Abstract
Temperature is a key factor that affects all levels of organization. Minute shifts away from thermal optima result in detrimental effects that impact growth, reproduction and survival. Metabolic rates of ectotherms are especially sensitive to temperature and for organisms exposed to high acute temperature changes, in particular intertidal species, energetic processes are often negatively impacted. Previous investigations exploring acute heat stress have implicated cardiac mitochondrial function in determining thermal tolerance. The brain, however, is by weight, one of the most metabolically active and arguably the most temperature sensitive organ. It is essentially aerobic and entirely reliant on oxidative phosphorylation to meet energetic demands, and as temperatures rise, mitochondria become less efficient at synthesising the amount of ATP required to meet the increasing demands. This leads to an energetic crisis. Here we used brain homogenate of three closely related triplefin fish species (Bellapiscis medius, Forsterygion lapillum, and Forsterygion varium) and measured respiration and ATP dynamics at three temperatures (15, 25 and 30 °C). We found that the intertidal B. medius and F. lapillum were able to maintain rates of ATP production above rates of ATP hydrolysis at high temperatures, compared to the subtidal F. varium, which showed no difference in rates at 30 °C. These results showed that brain mitochondria became less efficient at temperatures below their respective species thermal limits, and that energetic surplus of ATP synthesis over hydrolysis narrows. In subtidal species synthesis matches hydrolysis, leaving no scope to elevate ATP supply.
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Introduction
Temperature exerts a profound effect on all levels of organization; with no single organism, capable of withstanding the full spectrum of temperatures across the biosphere1,2. This is true of ectotherms, which lack active thermoregulatory processes and especially true for intertidal species which experience large fluctuations in ambient temperature over the course of a day1. With anthropogenic climate change shifting mean and extreme temperature patterns, understanding the basis of thermal tolerance and thermal adaptation is essential3,4,5. Temperature typically promotes a near exponential rise in metabolic rate until a critical threshold is reached, which is then followed by a sharp decline6. What ultimately mediates this metabolic collapse at high temperature remains controversial, and oxygen limitation has been proposed to underpin thermal tolerance in aquatic ectotherms7,8. However, this concept is debated, as underlying literature finds support for and against its application8,9,10,11,12.
Perhaps the most central role of metabolism is to maintain a tight balance between ATP production and consumption. In almost all animals that live independent from a host, mitochondria are the predominant source of ATP, and oxidative phosphorylation (OXPHOS) provides ~ 90% of the cellular ATP, around 15 times more than that generated via fermentative glycolysis13,14. Considerable work has focused on the mitochondrion’s role, specifically cardiac mitochondria, as the linchpin for thermal tolerance15. With rising temperature, the fraction of oxygen (O2) flux and carbon substrate consumed by mitochondria escalates, yet the energy released is increasingly directed toward an apparent futile cycle, or “leak” of protons across the inner mitochondrial membrane14,15,16.
Rising temperatures increase cellular ATP demands6, yet mitochondrial efficiency and outright ATP production may decline, even with saturating O215,17. In sum, a mismatch between an organism's capacity to meet increasing ATP demands must occur. While considerable focus has been placed on respiration and ATP production, few have considered how these relate to rates of ATP hydrolysis at high ambient temperatures. This relatively simplistic concept of balance in cellular ATP-economics has yet to be followed in the contexts of temperature. We have the ability to measure ATP production as well as a simplistic measure of ATP hydrolysis under variable thermal stresses. This allowed us to generate a clearer picture with regard to energetic economy at high temperatures.
The role of mitochondria in thermal tolerance has mainly been assessed in liver and cardiac tissues, and hearts have been proposed to fail first, often with minimal increase in temperature15,18,19. However, nervous tissue is also excitable and may respond similarly with minor temperature fluctuations, and result in catastrophic loss of brain function20,21,22. The brain is also highly aerobic, as an excitable tissue with high sustained energetic demands23,24,25,26,27. Moreover, the resting brain is the greatest energy consumer in vertebrates, and even within ectotherms the brain may use upwards of 20% of resting O2 demands28. Action potentials and consequent restoration of ion balance consumes up to 80% of total ATP in active neurons and these pathways, along with synaptic transmission, are thermally sensitive28,29.
Intertidal marine ectotherms typically experience large diurnal and tidally mediated temperature fluctuations, relative to subtidal species, and consequently show physiological adaptations to survive thermal stress31. Adaptations can be detected through contrasting related species of distinct ecotypes32, and here we employ the New Zealand triplefin fish (Family: Tripterygiidae) model. We tested three species, Bellapiscis medius, Forsterygion lapillum and Forsterygion varium that respectively occupy niches within the high (0–5 m) and mid (1–10 m) intertidal zones and subtidal zones (4–15 m) and show distinct critical thermal maxima (CTmax) that reflect their thermal habitats (Fig. 1)33,34. Earlier work on triplefin species has shown clear differences in heart mitochondrial efficiency and stability at high temperatures, with the intertidal B. medius maintaining the lowest leak respiration rates compared with the subtidal F. varium and F. malcolmi33. The aim of the present study was to investigate the effects of temperature on the ATP dynamics and overall mitochondrial function of three spatially diverse triplefin species. We hypothesised that mitochondrial efficiency would decrease as ambient temperature increased, and more specifically, that overall energetic balance would become closely matched as ATP supply is diminished and is unable to meet the ever-increasing demand. As such, we expect the rockpool exclusive B. medius to have the greatest mitochondrial efficiency at high temperatures, with sustained rates of ATP production above ATP demands, compared to the low intertidal F. lapillum and subtidal F. varium. Therefore, this work will provide a greater understanding into the underlying factors of thermal tolerance.
