Abstract
HIV-1 has caused 35 million deaths globally, and approximately the same number is currently living with HIV-1. The trans-activator of transcription (Tat) protein of HIV-1 plays an important regulatory function in the virus life cycle, responsible for regulating the reverse transcription of the viral genome RNA. Tat is found in the nucleus of infected cells, but can also invade uninfected neighbouring cells. Regions within Tat responsible for these cellular localisations are overlapping and include a nuclear localisation signal (NLS) spanning 48GRKKRR, and a cell penetrating peptide (CPP) signal spanning 48GRKKRRQRRRAPQN. However, the mechanism by which this NLS/CPP region mediates interaction with the nuclear import receptors remains to be resolved structurally. Here, we establish that the HIV-1 Tat:NLS/CPP is able to form a stable and direct interaction with the classical nuclear import receptor importin-α and using x-ray crystallography, we have determined the molecular interface and binding determinants to a resolution of 2.0 Å. We show for the first time that the interface is the same as host factors such as Ku70 and Ku80, rather than other virus proteins such as Ebola VP24 that bind on the outer surface of importin-α.
Similar content being viewed by others
Introduction
The HIV-1 virus has spread worldwide, infecting 60 million people, and causing more than 25 million deaths. More than 30 million people currently live with the disease1, but despite highly active antiretroviral therapy (HAART) reducing the effects of the virus, these antivirals do not clear the virus from infected patients. HIV-1 encodes three groups of proteins that are common in all retroviruses. The gag polyprotein, pol polyprotein and gp160 precursors are structural proteins that form the outer shell of the virus particle, and are processed to produce proteins for the virion interior. The accessory regulatory proteins, Vif, Vpr, Vpu and Nef, interact with cellular ligands and function as adapter molecules or to inhibit normal host function. The third group are the essential regulatory elements, Tat and Rev. The primary role of Tat is in regulating the reverse transcription of viral genome RNA, whilst Rev is responsible for the synthesis of major viral proteins for viral replication2.
Tat is a transcriptional trans-activator and plays an important role during HIV-1 replication by binding to a short-stem loop structure, known as the transactivation response element (TAR) located at the 5′ end of HIV RNAs. It assists in the elongation phase of HIV-1 transcription so that full-length transcripts can be produced3, and these functions occur within the nucleus of infected cells. Tat has been shown to localise to the nucleus in many studies, however, the mechanism by which it interacts with the nuclear import receptors has not been elucidated structurally4, 5.
Nuclear import can occur through passive diffusion (<45 kDa) or by energy dependent nuclear import receptors. The classical nuclear import pathway is the best characterised mechanism and is mediated by an adaptor molecule, importin-α, also known as the classical nuclear import receptor, binding cargo that can display a nuclear localisation signal (NLS). The transport carrier importin-β interacts with importin-α, and mediates translocation across the nuclear envelope through interactions with the nucleoporin proteins lining the nuclear pore complex6, 7. Upon entry to the nucleus, the heterotrimer transport complex is dissociated by the small GTPase Ran, releasing the NLS-containing cargo, and allowing recycling of the import receptors back to the cytoplasm8, 9.
The HIV-1 Tat derived cell penetrating peptide (48GRKKRRQRRRAPQN61;CPP) has been shown to effectively carry a large range of cargoes, from nanoparticles, peptides, nucleic acids and even proteins into cells and the nucleus10,11,12,13,14. In vitro studies have shown that Tat is able to bind nuclear import receptors which mediate nuclear localisation5, 15, however, a structural basis for this interaction remains to be elucidated. There has also been some debate in the literature about whether Tat can bind directly to importin-α16 or importin-β15. To determine the precise binding determinants that mediate interaction between the nuclear import receptor and Tat, the entire cell penetrating region of HIV-1 Tat, 48GRKKRRQRRRAPQN61, was recombinantly expressed as a GST-fusion and tested for binding to both importin-α and importin-β6, 16. We found a strong and direct interaction between Tat:NLS/CPP and importin-α, and no direct interaction with importin-β. Together with structural elucidation of the interface by x-ray crystallography, this study provides new insights into the interface between these two proteins which mediate localisation of Tat to the nucleus.
