Abstract
Exposure to ambient air particulate matter (PM2.5) is well established as a risk factor for cardiovascular and pulmonary disease. Both epidemiologic and controlled exposure studies in humans and animals have demonstrated an association between air pollution exposure and metabolic disorders such as diabetes. Given the central role of the liver in peripheral glucose homeostasis, we exposed mice to filtered air or PM2.5 for 16 weeks and examined its effect on hepatic metabolic pathways using stable isotope resolved metabolomics (SIRM) following a bolus of 13C6-glucose. Livers were analyzed for the incorporation of 13C into different metabolic pools by IC-FTMS or GC-MS. The relative abundance of 13C-glycolytic intermediates was reduced, suggesting attenuated glycolysis, a feature found in diabetes. Decreased 13C-Krebs cycle intermediates suggested that PM2.5 exposure led to a reduction in the Krebs cycle capacity. In contrast to decreased glycolysis, we observed an increase in the oxidative branch of the pentose phosphate pathway and 13C incorporations suggestive of enhanced capacity for the de novo synthesis of fatty acids. To our knowledge, this is one of the first studies to examine 13C6-glucose utilization in the liver following PM2.5 exposure, prior to the onset of insulin resistance (IR).
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Discover the latest articles, news and stories from top researchers in related subjects.Introduction
Exposure to ambient air particulate matter with fine (PM2.5) and ultrafine (PM<2.5) aerodynamic diameter generated from anthropogenic sources, is associated with adverse effects on human health1. It has been established that PM2.5 primarily derived from stationary and traffic-related combustion sources trigger inflammatory stress responses associated to chronic obstructive pulmonary disease (COPD), asthma and cardiovascular conditions2,3,4. Recent studies have highlighted the importance of air pollution exposure in potentiating the risk of metabolic diseases such as Type 2 Diabetes (T2D)5 and metabolic abnormalities6,7,8. T2D is, indeed, a major driver of cardiovascular diseases9 and susceptibility to T2D may represent an important but underappreciated mediator of long term risk in response to air pollution exposure. Epidemiologic studies have demonstrated association between exposure and insulin resistance (IR) and T2D10. In addition, controlled exposure studies have uncovered a stereotypical response to air pollution, such as excessive hepatic gluconeogenesis, fasting and post-prandial hyperglycemia, abnormalities in triglyceride lipoproteins, hepatic steatosis and inflammation11. In fact, we as well as other researchers have demonstrated the important role air pollution plays in non-alcoholic steatohepatitis and fatty liver dysfunction (NAFLD)12,13,14.
In this study we examined metabolic effects in response to sub-chronic (4 months) PM2.5 exposure by 13C6-glucose tracing in mouse livers using stable isotope resolved metabolomics (SIRM) analysis. It was of particular interest to study these effects at sub-chronic time point as opposed to chronic exposure, prior to established phenotype of insulin resistance15. SIRM is a recognized approach that allows analysis of metabolic network analysis based on the tracer atom labeling patterns of numerous metabolites16. Our results show PM2.5 treatment reduced glycolysis and the Krebs cycle, but enhanced the oxidative branch of the pentose phosphate pathway (OxPPP) as well as data suggestive of increased fatty acid synthesis. These metabolic changes are similar to those observed in IR and T2D; hence, may be responsible for the deleterious health effects associated to PM2.5 exposure and the development of metabolic disorder.
