Abstract
Despite numerous reports on the altered sphingolipids metabolism in human cancers, their clinical significance in breast cancer remains obscure. Previously, we identified the high levels of sphingolipids, ceramide phosphates and sphingosine phosphates, and the genes involved in their synthesis, CERK and SPHK1, in breast cancer patients. The present study aimed to determine the correlations of CERK and SPHK1 with clinical outcomes as well as metastasis and drug resistance markers. Both local and TCGA cohorts were analysed. High-confidence regulatory interaction network was constructed to find association of target genes with metastasis and drug resistance. Furthermore, correlations of CERK and SPHK1 with selected metastasis and drug resistance markers were validated in both cohorts. Overexpression of CERK and SPHK1 was associated with nodal metastasis, late tumor stage and high proliferation potency. In addition, increased CERK expression was also indicative of poor patient survival. Computational network analysis revealed the association of CERK and SPHK1 with known metastasis markers MMP-2 and MMP-9 and drug resistance markers ABCC1 and ABCG2. Correlation analysis confirmed the associations of target genes with these markers in both local as well as TCGA cohort. The above findings suggest clinical utility of CERK and SPHK1 as potential biomarkers in breast cancer patients and thus could provide novel leads in the development of therapeutics.
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Introduction
Breast cancer is the most common neoplasm in women and is the leading cause of morbidity and mortality worldwide1. In 2020, approximately 22,61,419 new cases of breast cancer were reported globally2. Studies found that the chances of a woman to be diagnosed with breast cancer during her lifetime is approximately 1 in 8 women (12.5%) while the chances of dying from the disease is 1 in 39 women (2.5%)3. Accumulating evidence suggest that early-stage screening and detection of the cancer can lead to improved diagnosis and prognosis and reduce the associated mortality. Despite the availability of various screening and treatment modalities, the incidence rate of breast cancer is increasing at an alarming rate. To overcome the existing lacunae, current studies are aligned towards the development of reliable biomarkers that can quantify the risk of patients and favour a better therapeutic approach early in the course of treatment.
A major clinical challenge associated with successful breast cancer therapy is metastasis. Metastatic tumors in most cases are reported to be resistant to cytotoxic drugs and other conventional treatments. Distant metastatic breast cancer (MBC) is the most severe form of breast cancer and the five-year survival rate of MBC is only 27%3. Tumor cells interact with their microenvironment and elicit the expression of growth factors, chemokines, matrix metalloproteinases (MMPs) which lead to metastasis by inducing the process of epithelial mesenchymal transition (EMT)4. In addition to metastasis, multidrug resistance (MDR) in tumor cells is another crucial obstacle that impairs the efficacy of both conventional and novel molecular therapies. The principal molecular mechanism involved in drug resistance is the increased rate of drug efflux by the members of the ATP-Binding Cassette (ABC) transporter superfamily such as ABCB1, ABCC1 and ABCG25. Overexpression of these drug transporters has been found to be associated with MDR in prostate, small cell lung carcinoma and breast cancer6. In addition to their role in drug efflux, ABC transporters also mediate the movement of lipids and other metabolic products across the plasma and intracellular membranes.
Sphingolipids are an important class of lipids that play key cellular roles both as structural components of membranes as well as signaling molecules. The metabolism of sphingolipids generates bioactive metabolites that participate in the regulation of cancer development, proliferation, progression, metastasis and chemoresistance in various cancer types7. The sphingolipid metabolite, sphingosine-1-phosphate (S1P) is known to contribute to EMT pathway by modulating the levels of MMPs such as MMP-2 and MMP-98. Increased expression of SPHK1 has also been shown to promote colon cancer growth and progression by inducing the expression of MMP-2 and MMP-99,10. Numerous studies have linked the development of drug resistance to changes in the sphingolipid metabolism11,12,13.
Our group has previously identified sphingolipid metabolites ceramide-1-phosphate (CerP), S1P and sphingomyelins (SM) to be differentially regulated between tumor and adjacent normal breast tissues and their clinical relevance as biomarkers was determined14. Consistent with the above results, an upregulation in the level of genes, ceramide kinase (CERK), sphingosine kinase 1 (SPHK1) and sphingomyelin synthase 1 (SGMS1), involved in the synthesis of these metabolites was also observed in local and The Cancer Genome Atlas (TCGA) cohort. Of the abovementioned genes, CERK and SPHK1 had a significant potential to discriminate between tumor and adjacent normal breast tissues. However, the clinical utility of these genes and their association with cancer cellular processes in breast cancer is still not explored and needs further understanding.
