Abstract
This study evaluated the protective effect of TiF4 and chitosan toothpaste on erosive tooth wear (ETW) in vitro. Enamel and dentin samples were randomly assigned to toothpastes (n = 12): (G1) TiF4 (1400 ppm F−), (G2) 0.5% chitosan (75% deacetylation, 500 mPas), (G3) TiF4 (1400 ppm F−) plus 0.5% chitosan (75% deacetylation, 500 mPas), (G4) Placebo, (G5) Erosion Protection (Elmex-GABA, 1400 ppm F−). Twelve samples were only eroded. All samples were submitted to erosive pH cycles and G1 to G5 to abrasive challenges using toothpastes’ slurries plus 45 s of treatment, for 7 days. The final profile was overlaid to the baseline one for the ETW calculation (µm). The data were subjected to Kruskal–Wallis/Dunn tests. TiF4 toothpastes, regardless of the presence of chitosan, were able to significantly reduce ETW compared to placebo, while chitosan alone was similar to placebo for both tissues. The toothpastes containing TiF4 were even superior to the commercial Elmex toothpaste on enamel, while they were similar on dentin; both were also significantly different from placebo for both tissues. TiF4 and Elmex toothpastes minimized the impact of brushing on eroded surface. In conclusion, TiF4 toothpastes, regardless the presence of chitosan, showed to be effective in minimizing ETW in vitro.
Similar content being viewed by others
Introduction
Erosive tooth wear (ETW) is the cumulative superficial loss of mineralized tooth substance due to chemical (erosion) and physical process (attrition, abrasion), in which erosion is the predominant etiological factor, associated with mechanical action of toothbrushing, for example1,2. The dentition naturally undergoes some wear overtime; however, the rate of wear should be extremely slow to maintain healthy the tooth morphology and functions throughout the lifetime3. Tooth wear can be defined as pathological if it is beyond the physiological level relative to the individual’s age, depending on its severity (dentin exposure) and if it interferes with the self-perception of well-being due to the presence of pain, function and/or aesthetics compromises2,4.
The increase in the prevalence and clinical detection of ETW in recent decades has attracted the attention of the dental community around the world5,6,7. The older age groups generally show high levels of wear8 and severe ETW may have impact on the quality of life of the affected individuals9.
Among the various strategies to control ETW, the most tested one is the application of fluorides10,11,12 especially those containing Sn2+ or Ti4+, polyvalent metals that interact with the tooth structure, forming a more acidic resistant layer compared to CaF2 induced by the application of conventional fluorides as NaF11,13. The daily application of a solution containing TiF4/NaF has promising results compared to those obtained with the commercial fluoridated solution (SnCl2 and NaF/AmF), clinically indicated for ETW, under in vitro and in situ models13,14,15. It has also shown to be more promising than a unique professional application of TiF4 varnish16. TiF4 incorporated into a toothpaste has also shown to reduce the ETW created by the association of erosive and abrasive challenges17.
Another compound of interest to control ETW is chitosan. Chitosan is a natural polymer derived from chitin deacetylation that has the ability to interact electrostatically with the tooth structure and easily adsorbs to enamel forming a protective layer18. Toothpaste containing chitosan (Chitodent®—Helmuth Focken Biotechnik) is able to inhibit ETW, showing similarity to that containing NaF; however, its protective effect is reduced when brushing forces are applied19,20.
To overcome this issue, chitosan has been added to fluoridated toothpastes21. Fluoridated toothpastes containing tin (around 3500 ppm Sn2+ and 1400 ppm F−), in the presence of chitosan (0.5%), have a better protective effect against erosive enamel wear than those with fluoride only19,21. Chitosan can increase the retention of tin to enamel, which may, at least in part, explain its protective effect in association with F− and Sn2+22.
Taking this idea in mind, it is expected improvement of the protective effect of TiF4 toothpaste, already tested17, with the inclusion of chitosan into the formulae. Recently, we have shown that solutions containing TiF4/NaF and chitosan had a protective effect against enamel wear similar to the positive control (Elmex® GABA solution, containing Sn2+ and F−)23, while for dentin, no improvement in the effect of the fluoridated solution was seen with the addition of chitosan24.
