Abstract
Dihydrodipicolinate reductase (DHDPR) is a key enzyme in the diaminopimelate- and lysine-synthesis pathways that reduces DHDP to tetrahydrodipicolinate. Although DHDPR uses both NADPH and NADH as a cofactor, the structural basis for cofactor specificity and preference remains unclear. Here, we report that Paenisporosarcina sp. TG-14 PaDHDPR has a strong preference for NADPH over NADH, as determined by isothermal titration calorimetry and enzymatic activity assays. We determined the crystal structures of PaDHDPR alone, with its competitive inhibitor (dipicolinate), and the ternary complex of the enzyme with dipicolinate and NADPH, with results showing that only the ternary complex had a fully closed conformation and suggesting that binding of both substrate and nucleotide cofactor is required for enzymatic activity. Moreover, NADPH binding induced local conformational changes in the N-terminal long loop (residues 34–59) of PaDHDPR, as the His35 and Lys36 residues in this loop interacted with the 2′-phosphate group of NADPH, possibly accounting for the strong preference of PaDHDPR for NADPH. Mutation of these residues revealed reduced NADPH binding and enzymatic activity, confirming their importance in NADPH binding. These findings provide insight into the mechanism of action and cofactor selectivity of this important bacterial enzyme.
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Introduction
Sporulation is a major survival strategy for certain Gram-positive bacteria, including Bacillus and Clostridium species1,2,3. Endospore formation begins with asymmetrical cell division, followed by the formation of an outer cell wall comprising various coat proteins that enable bacteria to resist harsh environmental stress. Over 500 genes are involved in sporulation4,5,6, and dihydrodipicolinate (DHDP) is a key compound in bacterial sporulation that acts as a precursor for two important pathways: the dipicolinate (DPA)-synthesis pathway and the diaminopimelate (DAP)- and lysine-synthesis pathway7,8,9,10. DPA is a major component of bacterial endospores (5–15% of the total mass) that forms a complex with calcium ions and causes spore dehydration by binding free water molecules11. The calcium-DPA complex also protects bacterial DNA against environmental stresses by inserting itself between nucleotides12,13. On the other hand, DAP is a component of peptidoglycan in the cell wall of many bacteria that forms cross-links with and thereby confers rigidity to the bacterial cell wall14,15,16. These two pathways mutually regulate cellular activity through a feedback mechanism, with DPA inhibiting lysine synthesis17,18. Moreover, enzymes in these two pathways are potential targets for the design of antibiotics against spore-forming pathogens.
DHDP reductase (DHDPR; dapB) is a branching enzyme associated with two described pathways and that reduces DHDP to tetrahydroDPA using NAD(P)H as a cofactor19. To date, crystal structures of DHDPR from Escherichia coli, Mycobacterium tuberculosis, Thermotoga maritima, Staphylococcus aureus, Bartonella henselae, and Corynebacterium glutamicum have been resolved20,21,22,23,24,25. DHDPR has two domains, including an N-terminal nucleotide-binding domain and a C-terminal domain for substrate binding and tetramerization20. Cofactor and substrate binding induce the switch between the open and closed conformations of these domains. The preference of DHDPR for NADH or NADPH varies across species, with orthologues in E. coli (EcDHDPR) and M. tuberculosis (MtDHDPR) preferring NADH over NADPH, whereas the opposite is true for the T. maritima and S. aureus orthologues (TmDHDPR and SaDHDPR, respectively)21,23,26,27,28. However, the mechanisms associated with DHDPR discrimination between NADH and NADPH and the region and residues responsible for cofactor selectivity remain unknown.
To address these issues, we determined the structure and characterized the biochemical properties of DHDPR from the psychrophilic bacterium Paenisporosarcina sp. TG-14 (PaDHDPR) isolated from sediment-laden, stratified basal ice from Taylor glacier, McMurdo dry valley, Antarctica29. This Gram-positive species is reportedly capable of surviving millions of years in the spore state30,31,32. The results of isothermal titration calorimetry (ITC) and enzyme activity assays demonstrated that PaDHDPR has a strong preference for NADPH as a cofactor. Additionally, we determined the crystal structures of unliganded PaDHDPR and the enzyme in complex with DPA alone or with NADPH and performed mutagenesis studies in order to gain insight into the structural basis for this cofactor preference.
