Abstract
Glioblastoma (GBM) is the most common primary malignant cancer of the central nervous system. Insufficient oxygenation (hypoxia) has been linked to GBM invasion and aggression, leading to poor patient outcomes. Hypoxia induces gene expression for cellular adaptations. However, GBM is characterized by high intertumoral (molecular subtypes) and intratumoral heterogeneity (cell states), and it is not well understood to what extent hypoxia triggers patient-specific gene responses and cellular diversity in GBM. Here, we surveyed eight patient-derived GBM stem cell lines for invasion phenotypes in 3D culture, which identified two GBM lines showing increased invasiveness in response to hypoxia. RNA-seq analysis of the two patient GBM lines revealed a set of shared hypoxia response genes concerning glucose metabolism, angiogenesis, and autophagy, but also a large set of patient-specific hypoxia-induced genes featuring cell migration and anti-inflammation, highlighting intertumoral diversity of hypoxia responses in GBM. We further applied the Shared GBM Hypoxia gene signature to single cell RNA-seq datasets of glioma patients, which showed that hypoxic cells displayed a shift towards mesenchymal-like (MES) and astrocyte-like (AC) states. Interestingly, in response to hypoxia, tumor cells in IDH-mutant gliomas displayed a strong shift to the AC state, whereas tumor cells in IDH-wildtype gliomas mainly shifted to the MES state. This distinct hypoxia response of IDH-mutant gliomas may contribute to its more favorable prognosis. Our transcriptomic studies provide a basis for future approaches to better understand the diversity of hypoxic niches in gliomas.
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Introduction
Glioblastoma (GBM), the most common and malignant high-grade glioma, has been classified by bulk gene expression to exhibit four transcriptional subtypes1,2. Single cell transcriptomics has further revealed intratumoral heterogeneity, with each GBM harboring tumor cells of four distinct differentiation states3. Furthermore, mutant forms of the metabolic enzymes IDH1/2 have been identified as molecular drivers of a subset of gliomas previously known as IDH-mutant GBM, now classified as “Astrocytoma, IDH-mutant”4. Despite these advances in molecular characterization, GBM continues to carry poor prognosis, with no effective treatment available.
A key environmental factor that drives malignant potency of gliomas is hypoxia (insufficient oxygenation), which occurs in specific niches and has been associated with neovascularization, cell migration, metabolic reprogramming, and poor patient survival5,6,7. Hypoxia leads to stabilization of hypoxia inducible factor 1α (HIF1α), a master transcription factor that controls gene programs for metabolic adaptations, as well as cell specific responses8. Part of the hypoxia response is also carried out via a related transcription factor HIF2α, which induces overlapping, but also unique sets of target genes9. HIF-independent transcriptional responses to hypoxia may also be driven by epigenetic changes10.
The distinct hypoxia activated gene programs for the diverse glioma subtypes are not fully resolved, and a better understanding may help reveal new treatment targets for this devastating disease. In this study, we therefore sought to better characterize the diversity of hypoxia responsive gene programs in gliomas. We first utilized a 3D in vitro invasion assay to identify patient-derived GBM stem cell lines of different subtypes that displayed an increased invasive phenotype in response to hypoxia. We then performed RNA sequencing (RNA-seq) to elucidate shared and patient-specific GBM Hypoxia gene signatures, which featured cell migration and immune-suppression.
We next applied the shared GBM Hypoxia gene signature to recent single-cell RNA-seq (scRNA-seq) data of high-grade glioma patients that included 5 IDH-wildtype and 6 IDH-mutant gliomas11. Interestingly, analysis revealed divergent hypoxia responses in IDH-wildtype and IDH-mutant gliomas, with hypoxic glioma cells shifting mainly to a mesenchymal differentiation state in IDH-wildtype, but to an astrocyte-like state in IDH-mutant glioma.
In sum, we reveal patient-specific hypoxia responses in two GBM stem cell lines, and that the mutation status of IDH affects the hypoxia responses in glioma patients. Our findings open the door for further investigations of hypoxia-induced adaptations of glioma cells and of novel targets in the hypoxic subpopulations of gliomas.
Results
Distinct migratory responses of patient GBM lines to hypoxia in 3D invasion assay
We first set out to identify patient GBM lines that would respond to hypoxia by changing their invasive migratory behavior. We utilized a 3D invasion assay to screen eight different patient-derived GBM stem cell lines representing four different transcriptional subtypes from the Human Glioblastoma Cell Culture Resource (Uppsala, Sweden; hgcc.se)12 (Fig. 1A). Importantly, these GBM lines had been established in neural stem cell media, which maintains the pathophysiological characteristics of glioma cells, including invasiveness13,14,15.
