Abstract
Despite availability of sequence site-specific information resulting from years of sequencing and sequence feature curation, there have been few efforts to integrate and annotate this information. In this study, we update the number of human N-linked glycosylation sequons (NLGs), and we investigate cancer-relatedness of glycosylation-impacting somatic nonsynonymous single-nucleotide variation (nsSNV) by mapping human NLGs to cancer variation data and reporting the expected loss or gain of glycosylation sequon. We find 75.8% of all human proteins have at least one NLG for a total of 59,341 unique NLGs (includes predicted and experimentally validated). Only 27.4% of all NLGs are experimentally validated sites on 4,412 glycoproteins. With respect to cancer, 8,895 somatic-only nsSNVs abolish NLGs in 5,204 proteins and 12,939 somatic-only nsSNVs create NLGs in 7,356 proteins in cancer samples. nsSNVs causing loss of 24 NLGs on 23 glycoproteins and nsSNVs creating 41 NLGs on 40 glycoproteins are identified in three or more cancers. Of all identified cancer somatic variants causing potential loss or gain of glycosylation, only 36 have previously known disease associations. Although this work is computational, it builds on existing genomics and glycobiology research to promote identification and rank potential cancer nsSNV biomarkers for experimental validation.
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Introduction
Protein glycosylation is the enzymatic process by which a carbohydrate is covalently attached to a target protein, and is a form of co-translational or post-translational modification (PTM)1. It is the most complex protein modification due to the diversity of glycans and possible branching configurations2, and it impacts protein characteristics, including folding, stability, cell motility, and cell-cell adhesion1,3,4, that could be implicated in disease. Glycosylation is an enzyme-directed, site-specific process: a complex network of enzymatic pathways is responsible for the broad variety of glycan structures, and the site-specific structural diversity of glycosylated proteins determines functionality1,4.
Glycoproteins belong primarily to two groups: N-linked and O-linked1,5. N-glycans are covalently linked to the carboxamide (-CONH2) group of an asparagine residue in the first position of the specific N-linked glycosylation sequon (NLG), NXS/T (X! = P), where N is asparagine, S/T denotes either serine or threonine, and X is any amino acid except proline6. Occasionally, glycosylation may occur when a cysteine (C) is in the third position of the sequon instead of an S or T7,8. Not all NLGs are glycosylated due to the limited accessibility and intricate regulation of glycosylation-related enzymes9, and other sequence motifs for the attachment of N-glycans have been described but are utilized with much lower frequency10.
While the clinical relevance of glycosylation was reported several years ago11, the last two decades have seen an increase of human glycosylation profiling studies due to technological advances including high-throughput sequencing (HTS)12,13,14,15 and glycomics approaches16,17,18,19. A number of sequence and feature annotation databases now exist20,21,22,23,24,25, including the Universal Protein Knowledgebase (UniProtKB)26, which provides a consistent and richly annotated repository of protein function, interaction, disease relatedness, and other characteristics26. The Swiss-Prot section of UniProtKB provides additional information concerning biological pathways, PTMs, and disease variants27, collected by multiple approaches28,29 and subject to manual review by experts.
Using data available through UniProtKB/Swiss-Prot, Apweiler et al. published a comprehensive study about the distribution of NLGs across all organisms in 199930, reporting that almost two thirds of proteins contained the NX(S/T) (X! = P) sequon, and predicting that most sequon-containing proteins would eventually be confirmed as true glycoproteins. According to release statistics, the amount of protein entries and associated annotations in UniProtKB/Swiss-Prot has since drastically changed: there are currently more than 550,000 UniProtKB/Swiss-Prot entries representing more than 10,000 species, with humans being the most represented species with respect to number of protein entries. Despite this growth, there has not yet been a formal update of the NLG distribution in humans. Thus, our first research objective for this study was to use current data to provide new statistics regarding the distribution and occupancy rate of NLGs among human proteins. Note that although glycosylation has been observed at NXC sites, we limit the scope of this study to the traditional NXS/T sequon at this time due to the relative rarity of verified glycosylation at atypical sequons.