Results
Decreased hepatic glycolysis in PM2.5 exposed mice
Previous studies show alteration of glucose utilization, including glycolysis, in the livers of animals and patients with IR or T2D17, and association of PM2.5 exposure to development of T2D and metabolic syndrome5. The IC-FTMS based SIRM analysis of the glucose metabolism in livers of PM2.5 exposed and filtered air (FA) exposed control mice produced 128 common peaks which were assigned to various metabolites related to cellular energetic pathways such as glycolysis, TCA cycle and nucleotide metabolism. Principal component analysis (PCA) of the concentration of unlabeled or total 13C labeled metabolites showed that samples of 16 weeks of exposure to PM2.5 separated from those of the control exposure to FA (Supplementary Fig. 1). PCA analysis results indicate PM2.5 altered the metabolism of glucose and other fuel sources in the liver. Hence, we examined the incorporation of 13C from uniformly 13C labeled glucose (13C6-Glc) into the glycolytic pathway in mice exposed to PM2.5 (Fig. 1a). IC-FTMS analysis exhibited a decrease in 13C6-glucose-6-phosphate, total G6P, and fructose-6-phosphate (F6P) levels in the livers of PM2.5 exposed mice as shown in Fig. 1b,c. Total levels of subsequent glycolytic intermediates, as well as 13C incorporation into fructose-1,6-bisphosphate (F1,6P), 3-phosphoglycerate (PG), and lactate also diminished based on both IC-FTMS and GC-MS analysis (Fig. 1d–f, and Supplementary Fig. 2a). These data are consistent with compromised hepatic glycolysis in PM2.5 compared to FA exposed mice.
It is interesting to note that the levels of both 13C-glycogen and unlabeled glycogen in the liver were significantly reduced in PM2.5 exposed mice based on NMR analysis as shown in Fig. 2a,b and Supplementary Fig. 5. The depletion of 13C-glycogen, together with decreased glycolysis, points to attenuated glycogen synthesis and thus decreased glycogen deposition in the liver.
PM2.5 exposure increases the oxidative branch of the pentose phosphate pathway
Next, we examined 13C incorporation from 13C-glucose into PPP. IC-FTMS analysis showed a nonsignificant increase in 13C labeled ribose-5-phosphate (R5P) and 13C5-sedoheptulose-7-phosphate (S7P) levels along with a boost of both total and 13C labeled phosphoribosyl pyrophosphate (PRPP) levels in PM2.5 exposed mice, as depicted in Fig. 3a,b,d. Consistent with PRPP being the ribosyl donor of nucleotide synthesis, we observed a significant increase in 13C5-ATP (Fig. 3c) in PM2.5 exposed livers, which presumably represent 13C5-ribose in ATP18. Together, these results support increased carbon flow from glucose to ATP via PPP. Moreover, we noticed depletion of unlabeled (12C) ATP in mouse livers exposed to PM2.5 (Fig. 3c), which could result from decreased glycolysis and compromised Krebs cycle (see below).
The PPP consists of two arms, nonOxPPP (Fig. 1a) and oxidative (OxPPP), which produces R5P and NADPH. The substantial rise in 13C5-S7P and total NADPH levels in PM2.5 versus FA exposed mice livers (Fig. 3d,e; cf. Fig. 1, scheme) is consistent with enhanced OxPPP presumably in response to ROS. We also measured the incorporation of 13C from glucose into antioxidant glutathione (GSH) by IC-FTMS and found that both total and 13C labeled GSH, were reduced (Fig. 3f) in PM2.5 exposed mice despite NADPH accumulation. This suggests compromised GSH synthesis capacity leading to inadequate maintenance of redox homeostasis.
Dysregulation of the hepatic Krebs cycle in PM2.5 exposed mice
We have previously demonstrated marked abnormalities in mitochondrial structure in response to PM2.5 exposure15. Therefore, we examined livers of PM2.5 and FA exposed mice for Krebs cycle intermediates and 13C incorporation from 13C6-glucose (Fig. 4a). Entry of 13C6-glucose derived carbons into the Krebs cycle via acetyl-CoA results in 13C2 (m + 2) labeling of citrate. 13C2-citrate was significantly reduced in PM2.5 exposed livers based on IC-FTMS and GC-MS analysis as shown in Fig. 4b and Supplementary Fig. 2b, respectively. We also observed reduced 13C2 labeling of Krebs cycle intermediates downstream of citrate, including succinate, fumarate and malate (Fig. 4c–e). We measured the levels of 13C2-aspartate as a surrogate metabolite for oxaloacetate (OAA), since OAA is highly labile and difficult to measure directly. 13C2-aspartate was significantly reduced in PM2.5 exposed livers (Fig. 4f). In addition, IC-FTMS analysis of 13C3 (m + 3) intermediates (Supplementary Fig. 3) suggests decreased entry from 13C3-pyruvate into the Krebs cycle via pyruvate carboxylation19. Despite the decrease in 13C6-glucose derived Krebs cycle metabolites, total unlabeled Krebs cycle intermediates were unchanged, with the exception of malate and aspartate, which decreased in PM2.5 liver.
