Abstract
Hypertonic saline (HS) attenuates cerebral edema, improves microcirculation perfusion and alleviates inflammation. However, whether the beneficial effect of HS on neurological function after cardiopulmonary resuscitation (CPR) in rat model of asphyxial cardiac arrest (CA) is mediated via attenuating apoptosis of neurons is not known. We studied the neuroprotective effect of HS in rats after CA and CPR, and explored the likely underlying mechanisms. Animals were randomly assigned to 4 equal groups (n = 15 each) according to the different infusions administered during resuscitation: control (C), normal saline (NS), hypertonic saline (HS), and hydroxyethyl starch (HES) groups. NDS at 12, 24, 48 and 72 h post-ROSC in the HS group were significantly higher than those in the NS and HES groups. Western blot analysis demonstrated a significant increase in Bcl-2 expression in HS, as compared to that in the NS and HES groups. However, Bax and Caspase-3 expressions in HS were significantly lower than that in the NS and HES groups. The apoptosis rate in HS was significantly lower than that in the NS and HES groups, suggesting HS treatment during resuscitation could effectively suppress neuronal cell apoptosis in hippocampal CA1 post-ROSC and improve neuronal function.
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Introduction
An estimated 54.4 million people sustain cardiac arrest (CA) in China each year1, of which only about 1% people survive2. According to a large multicenter registry-based observational study, up to 27% of all survivors of CPR sustain some form of neurological deficit3. The pathophysiologic mechanisms of brain injury following CA are complex and multifactorial, including the primary ischemic neuronal injury and secondary injury from consequent cerebral edema4. Therefore, it is very important to protect brain function during the advanced life support (ALS) treatment after CA and CPR. Many studies have indicated that neuronal apoptosis is one of underlying mechanisms of post-CA and post-CPR neuronal cell death5, 6. Ischemia during cardiac arrest and after restoration of spontaneous circulation (ROSC) will lead to neuronal necrosis, also reperfusion will aggravate this ischemic injury. Therefore, attenuation of neuronal apoptosis is a key strategy to maintain neurological function in these patients7.
Hypertonic saline is known to reduce intracranial pressure, promote osmotic diuresis and improve brain perfusion8. In addition, it can regulate immune function and has anti-inflammatory effect9. Both experimental (animal studies)10 and clinical research11 has shown hypertonic saline to increase the survival rates after CA and CPR and to improve neuronal function12. However, the underlying mechanism of this neuroprotective effect is not completely clear. Majority of research conducted till date has centered on the effect of hypertonic saline on inflammatory biomarkers and cerebral stroke area in focal brain ischemic or shock model9, 13, 14. However, the patho-mechanism of global cerebral ischemic insults resulting from asphyxia of CA is inherently different from focal cerebral impairment caused by brain artery occlusion15. Protecting brain cells by therapeutic methods such as mild hypothermia have been shown to suppress apoptosis5 and ameliorate neuronal damage16. Rüdiger et al. reported that hypertonic saline hydroxyethyl starch failed to ameliorate the newly generated hippocampal neurons in the survived CA and CPR rat models17. To date, it is not known whether hypertonic saline improves neurological function by attenuating apoptosis of brain injury after resuscitation in an asphyxial cardiac arrest rat model.
In the present study, we investigated the neuroprotective effect of 10% hypertonic saline on the rats after CA and CPR, and also attempted to explore the likely underlying mechanism.
Materials and Methods
Animal Preparation
After approval of the animal ethics committee and the animal care committee at the Wuhan general hospital of Chinese PLA (WH2015-13), 60 male Sprague-Dawley rats (250–300 g, Certificate No. 42009800001607) were provided by the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology. All rats were housed in a controlled environment (temperature 22 °C; free access to water and food and 12 hours’ light and 12 hours’ dark cycle. All rat treatment and care in details were in agreement with international institutional guidelines18.
