Abstract
ALDH1L1 (10-formyltetrahydrofolate dehydrogenase), an enzyme of folate metabolism highly expressed in liver, metabolizes 10-formyltetrahydrofolate to produce tetrahydrofolate (THF). This reaction might have a regulatory function towards reduced folate pools, de novo purine biosynthesis, and the flux of folate-bound methyl groups. To understand the role of the enzyme in cellular metabolism, Aldh1l1−/− mice were generated using an ES cell clone (C57BL/6N background) from KOMP repository. Though Aldh1l1−/− mice were viable and did not have an apparent phenotype, metabolomic analysis indicated that they had metabolic signs of folate deficiency. Specifically, the intermediate of the histidine degradation pathway and a marker of folate deficiency, formiminoglutamate, was increased more than 15-fold in livers of Aldh1l1−/− mice. At the same time, blood folate levels were not changed and the total folate pool in the liver was decreased by only 20%. A two-fold decrease in glycine and a strong drop in glycine conjugates, a likely result of glycine shortage, were also observed in Aldh1l1−/− mice. Our study indicates that in the absence of ALDH1L1 enzyme, 10-formyl-THF cannot be efficiently metabolized in the liver. This leads to the decrease in THF causing reduced generation of glycine from serine and impaired histidine degradation, two pathways strictly dependent on THF.
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Introduction
Folate coenzymes participate in numerous biochemical reactions of one-carbon transfer1. The network of folate utilizing reactions is referred to as one-carbon metabolism with more than two dozen enzymes participating in folate conversions. These reactions are involved in metabolic pathways of amino acid biosynthesis and degradation, de novo nucleotide biosynthesis, formate clearance, and the regulation of protein biosynthesis in mitochondria1. The role of folate pathways in energy balance in the cell was also underscored2. Higher animals including humans cannot synthesize folate and instead must obtain it from their diet. Folate is indispensable for normal cell function and insufficient dietary folate intake, as well as deregulation of folate metabolism, is associated with several diseases most notably neural tube defects3. Studies of mice with knockouts of folate-related genes have shown that the loss of certain key folate-metabolizing enzymes is embryonically lethal, the effect associated with the crucial role of folate pathways for nucleic acid biosynthesis and methylation processes4,5,6,7,8,9,10.
One of the folate-metabolizing enzymes, ALDH1L1 (10-formyltetrahydrofolate dehydrogenase) is a major cytosolic protein in the liver but its precise biological significance is not clear11. The enzyme converts 10-formyl-THF to THF and CO2 in an NADP+-dependent reaction. This reaction could be important for replenishing the cellular THF pool, which is involved in several metabolic processes in the cell including serine to glycine conversion, histidine degradation, and formate oxidation11,12. ALDH1L1 could also regulate purine levels by competing for 10-formyl-THF, which is a substrate for two reactions in the de novo purine pathway. Furthermore, the enzyme clears one-carbon groups, in the form of CO2, from the folate pool which might limit the overall biosynthetic capacity of folate-dependent reactions thus playing a regulatory role11,13. In support of such proliferation regulatory role, we have recently shown that ALDH1L1 is down-regulated in S-phase of the cell cycle in NIH 3T3 cells14. Several studies also implicated ALDH1L1 as a folate depot, the function likely important for preventing folate degradation15,16,17. Finally, the enzyme can be important as a source of NADPH, the role highlighted for the mitochondrial 10-formyltetrahydrofolate dehydrogenase isozyme2.
Of note, ALDH1L1 belongs to the family of aldehyde dehydrogenases (ALDH)18. The C-terminal domain of the protein has sequence homology with several members of the ALDH family, has a typical ALDH fold and catalyzes in vitro aldehyde dehydrogenase reaction using short-chain aldehydes as substrate11. Though natural aldehyde substrates of ALDH1L1 are not known, such function of the enzyme cannot be excluded at present.
Thus, while the biochemical reactions catalyzed by ALDH1L1 are well characterized, the effect of the enzyme on overall cellular metabolism was not addressed. In support of this notion, a recent study has also implicated ALDH1L1 in the conversion of dihydrofolate to folic acid through an unclear mechanism17. Importantly, the enzyme is strongly down-regulated in cancer cell lines and malignant tumors19,20 through the promoter methylation21 but its role in tumorigenesis and tumor development is not fully understood (reviewed in12,22). In the present study, we have generated Aldh1l1 knockout mice and characterized their reproductive ability, phenotype and the effect of the gene loss on the liver metabolic profile, reduced folate pools and expression of inflammation-related genes. Our study provides experimental evidence that ALDH1L1 regulates reduced folate pools as well as glycine metabolism in the liver.
Results
Generation and characterization of Aldh1l1 −/− mice
We have generated Aldh1l1−/− mice using ES cells obtained from the KOMP consortium. These cells have a “knockout first” Aldh1l1 gene alteration generated via homologous recombination with the gene-trapping vector depicted in Fig. 1a. The trapping cassette was inserted in the intron upstream of exon 3 creating a constitutive null mutation. PCR-based genotyping of the wild type Aldh1l1 allele generated a 199 bp fragment, whereas amplification of the disrupted allele generated a 685 bp fragment (Fig. 1b). The successful knockout of the Aldh1l1 gene was demonstrated by the loss of the ALDH1L1 protein assessed by Western blot assays (Fig. 1c; full-size images are shown in Supplementary Fig. S1). Of note, heterozygous Aldh1l1+/− mice have shown partial decrease in the Aldh1l1 protein level in the liver, suggesting a gene-dosage effect of the protein expression (Supplementary Fig. S2). Surprisingly, the band corresponding to ALDH1L1 was still seen in the pancreas of Aldh1l1−/− mice though it had lower intensity (Fig. 1c). We tested whether this was caused by the cross-reactivity of our polyclonal antibody with mitochondrial isozyme ALDH1L223, which sequence is 75.5% identical to the sequence of ALDH1L1. Of note, pancreas has the highest level of ALDH1L2 among all organs23. This was found to be the case: Western blot assays of cytosolic and mitochondrial fractions of pancreatic tissues have shown the absence of ALDH1L1 in the cytosol (Fig. 1d). We have also evaluated levels of a panel of enzymes relevant to metabolism of 10-formyl-THF (Fig. 1c,e; GNMT was included in the panel because it is one of the most abundant folate-relevant protein in the liver and it regulates the flux of one-carbon groups from folate pools towards methylation or nucleotide biosynthesis24; full-size images are shown in Supplementary Fig. S1). We did not observe a major effect of the Aldh1l1 KO on the levels of tested proteins in three examined organs, liver, pancreas and brain (Fig. 1c).
