Abstract
Leishmaniasis is one of the top neglected tropical diseases with significant morbidity and mortality in low and middle-income countries (LMIC). However, this disease is also spreading in the developed world. Currently, there is a lack of effective strategies to control this disease. Vaccination can be an effective measure to control leishmaniasis and has the potential to achieve disease elimination. Recently, we have generated centrin gene-deleted new world L. mexicana (LmexCen−/−) parasites using CRISPR/Cas9 and showed that they protect mice against a homologous L. mexicana infection that causes cutaneous disease. In this study, we tested whether LmexCen−/− parasites can also protect against visceral leishmaniasis caused by L. donovani in a hamster model. We showed that immunization with LmexCen−/− parasites is safe and does not cause lesions. Furthermore, such immunization conferred protection against visceral leishmaniasis caused by a needle-initiated L. donovani challenge, as indicated by a significant reduction in the parasite burdens in the spleen and liver as well as reduced mortality. Similar control of parasite burden was also observed against a sand fly mediated L. donovani challenge. Importantly, immunization with LmexCen−/− down-regulated the disease promoting cytokines IL-10 and IL-4 and increased pro-inflammatory cytokine IFN-γ resulting in higher IFN-γ/IL-10 and IFN-γ/IL4 ratios compared to non-immunized animals. LmexCen−/− immunization also resulted in long-lasting protection against L. donovani infection. Taken together, our study demonstrates that immunization with LmexCen−/− parasites is safe and efficacious against the Old World visceral leishmaniasis.
Similar content being viewed by others
Introduction
Leishmaniases are vector-borne parasitic diseases affecting millions of people globally, which clinically range from self-healing cutaneous (CL) to systemic and often fatal visceral leishmaniasis (VL)1,2. The various clinical forms of leishmaniases are caused by different parasite species, and CL is caused by the infections with L. major or L. tropica in the Old World and with L. mexicana species complex in the New World. The more severe and life-threatening VL is caused by the L. donovani complex in the Old World and by L. infantum which is prevalent in the Mediterranean, as well as the New World3. Cutaneous leishmaniasis is endemic in the United States, and presence of sand fly vector may cause further spreading of this disease4,5.
Since most of the chemotherapies against leishmaniasis suffer from limitations like toxicity, high cost, the necessity for long-term use and most importantly emergence of drug resistance6,7,8, vaccination could be effective in achieving control and elimination of leishmaniasis. Recovery from a primary Leishmania infection gives lifelong protection from future infections, thus leading to the insight that vaccination is feasible against leishmaniasis9,10,11,12. However, there is no licensed vaccine available for human use against any form of leishmaniasis.
Earlier studies have shown that deliberate infections with a low dose of virulent live wild-type dermotropic L. major parasites confers protection against reinfection, a process termed leishmanization13,14,15. Leishmanization also afforded cross-protection against VL in various animal models and probably in humans10,16,17. However, such method of immunization is not practical because of the safety concerns regarding skin lesions that may last for months at the site of inoculation in a naïve population18,19. In contrast, immunization with live attenuated dermotropic Leishmania parasites which are non-pathogenic and provide a complete array of antigens like their wild-type analogs, could be a promising vaccine candidate against both CL and VL. While leishmanization with the Old World species of L. major is widely studied to identify the mediators of protective immunity and used as a standard to replicate in numerous experimental vaccine studies, there is no equivalent leishmanization with the New World species of Leishmania, such as L. mexicana. Infections with L. mexicana species cause a more chronic pathology that, unlike skin lesions caused by L. major infection, does not self-resolve. Further, infection with L. mexicana presents clinical features and pathologies distinct from the infections of Old World Leishmania species. For example, the localized cutaneous lesions caused by L. mexicana infection, can progress into diffuse lesions in other parts of the body away with no delayed-type hypersensitivity (DTH) response20,21. In addition, induction of a Th2 dominant response is responsible for pathogenesis in L. mexicana infection of mice22. A similar dominant Th2 response has been shown to play an important role in the pathogenesis of a related New World species, L. braziliensis, in a hamster model23. Therefore, it is necessary to develop vaccines that are effective at preventing infections with the New World Leishmania species. We recently demonstrated the safety and efficacy of a marker-free centrin gene-deleted live attenuated new world dermotropic L. mexicana (LmexCen−/−) parasite in a mouse model of New World cutaneous leishmaniasis24. Centrin is a calcium-binding protein, essential in the duplication of centrosomes in eukaryotes including Leishmania25,26. Centrin gene-deficient Leishmania parasites are replication-deficient only in the intracellular amastigote stage but can be easily grown in promastigote culture25. Similar deletion of centrin in L. major (LmCen−/−) showed that immunization with this strain derived from the Old World species of Leishmania can protect against CL and VL challenge infections27,28.
