Abstract
The integrated stress response (ISR)-activated transcription factors ATF4 and CHOP/DDIT3 may regulate oligodendrocyte (OL) survival, tissue damage and functional impairment/recovery in white matter pathologies, including traumatic spinal cord injury (SCI). Accordingly, in OLs of OL-specific RiboTag mice, Atf4, Chop/Ddit3 and their downstream target gene transcripts were acutely upregulated at 2, but not 10, days post-contusive T9 SCI coinciding with maximal loss of spinal cord tissue. Unexpectedly, another, OL-specific upregulation of Atf4/Chop followed at 42 days post-injury. However, wild type versus OL-specific Atf4−/− or Chop−/− mice showed similar white matter sparing and OL loss at the injury epicenter, as well as unaffected hindlimb function recovery as determined by the Basso mouse scale. In contrast, the horizontal ladder test revealed persistent worsening or improvement of fine locomotor control in OL-Atf4−/− or OL-Chop−/− mice, respectively. Moreover, chronically, OL-Atf−/− mice showed decreased walking speed during plantar stepping despite greater compensatory forelimb usage. Therefore, ATF4 supports, while CHOP antagonizes, fine locomotor control during post-SCI recovery. No correlation between those effects and white matter sparing together with chronic activation of the OL ISR suggest that in OLs, ATF4 and CHOP regulate function of spinal cord circuitries that mediate fine locomotor control during post-SCI recovery.
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Introduction
The integrated stress response (ISR) is activated by a spectrum of cell damage-associated stimuli including protein misfolding in the endoplasmic reticulum (ER), protein aggregation in the cytosol, mitochondrial dysfunction, oxidative stress and amino acid deficiencies1. The two principal arms of the ISR include inhibition of general protein synthesis and induction of a transcriptional response that is mediated by several transcription factors including ATF4 and CHOP/DDIT31. Both arms are activated by phosphorylation of the translation factor eIF2α that reduces general protein synthesis while increasing translation of select mRNAs such as Atf4 or Chop1. ATF4 is the major mediator to the transcriptional arm of the ISR1,2,3,4. While ATF4 partners with CHOP to co-regulate many ISR-induced genes, it also directly stimulates Chop gene transcription1,2,3,4. The ATF4-driven gene expression program includes genes involved in restoration of cellular homeostasis such as components of the anti-oxidant defense systems, proteostasis, amino acid transport/synthesis and translation2,5. However, ATF4/CHOP-mediated upregulation of genes from the two latter categories may result in cytotoxicity5,6. Specifically, the resulting recovery from the ISR-mediated translational inhibition favors survival of cells with successful restoration of cellular homeostasis as increased translation under persistent stress becomes toxic5,6. In the case of persistent ER stress, nascent protein overload of the malfunctioning ER results in ER-based production of reactive oxygen species (ROS) and subsequent damage of the ER-associated mitochondria5,6. Such cytotoxicity may be further promoted by CHOP-regulated expression of pro-apoptotic genes3. Noteworthy, CHOP may also serve as a negative regulator of at least some ATF4-induced genes2. Therefore, CHOP plays a complex role in the transcriptional arm of the ISR by being an ATF4 partner in gene upregulation events, mediating a counterregulatory feedback to limit ATF4 activity, and also, reducing gene expression by inhibiting other transcription factors such as CEBPB2.
White matter damage after traumatic spinal cord injury (SCI) is associated with axonal degeneration, death of oligodendrocytes (OLs) and demyelination7,8. In contusive SCI at thoracic level, white matter damage is the main driver of SCI-associated loss of hindlimb function9. Thus, reducing OL loss is a major target for acute neuroprotection in these injuries. Various white matter pathologies such as SCI, experimental autoimmune encephalomyelitis (EAE) or some types of degenerative leukodystrophies are associated with activation of the transcriptional arm of the ISR in various neural cells including OLs, astrocytes, microglia, neurons, and microvasculature10,11,12,13,14,15,16. However, the outcome of such a response may vary dependent on the ISR mediator as well as the pathological and/or cellular context. In a mouse model of the degenerative leukodystrophy Pelizeus-Merzbacher disease (PMD), general Chop gene deletion (Chop−/−) exacerbated OL apoptosis and demyelination15. Moreover, while OL-specific overexpression of Chop did not cause any loss of those cells or abnormalities in myelin development, it had no modifying effects on white matter pathology in PMD mice17. In contrast, Chop−/− mice showed reduced white matter damage and improved locomotor recovery after moderate contusive T9 SCI11.