Asphyxial cardiac arrest model
Experimental protocols on rats were performed based on Utstein-style guidelines for Uniform Reporting of Laboratory CPR Research19 and partly modified. In brief, each rat was anesthetized using 10% chloral hydrate (0.3 mL/100 g) intraperitoneal injection. A rectal probe was inserted and rectal temperature of rats was maintained at about 36 ± 1 °C during surgery with a biofeedback-heating mat under operating platform. The right femoral artery and vein were exposed via a skin incision along the right groin and blunt dissection of the subcutaneous tissue. Venous indwelling catheter (24G) was placed in the femoral artery and connected to a pressure transducer. Powerlab 16/30 (AD-Instruments, Australia) was used to monitor arterial blood pressure and electrocardiogram of normal lead II continuously. A 24G venous indwelling catheter was also inserted in the right femoral vein for continuous administration of fluid infusion via micro-perfusion pump at a rate of 2 mL/h. After orotracheal intubation using a teleflex of 16G venous indwelling catheter, median incision of neck was made on each rat, and blunt dissection of subcutaneous tissue performed to expose the trachea. In order to prevent air leakage, a firm ligature was placed on the trachea during mechanical ventilation of the rats (respiratory frequency 70 bpm, tidal volume 4 mL/100 g) by ventilator (ALC-V, Shanghai Alcott Biological Technology Co., Ltd.).
Under deep anesthesia, rats were prepared for the next experimental procedure. After 30 mins of venous infusion of different kinds of fluids (0.9% normal saline for C and NS groups, 10% hypertonic saline and hydroxyethyl starch 130/0.4 for HS and HES groups, respectively) and 10 minutes of mechanical ventilation, asphyxia was induced in rats by stopping the ventilator. At the same time, the tracheal tubes were also clamped tightly. CA was defined as the systolic blood pressure (SBP) < 25 mm Hg19. CPR was initiated after 5 mins of CA. Controlled ventilation was initiated at the same respiratory frequency of 70 bpm and tidal volume 4 mL/100 g, as administered earlier. External chest compressions were carried out with the instruction rhythm of metronome at the frequency of 200 compressions/min and the compression depth was 1/3 diameter of rat thorax anterior to posterior.
Adrenaline was injected (4 μg/100 g) through the right femoral venous indwelling catheter after 10 seconds of manual thoracic compressions. Restoration of spontaneous circulation (ROSC) was defined as the return of spontaneous sinus rhythm, with SBP > 60 mmHg which was maintained for at least 10 minutes. If ROSC was not achieved, animals were declared dead and excluded from the next experimental protocol. Controlled ventilation was continued for rats who survived the CA and CPR. Spontaneous respiration was carefully monitored every 5 minutes. If spontaneous respiration was good enough to be weaned from ventilator, the orotracheal tube was removed and the neck incision was closed. Venous indwelling catheters were withdrawn from the right femoral artery and vein, and venous infusion was stopped.
Experimental
Groups
Animals were divided into 4 groups (n = 15 for each group) using computer generated random numbers: C group (control group), NS group (normal saline group), HS group (hypertonic saline group) and HES group (hydroxyethyl starch group). Animals in the control group underwent anesthesia, venous infusion (2 mL/h) of normal saline and sham-operation, including placement of indwelling catheters in femoral artery and vein, orotracheal intubation and neck incision for blunt dissection of subcutaneous tissue which was similar to the experimental procedures, except CA and CPR procedures and adrenaline application. After surgery, all rats were subcutaneously injected with 1 mL of 5% Glucose Solution (GS) in the abdominal wall and placed in a 23 °C incubator with free access to food and water. Based on randomization, 30 mins before CA, rats in the NS, HS and HES groups received different venous infusions (2 mL/h) (normal saline (0.9%), hypertonic saline (10%) and hydroxyethyl starch (6%), respectively).
Basic measurements
Introperative monitoring of blood pressure, ECG, heart rate and rectal temperature was performed using Powerlab. The survival rates of CPR, as well as that at 6 h, 24 h, 48 h and 72 h after surgery were recorded.
Evaluation of neurologic deficit
Neurological examination and evaluation of neurological deficit was performed using neurological deficit scores (NDS) by an investigator who was blinded to the experimental design. NDS of the survived rats was assessed during the recovery period at 6, 12, 24, 48 and 72 hours after ROSC. NDS were determined on a scale of 0–80, on the basis of arousal level, cranial nerve reflexes, muscle tone, motor function, seizure and simple behavioral responses17, 20. Score 80 represented normal performances (no deficit); score 0 represented the brain death (most severe neurological deficit).
Hematoxylin and eosin (H&E) staining for hippocampus CA1 area
Euthanasia was performed 72 hrs after surgery under deep anesthesia. A whole brain was removed; hippocampus was isolated carefully and placed in 4% paraformaldehyde solution for further fixation for 24 hours. After conventional gradient ethanol dehydration, brain tissues was rendered transparent by dimethylbenzene and embedded in paraffin. Parallel coronal sections (4-μm thick) of paraffin-embedded brain tissue were prepared for immunohistochemical examination. Tissue sections were immersed in hematoxylin solution for 5–7 mins and washed in running water. Section differentiation was performed with acid alcohol (0.3%) and washed in running water again to remove excess dye. Sections were then stained with eosin solution (1%) for 2 mins and rinsed with running water for 30 seconds, repeatedly. Finally, brain tissue sections stained with H&E were sealed up with neutral balsam for visualization on biomicroscopy (NIKON, E100, Japan), after anhydrous alcohol dehydration, vitrification by dimethylbenzene and drying.