The 3D invasion assay was conducted by generating GBM cell aggregates in individual U-wells with 50% Matrigel matrix, cultured either under hypoxic (1% O2) or normoxic conditions for up to 72 h (Fig. 1B). We also applied transient hypoxia by limiting the hypoxia exposure to the first 24 h, followed by 48 h in normoxia. Invasion rates were determined as the ratio of invasion area relative to the area of aggregates at the beginning of the experiment (Fig. 1B).
Out of the eight GBM lines tested, two lines (UCA and UNB) displayed increased invasion under hypoxia when compared to normoxic conditions (Fig. 1D). Interestingly, the patients from whom these two cell lines were derived had the shortest survival (Table S1), suggesting a link of hypoxia-induced invasiveness and GBM malignancy.
Hypoxia induces shared and distinct gene sets in patient-derived GBM lines
To investigate gene expression changes in the two hypoxia-responsive lines (UCA and UNB), we isolated cells from 3D aggregates cultured under normoxia or hypoxia for 72 h, and RNAs were extracted for Illumina RNA sequencing (Fig. 2A).
Principal component analysis (PCA) of the RNA-seq data revealed close clustering of three independent samples of each condition, confirming reproducibility. The transcriptomic profiles showed that the different GBM lines (UCA vs. UNB) were separated along principal component 1 (PC1), consistent with the intertumoral heterogeneity of GBM. For both lines, the hypoxia exposed cells were consistently distinguishable from their normoxic counterparts along PC2 (Fig. 2B).
Among the differentially expressed genes (DEGs) in response to hypoxia (cut offs: P < 0.01 and log2FC > 1) (Table S2–S4), a majority was upregulated in both UCA and UNB lines, consistent with HIF functioning as an “on-switch” (Fig. 2C). Heatmap hierarchical clustering analysis of the DEGs illustrated that majority of the genes induced or suppressed in one cell line were not changed in the other line, underscoring intertumoral heterogeneity of GBM in response to hypoxia (Fig. 2D).
An overlap analysis revealed that among the hypoxia DEGs for UCA and UNB (n = 634 and 986, respectively), 248 were shared, among which 193 were upregulated (Fig. 2E). For subsequent studies, we focused on the 193 shared upregulated DEGs, designated as “Shared GBM Hypoxia DEGs”, which were enriched for gene pathways linked to canonical adaptions to hypoxia, e.g., glycolysis and HIF-1 signaling (Fig. 2F). Among them, four major common themes were discernable: angiogenesis, metabolism (glycolysis), autophagy, and HIF negative feedback loop, exemplified by upregulation of the representative genes VEGFA, SLC2A1, BNIP3, and EGLN3, respectively (Fig. 2G). We further performed qRT-PCR analysis of these four genes in an independent hypoxia assay in UCA and UNB cells, which confirmed their robust induction under hypoxia (Fig. S1).
Hypoxia activates patient-specific gene programs in different gliomas
To elucidate patient-specific transcriptional responses to hypoxia, we next analyzed hypoxia DEGs unique to UCA or UNB cells. For UCA, 389 of the 634 hypoxia DEGs (61%) were unique, among which 249 were upregulated and 137 downregulated (Fig. 3A). For UNB, 738 of the 986 hypoxia DEGs were unique (75%), and among them, 438 were upregulated and 300 downregulated (Fig. 3A).
Pathway analysis of the patient-specific hypoxia DEGs revealed cell migration and anti-inflammation as main biological themes (described below). For instance, for UCA, specific upregulated hypoxia DEGs included MMP2 and STC1, both linked to invasion and progression of gliomas16,17,18, while specific downregulated DEGs included NR1D2, the deficiency of which can promote cancer growth by activating inflammasome19, FRY, which suppresses the growth promoting transcription factor YAP20, and SOX21, which acts as a tumor suppressor of gliomas by inhibiting SOX2 expression and promoting differentiation of glioma cells21. On the other hand, for UNB, specific upregulated DEGs included SMIM3, a leukemia promoter gene, WNT7A, a hypoxia induced EMT driver, PADI2, a tumorigenic breast cancer gene, and RAPGEF4, which modulates gliomas22,23,24,25. Downregulated DEGs in UNB included CXCL3 and FAS, which are related to pro-inflammatory action via chemotaxis of neutrophils and apoptotic pathways, respectively26,27 (Fig. 3A).