A major limitation of experiments using general, all-cell null mutants of Chop or Atf4 is the inability to directly identify the cell types in which those ISR mediators regulate white matter damage. After SCI, CHOP accumulates in OLs, but also in neurons, astrocytes and microvasculature10,11,14. Therefore, improved post-SCI survival of OLs in Chop−/− mice may result from dampened ISR-associated transcription in OLs and/or other neural cells. Indeed, in vanishing white matter disease, in which ATF4 is activated in astrocytes and microglia, astrocytes drive white matter pathology by reducing OL maturation16,18. The current study used OL-Atf4−/− and OL-Chop−/− mice to determine whether OL activity of those principal mediators of the transcriptional arm of the ISR modulates white matter damage and functional recovery after contusive SCI.
Results
Transient versus persistent activation of the ATF4/CHOP-driven transcription in OLs after moderate contusive SCI
Previous reports relied on CHOP immunostaining to show acute, SCI-associated activation of the transcriptional arm of the ISR in OLs10,11. However, neither duration nor transcriptomic scope of such a response was described. Therefore, we analyzed a SCI RNASeq dataset from OL-specific RiboTag mice to determine OL vs. total spinal cord tissue expression of Atf4, Chop as well as their downstream target gene transcripts. The dataset represented translatomes (i.e. polysome-associated mRNAs) from all cells (total RNA) or from mature OLs (OL RNA) that survived a moderate contusive T9 SCI (50 kdyn, IH). The tissue analyzed included a 5 mm spinal cord segment with the injury site in the center (see Methods for more details). Consistent with previous reports, total spinal cord expression of Atf4 or Chop increased acutely 1.5- or twofold of uninjured controls at 2 days post-injury (dpi), respectively (Fig. 1, q < 0.001). Conversely, subacute (dpi 10) or chronic (dpi 42) expression of those ISR mediators was similar to controls (Fig. 1, q > 0.05). In OLs, both Atf4 and Chop were upregulated on dpi 2 and dpi 42, but not dpi 10 (Fig. 1). Acutely, OL levels of Atf4 or Chop reached 3.25- or 2.4-fold of controls, respectively (Fig. 1, q < 0.001). Chronically, OL Chop was up 2.1-fold of control (Fig. 1b, q < 0.001). The OL Atf4 increase at that time was modest, but significant (1.3- fold of control, Fig. 1b, q < 0.001).
Next, the translatome datasets were analyzed to determine SCI effects on expression of genes directly regulated by ATF4 and/or CHOP in mouse fibroblasts with activated ISR5. Among OL upregulated genes (≥ 1.41-fold of control, q < 0.05), ATF4 targets were moderately overrepresented on dpi 2 but not dpi 10 or dpi 42 (Fig. 1c, 1.4-fold of the expected random overlap, p < 0.05). CHOP targets were overrepresented on dpi 2 and dpi 42, but not dpi 10 (Fig. dc, 1.7- or 1.8-fold overrepresentation on dpi 2 or 42, respectively, p < 0.01). Comparing the kinetics of SCI-associated expression changes, most of the OL-upregulated ATF4 targets (45 of 49 genes) clustered into one of 4 groups (Fig. 1d). While 15 genes were upregulated only on dpi 2 (cluster 1), 30 were also up on dpi 42 (clusters 2–4). Of them, 24 were upregulated at both timepoints, including 14 with a biphasic regulation (cluster 2, Fig. 1d) and 10 that were also up on dpi 10 (cluster 3, Fig. 1d). A similar clustering pattern was found for OL-upregulated CHOP targets (14/41 or 27/41 targets in cluster 1 or clusters 2–4, respectively, Fig. 1d). Co-immunostaining analysis using an antibody for the mature OL marker epitope CC1 confirmed OL expression of protein products of at least two ATF4/CHOP target genes that showed prolonged OL upregulation. Specifically, on dpi 42, signals for ATF5 or GPX4 were observed in CC1+ OLs from the spared white matter 1 mm rostral and caudal from the injury epicenter (Supplementary Figs. S1 and S2). While these data confirm acute upregulation of the transcriptional arm of the ISR in OLs, they also uncover unexpected persistence and/or re-activation of that pathway throughout the chronic phase of SCI recovery. As after contusive SCI in rodents, most OL loss occurs acutely with some OL apoptosis continuing subacutely7,8, chronic activation of OL ISR suggests its potential role in regulation of processes other than cell death. Those could include repair and/or re-organization of the white matter and/or maintenance of spared axon function.