While loss and gain of glycosylation can be a factor during evolution and is not necessarily damaging31, specific changes in human glycosylation have been associated with pathology or physiology of processes like embryonic development or carcinogenesis and can be explored efficiently as biomarkers1,32,33,34. Notable changes observed in glycan profiles are a consequence of environmental influences and physiologic responses and therefore could have a significant diagnostic potential3. For example, the presence of certain glycoconjugate species has been shown to interact with and potentially regulate cancer cell processes, tumor malignancy, and tumor microenvironment35,36,37,38.
Specifically, variations impacting NLGs could directly cause disease by altering glycosylation; this hypothesis is consistent with the observations that the majority of human inherited disease missense mutations affect protein stability39, and that N-linked glycosylation decreases protein dynamics and likely increases stability40. In fact, there are a number of cases demonstrating the role of loss and gain of glycosylation in disease. For example, the loss of an NLG in the prion protein (PRNP) due to a T183A substitution leads to spongiform encephalopathy41,42, and sequon loss by a T315A in Vitamin-K dependent protein C is associated with venous thromboembolism43. Conversely, a gain of glycosylation results from the well-characterized D356N germline variation in the sex hormone-binding globulin protein (SHBG), which generates an additional N-linked site that has been observed with an attached carbohydrate chain. This variation and gain of SHBG glycosylation corresponds to a decreased SHBG clearance rate, an increased half-life, and increased circulating levels of the protein, both in humans and animals44,45,46. Additionally, sequon gain via A349S in Integrin alpha-3 and its subsequent hyperglycosylation results in multiorgan failure47. Although variations involving gain of glycosylation sequons (GOGs) have been considered rare, up to 1.4% of known disease-causing missense mutations involving human genetic diseases can lead to GOGs; in some cases, attachment of novel glycans at these sites is sufficient to account for the detrimental effect of the corresponding variation48,49.
The above-referenced technological advances have paved the way for studies concerning the functional importance of N-linked glycosylation in disease, including cancer35,50. There is now evidence that β1,6GlcNAc-branching of N-glycans directly contributes to cancer progression, and both GlcNAc-branched N-glycans and terminal Lewis antigen sequences have been observed to increase in some cancers and are related with poor prognosis51. In breast cancer, the frequency of the aforementioned SHBG D356N variant was significantly higher in estrogen-dependent cases and suggested a close association between the additionally glycosylated variant and the estrogen-dependence of breast cancer52. Interestingly, this variant is distributed with different frequencies among different ethnic groups53. Thus, our second major research objective of this study was to provide a pan-cancer view of the impact of nucleotide variation on the human N-linked glycosylation profile.
Restated, we conducted a comprehensive analysis of nsSNVs that may lead to either loss or gain of NLGs and compared functional impacts of germline SNPs and somatic nsSNVs, especially with respect to cancer involvement. While independent genomics databases provide variation data from cancer samples and literature mining tools can extract disease and variation information, this work is the first comprehensive effort toward the integration of N-linked glycosylation data with nsSNVs from multiple cancer genomics databases with value-added information and high-value curations from additional resources (such as dbSNP, ClinVar, UniProtKB/Swiss-Prot, and publications). Integrated data were then mapped to Disease Ontology (DO)54 terms to facilitate a comparative functional impact analysis55 of nucleotide variation. Synthesis of this information allowed for ranking proteins for which variation may affect N-glycosylation in cancer. We suggest high-confidence proteins and/or variants represent ideal candidates for downstream validation with respect to both glycosylation status and cancer involvement. Note that while we do prioritize these variants based on relevance of functional annotations and presence in multiple cancers for the purposes of targeted future study, we do not distinguish between the roles of glycosylation-impacting variants across multiple cancers or within a distinct cancer type.
