Abstract
Cerebral amyloid angiopathy (CAA), characterized by the deposition of amyloid aggregates in the walls of cerebral vasculature, is a major factor in intracerebral hemorrhage and vascular cognitive impairment and is also associated closely with Alzheimer’s disease (AD). We previously reported 99mTc-hydroxamamide (99mTc-Ham) complexes with a bivalent amyloid ligand showing high binding affinity for β-amyloid peptide (Aβ(1–42)) aggregates present frequently in the form in AD. In this article, we applied them to CAA-specific imaging probes and evaluated their utility for CAA-specific imaging. In vitro inhibition assay using Aβ(1–40) aggregates deposited mainly in CAA and a brain uptake study were performed for 99mTc-Ham complexes and all 99mTc-Ham complexes with an amyloid ligand showed binding affinity for Aβ(1–40) aggregates and very low brain uptake. In vitro autoradiography of human CAA brain sections and ex vivo autoradiography of Tg2576 mice were carried out for bivalent 99mTc-Ham complexes ([99mTc]SB2A and [99mTc]BT2B) and they displayed excellent labeling of Aβ depositions in human CAA brain sections and high affinity and selectivity to CAA in transgenic mice. These results may offer new possibilities for the development of clinically useful CAA-specific imaging probes based on the 99mTc-Ham complex.
Similar content being viewed by others
Introduction
Cerebral amyloid angiopathy (CAA) is a sporadic or familial disorder characterized by the deposition of amyloid aggregates, mainly β-amyloid peptide (Aβ), in the walls of arteries and less often capillaries of the central nervous system and belongs to the amyloidosis group1,2. CAA is present in 10–40% of the elderly3. In particular, at least a mild degree of CAA can be detected in up to 80% of patients with Alzheimer’s disease (AD)3, while severe CAA is present in approximately 25% of AD brains4.
CAA is a major cause of intracerebral hemorrhage (ICH) and vascular cognitive impairment5,6,7 and is also associated with small vessel diseases such as white matter hyperintensity and cerebral microbleeds6,8,9. CAA-associated ICH (CAA-ICH) comprises 5–20% of all spontaneous ICH in the elderly1,3. CAA-ICH is frequently a fatal condition and often recurs because of the multiple and widespread depositions of aggregated amyloid peptides in CAA brains1,7. Moreover, it was demonstrated that vascular diseases in the brain led to a decline of cognitive performance in the earliest stages of AD10,11.
Aβ(1–40) with a length of 40 amino acids is more soluble than longer Aβ(1–42) and is the main form in amyloid deposited in walls of blood vessels in CAA brains, while Aβ(1–42) is present more frequently in the form of senile plaque (SP) within the brain parenchyma in AD brains1,2. In patients with amyloidoses including CAA and AD, amyloid aggregates probably appear prior to onset of disease symptoms12,13; therefore, their detection in vivo may lead to an early diagnosis of the corresponding amyloidoses. Additionally, monitoring these amyloid aggregates in vivo may provide important information on the development of new medical techniques.
Brain biopsy is the gold standard for the diagnosis of CAA7; however, it is a highly invasive method. Although computed tomography (CT) and magnetic resonance imaging (MRI) are noninvasive and useful modalities for the diagnosis of CAA-ICH14,15, they detect intracerebral bleeding but not the deposition of amyloid aggregates; therefore, these indirect diagnostic techniques are unlikely to facilitate disease-specific diagnoses limited by the use of ICH as a surrogate marker for CAA. Accordingly, the development of a noninvasive technique to diagnose CAA-associated diseases specifically by the detection of amyloid using a probe is strongly needed.
Positron emission tomography (PET) and single photon emission computed tomography (SPECT) have generally been utilized as major in vivo imaging techniques to carry out the noninvasive diagnosis of amyloidoses. PET/SPECT can provide the information on localization of amyloid aggregates, while CT and MRI render the anatomical information. To date, many attempts to image Aβ aggregates constituting SP in AD brains using PET and SPECT tracers have been made. Several clinical studies using [11C]PIB, a neutral thioflavin-T analogue, have proved this utility for AD diagnosis16,17,18,19. More recently, [18F]florbetapir (Amyvid)17,20,21, [18F]flutemetamol (Vizamyl)16,22,23 and [18F]florbetaben (Neuraceq)24,25 have been approved by the US Food and Drug Administration for clinical AD diagnosis.
Similarly to SP in AD brains, there are several reports regarding the detection of cerebrovascular amyloid depositions using [11C]PIB26,27,28. However, since [11C]PIB is designed to penetrate the blood-brain barrier (BBB), it is considered to bind to not only vascular amyloid aggregates but also parenchymal amyloid aggregates, indicating that it detects amyloid depositions in the whole brain; therefore, [11C]PIB cannot help detecting SP as background signal in case of the diagnosis of CAA. Several efforts toward the development of imaging probes targeting Aβ deposition in CAA have been made. These probes, designed as fluorescent dye29, MRI contrast30,31,32, or PET/SPECT imaging31,32,33,34 agents, showed a potential use for CAA; however, in vivo specificity for Aβ aggregates in CAA was not demonstrated. Further research into the development of imaging probes for selective binding to Aβ deposited in the walls of the cerebral vasculature and to differentiate CAA from AD is desired.