Therefore, the aim of this work was to evaluate the protective effect of TiF4 and chitosan containing toothpaste on ETW on both enamel and dentin, in vitro. The null hypothesis is that no difference exists between TiF4 toothpaste, with or without chitosan, with respect to the protective effect on ETW.
Materials and methods
Sample preparation
The study was approved by the Local Ethics Committee for Animal Use (number 007/2020). Besides, all methods were performed in accordance with the relevant guidelines (ARRIVE guidelines) and regulations.
Seventy-two bovine enamel and 72 root dentin samples were prepared from incisors stored in 0.1% thymol solution (pH 7.0). The roots were separated from the crowns and both were then separately coupled to a prefabricated silicone mold (Biopdi, São Carlos, Brazil) and embedded in autopolymerizing acrylic resin, allowing the exposition of the labial surface. The samples were polished using silicon carbide sandpapers (320, 600 and 1200 grades of Al2O3 papers; Buehler, Lake Bluff, USA). Afterwards, the baseline profile was measured by using a contact profilometer and two thirds of the samples’ surfaces were protected with red nail polish (Risqué®, São Paulo, Brazil), to obtain two control areas13,14.
The samples were randomly assigned to 5 toothpastes (n = 12): (G1) experimental containing TiF4; (G2) chitosan; (G3) TiF4 plus chitosan; (G4) placebo (no F and no chitosan), (G5) commercial Erosion Protection (Elmex®—GABA, Switzerland). Twelve samples were only eroded (control). Table 1 shows the details about the toothpastes.
The chitosan toothpaste was prepared as described for the experimental solution23,24. All toothpastes were diluted (1 part toothpaste to 3 parts deionized water by weight; hereafter named slurry) for the treatment. The pH of the slurries was measured in duplicate using a pH meter previously calibrated to pH 4.1 and 7.0 standards (Orion 3-star pH Bench Top, Thermo Electron Corporation, USA). The toothpastes were applied at their natural pH, since TiF4 is less effective in preventing tooth erosion when its pH is buffered to a high value25.
Erosive and abrasive cycling
The samples were subjected to daily erosive and abrasive challenges for 7 days26,27. Erosion was induced 4 times a day by using 0.1% citric acid solution (pH 2.5) for 90 s (30 mL/sample) at 25 °C. The samples were then washed in deionized water (5 s) and immersed in artificial saliva28 (pH 6.8, 30 mL/sample) for 2 h between the erosive challenges at 25 °C.
After the first and the last daily erosive challenges, the samples from G1 to G5 were subjected to abrasion for 15 s (except the erosion only—G6), using toothbrushing machine (Biopdi®, São Carlos, Brazil), toothbrush (5460 ultrasoft Curaprox®, Kriens, Switzerland, 1 toothbrush/sample) and the toothpastes’ slurries (1:3 water, 15 mL/sample, 37 °C) under standardized velocity (3 linear movements/s) and force (1.5 N)26,27. Afterwards, the samples were kept for further 45 s in contact with the toothpastes’ slurries to complete 1 min of treatment and washed using deionized water for 10 s. The samples were kept in artificial saliva overnight completing 24 h of cycling.
After 7 days, the nail polish was removed by using acetone solution and the ETW (final profile) was measured.
Contact profilometry
ETW was determined using a contact profilometer (Mahr Perthometer, Göttingen, Germany). Five equidistant surface scans of each sample were performed (4.12 ± 0.59 mm for enamel and 6.22 ± 1.20 mm for dentin, 250 μm apart from each other) at the baseline and at the final measurement. To achieve the repeatability, the samples presented an identification mark (small drillings made with drill ¼, Jet Carbide, Kerr, Joinvile, Brazil) and two scratches to delimitate the exposed area. They were inserted into a metal device (x and y axes determination, reproducibility of 0.08 μm), to allow the stylus to be accurately repositioned at each measurement. The baseline profile was compared to the final profile for the calculation of the ETW by using the software Marh Surf XCR20. This analysis was done under 100% humidity for dentin13,14,23,24.
The scans were superposed (final profiles versus baseline profile) and the average depth of the under-curve area was calculated, considering the limit of detection of the system of 0.5 μm13,14,23,24.
