Abstract
Nanophthalmos is characterised by shorter posterior and anterior segments of the eye, with a predisposition towards high hyperopia and primary angle-closure glaucoma. Variants in TMEM98 have been associated with autosomal dominant nanophthalmos in multiple kindreds, but definitive evidence for causation has been limited. Here we used CRISPR/Cas9 mutagenesis to recreate the human nanophthalmos-associated TMEM98 p.(Ala193Pro) variant in mice. The p.(Ala193Pro) variant was associated with ocular phenotypes in both mice and humans, with dominant inheritance in humans and recessive inheritance in mice. Unlike their human counterparts, p.(Ala193Pro) homozygous mutant mice had normal axial length, normal intraocular pressure, and structurally normal scleral collagen. However, in both homozygous mice and heterozygous humans, the p.(Ala193Pro) variant was associated with discrete white spots throughout the retinal fundus, with corresponding retinal folds on histology. This direct comparison of a TMEM98 variant in mouse and human suggests that certain nanophthalmos-associated phenotypes are not only a consequence of a smaller eye, but that TMEM98 may itself play a primary role in retinal and scleral structure and integrity.
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Introduction
Nanophthalmos is an inherited condition characterised by bilaterally small but structurally normal eyes, and associated with high hyperopia and an increased risk of angle-closure glaucoma. At least four genes have been associated with nanophthalmos, including recessive (PRSS56, MFRP) and dominant (TMEM98, MYRF) forms, with recessive forms phenotypically more severe than their dominant counterparts1.
Mouse models of human nanophthalmos are phenotypically diverse, and do not always mirror their human counterparts. A close murine counterpart to human nanophthalmos is the Prss56grm4/grm4 mutant, which exhibits reduced axial length, narrow drainage angles, ocular hypertension, and glaucomatous neurodegeneration2,3. Mouse Mfrp mutants, on the other hand, have a normal axial length, but are associated with white fundus flecks, progressive retinal degeneration, and retinal pigment epithelium atrophy4,5. Similarly, homozygous conditional deletion of Myrf in the developing retina was associated with patchy retinal degeneration, but no difference in ocular axial length6.
TMEM98 was first associated with nanophthalmos in a large European kindred segregating the p.(Ala193Pro) missense variant7. Although the significance of this association had been questioned8, reports of TMEM98 variants in multiple additional nanophthalmos kindreds have followed, including the p.(His196Pro) variant in a European family, and a 34 bp deletion spanning the 3’ end of exon 4 in a Micronesian family9. Subsequently, the same p.(Arg201Pro) variant has been reported in two apparently unrelated families in Austria10, and the US11. More common TMEM98 variants have also been associated with myopia in genome-wide association studies12,13.
To further investigate the role of TMEM98 in ocular development we used CRISPR/Cas9 editing to create a mouse model of human TMEM98-associated nanophthalmos. We report here the phenotypes observed in these mice, as compared to the effects of the corresponding variant in a human nanophthalmos case with the corresponding variant.
Results
To validate the functional impact of the human p.(Ala193Pro) variant of TMEM98, we used germline CRISPR/Cas9 editing to recreate the equivalent variant in C57BL/6J mice (Fig. 1). Genotyping of F1 progeny (n = 66) weaned from two Tmem98A193P/wt x Tmem98A193P/wt breeding pairs demonstrated a wild-type:heterozygote:homozygote ratio of 13:46:7, consistent with no intrauterine lethality (p = 0.06; chi-square test).
We next compared the phenotype of wild-type (Tmem98wt/wt), heterozygous mutant (Tmem98A193P/wt), and homozygous mutant (Tmem98A193P/A193P) mice against the clinical phenotype of a previously reported nanophthalmos case who was heterozygous for the equivalent variant (TMEM98A193P/+)7.
Consistent with other reports, our human TMEM98A193P/+ nanophthalmos case had very short axial lengths in both eyes (RE: 17.1 mm, LE: 17.14 mm), with corresponding high hyperopia (spherical equivalent RE: + 15.5 D, LE: + 15.0 D). However, globe size did not appear to be different in mutant mice, and there were no discernible differences between genotypes in the anterior segment appearance or globe contours, as assessed by two blinded examiners (Fig. 2a). Globe diameters in 7-month-old Tmem98wt/wt mice (3.4 mm ± 0.08; n = 4 eyes) did not differ from Tmem98A193P/A193P homozygotes (3.4 mm ± 0.04, p = 0.48; n = 8 eyes) nor Tmem98A193P/wt heterozygotes (3.5 mm ± 0.05, p = 0.77; n = 6 eyes) (Fig. 2b). Furthermore, corneal diameters of Tmem98wt/wt controls (3.36 mm ± 0.03; n = 4 eyes) did not differ from Tmem98A193P/A193P homozygotes (3.3 mm ± 0.04, p = 0.97; n = 8 eyes) nor Tmem98A193P/wt heterozygotes (3.34 mm ± 0.06, p = 0.26, n = 6 eyes) (Fig. 2c). No eyes were excluded from analysis.