To detect CAA but not SP, low brain uptake of an imaging probe targeting Aβ aggregates may be favorable33,34. We previously reported a series of 99mTc-hydroxamamide (99mTc-Ham) complexes with a multivalent amyloid ligand35 and utilized stilbene (SB) and benzothiazole (BT) as ligands for amyloid aggregates. These compounds are believed to hardly cross the BBB in vivo and their high binding affinity for Aβ aggregates is feasible for imaging CAA. However, in that report, the binding affinity of 99mTc-Ham complexes was evaluated using Aβ(1–42) aggregates present mainly in SP and less often CAA. It is generally accepted that compounds with high binding affinity for Aβ(1–42) aggregates except for antibodies can bind to other amyloid aggregates such as tau and α-synuclein36,37,38. Therefore, 99mTc-Ham complexes are considered to bind to Aβ(1–40) aggregates, the predominant amyloid found in CAA, similarly to Aβ(1–42) aggregates.
In the present study, we evaluated the binding affinity for Aβ(1–40) aggregates deposited mainly in CAA of 99mTc-Ham complexes with a monovalent or bivalent amyloid ligand ([99mTc]SB1, [99mTc]SB2, [99mTc]BT1 and [99mTc]BT2) (Fig. 1) and their utility for the in vivo specific detection of vascular amyloid aggregates but not parenchymal amyloid aggregates.
Results
Synthesis and 99mTc labeling
The 99mTc labeling reaction was performed by the complexation reaction using the Ham precursor, 99mTc-pertechnetate and tin (II) tartrate hydrate as a reducing agent35. The 99mTc complexation reaction with Ham derivatives provided two isomers of 99mTc-Ham complexes, as described in previous reports35,39. We defined the specific isomers with shorter retention times on reversed-phase high-performance liquid chromatography (RP-HPLC) as A-form ([99mTc]SB1A, [99mTc]SB2A, [99mTc]BT1A and [99mTc]BT2A) and the others as B-form ([99mTc]SB1B, [99mTc]SB2B, [99mTc]BT1B and [99mTc]BT2B).
99mTc-Ham complexes showed high binding affinity for Aβ(1–40) aggregates in solution
To evaluate binding affinity for Aβ(1–40) aggregates of 99mTc-Ham complexes, we performed an inhibition binding assay with PIB as a competitive ligand. A fixed concentration of Aβ(1–40) aggregates and the 99mTc-Ham complex were incubated with increasing concentrations of nonradioactive PIB. PIB showed IC50 values of 0.38, 0.45, 4.59, 3.37, 0.24, 0.99, 1.58 and 4.96 μM in the presence of [99mTc]SB1A, [99mTc]SB1B, [99mTc]SB2A, [99mTc]SB2B, [99mTc]BT1A, [99mTc]BT1B, [99mTc]BT2A and [99mTc]BT2B, respectively (Table 1).
Assessment of BBB permeability
To evaluate brain uptake of 99mTc-Ham complexes, biodistribution experiments of 99mTc-Ham complexes were performed in normal mice. We selected 18F-florbetapir as a control and compared the results of 99mTc-Ham complexes with that of 18F-florbetapir (Fig. 2). The brain uptake of [99mTc]SB1A, [99mTc]SB2A, [99mTc]BT1B and [99mTc]BT2B at 2 min postinjection was 0.37, 0.28, 0.36 and 0.37% injected dose (ID)/g, respectively. The radioactivity in the brains remained low until 60 min postinjection. The results of the biodistribution study are shown in Table S1 in Supplementary information.
99mTc-Ham complexes including bivalent amyloid ligand displayed excellent labeling of Aβ depositions in human CAA brain sections
The binding of [99mTc]SB2A and [99mTc]BT2B to Aβ depositions in brain sections from a CAA patient was evaluated by in vitro autoradiography. In CAA brain sections, [99mTc]SB2A intensively labeled Aβ depositions (Fig. 3A), while almost no accumulation of radioactivity was observed in the control brain sections (Fig. 3D). Furthermore, the labeling pattern was consistent with the immunohistochemical staining pattern observed in the same brain sections with anti-Aβ(1–40) antibody (Fig. 3B). In addition, the labeling of Aβ depositions with [99mTc]SB2A was blocked to a large extent with an excess of nonradioactive PIB (Fig. 3C). In vitro autoradiography with [99mTc]BT2B showed a similar result to that of [99mTc]SB2A (Fig. S1 in Supplementary information). Moreover, [99mTc]SB2A and [99mTc]BT2B also intensively labeled Aβ depositions in brain sections from another patient with CAA (Fig. S2 in Supplementary information).