Statistical analysis
The ETW data were statistically compared using Kruskal–Wallis followed by Dunn test, since no equality of variances was found (Bartlett’s test), for both tissues separately. The software applied was Graph Pad Instat (San Diego, USA) and the level of significance was set at 5%13,14,23,24.
Ethics declarations
The study was approved by the Ethics Committee for Animal Use of Bauru School of Dentistry—USP (Number 007/2020). It follows the recommendations in the ARRIVE guidelines. All methods were performed in accordance with the relevant guidelines and regulations.
Results
The experimental TiF4 toothpastes, regardless of the presence of chitosan, were able to significantly reduce enamel and dentin wear (about 80% of prevention) compared to placebo, while chitosan alone was similar to placebo for both tissues. The toothpastes containing TiF4 were even superior to the commercial Elmex® toothpaste in reducing enamel wear, but they were similar in case of dentin, and both significantly reduced tooth wear compared to placebo (p < 0.0001). Both TiF4 and Elmex® toothpastes were not significantly different compared to the erosion condition for enamel and presented lower values compared to erosion for dentin, which means that they significantly reduced the abrasive brushing effect on eroded tooth surface (Figs. 1, 2).
Discussion
The null hypothesis of this work was accepted, since the inclusion of chitosan did not improve the protective effect of TiF4 toothpaste, which in fact was even better than the positive control at least for reducing enamel wear. The experimental toothpastes containing TiF4 and chitosan demonstrated a protective effect of 89% for enamel and 78% for dentin compared to placebo, whereas the commercial Elmex® presented 43% and 71% of protective fraction, respectively.
According to previous studies, the protective capacity of TiF4 on the enamel is justified by the action not only of fluoride, but also of titanium29,30. TiF4 has been added to several formulations (such as varnish and solution), proving to be an effective compound against tooth demineralization (both caries and dental erosion) when compared to formulations containing NaF in vitro and in situ13,15,30,31,32 Titanium minimizes tooth demineralization, since it tends to complex with apatite, forming a “glaze” layer rich in titanium oxide and hydrated titanium phosphate, which is more acid-resistant than the layer of CaF2 induced by the application of NaF30. In addition, TiF4 induces greater CaF2 precipitation than NaF due to its low pH30.
Chitosan was added into the toothpaste containing TiF4, to improve its protective effect, since the literature has shown that the addition of chitosan to fluoridated solutions and toothpastes leads to a reduction in ETW23,24,33,34. A recent work has shown benefit of the association between TiF4/NaF and 0.5% chitosan on the protection of enamel wear in vitro24. This biopolymer is able to adsorb to enamel, creating a positively charged and more hydrophobic surface20,35, providing a mechanical barrier against acids20. This mechanism justifies its role as a mechanical barrier against the penetration of acids, contributing to the inhibition of demineralization36. However, when applied isolated (without fluoride), its protective effect is reduced by brushing forces, as shown by our study and others19,20. Our study also showed that the addition of chitosan to TiF4 toothpaste did not improve the protection, regardless the tooth substrate, indicating that chitosan, under the tested conditions, may not interact with the tooth surface producing an organic layer as expected.
An interesting result found in this study is that the experimental TiF4 toothpaste was superior to the commercial version indicated for controlling ETW in case of enamel. The commercial Elmex® Erosion Protection toothpaste has in its formulation: F (as AmF and NaF, 1400 ppm), Sn2+ (as SnCl2, 3500 ppm), and chitosan (0.5%)21,22,37. Ganss et al.22 observed, in an in vitro study, applying more frequent erosive challenges and a longer treatment time with toothpaste, 68% reduction in enamel wear with the use of Elmex® Erosion Protection toothpaste compared to placebo. Schlueter et al.21,37, using similar methodology, but in situ, reported a reduction of approximately 50% of enamel wear by the use of Elmex® Erosion Protection compared to placebo, which is in agreement with our work.
Tin, like titanium, has an interaction with the tooth structure, being incorporated into the tooth surface and creating a mechanical barrier together with fluoride precipitates. When tin is combined with chitosan, there is a synergistic effect due to the formation of tightly connected multilayers12,22,37 acting as a shield for the deposition of Sn2+ and increasing the preventive effect of this complex structure against ETW. This scenario was not observed in the case of TiF4.