Western blot analysis for expression of apoptosis protein
Total protein was extracted from hippocampal tissue samples of euthanized rats 72 hrs after surgery, which were cryopreserved in liquid nitrogen (−196 °C). BCA Protein Assay Kit (Pierce, USA) was used to detect protein concentrations. Equal amounts of protein (25 μg) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to Polyvinylidene difluoride (PVDF) membrane (Millipore, USA), which was blocked with 5% nonfat milk for 2 hr. Then membranes were incubated with primary antibodies Bax, Bcl-2 and Caspase-3 (Proteintech, Wuhan, China. 1:1000) overnight at 4 °C. After washing with 1 × Tris-Buffered Saline and Tween 20 (TBST) 3 times, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Boster, Wuhan, China) at 37 °C for 2 hrs, then incubated shakily with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (1:50000, Abcam, Britain) for 2 hrs. Visualization was performed using an enhanced chemiluminescence detetion kit (Pierce, USA), and relative band intensities were quantified by use of BandScan (Glyko, ProZyme, USA).
Determination of apoptosis of hippocampal neurons in brain tissue by Tunel assay
Apoptosis of hippocampal neurons after resuscitation was detected by terminal deoxynucleotidyl transferase-mediated uridine 59-triphosphate-biotin nick end labeling (Tunel) assay. Coronal paraffin wax brain tissue sections were obtained as previously described21. In order to measure the apoptosis of hippocampal neurons, In Situ Cell Death Detection Kit (Roche, Switzerland) was used according to the manufacturer’s instructions. Apoptotic changes were detected via fluorescence microscopy (Olympus, Tokyo, Japan). Positive signal was indicated by green fluorescence staining region which represented apoptotic cells and blue fluorescence was stained by DAPI which represented cell nuclei. The images were obtained using a light microscope (Olympus, Japan). The apoptotic rate was defined as the ratio of green staining cells to blue staining cells. Three regions of interest were randomly chosen in each hippocampal CA1 section, the apoptotic rate calculated according to the formula mentioned above; mean of three measurements of apoptotic rate are presented.
Flow Cytometric Apoptosis Assays
72 hrs after CA and CPR, hippocampal tissue of rat was taken for preparation of flow cytometry apoptosis assays. Cells were collected after trypsinization. The apoptosis assays were measured by flow cytometers (FCM, BD Biosciences, USA) with Annexin V-FITC Apoptosis Detection Kit (KeyGEN BioTECH, Nanjing, China) according to the instruction of manufacturer.
Statistical analysis
A database was built using SPSS 20.0 for statistical analysis. Descriptive data are presented as mean ± SD or median (interquartile range) for continuous variables; categorical variables are presented as frequencies. Descriptive data were analyzed by repeated measures Analysis of Variance (ANOVA) or one-way ANOVA; Fisher exact test (Two-tailed) was used for categorical variables. P < 0.05 was considered as statistically significant.
Results
Resuscitation success and survival rate
There was no significant difference in resuscitation success rate between NS, HS and HES groups (P > 0.05, respectively). All 15 rats in the control group survived 72 hrs after surgery. Survival rates at 24, 48 and 72 hours after surgery in the NS group were significantly lower than those in the control group (P < 0.05). Survival rates at 48 and 72 hrs after surgery in the HES group were significantly lower than those in the control group (both P < 0.05). In contrast, no significant difference in survival rates at 6, 24, 48 and 72 hours was observed between the HS and control groups (P > 0.05, respectively; Table 1).
Rat NDS scores post-ROSC in 4 groups
NDS at 6, 12, 24, 48, and 72 hours post-ROSC in the NS, HS and HES groups were significantly lower than that in the C group (P < 0.001, respectively); NDS at 12, 24 and 48 hours in the HS group were significantly higher than those in the NS and HES groups (12 h: P1 = 0.001, P2 = 0.007; 24 h: P < 0.001, P2 = 0.012; 48 h: P1 = 0.002; P2 = 0.046); NDS at 72 hours in the HS group were significantly higher than those in the NS group (P = 0.004. Fig. 1).