Results
Comprehensive collection of real and predicted NLGs
Overview of human NLGs
Table 1 summarizes the findings from each of the three methods of NLG identification: high-confidence NLGs (those reported in databases with validated evidence or manual assertion), predicted NLGs by NetNGlyc (http://www.cbs.dtu.dk/services/NetNGlyc/), and string search of all NX(S/T) (X! = P) sequons. Proteins predicted to contain signal peptides or annotated with the UniProt keyword(s) “Secreted” or “Membrane” were used for analysis of disease-relatedness to focus on those entries with the greatest potential of being biologically viable biomarkers. Of the 59,341 identified non-redundant NLGs from 15,318 proteins (See Supplemental Table S1), 7,017 of these proteins either have signal peptides or are annotated with one or both keyword(s) “Secreted” or “Membrane,” prioritizing this subset for consideration as biologically viable markers. Note that of the 15,318 total proteins identified to contain NLGs and the subset of 7,017 likely viable N-linked glycosylation sites identified by functional annotations, 15,314 and 7,014, respectively, were retrievable by the string search method alone. Additional information about these NLG-containing sequences, including sequence length, existence and positions of signal peptides, “Cellular component” keywords, and more, can be viewed in Supplemental Table S1.
Frequency of NLGs and experimentally validated sites in human proteins
Within the UniProtKB/Swiss-Prot dataset, there are 15,318 of 20,199 human proteins, or 75.8%, of all human proteins with at least one NLG. Of the 59,341 NLGs on these human proteins, for an average of 3.9 per protein, 16,253 of 21,956, or 74.03% of total NLGs from 4,412 glycoproteins are well-characterized N-linked glycosylation sites. In this context, the term glycoprotein is applied based on the observation of at least one occupied NLG. Among the high-confidence sites, 2,511 NLGs are experimentally verified with evidence from publications and 13,500 are manually asserted by UniProtKB/Swiss-Prot curators. 232 additional NLGs were retrieved from other databases and are included in the table of potential NLGs (see Materials and Methods section). Figure 1 shows the relative contribution of sources to the identification of NLGs. Furthermore, 61 NLGs belonging to these high-confidence N-linked glycosylation sites are atypical in that the corresponding UniProtKB/Swiss-Prot FT line annotations do not follow the consensus NX(S/T) (X! = P) configuration. Therefore, although the current rate of experimentally validated human NLGs is 27.39%, the rate of experimentally validated NLGs in the subset of known glycoproteins is 74.03%, aligning with Apweiler’s previous hypothesis that three quarters of glycoproteins should be N-linked.
Distribution and spacing of NLGs and experimentally validated N-linked glycosylation sites
The distribution of NLGs per protein is shown in Fig. 2 (for more information, see Supplemental Table S2). Most proteins have a small number of NLGs, but some proteins have large numbers of NLGs. The most extreme example of this case is Mucin-16 (MUC16, UniProtKB accession: Q8WXI7), which has 265 NLGs. As the largest cell-associated mucin, the length of MUC16 at 22,152 residues56 increases the likelihood for multiple NLGs in the sequence. Mucins are a major component of mucus with a normal protective physiological role, but it has been shown that glycan attachment to MUC16 is altered in response to oxidative stress in pancreatic cancer57 and is a major carrier of altered sialylation characteristic of malignant conditions in serous ovarian tumors58. When normalized by length, MUC16 still shows a greater than average number of NLGs per unit length (Table 2), but Sialomucin core protein 24 (CD164, Q04900) has the greatest density of NLGs at approximately one per every 22 residues, compared to the average of approximately one per every 118 residues. CD164 belongs to a class of heavily glycosylated proteins, the sialomucins, involved in regulation of cellular adhesion, proliferation, differentiation, and migration of hematopoietic stem cells59. Although its expression, not glycosylation, has been implicated in various cancers60,61, absence of proper terminal N-glycan attachment on CD164 protein has been observed to prevent functional interactions, disrupting post-endocytic sorting of the protein59. Table 2 demonstrates similar trends in the spacing of both total NLGs and verified N-linked glycosylation sites when averaged for all proteins.
Summary of collected variation data
A total of 1,272,878 somatic nsSNVs collected across cancer genomics databases are associated with 59 cancer types which are mapped to the Cancer Disease Ontology (CDO)62. There are also 3,680,786 germline SNPs from dbSNP.
Identification of LOG and GOG resulting from variation
Mapping of nsSNVs to NLGs led to identification of variations that may lead to loss of glycosylation (LOG) and gain of glycosylation (GOG). In some cases, different variations can lead to abolition or creation of the same NLG, and proteins may contain multiple NLGs. Thus, we expect the number of variations to be greater than or equal to the number of affected sequons, which is expected to be greater than or equal to the number of affected proteins. Our observations follow this trend, as can be seen in Table 3 (additional information about LOG and GOG variations and corresponding sequons can be found in Supplemental Tables S3–S8).