Materials and Methods
Plasmid preparation
Tat residues (48GRKKRRQRRRAPQN61) were codon optimised for expression in E. coli and cloned into the PGEX4T-1 vector at BamHI/EcoRI sites with an additionally engineered N-terminal TEV site for GST-tag cleavage. An isolate of mouse importin-α (homologue of human importin-α; 95% sequence identity) that lacks the auto-inhibitory N-terminal importin-β binding (IBB) domain (residues 70–529) and cloned into the pET30 expression vector has been described previously17. An isolate of mouse importin-β (KPNB1, homologue of human importin-α: 99% sequence identity) was cloned into the pMCSG21 vector using protocols described previously18, 19.
Recombinant Expression and Purification
Overexpression of importin-α and importin-β was performed using the autoinduction method according to Studier20 and purified as outlined previously21. Briefly, cells were resuspended in His buffer A (50 mM phosphate buffer, 300 mM NaCl, 20 mM Imidazole, pH 8), and lysed by two freeze-thaw cycles. The soluble cell extract was injected onto a five mL HisTrap HP column (GE Healthcare) and washed with twenty column volumes of His buffer A on an AKTApurifier FPLC. The sample was eluted using an increasing concentration gradient of imidazole, and eluent fractions were pooled and loaded onto a HiLoad 26/60 Superdex 200 column, pre-equilibrated in buffer A (50 mM Tris pH 8, 125 mM NaCl). Fractions corresponding to the correct molecular weight were collected, and assessed for purity by SDS-PAGE.
Tat-NLS was over-expressed as a GST-fusion protein using IPTG to induce the cells at an OD600 of 0.6. Following growth for 24 h, cells were harvested by centrifugation, at 6,000 rpm for 30 min and the cell pellet resuspended in buffer A and stored at −20 °C. The soluble cell extract containing the GST:Tat-NLS fusion was injected onto a GST 5 mL HP column pre-equilibrated in GST buffer A and washed with GST buffer A until the UV reading stabilised to baseline. The purified importin-α or importin-β was then added to the GST column, washed for a further ten column volumes, and eluted in GST buffer A containing 10 mM glutathione. To cleave the GST affinity tag from the Tat:NLS/CPP importin-α, TEV protease cleavage was undertaken overnight, and continued until cleavage was complete. The protein mixture was further purification by size-exclusion chromatography on a HiLoad 26/60 Superdex 200 column (GE Healthcare), and the complex concentrated to 7 mg/mL using an Amicon MWCO 10 kDa filter, aliquoted, and stored at −80 °C.
Crystallization of Tat-NLS with Importin-α
The final crystallisation condition contained 1.25 M sodium citrate pH 7 and 10 mM DTT. The Tat:NLS/CPP importin-α complex was screened using hanging drop vapour diffusion method where the protein was mixed in a 1:1 ratio with crystallization condition containing reservoir solution and incubated at 296 K. Large rod shaped crystals grew after four days, and the crystals were cryoprotected in a reservoir solution 20% glycerol prior to being flash cooled to 100 K in liquid nitrogen.
Data Collection and Structure Determination
A single crystal was used to collect x-ray diffraction data on the MX2 crystallography beamline at the Australian Synchrotron. The diffraction data was processed using iMosflm22 and scaled and merged using Aimless23, 24. The data was phased by molecular replacement using Phaser25 and Protein Data Bank (PDB) structure 5FC8 as a search model. Model rebuilding and refinement were undertaken in Coot26,27,28 and Phenix29, respectively.
GST-pulldown Assay for Affinity Determination
For determination of the dissociation constant, KD, glutathione agarose beads washed in GST buffer A were saturated with purified GST-Tat:NLS/CPP. Beads were washed three times in GST buffer A and distributed equally in 10 µL aliquots. To each aliquot, 100 µL of two-fold serially diluted importin-α was added, with the initial concentration of 30 µM. One additional tube containing 0 µM importin-α was used as a control. The samples were incubated for one hour at 4 °C before being washed twice with 1 mL of GST buffer A. Binding was assessed by adding 20 µL of tris-glycine sample loading buffer to the beads, boiled for 10 min, and analysed by SDS-PAGE. Images were recorded using BioRad Gel Doc system and processed in ImageJ30. The data was normalised across each replicate experiment and data analysed using one-site specific binding analysis performed in Prism version 7.0b for Mac, GraphPad Software, La Jolla California USA, www.graphpad.com.