Ingenuity Pathway Analysis (IPA) core enrichment investigation of the UCA-specific hypoxia DEGs also highlighted activation of Tissue Factor pathway, which plays a role in tumor progression and angiogenesis28 and has been linked with remodeling of the extracellular matrix and microenvironment of GBM. Enriched pathways also included inhibitor of differentiation 1 (ID1) signaling, which is linked to glioma invasion and suppression of differentiation of GBM stem cells29, CDK5 signaling, a well-established regulator of neural progenitor migration30 and associated with GBM invasion31, and CXCR4 signaling, which emphasizes a migratory response of glioma cells to hypoxia32 (Fig. 3B).
We further used the Graphical Summary platform of IPA for patient-specific hypoxia DEGs to construct an overview of predicted relationships among transcription factors, cytokines, and other functional pathways (Fig. 3C). Interestingly, the network of UCA-specific hypoxia DEGs featured the transcription factor JUN as one of its central nodes with links to the HIF1A and VEGFA nodes. JUN is known to drive malignant properties of glioma cells33. Other nodes included WNT3A and the canonical WNT-signaling transcription factor β-catenin (CTNNB1). Interestingly, glioma derived WNT3A has been reported to trigger an anti-inflammatory program in microglia, resulting in a less robust and more suppressed immune cell population34.
Core IPA analysis of UNB-specific hypoxia DEGs showcased enrichment of the GABA receptor signaling, which has been associated with suppressed proliferation, tumor quiescence, and increased therapy resistance35. In addition, there was enrichment for pathways with predicted inhibition of the Neuroinflammation Signaling Pathway and Interferon Signaling, suggesting an immunosuppressive reaction of UNB under hypoxia (Fig. 3D).
The IPA Graphical Summary of UNB-specific hypoxia DEGs highlighted overall downregulation/inhibition of pathways (Fig. 3E), in contrast to the mainly activated pathways of UCA-specific DEGs (see Fig. 3C). This included predicted inhibition of Neuroinflammation Signaling Pathway, movement of phagocytes, and lymphocyte migration. This was paralleled by downregulation/inhibition of nodes for the cytokines and cytokine related proteins IRF7, TNF, MYD88, IL1A and IL1B, which are immune regulators of pro-inflammatory pathways related to interleukin-1 and Toll-like receptor signaling36,37,38,39.
IDH mutation status influences the hypoxia response of GBM cells
To better understand the clinical relevance of the Shared Hypoxia DEGs of UCA and UNB (193 upregulated DEGs), we applied this hypoxia signature across glioma cells in 11 high-grade glioma patients, including 5 IDH-wildtype and 6 IDH-mutant gliomas based on a single cell RNA-seq dataset11. For each glioma cell, a signature score for the Shared GBM Hypoxia DEGs was calculated (Fig. 4A). By using the proportion of cells expressing VEGFA as a guide for hypoxia induction, we selected the Shared Hypoxia signature score of 0.25 as a threshold to separate cells into hypoxic versus non-hypoxic, with about 3.8% of GBM cells (1170 out of a total of 30,544) classified as hypoxic (Fig. 4B,C).
To determine the impact of hypoxia on cellular differentiation states (as defined by Neftel and colleagues3), we first analyzed the different cell states in each glioma patient (Fig. S2) and then asked if the cell states would be differently represented in hypoxic versus non-hypoxic glioma cells. Interestingly, we found a higher representation of astrocyte-like (AC) and mesenchymal-like (MES) states in hypoxic population, from 20.9 to 25.8% for AC and from 24.9 to 47.8% for MES state, suggesting that hypoxia drives a shift towards AC and MES states. In contrast, the proportion of oligodendrocyte precursor-like (OPC) and neural progenitor-like (NPC) states decreased in hypoxia, from 16.5 to 7.2% for OPC, and from 37.6 to 19.0% for NPC, respectively (Fig. 4D).