Differently from enamel, studies involving dentin are scarce. Elmex® Erosion Protection toothpaste reduces dentine wear, but not at superior level compared to a conventional fluoride toothpaste38. Tin may react with the dentin surface regardless of the presence of the demineralized collagen layer. In cases in which the organic matrix is preserved, phosphoproteins might attract the tin ion, which is then retained in the organic matrix to some extent but also accumulates in the underlying mineralized tissue. Under the absence of the demineralized organic matrix layer, the reaction is by precipitation39.
One factor that could have influenced the lack of synergic effect of chitosan and TiF4 is the erosive challenge, since the benefit of metal fluorides has been more evident when erosive challenges are longer12,37. Another important aspect is that the presence of abrasive silica in toothpaste can have limited the protective effect of chitosan associated with TiF4, when compared to fluoridated gels that do not have abrasive that could interact with chitosan20,40. It is also relevant to discuss that the effect of chitosan is also dependent of the low pH of the vehicle. In case of our study, the final pH value of toothpaste containing chitosan alone was close to neutral, which can reduce the protonation of the molecule and its protective effect, which should be considered. Previous works testing solution containing chitosan, at low pH, showed better effect on ETW23,24 than the tested toothpaste.
It is known that abrasive wear of eroded hard tissues is considered an adverse side effect of aggressive tooth brushing, which is determined mainly by the abrasiveness of toothpaste rather than by the toothbrush41,42. Based on this, the beneficial effect of TiF4 toothpaste, regardless of chitosan, may be not only due to the fluoride and titanium, but also to its low abrasivity. Thus, the need for further studies in the area is irrefutable, to analyze RDA/REA values of the experimental toothpastes and the effect of aggressive erosive challenges on their protection capacity. Other important point for the future is to buffer the toothpastes in order to have similar final pH (around 4.5) for all.
A limitation of the present study was the absence of human saliva, which is justified by the difficult to collect the amount needed for a pH cycling model of 7 days. Chitosan seems to have a great affinity to salivary proteins, reacting better with the tooth surface in the presence of those proteins40,43,44. Despite Luka et al.45 showed no improvement of the protective effect of Sn2+/F−/chitosan toothpastes under the presence of mucin in vitro, the present result shall be confirmed under in situ model, a condition closer to in vivo situation, with the presence of human saliva that may interplay the action of active compounds on the tooth46,47. Although chitosan did not have any protective effect when included into TiF4 toothpaste, it increased the toothpaste pH closer to the pH value of the commercial toothpaste, which is more suitable for a daily use.
Conclusion
Based on the results, we conclude that TiF4 toothpastes, regardless of the presence of chitosan, are effective in minimizing tooth wear caused by brushing of eroded surface, which shall be confirmed under clinical studies. Although the chitosan was not able to improve the protective effect of the TiF4 toothpaste under this model, it increased the pH of the toothpaste to a more acceptable value for home-use oral care products.
Data availability
All data generated or analyzed during this study are included in this article.
References
Carvalho, T. S. & Lussi, A. Chapter 9: Acidic beverages and foods associated with dental erosion and erosive tooth wear. Monogr. Oral Sci. 28, 91–98. https://doi.org/10.1159/000455376 (2020).
Schlueter, N. et al. Terminology of erosive tooth wear: Consensus Report of a Workshop Organized by the ORCA and the Cariology Research Group of the IADR. Caries Res. 54, 2–6. https://doi.org/10.1159/000503308 (2020).
Lussi, A. et al. The use of fluoride for the prevention of dental erosion and erosive tooth wear in children and adolescents. Eur. Acad. Paediat. Dent. 20, 517–527. https://doi.org/10.1007/s40368-019-00420-0 (2019).
Wetselaar, P., Wetselaar-Glas, M., Katzer, L. D. & Ahlers, M. O. Diagnosing tooth wear, a new taxonomy based on the revised version of the tooth wear evaluation system (TWES 2.0). J. Oral Rehabil. 47, 703–712. https://doi.org/10.1111/joor.12972 (2020).
Hasselkvist, A. & Arnrup, K. Prevalence and progression of erosive tooth wear among children and adolescents in a Swedish county, as diagnosed by general practitioners during routine dental practice. Heliyon 11, e07977. https://doi.org/10.1016/j.heliyon.2021.e07977 (2021).