Both female and male Aldh1l1−/− mice were viable, fertile and developed normally with the total body weight similar to the wild type littermates at weaning (Fig. 1f) and at the age of 6 mo (Supplementary Fig. S3). We observed similar numbers of offspring from mating male or female Aldh1l1−/− with Aldh1l1+/+ mice, as well as from mating mice with both sexes having either Aldh1l1−/− or Aldh1l1+/+ genotype (Fig. 1f). Analysis of litter size from the intercross of Aldh1l1+/− mice revealed that there was no significant variation from the Mendelian distribution ratio for male pups but was some deviation for female pups (Fig. 1g). Breeding experiments with a combination of genotypes (Fig. 1f), however, suggests that such deviation could be the result of an insufficient number of analyzed litters. We identified no phenotypic differences between Aldh1l1−/− and Aldh1l1+/+ mice in terms of growth, body weight and food consumption, and knockout mice appeared healthy. Gross examination of the brain, lungs, kidney, liver, pancreas, heart and spleen did not reveal any noticeable differences in the organ size or morphology between genotypes (Supplementary Fig. S3). H&E staining and histological analysis of the tissues has further indicated the lack of differences between the two genotypes (Fig. 1h). Overall, our study demonstrated that phenotypically, the Aldh1l1−/− genotype is undistinguishable from the Aldh1l1+/+ genotype.
Levels of reduced folate in Aldh1l1 KO mice
We have further evaluated levels of reduced folate pools in livers of Aldh1l1−/− and Aldh1l1+/+ mice. Our method allows the measurement of 10-formyl-THF, 5-methyl-THF, the combination of THF/5,10-methylene-THF, and the combination of dihydrofolate and folic acid25,26. In the liver of both male and female mice we have observed strong differences between the genotypes in two folate pools, 10-formyl-THF and THF/5,10-methylene-THF (Fig. 2). Other pools were not affected in the Aldh1l1−/− genotype when compared to the Aldh1l1+/+ genotype (Fig. 2). These changes are in agreement with the role of ALDH1L1-catalyzed reaction (Fig. 1e). A statistically significant decrease in the total folate (1.2-fold for males and 1.4-fold for females) in the liver was also detected (Fig. 2), which could be associated with the proposed role for the ALDH1L1 protein as folate depot; in such a role, ALDH1L1 would protect reduced folate from degradation. Similar changes were observed in the brain of both sexes though the 10-formyl-THF pool in the brain was not increased as dramatically as in the liver (Fig. 2). Similar to the liver, the total folate was lower in the brain of knockout mice (though such difference for females was not statistically significant, Fig. 2). Of note, no changes in levels of folate in the pancreas or whole blood of male mice were observed between Aldh1l1−/− and Aldh1l1+/+ genotypes (Fig. 2).
Aldh1l1 −/− mice displayed metabolic symptoms of folate deficiency and have altered glycine metabolism
We evaluated the effect of the Aldh1l1 gene loss on the overall metabolic profile in the liver, brain, lung and pancreas of male mice (Supplementary Data Files 1 and 2). We have performed targeted metabolomics analysis to compare changes in common metabolites between Aldh1l1−/− and Aldh1l1+/+ male mice (the summary of the analysis is shown in Supplementary Table S1). We have also performed metabolomic analysis in the livers of female mice. Our analysis included 334 named metabolites detected in liver, 237 in brain, 357 in lung and 318 in pancreatic tissue. OPLS-DA models with good statistics were generated for all tissues (Fig. 3a,b and Supplementary Fig. S4). However, top 25 metabolites discriminating Aldh1l1−/− and Aldh1l1+/+ male mice were different between liver and other tissues (Supplementary Table S2; three metabolites out of 25 were common between liver and lung and one metabolite between liver and brain or pancreas). We focused further analysis on the liver metabolome (OPLS-DA for liver shows good segregation between Aldh1l1−/− and Aldh1l1+/+ genotypes for both male and female mice, Fig. 3b). This analysis indicated that KO mice experience functional folate deficiency, the conclusion based on the accumulation of formiminoglutamate (FIGLU), which was increased about 11-fold in livers of KO mice (Fig. 3c). FIGLU is the intermediate in the folate-dependent histidine degradation pathway27 and a marker of folate deficiency28. Dihydrofolate was also strongly depleted (about 3.5-fold) in these animals (Fig. 3c), which is in agreement with folate deficiency and with the overall decrease in reduced folate pools identified by the ternary complex assay (Fig. 2). These data also indicated alterations in glycine metabolism (the drop of glycine as well as glycine conjugates with fatty acids, Fig. 3c). Of note, similar changes in these metabolites were observed in livers of both male and female mice (Fig. 3c).
To confirm our findings, the second metabolomics analysis of liver tissues from male mice was performed using higher sample numbers (5 samples per group, Supplementary Data File 3). This analysis has extended the number of named metabolites to 628 with the difference for 91 metabolites being statistically significant between the two genotypes (31 compounds being decreased and 60 being elevated in Aldh1l1−/− compared to the Aldh1l1+/+ mice, Fig. 4a). PCA has shown good segregation between the two genotypes (Supplementary Fig. S5) and the OPLS-DA model had good statistics (Fig. 4b). This analysis confirmed findings from the previous experiment that KO mice show metabolic signs of functional folate deficiency. Specifically, FIGLU was increased more than 15-fold in KO mice (Fig. 4c). Furthermore, tissue folic acid and dihydrofolate were strongly depleted (more than 2-fold and 5-fold, respectively) in these animals, which would be in agreement with folate deficiency (Fig. 4c).
