Abstract
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors. However, the mechanisms underlying ESCC tumorigenesis have not been fully elucidated. Thus, we aimed to determine the key genes involved in ESCC tumorigenesis. The following bioinformatics analyses were performed: identification of differentially expressed genes (DEGs); gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis; integrated analysis of the protein–protein interaction network and Gene Expression Profiling Interactive Analysis database for validation of hub genes. Finally, western blotting and qPCR were used to explore the expression of cell division cycle 6 (CDC6) in ESCC cell lines. Immunohistochemistry analysis of ESCC samples from patients and matched clinical characteristics was used to determine the effects of CDC6. A total of 494 DEGs were identified, and functional enrichment was mainly focused on cell cycle and DNA replication. Biological pathway analysis of the hub genes was closely related to the cell cycle. We found that CDC6 was upregulated in ESCC cell lines and patient tissues and was related to the clinicopathological characteristics of ESCC. In conclusion, this study identified hub genes and crucial biological pathways related to ESCC tumorigenesis and integrated analyses indicated that CDC6 may be a novel diagnostic and therapeutic target for ESCC.
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Introduction
Esophageal carcinoma (EC) is one of the most malignant tumors with a low 5-year survival rate and is the sixth leading cause of cancer death worldwide1. The two most common EC histological subtypes are esophageal adenocarcinoma (EAC) and esophageal squamous cell carcinoma (ESCC). ESCC accounts for 90% of all EC cases and is the predominant histological type in Eastern Asia2. New developments have been made in the multimodal treatment of several cancers; however, EC is still associated with a poor prognosis and high mortality rate. In most cases, EC is diagnosed at an advanced stage, disqualifying patients from surgical resection. Additionally, owing to chemotherapy resistance, EC recurrence is associated with poorer prognosis and shorter survival3. Therefore, identifying new biomarkers and targets for patients with ESCC is crucial.
The tumorigenesis of ESCC is related to genetic and environmental factors as well as individual lifestyle factors, including smoking and the consumption of hot drinks4. Gene mutations promote ESCC carcinogenesis via complex biological processes and multiple molecular interactions. For example, MTA1, SOX2, and FXYD-3 upregulation is associated with proliferation and angiogenesis in ESCC5,6,7. With the use of next-generation gene sequencing, novel genes and biomarkers have been identified8. Numerous differentially expressed genes (DEGs), critical for ESCC progression, have been reported9,10,11. However, the underlying mechanisms of ESCC tumorigenesis remain elusive and only a few hub genes are identified as ESCC biomarkers12.
Therefore, in this study, we aimed to identify DEGs and hub genes, and the underlying molecular mechanisms that contribute to ESCC progression through bioinformatics analyses of gene expression profiles obtained from four GEO databases.
Results
Identification of common DEGs
Gene expression data was obtained from four GEO datasets (GSE23400, GSE38129, GSE20347, and GSE29001). Using differential gene expression and Venn analyses, we identified 494 overlapping DEGs. Of the 494 genes, 348 were upregulated, and 146 were downregulated (Fig. 1A,B; Table 1).
Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses of the common DEGs
We attempted to determine the roles of the identified DEGs in the progression of ESCC. Functional and pathway enrichment analyses were performed using Database for Annotation, Visualization, and Integrated Discovery (DAVID) software. GO analysis revealed that the biological processes of the DEGs (Supplementary Material 1) were significantly enriched in extracellular matrix organization, mitotic cell cycle phase transition, nuclear division, DNA replication, and chromosome segregation. Enriched cell components of the DEGs were primarily involved in the extracellular matrix, chromosomal region, spindle, nuclear chromosome, and endoplasmic reticulum lumen. Changes in molecular function were mainly enriched in extracellular matrix structural constituents, structural molecule activity, DNA replication origin binding, and DNA helicase activity (Fig. 1C). KEGG pathway analysis of the upregulated genes (Supplementary Material 2) revealed involvement in the cell cycle, DNA replication, protein digestion and absorption, extracellular matrix (ECM)–receptor interaction, small cell lung cancer, amoebiasis, p53 signaling pathway, cancer pathway, IL-17 signaling pathway, and AGE-RAGE signaling pathway in diabetic complications13,14,15,16(Fig. 1D).