Changes in mean arterial pressure (MAP) at different time points
No significant between-group difference with respect to MAP was observed at baseline, before asphyxia, and at 20 and 25 minutes post-ROSC between the C, NS, HS and HES groups (P > 0.05); however, the immediate MAP at 0 min and 15 min post-ROSC in the NS, HS and HES groups, were significantly lower than that in the control group (P < 0.001, respectively). MAP at 5 min post-ROSC in the NS, HS and HES groups were significantly higher than that in the control group (P < 0.001, respectively). MAP at 10 min post-ROSC in the HS and HES groups were higher than that in the NS group (P < 0.05, respectively). Similarly, MAP at 30 min post-ROSC in the NS, HS and HES groups was significantly lower than that in the control group (P < 0.05, respectively. Fig. 2).
Duration of different phases and volume of fluid infused in each phase of the establishment of rat model of CPR
There were no significant differences with respect to duration of the key phases of CPR, including time for establishment of CA model; duration of asphyxia and CPR; duration of mechanical ventilation and total time between the NS, HS and HES groups (P > 0.05, respectively). Further, there was no significant different between these 3 groups with respect to the volume of fluid injected by microinfusion pump during the experiment (P > 0.05 for all; Table 2).
P > 0.05 for all. Time duration for establishment of CA model, from beginning of anesthesia to completion of blunt dissection of trachea in the neck median incision; Duration of asphyxia, from clamping the tracheal tube to cardiac arrest; Duration of CPR, from performing external chest compressions to restoration of spontaneous circulation (ROSC); Total time, from beginning of anesthesia to removal the orotracheal tube and closing the neck incision; Infusion volume, the liquid volume injected by microinfusion pump during the whole experimental procedure.
Pathological changes of Hippocampal CA1 by H&E staining
Neuronal cells in Hippocampal CA1 in control group were well-formed, round or oval in shape and -arranged well. Nuclear staining was weak and the chromatin was relatively dispersed. (Fig. 3a). However, the neuronal cells in the NS and HES groups were arranged in significantly disordered manner. Nuclear staining was purple red with chromatin condensation. Obvious cell swelling, degeneration and necrosis of cells were observed (Fig. 3b and d). Contrarily, cells in the HS group were arranged with a little disorder. Nuclear staining was purple red with chromatin condensation with a moderate number of degenerative cells. Mild to moderate degree of cell swelling was observed (Fig. 3c).
Effect on apoptosis protein expression of Bcl-2, Bax and Caspase-3
Different infusion fluids used in the experiment appeared to have different effects on the expression of apoptosis protein, Bcl-2, Bax and Caspase-3. Western blot analysis demonstrated a significant decrease in Bcl-2 expression in the NS and HES groups (P < 0.001 vs. control for both groups). In contrast, a significant increase in Bcl-2 expression was observed in HS group as compared to that in the NS and HES groups (both P < 0.01), while there was no difference with that in the control group (P = 0.432). Bax expression levels in NS, HS and HES were significant higher than that in the control group (P < 0.001 for all three groups). Bax expression in the HS group was significantly lower than that in the NS and HES groups (P < 0.001 for both). Caspase-3 expression levels in NS, HS and HES were significantly higher than that in the control group (P < 0.001, for all 3 groups). However, Caspase-3 expression in HS was significant lower than that in the HS and HES groups, (P < 0.001 for both respectively, Fig. 4A and B).
Evaluation of CA-induced neuronal cell loss
TUNEL assay of Hippocampal CA1 neuronal cells
At 72 hrs after ROSC, apoptotic rate of neuronal cells in hippocampal CA1 in the NS, HS and HES groups were significantly higher than that in the control (P < 0.001 for all). However, the apoptotic rate in HS was significantly lower than that in the NS and HES groups (P < 0.001 for both; Fig. 5A and B).
Hippocampal CA1 neuronal cell apoptotic rate by Flow cytometry assay
The apoptosis rates of neuronal cells in hippocampal CA1, as assessed on flow cytometry assay, in the NS, HS and HES groups were significantly higher than that in the control group (P < 0.001 for all). However, the apoptotic rate in HS group was lower than that in the NS and HES groups (P < 0.001 and P = 0.047, respectively; Fig. 6A and B).
Discussion
In the present study, use of hypertonic saline infusion during resuscitation significantly inhibited the expression of apoptosis proteins Bax, and Caspase-3 and enhanced the expression of anti- apoptosis protein Bcl-2. Further, hypertonic saline infusion significantly decreased the apoptotic rate of CA-induced hippocampal neuronal cells 72 hrs post-ROSC. Neurological function, as assessed by NDS, at 12, 24, 48 and 72 hrs after ROSC. Our results are consistent with those reported elsewhere10, 11.