Similar to the first metabolomic experiment, a 2-fold statistically significant decrease in glycine was detected in Aldh1l1−/− compared to the Aldh1l1+/+ mice (Fig. 4c). Strong drop in numerous glycine conjugates was also observed in the KO genotype (Fig. 4c and Supplementary Table S2), which was interpreted as the overall shortage of glycine in Aldh1l1−/− mice. In fact, the top nine compounds differentiating Aldh1l1−/− mice from Aldh1l1+/+ mice were glycine, glycine conjugates and two folate metabolites, with four of those compounds also identified in our first metabolomic analysis (Fig. 4c). Additional glycine conjugates were also decreased in livers of knockout mice with VIP values indicating their strong contribution to the metabolic differentiation of the two genotypes (Fig. 4c). The depletion of glycine was likely caused by the decrease of the THF-dependent synthesis of glycine from serine (schematically depicted in Fig. 4d), with the underlying basis for this phenomenon being the decrease of the THF pool observed in Aldh1l1−/− mice (Fig. 2). In agreement with this mechanism, we also observed statistically significant elevation of serine in livers of the KO mice (fold-change was 1.38, p = 0.872, VIP was 1.14). Our data indicate the direct effect of the ALDH1L1 loss on glycine metabolism in liver.
Effects of the ALDH1L1 loss on the gene expression profile
Since ALDH1L1 protein has been established as a regulator of proliferation12,19, its loss is expected to evoke a cellular response with regard to altered gene expression. The diminished antioxidant pool in livers of Aldh1l1−/− mice (Supplementary Data File 3) raised the question of whether the loss of ALDH1L1 specifically affected the expression of genes associated with inflammation. In fact, ALDH1L1 is one of the most strongly downregulated genes in hepatocellular carcinoma in humans20, a typical inflammation-related cancer29. To investigate the effect of Aldh1l1 knockout on gene expression profile, we performed customized NanoString analysis of mRNAs in 3-months-old male mice (Supplementary Data File 4). This panel includes a total of 242 genes relevant to inflammation and the immune response. PCA has shown that the two genotypes are well separated (Fig. 5a). The heat map (Fig. 5b) illustrates the strong difference between wild type and KO mice with regard to the expression level of the proteins included in the panel. Overall, expression levels of numerous tested targets were higher in the KO mice (Fig. 5b). A univariate analysis of targets based on the fold-change >1.5 and p-value < 0.05 cut-offs allowed the identification of a group of 25 genes most differentially expressed between the two groups (Fig. 5c). To further identify transcripts that contribute most to the discrimination between the wild type and knock out mice, we have performed a multivariate analysis applying two different supervised machine learning methods to the standardized data. Unlike the unsupervised PCA, these two methods, Linear Discriminant Analysis (LDA) and Partial Least Squares-Discriminant Analysis (PLS-DA), take advantage of the known sample type information and can quantify the relative contribution of each protein to the separation of the wild type and knockout mice30,31. LDA uses the coefficients/weights and PLS-DA uses the VIP values, respectively, for this purpose. Figure 5d shows the top 20 discriminating proteins based on LDA and PLS-DA analysis. Cross-reference of the genes identified by different approaches has defined a subset of genes differentially expressed at the mRNA level in Aldh1l1−/− compared to Aldh1l1+/+ mice (Fig. 5d). A significant portion of these genes overlaps with the top 25 targets identified by the univariate analysis. Among these genes were growth factors, transcription factors, and inflammatory and immune response markers. Overall, these data suggest that ALDH1L1 protein expression is important for the control of inflammation in the liver.
Discussion
ALDH1L1 is an enzyme of folate metabolism with a putative regulatory function. The enzyme is present at high levels in the liver, the main organ of folate metabolism, where it constitutes about 1% of the total liver cytosolic protein32. High expression levels of ALDH1L1 protein were also detected in kidney and pancreas, suggesting a function for the enzyme in these organs19,23. Several studies have provided evidence that ALDH1L1 could be important in neural system function or development as well. Thus, an early report has demonstrated that the expression of the protein is regulated in the cerebellum of mice during development33. Further study has confirmed the specific midline expression of ALDH1L1 and provided evidence that this enzyme has restricted expression in the developing neural tube34. In fact, this study demonstrated that ALDH1L1 upregulation during central nervous system development correlates with reduced proliferation and most midline ALDH1L1-positive cells are quiescent34. Such expression pattern correlates with the antiproliferative effect of the enzyme observed in a cell culture system19. Curiously, ALDH1L1 was identified as a pan-astrocyte marker35. ALDH1L1 is also expressed in neurons and oligodendrocytes, and its expression is decreased in the spinal cord during postnatal maturation but is up-regulated in reactive astrocytes after neural injury or chronic neurodegeneration36. Therefore, the loss of ALDH1L1 could be expected to have an effect on mouse phenotype.
A report of mice lacking ALDH1L1 as the result of irradiation with fission-spectrum neutrons (NEUT2 mice) indicated that the Aldh1l1 gene is not essential for viability in the mouse37. Aldh1l1−/− mice generated in our study and NEUT2 mice have shown similar alterations in hepatic reduced folate pools with a strong accumulation of 10-formyl-THF and a significant drop in THF levels, changes consistent with the loss of the 10-formyl-THF dehydrogenase activity. In addition, the total hepatic folate was also reduced upon ALDH1L1 loss, which could be a result of decreased protection of folate coenzymes from degradation15. In the cell, cytosolic 10-formyl-THF can be also metabolized through two other pathways1: (i) two reactions of the de novo purine biosynthesis; and (ii) the conversion to 5,10-methylene-THF by a trifunctional enzyme MTHFD1. The latter reaction though is reversible and at normal conditions it proceeds towards 10-formyl-THF generation. Of note, MTHFD1 also generates 10-formyl-THF from THF and formate in the ATP-dependent synthetase reaction (Fig. 1e)1. However, our study indicates that these pathways cannot compensate for the loss of ALDH1L1 with regard to 10-formyl-THF utilization. Of note, levels of corresponding enzymes were not changed upon the ALDH1L1 loss (Fig. 1c).