Protein–protein interaction (PPI) network and module analysis
The PPI network of the DEGs was constructed using the STRING database and Cytoscape software. The top three significant modules (Supplementary Material 3) were selected using the MCODE plug-in in Cytoscape (Module 1: MCODE score = 66.056; Module 2: MCODE score = 21.758; Module 3: MCODE score = 8.621) (Fig. 2A). We then analyzed the functions of these modules. The enriched KEGG pathway showed that Module 1 consisted of 73 nodes and 2378 edges mainly enriched in the cell cycle, DNA replication, and oocyte meiosis. Module 2, containing 34 nodes and 359 edges, was primarily correlated with protein digestion and absorption by the ECM receptor. Module 3, which included 30 nodes and 125 edges, primarily correlated with cytokine–cytokine receptor interactions, chemokine signaling pathways, and IL-17 signaling pathways13,14,15,16 (Fig. 2B).
Expression level of hub genes
Based on the degree of connectivity, the top 10 significant hub genes were filtered (Table 2). We created a heatmap of the 10 most essential hub genes, based on four data series (GSE23400, GSE38129, GSE20347, and GSE29001) and found that the hub genes could distinguish ESCC samples from normal samples (Fig. 3A). The PPI network of the 10 hub genes was predicted using the STRING database and constructed using Cytoscape (Fig. 3B). The biological pathways for the top 10 hub genes were cell cycle, mitosis, cell cycle checkpoints, G2/M checkpoints, G0 and Early G1, DNA replication, and Mitotic G1-G1-/S phases (Fig. 3C). Furthermore, we constructed a network of hub and co-expressed genes (Fig. 3D) and performed gene co-expression analysis of the hub genes using the STRING database and FunRich software (version 3.1.3; http://www.funrich.org/), which indicated that the hub genes may strongly interact with each other (Fig. 3E).
Expression verification of hub genes and genetic alteration
To investigate the transcriptional expression difference of the hub genes between tumor and normal tissues, we analyzed the datasets from the Gene Expression Profiling Interactive Analysis (GEPIA) database using one-way analysis of variance (ANOVA) (cut-off criteria: |log2FC|= 1 and p = 0.01). As shown in Fig. 4A, compared with normal tissues, the genes were significantly upregulated in tumor tissues. We then investigated the genetic alterations in the hub genes, and found that RFC4 and CDC6 had the top two frequencies of alteration (35% and 4%, respectively), including missense mutations, truncation mutations, amplifications, and deep deletions (Fig. 4B). These results suggest that RFC4 and CDC6 may play roles in the development and progression of ESCC and remained of great significance to further research.
Receiver operating characteristic (ROC) analysis of hub genes and drug–hub gene interactions
ROC curve analysis was performed to assess the diagnostic value of the top ten hub genes for ESCC (Fig. 5A). The expression levels of hub genes were extracted from four databases (GSE23400, GSE38129, GSE20347, and GSE29001). The area under the curve (AUC) of these genes ranged from 0.8353 to 1.000. Furthermore, we identified promising drugs associated with these hub genes. By searching druggable gene categories in DGIdb, we found that most hub genes matched with tumor suppressor genes (Table 3). Finally, 35 Food and Drug Administration (FDA) approved drugs (Table 4) targeting CDK1, TOP2A, AURKA, and PCNA were identified, with a large majority of the drugs inhibiting TOP2A. Furthermore, a downstream network of the 10 hub genes was constructed using the STITCH database (Fig. 5B). These modules showed that the hub genes were mainly associated with CDK, CDC, and MCM family members, and drugs including doxorubicin, rapamycin, etoposide, paclitaxel, amsacrine, levofloxacin, alisertib, MLN8054, aminopurvalano, dexrazoxane, MgATP, and MgADP.