The current study examined whether a New World dermotropic LmexCen−/− parasite is similarly effective against fatal VL in a hamster model. Hamsters develop clinicopathological symptoms of VL similar to human VL, including succumbing to death and are considered a gold standard model of VL29. Data showed that immunization of hamsters with live attenuated LmexCen−/− is safe and cross-protects against a Leishmania donovani challenge infection. Moreover, LmexCen−/− vaccinated hamsters showed downregulation of disease promoting Th2 type of response as indicated by reduced IL-10 and IL-4 cytokine expression and higher IFN-γ response resulting in higher IFN-γ/IL-10 and IFN-γ/IL-4 ratios, the key biomarkers of protection compared to virulent LmexWT infection. Further, immunization with LmexCen−/− resulted in long-lasting protection against L. donovani infection through needle injection. These studies show that immunization with genetically modified New World Leishmania mexicana parasites has the potential as a vaccine against the New World Leishmania species L. mexicana24 and also against the Old World visceral leishmaniasis caused by L. donovani.
Results
LmexCen −/− parasites do not cause any lesion development in hamsters
To evaluate the non-pathogenicity of LmexCen−/− parasites, hamsters were injected intradermally with 106 LmexCen−/− promastigotes. Hamsters were injected with wild-type L. mexicana (106, LmexWT) parasites were used as a control group. Lesion development was monitored up to 11-weeks post-injection, and parasite loads were determined at this study endpoint through the serial dilution method (Fig. 1a). The hamsters were injected with LmexCen−/− parasites did not develop any visible lesions up to 11-weeks of post-injection (Fig. 1b, c). In contrast, the hamsters injected with LmexWT parasites developed ear lesions within 5-weeks of injection that progressively increased in size (Fig. 1b, c). The LmexWT-injected hamsters had significantly higher parasite loads both in the ears (~106 Fig. 1d) and in the dLNs (~104 Fig. 1e) compared to low levels of viable parasites were recovered from the ears (2 out of 7) and draining lymph nodes (1 of 7) of (Fig. 1d, e) LmexCen−/− immunized hamsters. No viable parasites were recovered from the spleen, liver, or bone marrow of either LmexCen−/− or LmexWT injected hamsters at 11-weeks post-inoculation). These results showed that LmexCen−/− parasites are non-pathogenic, thus safe.
Immunization with LmexCen −/− induces a pro-inflammatory immune response
Next, we evaluated the immune response associated with the immunization by analyzing the expression of major proinflammatory (IFN-γ) and disease promoting (IL-10 and IL-4) cytokines in the splenocytes of LmexCen−/− and LmexWT injected hamsters at 11 weeks post-infection (Fig. 2). To measure antigen specific immune response, splenocytes were either unstimulated or stimulated with L. mexicana freeze thawed antigen (±FTAg). In both LmexCen−/− immunized and LmexWT infected animals, upon antigen stimulation, expression of IFN-γ increased in the spleen even though the increase was not statistically significant between the two groups (Fig. 2a). Of note, the parasite burden was significantly different between the two groups (Fig. 1 from parasite burden). We also measured other inflammatory cytokines TNF-α and IL-12p40 at this time point (Fig. 2b, c). Our data indicate no significant difference in their expression between LmexWT and LmexCen−/− immunized groups. Measurement of anti- disease promoting cytokines IL-10 and IL-4, on the other hand, revealed contrasting results. In the spleens of LmexCen−/− immunized hamsters both the IL-10 and IL-4 expression was significantly inhibited compared to LmexWT infected group (Fig. 2d, e). Moreover, the ratios of IFN-γ to IL-10 as well as IFN-γ to IL-4 were significantly higher in the spleens of the LmexCen−/− immunized group compared to LmexWT injected group(Fig. 2f, g), suggesting that LmexCen−/− immunization induces a pro-inflammatory environment while suppressing anti-inflammatory response in the spleens of immunized animals. Collectively, these results demonstrated that the live attenuated LmexCen−/− parasites are immunogenic in a hamster model without causing cutaneous lesions.