In contrast to mice generated in the present study, the chromosomal deletion in NEUT2 mice has also caused the loss of additional 27 genes, including urocanase 1, the gene involved in the histidine degradation pathway34. This pathway donates one-carbon groups to the folate pool and thus requires THF27. It has been suggested that, by decreasing the overall use of THF, the deletion of the urocanase 1 gene partially compensates for the decrease of the THF pool caused by the ALDH1L1 loss, that prevents the genotype manifestation34,38. This might explain the lack of a severe effect of the Aldh1l1 loss in these mice. In our study, however, Aldh1l1−/− mice also did not show noticeable phenotypic changes compared to Aldh1l1+/+ mice. Yet, metabolomic analysis did show the accumulation of FIGLU, an intermediate of the folate-dependent histidine degradation, in livers of Aldh1l1−/− mice. This finding suggested the impairment of this pathway in Aldh1l1 knockout mice, that is in agreement with the low THF levels (THF is required to metabolize FIGLU, Fig. 4d). Of note, elevated FIGLU in urine is used as a marker for folate deficiency28. Thus, the Aldh1l1 loss is associated with functional folate deficiency in mouse liver even though the intake of dietary folate was not limited. The blood folate levels were also not changed compared to wild-type mice. Our study indicates that in the absence of ALDH1L1 enzyme, 10-formyl-THF cannot be metabolized and accumulates creating a folate trap, the situation similar to the methyl-folate trap39. Consistently, the drop in THF levels could be expected to cause decreased generation of glycine from serine as well as impaired histidine degradation, two pathways strictly dependent on THF.
In our study, the loss of ALDH1L1 also had a strong effect on glycine levels as well as levels of several glycine conjugates, which were dramatically reduced in knockout animals (Figs. 3c and 4c). We interpret the decrease of the glycine conjugates as the consequence of the overall shortage of glycine. Humans obtain only about 20% of glycine from their diet while about 80% is synthesized in the body40. In higher animals, four biosynthetic pathways can produce glycine, with two of these pathways being folate-dependent41,42. The pathway from glucose to glycine includes, as the final step, the THF-dependent conversion of serine to glycine. The pathway from betaine includes two THF-dependent reactions (Fig. 4d), the conversion of (i) dimethylglycine to sarcosine and (ii) sarcosine to glycine. While all three folate-dependent reactions require THF as the acceptor of one-carbon groups, the effect of ALDH1L1 on the betaine pathway is less apparent since both of its folate-dependent reactions reside in the mitochondrion1. Glycine, a common non-essential amino acid, in addition to its role in protein biosynthesis is the participant of numerous metabolic pathways including purine, creatine, heme and glutathione biosynthesis41. Thus, the depletion of glycine could be the cause of several secondary effects on metabolism such as the decrease in glutathione, lower availability of ascorbate, impaired mitochondrial lipid metabolism, oxidative stress, and less efficient remediation of toxic products43,44,45,46,47,48. Glycine is also a donor of one-carbon groups to the mitochondrial folate metabolism, through the glycine cleavage system49,50. Since the ALDH1L1 loss decreases both glycine and THF pools, the impairment of mitochondrial folate metabolism, due to decreased glycine cleavage, could be expected. This, however, needs to be tested experimentally.
The accumulation of 10-formyl-THF in Aldh1l1−/− mice did not lead to the increase of levels of purine nucleotides. However, the effect of ALDH1L1 on purine biosynthesis would be crucial for rapidly proliferating cells but not quiescent cells. This is in agreement with the phenomenon that the ALDH1L1 expression is down-regulated in S-phase of the cell cycle but not in quiescent cells14. In the liver, overwhelming majority of cells are in non-proliferating state51, which would explain the lack of the effect of the Aldh1l1 KO on purine pools, and this is also in agreement with the 10-formyl-THF accumulation (otherwise 10-formyl-THF would be taken up by the purine biosynthesis). Taking into account the decrease in glycine, a substrate for purine biosynthesis, in the liver of Aldh1l1−/− mice, the effect of ALDH1L1 on the de novo purine pathway is more complex and to address this question with more certainty tracer experiments should be pursued. Curiously, the NanoString array has shown a significant elevation of the ATF4 transcription factor in Aldh1l1-/- mice. ATF4 is involved in the regulation of serine and glycine biosynthesis and one-carbon metabolism52,53,54,55,56, as well as biosynthesis of purines through the control of the mitochondrial folate metabolism57. This raises the question of whether the ATF4 up-regulation in Aldh1l1 KO mice is a compensatory mechanism in response to glycine decrease or in response to disbalance of folate pools.
Loss of glycine conjugates in Aldh1l1−/− mice can have a diverse downstream effect on the cell. Conjugation of glycine with carboxylic acids, for example, is an important detoxification process48. Glycine conjugation with mitochondrial acyl-CoAs, catalyzed by glycine N-acyltransferase, is also an essential metabolic pathway responsible for maintaining adequate levels of free coenzyme A58. Acylglycine conjugates by themselves can also have important functions in the cell. Thus, N-acyl glycines are structurally related to endocannabinoids and at least one of such conjugates, N-arachidonoyl glycine, is a potent inhibitor of the enzyme primarily responsible for the degradation of the endocannabinoid anandamide59. Furthermore, several of glycine conjugates have signaling functions in the cell59. Of note, glycine itself is a neurotransmitter and its decrease can have a far-reaching effects beyond metabolic alterations60.
Glycine is an effective cytoprotective agent. In experimental models, glycine has a protective effect against a variety of diseases including ischemia/reperfusion injury, shock, transplantation, alcoholic hepatitis, hepatic fibrosis, arthritis, and tumor and drug toxicity (reviewed in47). Such protective function could be a direct effect on target cells or could be mediated by inhibition of inflammatory cell activation. In fact, there is also a growing body of evidence that glycine functions as an anti-inflammatory and immunomodulatory agent, and it has been used to modulate acute systemic inflammatory responses after powerful external stimuli such as multiple trauma or sepsis42. In this regard, Aldh1l1−/− mice demonstrated noticeable changes in levels of proteins involved in immune response and inflammation, a likely response to the shift in glycine metabolism. Of note, folate supplementation is associated with reduced inflammation at certain physiological conditions while folate depletion led to high expression of pro-inflammatory mediators in cultured macrophages (reviewed in61). The link between folate and inflammatory response is likely to play a role in NAFLD which also implicates ALDH1L1 as a part of this mechanism. Indeed, our data indicate a metabolically compromised liver function in Aldh1l1−/− mice though it does not manifest as an apparent phenotype.