Materials and methods
Ethics
The research described conforms to the recommendations of the New Zealand Animal Welfare Act of 1999. All experiments were conducted under the approval of the animal ethics committee and animal welfare officer of the University of Auckland, New Zealand (permit R001551). Study was undertaken in compliance with ARRIVE guidelines.
Animals and housing
The rock-pool exclusive and high intertidal species (Bellapiscis medius and Forsterygion lapillum, respectively) were caught using bait traps and hand nets in rock pools and off piers. Subtidal species Forsterygion varium were caught by SCUBA. All fish were collected around the greater Auckland region (− 36.081588, 174.598804). Fish were then transported to the facilities of the University of Auckland and held in 30 L aerated tanks with recirculating seawater at 18 ± 0.5 °C, 200 µm filtered, and 35 ± 1 ppt salinity. Fish were checked daily and fed ad libitum every 3 days with raw prawn meat. After at least a week of acclimation, fish were euthanized for experimentation. The three species were chosen for their differing habitat depth and thermal histories. B. medius is found at a depth range of 0–2 m and has a CTmax of 31.9 °C ± 0.0134, F. lapillum is found at depths of 0–10 m and has a CTmax of 31.3 °C ± 0.07, while F. varium is found at depths of 0–30 m. The CTmax values for F. varium are not available, but this species does not routinely encounter water temperatures as high as 25 °C33,34.
Tissue homogenization
Fish were euthanized via pithing of the spinal cord. The brain case was then removed and the brain was rapidly excised, weighed and placed into a marine fish specific ice-cold respiration buffer (MiR05; modified from Gnaiger, 2007; EGTA 0.5 mM, Lactobionic acid 60 mM, Taurine 20 mM, KH2PO4 10 mM, HEPES 20 mM, d-Sucrose 160 mM, BSA 1 g/L, pH 7.1 at 30 °C) at a 1:10 weight to volume dilution. Brain bundles were gently homogenized via suction and expulsion through a 10 ml homogenising syringe and two decreasing gauge needles (16 and 25).
Respirometry
Mitochondrial respiration assays were performed using Oroboros Oxygraph-2k respirometers (Oroboros instruments, Innsbruck, Austria). The oxygen standard was equilibrated to air levels according to chamber temperature (~ 290, 255 and 217 μM O2 at 15, 25 and 30 °C respectively) prior to experiments. Weight specific mitochondrial respiration flux (JO2) was calculated in real time using the negative time derivative of the O2 concentration and expressed as pmol O2 s−1 mg−1. Two assay protocols were used to track respiration as well as ATP dynamics at three temperatures (Fig. 2). Assay temperatures in this study represented the average seasonal habitat temperature, standard temperature and maximal temperature reached in rock-pools in summer (i.e., 15, 25 and 30 °C, respectively)34. A substrate-uncoupler-inhibitor-titration assay (SUIT) was used to test (i) mitochondrial components of the electron transport system (ETS) in a stepwise fashion, measured with the maximum O2 flux mediated by each titration, (ii) ATP production through OXPHOS and (iii) ATP dynamics of triplefin brain mitochondria (Fig. 2). Both assays differed by the addition of either ADP or ATP. Brain homogenate (~ 10 mg wet weight equivalent) was distributed equally between two parallel respirometry chambers and left until steady-state respiration was reached. Then, 2 mM final concentration of ADP (assay 1, one chamber) or ATP (assay 2, second chamber) was added to measure respiration rates supported by endogenous substrates (Routine state). The addition of ADP primed OXPHOS, while the addition of ATP forced hydrolysis rates. The NADH2-generating substrates pyruvate (5 mM), malate (2 mM), and glutamate (10 mM) were then added to stimulate CI-OXPHOS. Subsequently, succinate (10 mM) was added to stimulate complex II (CII) and allow the measurement of OXPHOS with the combined inputs of CI and CII (CI&CII-OXPHOS). Respiration attributed to proton leak (LEAK) was assessed with the addition of the ATPF0–F1 synthase inhibitor oligomycin (2.5 μM). The contribution of adenine nucleotide translocator (ANT) to LEAK was calculated as the difference prior and after the addition of the ANT inhibitor carboxyatractyloside (cAtr; 5 μM). Mitochondria were then uncoupled from OXPHOS with repeated titrations of carbonyl cyanide m-chloro phenyl hydrazone (CCCP; 0.5 μM titration steps) to determine the maximum ETS capacity without the limitation of the phosphorylating system (ATPF0–F1 and ANT). Finally, non-mitochondrial respiration was determined by the addition of the complex III inhibitor antimycin A (Ama; 2.5 μM) was titrated to inhibit respiration (Fig. 2).