Immunization with LmexCen −/− protects against challenge infection with L. donovani through needle and sand fly mediated infection in a hamster model
Since LmexCen−/− parasites are immunogenic in hamsters, we investigated the efficacy of LmexCen−/− immunization against challenge infection with L. donovani. The hamsters were intradermally immunized with LmexCen−/− parasites. Immunized hamsters were challenged at 11-weeks post-immunization with virulent L. donovani wild-type (LdWT) parasites and monitored up to 15-months post-challenge (Fig. 3a). Survival data revealed that LmexCen−/− immunization protected 80% of animals against mortality associated with L. donovani infection, as demonstrated by the survival of 4 out of the 5 animals that remained healthy up to 15-months post-challenge, the endpoint of our study. All the age-matched non-immunized- hamsters died with symptoms characteristic of human VL starting from 9-months post-challenge timepoint and up to 15-months corresponding to the endpoint of the study (Fig. 3b). Spleens isolated from the moribund hamsters from the non-immunized challenged group demonstrated splenomegaly (Fig. 3c). In contrast, spleens from LmexCen−/− immunized challenged group showed no splenomegaly consistent with a lack of pathology in this group. Moreover, analysis of the parasite burden revealed significant control of parasite burden both in the spleen (~5 log reduction, Fig. 3d) and liver tissues (~4 log reduction, Fig. 3e) of LmexCen−/− immunized hamsters compared to the nonimmunized-challenged group. Of note, 60% (3 out of 5) of the livers from immunized animals had no detectable parasites as measured by serial dilution method.
To confirm if immunization with LmexCen−/− can similarly protect the hamsters against a more rigorous challenge with a sand fly mediated L. donovani infection, immunized hamsters were exposed to L. donovani infected-sand flies at 11-weeks post-immunization and monitored for 12-months post-challenge (Fig. 4a). The animals were sacrificed at 10 to12-months, and parasite burden in spleens and livers was determined. There was a significant reduction of parasite burden in both spleen and liver of the immunized hamsters compared to non-immunized animals (~4 log reduction Fig. 4b, c). Together, these data demonstrate that LmexCen−/− immunization protects against both needle and sand fly mediated infection in a hamster model of VL, suggesting that immunization with the New World CL vaccine can also protect against the Old World VL.
Immunization with LmexCen −/− confers long-term protection against fatal visceral infection in a hamster model
Next, to determine the durability of protection induced by LmexCen−/− immunization, hamsters were challenged with the virulent wild-type L. donovani through a needle injection 8-months post-immunization (Fig. 5a). Since we observed comparable protection to both the needle and the sand fly mediated challenges (Figs. 3 and 4), in this experiment only needle challenged was used. Age-matched naïve L. donovani infected animals were used as a control group (non-immune challenged group). Analysis of spleen and liver parasite loads after 8-months post-challenge showed significant control of spleen and liver parasite burden. The immunized hamsters showed lack of splenomegaly (Fig. 5b) and a ∼2 and a ∼1.5 log-fold reduction in the spleen (Fig. 5c) and liver (Fig. 5d) parasite burden, respectively, compared to non-immunized challenged hamsters. These data confirm the long-lasting protective immunity of LmexCen−/− parasites against visceral infection in the preclinical hamster model.
Discussion
Antileishmanial treatment is complex and shows toxic side effects. Therefore, a vaccine is urgently needed against human leishmaniasis, VL in particular. Epidemiological evidence and laboratory studies suggested that infection with one species of Leishmania species can cross-protect against other species10,16,17,30. Particularly, recovery from infection with the Old World species such as L. major parasites has been shown to confer protection against New World species L. infantum, which causes fatal visceral leishmaniasis, confirming that leishmanization is a viable vaccination approach16. Although, infection with Leishmania mexicana induces skin pathology similar to L. major infection, may also result in the development of non-healing lesions in contrast to the spontaneously healing lesions observed in L. major infections20,21. In addition, L. mexicana infection is associated with a dominant Th2 response as compared to L. major parasites22, whereas the role of Th2 response in the pathogenesis caused by L. major is debatable31,32. Therefore, due to the differences in clinical severity and in the immunological mechanisms of pathogenesis, it may be important to develop a vaccine that can protect against infections of Leishmania species prevalent in the New World. Towards that goal, we have developed centrin gene-deleted L. mexicana parasites using the CRISPR/Cas9 technique. Our studies in mouse infection models indicated that LmexCen−/− immunization confers protection against homologous wild-type L. mexicana challenge infection24. Since both the CL and the VL are prevalent in the Americas, a vaccine against these diseases will be needed. Therefore, we wanted to test the hypothesis whether a New World strain derived LmexCen−/− can also protect against a VL infection.