Increased hepatic lipogenesis in PM2.5 exposed mice
One of the hallmarks of IR and T2D is the enhanced conversion of glucose into lipids20. The Krebs cycle-derived citrate is a precursor to fatty acid synthesis. Citrate is exported from the mitochondria by the mitochondrial citrate carrier (CIC) into the cytoplasm, where it is lysed to OAA and acetyl-CoA by the enzyme ATP citrate lyase (ACLY, see Fig. 4a). Acetyl-CoA then participates in de novo fatty acid synthesis with palmitate being the final product21. Therefore, we examined the incorporation of glucose derived 13C into palmitate by GC-MS. Despite the overall reduction in glycolysis and citrate synthesis, we detected increased total enrichment of 13C into palmitate (Fig. 5a). In addition, de novo fatty acid synthesis, which is reflected by enrichment of 13C-labeled acetyl units (derived from 13C glucose) into palmitate, significantly increased in PM2.5 exposed livers (Fig. 5b).
Discussion
Numerous epidemiologic studies and controlled exposure studies in both humans and animal models have demonstrated that exposure to PM2.5 induces the metabolic syndrome22. Indeed, we and others had previously demonstrated obvious IR phenotype in mice exposed chronically for 10 months15 and sub-chronically for 10–17 weeks. However, the latter was in mice additionally receiving high fat diet, co-exposure to nickel, or some genetic background, which significantly exacerbated the phenotype11,23,24. Conversely, at sub-chronic exposure for 3 months or less in similar experimental conditions to this study (fasting time and diet), IR phenotype was not obvious with no change in body weight and glucose tolerance profile24,25. Consequently, the IR phenotype in our animal model had resulted from chronic exposure alone or sub-chronic exposure to PM2.5 when accompanied by other environmental or biological stressor.
We have previously demonstrated an increase in expression of PEPCK, PGC1a and defects in the hepatic insulin-AKT-FoxO1 signaling pathway resulting in overt hyperglycemia and IR in response to PM2.5 inhalation11,26. This study sought to determine if metabolic pathway abnormalities in the liver precede the development of overt IR and T2D by examining changes in metabolites of intermediary metabolism from the livers of mice exposed to PM2.5 prior to the development of IR and T2D (Supplementary Fig. 4). Hence, we performed 13C stable isotope tracer approach to determine how PM2.5 exposure alters glucose utilization in the liver prior to IR and T2D development. We showed that PM2.5 exposure leads to changes in hepatic metabolism associated with T2D and IR, even before detecting evidence of their manifestation. Previous studies in cardiac tissues from mice exposed to acute PM2.5 showed reduced glycolysis27. In agreement, we observed depletion of 13C-labeled and total (12C + 13C) glycolytic products including lactate from the livers of mice exposed to PM2.5. Our data suggests that exposure to PM2.5 results in a decreased hepatic glycolytic activity derived from glucose and non-glucose precursors like glycogen.