In the present study, we analysed the clinicopathological correlations of CERK and SPHK1, and their ability to predict patient outcome in various clinical sub-groups. Furthermore, interaction network-based analyses were performed (by integrating gene co-expression regulations and physical PPIs) to determine the functional relatedness of these genes with metastasis and drug resistance in breast cancer. The findings were then validated in local as well as TCGA cohort.
Results
The clinicopathological characteristics of the patients were described in our previous study14.
Histopathological examinations of breast tissue
Morphological assessment of breast biopsy tissue was carried out on routine hematoxylin & eosin (H & E) -stained paraffin sections. Normal breast epithelial cells in the terminal duct lobular units had uniform shapes and sizes having uniform monomorphic nuclei with finely dispersed chromatin (Fig. 1A and B). Tumour cells were arranged in tubules, clusters, cords and trabeculae. Tumour cell with grade I histology showed mildly enlarged nuclei which are fairly uniform with visible nucleoli, mitosis was infrequent (Fig. 1C). Grade II tumour cells showed moderate degree nuclear pleomorphism, moderately enlarged and occasionally showing tubular differentiation. Mitosis was occasionally visible (Fig. 1D). Tumour cells in a high grade or grade III carcinoma showed gross nuclear pleomorphism arranged in sheets, large clusters and trabeculae, no tubular differentiation was visible. Individual cells showed marked nuclear pleomorphism, irregularly clumped chromatin, prominent nucleoli and frequent mitosis. (Fig. 1E).
Clinical Relevance of CERK and SPHK1
Correlation of CERK and SPHK1 with clinicopathological characteristics
We previously reported an overexpression of CERK and SPHK1 in breast tumor tissues as compared to adjacent normal tissues14. To further strengthen the clinical relevance of these genes in breast cancer patients, their correlations with age, ER status, tumor grade, nodal status, tumor stage and proliferation marker were evaluated. There was no significant difference observed in the expression of these genes according to age and tumor grades of patients. Significant high level of CERK was observed in ER Positive (*p = 0.049) (Fig. 2A), nodal positive (*p = 0.047) (Fig. 2B) and late-stage breast cancer patients (*p = 0.037) (Fig. 2C). A significant positive correlation was also observed between the levels of CERK and proliferation marker Ki67 (*p = 0.0347) (Fig. 2 D). Similar results were observed for SPHK1, with high levels in nodal positive patients (*p = 0.0427) (Fig. 3A), late-stage patients (**p = 0.008) (Fig. 3B), and a positive association with Ki67 (*p = 0.0425) (Fig. 3C). The expression of SPHK1 was not observed to be corelated with the ER status of the patient. In TCGA cohort, no significant correlation of CERK and SPHK1 with clinicopathological characteristics was observed.
Protein expression of CERK and SPHK1 in breast cancer patients
To further validate alterations in the levels of CERK and SPHK1, the levels of corresponding proteins were also analysed in local cohort samples using western blotting (Fig. 4A) (Supplementary Figs. 1 and 4). In the present study, protein expression of both SPHK1 (*p = 0.041) (Fig. 4 B) and CERK (**p = 0.0028) (Fig. 4C) were found to be significantly upregulated in tumor tissue as compared to adjacent normal tissue.
Correlation of CERK and SPHK1 with overall survival (OS) in breast cancer
To test the prognostic values of CERK and SPHK1, their association with OS was studied in breast cancer patients using Kaplan–Meier plot. A total of 526 breast cancer patients from TCGA dataset were divided into low expression and high expression group based on the median expression. There was no association found between the expression of CERK and OS (Fig. 5A). Similarly, SPHK1 was also not found to correlate with the OS of breast cancer patients. To further understand the relationship between the genes and survival, the patients were divided into various subgroups. The expression of SPHK1 was observed to have no impact on OS among various breast cancer subgroups. However, high CERK expression was related to poor OS in patients with lymph node metastasis (*p = 0.023) (Fig. 5C) and late-stage breast cancer patients (*p = 0.040) (Fig. 5E). No other associations were observed between CERK expression and other clinical subgroups (Fig. 5B and D).