ATP dynamics: assay and kinetics
Standard respiration media (MiR05) does not contain calcium (Ca2+) or sodium (Na+). As Na+ and Ca2+ salts are required to activate cellular ATPases; NaCl (5 mM) and CaCl2 (0.25 mM) were added before homogenate to reach maximum ATP hydrolysis rates. Maximum OXPHOS rates cannot be achieved in the absence of Mg2+, which is required for proper cellular function and stabilisation of ATP and ADP35. ATP and ADP kinetics were measured fluorometrically, as described elsewhere36,37,38,39. Briefly, as both ADP and ATP require Mg2+ for stabilisation, free Mg2+ [Mg2+] free within the O2K chamber was monitored using Magnesium-Green™ (5 mM). The fluorescent signal (470/530 nm; Ex/Em) was calibrated by two subsequent titrations of MgCl2 (1.25 mM each) so that (i) Mg2+ dependent reactions can be achieved and (ii) Mg2+-free ADP and ATP are stabilised. As binding varies with temperature, independent assays were run at each experimental temperature without sample to determine binding kinetics between ADP-Mg2+, ATP-Mg2+ and Mg2+-MgG. Post fluorescent signal calibration, ADP and ATP (Mg2+-free) were titrated stepwise (1.25–2.5–3.75–5–6 mM) (Supplementary Fig. S1). This allowed the construction of ADP and ATP binding curves to Mg2+. The ratio of the slopes between the two curves at working ATP/ADP concentrations for assays 1 and 2 were used to determine a fluorescence correction factor for each assay at each experimental temperature (Supplementary Fig. S1). The experimental ADP signals were subsequently multiplied by the correction factor to determine rates of net ATP production36,40. ATP hydrolysis rates were determined during the SUIT assay protocol following the addition of Oligomycin to inhibit the ATP synthase. Using this rate, we could calculate the overall ATP production rate through the sum of the net ATP production rate with the ATP hydrolysis rate. Overall production and hydrolysis rates provide insights into the condition of the tissue, but more physiologically relevant information is acquired from P/O ratios; the amount of ATP produced per molecule of oxygen consumed. Changes in this ratio across temperature are calculated by dividing the rate of overall ATP production by O2 consumption during CI&CII-OXPHOS.
Calculations and statistical analyses
Respiration data is presented as mean (n = 10 individuals) ± s.e.m (alternatives are otherwise stated). Respiratory control ratios (RCR) are calculated as (State 3-State 4)/State 3. State 3 corresponds to a more recently defined OXPHOS state when mitochondria are exposed to sufficient substrates and ADP, whereas state 4, or LEAK, is measured in the absence of ADP, or also in the presence of oligomycin (state 4o). In this present study on homogenates RCRs were calculated as (CI&CII-OXPHOS-LEAK)/CI&CII-OXPHOS (e.g., (State 3-State 4o)/State 3). The reserve respiratory capacity is calculated as CI&CII-OXPHOS—ETS. Prism (Vers.8) was used to conduct independent t-test between mitochondrial states when homogeneity of variance was verified. Two-way ANOVAs were performed to analyse the effect of temperature and species on respiration rates and ATP dynamics with a Greenhouse–Geisser correction when sphericity was not assumed. Tukey-Post Hoc tests were performed for pairwise comparison.
Results
Respiration assays
A SUIT protocol was applied to stimulate mitochondrial respiration in brain mitochondria. The respiratory flux at 15 °C, showed no differences among species for any of the mitochondrial states (Fig. 3). Differences were seen across all states at 25 °C (Fig. 3). Both F. lapillum and B. medius showed higher flux during CI-OXPHOS and CI&CII-OXPOS compared with F. varium (Fig. 3a,b; B. medius: CI-OXPHOS, p = 0.007; CI&CII-OXPHOS, p = 0.025; F. lapillum: CI-OXPHOS, p = 0.001; CI&CII-OXPHOS, p < 0.0001). ETS rates differed between all three species, with F. lapillum having the highest rates compared with B. medius and F. varium (p < 0.0001). Differences were seen between F. lapillum and B. medius at CI&CII-OXPHOS (Fig. 3b, 25 °C: p = 0.008) and LEAK (Fig. 3c, 25 °C: p < 0.0001; 30 °C: p = 0.008). ETS rates significantly differed between all species at 30 °C with F. varium displaying the lowest rate (p < 0.0001). CI-OXPHOS (p = 0.013) and CI&CII-OXPHOS (p = 0.001) also differed significantly with F. varium having the lowest rates compared with F. lapillum at 25 °C and 30 °C (Fig. 3a,b).