13C incorporation into Krebs cycle intermediates were also reduced, signifying compromised Krebs cycle capacity. Previous studies exhibit increased anaplerotic input of non-carbohydrate substrates such as propionate, lactate or glucogenic amino acids into the Krebs cycle in diabetic rodents and humans28. This led to increased gluconeogenesis via the pyruvate carboxylase/PEPCK pathway and increased hepatic glucose output. Thus, it is not surprising that diabetes is associated to reduce oxidation of glucose via the Krebs cycle29,30. Our data supports that even prior to hyperglycemia, there was a reduction in glucose oxidation while anaplerosis was also attenuated based on m + 2 and m + 3 labeling of the Krebs cycle products. Furthermore, the majority of unlabeled Krebs cycle products did not change (Fig. 4), which could result from the replenishment by unlabeled substrates such as amino acids, other non-carbohydrate sources, and glycogen in metabolism adapted to prolonged fast31. A common finding in previous reports is increased accumulation of amino acids in the blood and the lungs, and dysregulation of amino acids in the liver, testis and heart after exposure to PM2.527,32,33,34,35,36. In the context of unlabeled sources of carbons, the data supports accelerated use of endogenous aspartate and malate in response to PM2.5 for PEP production by PEPCK as previously observed during decreased availability of glycolytic precursors37,38 and as is suggested by previously reported increased aspartate utilization in the lungs after acute PM2.5 exposure34. In our study, it seems plausible that dysfunctional glucose metabolism is balanced out by use of alternative fuels. Of note is the reduced glycogen synthesis from glucose in mice expose to PM2.5 (Fig. 2) which is characteristic of IR in agreement with our previous studies8. The depletion of unlabeled glycogen suggests reduced synthesis from sources other than glucose and/or enhanced glycogen catabolism (glycogenolysis) in PM2.5 exposed mice, either of which could increases glucose disposal into the circulation39,40. Moreover, reduced glycogen synthesis may be linked to increased fatty acid synthesis41. Accumulation of palmitate may inhibit glycogen production while mediating hepatic insulin resistant phenotype42.
Nevertheless, despite the reduced levels of the 13C-citrate precursor, incorporation of 13C into fatty acids was enhanced. This suggests that 13C-citrate was depleted in part due to its enhanced use in de novo fatty acid synthesis. Our results on fatty acid synthesis are in agreement with previously reported systemic imbalance of lipid homeostasis after exposure to PM1043, in NAFLD44, and increased blood lipids after chronic exposure to PM2.536. Indeed, at a late stage of progression to NASH or T2D, the mitochondrial respiration, ATP synthesis and rate of lipid oxidation in the liver is compromised45,46, which can impair insulin signaling and increase inflammation47,48. How this changes occur is ukknonwn, nevertheless, we presented evidence that a metabolic effect of PM2.5 exposure is compromised oxidation of glucose compensated by the use of alternative fuels. In line with our results, reported effects of acute PM2.5 exposure are decreased ATP in cardiomyocytes, decreased of fumarate accumulation in the liver, and the lungs27,33,34,49. However, we recognized that our assessment is limited to the liver metabolism of a single time point, that is insufficient to describe without ambiguity the kinetics and directionality of the metabolic reactions.
Interestingly, an increase in both branches (OxPPP and nonOxPPP) of the PPP is likely a consequence of the PM2.5 exposure. OxPPP produces R5P and NADPH, whereas nonOxPPP is reversible and switches between R5P generation from glycolytic intermediates or generation of glycolytic intermediates from R5P coming from OxPPP. This allows cells to alternate between R5P or NADPH and R5P production. Thus, OxPPP could be the predominant PPP when NADPH is needed for biosynthetic reactions and in response to oxidative stress. Therefore, increased NADPH accumulation suggests increased OxPPP, although we cannot rule out changes to other sources of NADPH. For example, recent reports suggest that malic enzyme (ME1) supplied NADPH for lipogenesis in prediabetic liver50. The requirement of NADPH by fatty acid synthesis would reduce NADPH availability for redox homeostasis. In line with NADPH clearance by reductive biosynthesis, we observed decreased de novo synthesis and total GSH. Particulate matter shows oxidative potential, and many studies note that exposure to the constituents of PM2.5 or PM0.1 has deleterious effects on the liver via reactive oxygen species (ROS) production51; black carbon nanoparticles52,53, graphene oxide54, TiO nanoparticles55, nickel56 and even the PM2.5 mixture57,58, have been shown to promote liver damage via ROS. In addition, previous studies showed that acute exposure to PM2.5 increased ROS in cells and decreased levels of GSH in the liver with similar results obtained for chronic exposure33,59,60. Therefore, our studies support compromised GSH synthesis as potentially an additional mechanism for the increase in oxidative stress following chronic exposure to PM2.5.