Relationship of CERK and SPHK1 with metastasis and drug resistance
Network inference and its characteristics
To obtain an understanding of regulatory interactions between CERK, SPHK1, and metastasis and drug resistance markers, the underlying protein–protein interaction (PPI) network was inferred. The PPI network comprised 18,066 interactions among 506 proteins, including candidate proteins (CERK and SPHK1) along with metastasis markers, MMP-2 and MMP-9, and drug resistance markers, ABCC1 and ABCG2. To affirm the biological significance of network, the degree distribution of established PPI network was computed and compared to randomly generated networks. The PPI network was highly skewed and the node degree distribution approximated the power-law distribution (Fig. 6A), indicating the biological significance of network and presence of some exceptionally connected proteins. In this aspect, topological analyses of the network were performed to assess the centrality of candidate proteins. Centrality measure k specifies the number of interactions a protein has within the interaction network and the degree distribution predict the highly connected proteins called hubs15,16. The other centrality measure betweenness centrality (BC) measures the information flow across the network, and predicts non-hubs that play roles of bottlenecks in the network17. Both k and BC centrality measures are well-known to be important in selecting globally and locally important proteins respectively, in the network18 and were therefore considered for the network analysis after assessing their biological relevance (Supplementary Data R1). Metastasis markers MMP-2 and MMP-9 were among the top-hub as well as among the top-bottlenecks whereas ABCC1 and ABCG2 were only among the top-bottleneck drug resistance proteins. While ABCC1 and ABCG2 drug transporters were the primary interactors of target proteins, another transporter, Spinster homolog 2 (SPNS2) was also observed in the network as a transitive protein between CERK and ABCG2. CERK was neither a network hub nor a bottleneck. While SPHK1 was not a network hub, it was among the top-bottleneck proteins indicating it be a highly ‘between’ among small network clusters and thus could possibly play important roles in mediating signaling during cancer progression.
To explore the network-based correlations among CERK and SPHK1, MMP-2 and MMP-9, and ABCC1 and ABCG2, a sub-network comprising interactions among candidates was extracted (Fig. 6B) from the large PPI network. In biological aspects, shortest path lengths compute the ‘functional closeness’ of proteins in a biological network. CERK and SPHK1 had smaller shortest paths across each other as well as across metastasis and drug resistance markers, and the length were nearly uniform (Fig. 6C). SPHK1 had shortest paths of 2 across MMP-2, MMP-9, and ABCG2, whereas shortest path of 1 with ABCC1. Similarly, CERK also had shortest path length of 2 across MMP-2, MMP-9, ABCG2, and ABCC1. CERK was functionally close with other candidates, indicating it to be playing major role in maintaining the regulatory interactions during breast cancer. All these findings indicate the functional closeness of CERK and SPHK1 with metastasis and drug resistance markers and thus could regulate them through signaling pathways. Identification of smaller shortest paths between candidate genes also determined various transitive proteins (Supplementary Table S1) acting as important intermediates regulating the signaling during cancerous conditions.
Expression of MMP-2, MMP-9, ABCC1, and ABCG2 and their correlation with target genes
Analysis of gene and protein expression of metastasis markers
Tumor samples showed significant upregulation in the levels of MMP-9 (local cohort: (*p = 0.04); TCGA cohort: (***p < 0.0001) (Fig. 7B, D), while there was no difference in the levels of MMP-2 between tumor and adjacent normal tissue in local as well as TCGA cohort (Fig. 7A, C).
In the current studies, no significant difference in the protein expression of metastasis markers MMP-2 and MMP-9 was observed between tumor and adjacent normal tissue (Fig. 7E, F and G) (Supplementary Figs. 2 and 4).
Correlation of CERK and SPHK1 with metastasis markers
In order to establish the role of sphingolipids in metastasis, their correlation with metastasis markers was determined. It was observed that CERK had a positive association with both the metastasis genes MMP-2 (**p = 0.0011) (Fig. 8A) and MMP-9 (*p = 0.019) (Fig. 8B). Expression of SPHK1 was also found to be significantly correlated with MMP-2 (*p = 0.0179) (Fig. 8C) and MMP-9 (**p = 0.0071) (Fig. 8D). The correlation studies were further validated in the TCGA data cohort and similar results were found between the local and TCGA cohort. A significant positive association was observed between sphingolipid metabolism genes and metastasis markers (Fig. 8F, G and H) except for the CERK with MMP-2 (Fig. 8E). These observations suggest that these sphingolipid metabolites might have a potential role in breast cancer metastasis.
Analysis of gene and protein expression of drug transporters
Expression of two key drug transporters i.e., ABCC1 and ABCG2 were analysed in tumor and adjacent normal breast tissues in local as well as TCGA cohort. The level of ABCC1 was significantly upregulated in tumor tissue as compared to adjacent normal in local cohort (*p = 0.035) (Fig. 9A) and TCGA cohort (***p < 0.0001) (Fig. 9C). There was no statistically significant difference in the levels of ABCG2 between tumor and adjacent normal tissue in the local cohort (Fig. 9B) whereas in TCGA cohort, it was found to be significantly higher in normal tissue as compared to tumor tissue (***p < 0.0001) (Fig. 9D).