In this study, we report 16,253 high-confidence (experimentally validated or manually asserted) sites with 2,898 and 7,508 sequons abolished by somatic and germline variations, respectively. Although experimental validation of GOG is outside the scope of this study, we report 15,504 and 38,711 potentially induced sequons by somatic and germline variations, respectively. Furthermore, in this update we report that 3,930 of 4,412 high-confidence glycoproteins lose or gain NLGs due to germline or cancer somatic nsSNV compared to 1,091 human proteins previously reported to undergo LOG or GOG due to polymorphisms63. For all datasets, the second position in the sequon is the least subject to variations that would induce or abolish a sequon, as is expected due to the flexibility of residues at this position (can be any residue except P). Conversely, the first position requires an N, and is therefore expected to be the most subject to NLG-altering mutations. The third position, which can be occupied by either an S or a T, is neither as limited as the first position nor as flexible as the second, so we expect the frequency of sequon-altering variations to be between the corresponding frequencies for the first and second positions. When we average across all datasets, these trends do hold true; however, when we look at just the subset of somatic LOG mutations, we actually find that the third position is subject to a greater frequency of mutations than the first position (Fig. 3).
Proteome-wide pan-cancer analysis
Significant variations
Somatic-only LOG/GOG-causing variations are associated with 38 cancer types. For proteins either having signal peptides or annotated with keyword(s) “Secreted” or “Membrane,” we found 24 LOG-causing somatic-only variations in 24 sequons of 23 proteins and 41 GOG-causing somatic-only variations in 41 sequons of 40 proteins present in at least three cancer types (See Supplemental Tables S7–S10). If we only include high-confidence NLGs reported in databases, we find 13 LOG-causing somatic-only variations in 13 sequons of 12 proteins. Figure 4 shows a schematic of the localization of these proteins.
Interestingly, considering only the subset of high-confidence NLGs, there are 13 somatic-only LOG variations associated with three or more cancer types each (Table 4), two of which are associated with four or more cancer types each. Under these criteria, the most significant variations are Mast/stem cell growth factor receptor Kit (KIT, P10721) N486D and Thrombospondin type-1 domain-containing protein 7A (THSD7A, Q9UPZ6) T236M.
KIT is a transmembrane receptor tyrosine kinase commonly expressed in hematopoietic progenitor cells64. Inhibition of N-linked glycosylation of KIT has been reported to affect cellular signaling and cell-surface expression of KIT, inducing apoptosis in acute myeloid leukemia (AML)65, and glucose metabolism mediated by KIT in response to imatinib has been used to predict tumor sensitivity to the drug in gastrointestinal stromal tumors (GIST)66. Evidence suggests that stem cell factor, the activator of KIT’s autophosphorylation domain, only operates on a mature version of the protein with the appropriate N-linked glycosylation profile67. In our dataset, the N486D KIT variant was observed in bone cancer [DOID:184], rectum cancer [DOID:1993], colon cancer [DOID:219], and large intestine cancer [DOID:5672].
The status of THSD7A as an N-glycoprotein has been well documented, and inhibition of glycosylation associates with a lack of soluble THSD7A, the form known to promote endothelial cell migration in normal embryonic angiogenesis68. Interestingly, the involvement of all THSD7A LOG variations in cancer, including the T236M associated with four cancers (pharynx cancer [DOID:0060119], rectum cancer [DOID:1993], esophageal cancer [DOID:5041], and head and neck cancer [DOID:11934]), were all inferred from inclusion in ICGC, TCGA, COSMIC, and Intogen, and no literature citations were found for THSD7A variants involved in cancer, manually or through mapping to HGMD (Human Gene Mutation Database). However, THSD7A has been reported to map to the FRA7B common fragile site (CFS); CFS are known to correlate with recurrent breakpoints in some cancers69. While the lack of literature support for cancer involvement could indicate a lack of importance of THSD7A variation in cancer, this variant’s presence in four different cancers reported across four cancer databases (as listed above), in conjunction with its link to CFS, warrants additional examination. Thus, THSD7A represents a prime candidate for downstream validation of cancer involvement.