The mice exposed to PM2.5 showed no differences relative to filtered air exposed mice with regard to weight change or glucose tolerance at this early time point (Supplementary Fig. 4). Therefore, we assert to show that metabolic changes precede IR/diabetes onset in PM2.5 exposed mice. Indeed, previously published results showed redox imbalance in kidney of mice chronically exposed to PM2.5 and a similar metabolic phenotype after acute exposure in cardiac tissue and hepatic cells that the one reported here for the liver27,61,62. We acknowledge a limitation in our study is that molecular bases of metabolic dysregulation cannot be identified, a subject matter of future research. However, our results suggest an insight into the mechanism; the early responses of attenuated glycolysis, the Krebs cycle, and GSH synthesis, as well as increased lipogenesis, induce an imbalance of reductive capacity in the liver, leading to disrupted redox homeostasis in the liver of PM2.5 exposed mice.The dysrupt redox homeostasis could in turn lead to inflammation, increased fibrosis, and lipid steatosis in liver due to increased ROS and the subsequent development of IR and T2D12,63,64,65,66.
Materials and Methods
Animals
Seven-week-old male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME) and were equilibrated for 2 weeks before exposure. All mice were housed in cages with normal chow diet at 21 °C on a 12-h. light/12-h. dark cycle with free access to water and food. The protocols and the use of animals were approved by and in accordance with Animal Care and Use Committee (IACUC) at University of Maryland (protocol number 1113011) and Johns Hopkins University (protocol number M013H134), Baltimore. The performance of all experiments followed the recommendations and guidelines from IACUC, including all the methodlogy and procedures relevant to mice experimentation in this research.
Ambient whole-body inhalation protocol
C57BL/6J mice were exposed to filtered air (FA) or concentrated ambient PM2.5 in a mobile trailer located on the campus of the University of Maryland, Baltimore. The concentrated PM2.5 was generated using a versatile aerosol concentration enrichment system (VACES) as described previously67. Mice were exposed to concentrated PM2.5 particles for 6 hours per day, 5 days per week for a total of 16 weeks. The control group (mice exposed to FA) in the experiment were exposed to an identical protocol with all PM2.5 particles removed by a high-efficiency particulate-air filter positioned in the inlet valve. The average concentrations of PM2.5 during the exposure period were 8.7 ± 2.6 μg/m3 in the ambient air and 62.5 ± 21.3 μg/m3 in the concentrated PM chamber. The mean PM2.5 levels (±SD) in the current study (62.5 ± 21.3 μg/m3) were similar within standard deviation to our prior report (69.6 ± 48.4 μg/m3) of sub-chronic 3-month exposure and co-exposure with nickel (Ni)24. The chemical composition of the PM2.5 mixture in this study is unavialable, however a mixture in a near by site was characterized two years ealier68.
Intraperitoneal glucose tolerance test protocol
A week before the end of the exposure period we performed a previously reported intraperitoneal glucose tolerance test (IPGTT) procedure15. Glucose tolerance test required mice fasted for 12 hours (including the mice we did the 13C-glucose injection), which results in normal glucose use in the liver69. Briefley, mice were weighted and then injected intraperitoneally with glucose (2 mg/kg body weight). Blood samples were collected through the tail vein and glucose concentrations were measured before and 30, 60, 90, and 120 min after the injection on an Elite Glucometer (Bayer, Leverkusen, Germany). Supplementary Fig. 5 reports body weights, IPGTT plot, and area under the curve calculated using GraphPad software with standard error computed (n = 10).
