases the proportion of the binding cavity in the fixed conformation by reversibly binding to the enzyme. Thus, the unusual competitive behavior observed in the previous studies18 can be accounted for by an equilibrium model based on the idea of two different conformations of a single binding cavity.
Methods
Expression of wild-type and mutant Na+-NQRs
The recombinant Na+-NQR of V. cholerae, which contains a six-histidine affinity tag at the C-terminus of NqrF subunit, was produced in a V. cholerae strain lacking the genomic nqr operon4. Δnqr cells containing the wild-type or mutant nqr operon on a pBAD plasmid were grown in Luria-Bertani (Miller) medium in 30-liter fermenters (New Brunswick BF-5000, Microbiology Core Facilty, CBIS, RPI) at 37 °C with constant agitation (300 rpm) and aeration of 20 L/min. Expression of the nqr operon was induced by arabinose. Cells were harvested, washed by centrifugation, and disrupted by passing through a Microfluidizer at a pressure of 20,000 p.s.i32. The membrane preparations were obtained by ultracentrifugation (100,000 g) and washed with a buffer containing 50 mM NaPi, 5.0 mM imidazole, 300 mM NaCl, and 0.05% glycerol. The site-directed mutant NqrB-Glu157Ala was obtained using the QuickChange II XL mutagenesis kit (Agilent), as reported before32. The forward primer was 5’-CGCAAGCATGAAGTCAACGCTGGTTTCTTCGTTACCTCTAT-3’ and the reverse primer was complementary to the forward primer.
Purification of Na+-NQR
The membrane pellets (40 g) were solubilized in 600 mL of solubilization buffer [50 mM NaPi, 300 mM NaCl, 5.0 mM imidazole, and protease inhibitor cocktail (Sigma-Aldrich), pH 8.0] containing 0.4% (w/v) n-dodecyl-β-D-maltoside (DDM) with stirring for 1 h at 4°C4. The mixture was clarified by ultracentrifugation (100,000 g), and supernatant was mixed with 20 mL of Ni-NTA resin (Qiagen) that had been equilibrated with binding buffer (50 mM NaPi, 300 mM NaCl, 5.0 mM imidazole, and 0.05% DDM). After gentle agitation for 1 h at 4˚C, the suspension was poured into an empty plastic column. The column was washed with 4 volumes of binding buffer, followed by 5 volumes of wash buffer (same as binding buffer but containing 10 mM imidazole). The protein was eluted from the column using elution buffer (same as binding buffer but containing 100 mM imidazole).
The resulting Na+-NQR preparation was applied to a DEAE anion exchange column (HiPrep DEAE FF 16/10, connected to an ÄKTA system, Cytiva) equilibrated with buffer A [50 mM Tris/HCl, 1.0 mM EDTA, 5% (v/v) glycerol, and 0.05% (w/v) DDM, pH 8.0]. The protein was eluted with a linear gradient of buffer B (buffer A containing 2.0 M NaCl), and the fractions containing pure Na+-NQR were pooled and concentrated at 15~20 mg of protein/mL. Subunit assembly was verified by SDS-PAGE33 and enzyme activity was checked by the inhibitor-sensitive NADH-UQ1 oxidoreductase activity measurements at 282 nm (ε = 14.5 mM−1cm−1,18).
For the preparation of EM grids, an aliquot of this enzyme preparation (50 µL, 780 µg) was further purified by chromatography on a Superose™ 6 Increase 10/300 gel filtration column (Cytiva), equilibrated with buffer (50 mM Tris-HCl, 100 mM NaCl, 1.0 mM EDTA, 0.05% DDM, pH 8.0) to remove glycerol from the sample. The peak fraction containing Na+-NQR (3.0 mL) was concentrated to 20 µL using an Amicon Ultra 100 K centrifugal filter (Merck-Millipore) and used immediately for the cryo-EM grid preparation.
Cryo-EM acquisition
To prepare the cryo-EM grid for purified Na+-NQR, 2.7 μL of the enzyme solution (50 µM) was loaded onto a glow-discharged Quantifoil Cu R1.2/1.3 and blotted by Vitrobot IV followed by vitrification with liquid ethane. To obtain samples with bound aurachin D-42 (abbreviate as “Na+-NQRAD42”) or korormicin A (abbreviate as “Na+-NQRKA”), the enzyme and inhibitor were mixed at a 1:10 molar ratio and incubated for 10 seconds before loading on a grid. Cryo-EM movies were automatically acquired using a Titan Krios electron microscopy (Thermo Fisher, USA) equipped with K3 BioQuantum camera (Gatan, United States) using SerialEM software34. The cryo-EM movies were collected at a nominal magnification of 81,000 and the pixel size was 0.88 Å/pix. The total electron dose and frame rate were 65 electrons/Å2 and 0.1 s, respectively.