Complex II contribution to mitochondrial flux was calculated as CI-OXPHOS subtracted from CI&CII-OXPHOS (Fig. 4a–d). Differences were seen at 25 °C with F. lapillum showing greater CII contribution compared with B. medius (Fig. 4b,d; 25 °C, p = 0.026) and F. varium (Fig. 4b,d; 25 °C, p = 0.018). Reserve respiratory capacity was calculated as CI&CII-OXPHOS subtracted from ETS. This is a measure of excess capacity above CI&CII-OXPHOS available to the mitochondria. Reserve capacity increases for all three species between 15 and 25 °C, with F. lapillum having greater capacity compared to F. varium (Fig. 4e; 25 °C: p = 0.026). Excess capacity plateaus and decreases for both B. medius (Fig. 4e; F. varium. p = 0.002; F. lapillum, p < 0.0001) and F. varium (Fig. 4e; B. medius, p = 0.002; F. lapillum, p < 0.0001) at 30 °C, while it increases in F. lapillum with differences seen across all three species (Fig. 4e; B. medius and F. lapillum, p < 0.0001).
ATP assays
At all temperatures ATP production rates differed substantially from hydrolysis rates for all three species (Fig. 5a; p < 0.0001). At 30 °C overall ATP production rates decline from those at 25 °C for F. lapillum and B. medius but remain above the increasing ATP hydrolysis rates (Fig. 5a; 30 °C: p < 0.0001). Net ATP production rates decline sharply to closely match hydrolysis rates at 30 °C, and only remained marginally higher for B. medius (Fig. 5b; 30 °C: p = 0.011) and F. lapillum (Fig. 5b; 30 °C: p = 0.018) but were equivalent for F. varium (Fig. 5b; 30 °C: p = 0.8846).
Respiratory control and P/O ratios
Respiratory control ratios (RCRs) were calculated as (CI&CII-OXPHOS-LEAK)/CI&CII-OXPHOS and these represent the proportion of oxygen consumption commonly assumed to be coupled to ATP synthesis. High RCR values are indicative of tightly coupled mitochondria while low values represent dysfunctional mitochondria. Phosphate/Oxygen or P/O ratios were calculated as the rate of overall ATP production divided by O2 consumption during CI&CII-OXPHOS and provide a more accurate, dynamic measure of the amount of ATP produced per molecule of oxygen consumed in the background of ATP hydrolysis. At 25 °C the RCR for B. medius was significantly higher than that of F. varium (Fig. 5c; p = 0.0067). For P/O ratios, B. medius had a greater P/O ratio than that of F. varium at 30 °C (Fig. 5d; p = 0.0186). P/O ratios showed a significant decline from 25 to 30 °C in F. varium but not for the two other species (Fig. 5d; p = 0.0014).
Discussion
We demonstrate that brain mitochondrial efficiencies likely play a key role in thermal tolerance and that as temperature increases respiration and ATP production decreases leading to a tight energetic balance. Notably our approach permitted some comparison of ATP synthetic to hydrolysis rates, and the amount of ATP consumed increases as the amount of ATP produced declines. For the subtidal F. varium at 30 °C the balance between production and consumption of ATP sits on a knife’s edge (Fig. 5). While the intertidal species, B. medius and F. lapillum, showed declines in ATP production and consumption as temperature increased, the energetic surplus of ATP synthesis narrows yet remains in excess for these intertidal species at 25 and 30 °C.
Energetic balance in the brain
The role of neural function in setting the upper thermal limits has been largely ignored. Similar to the heart, the brain is an excitable tissue with high basal energetic demands23,24,25,26,27. The changes in mitochondrial efficiency and ATP dynamics experienced during heat stress will mediate dysfunction across the neural system and alter brain function. Early investigations have argued that neural function at the organ and cellular level may limit upper thermal tolerance11,16,30. Decreased control of ventilation and blood circulation within the brain was shown as temperature increases41, while at the cellular level, action potential conduction and synaptic transmission is sensitive to increasing temperatures in several studies42,43,44,45. Other recent studies have compared the acclimation responses of heart and brain in eurythermal teleost species. While both organs showed significant acclimation responses, a larger acclimation response was found in the brain30. In two crustacean species (Penaeus monodon and Astacus astacus), the cardiorespiratory system maintained O2 supply up to Tcrit suggesting temperature resistance of the heart; however, the generation and conduction of neuronal action potentials failed approaching Tcrit, suggest a greater thermal sensitivity of the nervous system relative to the heart in these species12.
The high energetic demands of the brain require a balance to be maintained between the production of sufficient ATP and its rapid consumption. This study is the first to attempt to formulate a measure of the dynamic energetic balance of ATP production and use, specifically, in terms of the mismatch between energy supply and demand during heat stress. Generation of action potentials involves rapid plasma membrane depolarization and requires repolarization using ATP-dependent ion pumps46. With alterations in channel properties, neuronal membranes become leakier with increasing temperature, and active ion repartition becomes more energetically costly47,48. Action potentials must also exceed a threshold and increased temperature decreases action potential amplitude and duration, decreasing propagation in excitable tissue48. An additional large ATP sink involves the extrusion of neurotransmitters into synaptic clefts to transmit signals, and this increases with heat stress44,49.
Here, we determined mitochondrial function and specifically, relatively simple ATP dynamics. These changed with temperature for all three species, and we observed a clear decrease in mitochondrial stability and capacity to meet ATP requirements as temperature increases. While we used brain homogenates, which may not represent in vivo ATP demands, it provides a proxy measurement and system to measure ATP synthesis and hydrolysis under equivalent conditions. Regardless, the subtidal species’ F. varium is the most sensitive to temperature and is at a point of no reserve ATP capacity, while the intertidal B. medius shows greater resilience to elevated temperatures.