Because newly created NLGs are not expected to be experimentally verified glycosylation sites described in publications, we limit the scope of potential GOGs by relevant cellular compartment keyword and signal peptide annotation. Under these criteria, there are 41 somatic-only GOG-causing variants related to three or more cancers each (Table 5), six of which are related to four or more cancers each, and two related to five or more cancers each. These top two variations are Neurogenic locus notch homolog protein 1 (NOTCH1, P46531) A465T and Mucin-2 (MUC2, Q02817) T1750N.
NOTCH1 is a member of the highly conserved Notch signaling pathway, responsible for cell fate during development and homeostasis70,71. Extensive N- and O-linked glycosylation has been observed in the Notch extracellular domain71, where ligand binding induces conformational change and functional receptor activation72. A variety of roles have been described for Notch with respect to carcinogenesis and cancer stem cells73. We observed the NOTCH1 A465T variant in colon cancer [DOID:219], malignant glioma [DOID:3070], esophageal cancer [DOID:5041], head and neck cancer [DOID:11934], pharynx cancer [DOID:0060119], and brain cancer [DOID:1319].
Mucin-2 is known to be densely O-glycosylated74,75, and has been observed with downregulated expression in colorectal cancer76. In fact, expression of MUC2 has been associated with less aggressive forms of urothelial bladder cancer28. While there are many studies regarding MUC2 expression in cancer77 and its variable O-glycosylation patterns78, a direct link to N-glycosylation-altering-variation does not exist in current literature: this fact, combined with a potential gain of N-glycosylation variant in seven cancer types, warrants additional mechanistic exploration for the role of MUC2 in cancer. We observed the MUC2 T1750N variant in breast cancer [DOID:1612], liver cancer [DOID:3571], thyroid cancer [DOID:1781], ovarian cancer [DOID:2394], prostate cancer [DOID:10283], brain cancer [DOID:1319], and rectum cancer [DOID:1993].
Significant sequons
The protein with the highest number of somatic-only LOGs is Usherin (USH2A, O75445). 5,202 amino acids in length, Usherin has 27 LOG variations abolishing 25 high-confidence sequons. Similarly, the protein with the highest number of somatic-only GOGs is Titin (TTN, Q8WZ42) with 92 GOG predicted variations affecting 90 positions. However, this protein is annotated to be localized mostly to the cytoplasm or nucleus and does not have a signal peptide, implying that glycosylation is unlikely. Low-density lipoprotein receptor-related protein 1B (LRP1B, Q9NZR2) is a membrane protein with a signal peptide and has been observed to have recurrent mutations in urachal cancer79 and ovarian clear cell carcinoma80. LRP1B is commonly deleted across cancers81 and it has been proposed to be a tumor suppressor via modulation of the extracellular tumor environment in thyroid cancer cells82. In our dataset, LRP1B has 24 predicted somatic-only GOGs affecting 23 positions. Although no literature exists regarding glycosylation of LRP1B in cancer, its known participation in multiple cancers suggests that altered glycosylation of the protein could affect the course of the disease.
Significant proteins
If we divide the number of affected sequons per protein by the length of the corresponding protein, we can identify the proteins with the highest density of nsSNV-affected NLGs per unit length. Considering only the proteins that are annotated with keywords “secreted” or “membrane,” in the LOG set, the top-ranked protein by density is Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM7, Q14002) with five mutations abolishing sequons at five distinct positions. CEACAM7 belongs to the immunoglobulin superfamily, specifically to the carcinoembryonic antigen (CEA) gene family, and normally functions in regulation of cellular differentiation83. UniProtKB reports CEACAM7 to be strongly downregulated in colonic adenocarcinomas, and it has been reported to be a predictive marker for rectal cancer recurrence84. Among the five somatic-only variations leading to LOG on CEACAM7 three are observed in skin cancer, the other two in prostate and pharynx cancers, independently. Although this protein only has five LOG variants, it is a relatively small protein (265 residues in length) resulting in a density of one LOG per every 53 residues. Another CEA family gene, Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM 5, P06731), also has one of the highest densities of somatic-only LOG variants. CEACAM5 has 12 variants occurring at 11 positions, for a density of one LOG per every 64 residues. Six of the variants are observed in skin cancer, with others reported in colon, kidney, uterine, ovarian, and hematologic cancers. In the somatic-only GOG set, the top-ranked protein by density is Cellular tumor antigen p53 (TP53, P04637), with 15 GOG variants for an average of one per every 27 residues. While mutation of TP53 is well-characterized to interrupt its normal transcription factor function and lead to the development of many different cancers85, its cytoplasmic and nuclear localizations, as well as lack of signal peptide, make it an unlikely candidate for biologically viable N-glycosylation. Epididymal-specific lipocalin-8 (LCN8, Q6JVE9) is a secreted protein with a signal peptide with a density of one GOG variant for every 59 residues. Its three GOG variants are reported in liver and skin cancer datasets, but there is no current literature linking this gene to cancer.