This study reports that LmexCen−/− parasites induce significant host immune response and protect hamsters from developing severe VL disease upon challenge with the Old World L. donovani parasites. Our results show that LmexCen−/− parasites do not cause any pathology in susceptible hosts such as hamsters33. Our data further confirmed the safety characteristics of LmexCen−/− parasites in the hamster model, as was previously shown in mouse models of infection24. Immunization with LmexCen−/− showed downregulation of the disease promoting cytokine response in hamster spleens compared to the spleens of LmexWT-infected animals, as was also observed in mouse models of infection24. Specifically, while LmexWT inoculated hamsters developed a disease promoting cytokine response, as evident by the elevated IL-10 and IL-4 expression levels, LmexCen−/− immunized hamsters displayed a diminished expression of both these cytokines relative to LmexWT infection. The hamster is considered as a gold standard model to study visceral leishmaniasis because it elicits all the clinical features of human disease as well as the expression profile of different cytokines related to disease progression or control were identified. Like humans, in hamsters IL-10 helps in disease progression and corelates with VL, while expression of IL-4 is unaltered34. However, it is well documented that IL-4 levels were high in active VL patients35,36. On the other hand, there was similar induction of pro-inflammatory cytokine IFN-γ, ΤΝF−α and IL−12 between LmexWT infected and LmexCen−/− immunized hamster spleens despite the significant difference in parasite burden in these groups. Overall, analysis of splenic immune response showed the ratios between IFN-γ/IL-10 and IFN-γ/ IL-4 higher in LmexCen−/− immunized hamsters than in LmexWT infection. In mice infected with L. major, IL-4 can play a critical role in modulating the establishment of a protective Th1 response31,37. However, in L. mexicana infection, both IL-10 and IL-4 play a crucial role in disease susceptibility in mouse models38,39. In contrast, LmCen−/− immunization induced a dominant Th1 response (60 folds high IFN-γ compared to LmWT) evident throughout the immunization period27. However, IL-10 and IL-4 responses were significantly diminished in LmexCen−/− immunization (6 folds enrichment of IFN-γ/IL-10 in LmCen−/− versus 2-3 folds enrichment in LmexCen−/−), indicating that LmCen−/− induces an exaggerated immune response when tested at an equivalent timepoint post-immunization in hamsters. It is also well established that in L. mexicana infection, a diminished expression of Th2 response is crucial to provide host protection40,41. Our current studies suggest that immunization with the LmexCen−/− parasites in both rodent models induced a similar protective immune response24.
Next, we observed that the immune response generated by LmexCen−/− immunization induced significant host protection against L. donovani challenge both in the spleen and liver up to 15- months of post-challenge, as evident by the substantial reduction in parasite burden compared to non-immunized animals. In contrast, all non-immunized challenged hamsters developed severe symptoms of VL and succumbed to death by 15-months of post-challenge. On the contrary, 80% of immunized and challenged animals survived until the study ended. In addition, LmexCen−/− induced immunity was long-lasting, as evidenced by the parasite control in animals immunized over eight months compared to non-immunized animals. This could be due to the persistence of vaccine parasites in immunized animals that may play an essential role in maintaining a protective immune response. In support of that, we observed a small number of live vaccine parasites recovered from some of the hamsters even after 11-weeks post-immunization (Fig. 1) that could maintain immunity as was observed in our previous studies with the LmCen−/− parasites27.