Interestingly, it has been reported that the knockout of another folate enzyme, cytosolic serine hydroxymethyltransferase (SHMT1), also did not cause an obvious phenotype62. In this regard, ALDH1L1 and SHMT1 catalyze two consecutive steps in the glycine pathway (Fig. 4d). In contrast to Aldh1l1−/− mice, glycine levels were not changed in Shmt1−/− mice62. One explanation of this phenomenon could be the alternative splicing of the Shmt2 gene, which encodes mitochondrial serine hydroxymethyltransferase. The omission, during Shmt2 gene splicing, of the exon, coding for the mitochondrial leader sequence, produces cytoplasmic isozyme of SHMT63. This mechanism may also account for the viability of Shmt1−/− mice63. Such mechanism could be envisioned for Aldh1l1−/− mice as well since the gene encoding mitochondrial 10-formyltetrahydrofolate dehydrogenase, Aldh1l2, is present in genomes of higher animals including mice and humans. The alternative splicing of the Aldh1l2 gene, which would produce a cytosolic enzyme, is not known and our data indicate that in Aldh1l1−/− mice there is no cytosolic 10-formyltetrahydrofolate dehydrogenase. Observed changes in THF and 10-formyl-THF pools in the liver are in agreement with this conclusion.
In summary, our study of Aldh1l1 KO mice has shown that cytosolic 10-formyl-THF dehydrogenase is a key enzyme to supply THF for glycine biosynthesis in the liver and thus is an important component of the glycine metabolic network. These findings provide a mechanistic basis for the association between ALDH1L1 gene and serine to glycine ratio in serum64. An open question remains whether dietary folate deficiency can exacerbate the metabolic effects of Aldh1l1−/− thus causing pathologies as it has been demonstrated for Shmt1 KO mice65.
Methods
Generation of Aldh1l1 knockout mice
All animal experiments were conducted in strict accordance with the National Institutes of Health’s “Guide for Care and Use of Laboratory Animals” and were approved by the Institutional Animal Care and Use Committee at the Medical University of South Carolina (MUSC), Charleston, South Carolina and by the Institutional Animal Care and Use Committee at the David H. Murdock Research Institute (DHMRI), Kannapolis, North Carolina. Mice were housed in microisolator cages on a 12-h light/dark cycle and allowed access to water and chow ad libitum.
ES cells (clone EPD0132_6_G09, vial #288863; Aldh1l1 targeted, KO first) were obtained from the NCRR-NIH supported KOMP Repository (www.komp.org). This clone was from the C57BL/6N background with the agouti gene engineered into the agouti locus. Mice were generated at the MUSC Transgenic and Genome Editing Core Facility. ES cells (passage 4) have been expanded and injected into freshly isolated C57BL/6-TyrC blastocysts (10 ES cells per blastocyte), which were further injected into CD-1 pseudo-pregnant mice (12 blastocysts per mouse). Twelve chimeric pups were born from three injected females and at weaning we obtained five male chimeric mice, four having 85–99% and one 40% of the black coat color. All male chimera were bred to C57BL/6-TyrC females and black pups (both male and female) were tested for germline transmission of the targeted gene. All of the chimeras showed germline transmission. Heterozygotes for the targeted allele were bred back to the C57BL/6N mice. Heterozygous (Aldh1l1+/−) males and females were bred to obtain knockout and wild type littermates for the study.
Genotyping
Genotyping was carried out by polymerase chain reaction (PCR) of tail lysates obtained using direct PCR (Tail) lysis reagent (cat #101-T) and Proteinase K (Specific activity >600 U/ml, Thermo Scientific, cat #EO0491). Primers for genotyping are shown in Supplementary Table S3. Amplification generates 199 bp amplicon for the WT allele and 685 bp amplicon for the mutant allele. Heterozygous males and females were intercrossed to obtain knockout and wildtype littermates.
Western blot assays
Total protein was prepared from flash frozen tissues. Approximately 300 mg tissue was minced and homogenized in 1 ml of RIPA or IP-lysis buffer (Thermo Scientific, Pittsburg, PA) with protease and phosphatase inhibitors (1:100, Sigma-Aldrich, St. Louis, MO). Proteins were resolved by SDS-PAGE in 4–15% gradient gels (Novex, Invitrogen, Carlsbad, CA) and transferred to PVDF membranes (Millipore, Bedford, MA) in transfer buffer containing 10% methanol. Membranes were probed with primary antibodies in Tris-buffered saline with Tween- 20 containing 5% nonfat milk or BSA. A detailed description of the primary antibodies is provided in Supplementary Table S4. Horseradish peroxidase–conjugated secondary antibodies (GE Healthcare) were used at 1:5000 dilution and signal assessed with Super Signal West Pico chemiluminescence substrate (ThermoFisher Scientific, Waltham, MA, USA).
Preparation of mitochondrial and cytosolic fractions
Mitochondria were isolated by differential centrifugation using the Mitochondria Isolation kit (Abcam, Cambridge, UK) according to the manufacture’s protocol and were solubilized using IP-lysis buffer. Following the isolation of mitochondria, the supernatant representing the cytosol was spun at 22,000 × g for 30 min and the resultant supernatant was concentrated five-fold in a centrifugal concentrator with a molecular weight cut-off of 10,000 (Merck-Millipore, Burlington, MA).
Assays of reduced folate pools
Samples were prepared essentially as we described66. Fifty mg of tissues were homogenized on ice in 1 ml of 50 mM Tris-HCl buffer, pH 7.4 containing 50 mM sodium ascorbate using a Dounce homogenizer and lysed by heating for 3 min in a boiling water bath. In the case of whole blood, 50 μl of sample were mixed with 450 μl of the same buffer and treated as above. Lysates were chilled on ice and centrifuged for 5 min at 17,000 × g at 4 °C. Folate pools were measured in tissue lysates by the ternary complex assay method as we described previously25,26. Folate levels were calculated per milligram of protein measured by Bradford assay or per milliliter of whole blood. Statistical analysis was carried out using GraphPad Prism VIII software. Statistical significance was calculated using Student’s t-test.
Metabolomic analysis
Metabolomics was performed using commercial services from Metabolon (Durham, NC). Individual samples (100–200 mg of flash frozen tissue) were subjected to methanol extraction then split into aliquots for analysis by ultrahigh performance liquid chromatography/mass spectrometry (UHPLC/MS). The global biochemical profiling analysis comprised of four unique arms consisting of reverse phase chromatography positive ionization methods optimized for hydrophilic compounds (LC/MS Pos Polar) and hydrophobic compounds (LC/MS Pos Lipid), reverse phase chromatography with negative ionization conditions (LC/MS Neg), as well as a HILIC chromatography method coupled to negative (LC/MS Polar)67. All of the methods alternated between full scan MS and data dependent MSn scans. The scan range varied slightly between methods but generally covered 70–1000 m/z. Metabolites were identified by automated comparison of the ion features in the experimental samples to a reference library of chemical standard entries that included retention time, molecular weight (m/z), preferred adducts, and in-source fragments as well as associated MS spectra and curated by visual inspection for quality control using software developed at Metabolon. Identification of known chemical entities was based on comparison to metabolomic library entries of purified standards68.