We next examined the shift of cell states on the level of individual glioma patients according to IDHstatus, as gliomas with IDH1/2 mutation have been defined as a separate entity, with better prognosis among high grade gliomas40,41. The relative proportion of the hypoxic cell population was comparable among glioma patients, independent of IDH mutation status, ranging from 1.7 to 5.5% (Fig. 5A). Intriguingly, for IDH-wildtype glioma patients, we observed in all cases a distinct shift of hypoxic cells towards MES state (Fig. 5B), whereas among the IDH-mutant gliomas, the MES shift was largely absent; instead, we detected a strong shift towards an AC state in response to hypoxia (Fig. 5B). As the MES state is associated with higher malignancy and shorter survival1, the divergent hypoxia response of IDH-mutant gliomas towards AC state may be part of the molecular underpinnings of their better prognosis.
Shared GBM Hypoxia DEGs signature is an alternative gene set for analysis of GBM hypoxia
For further confirmation of theShared GBM Hypoxia signature (193 upregulated DEGs), we performed a comparison to the Hallmark Hypoxia signature from the Molecular Signatures Database (MSigDB) (200 genes, compiled from multiple studies42). Interestingly, only 20% of the genes from the two sets overlapped (Fig. S3A), indicating again divergent hypoxia gene responses in different cell types. We also applied the MSigDB Hallmark Hypoxia signature to the Johnson et al. patient dataset to separate glioma cells into non-hypoxic and hypoxic cells (Fig. S3B). Analysis of the cell states again confirmed that hypoxia was associated with an enrichment of AC or MES states, with a higher shift of IDH-mutant gliomas to the AC state (Fig. S3C,D).
We also made comparison to a recently defined cancer wide hypoxia signature (HIF metagene) based on genome-wide HIF binding and transcriptome profiling of several cancer cell lines (representing lung, colorectal, liver, prostate, kidney, and breast cancer) under hypoxia43. We found that more than half of the HIF metagene signature (26/48) overlapped with our GBM Shared Hypoxia signature (Fig. S4A). We applied the HIF metagene signature to the glioma patient dataset, using a threshold that yielded ~ 3.6% of hypoxic GBM cells, a similar proportion as our Shared GBM Hypoxia signature (Fig. S4B). Analysis of the cell states showed consistent results as above: an overrepresentation of the MES state among hypoxic glioma cells (Fig. S4C). However, an increase in AC state using the HIF metagene was not observed, illustrating our hypoxia signatures unique to glioma (Fig. S4C). When analyzing glioma patients stratified by IDH status, analysis with the HIF metagene consistently identified a significant increase towards the MES state among hypoxic cells of IDH-wildtype patients, and a trend of AC shift among IDH-mutant patients (Fig. S4D). Together, these comparative analyses confirmed that the Shared GBM Hypoxia DEGs signature identified in our study is a valuable alternative for the identification and analysis of hypoxic cells in gliomas.
Discussion
Glioblastoma is an aggressive brain cancer with high inter-and intratumoral heterogeneity. In this study, we investigated the role of hypoxia as an environmental factor promoting invasiveness and cellular diversity of gliomas. We showed that hypoxia induced both shared and patient-specific gene programs in patient-derived GBM stem cells. While the shared gene set mainly concerned canonical adaptations to hypoxia such as angiogenesis and glycolysis, patient-specific hypoxia genes featured cell migration or immunosuppressive pathways. By applying the Shared GBM Hypoxia signature to a larger single cell RNA-seq patient dataset, we revealed that IDH mutation status influences the responses of glioma cells to hypoxia, with IDH-wildtype glioma mainly shifting to a mesenchymal-like cell state, whereas IDH-mutant gliomas shifting more towards to an astrocyte-like state.
The 3D invasion assay with patient-derived GBM stem cell lines demonstrated that only two out of eight lines, UCA and UNB, displayed a hypoxia-induced increased migratory response. Interestingly, the two patients from whom UCA and UNB were derived had the shortest survival times among the eight patients. Thus, increased migration may not be a general hypoxia response in all glioma patients, but restricted to certain subtypes with more aggressive features. Our bulk RNA-seq analysis showed a clear induction of migration-related genes for the UCA GBM line in response to hypoxia, while immune suppressive pathways were prominent for UNB. Further investigations are needed to reveal the functional significance of these distinct hypoxia-induced gene programs for invasion and the GBM immune landscape.
Our study applied severe hypoxia (1%) to the GBM cells, which triggers a response in which the master transcription factor HIF-1α plays a major role. It should be noted that mild chronic hypoxia (1–5% O2) can lead to a switch of the hypoxia responses to one carried out by HIF-2α with potentially divergent functions44. Future studies with the 3D aggregate system under mild hypoxia conditions will be informative to investigate a potential HIF1/2 switch in invasive GBM cells.