In our human TMEM98A193P/+ nanophthalmos case, the maximum recorded intraocular pressure (IOP) (RE: 33 mmHg, LE: 24 mmHg) was elevated, with gonioscopic examination revealing angle closure, consistent with other reported in patients with TMEM98 variants7,9. The IOP in Tmem98A193P/A193P homozygous mice (10.5 mmHg ± 0.5) was not significantly different to Tmem98wt/wt controls (11.3 mmHg ± 0.2) or Tmem98A193P/wt heterozygotes (10.3 mmHg ± 0.4) (Fig. 2d). Subjective grading of anterior chamber depth using slit lamp examination also did not show a difference between genotypes.
Electron micrographic imaging of sclera (obtained post-mortem) in the human TMEM98A193P/+ nanophthalmos case (Fig. 3a) revealed disorganised collagen bands. Individual collagen fibers displayed wide variation in thickness when viewed in cross-section, and longitudinal sections occasionally displaying thickened bulbous diffuse formations with loss of banding where fibre integrity was disrupted. However, in the Tmem98 mouse line, all genotypes showed well-organised collagen fibrils with retained fibre integrity and banding (Fig. 3b). In Tmem98A193P/A193P mice the mean collagen fibril diameter (97.9 nm ± 2.5; n = 3 eyes) was not significantly different to Tmem98A193P/wt heterozygotes (98.5 nm ± 4.0, P = 0.73; n = 3 eyes) nor Tmem98wt/wt controls (105 nm ± 2.7, P = 0.73; n = 3 eyes) (Fig. 3c). The corneal collagen fibres in mice also showed no disorganisation or difference between genotypes (Fig. 3d). No eyes were excluded from analysis. These findings suggest that disorganised collagen in the human nanophthalmos case may arise due to abnormally shortened axial length, rather than a primary phenotype due to TMEM98-mediated interactions with collagen.
Finally, we compared the appearance of the retina in both human and mouse, using a combination of colour photography, histology, and/or ocular coherence tomography (OCT).
Colour photography of the retinal fundus in 7 month-old mice revealed prominent white dots in Tmem98A193P/A193P homozyotes, which were not apparent in Tmem98A193P/wt heterozygotes nor Tmem98wt/wt controls (Fig. 4A). These were also associated with outer retinal folds on histology, apparently at the level of the outer nuclear layer (Fig. 4A). We also observed retinal white dots in two human TMEM98A193P/+ cases: and in neither case were these white dots correlated with specific features on OCT (Fig. 4B). In at least one TMEM98A193P/+ case, we also observed bilateral superficial optic disc drusen, with a characteristic hyporeflective core on OCT (Fig. 4C)14.
Discussion
Here we perform a cross-species phenotypic comparison in human and mice harboring the same nanophthalmos-associated variant in TMEM98 (p.(Ala193Pro)). Despite many phenotypic differences, it is clear that TMEM98 is critical for ocular development in both species. In humans, the p.(Ala193Pro) variant can clearly act in a dominant manner, and indeed all TMEM98-associated nanophthalmos pedigrees reported thus far show autosomal dominant inheritance. In the mouse however, ophthalmic phenotypes were only evident in Tmem98A193P/A193P homozygotes.
These findings are consistent with an independent report describing an allelic series of mouse Tmem98 variants, including the chemically-induced p.(Ile135Thr) allele, the engineered human nanophthalmos variants p.(Ala193Pro) and p.(His196Pro), and the tm1a null allele15. A subsequent report revealed that although homozygosity for a null allele of Tmem98 was perinatal lethal in utero, conditional deletion of Tmem98 in retinal pigment epithelium led to the development of larger eyes16.
While homozygosity for a Tmem98 null allele can lead to development of a larger eye in mouse16, compound heterozygosity for a null allele and a nanophthalmos-associated missense variant does not15, suggesting that the nanophthalmos-associated variants are unlikely to be null. Similarly, TMEM98 variants have been positively associated with myopia in genome-wide association studies12,13, suggesting that these variants are likely associated with a larger eye, and therefore more likely to resemble the phenotype of null variants in mouse16.
Although the p.(Ala193Pro) variant in humans was associated with shorter axial lengths, angle closure, ocular hypertension, and disordered scleral collagen, none of these phenotypes were evident in mice heterozygous or homozygous for the same variant. However, the presence of retinal white dots did appear to be shared across species, corresponding histologically to folds in the outer retinal layers in mice, but with no apparent OCT correlates in humans. The clinical significance of these changes is unclear, although they do suggest that retinal phenotypes in human nanophthalmos and posterior microphthalmos may not exclusively be a consequence of a smaller eye, as has been widely suggested, but that TMEM98 may itself play a primary role in retinal integrity.
These findings are consistent with other mouse models of human nanophthalmos, where retinal phenotypes predominate in mouse mutants of PRSS562, MFRP4,5, and MYRF6, the last of which also has intricate functional links with TMEM9816,17. Whether this is a reflection of strain-specific genomic modifiers18, or species-differences in ocular development and anatomy, is unknown.
Materials and methods
For human subjects, patients and family members provided written informed consent. Study protocol were approved by the Southern Adelaide Clinical Human Research Ethics Committee. The study was conducted in accordance with the revised Declaration of Helsinki and followed the National Health and Medical Research Council statement of ethical conduct in research involving humans.