Results and Discussion
Biochemical characterization of PaDHDPR
DHDPR uses nucleotides as an electron source (Fig. 1), with nucleotides bound to the positively charged nucleotide-binding site at the N-terminus serving as a cofactor. The kcat value for PaDHDPR was 7.7-fold higher in the presence of NADPH, whereas the Km was 2-fold lower for NADPH than that for NADH, demonstrating that the enzyme strongly prefers the phosphorylated form of the molecule (NADPH). To confirm this result, we evaluated the binding affinities of NADH and NADPH by ITC (Fig. 2A,B). Titration experiments were performed with 80 μM PaDHDPR in the sample cell and 1 mM NADH or NADPH. NADH did not bind to PaDHDPR; however, PaDHDPR had strong affinity for NADPH, with a Kd value of 37 μM (Fig. 2A), which was slightly lower than those reported for EcDHDPR (2.1 μM) and SaDHDPR (0.9 μM)23,26,28.
TmDHDPR and SaDHDPR are inhibited by high substrate concentrations27,28. When l-aspartate-semialdehyde (ASA) concentrations were varied using NADPH as a cofactor, the kcat and Km of PaDHDPR were 52 ± 1.1 s−1 and 12 ± 1.6 μM, respectively (Supplementary Table S1). Notably, PaDHDPR showed no inhibition at concentrations up to 0.2 mM ASA, which was consistent with results for EcDHDPR and MtDHDPR23,26. This suggests that the lysine-synthesis pathway is differentially regulated depending on the species. We then investigated the melting temperature, pH dependence, and optimal temperature of PaDHDPR using NADPH as a cofactor (Fig. 2A,D). The melting temperature of PaDHDPR was 52.7 °C (Supplementary Fig. S1), and maximal activity was observed at a pH range of 7.5 to 8.0, which is similar to the optimal pH of the Bacillus homolog, and 40 °C33. Although the recombinant PaDHDPR enzyme did not exhibit a low optimal temperature expected for enzymes isolated from psychrophilic bacteria, >60% of the maximal activity was retained at 20 °C. Some multi-subunit enzymes show characteristics of thermal stability similar to mesophilic homologues, despite their having been isolated from psychrophilic organisms34,35. A previous study showed that maize DHDPR is highly sensitive to heat, and that its activity is sharply reduced under such conditions (a 69% loss of activity at 45 °C)36. Recent studies also suggested that plant DHDPRs might have different quaternary structure as a dimer according to investigation of Arabidopsis thaliana DHDPR37. These findings suggest that bacterial DHDPRs are more heat stable than plant DHDPRs due to their forming a tetramer in solution (Supplementary Fig. S1).
Crystal structure of PaDHDPR
The crystal structures of apo PaDHDPR (without the cofactor) and the DPA- and NADPH/DPA-bound forms of the enzyme were determined at 1.8 Å, 1.8 Å, and 2.1 Å resolutions, respectively (Fig. 3). Despite PaDHDPR sharing 42% sequence identity with DHDPR from Anabaena variabilis [AvDHDPR; Protein Data Bank (PDB): 5KT0, unpublished], molecular replacement did not yield a solution. Therefore, an initial partial structure of PaDHDPR was determined at 2.0 Å by single-wavelength anomalous dispersion (SAD) based on the anomalous scattering of Se atoms38. The final 1.8 Å high-resolution native model of PaDHDPR was refined and obtained by molecular replacement using the initial SAD-phasing model. Both were crystallized in the hexagonal space group P6422, with one molecule in the asymmetric unit. PaDHDPR tetramerization was observed by analytical centrifugation and through generation of a crystallographic symmetry mate (Fig. 3B and Supplementary Fig. S2). The final model of the apo PaDHDPR monomer included 264 amino acid residues and 296 water molecules, with an overall topology comprising eight α-helices and 11 β-strands. DynDom analysis of PaDHDPR structures show distinct N- and C-terminal domains linked by two hinge regions (residues 129–133 and 233–235), with a 32.3° rotation angle and 2.1-Å translational movement39. The N-terminal domain (residues 1–127 and 235–264) responsible for cofactor binding comprised a parallel six-stranded β-sheet surrounded by six α-helices. The C-terminal domain (residues 128–234) responsible for substrate binding and tetramer formation included a five-stranded β-sheet and two α-helices. In the tetramer, each C-terminal domain formed a central 20-stranded β-barrel structure surrounded by eight α-helices. Each N-terminal domain was located outside of the tetrameric core structure formed by the four C-terminal domains. These structural features are similar to those reported for other DHDPR tetramers. A structural homology search using the DaliLite server (https://www.ebi.ac.uk/Tools/structure/dalilite/) revealed that DHDPR from various species had high Z scores, with the highest score observed for AvDHDPR (29.7), followed by NADH-bound MtDHDPR (PDB: 1YL7; 28.8) and DHDPR from C. glutamicum (PDB: 5EER; 28.5)25,40,41.