Results
The Tat:NLS/CPP region forms a direct interaction with importin-α
The NLS/CPP region of Tat, spanning residues 49–61, have been shown to contain a functional NLS, however, there has been recent debate as to whether the highly basic cell penetrating peptide region is bound using the importin-α adapter, or can bind directly to importin-β. Since this region contains a large stretch of positively charged residues, many of which of which could fit the definition of a classical NLS binding to importin-α, or an Arg rich importin-β interaction, we tested binding against both types of receptors. Here, we immobilised the GST-Tat:NLS/CPP fusion protein onto a glutathione column, washed the column, then passed each respective importin over the immobilised proteins to assess binding. We observed that most of the importin-α was retained on the column (Fig. 1A), whilst little, if any importin-β remained bound (Fig. 1B). These results indicate a direct binding between the Tat:NLS/CPP and the classical nuclear import receptor importin-α.
Protein purification and complex formation
To determine the structural basis for the interaction between the nuclear import receptor importin-α and Tat NLS/CPP, both proteins were purified to homogeneity and isolated as an equimolar complex using the following series of purifications. The nuclear import receptor importin-α was first purified by 6-His affinity and size exclusion chromatography, then loaded on a column containing purified GST-Tat:NLS/CPP. The excess importin-α was removed by washing the column extensively and following elution, the GST affinity tag was removed by proteolytic cleavage with the TEV protease. The mixture was then purified by size exclusion chromatography, where the importin-α:Tat NLS/CPP complex (>58 kDa) was successfully separated from excess Tat NLS/CPP (<5 kDa), resulting in a homogenous equimolar complex for crystallisation.
Protein crystallisation and data collection
The hanging-drop vapour diffusion method was used to obtain large rod-shaped crystals after four days (Fig. 2A). The crystal diffracted to 2.0 Å (Fig. 2B) resolution on the MX2 beam line at the Australian Synchrotron, and a total of 110° of data, collected at 0.5° oscillations, were indexed, merged and scaled using iMosflm v.1.0.722 and AIMLESS23, 24 (Table 1). The structure was solved by molecular replacement using Phaser25, 31 with PDB 5FC8 as a model. Following rebuilding and refinement, the 2.0 Å resolution structure was refined to a Rwork/Rfree of 0.16/0.19 using iterative cycles of refinement and modelling in Phenix29 and COOT28 respectively. The final model consisted of 426 residues of importin-α, 8 residues of the Tat:NLS/CPP, and 319 water molecules. The N- and C-terminal amino acid residues of the Tat:NLS/CPP peptide displayed poor density due to flexibility, allowing only accurate placement of the protein peptide chain. Stereochemistry and other refinement statistics are presented in Table 1.
Binding determinants of the HIV-1 Tat:NLS/CPP in complex with importin-α
The overall structure of importin-α exhibited an all α-helical structure arranged as ten sequential armadillo (ARM) motifs as described previously6. The Tat:NLS/CPP binds to the major binding site of importin-α within ARM domains 2–4 (Fig. 3A,B). There are no NLS residues in the minor binding site, indicating that the NLS region is monopartite. The main chain of Tat residue Arg49 interacts with the side chain of importin-α residue Asn235 in the P1 site (Figs 4 and 5). Tat residue Lys50 binds importin-α at the P2 site where it forms a salt bridge with importin-α residue Asp192 as well as additional side chain interactions with importin-α residues Gly150 and Thr155. The Tat peptide backbone of residue Lys51 hydrogen bonds the side chains of importin-α residues Asn188 and Trp184 in the importin-α P3 binding site. The side-chain of Tat residue Arg52 in the P4 position hydrogen bonds with the importin-α main chain of residues Leu104, Arg106, and Glu107. The P5 binding site is occupied by Tat residue Arg53 which makes main chain interactions with the side chains of importin-α residues Trp142 and Asn146. The overall binding buries 717 Å2 of surface area, and is mediated by 15 hydrogen bonds and 1 salt bridge interaction (Figs 3 and 4). Further details on the NLS binding determinants are shown in Figs 4B and 5 and summarised in Table 2.