Pathway and biomarker analysis summary
LOG/GOG-cancer associations
Of 8,894 distinct LOG somatic-only variants, only 20 were indicated in publications reported from HGMD (Human Gene Mutation Database, June 2016 release)86, only one of which is directly cancer-related (CREBBP Q92793 N1978D; ovarian cancer) and another nine with cancer-related syndromes. Similarly, of 12,939 distinct GOG somatic-only variants, only 16 overlap with HGMD disease annotations, none of which are directly cancer-related, and two of which confer increased risk of certain cancers. With respect to germline variants mapping to the LOG and GOG subsets, we see 349 LOG-associated germline variants and 436 GOG-associated germline variants mapping to HGMD mutations, out of a total of 40,365 HGMD mutations found in the entire nonsynonymous germline dataset retrieved from dbSNP. We hypothesize that mutations already associated with any disease (not restricted to cancer alone) identified in our somatic-only datasets could become high-value biomarkers for different cancer types based on existing literature evidence for functional disruption. At the same time, the minimal overlap between the HGMD dataset and our somatic-only findings suggests enormous potential for validation of as yet unpublished cancer-associated LOG/GOG-causing variants. (See Supplemental Tables S7 and S8 for more information.)
Enrichment analysis
The top five canonical MetaCore™ pathways enriched across the set of all cancer-associated NLG variations (including both LOG- and GOG-causing variants) include muscle contraction GPCRs in the regulation of smooth muscle tone (P = 2.120e-09), signal transduction mTORC2 downstream signaling (P = 1.058e-8), development regulation of epithelial-to-mesenchymal transition (EMT) (P = 1.477e-8), breast cancer (general schema) (P = 9.996e-8), and nociception nociceptin receptor signaling (P = 1.259e-7). Interestingly, protein folding and maturation POMC processing (P = 2.373e-10) is strongly enriched in the GOG dataset, including 19/30 associated pathway genes, but having only two genes in common with the corresponding LOG dataset and no additional genes unique to the LOG dataset. This implies a strong bias for this pathway in potential pathogenesis of cancer associated with GOG mutations. The top five pathways for the GOG somatic variants (considered independently of any potential LOG overlap, ranked by statistical significance) are cytoskeleton remodeling_TGF, WNT and cytoskeletal remodeling (P = 4.857e-12), cytoskeleton remodeling_cytoskeleton remodeling (P = 4.778e-11), ovarian cancer (main signaling cascades) (P = 1.668e-10), cell adhesion_chemokines and adhesion (P = 9.465e-10), and development_regulation of cytoskeleton proteins in oligodendrocyte differentiation and myelination (P = 1.166e-9). Similarly, the top five LOG somatic variants include signal transduction_mTORC2 downstream signaling (P = 4.862e-11), development_regulation of epithelial-to-mesenchymal transition (EMT) (P = 1.985e-8), cell adhesion_histamine H1 receptor signaling in the interruption of cell barrier integrity (P = 2.192e-8), cell adhesion_cadherin-mediated cell adhesion (P = 4.214e-8), and some pathways of EMT in cancer cells (P = 1.002e-7).