The crystal structure of DPA-bound PaDHDPR was also obtained in the hexagonal space group P6422 and solved by molecular replacement using the apo form as a search model. Electron density corresponding to DPA was observed in the substrate-binding site of the C-terminal domain. Structural superposition of apo and DPA-bound PaDHDPR revealed that the two conformations were highly similar, with a root-mean-square deviation of 0.54 Å (over 244 Cα atoms) (Fig. 4A). This suggests that DPA alone cannot induce domain closure of PaDHDPR. The structure of the ternary complex (NADPH/DPA-bound PaDHDPR) was determined by molecular replacement using separate domain-structure models of apo PaDHDPR. Clear electron density was observed in the putative N-terminal nucleotide-binding site that showed good correspondence with NADPH. The electron density of DPA was also observed adjacent to the NADPH-binding site. Comparison of the overall structures of apo and NADPH/DPA-bound PaDHDPR showed a root-mean-square deviation of 3.6 Å (over 244 Cα atoms) due to domain motion initiated upon NADPH and DPA binding. By contrast, structural superposition of each domain of apo and NADPH/DPA-bound PaDHDPR revealed relatively small differences, with root-mean-square deviations of 0.54 Å and 0.24 Å for the N- and C-terminal domains, respectively (over 125 and 84 Cα atoms, respectively) (Fig. 4B,C).
Open and closed conformations of PaDHDPR
The N-terminal nucleotide-binding and C-terminal substrate-binding domains of PaDHDPR are linked by two flexible hinge regions that mediate the opening and closing of the enzyme. The apo and DPA-bound forms of PaDHDPR were in an open state (Fig. 4A), whereas the DPA/NADPH-bound enzyme was in a closed state (Fig. 4B). Similarly, the apo (PDB: 5TEK, unpublished) and nucleotide-bound (PDB: 1Y17) forms of TmDHDPR were both in the open state (Fig. 4D)40, whereas the substrate- and nucleotide-bound ternary complexes existed in closed states (Fig. 4E). Therefore, DHDPR binding of both substrate and nucleotide is required to induce a conformational switch to the closed state for enzymatic activity. It should be noted that an NADH-bound MtDHDPR structure showed a partially closed conformation, with the authors speculating that this unexpected closed structure might represent a snapshot of the equilibrium state between open and closed forms awaiting further substrate binding, and that subsequent substrate binding might induce the fully closed conformation21.
DPA binding in PaDHDPR induced a small change in the α5–β5 loop region (Fig. 4A); however, it is unlikely that the change was due to DPA binding alone, as the distance between the loop and DPA is approximately 9 Å. In the closed state of PaDHDPR, the interaction surface between the N- and C-terminal domains can be divided into two components: one for the interaction mediated by NADPH and DPA, and another for the interaction mediated by the α7 helix (Fig. 4F). Specifically, the NZ atom of Lys12 interacts with the NO1 atom of the adenine phosphate of NADPH, as well as with the OE1 atom of Gln157. The Thr100 residue interacts with NADPH and DPA and also forms a hydrogen bond with Asp160. The OG1 atom of Thr165 forms a hydrogen bond with the ND2 atom of Asn126, as well as with the O3 atom of DPA. Lys168 forms a salt bridge with Glu261, whereas Lys167 interacts with the main-chain O atom of Tyr258. Gln171 interacts with the side chain of Tyr258 and Asp104.