99mTc-Ham complexes including bivalent amyloid ligand exhibited high affinity and selectivity to CAA in transgenic mice
To confirm the affinity of [99mTc]SB2A and [99mTc]BT2B for Aβ aggregates in a mouse brain, ex vivo autoradiography was performed using Tg2576 and wild-type mice (Fig. 4). The brains were removed at 30 min postinjection for autoradiography. Ex vivo autoradiograms with [99mTc]SB2A displayed intensive labeling of Aβ depositions in the transgenic mice (Fig. 4A,B) but not the age-matched controls (Fig. 4C). The labeling pattern on autoradiograms was partially consistent with the staining pattern observed in the same brain sections from Tg2576 mice with thioflavin-S, a dye commonly used to stain Aβ depositions (Fig. 4D,E), while there was no marked staining in the wild-type mouse brain sections (Fig. 4F). However, some Aβ depositions were not labeled with [99mTc]SB2A in Tg2576 mouse brain sections. To confirm whether [99mTc]SB2A labeled Aβ aggregates deposited within vascular or parenchyma, the same sections were immunostained with anti-CD31 antibody, a marker for endothelial cells (Fig. 4G–I)40,41. The accumulation of radioactivity on autoradiograms was observed only at Aβ depositions labeled with both thioflavin-S and anti-CD31 antibody (Fig. 4B,E and H, red arrows), while no radioactive spots were observed at Aβ depositions labeled with thioflavin-S, not anti-CD31 antibody (Fig. 4E, white arrowheads). Furthermore, ex vivo autoradiography with [99mTc]BT2B showed a similar result to that of [99mTc]SB2A (Fig. S3 in Supplementary information).
Discussion
We previously reported 99mTc-Ham complexes with a bivalent amyloid ligand showing high binding affinity for Aβ(1–42) aggregates35. In the present study, we evaluated their utility as CAA-specific imaging probes. Recently, several new 99mTc-labeled CAA imaging agents were reported42,43,44. In spite of the fact that they were synthesized under heating and acidic conditions, 99mTc-Ham complexes can be prepared under mild conditions (non-heating and neutral), indicating that 99mTc-Ham complexes may be superior to the other CAA-imaging probes reported previously. In vitro binding study using Aβ(1–40) aggregates, a major form of amyloid in CAA, exhibited that the amyloid ligand dimers ([99mTc]SB2 and [99mTc]BT2) bound to Aβ(1–40) aggregates more strongly than their monomers ([99mTc]SB1 and [99mTc]BT1) and A-form of 99mTc-Ham complexes with BT derivatives showed lower binding affinity than B-form, as demonstrated in our previous report using Aβ(1–42) aggregates (Table 1). However, specific isomers of 99mTc-Ham complexes with SB derivatives displayed similar binding affinity for Aβ(1–40) aggregates to those of the other isomers, while significant differences between IC50 values with two isomers of SB derivatives were shown in the inhibition assay using Aβ(1–42) aggregates35. All 99mTc-Ham complexes showed blocked binding to amyloid aggregates with a very high concentration (μM order) of PIB, although the Aβ imaging probes reported previously have a binding affinity equal to or lower than that of PIB, suggesting that they have a much higher binding affinity than any other tracers targeting Aβ including CAA-specific imaging probes44. Among eight 99mTc-Ham complexes, both [99mTc]SB2A and [99mTc]BT2B showed high binding affinity for Aβ(1–40) aggregates (4.59 and 4.96 μM, respectively), which was higher than those of any other 99mTc-Ham complexes.
According to the result of the inhibition assay using Aβ(1–40) and Aβ(1–42) aggregates, brain uptake studies were performed for only specific isomers with the higher binding affinity for Aβ aggregates (A-form of SB derivatives and B-form of BT derivatives). [99mTc]SB1A, [99mTc]SB2A, [99mTc]BT1B and [99mTc]BT2B displayed much lower initial brain uptake than [18F]florbetapir under similar experimental conditions (4.90%ID/g at 2 min postinjection) (Fig. 2)45, while [18F]florbetapir has proved its utility for imaging Aβ plaques in the brain. In addition, 99mTc-Ham complexes also showed a lower initial brain entry than even other CAA imaging probes reported previously (0.61–1.21%ID/g at that time)44. These results suggest that 99mTc-Ham complexes could hardly cross the BBB. Therefore, they may be incapable of binding to Aβ aggregates deposited within the brain parenchyma. According to the results of binding affinity for Aβ(1–40) and Aβ(1–42) aggregates in vitro and brain uptake in normal mice ex vivo, further studies were conducted using [99mTc]SB2A and [99mTc]BT2B with high binding affinity for Aβ aggregates and very low brain uptake.
In vitro autoradiography of human CAA brain sections with [99mTc]SB2A showed intensive labeling of Aβ depositions (Fig. 3A), confirmed by immunostaining of the same brain sections with anti-Aβ(1–40) antibody (Fig. 3B). Many 99mTc-labeled Aβ imaging probes with preferable binding affinity have exhibited no marked labeling of Aβ depositions in human brain sections; however, Jia et al. recently reported a 99mTc-labeled tracer showing positive autoradiography results for brain sections from AD patients44. As well as those results, 99mTc-Ham complexes showed excellent labeling of Aβ depositions in human brain sections. Additionally, a blocking study with nonradioactive PIB confirmed the specific binding of [99mTc]SB2A to Aβ depositions in CAA brain sections (Fig. 3C). In vitro autoradiography with [99mTc]BT2B showed specific binding to Aβ depositions in CAA brain sections as well as [99mTc]SB2A (Fig. S1 in Supplementary information). In addition, two bivalent 99mTc-Ham complexes, [99mTc]SB2A and [99mTc]BT2B, also showed intensive labeling of Aβ depositions in brain sections from another patient with CAA (Fig. S2 in Supplementary information).