In conclusion, we have demonstrated that using CRISPR/Cas9 mediated centrin gene deletion of L. mexicana parasite, a New World Leishmania species, is safe and effective against VL caused by the Old World species, L. donovani. Furthermore, these studies demonstrate that centrin deleted parasites, whether from the Old World or the New World, Leishmania offer an expanded number of vaccine candidates that could be developed to control leishmaniasis in all endemic regions of the world and eventually eliminate the disease. However, there are challenges with the Leishmania vaccines with respect to moving it from bench to bed side in resource poor countries that are Leishmania endemic as was pointed out by Parkash et al.42. We are currently working on some of such challenges.
Methods
Study design and ethical statement
Immunization and challenge infections were performed in a hamster model of VL to determine the efficacy of centrin gene-deleted L. mexicana (LmexCen−/−) parasites as a vaccine against experimental L. donovani infection. All animal experiments in this study were reviewed and approved by the Animal Care and Use Committee of the Center for Biologics Evaluation and Research, U.S. Food and Drug Administration (ASP 1999#23) and the National Institute of Allergy and Infectious Diseases (NIAID) (http://grants.nih.gov/grants/olaw/references/phspolicylabanimals.pdf) under animal protocol LMVR4E. The NIAID DIR Animal Care and Use Program complies with the Guide for the Care and Use of Laboratory Animals and with the NIH Office of Animal Care and Use and Animal Research Advisory Committee guidelines. The housing condition of animals were followed standard guidelines by NIH guidelines for the humane care and use of animals. The body weight, fur and physical appearance was monitored and periodically assessed by the facility veterinarian to assess the development of VL. Sample sizes were calculated as the following: 4 hamsters per group can provide over 80% power to detect a 2-fold difference on parasite load (survival) averaged across the tested time points comparing the hamsters with and without the immunization (α = 0.05 and CV = 30%).
Animals and parasites
Six to eight-week-old female outbred Syrian golden hamsters (Mesocricetus auratus) were obtained from the Harlan Laboratories Indianapolis, USA. All animals were housed either at the Food and Drug Administration (FDA) animal facility, Silver Spring (MD) or the National Institute of Allergy and Infectious Diseases (NIAID), Twin-brook campus animal facility, Rockville (MD), under pathogen-free conditions. The wild-type L. donovani (LdWT) (MHOM/SD/62/1S) parasites, wild-type Leishmania mexicana (MNYC/B2/62/m379) parasites (LmexWT) and centrin gene-deleted LmexCen−/− promastigotes were cultured in liquid M199 culture medium supplemented with 10% fetal bovine serum (FBS), 1% Penicillin/Streptomycin and 1% HEPES at 26 C°24,27.
Immunization of hamsters and determination of parasite load
Six to eight-week-old female hamsters were immunized with 106 total stationary-phase LmexCen−/− parasites by intradermal injection in the left ear in 10 μl PBS using a 29-gauge needle (BD Ultra-Fine). In addition, the age-matched control group of animals were infected with 106 total stationary-phase L. mexicana wild-type (LmexWT) promastigotes. Lesion size was monitored weekly by measuring the diameter of the ear lesion using a direct reading vernier calliper. Parasite burdens in the ear, draining lymph node (dLN), spleen, liver and bone marrow tissues were determined by limiting dilution assay27. Ear tissues were treated with Liberase enzyme (Sigma-Aldrich) (0.15 mg/ml) in DMEM mediun at 37 °C. After 90 min of incubation tissues were grinded to make single cell suspensison using BD medimachine system. Cells were washed two times with M199 medium. Lymph nodes, spleen, liver and bone marrow were harvested and homogenized with a cell strainer in 2 ml of M199 medium supplemented with 10% FBS and 1% Penicillin/Streptomycin. To determine parasite burden cell suspension from each organs were serially diluted in 96 well tissue culture plates. After 12 days of incubation at 26 °C, plates were examined with a inverted microscope and parasite burden were calculated for each organ.