Limited effects of OL-specific knockouts of Atf4 or Chop on expression of OL marker mRNAs acutely after SCI
To determine an OL cell autonomous role of ATF4 and CHOP in SCI pathogenesis, mice with OL-selective deletion of Atf4 (OL-Atf4−/−) or Chop (OL-Chop−/−) were used. Such mutations were generated by tamoxifen treatment (1 mg i.p. daily for 8 days) of double transgenic mice that combined the Plp1 promoter-driven CreERT2 recombinase with floxed alleles of Atf4 or Chop19,20,21. Littermates of OL-Atf4−/− or OL-Chop−/− mice that received vehicle instead of tamoxifen were used as wild type (wt) controls. Two weeks after the last tamoxifen injection, SCI was performed (IH 50 kdyn at T9) and mRNA expression of Atf4, Chop as well as their select target genes was evaluated at 6 or 72 h (dpi 3). This gene deletion verification study was limited to injured mice as in the intact CNS, basal expression of ISR genes including Atf4 or Chop is expected to be low. At 6 h after SCI, Atf4 or Chop mRNAs decreased by about 20% in OL-Atf4−/− or OL-Chop−/− mice, respectively (Fig. 2a, c, p < 0.05). That early reduction is consistent with the estimated 20–25% contribution of mature OLs to the total spinal cord cellularity22,23 and the SCI-associated ISR activation in all major types of neural cell10,11. In addition, efficient, OL-specific KO of Atf4 and Chop is supported by similar declines in total spinal cord expression of other broadly expressed genes deleted in OLs using the Plp1-CreERT2 line24. However, on dpi 3, total spinal cord expression of Atf4 or Chop was unaffected by their respective OL-KOs (Fig. 2a,c, p > 0.05). Such findings are likely due to SCI-induced changes of spinal cord cellular composition and/or high levels of ISR activation in non-OL cells at later times post-injury. Thus, on dpi 3, a reduced content of mature OLs and increases in oligodendrocyte precursor cells (OPCs), vascular cells and innate immune cells coincides with ISR activation in those expanding cell populations7,8,25,26,27. Therefore, on dpi 3, altered ISR activity in surviving OLs from OL-Atf4 or OL-Chop null mice may be hard to detect in total spinal cord RNA samples. Consistent with such interpretation, select ATF4 and/or CHOP target gene transcripts were also affected by OL-Atf4 or OL-Chop deletion at 6 h, but not 3 days post SCI (Fig. 2b,d and Supplementary Fig. S3). Thus, Chop and Me1 mRNAs were significantly decreased in OL-Atf4−/− mice (Fig. 2b). Conversely, Iars, Aldhl12 and Me1 were upregulated in OL-Chop−/− mice (Fig. 2d). Therefore, in OLs, CHOP may be a negative regulator of at least some ISR genes.
Importantly, at least for OL-Chop−/− mice, efficient and specific deletion of the floxed exon 3 of the Chop gene was also confirmed by qPCR analysis of genomic DNA (Fig. 2e,f, Supplementary Fig. S4). For those assays, spinal cord or liver DNA was isolated from naïve mice 2 weeks after completion of the tamoxifen induction protocol. As compared to vehicle-treated littermate controls (wt), OL-Chop−/− spinal cord DNA showed a 4.6-fold increase in Cre-dependent recombination inside the Chop gene that was accompanied by a 38% loss of the floxed exon 3 (Fig. 2f, p < 0.05). Conversely, no change in Chop gene recombination was observed in OL-Chop−/− versus wt liver where OLs are absent. Of note, in the spinal cord of wt mice, there was low, but noticeable, baseline recombination of the Chop gene that is likely related to tamoxifen-independent activity of Cre-ERT2 (Supplementary Fig. S4). Such activity has been reported before for various CreERT2 mouse lines28,29. Given similar effects of OL-Chop or OL-Atf4 knock out on mRNA expression of the respective floxed gene (i.e. Chop or Atf4, respectively, Fig. 2a,c), one can expect that Cre-mediated deletion of Atf4 is as efficient and specific as that observed for Chop.
On dpi 2–3, declining expression of OL marker transcripts in the contused spinal cord tissue is a correlate of acute OL loss and SCI-associated white matter damage30. However, on dpi 3, the OL mRNAs Aspa, Opalin, or Cnp showed similar expression in wt vs. OL-Atf4−/− or OL-Chop−/− mice (Fig. 2g,h). Therefore, SCI-induced acute loss of OLs seems to be unaffected by OL-selective deletion of Atf4 or Chop. Likewise, neither neuronal loss nor reactive astrogliosis appeared to be altered as expression of neuronal or reactive astrocyte marker transcripts was similar between OL-KOs and their respective wt controls at least on dpi 3 (Fig. 2g,h). Therefore, OL-selective loss of Atf4 or Chop does not appear to modulate acute loss of OLs or neurons.