Coupling of OXPHOS
OXPHOS rates increased with temperature for all species (Fig. 3a,b) and indicates increased O2 consumption. Leak rates from all three species also increased significantly from 15 to 30 °C with the greatest proportion of respiration flux resulting from LEAK in F. varium at 30 °C (Fig. 3c). These results align with previous work on permeabilized heart33 and skeletal muscle fibres50 from triplefin fish, where LEAK O2 flux also elevates at high temperatures. This supports the view that mitochondrial function and stability declines prior to CTmax15,17,33. Elevated O2 demand as OXPHOS efficiency decreases may become increasingly detrimental if sufficient O2 cannot be extracted from water. Notably, F. varium and F. lapillum, have lesser capacities to extract O2 than B. medius at low O2 partial pressures51. The synergistic effects of increasing temperature will increase the drain on O2 and substrate (e.g., glucose) to maintain nervous function.
We tested the integrity of the ETS complexes, at 15, 25 and 30 °C. Consistent with previous work33, ETS rates increased significantly from 15 to 30 °C in all three species (Fig. 3d); however, the greatest increase in flux was seen in the mid tide species F. lapillum (Fig. 3d). Reserve respiratory capacity, calculated as the difference between maximal O2 consumption (ETS) and basal respiration (CI&CII-OXPHOS) has been employed to provide an estimate of a tissues capacity to cope with increases in ATP demand52. Under “normal” conditions, mitochondria are thought to operate at a lesser fraction of their energetic capacities. For cells such as neurons that experience large fluctuations in energy demand on short timescales, the capacity to increase supply to meet demands are essential. The mid-tide species, F. lapillum, had the greatest reserve respiratory capacity at 25 and 30 °C compared with both F. varium and B. medius (Fig. 4e). Theoretically, this greater reserve capacity will provide F. lapillum a wider window to defend and buffer ATP supply following s conditions of stress. However, the elevated LEAK rate at both 25 and 30 °C for F, lapillum (Fig. 3c) indicate that less of the consumed oxygen is directed towards ATP production. Our measures of ATP synthesis reflect this effect in the contexts of P/O ratios (Fig. 5b).
ATP dynamics
Oxygen consumption is a traditional proxy or indirect measure of energy expenditure in aerobic organisms, as most ATP production occurs aerobically53,54,55. However, this assumption is compromised if OXPHOS is uncoupled. Only measures of ATP dynamics, or balance, can determine ATP production rates at elevated temperature and provide insight into function under physiological conditions. This study revealed that at 25 °C ATP production rates are elevated for the two intertidal species while it was diminished for F. varium. At 30 °C, and all three species showed significantly diminished ATP production rates and elevated ATP hydrolysis rates (Fig. 5). Work performed on the common New Zealand wrasse (Notolabrus celidotus) showed that the capacity of isolated cardiac mitochondria to efficiently produce ATP decreased at 25 °C, prior to temperature induced heart failure at 27.5 °C15,17. Work with isolated mitochondria from rat cardiomyocytes also revealed diminished ATP production at elevated temperatures and the eventual reversal of the ATP synthase at 43 °C, making the mitochondria a consumer of ATP17. Use of Phosphorus-31 NMR (31P-NMR) during acute heat shock measured the rapid decline in ATP at elevated temperatures in Tetrahymena ciliate species56,57, and the same technique has been used by others and revealed an immediate fall in ATP after brief exposure to sub-lethal heating26,57. While the initial ATP levels were recovered after 48 h, a prolonged exposure to heat stress would likely lead to irreversible loss of ATP and the eventual activation of necrotic pathways and death. In B. medius and F. lapillum, the exposure to elevated temperatures in rock pools occurs over several hours within a day34. While these are sub-lethal regarding their respective CTmax, the decreases in mitochondrial efficiency and ATP production can lead to the irreversible loss of function. Similarly, exposure to moderate heat stress in liver homogenates of juvenile Brown trout (Salmo trutta) induced lowered mitochondrial coupling and increased leak rates and the inferred insufficiency to maintain ATP homeostasis may have diminished food intake and suppressed growth58. As expected, ATP hydrolysis rates in this study were highest at 30 °C in all species (Fig. 5a). The temperature sensitive F. varium had the lowest rates of ATP production relative to ATP hydrolysis and this is reflected in the declining P/O ratios (Fig. 5a,b). However, at 30 °C all three species showed declines in the efficiency of brain homogenates to produce ATP above the ever-increasing hydrolytic rates of ATP. Prior to their respective CTmax, all species have decreased mitochondrial efficiency to adequately produce ATP, leading to growing imbalance between ATP demand and ATP production.