Determination of cytokine expression in splenocytes by real-time PCR
Single cell suspensions from the spleens of hamsters were made at eleven weeks post-immunization with LmexCen−/− as well as infected with LmexWT. The spleen cell suspensions were stimulated with L. mexicana freeze-thawed antigen (FTAg), and total RNA was extracted using PureLink RNA Mini kit (Ambion) at 16 h after restimulation. Aliquots (400 ng) of total RNA were reverse transcribed into cDNA by a high-capacity cDNA reverse transcription kit using random hexamers (Applied Biosystems). Cytokine gene expression levels were determined by TaqMan PCR probe (TaqMan, Universal PCR Master Mix, Applied Biosystems) using a CFX96 Touch Real-Time System (BioRad, Hercules, CA). The sequences of the primers (forward and reverse) and probes (5′ 6-FAM and 3′ TAMRA Quencher) were used to detect the gene expression, Table 127. The data were analyzed with CFX Manager Software. The expression levels of genes of interest were determined by the 2−ΔΔCt method; samples were normalized to γ-actin expression and determined relative to expression values from naive hamsters.
LmexCen −/− Immunization and challenge with needle infection
Six to eight-week-old female hamsters were immunized with 106 total stationary-phase LmexCen−/−parasites by intradermal injection in the left ear in 10 μl PBS using a 29-gauge needle (BD Ultra-Fine). Eleven weeks post-immunization, animals were challenged with a needle injection (ID route) with virulent 5 × 105 metacyclic L. donovani (Ld1S) promastigotes into the contralateral ear. Age-matched non-immunized hamsters were similarly challenged and used as a control group. Hamsters were monitored daily, and after various periods of post-challenge (9- and 15-month post-challenge), animals were sacrificed, and the parasite load in organs (spleen and liver) was measured by limiting dilution assay27.
LmexCen −/− Immunization and challenge with sand fly mediated infection
Female 2–4 days-old Lutzomyia longipalpis sand flies were infected with L. donovani amastigotes (freshly isolated from a terminally sick hamster due to visceral leishmaniasis). The sand flies were infected by artificial feeding through a chicken skin membrane on rabbit blood containing Leishmania donovani amastigotes. Fully blood fed female sand flies were separated and maintained at 26 °C with 75% humidity and were provided 30% sucrose solution. The flies were dissected periodically to assess the the maturity of infection27. Parasite loads and percentage of metacyclic per midgut were determined using hemocytometer counts. Thirty sand flies on day 15- after infection were applied to the ear of each LmexCen−/− immunized and age-matched non-immunized hamsters used for subsequent transmission as described previously. The number of blood-fed flies was determined after transmission as a qualitative measurement. Each hamster received an average of 18 infected bites per transmission. Due to COVID related restrictions it was not possible to have separate control groups for LmCen−/− and LmexCen−/− studies that were conducted concurrently. Thus, the sand fly challenge experiments with non-immunized hamster controls shown in the current study were performed alongside our previous studies on LmCen−/− parasites27. Hamsters were monitored daily during infection, and after 9–15-months of post-challenge, animals were sacrificed, and parasite load in organs (spleen and liver) was measured by limiting dilution assay27.
Statistical analysis
Statistical analysis of differences between groups was determined by unpaired two-tailed Mann–Whitney t-test using Graph Pad Prism 7.0 software.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The data that support the findings of this study are available from the corresponding authors HLN or RD upon request.
References
Burza, S., Croft, S. L. & Boelaert, M. Leishmaniasis. Lancet 392, 951–970 (2018).
Kaye, P. & Scott, P. Leishmaniasis: complexity at the host-pathogen interface. Nat. Rev. Microbiol 9, 604–615 (2011).
McGwire, B. S. & Satoskar, A. R. Leishmaniasis: clinical syndromes and treatment. QJM 107, 7–14 (2014).
Curtin, J. M. & Aronson, N. E. Leishmaniasis in the United States: emerging issues in a region of low endemicity. Microorganisms 9, https://doi.org/10.3390/microorganisms9030578 (2021).
McIlwee, B. E., Weis, S. E. & Hosler, G. A. Incidence of endemic human cutaneous leishmaniasis in the United States. JAMA Dermatol. 154, 1032–1039 (2018).
Taslimi, Y., Zahedifard, F. & Rafati, S. Leishmaniasis and various immunotherapeutic approaches. Parasitology 145, 497–507 (2018).
Ghorbani, M. & Farhoudi, R. Leishmaniasis in humans: drug or vaccine therapy. Drug Des. Devel Ther. 12, 25–40 (2018).
Ponte-Sucre, A. et al. Drug resistance and treatment failure in leishmaniasis: a 21st century challenge. PLoS Negl. Trop. Dis. 11, e0006052 (2017).