Delayed modulation of functional recovery after SCI in OL-Atf4−/− and OL-Chop−/− mice
After SCI, locomotor recovery was evaluated over a 6-week period using the BMS on dpi 3, 7 and then weekly afterwards), horizontal ladder walking (dpi 14, 28, and, 42), and Treadscan® gait analysis (dpi 42). For BMS and ladder walking, baseline values were recorded before SCI but showed no significant differences between wt and OL-KOs. After SCI, BMS revealed strong improvement of hindlimb function over time, but no significant effects of either OL-Atf4−/− or OL-Chop−/− genotype (Fig. 3, see Supplementary Table S3 for detailed results of statistical analyses). Moreover, BMS subscores of these OL-ISR mutants did not differ substantially from wt controls except a significant decline in OL-Atf4−/− mice on dpi 14 (Fig. 3). Significant differences emerged in performance of the horizontal ladder test. On dpi 14, 28, or 42 OL-Atf4−/− mice did 57, 126, or 73% more ladder crossing errors than wt controls, respectively (Fig. 4a, p < 0.05, see Supplementary Table S3 for detailed results of statistical analyses). Conversely, 42 or 38% less errors were observed in OL-Chop−/− mice on dpi 28 and 42 but not on 14, respectively (Fig. 4b, p < 0.05). Finally, on dpi 42, gait analysis using the Treadscan® system uncovered significant changes in locomotor recovery of OL-Atf4−/−, but not OL-Chop−/− mice (Fig. 5, Supplementary Fig. S5). Those included lower average speed of walking with plantar stepping, reduced ratio of hindlimb plantar stepping cycles to forelimb plantar stepping cycles (plantar stepping index, PSI), shortening of the rear track width as well as shortening of forelimb stride length and swing time (Fig. 5, p < 0.05, see Supplementary Table S4 for detailed results of statistical analyses). Those latter effects suggest increased compensatory usage to cover for greater impairment of hindlimbs. Given the indirect evidence of greater hindlimb dysfunction as suggested by lower walking speed and increased use of forelimbs, significant narrowing of the rear track suggests reduced balance control in OL-Atf4−/− animals (Fig. 5c, p < 0.05). Such a deficit, together with decreased forelimb-hindlimb coordination (lower PSI), could have contributed to worsened ladder test performance of those mice after SCI. In addition, proficient ladder walking requires trunk stability, sensory feedback and control of the digits31. Hence, the opposing ladder walking phenotypes of OL-Atf4−/− or OL-Chop−/− mice may reflect distinct modulatory effects on such modalities of fine locomotor control.
As all those functional analyses included mice of both genders, additional statistical analyses were performed that included gender as a factor in ANOVA design. However, none of the recovery parameters analyzed showed a significant effect of gender (e.g. for OL-Atf4−/− study, ladder test errors: F = 0.10, df = 1,25, p = 0.749, and BMS: F = 0.01, df = 1,37, p = 0.932; for OL-Chop−/− study, ladder test errors: F = 0.02, df = 1,37, p = 0.898 and BMS: F = 2.9, df = 1,30, p = 0.10). However, as low numbers of animals were used, the gender effect analysis is underpowered. Therefore, potential gender-specific modulation of post-SCI recovery by the transcriptional arm of the OL-ISR should be addressed by future studies.
SCI-induced white matter injury is unaffected in OL-Atf4−/− and OL-Chop−/− mice
On dpi 42, white matter sparring was analyzed using EC staining of myelin. At the injury epicenter, 70–75% of the myelinated white matter was lost in wt mice (Fig. 6, p < 0.05, see Supplementary Table S5 for detailed results of statistical analyses). Similar damage was observed in OL-KOs (Fig. 6, p > 0.05). In addition, OL-KOs showed unaffected white matter content 1 mm rostrally or 1 mm caudally from the epicenter (Fig. 6, p > 0.05). As the epicenter loss of myelinated white matter is a key correlate of hindlimb function that is determined by BMS9, these histological results are consistent with unaffected BMS scores in OL-Atf4−/− or OL-Chop−/− mice.
Finally, the relative content of all OL-lineage cells (OPCs and OLs) or mature OLs was analyzed in the spared, ventral white matter using OLIG2 or CC1 immunostaining, respectively (Fig. 7a,b). In all genotypes, OL/OPC content was lower at the injury epicenter than 1 mm rostrally or caudally from that region (Fig. 7c–f, p < 0.05; see Supplementary Table S5 for detailed results of statistical analyses). These findings are consistent with a lasting OL loss in the spared white matter at the epicenter level following contusive SCI7,8. However, the epicenter OL/OPC content was unaffected by the OL-Atf4−/− or OL-Chop−/− genotype (Fig. 7c–f, p > 0.05). Interestingly, as compared to wt, OL-Atf4−/− mice showed reduced OL/OPC content in the ventral white matter 1 mm caudally, but not rostrally, to the epicenter (Fig. 7c,d, p < 0.05). No such effects were present in OL-Chop−/− mice (Fig. 7e,f). Overall, these histological analyses support a notion that at the injury epicenter, the lasting loss of spared white matter OLs is not affected by OL-selective deletion of Atf4 or Chop. Outside of that region, lost OLs are effectively replaced by new OLs that are produced by OPCs with OL content recovering to or above pre-injury levels by 4–5 weeks after contusive SCI7,8. Therefore, the lower caudal OL content in OL-Atf4−/− mice may reflect reduced generation and/or survival of new OLs. Alternatively, pre-existing OLs in the caudal white matter may be selectively dependent on ATF4 for survival in the injured spinal cord environment. Regardless of a mechanism, the functional significance of altered OL content in the caudal region of the spared ventral white matter is unclear.