Mitochondrial efficiency can be defined by the organelles ability to efficiently transfer the free energy released from reducing pathways to ATP production. Traditionally P/O ratios have been used to quantify the number of ATP molecules produced per molecule of O2 consumed. Mechanistic P/O ratios have been previously calculated using end-point protocols in respiration assays55,59; while the use of MgG fluorescence has allowed the generation of more accurate “active” and “steady- state” P/O ratios. This technique was utilized in recent studies that have provided insight in terms of mitochondrial efficiency with changing temperatures across a range of species15,17,37,39. Traditional approaches were unable to produce P/O ratios that were informative about ATP dynamics, whereas another approach17 enabled a more dynamic assessment of production and hydrolysis of ATP. Increases in temperature mediated decreases in P/O ratios as mitochondria became less efficient at producing ATP per O2 consumed. At 25 °C there was a near 25% decrease in the P/O ratio from 15 °C in F. varium. This was further exacerbated at 30 °C where the P/O ratio was further decreased by up to 75% (Fig. 5b). This decline in P/O ratio means more O2 is required to sustain ATP production, this also requires a concomitant increase in other metabolic fuels, such as glucose.
The brain is predominantly aerobic, relying on steady supply of O2 and glucose to fuel its activity. With limited capacity for anaerobic metabolism, ATP production at elevated temperatures is time restricted and comes at a potentially greater long-term cost for the individuals. Comparing across species, B. medius has the greatest brain tissue glycogen stores compared with both F. lapillum and F. varium51. This paired with the higher P/O ratios at elevated temperatures provides B. medius with a greater capacity to function at elevated temperatures it may experience during the tidal cycle. Similar declines in P/O ratios have been shown in studies at elevated temperatures15,17. The common New Zealand wrasse (Notolabrus celidotus) showed decreased ATP production rate by cardiac mitochondria as temperature increased and showed depressed “active” P/O ratios at 32.5 °C15. The “steady- state” P/O ratios calculated by Ref.17 showed a decline in P/O ratio from 2.5 at 37 °C down to a negative P/O ratio at 43 °C for rodent heart mitochondria. At 25 °C, all three species could maintain sufficiently high P/O ratios while at 30 °C only the rock-pool exclusive B. medius was able to sustain a P/O ratio above one. Compared with respiration data, declining P/O ratios are an accurate indication of mitochondrial efficiency and can be used to assess the dynamic shifts of ATP within the cells.
The greater mitochondrial efficiency seen in B. medius coupled with recent work34,51; showing greater brain glycogen stores and increased O2 extractive capacity, compared with F. lapillum and F. varium, will aid greater survival at elevated temperatures in the rock pool environment but the closely matched production and hydrolysis of ATP at 30 °C will be further exacerbated as temperatures increase.
Conclusions
Assessment of the respiration rates showed declines in mitochondrial stability and function at elevated temperatures in the subtidal species, F. varium, which do not experience high temperature fluctuations. However, the higher intertidal species B. medius had greater mitochondrial efficiency and stability at elevated temperatures as was expected. These results agree with the responses from other fish species, which show decreases in mitochondrial function prior to CTmax and may be involved in setting upper thermal tolerance limits. Assessment of ATP dynamics in real-time showed that the mitochondrial capacity to produce ATP was diminished at elevated temperatures as ATP hydrolysis rates increased. This led to a closely matched supply and demand dynamic of ATP that may become further exacerbated at CTmax and could be involved in underpinning the upper thermal tolerance limit.
Data availability
The dataset supporting the results of this manuscript is available from the University of Auckland repository Research Space: https://figshare.com/s/e4d854208b6c8bcc8af7.
References
Somero, G. N. Thermal physiology and vertical zonation of intertidal animals: Optima, limits, and costs of living. Integr. Comp. Biol. 42(4), 780–789 (2002).
Hochachka, P. W. & Somero, G. N. Biochemical Adaptation: Mechanism and Process in Physiological Evolution (Oxford University Press, 2002).
Helmuth, B. et al. Living on the Edge of Two Changing Worlds: Forecasting the Responses of Rocky Intertidal Ecosystems to Climate Change Vol. 37 (ECU Publications, 2006).
Harley, C. D. et al. The impacts of climate change in coastal marine systems. Ecol. Lett. 9(2), 228–241 (2006).
Woodward, A. Climate change: Disruption, risk and opportunity. Glob. Transit. 1, 44–49 (2019).
Hoffmann, K. H. 6—Metabolic and enzyme adaptation to temperature and pressure. In The Mollusca (ed. Hochachka, P. W.) 219–255 (Academic Press, 1983).
Pörtner, H. O. & Knust, R. Climate change affects marine fishes through the oxygen limitation of thermal tolerance. Science 315(5808), 95 (2007).
Pörtner, H.-O., Bock, C. & Mark, F. C. Oxygen- and capacity-limited thermal tolerance: Bridging ecology and physiology. J. Exp. Biol. 220(15), 2685–2696 (2017).
Pörtner, H. Climate change and temperature-dependent biogeography: Oxygen limitation of thermal tolerance in animals. Naturwissenschaften 88(4), 137–146 (2001).
Verberk, W. C. et al. Does oxygen limit thermal tolerance in arthropods? A critical review of current evidence. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 192, 64–78 (2016).
Jutfelt, F. et al. Oxygen- and capacity-limited thermal tolerance: Blurring ecology and physiology. J. Exp. Biol. 221(1), jeb169615 (2018).