The NADPH-binding site of PaDHDPR
The N-terminal domain of PaDHDPR contains the sequence G-x-x-G-x-x-G (Gly8, Pro9, Arg10, Gly11, Lys12, Met13, and Gly14), which is a conserved nucleotide-binding motif in DHDPRs. Additionally, the nucleotide-binding site of PaDHDPR contains positive charges that match the negative charges of NADPH phosphates. Specifically, the O1A of adenine phosphate interacts with the NZ of Lys12, the C2 phosphate group of the adenine ribose interacts with the Arg10, His35, and Lys36 side chains, the O2X of adenine phosphate forms hydrogen bonds with the NH2 of Arg10 and the NZ of Lys36, the O3X of adenine phosphate forms hydrogen bonds with the NE2 atom of His35, the O2D and O3D of the nicotinamide ribose interact with OG1 of Thr100, and the main-chain oxygen atoms of Ala124 and Gly98 form hydrogen bonds with the N7N of the nicotinamide amide group (Fig. 5B,C). Structural superposition of the N-terminal domains of apo and NADPH/DPA-bound PaDHDPR reveal conformational changes in the region including residues 34 through 59 and resulting from NADPH binding (Fig. 4B,C). In the apo form, this loop region shows a relatively high B-factor as compared with other regions. Additionally, the Gln80 side chain was flipped outward, and changes in Thr100 and Thr77 position were observed following NADPH binding (Fig. 5D).
Comparison of NADPH-binding modes among various DHDPRs
Previous studies show that the N-terminal long-loop (NLL)-region residues are important for cofactor specificity in DHDPRs. EcDHDPR can use both NADH and NADPH, although it exhibits a slight preference for NADH. In the NADH-bound EcDHDPR structure, Glu38 in the NLL region interacts with the 2′ and 3′ hydroxyl groups of the NADH adenosyl ribose. On the other hand, Arg39 is involved in NADPH binding to EcDHDPR through direct interaction with the adenine phosphate of NADPH. Therefore, these two residues in the NLL region might confer EcDHDPR with dual specificity26. It is also possible that the Lys or Arg residues in the NLL region might play critical roles in NADPH preference in DHDPRs, such as TmDHDPR and SaDHDPR.
Structural comparison of the N-terminal domains of NADPH/DPA-bound PaDHDPR and NADPH-bound C. glutamicum (Cg)DHDPR (PDB 5EES; sequence identity: 44%) revealed two key differences in the NADPH-binding mode (Fig. 6A)25. CgDHDPR lacks residues equivalent to His35 and Lys36 in PaDHDPR due to a very short NLL region (Fig. 3C). Additionally, Lys11, which is equivalent to Arg10 in PaDHDPR, forms a salt bridge with Glu33 instead of interacting with the adenine phosphate group of NADPH. These structural features explain the promiscuity of CgDHDPR in regard to cofactor preference between NADH and NADPH.
B. henselae (Bh)DHDPR (PDB: 3IJP; sequence identity: 27%) also contains an NLL region, but its interaction with the adenine phosphate of NADPH differs significantly from that of PaDHDPR (Fig. 6B) due to differences in local structure and amino acid sequence24. Arg34 of BhDHDPR, which is the equivalent to His35 in PaDHDPR, forms a charged interaction with the adenine phosphate of NADPH. However, Lys35 (Lys36 in PaDHDPR) in the NLL region is exposed to solvent in the absence of any specific interaction with bound NADPH. Moreover, Asn9 in BhDHDPR and located in the β1–α1 loop interacts with the ribose AO3 of NADPH. Based on these observations, BhDHDPR might harbour a weak cofactor preference for NADPH over NADH.
Our comparative structural analyses demonstrate that PaDHDPR has more specific interactions with the adenine phosphate of NADPH. The ITC analysis confirmed that H35A mutation induced complete loss of binding affinity and K36A mutants had approximately 6.4 folds reduced affinity (Kd value of 236 µM) for NADPH compared with that of wild-type PaDHDPR (Kd value of 37 µM) (Fig. 6C). Therefore, our findings suggested that residues located in the NLL region of PaDHDPR engage in specific interactions with NADPH. We speculated that DHDPR preference for a given form of NAD(P)H is determined by the combination of various residues in this region. Notably, our sequence-alignment results showed that the residues therein vary considerably across species (Fig. 3C), with TmDHDPR, which has a strong preference for NADPH, having a very short NLL region22. Additional biochemical and structural data are needed in order to determine the cofactor preference of DHDPRs based solely on sequence information.