Discussion
Current results reveal OL-cell autonomous roles of ATF4 and CHOP in functional recovery post-SCI. Unexpectedly, those roles are opposite and likely unrelated to a lasting loss of OLs or white matter sparing at the injury epicenter, as there were no apparent differences in latter processes between the two strains. Interestingly, the worsening or improvement of fine locomotor control in OL-Chop−/− or OL-Atf4−/− mice, respectively, persists through the chronic phase of recovery correlating with a delayed OL upregulation of ATF4/CHOP target genes. Therefore, chronic activation of the OL-ISR may modulate function of neuronal circuitries that mediate post-SCI recovery.
ATF4, and particularly CHOP, are often implicated as cell autonomous-mediators of stress-induced death and/or survival of neurons and OLs3,11,15,32,33,34,35,36. Therefore, we anticipated that OL-selective deletions of Atf4 and/or Chop will modulate SCI-associated acute death of OLs as well as their lasting loss and white matter damage at the injury epicenter. Instead, our data suggest that those pathological processes do not require OL-cell autonomous activities of ATF4 or CHOP.
While general Chop deletion has strong effects on OL loss, white matter sparing and functional recovery post-SCI11, OL-Chop−/− mice show functional recovery improvements that could be detected only on more complex sensorimotor tasks. Moreover, no significant effects on acute OL loss or chronic white matter sparring were found in OL- Chop−/− mice. Such differences suggest that CHOP activity in cells other than OLs is a positive regulator of acute white matter damage that, in turn, determines loss and recovery of hindlimb function. It is possible that those cells include microglia and/or monocyte-derived macrophages, which are key drivers of the cytotoxic neuroinflammation post-SCI26,37. Reduced macrophage-mediated inflammation has been reported in non-CNS injury models in Chop−/− mice or in Chop−/− macrophages in culture38,39,40,41. Therefore, targeting CHOP after SCI may exert pleiotropic benefits across several cell types including attenuation of microglia/macrophage-mediated white matter damage as well as enhancement of OL support for neuronal circuitries controlling fine locomotor control.
Although no contusive SCI data from Atf4−/− mice have been reported, such animals showed worsened locomotor BMS recovery following T12 hemisection SCI42. Attenuated increases in OL lineage cell numbers were also found42. Therefore, as compared to the current findings from OL- Atf4−/− mice, the SCI phenotype of the general Atf4 KO shows at least similar direction with respect to functional recovery and OL linage cell content.
Besides cell death and survival, the ISR regulates other aspects of cellular physiology including metabolism, stress adaptation, and cell–cell communication1,2,3,4. In neurons, the ISR may modulate the function of neuronal circuitries, including neuroplasticity and cognition43,44. Thus, persistent neuronal activation of the ISR kinase PERK/EIF2AK3 has been implicated as a driver of impaired cognition in a mouse model of Alzheimer’s disease (AD)45. Moreover, chronic activation of the ISR was found in mouse traumatic brain injury (TBI) and its pharmacological attenuation improved cognitive recovery46. Intriguingly, the current data suggest that in surviving OLs, the transcriptional arm of the ISR is activated chronically after SCI. In addition, SCI phenotypes of OL-Atf4−/− and OL-Chop−/− mice support a possibility that such a response regulates fine motor control during locomotor recovery in an ISR mediator-specific manner. Those findings raise an interesting possibility that the functional outcomes of prolonged ISR activation following CNS injury are determined by a stressed cell type and/or an ISR mediator.
What could be the potential mechanisms behind functional modulation of the fine locomotor control downstream of OL-ISR? One possibility is that the ISR in surviving OLs modulates remyelination. Post-SCI remyelination is mediated by new OLs that are generated from OPCs in response to injury7,8. Post-SCI oligodendrogenesis may lead to higher OL number especially in areas distal to the lesion site47. Hence, lower content of OLs/OL lineage cells in OL-Atf4−/− mice selectively at + 1 mm from the epicenter could suggest a location-specific and potentially non-cell autonomous requirement of OL ATF4 in OPC-mediated oligodendrogenesis including proliferation, differentiation and/or survival of newly generated OLs. In addition, pre-existing OLs may be capable of remyelination48,49. Hence, the transcriptional arm of the OL-ISR could also modulate this reparative process in a cell-autonomous manner. However, significance of post-SCI remyelination is unclear and studies using a mouse mutant that was not capable of remyelination suggests no major role of that process in functional recovery50. Current data are consistent with a limited role of OL-mediated remyelination in post-SCI recovery of hindlimb function, at least in a mouse model of contusive SCI with BMS as major functional readout.