Protein expression analysis revealed that there was no significant difference in the levels of ABCG2 between tumor and adjacent normal tissues (Fig. 9E, F). The levels of ABCC1 were found to be significantly higher in tumor tissue as compared to adjacent normal tissue (**p = 0.0012) (Fig. 9G) (Supplementary Figs. 3 and 4).
Correlation of CERK and SPHK1 with drug resistance markers
The role of sphingolipids in drug resistance was examined by determining their correlation with drug resistance markers. It was revealed that CERK had a positive correlation with both the drug resistance genes ABCC1 (***p < 0.0001) (Fig. 10A) and ABCG2 (**p = 0.0033) (Fig. 10B). Similarly, SPHK1 was also found to be positively correlated to ABCC1 (**p = 0.0016) (Fig. 10C). No association was observed between the levels of SPHK1 and ABCG2 (Fig. 10D). The local cohort studies were further validated in the TCGA data set. It was found that both CERK and SPHK1 had a significant positive correlation with the drug transporter ABCC1 (*p = 0.0142) (Fig. 10E) and (***p < 0.0001) (Fig. 10G), respectively. However, for ABCG2, a negative association with CERK (***p < 0.0001) (Fig. 10F) and no association with SPHK1 was observed (Fig. 10H).
Expression analysis of SPNS2 and its correlation with SPHK1
The S1P transporter SPNS2 was also observed in the interaction network analysis (Supplementary Table S1). Since, SPNS2 was a transitive protein and not the primary interactor of target genes, its expression was analysed only in the TCGA cohort and was correlated with SPHK1. It was observed that the SPNS2 levels were downregulated in tumor tissue as compared to adjacent normal tissue (***p < 0.0001) (Fig. 11A). Correlation analysis revealed that the level of SPNS2 were positively associated with SPHK1 levels in breast tumor tissues (***p < 0.0001) (Fig. 11B).
Discussion
Sphingolipids represent the largest class of bioactive lipids associated with various aspects of tumorigenesis such as cell growth, proliferation, migration and drug resistance19,20. More recently, the clinical significance of sphingolipid-related biomarkers has acquired a forefront in cancer diagnosis and prognosis. In a previously published report, we found an elevation in the levels of sphingolipid metabolites CerP, S1P and SM in tumor tissues of breast cancer patients. There was also an upregulation in the expression of CERK and SPHK1 genes, involved in the synthesis of CerP and S1P metabolites. However, limited information is available on association of CERK and SPHK1 with clinical outcome in patient samples. In the present study, we aimed to determine association of these genes with clinicopathological characteristics as well as other cancer cellular processes in order to determine their clinical relevance in breast cancer.
CERK is a lipid kinase that regulates cellular processes such as proliferation, migration and inflammation. High expression of CERK has been reported to contribute to cell migration and invasion in metastatic breast cancer cells21. An earlier report has also highlighted the significance of CERK signaling in cancer migration and proliferation in human pancreatic cancer cells22. The inhibition of CERK by NVP-231 was found to decrease the rate of proliferation in breast and lung cancer cells23. CERK has also been reported earlier to promote tumor cell growth, survival and mammary tumor recurrence24,25. However, there is no study defining the significance of CERK among various clinical subgroups in breast cancer. The present study determined the association of CERK to various clinicopathological variables. We observed a significant upregulation in the expression of CERK in ER positive, nodal positive and late-stage breast cancer patients. A significant positive correlation was also observed between CERK levels and Ki67 index of breast cancer patients. High expression of CERK was also associated with poor OS in nodal positive and late-stage breast cancer patients. A previous study has also linked the increased expression of CERK with poor recurrence free survival in ER-negative breast cancer patients26. We further validated the gene expression results at post-transcriptional level. Consistent with the previous findings, increased protein expression of CERK was observed in tumor tissues as compared to adjacent normal tissues. Above studies therefore, reinforce the significance of CERK in discriminating the tumor tissue from adjacent normal tissues in breast cancer patients. High expression of CERK in lymph node positive and late-stage cancer patients and its association to poor OS further strengthen its potential as a prognostic biomarker in breast cancer patients.
The clinical correlations of SPHK1 were also assessed in the current study. Similar to the CERK, the levels of SPHK1 were found to be significantly high in patients with nodal metastasis and advanced stage of breast cancer. Our findings are in agreement with a previous study that reported an association between high SPHK1 expression and tumor size, lymph node metastasis and tumor-node-metastasis in papillary thyroid carcinoma27. The level of lymph node metastasis has been recognized as a major determinant of staging and prognosis in breast cancer28,29. Increased expression of SPHK1 was also found to corelate with clinical stage and distant metastasis of nasopharyngeal carcinoma30. In yet another finding, primary sites of colon cancers with metastases exhibited progressively high levels of SPHK1 than in those without metastases31. Inhibiting SPKH1 significantly decreased the serum S1P levels which in turn reduced tumor burden of the primary tumor, lymph node and lung metastases in murine model of metastatic breast cancer32.