Binding affinity of the HIV-1 Tat:NLS/CPP in complex with importin-α
To estimate the binding affinity, importin-α was serially titrated against equal concentrations of HIV-1 Tat:NLS/CPP, and binding captured using a GST-pulldown. The binding affinity was determined to be 1.2 ± 0.2 µM from three replicates (Fig. 6). The binding affinity measured for HIV-1 Tat:NLS/CPP is in the low micromolar range and similar to previously reported values of other NLSs including Dengue 2 C-terminal NS5, 0.27 ± 0.1 µM; and Dengue 3 C-terminal NS5 0.37 ± 0.11 µM32.
Discussion
There has been contention as to which nuclear import receptor is responsible for the nuclear translocation of Tat. One study suggests Tat is importin-α mediated15, whereas another study has shown that it is dependent on importin-β16. Here, we show that the C-terminal 55RRR is not providing additional binding to importin-α, and of the residues visible in the crystal structure 48GRKKRRQR, only residues 48GRKKRR mediate binding with importin-α. Our results support the findings of Ruben et al., who have shown nuclear import can be mediated by Tat-NLS/CPP residues GRKKR16. This binding motif is consistent with the previously defined monopartite class 2 NLSs33. The P1-P5 binding determinants in Tat:NLS/CPP are resolved within the presented 2.0 Å structure, and are consistent with previously solved structures (Table 3 and Fig. 7).
The major binding site of importin-α interact with five principal binding determinants in NLSs, known as P1-P5 (Table 3). These binding determinants P1-P5 bind between ARM domains 2–4 in importin-α and are conserved across previously determined NLS structures (Fig. 7). Position 1 (P1) on an NLS is the most divergent as only a main chain interaction is made with importin- α. Position 2 (P2) is the most highly conserved with a strict requirement of a lysine residue at this position to form the critical salt bridge interaction with importin-α residue D192 as well as side chain interactions with the main chains of importin-α residues T155 and G150. Position 3 (P3), position 4 (P4), and position 5 (P5), prefer long basic amino acids, however, for all characterised NLSs, there is a great variability in the amino acids at these positions (Fig. 7). The P3 site which is less conserved than the P2 site, can bind to Lys, Arg, and Leu residues, whilst the P5 site is similarly conserved and can bind Lys, Arg, and Asn residues. The P4 site is less conserved than P4 and P5, and can bind Pro, Phe, Arg, Lys, Leu, Tyr, and P1 is the least conserved binding to Met, Asn, Lys, Arg, Leu, Ala, and Ser residues32 (Table 3). The Tat:NLS/CPP (Fig. 5A,B) residues 49RKKRR fit within the requirements of the P1-P5 NLSs and bind importin-α in a similar fashion to other importin-α bound NLS structures, whilst other positively charged residues 55RRR were found not to bind in our structure. The similarities between HIV-1 Tat:NLS/CPP and previously reported NLS importin-α structures can be seen in sequence conservation (Table 2), structural overlay comparisons (Fig. 7) and from calculated RMSD values that varied from 0.150–0.431 Å (Table 4). Overall, this is the first time the NLS region within HIV-1 Tat has been determined structurally by X-ray crystallography. Through interaction with the N-terminal arginine rich motifs in importin-α, eight amino acids in Tat have been revealed and binding determinants identified that mediate interaction with the classical nuclear import receptor.
References
Mansueto, P. et al. Vitamin D Deficiency in HIV Infection: Not Only a Bone Disorder. BioMed research international 2015 (2015).
Strebel, K. HIV accessory proteins versus host restriction factors. Current opinion in Virology 3, 692–699, doi:10.1016/j.coviro.2013.08.004 (2013).
Southgate, C. D. & Green, M. R. The HIV-1 Tat protein activates transcription from an upstream DNA-binding site: implications for Tat function. Genes & Development 5, 2496–2507 (1991).
Frankel, A. D. & Pabo, C. O. Cellular uptake of the tat protein from human immunodeficiency virus. Cell 55, 1189–1193, doi:10.1016/0092-8674(88)90263-2 (1988).
Vives, E., Brodin, P. & Lebleu, B. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. Journal of Biological Chemistry 272, 16010–16017, doi:10.1074/jbc.272.25.16010 (1997).
Kobe, B. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin α. Nature Structural & Molecular Biology 6, 388–397, doi:10.1038/7625 (1999).
Fontes, M. R., Teh, T. & Kobe, B. Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-α. Journal of molecular biology 297, 1183–1194, doi:10.1006/jmbi.2000.3642 (2000).