Statistical analysis
Analysis was carried out essentially as described69,70,71,72. Two types of statistical analyses were performed: (1) significance tests and (2) classification analysis. Standard statistical analyses were performed in Array Studio software package on log‐transformed data. For analyses not standard in Array Studio, the R program (http://cran.r-project.org/) was used. Following log transformation and imputation of missing values, if any, with the minimum observed value for each compound, Welch’s two-sample t-test was used as significance test to identify biochemicals that differed significantly (p < 0.05) between experimental groups. An estimate of the false discovery rate (q‐value) was calculated to take into account the multiple comparisons that normally occur in metabolomic‐based studies. Classification analyses used included principal components analysis (PCA), hierarchical clustering, and OPLS-DA. For the scaled intensity graphics, each biochemical in the original scale (raw area count) was rescaled to set the median across all samples equal to 1.
NanoString
RNA was extracted using a mRNeasy micro kit (Qiagen, Valencia, CA, Catalog #217084), and the gene expression analysis was performed by the MUSC deep sequencing and microarray core facility using a custom array and nCounter multiplex analysis (NanoString nCounter Technologies, Inc., Seattle, WA). Raw intensity values (counts) obtained from the analysis were normalized using counts for six housekeeping genes included in the array.
Histological examination
Three-month-old mice were euthanized using CO2, organs were harvested, fixed using 4% paraformaldehyde in sodium phosphate buffer pH 7.4, and embedded in paraffin. Five-micrometer sections were stained with H&E. Examined organs were liver, spleen, kidney, heart and lungs.
Data availability
All data generated or analyzed during this study are included in this published article or its supplementary information files.
References
Tibbetts, A. S. & Appling, D. R. Compartmentalization of Mammalian folate-mediated one-carbon metabolism. Annu Rev Nutr 30, 57–81, https://doi.org/10.1146/annurev.nutr.012809.104810 (2010).
Fan, J. et al. Quantitative flux analysis reveals folate-dependent NADPH production. Nature 510, 298–302, https://doi.org/10.1038/nature13236 (2014).
Blom, H. J., Shaw, G. M., den Heijer, M. & Finnell, R. H. Neural tube defects and folate: case far from closed. Nat Rev Neurosci 7, 724–731 (2006).
Momb, J. et al. Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice. Proc Natl Acad Sci USA 110, 549–554, https://doi.org/10.1073/pnas.1211199110 (2013).
Li, Q. et al. Knockout of dihydrofolate reductase in mice induces hypertension and abdominal aortic aneurysm via mitochondrial dysfunction. Redox Biol 24, 101185, https://doi.org/10.1016/j.redox.2019.101185 (2019).
Raz, S., Stark, M. & Assaraf, Y. G. Folylpoly-gamma-glutamate synthetase: A key determinant of folate homeostasis and antifolate resistance in cancer. Drug Resist Updat 28, 43–64, https://doi.org/10.1016/j.drup.2016.06.004 (2016).
MacFarlane, A. J. et al. Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism. J Biol Chem 284, 1533–1539, https://doi.org/10.1074/jbc.M808281200 (2009).
Di Pietro, E., Sirois, J., Tremblay, M. L. & MacKenzie, R. E. Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is essential for embryonic development. Mol Cell Biol 22, 4158–4166 (2002).
Swanson, D. A. et al. Targeted disruption of the methionine synthase gene in mice. Mol Cell Biol 21, 1058–1065, https://doi.org/10.1128/MCB.21.4.1058-1065.2001 (2001).
Tani, H. et al. Mice deficient in the Shmt2 gene have mitochondrial respiration defects and are embryonic lethal. Sci Rep 8, 425, https://doi.org/10.1038/s41598-017-18828-3 (2018).
Krupenko, S. A. FDH: an aldehyde dehydrogenase fusion enzyme in folate metabolism. Chem. Biol. Interact. 178, 84–93 (2009).
Krupenko, S. A. & Krupenko, N. I. Loss of ALDH1L1 folate enzyme confers a selective metabolic advantage for tumor progression. Chem Biol Interact 302, 149–155, https://doi.org/10.1016/j.cbi.2019.02.013 (2019).
Anguera, M. C. et al. Regulation of Folate-mediated One-carbon Metabolism by 10-Formyltetrahydrofolate Dehydrogenase. J Biol Chem 281, 18335–18342 (2006).
Khan, Q. A. et al. CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase. PLoS One 13, e0199699, https://doi.org/10.1371/journal.pone.0199699 (2018).
Chang, W. N. et al. Knocking down 10-Formyltetrahydrofolate dehydrogenase increased oxidative stress and impeded zebrafish embryogenesis by obstructing morphogenetic movement. Biochim Biophys Acta 1840, 2340–2350, https://doi.org/10.1016/j.bbagen.2014.04.009 (2014).
Hsiao, T. H. et al. Ethanol-induced upregulation of 10-formyltetrahydrofolate dehydrogenase helps relieve ethanol-induced oxidative stress. Mol Cell Biol 34, 498–509, https://doi.org/10.1128/MCB.01427-13 (2014).
Zheng, Y. et al. Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells. Cell 175, 1546–1560 e1517, https://doi.org/10.1016/j.cell.2018.09.041 (2018).
Marchitti, S. A., Brocker, C., Stagos, D. & Vasiliou, V. Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily. Expert Opin Drug Metab Toxicol 4, 697–720, https://doi.org/10.1517/17425255.4.6.697 (2008).
Krupenko, S. A. & Oleinik, N. V. 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells. Cell Growth Differ. 13, 227–236 (2002).
Tackels-Horne, D. et al. Identification of differentially expressed genes in hepatocellular carcinoma and metastatic liver tumors by oligonucleotide expression profiling. Cancer 92, 395–405 (2001).
Oleinik, N. V., Krupenko, N. I. & Krupenko, S. A. Epigenetic silencing of ALDH1L1, a metabolic regulator of cellular proliferation, in cancers. Genes and Cancer 2, 130–139 (2011).