13C6-glucose administration and tissue harvest
On the following day of the final exposure, mice were injected intraperitoneally with 13C6-glucose (Cambridge Isotopes Laboratories) according to the protocol described by Fan et al. with modification70. 80 μl (20 mg) of 13C6-glucose in PBS were injected at 15 min. intervals for three times to mice fasted for 12 hours. Time of injection was recorded and blood was collected before and after injection. Liver tissues were collected at 45 min. after the first injection (15 min. after the last injection) and frozen immediately (within 5 min. of necropsy) in liquid N2.
Sample preparation
Quenching and extraction of liver samples (left lobules) were performed as previously described71. Briefly, tissues were pulverized into <10 µm powder in liquid nitrogen using a Spex Freezer Mill (SPEX SamplePrep, Metuchen, NJ, USA). Metabolites and proteins were then extracted in a final 2:1.5:1 ratio of acetonitrile:H2O:chloroform. Protein content was determined using the Pierce BCA method (Thermo Fisher Scientific, Rockford, IL) for normalizing the metabolite concentration.
The polar fractions were aliquoted and lyophilized for NMR and IC-FTMS analysis. The NMR fractions were further deproteinated in 80% acetone solution (100 µL ice-cold nanopure water and 400 µL ice-cold 100% acetone), incubated at −80 °C for 30 min., followed by centrifugation at 4 °C, 14,000 rpm for 20 min. The supernatant was lyophilized.
Gas chromatography-mass spectrometry
Polar and non-polar (for fatty acids) fractions were isolated as previously described72,73,74. In brief, livers were homogenized in 0.9% NaCl, centrifuged and a modified Folch extraction performed using 2:1:0.2 0.9% chloroform:methanol:NaCl for non-polar metabolites. For polar metabolites, livers were homogenized in 80% methanol, freezed thawed 3 times, centrifuged and supernatant collected. Fatty acids were saponified and converted to their methylated derivatives. Palmitate and its isotopomers were monitored at m/z 270–286 by gas chromatography-mass spectrometry (GC-MS). The enrichment of acetyl-CoA units and de novo palmitate synthesis were determined as previously described73,74. Briefly, for acetyl CoA enrichment by the formula of m4/m/2 = (n-1)/2(p•q), where n equals the number of acetyl units, for palmitate = 8; p is the 13C labeled precursor acetate fraction and q is the 12C acetate fraction. For polar metabolites samples were methoximated, derivatized using BSTFA and analyzed by GCMS74. The m/z for the following metabolites and their isotopologues were monitored: lactate 219–222 m/z, m + 3 = 222; 3PG 459–462 m/z; citrate 465–471 m/z, m + 2 = 467; fumarate m/z = 245–249, m + 2 = 247; succinate m/z = 247–251, m + 2 = 249; Malate m/z = 335–339, m + 2 = 337; Aspartate m/z = 334–338, m + 2 = 336, 3PG m/z = 459–462, m + 3 = 462. Data were analyzed using Mass Hunter (Agilent, USA) and abundance corrected using ISOCOR.
Nuclear magnetic resonance (NMR)
1D 1H and 1H (13C) HSQC (heteronuclear single quantum coherence) NMR analyses of polar extracts reconstituted in D2O (>99.9%, Cambridge Isotope Laboratories, MA) containing 0.1 mM EDTA (Ethylenediaminetetraacetic acid, Sigma Aldrich, St. Louis, MO) and 0.5 mM d6-2,2-dimethyl-2-silapentane-5-sulfonate (DSS) (Cambridge Isotope Laboratories, Tewksbury, MA) as internal standard were performed on a DD2 14.1 Tesla NMR spectrometer (Agilent Technologies, CA) equipped with a 3 mm inverse triple resonance HCN cryoprobe. 1D 1H spectra were acquired with standard PRESAT pulse sequence at 15 °C. A total of 16384 data points were acquired with 2 s. acquisition time, 512 transients, 12 ppm spectral width, and 4 s. recycle delay time. The spectra were then linear predicted and zero filled to 128k points and apodized with 1 Hz exponential line broadening. 1D HSQC spectra were recorded with 13C adiabatic decoupling scheme for broad range decoupling during proton acquisition time of 0.25 s. 1796 data points were collected for each transient and a total of 1024 transients were acquired with 12 ppm spectral width. The HSQC spectra were then apodized with unshifted Gaussian function and 4 Hz exponential line broadening and zero-filled to 16k data points before Fourier transformation. Metabolites were assigned by comparison with in-house75 and public NMR databases. Metabolite and their 13C isotopomers were quantified using the MestReNova software (Mestrelab, Spain) by peak deconvolution. The peak intensities of metabolites obtained were converted into nmoles by calibration against the peak intensity of DSS (27.5 nmoles) at 0 ppm for 1 H spectra. For HSQC spectra, 13C-3-lactate was quantified by the two 13C satellite peaks in the 1D 1H spectra, which was used as the internal calibration standard for quantifying other assigned HSQC signals, as described previously76. Quantified metabolites were normalized to total protein weight for each sample.