In addition, OL ISR may modulate recovery of fine motor control by altering functional properties of spared myelin sheaths that wrap intact axons51,52. Both ATF4 and CHOP are well positioned to exert a broad control over cellular metabolism and potentially regulate OL function and/or OL-mediated support of axons. ATF4 is a positive regulator of a large group of genes involved in protein- and amino-acid synthesis2,5,6. Lipid synthesis is another critical component of myelin biogenesis and maintenance that may be positively regulated by ATF453,54,55. Moreover, ATF4 is a positive regulator of the lactate generating anerobic glycolysis, which is highly active in OLs to support axonal oxidative metabolism56,57. Therefore, ATF4 may not only support macromolecule synthesis that is required for repair and/or maintenance of myelin but may also drive OL production of axon supporting metabolites such as lactate.
As many target genes of ATF4 are co-regulated by CHOP, it may seem surprising that the functional recovery phenotypes of OL-Atf4−/− and OL-Chop−/− mice are mostly opposite. However, the ATF4/CHOP co-regulation data come from cultured cells whose ISR was activated by ER stress-inducing agents such as tunicamycin or thapsigargin5. In other contexts, including whole animal tissues and/or other ISR-inducing stimuli, CHOP may oppose transcription that is mediated by ATF4, including upregulation of pro-anabolic genes that support amino acid supply and protein synthesis58,59. Importantly, increased expression of select ISR-associated transcripts in OL-Chop−/− mice supports a CHOP-mediated feedback that restricts ATF4 activation in SCI-challenged OLs. Such feedback could explain opposite SCI recovery phenotypes in OL-Chop−/− and OL-Atf4−/− mice.
Together, current data suggest that in OLs, the transcriptional arm of the ISR that is mediated by ATF4/CHOP is not a major regulator of acute OL loss after SCI. Instead, it regulates fine motor control during later stages of the recovery correlating with its delayed activation in OLs. While OL-ATF4 promotes, OL-CHOP reduces that recovery. Therefore, enhancing activity of ATF4 and/or inhibiting CHOP offers potential strategies to improve functional outcome after SCI. The therapeutic window for such strategies may extend into the subacute and/or chronic phases of the recovery increasing their translational potential.
Materials and methods
Animals
The following mouse strains were obtained from the Jackson laboratories: C57Bl6, B6J.129(Cg)-Rpl22tm1.1Psam/SjJ (RiboTag+/wt, Strain # 029,977) B6.Cg-Ddit3tm1.1Irt/J (Chop fl/fl, Strain #030,816), B6.Cg-Tg(Plp1-cre/ERT)3Pop/J (Plp-creERT2, Strain # 005975). The Atf4 fl/fl mice with loxP sites flanking Atf4 exons 2–3 were generously provided by Dr. Christopher Adams (University of Iowa)20. The following mice were generated by crossing those parental strains: RiboTag:Plp-creERT2 (both transgenes at heterozygosity), Atf4fl/fl:Plp-creERT2 and Chop fl/fl:Plp-creERT2 mice (the Plp-creERT2 transgene at homozygosity). To induce OL-selective ribosome labeling (removal of a stop codon to produce HA-tagged Rpl22 in RiboTag mice) or deletions of Atf4fl/fl or Chopfl/fl loci, 6–8 week old mice of the respective genotypes received 8 daily i.p. injections of tamoxifen (1 mg/day)24,60. Littermates that received vehicle injection (sunflower oil) were used as wild type (wt) controls. Experiments using OL-RiboTag, OL-Atf4−/−, or OL-Chop−/− mice started 2 weeks (OL-ISR KOs) or 30 days (OL-RiboTag) after the last tamoxifen injection. Non-transgenic, female C57Bl6 mice were used for immunostaining analyses of ATF5 or GPX4 and as additional controls for validation of the Chop gene recombination assays. All animal experiments were approved by the University of Louisville Institutional Animal Care and Use Committee and Institutional Biosafety Committee and strictly adhered to NIH guidelines on use of experimental animals. In addition, ARRIVE guidelines were followed including random order of SCI or sham surgeries in animals from different experimental groups. However, fully randomized design of experimental groups was not possible due to limited availability of animals with required genotypes. Therefore, non-random group assignments were needed to obtain sufficient animal number for adequate statistical power and ensure balanced inclusion of both sexes.
Materials
Antibodies were obtained from commercial suppliers as indicated. All other reagents and materials were purchased from Sigma Aldrich (St. Louis, MO), VWR International (Radnor, PA), Thermo Fisher Scientific (Waltham, MA), or Integrated DNA Technologies (IDT, Coralville, IA).