Donovan, T., Nguyen-Ngoc, C., Alraheam, I. A. & Irusa, K. Contemporary diagnosis and management of dental erosion. J. Esthet. Restor. Dent. 33, 78–87. https://doi.org/10.1111/jerd.12706 (2021).
Martignon, S. et al. Epidemiology of erosive tooth wear, dental fluorosis and molar incisor hypomineralization in the American continent. Caries Res. 55, 1–11. https://doi.org/10.1159/000512483 (2021).
Bartlett, D. & O’Toole, S. Tooth wear and aging. Aust. Dent. J. 64, S59–S62. https://doi.org/10.1111/adj.12681 (2019).
Mehta, S. B., Loomans, B. A. C., Banerji, S., Bronkhorst, E. M. & Bartlett, D. An investigation into the impact of tooth wear on the oral health related quality of life amongst adult dental patients in the United Kingdom, Malta and Australia. J. Dent. 99, 103409. https://doi.org/10.1016/j.jdent.2020.103409 (2020).
Magalhães, A. C., Wiegand, A., Rios, D., Buzalaf, M. & Lussi, A. Fluoride in dental erosion. Monogr. Oral Sci. 22, 158–170. https://doi.org/10.1159/000325167 (2011).
Pini, N. I. P., Lima, D. A. N. L., Luka, B., Ganss, C. & Schlueter, N. Viscosity of chitosan impacts the efficacy of F/Sn containing toothpastes against erosive/abrasive wear in enamel. J. Dent. 92, 103247. https://doi.org/10.1016/j.jdent.2019.103247 (2020).
Fiorillo, L., Cervino, G., Herford, A. S., Laino, L. & Cicciù, M. Stannous fluoride effects on enamel: A systematic review. Biomimetics (Basel) 5, 41. https://doi.org/10.3390/biomimetics5030041 (2020).
Souza, B. M., Lima, L. L., Comar, L. P., Buzalaf, M. A. & Magalhães, A. C. Effect of experimental mouthrinses containing the combination of NaF and TiF4 on enamel erosive wear in vitro. Arch. Oral Biol. 59, 621–624. https://doi.org/10.1159/000479038 (2014).
Castilho, A. R., Salomão, P. M., Buzalaf, M. A. & Magalhães, A. C. Protective effect of experimental mouthrinses containing NaF and TiF4 on dentin erosive loss in vitro. J. Appl. Oral Sci. 23, 486–490. https://doi.org/10.1590/1678-775720150127 (2015).
de Souza, B. M., Santi, L. R. P., Silva, M. S., Buzalaf, M. A. R. & Magalhães, A. C. Effect of an experimental mouthrinse containing NaF and TiF4 on tooth erosion and abrasion in situ. J. Dent. 73, 45–49. https://doi.org/10.1016/j.jdent.2018.04.001 (2018).
Magalhães, A. C. et al. Effect of a single application of TiF4 varnish versus daily use of a low-concentrated TiF4/NaF solution on tooth erosion prevention in vitro. Caries Res. 50, 462–470. https://doi.org/10.1159/000448146 (2016).
Comar, L. P. et al. Effect of NaF, SnF(2), and TiF(4) toothpastes on bovine enamel and dentin erosion-abrasion in vitro. Int. J. Dent. 2012, 134350. https://doi.org/10.1155/2012/134350 (2012).
Kumar, D., Gihar, S., Shrivash, M. K., Kumar, P. & Kundu, P. P. A review on the synthesis of graft copolymers of chitosan and their potential applications. Int. J. Biol. Macromol. 163, 2097–2112. https://doi.org/10.1016/j.ijbiomac.2020.09.060 (2020).
Ganss, C., Lussi, A., Grunau, O., Klimek, J. & Schlueter, N. Conventional and anti-erosion fluoride toothpastes: Effect on enamel erosion and erosion-abrasion. Caries Res. 45, 581–589. https://doi.org/10.1159/000334318 (2011).
Ganss, C., Marten, J., Hara, A. T. & Schlueter, N. Toothpastes and enamel erosion/abrasion—Impact of active ingredients and the particulate fraction. J. Dent. 54, 62–67. https://doi.org/10.1016/j.jdent.2016.09.005 (2016).