Marfori, M., Lonhienne, T. G., Forwood, J. K. & Kobe, B. Structural Basis of High‐Affinity Nuclear Localization Signal Interactions with Importin‐α. Traffic 13, 532–548, doi:10.1111/j.1600-0854.2012.01329.x (2012).
Cingolani, G., Petosa, C., Weis, K. & Müller, C. W. Structure of importin-β bound to the IBB domain of importin-α. Nature 399, 221–229, doi:10.1038/20367 (1999).
Brooks, H., Lebleu, B. & Vivès, E. Tat peptide-mediated cellular delivery: back to basics. Advanced drug delivery reviews 57, 559–577, doi:10.1016/j.addr.2004.12.001 (2005).
Berry, C. Intracellular delivery of nanoparticles via the HIV-1 tat peptide (2008).
Fawell, S. et al. Tat-mediated delivery of heterologous proteins into cells. Proceedings of the National Academy of Sciences 91, 664–668, doi:10.1073/pnas.91.2.664 (1994).
Eguchi, A. et al. Protein transduction domain of HIV-1 Tat protein promotes efficient delivery of DNA into mammalian cells. Journal of Biological Chemistry 276, 26204–26210, doi:10.1074/jbc.M010625200 (2001).
Rudolph, C. et al. Oligomers of the arginine-rich motif of the HIV-1 TAT protein are capable of transferring plasmid DNA into cells. Journal of Biological Chemistry 278, 11411–11418, doi:10.1074/jbc.M211891200 (2003).
Truant, R. & Cullen, B. R. The arginine-rich domains present in human immunodeficiency virus type 1 Tat and Rev function as direct importin β-dependent nuclear localization signals. Molecular and cellular biology 19, 1210–1217, doi:10.1128/MCB.19.2.1210 (1999).
Ruben, S. et al. Structural and functional characterization of human immunodeficiency virus tat protein. Journal of virology 63, 1–8 (1989).
Teh, T., Tiganis, T. & Kobe, B. Crystallization of importin α, the nuclear-import receptor. Acta Crystallographica Section D: Biological Crystallography 55, 561–563, doi:10.1107/S0907444998012943 (1999).
Eschenfeldt, W. H., Stols, L., Millard, C. S., Joachimiak, A. & Donnelly, M. I. A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins. Methods in molecular biology (Clifton, N.J.) 498, 105–115, doi:10.1007/978-1-59745-196-3_7 (2009).
Nanson, J. D. & Forwood, J. K. Crystallization and preliminary X-ray diffraction analysis of FabG from Yersinia pestis. Acta Crystallographica. Section F, Structural Biology. Communications 70, 101–104, doi:10.1107/S2053230X13033402 (2014).
Studier, F. W. Protein production by auto-induction in high-density shaking cultures. Protein expression and purification 41, 207–234, doi:10.1016/j.pep.2005.01.016 (2005).
Patterson, E. I., Dombrovski, A. K., Swarbrick, C. M., Raidal, S. R. & Forwood, J. K. Structural determination of importin alpha in complex with beak and feather disease virus capsid nuclear localization signal. Biochemical and biophysical research communications 438, 680–685, doi:10.1016/j.bbrc.2013.07.122 (2013).
Battye, T. G. G., Kontogiannis, L., Johnson, O., Powell, H. R. & Leslie, A. G. W. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta Crystallographica Section D: Biological Crystallography 67, 271–281, doi:10.1107/S0907444910048675 (2011).
Evans, P. Scaling and assessment of data quality. Acta Crystallographica Section D: Biological Crystallography 62, 72–82, doi:10.1107/S0907444905036693 (2006).
Evans, P. R. An introduction to data reduction: space-group determination, scaling and intensity statistics. Acta Crystallographica Section D: Biological Crystallography 67, 282–292, doi:10.1107/S090744491003982X (2011).
McCoy, A. J. et al. Phaser crystallographic software. Journal of applied crystallography 40, 658–674, doi:10.1107/S0021889807021206 (2007).
Winn, M. D. et al. Overview of the CCP4 suite and current developments. Acta Crystallographica Section D: Biological Crystallography 67, 235–242, doi:10.1107/S0907444910045749 (2011).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66, 486–501, doi:10.1107/S0907444910007493 (2010).
Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallographica Section D: Biological Crystallography 60, 2126–2132, doi:10.1107/S0907444904019158 (2004).