Image processing
Image processing steps for each condition are summarized in Supplementary Fig. 2. All steps were performed by cryoSPARC v3.2.0 or v3.3.1 software35. Topaz software was used for machine-learning-based particle picking36. For native Na+-NQR, Na+-NQRAD42, and Na+-NQRKA, 6,336, 11,430, and 10,406 movies were used, respectively. After motion correction and constant transfer function (CTF estimation), auto-picking based on Topaz machine-learning resulted in 634,154, 3,350.484, and 721,916 particles for Na+-NQR, Na+-NQRAD42, and Na+-NQRKA, respectively. Particles were extracted from the motion-corrected micrographs and selected using Heterogenous Refinement. The selected particles were subjected to Non-uniform Refinement with the optimizing per-particle defocus option. The refinement provided density maps for Na+-NQR, Na+-NQRAD42, and Na+-NQRKA at 2.7-, 2.5-, and 2.6-Å resolution, respectively.
The density corresponding to the cytoplasmic domain of NqrF was weak and blurry due to its flexibility. To obtain the structure of this domain, local refinement on NqrA and NqrB was carried out after Non-uniform Refinement. Then, focused 3D classification on NqrF and NqrA without alignment was applied to the results of local refinement. Of the results of focused 3D classification, the particles belonging to the density maps, in which NqrF was clearly observed, were selected: three classes for Na+-NQR (Class 1; 82,790 particles, Class 2; 80,882 particles, Class 3; 72,234 particles), one class for Na+-NQRAD42 (55,375 particles), and one class for Na+-NQRKA (50,444 particles). The selected particles were subjected to Non-uniform Refinement. As a result of these procedures, three density maps of Na+-NQR without bound inhibitor, in which the soluble domain of NqrF was clearly defined, were obtained, all at 3.1-Å resolution. For Na+-NQRAD42 and Na+-NQRKA, density maps at 3.0-Å and 2.9-Å resolution, respectively, were obtained. The resolution was based on the gold standard criterium: Fourier shell correlation = 0.142 criterion.
Model building and refinement
To build the atomic model of Na+-NQR, each subunit modeled in the crystallographic structure (PDBID: 4P6V) was fitted into the density map as a rigid body. The initial model was flexibly fitted against the map using the Chain Refine function of COOT software37. Then, the roughly fixed model was extensively manually corrected, residue by residue, using the Real Space Refine Zone function in COOT. The manually corrected model was refined using the phenix_real_space_refine program38 with secondary structure and Ramachandran restraints. The resulting model was manually checked by COOT. The water molecules were placed in the models manually. This iterative process was performed for several rounds to correct remaining errors until the model satisfied the geometry. The model quality was assessed by Molplobity and EMRinger scores39,40. For model validation against over-fitting, the final models were used to calculate Fourier shell correlation against the final density maps employed in model building using the phenix refine the program. The statistics of maps and models presented here are summarized in Supplementary Tables 1 and 2. All figures were prepared using UCSF chimeraX software41. Electrostatic potential was calculated by UCSF ChimeraX, using Coulombic command with a range between −10 and 10 kcal/(mol·e) and default settings.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The cryo-EM maps have been deposited in the EMDB under accession codes, 33242 (Na+-NQR, state 1), 33243 (Na+-NQR, state 2), 33244 (Na+-NQR, state 3), 33245 (Na+-NQR, with AD-42), and 33246 (Na+-NQR, with korormicin A). The consensus maps for Na+-NQR, Na+-NQRAD42, and Na+-NQRKA were also deposited as additional maps in the depositions, 33242, 33245, and 33246. The atomic models have been deposited in the Protein Data Bank under accession codes, 7XK3 (Na+-NQR, state 1), 7XK4 (Na+-NQR, state 2), 7XK5 (Na+-NQR, state 3), 7XK6 (Na+-NQR, with AD-42), and 7XK7 [https://doi.org/10.2210/pdb7XK3/pdb] (Na+-NQR, with korormicin A). The initial model for model building is accessible in PDB under accession number 4P6V (crystal structure of Na+ -NQR reported by Steuber et al.16). The data that support the findings of this study are available from the corresponding author upon reasonable request. Source data (for Supplementary Fig. 4) are provided with this paper. Source data are provided with this paper.
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Acknowledgements
We thank Dr. Joel E. Morgan (Rensselaer Polytechnic Institute) for the helpful discussion. This work was supported by JSPS KAKENHI (Grant Numbers JP21H02130 to H.M., 22K14837 to T.M., 20K06514 to J.K., 22J10185 to M.I. and 22H02273 to M.M.), and the National Institutes of Health (RO1-AI132580 to B.B.). N.L.B was supported by NIH-NAA training grant (T32AG057464).
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J.K., M.I., T.M., M.M., B.B., and H.M. designed the research; M.I., T.M., N.L.B., and B.B. expressed and purified Na+-NQR; J.K., M.I., and T.M. performed EM sample preparation and data collection. J.K., M.I., and T.K. performed structural determination and built the atomic models. Y.K. and M.I. analyzed the equilibrium model. J.K., M.I., T.M., M.M., B.B., and H.M. analyzed data; H.M. directed the project and wrote the paper with J.K., I.M., T.M., M.M., and B.B.
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Kishikawa, Ji., Ishikawa, M., Masuya, T. et al. Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae. Nat Commun 13, 4082 (2022). https://doi.org/10.1038/s41467-022-31718-1
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DOI: https://doi.org/10.1038/s41467-022-31718-1
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