Schlueter, N., Klimek, J. & Ganss, C. Randomised in situ study on the efficacy of a tin/chitosan toothpaste on erosive-abrasive enamel loss. Caries Res. 47, 574–581. https://doi.org/10.1159/000351654 (2013).
Ganss, C. et al. Efficacy of the stannous ion and a biopolymer in toothpastes on enamel erosion/abrasion. J. Dent. 40, 1036–1043. https://doi.org/10.1016/j.jdent.2012.08.005 (2012).
Souza, B. M., Machado, P. F., Vecchia, L. R. & Magalhães, A. C. Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro. Eur. J. Oral Sci. 128, 495–500. https://doi.org/10.1111/eos.12740 (2020).
de Souza, B. M., Santi, L. R. P., João-Souza, S. H., Carvalho, T. S. & Magalhães, A. C. Effect of titanium tetrafluoride/sodium fluoride solutions containing chitosan at different viscosities on the protection of enamel erosion in vitro. Arch. Oral Biol. 120, 10492. https://doi.org/10.1016/j.archoralbio.2020.104921 (2020).
Wiegand, A. et al. Effect of TiF4, ZrF4, HfF4 and AmF on erosion and erosion/abrasion of enamel and dentin in situ. Arch. Oral Biol. 55, 223–228. https://doi.org/10.1016/j.archoralbio.2009.11.007 (2010).
Mosquim, V., Souza, B. M., Foratori, J. G. A., Wang, L. & Magalhães, A. C. The abrasive effect of commercial whitening toothpastes on eroded enamel. Am. J. Dent. 30, 142–146 (2017).
Vertuan, M., de Souza, B. M., Machado, P. F., Mosquim, V. & Magalhães, A. C. The effect of commercial whitening toothpastes on erosive dentin wear in vitro. Arch. Oral Biol. 109, 104580. https://doi.org/10.1016/j.archoralbio.2019.104580 (2020).
Klimek, J., Hellwig, E. & Ahrens, G. Fluoride taken up by plaque, by the underlying enamel and by clean enamel from three fluoride compounds in vitro. Caries Res. 16, 156–161. https://doi.org/10.1159/000260592 (1982).
Tezel, H., Ergücü, Z. & Onal, B. Effects of topical fluoride agents on artificial enamel lesion formation in vitro. Quintessence Int. 33, 347–352 (2002).
Comar, L. P. et al. Mechanism of action of TiF4 on dental enamel surface: SEM/EDX, KOH-soluble F, and X-ray diffraction analysis. Caries Res. 51, 554–567. https://doi.org/10.1159/000479038 (2018).
Levy, F. M., Rios, D., Buzalaf, M. & Magalhães, A. C. Efficacy of TiF4 and NaF varnish and solution: A randomized in situ study on enamel erosive-abrasive wear. Clin. Oral Investig. 18, 1097–1102. https://doi.org/10.1007/s00784-013-1096-y (2014).
Comar, L. P. et al. TiF4 and NaF varnishes as anti-erosive agents on enamel and dentin erosion progression in vitro. J. Appl. Oral Sci. 23, 14–18. https://doi.org/10.1590/1678-775720140124 (2015).
Pini, N. I., Lima, D. A., Lovadino, J. R., Ganss, C. & Schlueter, N. In vitro efficacy of experimental chitosan-containing solutions as anti-erosive agents in enamel. Caries Res. 50, 337–345. https://doi.org/10.1159/000445758 (2016).
Sakae, L. O. et al. An in vitro study on the influence of viscosity and frequency of application of fluoride/tin solutions on the progression of erosion of bovine enamel. Arch. Oral Biol. 89, 26–30. https://doi.org/10.1016/j.archoralbio.2018.01.017 (2018).
Busscher, H. J., Engels, E., Dijkstra, R. J. & van der Mei, H. C. Influence of a chitosan on oral bacterial adhesion and growth in vitro. Eur. J. Oral Sci. 116, 493–495. https://doi.org/10.1111/j.1600-0722.2008.00568.x (2008).
Arnaud, T. M., de Barros, N. B. & Diniz, F. B. Chitosan effect on dental enamel de-remineralization: An in vitro evaluation. J. Dent. 38, 848–852. https://doi.org/10.1016/j.jdent.2010.06.004 (2010).