Ex vivo autoradiography with [99mTc]SB2A displayed specific binding to Aβ aggregates in the living Tg2576 mouse brain (Fig. 4A,B) but not wild-type mouse brain (Fig. 4C). Since Tg2576 mice are known to overproduce Aβ aggregates in the brain, they have been commonly used to evaluate the specific binding of Aβ aggregates in experiments in vitro and in vivo45,46. The accumulation of radioactivity in Tg2576 mouse brain sections was observed only at the presence of both amyloid aggregates (Fig. 4D,E) and endothelial cells (Fig. 4G,H), suggesting that [99mTc]SB2A selectively bound to amyloid aggregates deposited along vessels but not within parenchyma. In addition, [99mTc]BT2B displayed specific detection of CAA in Tg2576 mice (Fig. S3 in Supplementary information). These results are consistent with the biodistribution study showing the low brain entry of 99mTc-Ham complexes. These findings in the present study suggest that our bivalent 99mTc-Ham complexes, [99mTc]SB2A and [99mTc]BT2B, can specifically detect CAA in vivo. However, these tracers seemed to label areas in the cortex that are not apparent in the thioflavin-S staining. Thioflavin-S has much lower affinity (Kd: μM order) than useful Aβ imaging probes reported previously such as PIB, florbetapir (Kd: nM order)19,47. An in vitro inhibition assay showed that our 99mTc-labeled compounds blocked binding to Aβ aggregates due to a much higher concentration of unlabeled-PIB, suggesting that our compounds have a much higher binding affinity than other Aβ and CAA imaging agents reported previously. Accordingly, it is considered that thioflavin-S can detect fewer depositions of amyloid than our compounds. In addition, we also carried out ex vivo autoradiography using perfused mouse brains and obtained results showing differences with Tg2576 and wild-type mice (data not shown), suggesting that radioactivity was derived from tracers binding to depositions of amyloid and not from tracers contained in the blood. Although the possibility that the BBB is leaky in the brains of AD patients has been suggested in several reports48,49, it has remained controversial whether or not the BBB dysfunction depends on the stage of AD. Not all studies have indentified an index of BBB disruption in AD brains50,51,52, but Zipser et al. recently reported that dysfunction of the BBB could increase stepwisely with the degree of pathology in AD53, indicating that the BBB should be intact in an early stage of preclinical AD. Moreover, CAA imaging probes should be used in the preclinical stage of the disease when the BBB functions normally. Therefore, 99mTc-Ham complexes developed in the present study, which may be incapable of penetrating the BBB, can serve as CAA-specific imaging probes for the early diagnosis of AD.
In addition, we performed an in vivo SPECT/CT study with [99mTc]SB2A using Tg2576 and wild-type mice at 30 min postinjection (Fig. S4 in Supplementary information). Although ex vivo autoradiography demonstrated the specificity of [99mTc]SB2A for CAA, [99mTc]SB2A was not differentially distributed in the brains of Tg2576 and wild-type mice in vivo. The radioactivity accumulation was observed mostly in the limbic region of the brain, which seemed to be derived from the blood. This inference is supported by the observations that blood vessels were rich in this region of the brain confirmed by immunostaining of CD31 (Fig. 4G,I) and a biodistribution study which suggested that [99mTc]SB2A has a high retention rate in the blood (5.13%ID/g at 30 min postinjection, Table S1 in Supplementary information). Although the influence of radioactivity in the cerebral blood could be removed by perfusion in ex vivo autoradiography examination, it was inevitable to detect radioactivity in the blood as a background signal in the in vivo SPECT study. Furthermore, we carried out an in vivo SPECT imaging study at a later time point (120 min postinjection) of [99mTc]SB2A. However, we obtained a similar result to that of at 30 min postinjection, suggesting that the radioactivity in the blood still remained at 120 min postinjection (Fig. S5 in Supplementary information). Therefore, further acceleration of the clearance of 99mTc-labeled probes from the blood pool is essential for the development of in vivo imaging probes targeting CAA. The introduction of a hydrophilic substituted group including hydroxyl and carboxyl groups may constitute one of the strategies to enhance the clearance of probes from the blood. For instance, the replacement of the dimethylamino group in [99mTc]SB2A with a hydroxyl group reduces its lipophilicity, contributing to lower binding to plasma proteins. This modification of probes should facilitate the more rapid clearance of [99mTc]SB2A from the blood, leading to a lower background signal that can bring about an increase in the specific signal of the probes on binding to CAA.
In the current study, we applied bivalent 99mTc-Ham complexes that we reported previously to imaging probes targeting CAA. All 99mTc-Ham complexes including a monovalent or bivalent amyloid ligand showed binding affinity for Aβ(1–40) aggregates in vitro and very low brain uptake in normal mice ex vivo. In vitro autoradiography showed specific binding of 99mTc-Ham complexes including a bivalent amyloid ligand ([99mTc]SB2A and [99mTc]BT2B) with high binding affinity in the inhibition assay to Aβ depositions in brain sections from a CAA patient. Additionally, [99mTc]SB2A and [99mTc]BT2B displayed excellent and selective labeling of Aβ depositions in vessels but not parenchyma in mouse brains. The results suggest that [99mTc]SB2A and [99mTc]BT2B have potential as CAA-specific imaging probes. Although the in vivo SPECT/CT study with [99mTc]SB2A showed no marked difference in radioactivity accumulation in the brain between Tg2576 and wild-type mice, the findings in the present study reveal new possibilities of developing clinically useful CAA imaging probes based on the 99mTc-Ham complex. Further optimization to improve the clearance of 99mTc-Ham complexes from the blood is underway.