High expression levels of CDC6 and its clinicopathologic characteristics in ESCC
Through extensive literature review, we found that RFC4, with the highest mutation frequency, has been reported in ESCC, whereas few studies have focused on CDC6 in ESCC. To determine whether CDC6 plays a crucial role in ESCC, we evaluated the expression levels of CDC6 in ESCC cell lines. Both qPCR and western blotting showed that, compared to that in human esophageal epithelial cells, CDC6 was upregulated in ESCC cell lines (Fig. 6A,B). Furthermore, to detect the expression of CDC6 in ESCC samples, we performed immunohistochemical staining. Of the 35 samples, 26 exhibited high CDC6 expression levels, while the remaining samples showed low CDC6 expression (Fig. 6C,D). Statistical analysis of the H-score showed that, compared to normal esophageal tissues, CDC6 was upregulated in ESCC tissues (Fig. 6E). Therefore, we aimed to determine the relationship between CDC6 and the clinicopathological characteristics of ESCC using CDC6 H-scores (Supplementary Material 4). We selected five terms: age, grade, tumor size, lymph node metastasis, and stage. We found that the gene expression level of CDC6 was related to tumor size, age, lymph node metastasis, and stage, whereas it was not associated with tumor grade (Fig. 6F,G).
Discussion
Although many studies have focused on ESCC tumorigenesis17, the incidence of ESCC remains high and its prognosis poor. Therefore, in this study, using expression data obtained from four datasets, we aimed to identify the critical genes and biological pathways involved in ESCC tumorgenesis. We identified 490 DEGs, and GO enrichment analysis showed that the identified DEGs were mainly enriched in the mitotic cell cycle phase transition, DNA replication origin binding, and biological pathways primarily enriched in the cell cycle, DNA replication, and protein digestion and absorption, indicating that the DEGs may be associated with the progression of ESCC.
We constructed a PPI network and selected the top three valuable modules. The biological pathway analyses of Module 1 revealed that the modules were mainly related to cell cycle and DNA replication, indicating that the genes may participate in the regulation of ESCC proliferation. Deregulation in proliferation remains is key to tumor development18. We found that part of Module 1 is related to the cell cycle and DNA replication, and previously, researchers have found that RFC4 is associated with increased DNA copy number alterations in ESCC19. CDK1, CCNB1, and CCNB2 are related to the cell cycle and are altered in ESCC20. Matrix metalloproteinases (MMPs) and collagen family members constitute the majority of Module 2. MMPs are involved in tumor invasion and metastasis. For instance, MMP13 participates in the proliferation and invasion of ESCC21. MMP9 can degrade Collagen IV in the basement membrane, which could promote invasion22. The ectopic expression of the collagen family is associated with the prognosis of some cancers23,24,25,26. The collagen family is associated with invasion and migration, which participate in epithelial to mesenchymal transition (EMT) and extracellular matrix remodeling27. Several genes of the collagen family have been identified as potential diagnostic and prognostic biomarkers for ESCC; however, the underlying molecular mechanisms need to be further expored28,29. The PPI network of Module 3 was mainly connected to the chemokine signaling pathway, cytokine–cytokine receptor interaction, and the IL-17 signaling pathway. Chemokine signaling pathways cause differences in the proliferation, angiogenesis, EMT, and metastasis of various cancers. CXCL16/CXCR6 chemokine signaling mediates breast cancer30. IL-8 and CXCR-1 are involved in the EMT in colon carcinoma31. GROalpha-CXCR2 and GRObeta-CXCR2 signaling play crucial roles in ESCC cell proliferation32.
Based on the degree of connectivity, we considered the top 10 genes as hub genes. Both the heatmap and the results from the GEIPA database showed that the expression levels of the genes were significantly higher in tumor samples than in normal tissues, indicating that the genes may play key a role in the development of ESCC. The biological pathways of the 10 hub genes were mainly associated with cell cycle transition, indicating that the genes may affect the proliferation of tumor cells. Cyclin B1(CCNB1) silencing inhibited cell proliferation and facilitated senescence in pancreatic cancer33. CCNB1 is involved in the pathogenesis of esophagus carcinoma, and the CCNB1 upregulation is associated with a poor prognosis in patients with ESCC34. PCNA serves as a moving platform that allows DNA- and chromatin-interacting proteins to operate at the fork in a DNA sequence-independent manner, and targeting PCNA-1 can inhibit the proliferation of lung cancer cells28,35,36. Thus, the functions of the genes correspond to their respective biological pathways. Additionally, we constructed an interactive network including the 10 hub genes and the top 60 frequently altered neighboring genes in ESCC. Several significant genes, such as TP53, EGFR, and PARP137,38 had direct or indirect connections to the 10 hub genes. These interactions indicate that hub genes may participate in ESCC tumorigenesis. The molecular mechanisms underlying these correlations warrant further study.