Krupenko, S. A. & Krupenko, N. I. ALDH1L1 and ALDH1L2 Folate Regulatory Enzymes in Cancer. Adv Exp Med Biol 1032, 127–143, https://doi.org/10.1007/978-3-319-98788-0_10 (2018).
Krupenko, N. I. et al. ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase. J. Biol. Chem. 285, 23056–23063, https://doi.org/10.1074/jbc.M110.128843 (2010).
DebRoy, S. et al. A novel tumor suppressor function of glycine N-methyltransferase is independent of its catalytic activity but requires nuclear localization. PLoS One 8, e70062, https://doi.org/10.1371/journal.pone.0070062 (2013).
Oleinik, N. V., Krupenko, N. I., Reuland, S. N. & Krupenko, S. A. Leucovorin-induced resistance against FDH growth suppressor effects occurs through DHFR up-regulation. Biochem Pharmacol 72, 256–266 (2006).
Hoeferlin, L. A., Oleinik, N. V., Krupenko, N. I. & Krupenko, S. A. Activation of p21-Dependent G1/G2 Arrest in the Absence of DNA Damage as an Antiapoptotic Response to Metabolic Stress. Genes Cancer 2, 889–899, https://doi.org/10.1177/1947601911432495 (2011).
Shane, B. & Stokstad, E. L. Vitamin B12-folate interrelationships. Annu Rev Nutr 5, 115–141 (1985).
Cooperman, J. M. & Lopez, R. The role of histidine in the anemia of folate deficiency. Exp Biol Med (Maywood) 227, 998–1000 (2002).
Hou, X. J. et al. Immune response involved in liver damage and the activation of hepatic progenitor cells during liver tumorigenesis. Cell Immunol 326, 52–59, https://doi.org/10.1016/j.cellimm.2017.08.004 (2018).
Lee, L. C., Liong, C. Y. & Jemain, A. A. Partial least squares-discriminant analysis (PLS-DA) for classification of high-dimensional (HD) data: a review of contemporary practice strategies and knowledge gaps. Analyst 143, 3526–3539, https://doi.org/10.1039/c8an00599k (2018).
Ye, J. & Li, Q. A two-stage linear discriminant analysis via QR-decomposition. IEEE Trans Pattern Anal Mach Intell 27, 929–941, https://doi.org/10.1109/TPAMI.2005.110 (2005).
Kisliuk, R. L. In Antifolate drugs in cancer therapy (ed. Jackman, A. L.) 13–36 (Humana Press, 1999).
Kuhar, S. G. et al. Changing patterns of gene expression define four stages of cerebellar granule neuron differentiation. Development 117, 97–104 (1993).
Anthony, T. E. & Heintz, N. The folate metabolic enzyme ALDH1L1 is restricted to the midline of the early CNS, suggesting a role in human neural tube defects. J Comp Neurol 500, 368–383 (2007).
Cahoy, J. D. et al. A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci 28, 264–278 (2008).
Garwood, C. J. et al. Review: Astrocytes in Alzheimer’s disease and other age-associated dementias: a supporting player with a central role. Neuropathol Appl Neurobiol 43, 281–298, https://doi.org/10.1111/nan.12338 (2017).
Champion, K. M., Cook, R. J., Tollaksen, S. L. & Giometti, C. S. Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice. Proc Natl Acad Sci USA 91, 11338–11342 (1994).
Cook, R. J. Disruption of histidine catabolism in NEUT2 mice. Arch Biochem Biophys 392, 226–232 (2001).
Elmore, C. L. et al. Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase. Mol Genet Metab 91, 85–97 (2007).
Robert, J. J., Bier, D. M., Zhao, X. H., Matthews, D. E. & Young, V. R. Glucose and insulin effects on the novo amino acid synthesis in young men: studies with stable isotope labeled alanine, glycine, leucine, and lysine. Metabolism 31, 1210–1218 (1982).
Adeva-Andany, M. et al. Insulin resistance and glycine metabolism in humans. Amino Acids 50, 11–27, https://doi.org/10.1007/s00726-017-2508-0 (2018).
Wang, W. et al. Glycine metabolism in animals and humans: implications for nutrition and health. Amino Acids 45, 463–477, https://doi.org/10.1007/s00726-013-1493-1 (2013).
Alves, A., Bassot, A., Bulteau, A. L., Pirola, L. & Morio, B. Glycine Metabolism and Its Alterations in Obesity and Metabolic Diseases. Nutrients 11, https://doi.org/10.3390/nu11061356 (2019).
Heidari, R. et al. Mitochondria protection as a mechanism underlying the hepatoprotective effects of glycine in cholestatic mice. Biomed Pharmacother 97, 1086–1095, https://doi.org/10.1016/j.biopha.2017.10.166 (2018).
Nguyen, D., Samson, S. L., Reddy, V. T., Gonzalez, E. V. & Sekhar, R. V. Impaired mitochondrial fatty acid oxidation and insulin resistance in aging: novel protective role of glutathione. Aging Cell 12, 415–425, https://doi.org/10.1111/acel.12073 (2013).
Linster, C. L. & Van Schaftingen, E. Vitamin C. Biosynthesis, recycling and degradation in mammals. FEBS J 274, 1–22, https://doi.org/10.1111/j.1742-4658.2006.05607.x (2007).
Zhong, Z. et al. L-Glycine: a novel antiinflammatory, immunomodulatory, and cytoprotective agent. Curr Opin Clin Nutr Metab Care 6, 229–240, https://doi.org/10.1097/01.mco.0000058609.19236.a4 (2003).
Waluk, D. P., Schultz, N. & Hunt, M. C. Identification of glycine N-acyltransferase-like 2 (GLYATL2) as a transferase that produces N-acyl glycines in humans. FASEB J 24, 2795–2803, https://doi.org/10.1096/fj.09-148551 (2010).
Narisawa, A. et al. Mutations in genes encoding the glycine cleavage system predispose to neural tube defects in mice and humans. Hum Mol Genet 21, 1496–1503, https://doi.org/10.1093/hmg/ddr585 (2012).
Pai, Y. J. et al. Glycine decarboxylase deficiency causes neural tube defects and features of non-ketotic hyperglycinemia in mice. Nat Commun 6, 6388, https://doi.org/10.1038/ncomms7388 (2015).