Although some reports have shown similar neuroprotective effects of HS treatment during resuscitation, for example one randomised study of hypertonic saline infusion during resuscitation from out-of-hospital cardiac arrest in Germany12, very few studies have investigated the underlying mechanism for this phenomenon. In our study, for the first time, we have explored the mechanism of the neuroprotective effect and showed that hypertonic saline infusion suppressed apoptosis of hippocampal cells in a rat model of cardiopulmonary resuscitation.
The pathophysiologic mechanisms of ischemic brain damage caused by cardiac arrest and cardiopulmonary-cerebral resuscitation are complicated. These mechanisms consist of the primary insult of cardiac arrest (complete temporary global brain ischemia) and secondary derangements during and after reperfusion-reoxygenation22.
Sudden cardiac arrest can induce global brain ischemia and subsequently result in irreversible cerebral damage. Even after return of spontaneous circulation, reperfusion injury, cerebral edema and inflammation could exist for long time and lead to massive apoptosis of neuronal cells17, 23. It is well known that neuronal cell apoptosis is one of the main pathological change induced by CA post-ROSC, which is also the main form of brain cell death. Therefore, neuronal cell apoptosis may cause severe damage to neuronal function5. This impacts the post-resuscitation restoration of neuronal function. In our study, the apoptotic rate of CA-induced hippocampal neurons 72 hrs post-ROSC was up to 43%.
Hippocampal CA1 has been shown to be most vulnerable to ischemia in brain24 and especially, the neuronal cell apoptosis in Hippocampal CA1 after resuscitation is usually observed by H&E staining similar to our study. Suppression of apoptosis of hippocampal cells after cardiopulmonary resuscitation is an important strategy to ameliorate secondary neuronal dysfunction7. Results of Tunel assay and flow cytometry indicated anti-apoptotic effect of HS on Hippocampal CA1 neuronal cells after CA and CPR. Based on our study and previous works, the underlying mechanism maybe as follows:
-
(1)
HS treatment reduced cerebral edema and the damage of blood brain barrier.
CA-induced brain ischemia could result in global cerebral edema, which may directly lead to high intracranial pressure during resuscitation. It is well known that lower intracranial pressure is associated with good prognosis post-ROSC25. Decompressive craniectomy was shown to decrease the apoptotic rate of neuronal cells in rat brain cells after CA and CPR26, also decreased the length of stay in the ICU in clinical27. Hypertonic saline, as a new dehydrating agent, has been applied in more and more studies28. A large body of evidence shows that hypertonic saline can significantly reduce intracranial pressure and enhance cerebral perfusion pressure29, whether in the early phase of post-ischemic global cerebral cell edema or in the later phase of vasogenic brain edema induced by impaired blood brain barrier30. HS was shown to be superior to mannitol, a traditional dehydrating agent, in attenuating cerebral edema, and working longer31.
In addition, disruption of blood brain barrier was shown to play a major role in CA- induced brain injury by causing cerebral vascular insult32. The close association of cerebral edema and brain neuronal cell apoptosis with increased permeability of blood brain barrier is well-documented33. Importantly, previous hypertonic saline infusion was shown to ameliorate the disruption of blood brain barrier, protect integrity of cerebral vasculature and attenuate vasogenic brain edema34, which indicates HS to be a good agent for reducing intracranial pressure35, improving cerebral perfusion and for lowering neuronal apoptosis. Additionally, hypertonic saline was shown to inhibit the expression of vascular endothelial growth factor (VEGF), which is closely related to cerebral vascular integrity and is known to regulate the permeability of blood brain barrier. Besides, hypertonic saline has the protective effect on tight junctions within endothelial cells of brain vasculature36.
-
(2)
HS treatment improved cerebral blood perfusion.
Hypotension after CA and CPR is known to exacerbate brain injury37. Maintenance of an optimal level of MAP (80–100 mmHg) is a key strategy for resuscitation. HS infusion induces immediate increase in blood osmotic pressure and effective blood volume and improves perfusion. Studies have showed that HS infusion maintains higher MAP by inducing sustained sympathetic nerve excitation, increases heart rate and augments strengthens myocardial contractility10. However, in our study, no significant difference was observed between the HS and HES groups with respect to post-resuscitation MAP. In contrast, a significant difference with respect to neuronal cell apoptotic rate was observed between the 2 groups; with the HES group showing a higher apoptotic rate (36.83%). Therefore, the mechanism of HS treatment which inhibited the neuronal cell apoptotic rate post-ROSC did not appear to be related to the MAP.