The shift of hypoxic cells in IDH-wildtype gliomas to a more MES-like state is in alignment with the study by Neftel et al. that characterized the MES-like states of glioma cells as hypoxia dependent3. Interestingly, the preferential shift of hypoxic cells to an AC-like state in IDH-mutant gliomas may partly account for the more favorable prognosis of this glioma subtype, and investigations are warranted to elucidate the molecular underpinnings of this difference.
One limitation of the current study is that only two patient-derived GBM lines were sequenced, and additional sequencing of patient samples of diverse GBM molecular subtypes, in particular, sequencing of the six remaining GBM cells lines that did not show increased invasiveness in response to hypoxia would help to compare hypoxia-invasive versus non-invasive glioma transcriptome signatures. Additional information could also be gained by applying 3D tomographic imaging to the GBM aggregates45 and application of proliferations markers46, which could help to determine if increased GBM invasiveness would go along with reduced proliferation, as has been proposed in the “go or growth” theory47. In this context, it is noteworthy that glioblastoma cells do not necessarily follow such dichotomy, as recent studies have demonstrated that invasive GBM cells are often highly proliferative48. A further limitation of our study is that invasion assays were conducted in Matrigel, a laminin-rich matrix that does not fully represent the brain extracellular matrix, which is rich in glycosaminoglycans like hyaluronic acid (HA)49. Future studies in HA-based 3D matrices (see for example ref.50) will present a closer match with the actual matrix conditions of the brain.
Furthermore, the interactions of hypoxic glioma cells with the immune cell environment will need further exploration. This is particularly important in light of the fact that tumor-associated microglia and macrophages (TAMs) can comprise > 30% of the tumor bulk in GBM and contribute to tumor progression and treatment resistance51. The distribution of TAMs varies between molecular subtypes of GBM, with the highest TAM content found in GBM of mesenchymal subtype (associated with worst prognosis), and medium and low content in classical and proneural subtypes, respectively52. Thus, immune interactions, as suggested by the transcriptomic response of UNB GBM cells to hypoxia, may be a key factor in how hypoxia relates to GBM malignancy. In this context it is noteworthy that our teams have recently characterized the in vivo hypoxia response in a murine GBM model, which featured attraction and sequestration of immune cells inside of hypoxic zones with immunosuppressive gene signatures53, a mechanism potentially applicable in human GBM patients.
In sum, our study provides new insights into the molecular strategies that glioma cells exploit to adapt to hypoxia, a key factor of the tumor microenvironment and a driving force of cellular diversity in glioma. Targeting the hypoxia-driven pathways may improve therapies for GBM patients.
Methods
GBM stem cell lines
The human GBM stem cell lines UPA (U3047), UPB (U3082), UNA (U3021), UNB (U3085), UMA (U3035), UMB (U3065), UCA (U3056), and UCB (U3086) (all IDH-wildtype) were obtained from the Human Glioblastoma Cell Culture Resource (hgcc.se) of Uppsala University, Sweden, where the cell lines had been established from resected grade 4 GBMs12. See Table S1 for additional HGCC cell line information on subtype, gender, age, and survival time. The cell lines were de-identified and no protected patient data was transmitted by HGCC to investigator team. Of note, transcriptional classification of GBM has been updated in 2020 to 3 molecular subtypes1, but at the time that the lines were characterized at HGCC four transcriptional subtypes were recognized2.
The GBM lines were maintained in neural stem cell media as adherent cultures on laminin-coated dishes, which preserves main physiological characteristics GBM tumor biology13,14,15. Tissue culture dishes were coated with laminin (Invitrogen, 10 µg/ml in PBS) for 1 h at 37 °C. Plates were washed 3 times with PBS before adding human neural stem cell (HNS) media, containing Neurocult NS-A basal medium, Neurocult human NS-A proliferation supplement (Stemcell Technologies), 0.0002% heparin, 10 ng/ml bFGF (Peprotech), and 20 ng/ml EGF (Peprotech). Cells were passaged by dissociation with Accutase (Gibco), incubated for 3 min in 37 °C incubator before dissociation with a micropipette. Two volumes of basal NS-A media were added to dilute Accutase, and cells were pelleted by centrifugation at 1200 rpm in a tabletop centrifuge (Eppendorf Centrifuge 5702) for 3 min, and then resuspended in HNS media before plating.