Using the hub genes, we searched for candidate drugs and 35 drugs with therapeutic efficacy against ESCC were identified. Four of the 10 hub genes, CDK1, TOP2A, AURKA, and PCNA, may be the potential drug targets. Notably, TOP2A is a promising target for ESCC20. In addition to doxorubicin, rapamycin, paclitaxel, and etoposide, we identified new drugs, such as levofloxacin, dexrazoxane, and amsacrine, that have not been used in ESCC. Further studies and clinical trials are required to explore their effects in ESCC. Most of the hub genes interacted with MgATP, MgADP, and phosphate. Intracellular ATP is critical for chemoresistance in colon cancer cells39 and the effect of drug–gene interactions on ESCC chemoresistance requires further research.
Alterations in hub genes included missense mutations, truncating mutations, amplification, and deep deletions. RFC4, with the highest mutation frequency, was upregulated in the early stage and was associated with early nodal metastases and tumor immunity in ESCC19. CDC6, with the second highest alteration frequency, has rarely been reported in ESCC. CDC6 forms part of the pre-replication complex that controls DNA replication licensing in the cell cycle40. Previous studies have shown that CDC6 was overexpressed in other tumors41,42,43. The expression level of CDC6 in ovarian cancer tissues was significantly higher than that in adjacent tissues and it was related to tumor stage, differentiation degree, lymph node metastasis, ascites and prognosis, which was an independent factor of ovarian cancer patients44. Mahadevappa et al. suggested that CDC6 expression was significantly increased in breast cancer tissues and correlated with poor prognosis of patients45. Further studies showed that down-regulation of CDC6 expression in bladder cancer could significantly inhibit a variety of malignant phenotypes of tumor cells46. After knocking out CDC6, the proliferation of tongue squamous cell carcinoma cells was inhibited47.
Additionally, CDC6 upregulation represses E-cadherin correlates with EMT48. CDC6 is also involved in the progression of ESCC induced by RFBP-and circular RNA circNELL249,50. Consistent with the results from the bioinformatics analysis, CDC6 was upregulated in the ESCC cell lines. Furthermore, we explored the clinical significance of the findings by analyzing the CDC6 H-scores. The tumor samples had higher H-scores than normal samples. CDC6 expression was associated with tumor size, lymph node metastasis, and disease stage, indicating that CDC6 may promote the proliferation and invasion of tumor cells and serves as a novel ESCC therapeutic target. This study has some limitations. First, the number of clinical samples used to investigate the expression level of CDC6 was relatively small. Additionally, the effect of CDC6 on the prognosis of patients with ESCC remains unclear. The molecular mechanisms and biological effects of CDC6 in ESCC have not been fully elucidated.
Conclusions
Using comprehensive bioinformatics analysis and in vitro assays, we demonstrated that the CDC6 gene plays a key role in the progression of ESCC and serves as a novel potential biomarker and therapeutic target for ESCC.
Methods
Data collection and DEGs extraction
Gene expression profiles of ESCC and adjacent normal tissues (GSE23400, GSE38129, GSE20347, and GSE29001) were downloaded from the GEO database (http://www.ncbi.nlm.nih.gov/geo/). The GSE23400 dataset consisted of 53 pairs of tumor tissues and matched noncancerous samples. GSE38129 consisted of 30 pairs of tumor and normal tissue samples. GSE20347 consisted of 17 pairs of tumor and normal esophageal tissue samples. GSE29001 consisted of 21 tumor samples and 24 normal tissues. DEGs were identified using R, and the significance procedures were as follows: the Affy package was used to perform background corrections and normalize the data, and then the Limma package was used to perform differential expression analysis. p < 0.05 and logFC (fold change) > 1 were set as cut-off criteria. Volcano plots for each dataset was drawn in R using the ggplot2 package, and the overlapping DEGs among the four microarrays were visualized using the Venn diagram tool (https://bioinfogp.cnb.csic.es/tools/venny/).