For mouse colonies, CRISPR/Cas9 editing was used to create the Tmem98 c.577G > C p.(Ala193Pro) variant (ENSMUST00000040865.8). This mouse line is designated as C57BL/6J-Tmem98em1Siggs, and abbreviated as either Tmem98wt/wt, Tmem98A193P/wt or Tmem98A193P/A193P mice for wt, heterozygotes and homozygotes respectively. The online tool (http://benchling.com) was used to search for appropriate CRISPR guide sequences. The most appropriate guide was determined by total score, cut site within the target gene, and number of off-target mismatches. The CRISPR guide sequence CCAATCACTGTCTGCCGCTG (forward strand) was determined to be most suitable. A single stranded oligo was used as a donor template to insert the G > C point mutation converting codon 193 from alanine to proline, as well as the silent mutation G > A in codon 194 (to introduce a SapI restriction site 6 bp from the p.(A193P) mutation). The donor template contained 60 bp homology arms either side of the mutations. Cas9 protein was microinjected into C57BL/6J mouse zygotes together with guide RNA and donor oligo, and implanted into pseudopregnant females. Genotyping was initially performed by capillary sequencing of a PCR amplicon (Fwd:CAAAGTTCCCCACTTTCTACAG, Rev:AGGAAGTAGAAGGCTCGCCC). Once germline transmission of the correct variant was confirmed, further genotyping was performed by SapI restriction digest of the same PCR amplicon (wild-type allele 312 bp, mutant allele 154 + 158 bp). Animal ethics approval was obtained from the Flinders University Institutional Biosafety Committee (2015–18) and Animal Welfare Committee (904/16), in accordance with the South Australian Animal Welfare Act (1985) and the Australian Code for the Care and Use of Animals for Scientific Purposes (2013, 8th Edition). Animal use and outcomes were reported in accordance with the ARRIVE guidelines.
Mouse phenotyping included intraocular pressure, measured on non-anaesthetised mice with an Icare TONOLAB rebound tonometer (Icare, Finland), taking the median of three consecutive pressure measurements across a single eye. Prior to retinal imaging, mice were anesthetised with intraperitoneal ketamine (50 mg/kg) and xylazine (5 mg/kg), and eyes dilated with 1% tropicamide and 2.5% phenylephrine drops. Retinal fundus photography was performed after application of lubricant to each eye, overlaid with a glass cover slide. The coverslip neutralised the dioptric power of the cornea-air interface and allowed clear fundus photographs of the retinal dots. Measurements of corneal diameter and globe diameter were performed by a blinded assessor using digital callipers (ImageJ; NIH) on standardised photographs of the anterior segment or enucleated globe, each containing a reference graticule.
Histology was performed on freshly enucleated eyes that were fixed in 4% paraformaldehyde at room temperature for at least 24 h, dehydrated, embedded in paraffin, cut into 5 μm sections, and stained with hematoxylin and eosin.
Electron microscopy was performed by first fixing enucleated eyes in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4). Samples were processed commercially (Electron microscopy division, SA Pathology, South Australia) using a scanning electron microscope. The diameter of collagen fibrils was measured by a blinded assessor using digital callipers (ImageJ; NIH) on 1000 × 1000 pixel regions of interest randomly sampled from 80,000× magnification EM images of cross-sectional collagen fibres. Collagen fibril organisation was subjectively assessed against the human nanophthalmos case EM images by an experienced ocular histopathologist (SK).
All data preparation and analyses were performed using R (version 3.5.1, RCore Team, Austria). Measurements of globe diameter, corneal diameter and collagen fibril diameter were compared between genotypes using a linear model with mixed effects to account for paired eye data. The significance level (alpha) was set at 0.05 for two-tailed hypothesis testing.
Data availability
All data generated or analysed during this study are included in this published article.
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Funding
This study was funded by National Health and Medical Research Council (Grant No. 1116360) and Flinders Foundation (Owen Siggs).
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Conceptualization, O.M.S. and J.E.C.; methodology, M.M.H., M.W., P.Q.T., O.M.S., J.E.C.; software, M.M.H. and O.M.S.; validation, X.X., Y.Y. and Z.Z.; formal analysis, M.M.H. and O.M.S.; investigation, M.M.H., S.J., S.K., M.S.A., S.S., A.Q., P.Q.T., O.M.S., O.M.S. and J.E.C.; resources, J.E.C.; data curation, M.M.H., O.M.S.; writing—original draft preparation, M.M.H., O.M.S.; writing—review and editing, all.; visualization, M.M.H., O.M.S.; supervision, O.M.S.; project administration, O.M.S.; funding acquisition, J.E.C., O.M.S. All authors have read and agreed to the published version of the manuscript.
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Hassall, M.M., Javadiyan, S., Klebe, S. et al. Phenotypic consequences of a nanophthalmos-associated TMEM98 variant in human and mouse. Sci Rep 13, 11017 (2023). https://doi.org/10.1038/s41598-023-37855-x
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DOI: https://doi.org/10.1038/s41598-023-37855-x
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