Also, CA-induced global cerebral ischemia might have led to microvascular endothelial swelling and proliferation of the surrounding glial cells with swollen glial foot, which further induces compressive deformity and obstruction of the microvasculature. Many studies have shown that HS infusion regulates cerebral and cardiac microcirculation by attenuating cerebral cell edema, ameliorating microvascular endothelial swelling and reducing the microvascular resistance9, 38. We could speculate that the HS effect of increasing microcirculation may be one of the mechanisms of the anti-apoptotic effect of HS treatment on Hippocampal CA1 after CA and CPR.
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(3)
HS treatment affected the expression of apoptosis protein.
More and more attention is being paid on the relationship of apoptosis protein and cerebral injury5. Previous studies have shown significant changes in expressions of Bcl-2, Bax and caspase-3 proteins in cerebral neuron injury, while they exerted a significant effect on neuronal cell apoptosis. High expression of Bcl-2 had an anti-apoptotic effect. On the contrary, up-regulation of Bax increased apoptosis39. Some neuroprotective drugs can exert anti-apoptotic effects via regulating Bcl-2 and Bax expression during ischemia–reperfusion pathology process40. Bcl-2 and Bax form heterodimers, which play an important role in apoptosis, such as transduction of apoptotic signals. Caspase-3 is a key enzyme for activation of caspases of apoptosis pathways41. To some extent, changes in caspase-3 expression may reflect the degree of cell apoptosis42.
In present study, Bcl-2 expression level in HS group was significantly higher than that in the NS and HES groups. Bax and Caspase-3 in the HS group were significantly lower than that in the NS and HES groups. Therefore, the anti-apoptotic effect of hypertonic saline infusion appears to have been mediated via up-regulation of anti-apoptotic protein and down-regulation of the pro-apoptotic proteins.
-
(4)
HS treatment ameliorated inflammation during resuscitation.
CA-induced cerebral ischemia triggers the host defense response to injury. Inflammation is one of the major pathological pathways involved in defense response to injury. Inflammatory cascades could be triggered within minutes of the onset of asphyxia43 and release inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and monocyte chemotactic protein-1 (MCP-1)13, 43. Increasing evidences shows that HS can alleviate cerebral inflammation by inhibiting the release of microglia-derived inflammatory cytokines13. Besides, the protective effect of HS on the integrity of the blood brain barrier may also prevent inflammatory cytokines from entering the neurovasculature. It is possible that the attenuation of inflammation by HS treatment was associated with its’ anti-apoptotic effect. We did not evaluate the inflammation during resuscitation.
Conclusion
In this study, HS treatment during resuscitation inhibited neuronal cell apoptosis in hippocampal CA1 at 72 hrs post-ROSC, and improved neuronal function as assessed by NDS. The likely mechanism of action is attenuation of cerebral edema; improvement in microcirculation perfusion; regulation of the balance of pro-apoptotic and anti-apoptotic proteins and alleviation of inflammation.
Limitations
In our study, we studied the model of pretreatment with different fluids before cardiac arrest. But in clinical practice, cardiac arrest is more often due to cardiac etiology and always occurs unexpectedly44. In next study, extensive research will be performed using different cardiac arrest models from cardiac etiology and asphyxia using different fluid infusion. Also, we did not assess expressions of inflammatory cytokines in this study and the corresponding mechanism of the relationship of inflammation and apoptosis during resuscitation. Further research would be needed to evaluate these aspects.
References
Yan, H. & Li, B. The clinical analysis of the effect of simultaneous treatment of mild hypothermia therapy and cardio-pulmonary resuscitation pre-hospital. Beijing Medical Journal 37, 1089–1091 (2015).
Hua, W. et al. Incidence of sudden cardiac death in China: analysis of 4 regional population. J. Am.Coll.Cardiol. 54, 110–1118 (2009).
Nadkarni, V. M. et al. First documented rhythm and clinical outcome from in-hospital cardiac arrest among children and adults. JAMA. 295, 50–57 (2006).
Morimoto, Y., Kemmotsu, O., Kitami, K., Matsubara, I. & Tedo, I. Acute brain swelling after out-of-hospital cardiac arrest: pathogenesis and outcome. Crit Care Med. 21, 104–110 (1993).
Li, H. & Wang, D. Mild hypothermia improves ischemic brain function via attenuating neuronal apoptosis. Brain. Res. 1368, 59–64 (2011).