Adams, P. D. et al. PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallographica Section D: Biological Crystallography 66, 213–221, doi:10.1107/S0907444909052925 (2010).
Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nature methods 9, 671–675, doi:10.1038/nmeth.2089 (2012).
Afonine, P. V., Grosse-Kunstleve, R. W. & Adams, P. D. A robust bulk-solvent correction and anisotropic scaling procedure. Acta Crystallographica Section D: Biological Crystallography 61, 850–855, doi:10.1107/S0907444905007894 (2005).
Tay, M. Y. et al. The C-terminal 18 Amino Acid Region of Dengue Virus NS5 Regulates its Subcellular Localization and Contains a Conserved Arginine Residue Essential for Infectious Virus Production. PLoS Pathog 12, e1005886, doi:10.1371/journal.ppat.1005886 (2016).
Kosugi, S. et al. Six classes of nuclear localization signals specific to different binding grooves of importin α. Journal of Biological Chemistry 284, 478–485, doi:10.1074/jbc.M807017200 (2009).
Wallace, A. C., Laskowski, R. A. & Thornton, J. M. LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein engineering 8, 127–134, doi:10.1093/protein/8.2.127 (1995).
de Beer, T. A., Berka, K., Thornton, J. M. & Laskowski, R. A. PDBsum additions. Nucleic acids research 42, D292–D296, doi:10.1093/nar/gkt940 (2014).
Laskowski, R. A. PDBsum: summaries and analyses of PDB structures. Nucleic acids research 29, 221–222, doi:10.1093/nar/29.1.221 (2001).
Laskowski, R. A. PDBsum new things. Nucleic acids research 37, D355–D359, doi:10.1093/nar/gkn860 (2009).
Laskowski, R. A., Chistyakov, V. V. & Thornton, J. M. PDBsum more: new summaries and analyses of the known 3D structures of proteins and nucleic acids. Nucleic Acids Research 33, D266–D268, doi:10.1093/nar/gki001 (2005).
Conti, E., Uy, M., Leighton, L., Blobel, G. & Kuriyan, J. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin α. Cell 94, 193–204, doi:10.1016/S0092-8674(00)81419-1 (1998).
Fontes, M. R. et al. Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-α. Biochemical Journal 375, 339–349, doi:10.1042/bj20030510 (2003).
Takeda, A. A., de Barros, A. C., Chang, C.-W., Kobe, B. & Fontes, M. R. Structural basis of importin-α-mediated nuclear transport for Ku70 and Ku80. Journal of molecular biology 412, 226–234, doi:10.1016/j.jmb.2011.07.038 (2011).
Mynott, A. V. et al. Crystal structure of importin‐α bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4. FEBS journal 278, 1662–1675, doi:10.1111/j.1742-4658.2011.08086.x (2011).
de Barros, A. C. et al. Structural and calorimetric studies demonstrate that Xeroderma pigmentosum type G (XPG) can be imported to the nucleus by a classical nuclear import pathway via a monopartite NLS sequence. Journal of molecular biology 428, 2120–2131, doi:10.1016/j.jmb.2016.01.019 (2016).
Acknowledgements
We would like to thank the staff and beamline scientists at the Australian Synchrotron for their valuable assistance during data collection.
Author information
Authors and Affiliations
Contributions
Experiments performed by Z.H., data collection by Z.H., S.T., J.K.F., and K.M.S., structure solved by J.K.F. and K.M.S., structure analysis by K.M.S., manuscript written by K.M.S. and J.K.F., all authors contributed to final manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare that they have no competing interests.
Additional information
Accession code: HIV-1 Tat accession code is AAF35362.1, importin-α is NP_034785.1, importin-β is NP_032405.3. The PDB code for the structure is 5SVZ.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Smith, K.M., Himiari, Z., Tsimbalyuk, S. et al. Structural Basis for Importin-α Binding of the Human Immunodeficiency Virus Tat. Sci Rep 7, 1650 (2017). https://doi.org/10.1038/s41598-017-01853-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-017-01853-7
- Springer Nature Limited
This article is cited by
-
MERS-CoV ORF4b employs an unusual binding mechanism to target IMPα and block innate immunity
Nature Communications (2022)
-
Genetic variation and function of the HIV-1 Tat protein
Medical Microbiology and Immunology (2019)