The substrate-binding site of PaDHDPR
To investigate the substrate-binding mechanism of PaDHDPR, we determined the structures of DPA- and DPA/NADPH-bound forms of the enzyme (Fig. 7A). DPA is a competitive inhibitor of DHDPR and has a structure similar to DHDP, a PaDHDPR substrate. In the ternary complex, the aromatic ring of DPA faces the nicotinamide ring of NADPH and separated by a distance of 3.4 Å to 4 Å. We also observed that the carboxyl group O3 of DPA interacts with the OG1 of Thr165 and the main-chain O of Pro125, and that the carboxyl group O1 on the opposite side of the of DPA molecule interacts with the NE2 of His155. The NE of Arg236 interacts with the O1 of DPA, with this interaction mediated by a water molecule. Lys158 is involved in substrate polarization and enamine stabilization26. Here, we found here that Lys158 directly interacts with the N1 of DPA, with its importance for enzymatic activity confirmed by enzyme inactivity following introduction of a K158A mutation (Fig. 7B).
Conclusion
In this study, we performed biochemical and structural characterization of PaDHDPR and provided the first report on a DHDPR from psychrophilic bacteria. Although PaDHDPR showed maximal activity at 40 °C, it retained >40% of its activity at 10 °C. Comparative structural analyses suggested that transition between the open and closed conformations in DHDPRs is determined by the binding of both substrate and nucleotide, given that only the DPA/NADPH-bound form of PaDHDPR showed a closed conformation. Moreover, NADPH binding induced a dramatic conformational change in the NLL region of PaDHDPR, with His35 and Lys36 in this region interacting with the adenine phosphate of NADPH and critical for nucleotide binding. The activity of NADH-bound PaDHDPR was ~20% to 30% lower than that of the NADPH-bound enzyme, indicating that PaDHDPR prefers NADPH as a cofactor, but can still use NADH despite a very low affinity. Interestingly, the K36A mutant exhibited reduced NADPH-binding affinity according to ITC results, but exhibited slightly increased activity relative to that of the wild-type variant according to activity assays. This might be a consequence of the DHDP substrate promoting NADPH binding in the K36A mutant. In our DPA/NADPH-bound complex structure, the aromatic ring of DPA forms π-π stacking interaction with the nicotinamide ring of NADPH (Fig. 7A). It is also possible that the K36A mutant has a more flexible NLL region that might allow a more rapid transition between the open and closed conformations during enzyme activity. Similarly, Asp37 located in the NLL region is important for loop stabilization, and the D37A mutant also showed a slight increase in enzyme activity as compared with that of wild-type PaDHDPR (Fig. 7B). Therefore, we speculated that destabilization of the NLL structure might result in elevated PaDHDPR activity (Supplementary Fig. S3). These findings provide a basis for the development of drugs against spore-forming bacteria that act by targeting the lysine-synthesis pathway.
Methods
PaDHDPR cloning, expression, and purification
The gene encoding DHDPR (residues 1–264) was amplified by polymerase chain reaction (PCR) from the genomic DNA of Paenisporosarcina sp. TG-14 using PrimeSTAR HS DNA polymerase (Takara Bio, Otsu, Japan) and the primers 5′-CGATAACATATGATGATTCGAGTTGCGATTG-3′ (forward) and 5′-CGATAACTCGAGTTATAAAATATTTTCTAG-3′ (reverse). The DNA fragment was cloned into the NdeI and XhoI sites of the pET-28a vector (Novagen, Madison, WI, USA). The construct, which introduced a cleavable N-terminal His tag, was transformed into E. coli BL21(DE3) competent cells for protein expression. The cells were cultured at 37 °C in 4 L of Luria-Bertani (LB) medium containing 50 µg ml−1 kanamycin until the optical density at 600 nm (OD600) was ~0.6. PaDHDPR expression was induced overnight at 25 °C by 0.4 mM isopropyl-1-thio-β-d-galactopyranoside (IPTG). The cells were harvested by centrifugation and lysed by sonication in lysis buffer comprising 50 mM sodium phosphate, 300 mM NaCl, and 5 mM imidazole (pH 8.0) with 0.2 mg ml−1 lysozyme. The debris was removed by centrifugation at 16,000 rpm for 1 h at 4 °C. The supernatant was applied to an Ni-nitroloacetic acid affinity column (Qiagen, Hilden, Germany) pre-equilibrated with lysis buffer and then washed with wash buffer comprising 50 mM sodium phosphate, 300 mM NaCl, and 20 mM imidazole (pH 8.0). The target protein was eluted with elution buffer comprising 50 mM sodium phosphate, 300 mM NaCl, and 300 mM imidazole (pH 8.0) and concentrated using Amicon ultracentrifugal filters (Ultracel-3K; Millipore, Darmstadt, Germany). The His tag of the purified protein was removed by thrombin treatment, and the protein was applied to a Superdex 200 column (GE Healthcare, Piscataway, NJ, USA) pre-equilibrated with buffer comprising 50 mM Tris-HCl (pH 8.0) and 150 mM NaCl. Peak fractions containing PaDHDPR were collected and concentrated.