Chalkias, A. & Xanthos, T. Post-cardiac arrest brain injury: pathophysiology and treatment. J Neurol Sci. 315, 1–8 (2012).
Namura, S., Ooboshi, H., Liu, J. & Yenari, M. A. Neuroprotection after cerebral ischemia. AnnNY. AcadSci. 1278, 25–32 (2013).
Wijdicks, E. F. et al. Recommendations for the management of cerebral and cerebellar infarction with swelling: a statement for healthcare professionals from the American Heart Association/American Stroke Association. Stroke 45, 1222–1238 (2014).
Cao, C. et al. Hypertonic saline reduces lipopolysaccharide-induced mouse brain edema through inhibiting aquaporin 4 expression. Critical Care. 16, 1–10 (2012).
Li, W., Xu, J., Tan, D. Y. & Yu, X. The efficacy of hypertonic saline treatment in cardiopulmonary resuscitation in animal model with cardiac arrest: a Meta-analysis. Chin. Crit. Care. Med. 27, 197–202 (2015).
Hahn, C. et al. Hypertonic saline infusion during resuscitation from out-of-hospital cardiac arrest: a matched-pair study from the German Resuscitation Registry. Resuscitation. 85, 628–636 (2014).
Breil, M. et al. Randomised study of hypertonic saline infusion during resuscitation from out-of-hospital cardiac arrest. Resuscitation 83, 347–352 (2012).
Huang, L. Q. et al. Hypertonic saline alleviates cerebral edema by inhibiting microglia-derived TNF-alpha and IL-1 beta-induced NA-K-CL Cotransporter up-regulation. J. Neuroinflammation. 11, 1–20 (2013).
Motaharinia, J., Etezadi, F., Moghaddas, A. & Mojtahedzadeh, M. Immunomodulatory effect of hypertonic saline in hemorrhage shock. Daru Journal of Pharmaceutical Sciences 23, 1–8 (2015).
Gao, C. J. et al. Effects of intracerebroventricular application of the delta opioid receptor agonist [DAla2, D-Leu5] enkephalin on neurological recovery following asphyxial cardiac arrest in rats. Neuroscience 168, 531–542 (2010).
Järnum, H. et al. Diffusion and perfusion MRI of the brain in comatose patients treated with mild hypothermia after cardiac arrest: a prospective observational study. Resuscitation. 80, 425–30 (2009).
Noppens, R. R. et al. Effects of a single-dose hypertonic saline hydroxyethyl starch on cerebral blood flow, long-term outcome, neurogenesis, and neuronal survival after cardiac arrest and cardiopulmonary resuscitation in rats. Crit. Care. Med. 40, 2149–2156 (2012).
Council, N. Guide for the Care and Use of Laboratory Animals (8th edition). Guide for the Care & use of Labotatory Animals 327, 41–48 (1974).
Idris, A. H. et al. Utstein-style guidelines for uniform reporting of laboratory CPR research. Circulation. 94, 2324–2336 (1996).
Jia, X. et al. Early electrophysiologic markers predict functional outcome associated with temperature manipulation after cardiac arrest in rats. Crit. Care. Med. 36, 1909–1916 (2008).
Nga, V. D. et al. Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation. Tissue Eng Part A 21, 647–653 (2015).
Vaagenes, P. et al. Cerebral resuscitation from cardiac arrest: pathophysiologic mechanisms. Crit. Care. Med. 24, S57–68 (1996).
Yang, L. et al. Delta opioid receptor agonist BW373U96 attenuates post_resuscitation brain injury in a rat model of asphyxial cardiac arrest. Resuscitation 85, 299–305 (2014).
Sugawara, T. et al. Effect of hypotension severity on hippocampal CA1 neurons in a rat global ischemia model. Brain. Res. 877, 281–287 (2000).
Moore, J. C., Bartos, J. A., Matsuura, T. R. & Yannopoulos, D. The future is now: neuroprotection during cardiopulmonary resuscitation. Curr Opin Crit Care. 24, doi:10. (2017).
Feng, K. et al. Effect of decompressive craniectomy on cerebral resuscitation of cardiac arrest rats. Journal of C1inical Medicine in Practice 19, 5–8 (2015).
Cooper, D. J. et al. Decompressive craniectomy in diffuse traumatic brain injury. N. Engl. J. Med. 364, 1493–502 (2011).