3D invasion assay
We followed the protocol for 3D invasion assay of tumor aggregates as described54,55. Briefly, GBM cells were seeded in 96 well low attachment U-shaped bottom plates (Corning) at 2000 cells/well in 100 µl of HNS media. Cells were incubated for 3 days in a cell culture incubator at 37 °C to let them form spheres at the bottom of wells. Next, 50 µl of HNS media was replaced in each well with ice-cold Matrigel (Corning) or the equivalent product Cultrex basement membrane extract (R&D Systems), and after further incubation for 4 h, the gel had solidified (defined as t = 0 h). Photos were taken with a cell culture microscope (Olympus CKX53) at t = 0 h, as well as at t = 24, 48, and 72 h. The perimeter of aggregates at each time point was measured as a ratio to the perimeter of the aggregate in the same well at t = 0 h.
RNA bulk sequencing
To isolate RNA from cultured GBM aggregates, the solid Matrigel was dissolved by addition of Cultrex Organoid Harvesting Solution (OHS; R&D systems). For each condition, 30 GBM aggregates were collected into a 6-well dish with a 1 ml micropipette with cut off tip from the 96-well U-wells. A volume of 5 ml Cultrex OHS was added and plates were placed for 30 min on a shaker in a 4 °C cold room. Aggregates were collected in a 15 ml tube and pelleted by centrifugation at 1000 rpm for 5 min, then 350 µl RLT buffer of the RNAeasy kit (Qiagen) was added to lyse aggregates by grinding them up with a Dounce homogenizer. The lysed RNA in RLT buffer was stored at − 80 °C until processing.
To prepare cDNA libraries for Illumina sequencing from lysed RNA of GBM aggregates, total RNA was isolated with the RNeasy Micro kit. The cDNA libraries were synthesized with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina by using 100 ng RNA as input for each replicate sample. Libraries were sequenced at John Wayne Cancer Institute, Santa Monica, on an Illumina HiSeq2500 device with 75 bases single-end reads for a coverage of about 30 million reads per sample.
Bulk RNA-seq bioinformatic analysis
Raw sequencing reads from UCA and UNB cell lines were mapped to hg38 human genome using HISAT256. Counts of reads mapping to genes were obtained using featureCounts software of Subread package against Ensembl v90 annotation57. Differential expression analysis was done using the DESeq2 R package58. Differentially expressed genes (DEGs) were defined as genes with log2 fold-change (FC) > 1 between conditions and adjusted P value < 0.01. Enrichment analysis of gene sets for biological pathways was performed with the Enrichr platform (https://maayanlab.cloud/Enrichr/)59 and the Ingenuity Pathway Analysis (IPA) tool (Qiagen)60. The combined score chosen to rank pathways is the multiplication of the odds ratio by the negative natural log of the P value of enrichment.
qRT-PCR of hypoxia induced genes in GBM stem cell lines
UCA and UNB GBM stem cell lines, maintained on laminin-coated dishes, were exposed for 16 h to hypoxic (1% O2, hypoxic chamber) or normoxic control (standard cell culture incubator) conditions. Total RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen) and quantified using a Nanodrop device (Thermo Scientific). cDNA was prepared using a Superscript III cDNA synthesis kit (Invitrogen) according to manufacturer’s protocol. Finally, the cDNAs were analyzed for expression levels of hypoxia-induced genes by qRT-PCR on an ABI Prism 7900HT Sequence Detection instrument using PerfeCTa SYBR Green FastMix (Quantabio). Expression of the house keeping gene HPRT1 was used for normalization of expression levels in each sample.