Functional enrichment analyses
GO enrichment and KEGG pathway analyses were performed using the DAVID (available online: http://david.ncifcrf.gov). The GO results of crucial terms for cellular component (CC), biological process (BP), and molecular function (MF) were obtained by importing the DEGs into DAVID, and p < 0.01 was considered statistically significant.
PPI network construction and analysis
PPI network comprising DEGs and hub genes were constructed using Metascape online analyses (https://metascape.org/gp/index.html#/main/step1), which could predict the interactions of proteins, and a combined score > 0.4 was considered statistically significant. Molecular interaction networks were constructed using Cytoscape (version 3.1.2; https://cytoscape.org/release_notes_3_2_1.html). The three most significant modules were identified by Molecular Complex Detection (MCOD, plug-in in Cytoscape software), which had MCODE scores > 3, false degree cut-off = 2, node score cut-off = 0.2, maximum depth = 100, and false k-score = 2. Functional enrichment analysis of each module was conducted using Metascape.
Identification, analysis, and validation of hub genes
The top ten genes with the highest degrees of connectivity were selected as hub genes. The hub genes within the four ESCC databases were visualized using cluster heatmaps drawn using GraphPad Prism heatmap (version 6.0; http://uone-tech.cn/graphpad-prism.html). The biological pathway and co-expression analyses of the ten hub genes was conducted using STRING (https://string-db.org/). We validated the expression levels and connections of the ten hub genes in ESCC tissues and matched normal tissues using GEPIA (http://gepia.cancer-pku.cn/index.html), an online database for analyzing gene expression profiles of tumors. The genetic alterations of the ten selected hub genes in ESCC were determined using cancer genomics prolifers obtained from the cBioCancer Genomic Portal (http://www.cbioportal.org/), which contains a large number of cancer genomics datasets.
Analysis of drug–hub gene interactions
The 10 hub genes served as the promising targets for searching for candidate drugs through the DGIdb (http://dgidb.genome.wustl.edu/), drugs from more than one database, or PubMed references that are approved by the FDA. The target network of the hub genes was constructed using STITCH (http://stitch.embl.de/).
Cell culture
Het-1A, TE-1, TE-10, KYSE30, KYSE150, KYSE450, and KYSE510 cells lines were purchased from the Type Culture Collection of the Chinese Academy of Science (Shanghai, China), and were cultured in a RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Biological Industries, Israel), 1% penicillin, and 1% streptomycin (Biological Industries, Israel) at 37 °C and 5% CO2.
RNA extraction and quantitative PCR
RNA was extracted from the cultured cells using the miRNeasy Mini Kit (QIAGEN, Germany), quantified using a spectrophotometer (BioTek, USA) and synthesized into complementary DNA using the GoScript Reverse Transcription Kit (Promega, USA), according to the manufacturer’s instructions. Quantitative PCR was performed using a 7900HT qPCR System (Applied Biosystems, USA) and GoTaq qPCR System Kit (Promega, USA), according to the manufacturer’s instructions. The primer sequences used were as follows: CDC6: sense primer: 5′-GCACAGGCTACAATCAGT-3′, anti-sense: 5′-CGAGGAGAACAGGTTACG-3′; GAPDH: sense primer: 5′-TCTCTGCTCCTCCTGTTC-3′, anti-sense: 5′-GTTGACTCCGACCTTCAC-3′. The relative mRNA expression of CDC6 was calculated using the 2−ΔΔCT method, normalized to that of endogenous GAPDH.