Kamel, H., Ngvi, B. B., Nakagawa, K. & Ko, N. U. Hypertonic saline versus mannitol for the treatment 0f elevated intracranial pressure: a meta-analysis of randomized clinical trials. critical Care Medicine 39, 554–559 (2011).
Surani, S., Lockwood, G., Macias, M. Y., Guntupalli, B. & Varon, J. Hypertonic saline in elevated intracranial pressure past, present, and future. Journal 0f Intensive Care Medicine 41, 149–151 (2013).
Krep, H. et al. Effects of hypertonic versus isotonic infusion therapy on regional cerebral blood flow after experimental cardiac arrest cardiopulmonary resuscitation in pigs. Resuscitation. 63, 73–83 (2004).
Liu, S. et al. Superior effect of hypertonic saline over Mannitol to attenuate cerebral edema in a rabbit bacterial Meningitis model. Crit. Care. Med. 39, 467–1473 (2011).
Li, J. et al. Mild hypothermia alleviates brain oedema and blood-brain barrier disruption by attenuating tight junction and adherens junction breakdown in a swine model of cardiopulmonary resuscitation. PLoS One. 12, e0174596 (2017).
Chen, M. et al. Baicalein Attenuates Neurological Deficits and Preserves Blood-Brain Barrier Integrity in a Rat Model of Intracerebral Hemorrhage. Neurochem Res. 41, 3095–3102 (2016).
Nakayama, S., Migliati, E., Amiry-Moghaddam, M., Ottersen, O. P. & Bhardwaj, A. Osmotherapy With Hypertonic Saline Attenuates Global Cerebral Edema Following Experimental Cardiac Arrest via Perivascular Pool of Aquaporin-4. Crit Care Med. 44, e702–10 (2016).
Mangat, H. S. et al. Hypertonic saline reduces cumulative and daily intracranial pressure burdens after severe traumatic brain injury. J. Neurosurg. 122, 202–210 (2015).
Hu, X. L. & Qi, D. Y. Effect of hypertonic saline on permeability of blood-brain barrier in a rat model of intracerebral hemorrhage. Chin. J. Anesthesiol. 35, 1014–1016 (2015).
Callaway, C. W. et al. Part 8: Post-Cardiac Arrest Care: 2015 American Heart Association Guidelines Update for Cardiopulmonary Resuscitation and Emergency Cardiovascular Care. Circulation. 132, S465–82 (2015).
Breil, M. et al. Hypertonic saline improves myocardial blood flow during CPR, but is not enhanced further by the addition of hydroxy ethyl starch. Resuscitation. 56, 307–317 (2003).
Liu, G., Wang, T., Wang, T., Song, J. & Zhou, Z. Effects of apoptosis-related proteins caspase-3, Bax and Bcl-2 on cerebral ischemia rats. Biomed. Rep. 1, 861–867 (2013).
Xi, H. J. et al. Propofol improved neurobehavioral outcome of cerebral ischemia- reperfusion rats by regulating Bcl-2 and Bax expression. Brain. Res. 1410, 24–32 (2011).
Li, J., Han, B., Ma, X. & Qi, S. The effects of propofol on hippocampal caspase-3 and Bcl-2 expression following forebrain ischemia reperfusion in rats. Brain. Res. 1356, 11–23 (2010).
Niizuma, K. et al. Mitochondrial and apoptotic neuronal death signaling pathways in cerebral ischemia. Biochim. Biophys. Acta. 1802, 92–99 (2010).
Siniscalchi, A. et al. Cerebral stroke Injury: the role of cytokines and brain inflammation. J. Basic. Clin. Physiol. Pharmacol. 25, 131–137 (2014).
Hossmann, K. A. Cerebral ischemia: models, methods and outcomes. Neuropharmacology 55, 257–70 (2008).
Acknowledgements
This work was part of the Program funded under the national natural science foundation of China, grant number 81471831. We thank the Laboratory of cardiac surgery of Tongji Medical College of HuaZhong University of Science and Thchnology for the help of Providing experimental equipment and technical guidance.
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ShuiBo Zhu and Yong Liu conceived and designed the study. Xiang Zhou and Yang Huang performed the experiments. Xiang Zhou, Jian Zhu and Rong Ping Wang wrote the paper. All authors read and approved the manuscript.
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Zhou, X., Liu, Y., Huang, Y. et al. Hypertonic saline infusion suppresses apoptosis of hippocampal cells in a rat model of cardiopulmonary resuscitation. Sci Rep 7, 5783 (2017). https://doi.org/10.1038/s41598-017-05919-4
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DOI: https://doi.org/10.1038/s41598-017-05919-4
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