Discussion
The primary aims of this study were to: (1) provide a comprehensive view of protein N-linked glycosylation sequons within the human genome; and (2) compare cancer-centric nsSNVs and germline SNPs with respect to their relative impact on protein N-linked glycosylation sequons. Despite the in silico nature of this work, the consideration of existing experimental literature and functional annotations as evidence supporting the likelihood of possible glycosylation at various sites will allow downstream users of these findings to prioritize sites for additional study and to provide experimental validation that is currently lacking. With this goal in mind, we aimed to delineate which sites were verified sites of glycosylation and which were not, further classifying un-verified sites as possible and probable based on the evidence available for each site. To this end, we provided a comprehensive survey of human NLGs including up-to-date values for: (1) the number of experimentally verified human N-linked glycosylation sites (16,253, or 27.4% of all NLGs); (2) the proportion of human proteins containing at least one NLG (75.8%, or 15,318 of 20,199 total proteins); and (3) the average number of NLGs per human protein (3.9).
We further aimed to associate possible loss or gain of glycosylation with the development of cancer by looking at the subset of variants expected to cause loss or gain of an NLG in somatic-only cancer variant calls. While loss or creation of an NLG does not necessarily impact N-glycosylation, we used additional functional information (including presence of signal peptides and subcellular locations), existing literature regarding role in N-glycosylation, and structural modeling predictions to devise a high-confidence criterion for likelihood of biologically plausible glycosylation. Even if glycosylation has been observed at a given site, effects on so-called “normal” glycosylation at a single site does not guarantee a functional consequence. However, combining information regarding the pervasiveness of independent variants across multiple cancers with evidence supporting plausible glycosylation allows us to rank variants. We have provided lists of variants (both LOG and GOG) meeting at least one high-confidence requirement for glycosylation and appearing in at least three cancers (Supplemental Tables S9 and S10). We suggest prioritizing these variants for additional study including possible experimental validation of N-glycosylation. While the change of the glycosylation status itself may not be pathogenic, the complexity of N-glycosylation and its potential impact on a number of factors may warrant multi-faceted exploration to determine whether a possible association with cancer could be directly explained by simple variation, loss or gain of glycosylation, or downstream effects of altered glycosylation. We expect that these cancer-associated, high-confidence, LOG/GOG-causing variants may lead to biomarker development and help to better elucidate the role of glycosylation in carcinogenesis.
Materials and Methods
Data retrieval and integration
Somatic nsSNVs were collected from COSMIC (CosmicCompleteExport_v73), IntOGen (Release-2014-12), ICGC (Data Release v0.10a), TCGA (Release-2015-01-27), ClinVar (ClinVarFullRelease_2015-02-05), and literature mining methods87; germline SNPs were collected from dbSNP (Human Build 149). Cancer-related somatic nsSNVs were integrated using the previously described BioMuta workflow23 for pan-cancer analysis such that all cancer types were mapped to DO terms by corresponding disease ontology IDs (DOIDs)62. Annotated sequence functional site data for N-linked glycosylation retrieved from UniProtKB/Swiss-Prot (release-2015_01), HPRD 9.088, dbPTM 3.089, neXtProt release 2017-01-2190 and NCBI-CDD (2015_Jan)91 were treated as high-confidence results. For the purpose of this paper, high-confidence NLGs are those which are annotated as a glycosylation site in a UniProt FT line associated with a PMID or manual assertion, or reported directly from the other databases listed above.
Identification of real and predicted human NLGs
The identification of all possible human NX(S/T) (X! = P) sequons was performed by three methods. The reference UniProtKB/Swiss-Prot human proteome was obtained in January 2015 from the UniProt ftp site at ftp://ftp.uniprot.org/pub/databases/uniprot/previous_releases/release-2015_01/knowledgebase/knowledgebase2015_01.tar.gz, and annotated N-linked glycosylation sites were retrieved from this set of protein entries. Additionally, the NetNGlyc-1.0 [http://www.cbs.dtu.dk/services/NetNGlyc/] tool was used to predict possible N-linked glycosylation sites. Finally, all NX(S/T) (X! = P) sequons in the human proteome were identified by searching all human protein sequences using custom Python scripting.