Methods
General
All reagents were obtained commercially and used without further purification unless otherwise indicated. PIB was purchased from ABX (Saxony, Germany). Na99mTcO4 was purchased from Nihon Medi-Physics Co., Ltd. (Tokyo, Japan) or was obtained from a commercial 99Mo/99mTc generator (Ultra-Techne Kow; FUJIFILM RI Pharma Co., Ltd., Tokyo, Japan). RP-HPLC was performed with a Shimadzu system (SHIMADZU, Kyoto, Japan, a LC-20AT pump with a SPD-20A UV detector, λ = 254 nm) with a Cosmosil C18 column (Nacalai Tesque, Kyoto, Japan, 5C18-AR-II, 4.6 mm × 150 mm) using a mobile phase (10 mM phosphate buffer (pH 7.4)/acetonitrile: 0 min 3/2 to 30 min 3/7) delivered at a flow rate of 1.0 mL/min.
Animals
Animal experiments were conducted in accordance with our institutional guidelines and were approved by the Kyoto University Animal Care Committee. Male ddY mice were purchased from Japan SLC, Inc. (Shizuoka, Japan). Female Tg2576 mice and wild-type mice were purchased from Taconic Farms, Inc. (New York, USA). Animals were fed standard chow and had free access to water. All efforts were made to minimize suffering.
Human brain tissues
Experiments involving human subjects were performed in accordance with relevant guidelines and regulations and were approved by the ethics committee of Kyoto University and National Cerebral and Cardiovascular Center. Informed consent was secured from all subjects in this study. Postmortem brain tissues from autopsy-confirmed cases of CAA (female, 67 years old and female, 85 years old) and a control (male, 73 years old) were obtained from the Graduate School of Medicine, Kyoto University, National Cerebral and Cardiovascular Center and BioChain Institute, Inc. (California, USA), respectively.
Synthesis and 99mTc labeling
99mTc-Ham complexes ([99mTc]SB1, [99mTc]SB2, [99mTc]BT1 and [99mTc]BT2) were prepared as we reported previously35. In brief, to solutions of 0.2 mg Ham precursors ((Z)-4-((E)-4-(dimethylamino)styryl)-N’-hydroxybenzimidamide, (Z)-2-(4-(dimethylamino)phenyl)-N’-hydroxybenzo[d]thiazole-6-carboximidamide and (Z)-4-(dimethylamino)-N’-hydroxybenzimidamide) in acetate/ethanol (1/4, 200 μL) were added 100 μL Na99mTcO4 solution and 15 μL tin (II) tartrate hydrate solution [2 mg tin (II) tartrate hydrate (7.50 μmol) dissolved in water (2.5 mL)]. The reaction mixtures were incubated at room temperature for 30 min and purified by RP-HPLC. The 99mTc-Ham complexes were analyzed by analytical RP-HPLC on a Cosmosil C18 column (5C18-AR-II, 4.6 mm × 150 mm) with a solvent of phosphate buffer (10 mM, pH 7.4)/acetonitrile (0 min 3/2 to 30 min 3/7) as the mobile phase at a flow rate of 1.0 mL/min. The radioactivity of the 99mTc-labeled compounds was recorded for 30 min.
Competitive inhibition assay using Aβ(1–40) aggregates in solution
A solid form of Aβ(1–40) was purchased from the Peptide Institute (Osaka, Japan). Aggregation was carried out by gently dissolving the peptide (0.50 mg/mL) in phosphate-buffered saline (PBS) (pH 7.4). The solution was incubated at 37 °C for 42 h with gentle and constant shaking. A mixture containing 50 μL Aβ(1–40) aggregates (final conc., 1.25 μg/mL), 50 μL 99mTc-Ham complex (final conc., 8.3 kBq/mL), 50 μL PIB (final conc., 64 pM–125 μM in 30% EtOH) and 850 μL of 30% EtOH was incubated at room temperature for 3 h. The mixture was filtered through Whatman GF/B filters (Whatman, Kent, U.K.) using a Brandel M-24 cell harvester (Brandel, Maryland, USA) and the radioactivity of the filters containing the bound 99mTc-Ham complex was measured using a γ counter (Wallac 1470 Wizard; PerkinElmer, Massachusetts, USA). Values for the half-maximal inhibitory concentration (IC50) were determined from displacement curves using GraphPad Prism 5.0 (GraphPad Software, Inc., California, USA).
Ex vivo biodistribution in normal mice
A saline solution (100 μL) of 99mTc-Ham complexes (20 kBq) containing EtOH (10 μL) was injected directly into the tail vein of ddY mice (male, 5 weeks old). The mice were sacrificed at 2, 10, 30 and 60 min postinjection. The organs of interest were removed and weighed and radioactivity was measured using a γ counter (PerkinElmer). The %ID/g of samples was calculated by comparing the sample counts with the count of the diluted initial dose.