Overexpression of SPHK1 has also been reported to significantly enhance the proliferation and invasion of papillary thyroid carcinoma cell lines27. An earlier study has also documented that increased SPHK1 expression is associated with increased Ki-67 in human nasopharyngeal carcinoma specimens33. In line with the previous studies, we also observed a positive significant association between SPHK1 expression and the proliferation marker Ki67. Similarly, a recent finding suggests that oral squamous cell carcinoma patients with high SPHK1 expression exhibit a higher Ki-67 labeling index than the patients with low SPHK1. However, the authors failed to establish the statistical association between SPHK1 and Ki-67 index34. In addition to their role in proliferation and invasion, increased expression of SPHK1 also contributes to poor OS of triple-negative breast cancer patients35. Other studies have also demonstrated an association between high SPHK1 levels and poor prognosis in patients with glioblastoma36 or breast cancer37. The elevated levels of SPHK1 were also found to correlate with shorter OS in hepatocellular carcinoma38. In the present study, the expression of SPHK1 did not show any impact on the overall prognosis of the disease. Consistent with the gene expression analysis, the present study also reports a significant elevation in the levels of SPHK1 proteins in tumor tissues as compared to adjacent normal. Similar to our findings, SPHK1 protein expression was found to be significantly upregulated in hepatocellular carcinoma cell lines38. The above observations clearly indicate that SPHK1 exhibit a good diagnostic ability in differentiating the lymph node metastasis and TNM stages and might serve as a potential biomarker to predict the clinical outcome in breast cancer patients.
The results of the current studies however, could not be validated in TCGA cohort wherein, no correlation was observed between the expression of CERK, SPHK1 and various clinical subgroups. The lack in significance could be attributed to racial/ethnical disparity of the TCGA patient cohort. A recent study also found that for the majority of cancers, there was a difference in the clinical characteristics between TCGA and general population cancer39.
To establish the significance of sphingolipids in drug resistance and metastasis of breast cancer patients, a regulatory network of SPHK1 and CERK with metastasis and drug resistance markers was inferred and analyzed. CERK and SPHK1 had smaller shortest paths across each other as well as across MMP-2, MMP-9 and ABCC1, ABCG2. Importantly, CERK was not highly connected nor was highly between in the network, but it was functionally close with other candidates, indicating it to be playing major role in maintaining the regulatory interactions during breast cancer. Overall, network analysis showed that all of the six candidate genes were closely correlated and might regulate each other during cancer processes. The results of the above observations were further validated in local as well as TCGA cohort.
Association of CERK and SPHK1 with metastasis markers
In the present study, we measured the expression of MMP-2 and MMP-9 in breast cancer patients and determined their correlations with SPHK1 and CERK in local as well as TCGA cohort. Expression of MMP-2 was not found to be differentially regulated between the tumor and adjacent normal tissue in both the cohort. These observations are in alliance with a recent finding that showed no significant difference in MMP-2 levels between breast cancer and normal tissues40. On the contrary, the levels of MMP-2 were significantly upregulated in colorectal cancer tissues than in normal tissues41.
The current study reports significantly high levels of MMP-9 in tumor tissues as compared to adjacent normal tissues in local as well as TCGA cohort. Similarly, a significant elevation in MMP-9 expression has also been reported in tumoral tissues than adjacent non-tumoral tissues in breast cancer40 and papillary thyroid cancer patients42. However, there was no difference in the protein expression of MMP-2 and MMP-9 between tumor and adjacent normal tissues. The lack in significance may be attributed to small sample size of the study.
To establish the role of sphingolipids in breast cancer metastasis, we analyzed the associations of MMP-2 and MMP-9 with SPHK1 and CERK expression. In local cohort, there was a significant positive correlation of SPHK1 and CERK levels with MMP-2 and MMP-9. Similar set of results were also observed in TCGA cohort except for the correlation of CERK with MMP-2. Evidence from past literature demonstrates that SPHK1 enhances the production of MMP-2 and MMP-9 and thereby promotes tumor proliferation and invasion of colon cancer9. High expression of SPHK1 was also shown to increase MMP-2/MMP-9 mRNA levels in head and neck squamous cell carcinoma43. However, to our knowledge, no previous report has determined the association of CERK with MMP-2 and MMP-9. These findings clearly suggest that CERK and SPHK1 might contribute to the breast cancer metastasis by modulating the expression of MMP-2 and MMP-9.