Ern, R. et al. Some like it hot: Thermal tolerance and oxygen supply capacity in two eurythermal crustaceans. Sci. Rep. 5, 10743 (2015).
Mitchell, P. et al. Regulation of Metabolic Processes in Mitochondria (Elsevier, 1966).
Hüttemann, M. et al. Regulation of oxidative phosphorylation, the mitochondrial membrane potential, and their role in human disease. J. Bioenerg. Biomembr. 40(5), 445 (2008).
Iftikar, F. I. & Hickey, A. J. Do mitochondria limit hot fish hearts? Understanding the role of mitochondrial function with heat stress in Notolabrus celidotus. PLoS One 8(5), e64120 (2013).
Schulte, P. M. The effects of temperature on aerobic metabolism: Towards a mechanistic understanding of the responses of ectotherms to a changing environment. J. Exp. Biol. 218(Pt 12), 1856–1866 (2015).
Power, A. et al. Uncoupling of oxidative phosphorylation and ATP synthase reversal within the hyperthermic heart. Physiol. Rep. 2(9), e12138 (2014).
Lemieux, H., Blier, P. U. & Gnaiger, E. Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: Electron flow through the Q-junction in permeabilized fibers. Sci. Rep. 7(1), 2840 (2017).
Christen, F. et al. Thermal tolerance and thermal sensitivity of heart mitochondria: Mitochondrial integrity and ROS production. Free Radic. Biol. Med. 116, 11–18 (2018).
Kiyatkin, E. A. Brain hyperthermia as physiological and pathological phenomena. Brain Res. Brain Res. Rev. 50(1), 27–56 (2005).
Kiyatkin, E. A. Brain temperature homeostasis: Physiological fluctuations and pathological shifts. Front. Biosci. (Landmark Ed) 15, 73–92 (2010).
Wang, H. et al. Brain temperature and its fundamental properties: A review for clinical neuroscientists. Front. Neurosci.-switz 8, 307–307 (2014).
Pellerin, L. & Magistretti, P. J. How to balance the brain energy budget while spending glucose differently. J. Physiol. 546(Pt 2), 325–325 (2003).
Zhao, Y. & Boulant, J. A. Temperature effects on neuronal membrane potentials and inward currents in rat hypothalamic tissue slices. J. Physiol. 564(Pt 1), 245–257 (2005).
Obel, L. F. et al. Brain glycogen-new perspectives on its metabolic function and regulation at the subcellular level. Front. Neuroenergetics 4, 3 (2012).
White, M. G. et al. Mitochondrial dysfunction induced by heat stress in cultured rat CNS neurons. J. Neurophysiol. 108(8), 2203–2214 (2012).
Walter, E. J. & Carraretto, M. The neurological and cognitive consequences of hyperthermia. Crit. Care (London, England) 20(1), 199–199 (2016).
Vornanen, M. & Paajanen, V. Seasonal changes in glycogen content and Na+-K+-ATPase activity in the brain of crucian carp. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291(5), R1482–R1489 (2006).
Hochachka, P. W. et al. Unifying theory of hypoxia tolerance: Molecular/metabolic defense and rescue mechanisms for surviving oxygen lack. Proc. Natl. Acad. Sci. U. S. A. 93(18), 9493–9498 (1996).
Chung, D. J., Bryant, H. J. & Schulte, P. M. Thermal acclimation and subspecies-specific effects on heart and brain mitochondrial performance in a eurythermal teleost (Fundulus heteroclitus). J. Exp. Biol. 220(8), 1459–1471 (2017).
Brahim, A., Mustapha, N. & Marshall, D. J. Non-reversible and reversible heat tolerance plasticity in tropical intertidal animals: Responding to habitat temperature heterogeneity. Front. Physiol. 9, 1909–1909 (2019).
Pagel, M. Inferring evolutionary processes from phylogenies. Zool. Scr. 26(4), 331–348 (1997).
Hilton, Z., Clements, K. D. & Hickey, A. J. Temperature sensitivity of cardiac mitochondria in intertidal and subtidal triplefin fishes. J. Comp. Physiol. B 180(7), 979–990 (2010).
McArley, T. J., Hickey, A. J. R. & Herbert, N. A. Hyperoxia increases maximum oxygen consumption and aerobic scope of intertidal fish facing acutely high temperatures. J. Exp. Biol. 221(22), 189993 (2018).
Gout, E. et al. Interplay of Mg2+, ADP, and ATP in the cytosol and mitochondria: Unravelling the role of Mg2+ in cell respiration. Proc. Natl. Acad. Sci. U. S. A. 111(43), E4560–E4567 (2014).
Pham, T. et al. Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. Am. J. Physiol. Cell Physiol. 307(6), C499-507 (2014).
Masson, S. W. C. et al. Mitochondrial glycerol 3-phosphate facilitates bumblebee pre-flight thermogenesis. Sci. Rep. 7(1), 13107 (2017).
Chinopoulos, C. et al. A novel kinetic assay of mitochondrial ATP-ADP exchange rate mediated by the ANT. Biophys. J. 96(6), 2490–2504 (2009).