Calculating frequency of human NLG occurrence and occupancy rate
The proportion of NLG-containing human proteins was reported as the number of human proteins in UniProtKB/Swiss-Prot with at least one NLG divided by the total number of human proteins in UniProtKB/Swiss-Prot. Frequency of NLGs was calculated as the total number of human NLGs divided by the total number of human proteins in UniProtKB/Swiss-Prot. Occupancy was calculated as the number of real N-linked glycosylation sites divided by the number of human NLGs in UniProtKB/Swiss-Prot.
Mapping NLGs to variations and reporting of LOG/GOG
Each potential NLG was mapped to variation datasets and reported as LOG or GOG. An altered NLG was considered a LOG if the altered version had an N abolished at the first position, an S or T abolished at the third position, or a newly generated P at the second position. Following the rationale of our previous study55, variants that alter T to S (or S to T) at the third position were not considered to functionally affect NLGs. An altered NLG was considered a GOG if the altered version contained a newly generated N at the first position, a newly generated S or T at the third position, or an abolished P within a normal NPS/T subsequence. Variants were also mapped to the human proteome and analyzed using NetNGlyc to predict additional GOGs. Figure 5 shows the workflow for the LOG/GOG identification and variation mapping processes. It should be noted that the NetNGlyc tool may predict a GOG by modification of a residue outside the sequon and may also predict GOG including P at the middle sequon position: the number of GOGs suggested as candidates for further analysis was based on overlap between simple mapping results and NetNGlyc predictions.
LOG and GOG identification and pan-cancer analysis
Significant variations, sequons, and proteins were identified according to the mapping results for LOG and GOG, especially for variations observed in multiple types of cancer. To assess the potential enrichment of a given feature and discrepancy of occurrence of that feature between datasets, a binomial test was used as described in an earlier study92. For example, to determine the P-value for significance of abolished sequons, the total number of abolished sequons across all methods was used to determine the expected rate of abolished sequons; this rate was then compared to the observed rate of abolished sequons for a given method. To compare somatic and germline variations, we used the somatic-only nsSNVs and germline SNPs and excluded any overlapping variations between the two datasets. Numbers of associated cancer types were counted by summing distinct DOIDs annotated at the variant level. Note that DOID mapping in BioMuta was done only for the subset of cancer-related DOIDs (CDO slim)62. Since some samples are of a particular cellular subtype, they may automatically map to multiple terms designating tissue-level and cell-level specificity separately.
HGMD comparison
Our set of cancer-related, NLG-impacting variants was compared to variants in the Human Gene Mutation Database (HGMD, HGMD_Professional_2016.2)93. Because HGMD includes only published gene lesions, any identified nsSNV-affected LOG/GOG not cross-referenced by HGMD represented possible novel findings.
Enrichment analysis
Enrichment of pathways was analyzed with MetaCore™ (https://portal.genego.com).
Data availability
All data generated or analyzed during this study are available as supplemental tables accompanying this publication and can be browsed and downloaded from https://hive.biochemistry.gwu.edu/kbdata/view/loss_and_gain_of_n_linked_glycosylation_sequons_in_cancer by selecting tables with prefix “NLGPaper” from the dropdown menu.
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Acknowledgements
This work is funded in part by National Cancer Institute (NCI) (UO1 CA168926, EDRN Associate Member agreement #156620 and The McCormick Genomic and Proteomic Center, Department of Biochemistry and Molecular Medicine, The George Washington University). Additionally, the authors wish to acknowledge John Torcivia and Ting-Chia Chang for advice on data integration and Krista Smith and Amanda Bell for editorial critique. Hayley Dingerdissen is a doctoral candidate in the Molecular Oncology Track of the Institute for Biomedical Sciences at the George Washington University. This work is from a dissertation to be presented to the above program in partial fulfillment of the requirements for the Ph.D. degree.
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R.M., R.G. planned experiments; Y.F., Y.H., H.M.D. performed experiments; Y.F., Y.H., H.M.D. analysed data; C.Y., Y.P. contributed reagents or other essential material; Y.F., H.M.D. wrote the manuscript. All authors reviewed the manuscript.
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Fan, Y., Hu, Y., Yan, C. et al. Loss and gain of N-linked glycosylation sequons due to single-nucleotide variation in cancer. Sci Rep 8, 4322 (2018). https://doi.org/10.1038/s41598-018-22345-2
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DOI: https://doi.org/10.1038/s41598-018-22345-2
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