Lainson, R. & Shaw, J. J. Leishmaniasis in Brazil: XII. Observations on cross-immunity in monkeys and man infected with Leishmania mexicana mexicana, L. m. amazonensis, L. braziliensis braziliensis, L. b. guyanensis and L. b. panamensis. J. Trop. Med. Hyg. 80, 29–35 (1977).
Porrozzi, R., Teva, A., Amaral, V. F., Santos da Costa, M. V. & Grimaldi, G. Jr. Cross-immunity experiments between different species or strains of Leishmania in rhesus macaques (Macaca mulatta). Am. J. Trop. Med Hyg. 71, 297–305 (2004).
Ostyn, B. et al. Incidence of symptomatic and asymptomatic Leishmania donovani infections in high-endemic foci in India and Nepal: a prospective study. PLoS Negl. Trop. Dis. 5, e1284 (2011).
Jeronimo, S. M. et al. Natural history of Leishmania (Leishmania) chagasi infection in Northeastern Brazil: long-term follow-up. Clin. Infect. Dis. 30, 608–609 (2000).
Nadim, A., Javadian, E., Tahvildar-Bidruni, G. & Ghorbani, M. Effectiveness of leishmanization in the control of cutaneous leishmaniasis. Bull. Soc. Pathol. Exot. Filiales 76, 377–383 (1983).
Kellina, O. I. Problem and current lines in investigations on the epidemiology of leishmaniasis and its control in the U.S.S.R. Bull. Soc. Pathol. Exot. Filiales 74, 306–318 (1981).
Seyed, N., Peters, N. C. & Rafati, S. Translating observations from leishmanization into non-living vaccines: the potential of dendritic cell-based vaccination strategies against leishmania. Front. Immunol. 9, 1227 (2018).
Romano, A., Doria, N. A., Mendez, J., Sacks, D. L. & Peters, N. C. Cutaneous infection with Leishmania major mediates heterologous protection against visceral infection with Leishmania infantum. J. Immunol. 195, 3816–3827 (2015).
Zijlstra, E. E., el-Hassan, A. M., Ismael, A. & Ghalib, H. W. Endemic kala-azar in eastern Sudan: a longitudinal study on the incidence of clinical and subclinical infection and post-kala-azar dermal leishmaniasis. Am. J. Trop. Med Hyg. 51, 826–836 (1994).
Okwor, I. & Uzonna, J. Vaccines and vaccination strategies against human cutaneous leishmaniasis. Hum. Vaccin 5, 291–301 (2009).
Noazin, S. et al. First generation leishmaniasis vaccines: a review of field efficacy trials. Vaccine 26, 6759–6767 (2008).
Scorza, B. M., Carvalho, E. M. & Wilson, M. E. Cutaneous manifestations of human and murine leishmaniasis. Int J Mol Sci. 18, https://doi.org/10.3390/ijms18061296 (2017).
Gabriel, A. et al. Cutaneous leishmaniasis: the complexity of host’s effective immune response against a polymorphic parasitic disease. J. Immunol. Res 2019, 2603730 (2019).
Rosas, L. E. et al. Genetic background influences immune responses and disease outcome of cutaneous L. mexicana infection in mice. Int Immunol. 17, 1347–1357 (2005).
Paiva, M. B. et al. A cytokine network balance influences the fate of Leishmania (Viannia) braziliensis infection in a cutaneous leishmaniasis hamster model. Front Immunol. 12, 656919 (2021).
Volpedo, G. et al. Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis. NPJ Vaccin. 7, 32 (2022).
Selvapandiyan, A. et al. Centrin gene disruption impairs stage-specific basal body duplication and cell cycle progression in Leishmania. J. Biol. Chem. 279, 25703–25710 (2004).
Selvapandiyan, A. et al. Centrin1 is required for organelle segregation and cytokinesis in Trypanosoma brucei. Mol. Biol. Cell 18, 3290–3301 (2007).
Karmakar, S. et al. Preclinical validation of a live attenuated dermotropic Leishmania vaccine against vector transmitted fatal visceral leishmaniasis. Commun. Biol. 4, 929 (2021).
Zhang, W. W. et al. A second generation leishmanization vaccine with a markerless attenuated Leishmania major strain using CRISPR gene editing. Nat. Commun. 11, 3461 (2020).