Ion chromatography-fourier transform mass spectrometry (IC-FTMS)
Polar extracts for IC-FTMS were reconstituted in 20 µL ultrapure water (EMD Millipore) of which 10 µL was used for IC-FTMS. All IC-FTMS analyses were performed on a Dionex ICS-5000+ ion chromatography interfaced to a Thermo Fusion Orbitrap Tribrid mass spectrometer (Thermo Fisher Scientific) as previously described77, with the exception that we used a scan range of 80 to 700 m/z. Isotopologue peak areas were integrated and exported to Excel via the Thermo TraceFinder (version 3.3) software package. Natural abundance correction of peak areas was performed as described previously78. Fractional enrichment was calculated as the percentage of the natural abundance corrected signal of each isotopologue from the sum of all isotopologues for given metabolite and averaged across all replicates. Quantification for selected metabolites was achieved by using an external standard mixture and normalization to protein weight. Principal component analysis (PCA) used the pca function of the MATLB software version R2018a. The statistical parameters of PCAs are in Supplementary Tables (1 and 2). Pair way comparation analysis used the Benjamin-Hochberg correction for multiple testing and the Student t-test to calculate p-values of 12C and 13C-metabolites analyzed by IC-FTMS as shown in the figures (FDR ≤ 0.1).
Data availability
The data that support the findings of this study are available upon request to anyone with a public interest. Please send and email to the corresponding author where you clearly indicate your affiliations.
Change history
16 March 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Acknowledgements
The research reported in this publication was supported in part by the National Institute of Environmental Health Sciences (NIEHS) of the National Institutes of Health (NIH) under Awards Numbers U01ES026721 (to SB and SR), P30ES026529 (to T.W.M.F) and Research Supplements to Promote Diversity in Health-Related Research award number 3U01ES026721-02S1 (to H.R.C.). AM was supported by National Heart, Lung, and Blood Institute grant HL130516. T.W.M.F would also like to acknowledge the support of 1U24DK097215-01A1 from NIDDK. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health (NIH) or of the National Institute of Environmental Health Sciences (NIEHS).
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H.R.C. carried out ICFT-MS and NMR metabolite experiments, performed analysis, interpreted results, drafted the manuscript and supervised the project; G.D.G. performed and analyzed GC-MS metabolite experiments; X.R. and T.E.S. carried out the mice exposure, all animal related experiments and contributed to experimental design; S.Q. and M.O.W. collected mass spectrometry data and contributed to its analysis; P.L.; collected and assisted in the analysis of NMR data; B.P. contributed to data analysis; T.W.M.F. and G.D.G. drafted and improved the manuscript, data interpretation, study design and generation of mass spectrometry data; A.M., X.R., S.R. and H.R.C. discussed results and edited manuscript; S.B. and S.R., were the principle investigators, conceived the idea and led the study design.
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Reyes-Caballero, H., Rao, X., Sun, Q. et al. Air pollution-derived particulate matter dysregulates hepatic Krebs cycle, glucose and lipid metabolism in mice. Sci Rep 9, 17423 (2019). https://doi.org/10.1038/s41598-019-53716-y
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DOI: https://doi.org/10.1038/s41598-019-53716-y
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