In vitro autoradiography of human CAA brain sections
Six micrometer thick serial human brain sections of paraffin-embedded blocks were used for autoradiography. To completely deparaffinize the sections, they were incubated in xylene for 30 min two times and in 100% EtOH for 1 min two times. Subsequently, they were subjected to 1-min incubation in 90% EtOH and 1-min incubation in 70% EtOH, followed by a 5-min wash in water. Each slide was incubated with a 50% EtOH solution of [99mTc]SB2A or [99mTc]BT2B (370 kBq/mL) at room temperature for 1 h. For blocking experiments, the adjacent sections were incubated with a 50% EtOH solution of [99mTc]SB2A or [99mTc]BT2B (370 kBq/mL) in the presence of nonradioactive PIB (1.0 mM). The sections were washed in 50% EtOH for 3 min two times and exposed to a BAS imaging plate (Fuji Film, Tokyo, Japan) for 2 h. Autoradiographic images were obtained using a BAS5000 scanner system (Fuji Film). After autoradiographic examination, the same sections were immunostained by an antibody against Aβ(1–40) to confirm the presence of Aβ depositions. For immunohistochemical staining of Aβ(1–40), the sections were autoclaved for 15 min in 0.01 M citric acid buffer (pH 6.0) to activate the antigen. After three 5-min incubations in PBS-Tween 20 (PBST), they were incubated with anti-Aβ(1–40) primary antibody (BA27; Wako, Osaka, Japan) at room temperature overnight. Subsequently, they were incubated in PBST for 5 min three times and incubated with biotinylated goat anti-mouse IgG (Wako) at room temperature for 3 h. After three 5-min incubations in PBST, the sections were incubated with Streptavidin-Peroxidase complex at room temperature for 30 min. After three 5-min incubations in PBST, they were incubated with diaminobenzidine (Merck, Hesse, Germany) as a chromogen for 5 min. After washing with water, the sections were observed under a microscope (BIOREVO BZ-9000; Keyence Corp., Osaka, Japan).
Ex vivo autoradiography using Tg2576 and wild-type mice
Tg2576 transgenic mice (female, 29 months old) and wild-type mice (female, 29 months old) were used as the AD model and age-matched control, respectively. A saline solution (150 μL) of [99mTc]SB2A or [99mTc]BT2B (18.5 MBq) containing EtOH (30 μL) was injected through the tail vein. The mice were sacrificed at 30 min postinjection. The brains were immediately removed, embedded in carboxymethylcellulose solution and then frozen in a dry ice/hexane bath. Sections of 30 μm were cut and exposed to a BAS imaging plate (Fuji Film) overnight. Autoradiographic images were obtained using a BAS5000 scanner system (Fuji Film). After autoradiographic examination, the same sections were stained by thioflavin-S to confirm the presence of Aβ depositions. For thioflavin-S fluorescent staining, the sections were immersed in a 100 μM thioflavin-S solution containing 50% EtOH for 3 min, washed in 50% EtOH for 1 min two times and examined using a microscope (Keyence Corp.) equipped with a GFP-BP filter set. Additionally, the same sections were immunostained by anti-CD31 antibody to confirm the presence of endothelial cells. For immunohistochemical staining of CD31, the sections were incubated in PBST for 5 min three times and incubated with anti-CD31 primary antibody (SZ31; Abcam, Cambridgeshire, U.K., dilution 1:50) at room temperature overnight. After three 5-min incubations in PBST, anti-rabbit secondary antibody (Dako, California, USA) incubation was carried out at room temperature for 3 h. Subsequently, the sections were incubated in PBST for 5 min three times and incubated with diaminobenzidine (Merck) as a chromogen for 5 min. After washing with water, the sections were observed under a microscope (Keyence Corp.).
Additional Information
How to cite this article: Iikuni, S. et al. Imaging of Cerebral Amyloid Angiopathy with Bivalent 99mTc-Hydroxamamide Complexes. Sci. Rep. 6, 25990; doi: 10.1038/srep25990 (2016).
References
Biffi, A. & Greenberg, S. M. Cerebral amyloid angiopathy: a systematic review. J. Clin. Neurol. 7, 1–9 (2011).
Gahr, M., Nowak, D. A., Connemann, B. J. & Schonfeldt-Lecuona, C. Cerebral amyloidal angiopathy−a disease with implications for neurology and psychiatry. Brain Res. 1519, 19–30 (2013).
Jellinger, K. A. Alzheimer disease and cerebrovascular pathology: an update. J. Neural Transm. 109, 813–836 (2002).
Ellis, R. J. et al. Cerebral amyloid angiopathy in the brains of patients with Alzheimer’s disease: the CERAD experience, Part XV. Neurology. 46, 1592–1596 (1996).
Greenberg, S. M., Gurol, M. E., Rosand, J. & Smith, E. E. Amyloid angiopathy-related vascular cognitive impairment. Stroke. 35, 2616–2619 (2004).
Viswanathan, A. & Greenberg, S. M. Cerebral amyloid angiopathy in the elderly. Ann. Neurol. 70, 871–880 (2011).
Mehndiratta, P. et al. Cerebral amyloid angiopathy-associated intracerebral hemorrhage: pathology and management. Neurosurg. Focus. 32, E7 (2012).
Smith, E. E. Leukoaraiosis and stroke. Stroke. 41, S139–143 (2010).
Holland, C. M. et al. Spatial distribution of white-matter hyperintensities in Alzheimer disease, cerebral amyloid angiopathy and healthy aging. Stroke. 39, 1127–1133 (2008).
Snowdon, D. A. et al. Brain infarction and the clinical expression of Alzheimer disease. The nun study. JAMA. 277, 813–817 (1997).