qRT-PCR primers:
HPRT1-F2: AGATGGTCAAGGTCGCAAG
HPRT1-R2: GTATTCATTATAGTCAAGGGCATATCC
VEGFA-F1: AGTCCAACATCACCATGCAG
VEGFA-R1: TTCCCTTTCCTCGAACTGATTT
SLC2A1-F1: AAAGTGACAAGACACCCGAG
SLC2A1-R1: TGTCAGGTTTGGAAGTCTCATC
BNIP3-F1: GTTCCAGCCTCGGTTTCTATT
BNIP3-R1: AGCCCTGTTGGTATCTTGTG
EGLN3-F1: ATTCATAGCAGATGTGGAGCC
EGLN3-R1: TCAGCATCAAAGTACCAGACAG
Bioinformatic analysis of glioma patient data
The glioma patient single cell data of the Johnson et al. study11 was analyzed using the Seurat package (v4.3.0) as previously described61. Briefly, we loaded the Johnson et al. study11 count matrix using the read10x function, subset on the glioma cells, and removed low quality cells with fewer than 1000 unique molecular identifiers (UMIs), fewer than 400 detected genes or greater than 25% mitochondrial genes from downstream analysis. Remaining cells were ‘LogNormalized’ using NormalizeData function, and 2000 variable genes were identified using the default ‘vst’ method in the ‘FindVariableFeatures’ function. The normalized data was scaled using the ScaleData function, which was then used to perform principal component (PCA) analysis on the variable gene expression space. Following data integration, Uniform Manifold Approximation and Projection (UMAP) on the first 20 PCAs was performed for visualization of the glioma scRNA-seq data. We then used the AddModuleScore function with default parameters to calculate the enrichment of different hypoxia gene signatures in individual glioma cells.
VEGFA+ cells were defined as cells with 2 or more VEGFA unique molecular identifier (UMI) counts. We applied a threshold score of 0.25 for the signature Shared GBM Hypoxia DEGs module (defined in this study; see Table S2 and S5) to separate cells into hypoxic (> 0.25) and non-hypoxic (≤ 0.25) classes. The Lombardi et al. study pan cancer hypoxia gene signature was similarly analyzed on the Johnson et al. scRNA-seq data for comparative analysis. We applied a threshold score of 0.55 for the cancer wide hypoxia signature (HIF metagene) as defined by Lombardi et al. (Table S5) to separate cells into hypoxic (> 0.55) and non-hypoxic (≤ 0.55) classes in similar proportions as classified using the Shared GBM Hypoxia DEGs signature.
Comparative analysis for cell states were visualized by quadrant graphs calculated as described in Neftel et al.3. Briefly, scores for each state were calculated using the Seurat AddModuleScore function on gene signatures for MES1/2, AC, OPC, and NPC1/2 states, as defined by Neftel et al.3 (Table S5). The cell state with the highest score determined the categorization of the cell (cells in MES1 or MES2 state were summarized as MES, and cells in NPC1 or NPC2 state as NPC).
Statistical analysis
Statistical analysis of GBM aggregate invasion was performed with GraphPad Prism 9. One-way ANOVA with Tukey correction was applied for comparison of aggregate growth for each time point. Wilcoxon rank-sum test was used for analysis of cell state proportions between patient groups. The GraphPad Prism setting NEJM (New England Journal of Medicine) for reporting of P values was applied. P ≤ 0.05 was considered as statistically significant (*); P ≤ 0.01, **; P ≤ 0.001, ***.
Data availability
The RNA-Seq data has been deposited at the NCBI Gene Expression Omnibus (GEO) database under accession number GSE232725.
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Acknowledgements
We thank the Uppsala GBM cell culture resource (hgcc.se) for providing us with GBM patient lines. We are grateful to Dalia Halawani and other members of the Zou and Friedel labs for scientific advice. This work was supported by National Institutes of Health grants F31NS124259 (to V.M.), Icahn School of Medicine at Mount Sinai seed funding (to A.M.T.), R01NS107462 (to H.Z. and R.H.F.), R21NS125700 (to H.Z. and R.H.F.), and R01NS092735 (to R.H.F.).
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V.J.M., H.Z., and R.H.F. conceived and designed the study. V.J.M. performed bulk transcriptomic studies of glioma cell aggregates. R.T., R.R., and L.St. performed glioma cell invasion assays. S.N., A.S., and Y.H. contributed cell lines and qRT-PCR expression analyses. A.W. and S.K. performed whole-transcriptome RNA sequencing. A.R. and L.Sh. performed bioinformatic analyses of bulk whole transcriptome data. A.M.T conceived and supervised all single-cell RNA-seq data analyses carried out by M.E.U. Manuscript and figures were prepared by V.J.M., M.E.U., H.Z., A.M.T., and R.H.F.
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Marallano, V.J., Ughetta, M.E., Tejero, R. et al. Hypoxia drives shared and distinct transcriptomic changes in two invasive glioma stem cell lines. Sci Rep 14, 7246 (2024). https://doi.org/10.1038/s41598-024-56102-5
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DOI: https://doi.org/10.1038/s41598-024-56102-5
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