Association of CERK and SPHK1 with drug resistance markers
A plethora of mechanisms are reported to be involved in the process of MDR of which the best described is overexpression of members of the ABC transporter superfamily. Evidence from previous research stipulate ABCB1, ABCC1 and ABCG2 as the most likely targets in the MDR development5,44. To confirm the above hypothesis, we analyzed the expression of two drug transporters i.e., ABCC1 and ABCG2 in breast cancer patients and determined their association with SPHK1 and CERK in local as well as TCGA cohort. A significant upregulation of ABCC1 was observed in tumor tissues in both local as well as TCGA cohort. The protein levels of ABCC1 were also found to be high in tumor tissues as compared to adjacent normal tissues. Similar to our findings, high levels of ABCC1 in lymph node positive patients were suggestive of their potential role in breast cancer metastasis45. Another recent study in breast cancer found that expression level of ABCC1 in tumor tissue of patients responding to chemotherapy was significantly lower compared to non-responding patients46. Elevated levels of ABCC1 have been reported to be associated with clinical drug resistance in several malignancies such as lung, esophageal, leukemia and childhood neuroblastoma47,48,49,50. These observations suggest that ABCC1 can be envisioned as likely determinant of treatment response to various chemotherapeutic drugs in breast cancer patients.
In the current study, the levels of ABCG2 were downregulated in tumor tissues in local as well as TCGA cohort. However, the difference was not statistically significant in local cohort studies. Further, there was no difference in the protein expression between tumor and adjacent normal breast tissues. Albeit, small sample size of the local cohort is the main limitation associated with this study. Further investigations with large cohorts will be needed to illustrate the clinical utility of ABCG2 in breast cancer patient population.
Correlation studies revealed that ABCC1 levels were positively associated with CERK and SPHK1 expression in both the cohorts. A recent study has also shown that increased export of S1P via ABCC1 enhances the transcription of SPHK1 resulting in more S1P formation in human MCF-7 breast cancer cells51. For ABCG2, a positive correlation with CERK in local cohort and a negative correlation in TCGA cohort was observed. However, no significant correlation was observed between ABCG2 and SPHK1 in both the cohorts.
Interaction network analysis also led to the identification of S1P transporter SPNS2. The levels of SPNS2 were found to be downregulated in tumor tissue as compared to adjacent normal tissue in TCGA cohort. Similar to our findings, a recent study suggests that SPNS2 expression was markedly lower in ten kinds of tumor (including breast invasive carcinoma) as compared to adjacent non-cancerous samples. The authors reported that the levels of SPNS2 were downregulated in colorectal cancer and were associated with poor differentiation, advanced pTNM stage and poor prognosis of the disease52. Being a S1P transporter, the levels of SPNS2 were found to be positively associated with SPHK1 expression in tumor tissue in the current study. However, high expression of SPHK1 and low expression of SPNS2 in tumor tissue suggest that more S1P is being made but not transported from the cell via SPNS2. Since the current study reports the role of S1P in tumor proliferation, we hypothesize that S1P is being transported from the cell via other transporters. The possible mechanism for S1P export might be through ABC transporter ABCC1 as it is also upregulated in the current study. Similar observations were reported in an earlier study, where export of S1P via ABCC1 was found to amplify the S1P axis involved in breast cancer progression and metastasis51. However, future in vitro studies are required to explore the role of SPNS2 and its association to S1P axis in breast cancer patients.
Numerous studies have elucidated dysregulations in the levels of sphingolipid metabolites in various cancer types. However, the clinical relevance of sphingolipids as biomarkers in breast cancer patients remains elusive. The present study underscores the significance of sphingolipids in breast cancer patients in terms of diagnosis, prognosis and clinical correlations. High levels of SPHK1 and CERK in nodal positive and late-stage cancer patients as well as association of high CERK expression to poor patient survival are indicative of their potential as diagnostic and prognostic biomarkers. In addition, positive correlation of sphingolipid genes with Ki67 index, metastasis and drug resistance markers reinforces the assumption that these sphingolipids might have a predictive role in breast cancer proliferation, migration and drug resistance.
We acknowledge that small sample size of the local cohort is the main limitation of the present study. However, we have tried to validate gene expression analysis in large TCGA cohort. We were also unable to correlate the gene or protein expression with the patient survival in local cohort due to lack of follow up data. Further investigation in another independent large cohort is essential to confirm the present findings. Additionally, mechanistic evidences also need to be uncovered in order to exploit these findings for development of therapeutics.