Michalopoulos, G. K. Hepatostat: Liver regeneration and normal liver tissue maintenance. Hepatology 65, 1384–1392, https://doi.org/10.1002/hep.28988 (2017).
Ye, J. et al. Pyruvate kinase M2 promotes de novo serine synthesis to sustain mTORC1 activity and cell proliferation. Proc Natl Acad Sci USA 109, 6904–6909, https://doi.org/10.1073/pnas.1204176109 (2012).
Bao, X. R. et al. Mitochondrial dysfunction remodels one-carbon metabolism in human cells. Elife 5, https://doi.org/10.7554/eLife.10575 (2016).
Celardo, I., Lehmann, S., Costa, A. C., Loh, S. H. & Miguel Martins, L. dATF4 regulation of mitochondrial folate-mediated one-carbon metabolism is neuroprotective. Cell Death Differ 24, 638–648, https://doi.org/10.1038/cdd.2016.158 (2017).
Selvarajah, B. et al. mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-beta1-induced collagen biosynthesis. Sci Signal 12, https://doi.org/10.1126/scisignal.aav3048 (2019).
Xia, Y. et al. Metabolic Reprogramming by MYCN Confers Dependence on the Serine-Glycine-One-Carbon Biosynthetic Pathway. Cancer Res 79, 3837–3850, https://doi.org/10.1158/0008-5472.CAN-18-3541 (2019).
Ben-Sahra, I., Hoxhaj, G., Ricoult, S. J., Asara, J. M. & Manning, B. D. mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. Science 351, 728–733, https://doi.org/10.1126/science.aad0489 (2016).
Badenhorst, C. P., van der Sluis, R., Erasmus, E. & van Dijk, A. A. Glycine conjugation: importance in metabolism, the role of glycine N-acyltransferase, and factors that influence interindividual variation. Expert Opin Drug Metab Toxicol 9, 1139–1153, https://doi.org/10.1517/17425255.2013.796929 (2013).
Burstein, S. H. N-Acyl Amino Acids (Elmiric Acids): Endogenous Signaling Molecules with Therapeutic Potential. Mol Pharmacol 93, 228–238, https://doi.org/10.1124/mol.117.110841 (2018).
Bowery, N. G. & Smart, T. G. GABA and glycine as neurotransmitters: a brief history. Br J Pharmacol 147(Suppl 1), S109–119, https://doi.org/10.1038/sj.bjp.0706443 (2006).
Sid, V., Siow, Y. L. & O, K. Role of folate in nonalcoholic fatty liver disease. Can J Physiol Pharmacol 95, 1141–1148, https://doi.org/10.1139/cjpp-2016-0681 (2017).
MacFarlane, A. J. et al. Cytoplasmic serine hydroxymethyltransferase regulates the metabolic partitioning of methylenetetrahydrofolate but is not essential in mice. J Biol Chem 283, 25846–25853, https://doi.org/10.1074/jbc.M802671200 (2008).
Anderson, D. D. & Stover, P. J. SHMT1 and SHMT2 are functionally redundant in nuclear de novo thymidylate biosynthesis. PLoS One 4, e5839, https://doi.org/10.1371/journal.pone.0005839 (2009).
Dharuri, H. et al. Automated workflow-based exploitation of pathway databases provides new insights into genetic associations of metabolite profiles. BMC Genomics 14, 865, https://doi.org/10.1186/1471-2164-14-865 (2013).
Beaudin, A. E. et al. Dietary folate, but not choline, modifies neural tube defect risk in Shmt1 knockout mice. Am J Clin Nutr 95, 109–114, https://doi.org/10.3945/ajcn.111.020305 (2012).
Oleinik, N. V., Helke, K. L., Kistner-Griffin, E., Krupenko, N. I. & Krupenko, S. A. Rho GTPases RhoA and Rac1 mediate effects of dietary folate on metastatic potential of A549 cancer cells through the control of cofilin phosphorylation. J Biol Chem 289, 26383–26394, https://doi.org/10.1074/jbc.M114.569657 (2014).
Evans, A. M. et al. High resolution mass spectrometry improves data quantity and quality as compared to unit mass resolution mass spectrometry in highthroughput profiling metabolomics. Matabolomics 4, https://doi.org/10.4172/2153-0769.1000132 (2014).
Dehaven, C. D., Evans, A. M., Dai, H. & Lawton, K. A. Organization of GC/MS and LC/MS metabolomics data into chemical libraries. J Cheminform 2, 9, https://doi.org/10.1186/1758-2946-2-9 (2010).
Rafikova, O. et al. Metabolic Changes Precede the Development of Pulmonary Hypertension in the Monocrotaline Exposed Rat Lung. PLoS One 11, e0150480, https://doi.org/10.1371/journal.pone.0150480 (2016).
Brown, M. V. et al. Cancer detection and biopsy classification using concurrent histopathological and metabolomic analysis of core biopsies. Genome Med 4, 33, https://doi.org/10.1186/gm332 (2012).
Worley, B. & Powers, R. Multivariate Analysis in Metabolomics. Curr Metabolomics 1, 92–107, https://doi.org/10.2174/2213235X11301010092 (2013).
Sarret, C. et al. Deleterious mutations in ALDH1L2 suggest a novel cause for neuro-ichthyotic syndrome. NPJ Genom Med 4, 17, https://doi.org/10.1038/s41525-019-0092-9 (2019).
Acknowledgements
This work was supported by NIH R01 grant DK054388 (to S.A.K.). The authors thank Dr. David Horita for carefully reading the manuscript.
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Conceptualization (S.A.K.); the overall study design and generation of knockout mice (N.I.K. and S.A.K.); conducting experiments (N.I.K., J.S., P.P. and B.F.); histological examination (K.L.H.); metabolomic data analysis and interpretation (S.S., N.I.K. and S.A.K.); bioinformatic analysis of nanostring data (X.D.). All authors contributed to the discussion of results and manuscript preparation.
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Krupenko, N.I., Sharma, J., Pediaditakis, P. et al. Cytosolic 10-formyltetrahydrofolate dehydrogenase regulates glycine metabolism in mouse liver. Sci Rep 9, 14937 (2019). https://doi.org/10.1038/s41598-019-51397-1
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DOI: https://doi.org/10.1038/s41598-019-51397-1
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