Schlueter, N., Klimek, J. & Ganss, C. Effect of a chitosan additive to a Sn2+-containing toothpaste on its anti-erosive/anti-abrasive efficacy—A controlled randomised in situ trial. Clin. Oral Investig. 18, 107–115. https://doi.org/10.1007/s00784-013-0941-3 (2014).
Aykut-Yetkiner, A., Attin, T. & Wiegand, A. Prevention of dentine erosion by brushing with anti-erosive toothpastes. J. Dent. 42, 856–861. https://doi.org/10.1016/j.jdent.2014.03.011 (2014).
Ganss, C. et al. Mechanism of action of tin-containing fluoride solutions as anti-erosive agents in dentine—An in vitro tin-uptake, tissue loss, and scanning electron microscopy study. Eur. J. Oral Sci. 118, 376–384. https://doi.org/10.1111/j.1600-0722.2010.00742.x (2010).
Wasser, G., João-Souza, S. H., Lussi, A. & Carvalho, T. S. Erosion-protecting effect of oral-care products available on the Swiss market. A pilot study. Swiss Dent. J. 128, 290–296 (2018).
Wiegand, A., Kuhn, M., Sener, B., Roos, M. & Attin, T. Abrasion of eroded dentin caused by toothpaste slurries of different abrasivity and toothbrushes of different filament diameter. J. Dent. 37, 480–484. https://doi.org/10.1016/j.jdent.2009.03.005 (2009).
Wiegand, A. et al. Impact of toothpaste slurry abrasivity and toothbrush filament stiffness on abrasion of eroded enamel—An in vitro study. Acta Odontol. Scand. 66, 231–235. https://doi.org/10.1080/00016350802195041 (2008).
Machado, A. C. et al. Using fluoride mouthrinses before or after toothbrushing: Effect on erosive tooth wear. Arch. Oral Biol. 108, 104520. https://doi.org/10.1016/j.archoralbio.2019.104520 (2019).
Machado, A. et al. Anti-erosive effect of rinsing before or after toothbrushing with a fluoride/stannous ions solution: An in situ investigation: Application order of fluoride/tin products for erosive tooth wear. J. Dent. 101, 103450. https://doi.org/10.1016/j.jdent.2020.103450 (2020).
Luka, B., Arbter, V., Sander, S., Duerrschnabel, K. A. & Schlueter, N. Impact of mucin on the anti-erosive/anti-abrasive efficacy of chitosan and/or F/Sn in enamel in vitro. Sci. Rep. 11, 5285. https://doi.org/10.1038/s41598-021-84791-9 (2021).
Buzalaf, M. A., Hannas, A. R. & Kato, M. T. Saliva and dental erosion. J. Appl. Oral Sci. 20, 493–502. https://doi.org/10.1590/s1678-77572012000500001 (2012).
Kensche, A. et al. Effect of fluoride mouthrinses and stannous ions on the erosion protective properties of the in situ pellicle. Sci. Rep. 9, 5336. https://doi.org/10.1038/s41598-019-41736-7 (2019).
Acknowledgements
We thank FAPESP for the concession of a scholarship and a grant to the second and to the last authors, respectively (Proc. 2018/26369-4; 2019/21797-0). This publication is a thesis submitted by the first author to Bauru School of Dentistry, University of São Paulo, in fulfilment of the requirements for a MS degree in Oral Biology.
Author information
Authors and Affiliations
Contributions
M.F. contributed to Methodology; Validation; Formal analyses; Investigation; Data curation; Writing-Original Draft; Writing-Review & Editing; Visualization; I.G. contributed to Investigation and Funding acquisition; M.V. contributed to Investigation; B.d.S. contributed to Conceptualization; Methodology and Investigation; and A.M. contributed to Conceptualization; Methodology; Validation; Formal analyses; Resources; Data curation; Writing-Original Draft; Writing-Review & Editing; Visualization; Supervision; Project administration and Funding acquisition. All authors: final approval of the version to be published.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Francese, M.M., Gonçalves, I.V.B., Vertuan, M. et al. The protective effect of the experimental TiF4 and chitosan toothpaste on erosive tooth wear in vitro. Sci Rep 12, 7088 (2022). https://doi.org/10.1038/s41598-022-11261-1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-022-11261-1
- Springer Nature Limited