Materials and methods
Chemicals and solutions
PureLink RNA isolation kit, RevertAid First Strand cDNA Synthesis Kit, DNase I enzyme, PowerUp SYBR Green Master Mix were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, United States). RNAlater, TRIzol Reagent and Bradford reagent were purchased from Sigma-Aldrich (St. Louis, Missouri, United States). Primers designed for the genes were purchased from Integrated DNA Technologies (Coralville, Iowa, United States). Primary antibodies against SPHK1 (NBP2-20472); CERK (NB100-2911); ABCC1 (NB400-156); ABCG2 (NBP2-22124); MMP-2 (NBP2-27208SS); MMP-9 (NBP2-13173SS) were purchased from Novus Biologicals (Centennial, Colorado, United States). Monoclonal primary antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (AM4300) was received from Thermo Fisher Scientific (Waltham, Massachusetts, United States). HRP-linked anti rabbit secondary antibody (7074) was purchased from Cell Signaling Technology (Danvers, Massachusetts, United States) and HRP- linked anti-mouse (SC-2005) was obtained from Santa Cruz Biotechnology (Dallas, Texas, United States). Clarity ECL Western Blotting Substrate was purchased from Bio-Rad (Hercules, CA, USA). All other chemicals used were of molecular biology grade.
Study design and ethics statement
The present study comprised of two different cohorts: local and TCGA. Both the cohorts involved the analysis of tumor and adjacent normal breast tissues. The study was performed in accordance with 1975 Declaration of Helsinki. Informed consent was obtained from the patients for the collection of specimens and clinical data. Ethical approval for the study was obtained from Institutional Ethics Committee, PGIMER (IEC-12/2017-787) and Panjab University Institute Ethics Committee (PUIEC/2019/154/A/01/03).
Sample collection
The tumor and adjacent normal tissues were obtained from the breast cancer patients who underwent surgical resection at Department of General Surgery, PGIMER, Chandigarh. The current study is an extension of our previous study wherein a total of 31 patients constituted the local cohort14. Clinical details were recorded and a pathological staging was done for all patients. Patient clinical characteristics included age, tumor type, pathological grade, pathologic T, N, M stage and Ki67 index. The TNM staging was done by the experienced pathologists according to the American Joint Committee on Cancer (AJCC) 8th edition. Breast tissues were also classified into estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (Her2) subtypes based on immunohistochemical (IHC) characteristics. The tissue samples were stored at − 20 °C in RNA later for RNA isolation and − 80 °C for protein isolation.
Histopathological analysis
The breast tissues were dissected, flushed with ice cold phosphate-buffered saline (PBS) and fixed in 10% neutral buffered formalin. Tissue was paraffin embedded and cut into sections of 5 µm thickness using a microtome. The sections were transferred to glass slides and stained with standard H&E - staining method. The slides were photomicrographed (3 random fields from each slide) using Nikon Eclipse 80i microscope (Nikon, Kawasaki, Japan).
RNA extraction and cDNA synthesis
Total RNA was extracted from the tissue samples using PureLink RNA Mini Kit (Thermo Fischer Scientific Waltham, Massachusetts, United States) according to the manufacturer’s instructions. The kit employs an organic extraction followed by immobilization of RNA on glass-fibre filters to purify total RNA. The purified RNA was stored at − 20 °C till further use. The integrity of RNA was checked on 2% agarose using gel electrophoresis. The concentration and purity of RNA was checked using NanoDrop 2000c Spectrophotometer. A total of 1 μg RNA was taken for cDNA synthesis. Prior to this, a treatment with DNase I enzyme was given to remove any possible genomic contamination. cDNA synthesis was carried out using Revert Aid First Strand cDNA synthesis kit purchased from Thermo Fischer Scientific (Waltham, Massachusetts, United States). The quality of the synthesized cDNA was checked by amplification of the housekeeping gene GAPDH.
Interaction network inference and analysis
A PPI network comprising CERK and SPHK1, metastasis markers and drug resistance markers was inferred by integrating physical PPIs and gene co-expression regulations among candidates as well as their corresponding interacting partners; physical interactions were obtained from the STRING database (string-db.org) while gene co-expression regulations were identified by processing gene expression data (BRCA-TCGA) obtained from the Broad Institute (gdac.broadinstitute.org). Topological analyses of the network were performed to assess the centrality of candidates based on their highest connectivity/degree (k), which represents network ‘hubs’, and BC which represents the network ‘bottlenecks’53. The biological relevance of centrality measures in prioritizing candidates was also evaluated by comparing the distribution of essential and non-essential genes in the interaction network. The shortest paths across CERK and SPHK1 and metastasis and drug resistance markers in the network were computed using the Dijkstra’s algorithm. All network analyses and visualizations were performed in R-igraph (v 1.2.6; igraph.org/r) and Cytoscape (v 3.8.2; cytoscape.org) software.