Esiri, M. M., Nagy, Z., Smith, M. Z., Barnetson, L. & Smith, A. D. Cerebrovascular disease and threshold for dementia in the early stages of Alzheimer’s disease. Lancet. 354, 919–920 (1999).
Braak, H. & Braak, E. Frequency of stages of Alzheimer-related lesions in different age categories. Neurobiol. Aging. 18, 351–357 (1997).
Thal, D. R., Rub, U., Orantes, M. & Braak, H. Phases of Aβ-deposition in the human brain and its relevance for the development of AD. Neurology. 58, 1791–1800 (2002).
Walker, D. A., Broderick, D. F., Kotsenas, A. L. & Rubino, F. A. Routine use of gradient-echo MRI to screen for cerebral amyloid angiopathy in elderly patients. AJR Am. J. Roentgenol. 182, 1547–1550 (2004).
Zhan, R. Y. et al. Study of clinical features of amyloid angiopathy hemorrhage and hypertensive intracerebral hemorrhage. J. Zhejiang Univ. Sci. 5, 1262–1269 (2004).
Hatashita, S. et al. [18F]Flutemetamol amyloid-beta PET imaging compared with [11C]PIB across the spectrum of Alzheimer’s disease. Eur. J. Nucl. Med. Mol. Imaging. 41, 290–300 (2014).
Ni, R., Gillberg, P. G., Bergfors, A., Marutle, A. & Nordberg, A. Amyloid tracers detect multiple binding sites in Alzheimer’s disease brain tissue. Brain. 136, 2217–2227 (2013).
Klunk, W. E. et al. Imaging brain amyloid in Alzheimer’s disease with Pittsburgh Compound-B. Ann. Neurol. 55, 306–319 (2004).
Mathis, C. A. et al. Synthesis and evaluation of 11C-labeled 6-substituted 2-arylbenzothiazoles as amyloid imaging agents. J. Med. Chem. 46, 2740–2754 (2003).
Wong, D. F. et al. In vivo imaging of amyloid deposition in Alzheimer disease using the radioligand [18F]AV-45 (florbetapir F 18). J. Nucl. Med. 51, 913–920 (2010).
Lin, K. J. et al. Whole-body biodistribution and brain PET imaging with [18F]AV-45, a novel amyloid imaging agent−a pilot study. Nucl. Med. Biol. 37, 497–508 (2010).
Lundqvist, R. et al. Implementation and validation of an adaptive template registration method for 18F-flutemetamol imaging data. J. Nucl. Med. 54, 1472–1478 (2013).
Nelissen, N. et al. Phase 1 study of the Pittsburgh compound B derivative 18F-flutemetamol in healthy volunteers and patients with probable Alzheimer disease. J. Nucl. Med. 50, 1251–1259 (2009).
Zhang, W. et al. F-18 Polyethyleneglycol stilbenes as PET imaging agents targeting Aβ aggregates in the brain. Nucl. Med. Biol. 32, 799–809 (2005).
Becker, G. A. et al. PET quantification of 18F-florbetaben binding to β-amyloid deposits in human brains. J. Nucl. Med. 54, 723–731 (2013).
Ly, J. V. et al. 11C-PIB binding is increased in patients with cerebral amyloid angiopathy-related hemorrhage. Neurology. 74, 487–493 (2010).
Johnson, K. A. et al. Imaging of amyloid burden and distribution in cerebral amyloid angiopathy. Ann. Neurol. 62, 229–234 (2007).
Dierksen, G. A. et al. Spatial relation between microbleeds and amyloid deposits in amyloid angiopathy. Ann. Neurol. 68, 545–548 (2010).
Han, B. H. et al. Resorufin analogs preferentially bind cerebrovascular amyloid: potential use as imaging ligands for cerebral amyloid angiopathy. Mol. Neurodegener. 6, 86 (2011).
Poduslo, J. F. et al. Targeting vascular amyloid in arterioles of Alzheimer disease transgenic mice with amyloid β protein antibody-coated nanoparticles. J. Neuropathol. Exp. Neurol. 70, 653–661 (2011).
Agyare, E. K. et al. Engineering theranostic nanovehicles capable of targeting cerebrovascular amyloid deposits. J. Control. Release. 185, 121–129 (2014).
Jaruszewski, K. M. et al. Multimodal nanoprobes to target cerebrovascular amyloid in Alzheimer’s disease brain. Biomaterials. 35, 1967–1976 (2014).
Nabuurs, R. J. et al. In vivo detection of amyloid-β deposits using heavy chain antibody fragments in a transgenic mouse model for Alzheimer’s disease. PLoS One. 7, e38284 (2012).
Zha, Z. et al. Multidentate 18F-polypegylated styrylpyridines as imaging agents for Aβ plaques in cerebral amyloid angiopathy (CAA). J. Med. Chem. 54, 8085–8098 (2011).
Iikuni, S. et al. Enhancement of binding affinity for amyloid aggregates by multivalent interactions of 99mTc-hydroxamamide complexes. Mol. Pharm. 11, 1132–1139 (2014).
Bagchi, D. P. et al. Binding of the radioligand SIL23 to α-synuclein fibrils in Parkinson disease brain tissue establishes feasibility and screening approaches for developing a Parkinson disease imaging agent. PLoS One. 8, e55031 (2013).