Spinal cord injury
SCI was performed as described previously11. Briefly, 8–10 week old mice were anaesthetized by an i.p. injection of 400 mg/kg 2,2,2-tribromoethanol. Lacri-Lube ophthalmic ointment (Allergen, Irvine, CA) was applied to prevent drying of eyes. After a laminectomy at the T9 vertebral level, moderate contusion injuries (50 kdyn force/400–800 μm displacement) were performed at using the IH impactor (Infinite Horizons, Lexington, KY). Postoperative care included s.c. 0.1 ml saline (immediately after surgery), 0.1 mg/kg s.c. buprenorphine every 12 h for 2 days, and manual expression of bladders twice a day for seven to ten days or until spontaneous voiding returned. All surgical and post-operation procedures were completed according to NIH and IACUC guidelines.
Hindlimb locomotor function
Hindlimb function was evaluated in an open field using the Basso Mouse Scale (BMS)61. Evaluations were performed at baseline before the injury and weekly thereafter. All raters were trained by Dr. Basso and colleagues at the Ohio State University and were blinded to genotype or surgical identity. The horizontal ladder test was performed as described previously using Columbus Instruments Sensor and RS-232 Mini Counter (Columbus Instruments; Columbus, OH, USA, 2.5 mm rungs spaced 3.5 cm apart)62. Briefly, each animal underwent five stepping trials per session and the total number of footfalls was quantified for the left and right limbs, respectively. A baseline session before SCI was followed by bi-weekly assessments starting at 2 weeks post-injury. Gait was analyzed using the Treadscan® gait analysis system (Cleversys, Reston, VA) after completion of all locomotor assessments at week 6 after SCI as described previously63. Briefly, mice were placed on a variable speed treadmill with a clear belt, and a high-speed digital video (ventral view) of the stepping animals was recorded. Each animal was placed on the treadmill, the speed of the belt increased until the animal could no longer maintain position, and then decreased slowly until stable walking was achieved. Ten + consecutive step cycles were recorded at such individually optimized walking speed (if an animal was unable to execute 10 cycles, at least two 5 + step cycles were recorded). A minimum of 8 step cycles/animal were then analyzed using the Treadscan software.
Tissue collection and processing
Mice were deeply anaesthetized using 2,2,2-tribromoethanol and perfused transcardially with ice cold phosphate buffered saline (PBS, 30 ml/mouse). For RNA analysis, spinal cord samples (5 mm centered on the injury epicenter) were collected and frozen in liquid nitrogen. Samples for genomic DNA isolation were collected and stored in a similar manner. For histology, mice were perfused with 4% paraformaldehyde (PFA, pH 7.4 in PBS). Dissected spinal cords were post-fixed (4% PFA for 1 h at 4 °C), cryoprotected overnight in 30% sucrose (pH 7.4, 72 h at 4 °C) and mounted in TFM tissue freezing medium (GeneralData, Cincinnati, OH). Cryostat Sections (20 μm) were cut, thaw mounted on slides and stored at − 20 °C until further use.
Translatome analysis using RiboTag mice
After SCI, female OL-RiboTag mice were anesthetized and transcardially perfused with ice cold PBS. Dissected spinal cords (5 mm spanning the injury epicenter) were flash frozen in liquid nitrogen and stored at − 80 °C until further use. Two frozen SCI epicenter samples from SCI or naïve animals were pooled to produce one biological replicate (n = 3/group, 6 animals/group). SCI and naïve samples were processed for input polysome-associated mRNAs (IN) or immune-precipitated (IP) OL polysome mRNAs as previously described64. RNA isolation, library preparation and RNA sequencing on the Illumina NextSeq 500 platform were performed following standard procedures. Following Fast QC quality control, the reads were directly aligned to the Mus musculus reference genome assembly (mm10.fa) using STAR (version 2.6) followed by raw read count (HTSeq version 0.10.0). The number of raw reads/sample was 36,812,296 to 39,546,186 (average 37,495,614). At least 96.56% reads were aligned to the mouse reference genome (average read alignment 98.06%). The raw counts were normalized using the Relative Log Expression (RLE) method and then filtered to exclude genes with fewer than 10 counts across samples. Differential expression was analyzed using DESeq2. Pairwise differential expression for IN or IP comparing SCI samples to naïve samples was performed to identify changes in gene expression after SCI. Differential expression between IP and IN was also analyzed for each set of samples to determine OL-enriched expression in naïve or SCI mice. False discovery rate-corrected p-value (q) was calculated for each transcript with q < 0.05 to identify significant gene expression changes. In both naïve or dpi 2 groups, established marker transcripts for OLs (Mbp, Plp, Cldn11, Cnp) or neurons (NeuN/Rbfox3, Nefh, Nefl and Nefm) showed at least fourfold enrichment or depletion in IP vs. IN samples, respectively (q < 0.05), confirming successful enrichment of OL transcriptome in IP samples.