Primer designing for target genes
Primer designing for the genes of interest and the housekeeping gene was carried out using Primer-blast software. The primers were further assessed by Beacon Designer Free edition for the presence of secondary structures. The sequence of primers used is given in the Table 1.
Quantitative polymerase chain reaction (qPCR)
qPCR analysis was carried out to determine the mRNA expression of target genes in tumor and adjacent normal breast tissues. cDNA was diluted to a final concentration of 10 ng/μl. The annealing temperature for all the genes was standardized using gradient PCR. The reactions were performed in duplicates in Applied Biosystems Quant Studio 3 Real Time PCR System (Waltham, MA, USA) using Powerup SYBR green mastermix (Thermo Fisher Scientific, Waltham, Massachusetts, United States). The average Ct values for each sample were recorded and normalized with GAPDH as endogenous control. The relative gene expression was calculated using 2−ΔΔCt method.
Protein expression analysis
The expression of target proteins was studied using the standard western blotting method given by Towbin et al.54. The extraction of total proteins from the tumor and adjacent normal breast tissues was carried out in ice cold RIPA buffer containing 0.2% EZBlock Universal Protease and Phosphatase Inhibitor Cocktail (BioVision, Inc. Milpitas, CA, United States). The protein concentration of total cell lysates was determined by standard Bradford method55. A total of 50 μg of protein was separated on 10% SDS–polyacrylamide gel and electro-transferred to polyvinylidenedifluoride membranes (Bio-Rad, Hercules, United States). After blocking with 3% BSA, the membranes were incubated with primary antibodies for SPHK1 (1:1000), CERK (1:1000), MMP-2 (1:1000), MMP-9 (1:1000), ABCC1 (1:500), ABCG2 (1:1000) and GAPDH (1:2500) overnight with shaking at 4 °C. The membranes were then incubated with horseradish peroxidase (HRP) labeled goat anti-mouse (1:3000) and antirabbit (1:5000) secondary antibodies at room temperature for 1 h. The immunoblots were visualized on Image Quant LAS 500 (GE Healthcare, Chicago, Illinois, United States) using Enhanced Chemiluminescence Detection Kit. Densitometry analysis was performed using the AlphaView Software. Since the blots were cut prior to hybridization with antibodies, it was difficult to provide full length membrane images.
TCGA gene expression
Gene expression data pertaining to breast invasive carcinoma (TCGA abbreviation BRCA) was retrieved from the Firehose portal of Broad Institute (gdac.broadinstitute.org); this dataset comprises lowest-normalized level-3 microarray data (Agilent 244 K [G4502A]) from 529 tumor and 61 adjacent normal tissues. All statistical analysis and image rendering were performed in R 3.4.4 statistical environment (www.r-project.org).
Statistical analysis
Statistical analysis was performed using Graphpad Prism 5 software (La Jolla, CA, United States), SPSS statistics 21(IBM, Armonk, NY, United States) and MedCalc for Windows, version 15.0 (Ostend, Belgium). The continuous data were represented as Mean ± SEM. To detect and exclude any potential outliers, we considered the following points (1) either the expression values in tumor and normal are greater than value 75th + 1.5 × (value75th − value 25th) or (2) lesser than value 25th − 1.5 × (value 75th − value 25th).
For intergroup comparisons, Wilcoxon t-test (non-parametric) or paired t-test (parametric) was used to calculate the difference in expression between tumor and adjacent normal tissues. To compare sub-groups within same group and to calculate difference between the expression of tumor and normal samples in TCGA datasets Mann–Whitney U test (non-parametric) or unpaired t-test (parametric) was used. Correlations were carried out using Pearson's (parametric) or Spearman's (non-parametric) coefficient. Kaplan–Meier survival curves and log-rank test were used for survival analysis in TCGA data. All p values were two-sided and were considered significant when < 0.05.
Data availability
The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
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Acknowledgements
We thank Indian Council of Medical Research (ICMR) for providing fellowship to Ms. Priyanka Bhadwal.
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P.B. performed the research work, data analysis and wrote the manuscript. V.R. performed the computational network analysis. K.V. assisted in the histopathological analysis of the samples. D.D. provided the samples and clinical inputs for the study. N.A. conceived and supervised the project and refined the drafted manuscript.
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Bhadwal, P., Randhawa, V., Vaiphei, K. et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance. Sci Rep 12, 18239 (2022). https://doi.org/10.1038/s41598-022-20976-0
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DOI: https://doi.org/10.1038/s41598-022-20976-0
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