Antoni, G. et al. In vivo visualization of amyloid deposits in the heart with 11C-PIB and PET. J. Nucl. Med. 54, 213–220 (2013).
Matsumura, K. et al. Synthesis and biological evaluation of novel styryl benzimidazole derivatives as probes for imaging of neurofibrillary tangles in Alzheimer’s disease. Bioorg. Med. Chem. 21, 3356–3362 (2013).
Nakayama, M. et al. Hydroxamamide as a chelating moiety for the preparation of 99mTc-radiopharmaceuticals III. Characterization of various 99mTc-hydroxamamides. Appl. Radiat. Isot. 48, 571–577 (1997).
Cui, X. B., Guo, X. & Chen, S. Y. Response gene to complement 32 deficiency causes impaired placental angiogenesis in mice. Cardiovasc. Res. 99, 632–639 (2013).
Merlini, M., Meyer, E. P., Ulmann-Schuler, A. & Nitsch, R. M. Vascular β-amyloid and early astrocyte alterations impair cerebrovascular function and cerebral metabolism in transgenic arcAβ mice. Acta. Neuropathol. 122, 293–311 (2011).
Jia, J., Cui, M., Dai, J. & Liu, B. 2-Phenylbenzothiazole conjugated with cyclopentadienyl tricarbonyl [CpM(CO)3] (M = Re, 99mTc) complexes as potential imaging probes for β-amyloid plaques. Dalton Trans. 44, 6406–6415 (2015).
Wang, X., Cui, M., Jia, J. & Liu, B. 99mTc-labeled-2-arylbenzoxazole derivatives as potential Aβ imaging probes for single-photon emission computed tomography. Eur. J. Med. Chem. 89, 331–339 (2015).
Jia, J., Cui, M., Dai, J. & Liu, B. 99mTc(CO)3-labeled benzothiazole derivatives preferentially bind cerebrovascular amyloid: potential use as imaging agents for cerebral amyloid angiopathy. Mol. Pharm. 12, 2937–2946 (2015).
Yoshimura, M. et al. Structure-activity relationships and in vivo evaluation of quinoxaline derivatives for PET imaging of β-amyloid plaques. ACS Med. Chem. Lett. 4, 31–35 (2013).
Alpar, A. et al. Different dendrite and dendritic spine alterations in basal and apical arbors in mutant human amyloid precursor protein transgenic mice. Brain Res. 1099, 189–198 (2006).
Choi, S. R. et al. Preclinical properties of 18F-AV-45: a PET agent for Aβ plaques in the brain. J. Nucl. Med. 50, 1887–1894 (2009).
Skoog, I. et al. A population study on blood-brain barrier function in 85-year-olds: relation to Alzheimer’s disease and vascular dementia. Neurology. 50, 966–971 (1998).
De Reuck, J. L. The significance of small cerebral bleeds in neurodegenerative dementia syndromes. Aging Dis. 3, 307–312 (2013).
Mecocci, P. et al. Blood-brain-barrier in a geriatric population: barrier function in degenerative and vascular dementias. Acta Neurol. Scand. 84, 210–213 (1991).
Kay, A. D. et al. CSF and serum concentrations of albumin and IgG in Alzheimer's disease. Neurobiol. Aging. 8, 21–25 (1987).
Rozemuller, J. M., Eikelenboom, P., Kamphorst, W. & Stam, F. C. Lack of evidence for dysfunction of the blood-brain barrier in Alzheimer’s disease: an immunohistochemical study. Neurobiol. Aging. 9, 383–391 (1988).
Zipser, B. D. et al. Microvascular injury and blood-brain barrier leakage in Alzheimer’s disease. Neurobiol. Aging. 28, 977–986 (2007).
Acknowledgements
This research was supported by a grant from the Japan Society for the Promotion of Science (JSPS) through the “Funding Program for Next Generation World-Leading Researchers (NEXT Program),” initiated by the Council for Science and Technology Policy (CSTP) and JSPS KAKENHI Grant Number 26293274. We thank Department of Neurology, Kyoto University for providing brain samples of a CAA case.
Author information
Authors and Affiliations
Contributions
S.I., M.O., H.W., H.K. and H.S. designed the study. S.I., M.O., H.W., K.M., M.Y., Y.O., H.I.-U. and M.I. carried out the experiments. S.I., M.O., H.W., H.K. and H.S. analyzed the data. S.I. and M.O. wrote the paper. All authors discussed the results and reviewed the manuscript.
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Electronic supplementary material
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Iikuni, S., Ono, M., Watanabe, H. et al. Imaging of Cerebral Amyloid Angiopathy with Bivalent 99mTc-Hydroxamamide Complexes. Sci Rep 6, 25990 (2016). https://doi.org/10.1038/srep25990
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep25990
- Springer Nature Limited
This article is cited by
-
Development of a hydroxamamide-based bifunctional chelating agent to prepare technetium-99m-labeled bivalent ligand probes
Scientific Reports (2021)
-
Flexible multidentate benzyldiamine derivatives with high affinity for β-amyloid in cerebral amyloid angiopathy
Molecular Diversity (2021)
-
Rhenium and technetium complexes of thioamide derivatives of pyridylhydrazine that bind to amyloid-β plaques
JBIC Journal of Biological Inorganic Chemistry (2018)
-
Can brain impermeable BACE1 inhibitors serve as anti-CAA medicine?
BMC Neurology (2017)