Devaux, J. B. L. et al. Acidosis maintains the function of brain mitochondria in hypoxia-tolerant triplefin fish: A strategy to survive acute hypoxic exposure? Front. Physiol. 9, 1941 (2019).
Goo, S. et al. Multiscale measurement of cardiac energetics. Clin. Exp. Pharmacol. Physiol. 40(9), 671–681 (2013).
Lagerspetz, K. Y. Temperature effects on different organization levels in animals. Symp. Soc. Exp. Biol. 41, 429–449 (1987).
Rosenthal, J. J. & Bezanilla, F. A comparison of propagated action potentials from tropical and temperate squid axons: Different durations and conduction velocities correlate with ionic conductance levels. J. Exp. Biol. 205(Pt 12), 1819–1830 (2002).
Robertson, R. M. Thermal stress and neural function: Adaptive mechanisms in insect model systems. J. Therm. Biol. 29(7), 351–358 (2004).
Miller, N. A. & Stillman, J. H. Neural thermal performance in porcelain crabs, Genus Petrolisthes. Physiol. Biochem. Zool. 85(1), 29–39 (2012).
Gladwell, R. T., Bowler, K. & Duncan, C. J. Heat death in the crayfish Austropotamobius pallipes—Ion movements and their effects on excitable tissues during heat death. J. Therm. Biol. 1(2), 79–94 (1976).
Chen, I. & Lui, F. Neuroanatomy, Neuron Action Potential (StatPearls Publishing, 2019).
Milligan, L. P. & McBride, B. W. Energy costs of ion pumping by animal tissues. J. Nutr. 115(10), 1374–1382 (1985).
Buzatu, S. The temperature-induced changes in membrane potential. Riv. Biol. 102(2), 199–217 (2009).
Krans, J. L., Rivlin, P. K. & Hoy, R. R. Demonstrating the temperature sensitivity of synaptic transmission in a Drosophila mutant. J. Undergrad. Neurosci. Educ. 4(1), A27–A33 (2005).
Khan, J. R. et al. Thermal plasticity of skeletal muscle mitochondrial activity and whole animal respiration in a common intertidal triplefin fish, Forsterygion lapillum (Family: Tripterygiidae). J. Comp. Physiol. B 184(8), 991–1001 (2014).
McArley, T. et al. Intertidal triplefin fishes have a lower critical oxygen tension (Pcrit), higher maximal aerobic capacity, and higher tissue glycogen stores than their subtidal counterparts. J. Comp. Physiol. B. 189, 399–411 (2019).
Pfleger, J., He, M. & Abdellatif, M. Mitochondrial complex II is a source of the reserve respiratory capacity that is regulated by metabolic sensors and promotes cell survival. Cell Death Dis. 6(7), e1835–e1835 (2015).
Brand, M. D. The efficiency and plasticity of mitochondrial energy transduction. Biochem. Soc. Trans. 33(Pt 5), 897–904 (2005).
Brown, J. H. et al. Toward a metabolic theory of ecology. Ecology 85(7), 1771–1789 (2004).
Salin, K. et al. Variation in the link between oxygen consumption and ATP production, and its relevance for animal performance. Proc. Biol. Sci. 2015(282), 20151028–20151028 (1812).
Findly, R. C., Gillies, R. J. & Shulman, R. G. In vivo phosphorus-31 nuclear magnetic resonance reveals lowered ATP during heat shock of Tetrahymena. Science 219(4589), 1223 (1983).
Sharma, H. S. Neurobiology of Hyperthermia (Elsevier, 2011).
Salin, K. et al. Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol. Rep. 4(20), e13007 (2016).
Hinkle, P. C. P/O ratios of mitochondrial oxidative phosphorylation. Biochim. Biophys. Acta BBA Bioenerg. 1706(1), 1–11 (2005).
Acknowledgements
The authors would like to thank Lucy Van Oosterom and Peter Schlegel for their invaluable help with animal collection. Our thanks to members of ASML for their support and encouragement.
Funding
The work was fully supported by the Royal Society of New Zealand Marsden fund (14-UOA-210).
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Conceptualization: J.R.W., A.J.R.H., J.B.L.D.; methodology: J.R.W., A.J.R.H., J.B.L.D.; validation: J.R.W., A.J.R.H., J.B.L.D.; formal analysis: J.R.W., A.J.R.H., J.B.L.D.; investigation: J.R.W., A.J.R.H., J.B.L.D.; resources: A.J.R.H.; data curation: J.R.W., J.B.L.D.; writing—original draft: J.R.W.; writing—review & editing: J.R.W., A.J.R.H., J.B.L.D.; supervision: A.J.R.H., J.B.L.D.; project administration: A.J.R.H.; funding acquisition: A.J.R.H.
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Willis, J.R., Hickey, A.J.R. & Devaux, J.B.L. Thermally tolerant intertidal triplefin fish (Tripterygiidae) sustain ATP dynamics better than subtidal species under acute heat stress. Sci Rep 11, 11074 (2021). https://doi.org/10.1038/s41598-021-90575-y
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DOI: https://doi.org/10.1038/s41598-021-90575-y
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