Selenomethionine (SeMet)-labelled PaDHDPR was expressed in E. coli BL21(DE3) competent cells. The transformed cells were grown at 37 °C in 2 l SeMet medium (Molecular Dimensions, Maumee, OH, USA) containing 50 µg ml−1 kanamycin until the OD600 was ~0.6. Protein expression was induced overnight at 25 °C with 0.4 mM IPTG. SeMet-labeled PaDHDPR was purified in the same manner as native PaDHDPR.
Enzymatic activity assay
DHDPR activity was evaluated at 25 °C via a coupled reaction with DHDPS (dapA) to form the substrate, as previously described42. PaDHDPS was cloned, expressed, and purified in the same manner as PaDHDPR. The standard reaction mixture contained 100 mM HEPES (pH 7.5), 0.1 mM NAD(P)H, 1 mM sodium pyruvate, 0.1 mM ASA, an excess of PaDHDPS (25 μg), and PaDHDPR. After 1 min of incubation with PaDHDPS for complete conversion of ASA to DHDP, the reaction was initiated by adding PaDHDPR. DHDPR activity was measured by monitoring the decrease in absorbance of NAD(P)H at 340 nm (molecular extinction coefficient = 6.22 mM−1 cm−1) with a spectrophotometer (UV-1800; Shimadzu, Kyoto, Japan). Kinetic parameters were determined by varying the concentration of NAD(P)H or ASA. When DHDPR was assayed with known concentrations of ASA, the concentration of NADP or NADPH varied. The kinetic parameters for ASA were determined by varying ASA concentration and maintaining a fixed concentration of NADPH. Enzyme Km and kcat were estimated by fitting the initial rate data to the Michaelis-Menten equation by nonlinear regression using Prism software (v.5; GraphPad, Inc., San Diego, CA, USA).
Optimal pH and temperature
The optimal pH was determined by measuring enzymatic activity at various pH values ranging from 5.0 to 9.0 under standard assay conditions. Various buffers were used, including 100 mM sodium acetate (pH 5.0–6.5), 100 mM HEPES (pH 6.5–8.5), and 100 mM Tris-HCl (pH 8.0–9.0). The optimal temperature was determined by performing the assay at temperatures ranging from 5 °C to 60 °C. Maximal enzymatic activity was set as 100% relative activity.
ITC
ITC experiments were performed using a MicroCal Auto-iTC200 instrument (GE Healthcare) at the Korea Basic Science Institute (Daejeon, Korea). Protein and ligand solutions were prepared in buffer comprising 50 mM Tris-HCl and 150 mM NaCl (pH 8.0), and all titrations were performed at 25 °C. The sample cell containing 80 μM PaDHDPR was titrated with 1 mM NADH or NADPH in the syringe. Data were fitted to a one-site binding model using MicroCal ORIGIN software, which was provided with the instrument.