qPCR analysis of mRNA expression
RNA was extracted using Trizol or RNeasy Lipid Tissue Mini Kit (Qiagen, Germantown, MD) and cDNA was synthesized following standard methodology. SYBR Green master mix- or Taqman DNA polymerase and ViiA 7 Real-Time PCR System (Applied Biosystems, Grand Island, NY) were used for non-labeled or Taqman primers, respectively (see supplementary Table S2 for primer information). The ΔΔCT method was used for quantification; the normalizing transcript was Gapdh. Validation of gene knockouts was performed using custom designed primers to amplify the mRNA regions corresponding to the deleted exon/exons (Atf4 exons 2–3 or Chop exon 3, supplementary Table S2).
qPCR analysis of genomic DNA
DNA was isolated from frozen spinal cord or liver samples using AllPrep DNA/RNA Mini kit (Qiagen, Germantown, MD. Cat# 80204) according to manufacturer’s recommendations. All primmer sequences are listed in the Supplementary Table S2. To ensure specific amplification, DNA products of a regular PCR were separated using agarose gel electrophoresis and their number and size was determined. PCR (25 cycles) was performed using Promega 5XGreen GoTaq Flexi Buffer (cat# M891A), GoTaq Flexi DNA Polymerase (cat# M829B) and a minicycler PCR instrument (MJ Research INC, Watertown, MA). Then qPCR analyses were conducted for all amplicons using SYBR Green master mix and ViiA 7 Real-Time PCR System (Applied Biosystems, Grand Island, NY). Quality control for those assays included standard curves and melting curves of qPCR products. The ΔΔCT method was used for quantification with a normalizing amplicon from a non-recombining region of the Chop gene (amplicon 3).
WMS analysis
WMS was evaluated using iron eriochrome cyanine (EC) staining for myelin as described previously11. A detailed description is provided in Supplementary Methods.
Immunostaining
Slides with mounted spinal cord sections were warmed at 37 °C for 10 min, washed 3× with PBS, and blocked in TBS + 0.1% Triton X-100, 0.5% BSA, and 10% normal donkey or goat serum for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies in blocking buffer (mouse anti-APC, clone CC1 (1:200, Calbiochem, OP80), rabbit anti-OLIG2 (1:200, Millipore, Ab9610), followed by 3× washes and incubation in secondary antibodies at 25 °C for 1 h. Negative controls included appropriate species-specific non-immune Ig subtypes instead of primary antibodies. Cell nuclei were counter-stained with Hoechst-33258. Co-immunostanings for APC/CC1 (1:100, Calbiochem, OP80) and ATF5 (1:100, Novusbio, NBP2-92397) or GPX4 (1:100, Abcam, ab125066) were performed in a similar manner except use of 10% or 5% normal goat serum (in 0.1% Triton X-100, PBS) for blocking or primary antibody incubation, respectively.
Imaging
The white matter sparing images were captured with Nikon Eclipse Ti inverted microscope equipped using a 4× objective. The co-immunostaining images were captured with Nikon C2 confocal microscope using a 10× objective for cell counts. Identical acquision parameter were used for corresponding injured and control spinal cords. Digitized images were captured using NIS-Elements software Ar 5.3 followed by conversion to JPEG files.
OL content
Quantification of fluorescent immunostained images followed previously described methodology using transverse 20 μm-thick sections that were stained with OLIG2 (OL/OPC marker) or CC1 (OL marker) and counterstained with Hoechst-3325865.
Statistical analysis
Repeated measures ANOVA (RM-ANOVA) followed by Bonferroni post hoc tests was used for analyzing locomotor recovery (BMS, ladder test) and histological data (WMS, cell counts). Gait parameters were analyzed using RM-ANOVA/Bonferroni or t-test (two-tailed) as indicated. Transcript expression/genomic amplicon data (qPCR) were analyzed using the non-parametric Mann–Whitney test (u-test, two-tailed).
Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
This work was supported by NS103433 (MH, SRW), NS108529 (MH, SRW), Jewish Heritage Fund for Excellence Research Enhancement Grant (MH, SRW), Norton Healthcare (SRW) and the Commonwealth of Kentucky Challenge for Excellence (MH, SRW). Sequencing and bioinformatics support for this work was provided by P20 GM103436 and P20 GM106396 (ECR).
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Y.G., G.W., and M.D.F. designed and executed experiments, analyzed their results, and wrote the manuscript; B.R., E.R.H., D.B., K.A., J.M., C.A., C.G., L.P.S. and J.D. executed experiments and analyzed their results, J.H.C. and E.C.R. performed RNASeq data analysis, S.S.O. designed experiments and edited the manuscript, S.R.W. designed experiments, edited the manuscript and provided financial support, M.H. designed experiments, analyzed data, wrote the manuscript and provided financial support.
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Gao, Y., Wei, G.Z., Forston, M.D. et al. Opposite modulation of functional recovery following contusive spinal cord injury in mice with oligodendrocyte-selective deletions of Atf4 and Chop/Ddit3. Sci Rep 13, 9193 (2023). https://doi.org/10.1038/s41598-023-36258-2
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DOI: https://doi.org/10.1038/s41598-023-36258-2
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