Western blot analysis
Cells were lysed using a RIPA lysis buffer (Beyotime, China), supplemented with 1 mM phenylmethylsulfonyl fluoride (Beyotime, China) and 1 mM phosphatase inhibitor (MCE, USA). Protein concentrations were quantified using an Enhanced BCA Protein Assay Kit (Beyotime,China), according to the manufacturer’s instructions. Protein samples were added to SDS loading buffer and boiled at 95 °C for 10 min. The denatured protein samples were electrophoresed on a 10% SDS-PAGE gel (Biosharp, China) and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Germany) using the wet transfer method (we cut the SDS-PAGE gels according to the position of target protein before the protein samples were transferred onto PVDF membranes). CDC6 is 63 kDa, while GAPDH is 36 kDa. The final PVDF membranes contained all these components. The membrane was blocked with 5% skim milk (Solarbio, China) at room temperature for 2 h. Next, the membranes were incubated with anti-CDC6 antibody (Abcam, USA) and anti-GAPDH antibody (Abcam, USA) at 4 °C overnight with gentle shaking. The membranes were washed with tris-buffered saline and Tween 20 (TBST) solution and then incubated with a horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Thermo Fisher Scientific, USA) at room temperature for 2 h. Protein bands were visualized using enhanced chemiluminescence (ECL) detection reagent (Beyotime, China). GAPDH was used as the internal control. All experiments were performed in triplicate. Densitometry analysis for western blots was conducted using ImageJ software (version 1.8.0, https://downloads.digitaltrends.com/imagej/windows).
Immunohistochemistry (IHC)
The Ultra Sensitive SP (Mouse/Rabbit) IHC Kit (Maixin, China), primary antibodies against CDC6 (Abcam, USA), and horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Abcam, USA) were used for IHC analysis. ADAB (3, 3′-Diaminobenzidine) kit (Maixin, China), which produces brown reactions, was used, according to the manufacturer’s instructions, for staining. The slides were counterstained with hematoxylin, dehydrated, made transparent, and mounted with neutral balsam. The IHC scores were independently assessed by two experienced pathologists who were blinded to any other information. Scores were based on the average staining intensity and staining ratio in five random high-power fields of view. Staining intensity scores were 0 (no staining), 1 (weak staining), 2 (moderate staining), and 3 (strong staining). We defined the staining ratio of positive cells as follows: 0 (< 5%), 1 (5–25%), 2 (26–50%), 3 (51–75%), and 4 (> 75%). The final IHC score ranged from 0 to 12 and was calculated as the staining intensity multiplied by the staining ratio. A final score ≥ 4 indicated high CDC6 expression, and < 4 indicated low CDC6 expression. Informed consent was obtained from all patients.
Statistical analysis
Data were analyzed using Statistical Package for the Social Sciences (SPSS, version 20) (https://spss.en.softonic.com/). Diagrams and ROC curve analyses were generated using GraphPad Prism software (version 6.0, San Diego, CA, USA). Dunnett's multiple comparison test was used to compare the CDC6 mRNA levels between ESCC and normal esophageal epithelial cell lines. All values are represented as mean ± standard deviation. The relationship between CDC6 and clinical factors was analyzed using χ2 independence or Fisher’s exact tests. Statistical significance was set at p < 0.05.
Informed consent
Tumor samples and clinical data were collected and used with the guidance of the Declaration of Helsinki and approved by the Ethics committee of the First Hospital of China Medical University.
Written informed consent has been obtained from the patients to publish this paper.
Data availability
The data used to support the findings of this study are available from the corresponding author upon request.
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Acknowledgements
We thank the NHC Key Laboratory of Immunodermatology (China Medical University) for experiments carried out. We also thank Professor Ruiqun Qi for experimental instructions.
Funding
This study was supported in part by Natural Science Foundation of Liaoning Province, China (NO. 2021-MS-191).
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S.G.Z., P.Y.Z. and H.Q.W. designed this research. H.Q.W., P.Y.Z. and P.S. performed the study and analyzed the results. H.Q.W. and P.S. wrote the manuscript. S.G.Z. and P.Y.Z. edited the manuscript and gave guidence. All authors read and approved the final manuscript.
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Wu, H., Zhu, P., Shu, P. et al. Screening and verification of hub genes in esophageal squamous cell carcinoma by integrated analysis. Sci Rep 14, 6894 (2024). https://doi.org/10.1038/s41598-024-57320-7
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DOI: https://doi.org/10.1038/s41598-024-57320-7
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