Crystallization and data collection
Purified native PaDHDPR protein was concentrated to 215.95 mg ml−1. Initial crystallization screening was performed at 293 K in 96-well crystallization plates (Emerald Bio, Bainbridge Island, WA, USA) using a crystallization robotic nanoliter sitting-drop system (Mosquito; TTP Labtech, Cambridge, MA, USA) and a commercially available screening kit. The drops contained 300 nl each of protein and reservoir solutions and were equilibrated against 80 μl reservoir solution. Crystals of PaDHDPR were grown under conditions of 30% (v/v) polyethylene glycol (PEG)400, 100 mM Tris-HCl (pH 8.5), and 200 mM MgCl2 (Wizard Classic #C1). Crystals of SeMet-labeled PaDHDPR were obtained in the same manner under the same conditions. Co-crystals of DPA-bound PaDHDPR with 10 mM DPA were obtained from the condition of 30% (v/v) PEG 400, 100 mM HEPES:NaOH (pH 7.5), and 200 mM MgCl2 (MCSG2 #H8). To determine the structure of the NADPH/DPA-bound complex, 10 mM NADPH and 10 mM DPA were added to PaDHDPR protein solution for co-crystallization. Crystals of NADPH/DPA-bound PaDHDPR were obtained under conditions of 0.2 M ammonium sulphate, 0.1 M Tris-HCl (pH 8.5), and 25% (w/v) PEG 3350 (MCSG1 #G8). Crystals were directly harvested from the initial screening plate and transferred to cryoprotectant Paratone-N oil (Hampton Research, Aliso Viejo, CA, USA) and flash-frozen with a liquid-nitrogen gas stream. X-ray diffraction data were collected using a BL-5C beam line at the Pohang Accelerator Laboratory (Pohang, Korea). X-ray diffraction data for SeMet-labelled PaDHDPR included 360 images collected at a wavelength of 0.9790 Å. Data for native, DPA-bound, and NADPH/DPA-bound PaDHDPR included 120, 360, and 360 images, respectively. Images were collected at 1° oscillation with an exposure time of 1 s per image. Diffraction data were indexed, processed, and scaled using the HKL-2000 program43. X-ray diffraction data statistics are presented in Table 1.
Structure determination and refinement
The PaDHDPR structure was solved by SAD38. The 2.0-Å data of Se-Met-labelled PaDHDPR was collected at the energy of the Se edge (12.6645 keV) obtained from X-ray energy scans. The AutoSol program from PHENIX generated a partial structure model with Rwork and Rfree values of 27.7% and 29.9%, respectively44,45. The structure of native PaDHDPR was solved by molecular replacement with MOLREP from the CCP4 suite using the partial model of SeMet-labelled PaDHDPR46,47. The model was improved through iterative cycles of manual model building with Coot and refinement with REFMAC in the CCP4 suite48,49. The final model had Rwork and Rfree values of 16.9% and 20.7%, respectively. Molecular replacement for DPA- and NADPH/DPA-bound PaDHDPR was performed using apo PaDHDPR and its individual domains. Rebuilding and refinement were performed in the same manner as for apo PaDHDPR. The final model of DPA-bound PaDHDPR had Rwork and Rfree values of 16.0% and 19.5%, respectively, whereas the final model of NADPH/DPA-bound PaDHDPR had Rwork and Rfree values of 18.7% and 25.7%, respectively. The quality of the final structure models was verified using MolProbity50. Detailed refinement statistics are listed in Table 1. Structure factors and coordinates of apo and DPA- and NADPH/DPA-bound PaDHDPR have been deposited in the PDB with codes 5Z2D, 5Z2E, and 5Z2F, respectively.
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Acknowledgements
We thank the staff at the X-ray core facility of Korea Basic Science Institute (Ochang, Korea) and BL-5C of the Pohang Accelerator Laboratory (Pohang, Korea) for their assistance with data collection. This work was supported by the Korea Polar Research Institute (grant nos. PE16390 and PE18210 to JHL and PE18080 to HP). PDB numbers for the three structures are: 5Z2D (apo PaDHDPR), 5Z2E (DPA-bound PaDHDPR), and 5Z2F (NADPH/DPA-bound PaDHDPR).
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H.P., and J.H.L. designed and supervised the project. C.W.L., S.-H.P., and S.G.L. performed cloning, expression, and protein purification. C.W.L., S.-H.P., and H.J.P. crystallized, collected X-ray data, and solved the protein structures. S.-H.P. and H.H.P. performed the enzyme-activity experiments. S.-H.P. and H.J.K. performed the AUC and ITC assays. C.W.L., S.-H.P., and J.H.L. wrote the initial manuscript, and H.J.P. and H.P. contributed to revisions. All authors discussed the results, commented on the manuscript, and approved the final version.
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Lee, C.W., Park, SH., Lee, S.G. et al. Crystal structure of dihydrodipicolinate reductase (PaDHDPR) from Paenisporosarcina sp. TG-14: structural basis for NADPH preference as a cofactor. Sci Rep 8, 7936 (2018). https://doi.org/10.1038/s41598-018-26291-x
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DOI: https://doi.org/10.1038/s41598-018-26291-x
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