Aslan, H. et al. A new model of progressive visceral leishmaniasis in hamsters by natural transmission via bites of vector sand flies. J. Infect. Dis. 207, 1328–1338 (2013).
Dey, R. et al. Characterization of cross-protection by genetically modified live-attenuated Leishmania donovani parasites against Leishmania mexicana. J. Immunol. 193, 3513–3527 (2014).
Biedermann, T. et al. IL-4 instructs TH1 responses and resistance to Leishmania major in susceptible BALB/c mice. Nat. Immunol. 2, 1054–1060 (2001).
Hurdayal, R. et al. Deletion of IL-4 receptor alpha on dendritic cells renders BALB/c mice hypersusceptible to Leishmania major infection. PLoS Pathog. 9, e1003699 (2013).
Wilson, H. R., Dieckmann, B. S. & Childs, G. E. Leishmania braziliensis and Leishmania mexicana: experimental cutaneous infections in golden hamsters. Exp. Parasitol. 47, 270–283 (1979).
Melby, P. C., Chandrasekar, B., Zhao, W. & Coe, J. E. The hamster as a model of human visceral leishmaniasis: progressive disease and impaired generation of nitric oxide in the face of a prominent Th1-like cytokine response. J. Immunol. 166, 1912–1920 (2001).
Carvalho, E. M. et al. Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis. J. Immunol. 152, 5949–5956 (1994).
Karp, C. L. et al. In vivo cytokine profiles in patients with kala-azar. Marked elevation of both interleukin-10 and interferon-gamma. J. Clin. Invest 91, 1644–1648 (1993).
Poudel, B. et al. Acute IL-4 governs pathogenic T cell responses during Leishmania major Infection. Immunohorizons 4, 546–560 (2020).
Padigel, U. M., Alexander, J. & Farrell, J. P. The role of interleukin-10 in susceptibility of BALB/c mice to infection with Leishmania mexicana and Leishmania amazonensis. J. Immunol. 171, 3705–3710 (2003).
Alexander, J. et al. An essential role for IL-13 in maintaining a non-healing response following Leishmania mexicana infection. Eur. J. Immunol. 32, 2923–2933 (2002).
Bryson, K. J. et al. BALB/c mice deficient in CD4 T cell IL-4Ralpha expression control Leishmania mexicana Load although female but not male mice develop a healer phenotype. PLoS Negl. Trop. Dis. 5, e930 (2011).
Satoskar, A., Bluethmann, H. & Alexander, J. Disruption of the murine interleukin-4 gene inhibits disease progression during Leishmania mexicana infection but does not increase control of Leishmania donovani infection. Infect. Immun. 63, 4894–4899 (1995).
Parkash, V., Kaye, P. M., Layton, A. M. & Lacey, C. J. Vaccines against leishmaniasis: using controlled human infection models to accelerate development. Expert Rev. Vaccin. 20, 1407–1418 (2021).
Acknowledgements
Funding was provided from the Global Health Innovative Technology Fund, the Canadian Institutes of Health Research (to GM), intramural funding from CBER, FDA (to HLN), and the Fonds de recherche du Québec – Santé (to PL). This research was supported, in part, by the Intramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases (FO, JO, CM, SK and JGV). The findings of this study are an informal communication and represent the authors’ own best judgments. These comments do not bind or obligate the Food and Drug Administration.
Author information
Authors and Affiliations
Contributions
Su.K., G.V., W.W.Z., P.L., N.I., F.O., J.O., S.G., C.M. and R.D. conducted experiments, analyzed data and helped to write the manuscript. Su.K., R.D., A.R.S., S.H., P.D., S.K., S.S., J.G.V. and H.L.N. designed experiments, analyzed data and wrote the manuscript.
Corresponding authors
Ethics declarations
Competing interests
The FDA is currently a co-owner of two US patents that claim attenuated Leishmania species with the centrin gene deletion (US7,887,812 and US 8,877,213). All other authors declare no competing interests.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Karmakar, S., Volpedo, G., Zhang, WW. et al. Centrin-deficient Leishmania mexicana confers protection against Old World visceral leishmaniasis. npj Vaccines 7, 157 (2022). https://doi.org/10.1038/s41541-022-00574-x
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41541-022-00574-x
- Springer Nature Limited