Abstract
Background
Periodontitis triggers tooth loss and affects the health of population worldwide. Emerging evidence hints that circular RNAs (circRNAs) are involved in various diseases, including periodontitis. This study aimed to investigate the role of circ_0099630 in the progression of periodontitis.
Methods
Periodontitis cell model was constructed by treating human periodontal ligament cells (HPDLCs) with lipopolysaccharide (LPS). Quantitative real-time PCR was used to analyze the expression of circ_0099630, microRNA-409-3p (miR-409-3p) and toll-like receptor 4 (TLR4) mRNA. Western blot was used for detecting protein levels of TLR4, cleaved-caspase 3, Bcl-2, CyclinD1 and NF-κB signaling markers. For function analyses, cell proliferation was assessed by CCK-8 assay and EdU assay. The releases of pro-inflammation factors were monitored by ELISA kits. The potential relationship between miR-409-3p and circ_0099630 or TLR4 was verified by dual-luciferase reporter assay, RIP assay and pull-down assay.
Results
The expression of circ_0099630 and TLR4 was elevated in periodontitis patients and LPS-treated HPDLCs. LPS induced HPDLC proliferation inhibition, apoptosis and inflammatory responses, while circ_0099630 knockdown or TLR4 knockdown alleviated these injuries. Besides, TLR4 overexpression reversed the inhibitory effect of circ_0099630 knockdown on LPS-induced HPDLC injuries. Mechanism analysis showed that circ_0099630 positively regulated TLR4 expression by acting as miR-409-3p sponge. MiR-409-3p restoration largely ameliorated LPS-induced HPDLC injuries by depleting TLR4. Moreover, LPS activated the NF-κB signaling pathway, while circ_0099630 knockdown inhibited the activity of NF-κB signaling via the miR-409-3p/TLR4 axis.
Conclusion
Circ_0099630 knockdown relieved LPS-induced HPDLC injury by miR-409-3p/TLR4 axis, suggesting that circ_0099630 might be a potential target for periodontitis treatment.
Similar content being viewed by others
Introduction
Periodontitis is a chronic infectious disease of periodontal tissue, mainly affected by microorganisms in dental plaque [1]. The main characteristics of periodontitis are gum inflammation, periodontal pocket formation and alveolar bone resorption, leading to tooth loosening or loss [2, 3]. Nowadays, periodontitis is a widespread oral disease, and the incidence rate is increasing year by year worldwide [4, 5]. Increasing evidence shows that periodontitis is closely related to systemic diseases, including diabetes, hypertension, and cardiovascular diseases [6,7,8]. Therefore, it is important to clarify the potential pathogenesis of periodontitis to improve its treatment effects.
Periodontal ligament (PDL) tissue has the ability to stimulate periodontal regeneration, and periodontal ligament cells (PDLCs) are indispensable in the regeneration of periodontal tissue [9]. Numerous studies show that non-coding RNAs participate in the proliferation and differentiation of PDLCs in vitro, thus involving in the pathogenesis of periodontitis [10, 11]. Circular RNAs (circRNAs) are a class of non-coding RNAs, and its deregulation is closely associated with the progression of human diseases [12]. RNA-sequencing technology provided a growing number of circRNAs that were differently expressed in gingival tissues from periodontitis patients and healthy individuals [13]. According to the data, we found that circ_0099630 was highly upregulated in periodontitis patients [13]. Circ_0099630 was rarely investigated in any disorders in previous studies, and the functions of circ_0099630 were largely unknown. It was of great significance to explore the role of circ_0099630 in periodontitis to further understand the pathogenesis of periodontitis.
Toll-like receptor 4 (TLR4) is widely known as an important regulator of inflammatory responses and participates in various human inflammatory diseases [14, 15]. TLR4 was reported to be upregulated in chronic periodontitis [16, 17], and the downregulation of TLR4 contributed to the inhibition of inflammation in periodontitis [18]. Nonetheless, the mechanism of TLR4 in this disorder was not fully understood.
Accumulating studies indicate that circRNAs serve as microRNA (miRNA) sponges to be implicated in posttranscriptional regulation [19], which provides a new action mode of circRNAs. By targeting miRNAs, circRNAs can relieve the inhibition of miRNA on downstream target genes. Though bioinformatics analysis, both circ_0099630 and TLR4 3’ untranslated region (3’-UTR) harbored response elements with miR-409-3p. Therefore, we speculated that circ_0099630 might regulate TLR4 via sponging miR-409-3p and thus regulate multiple biological processes. However, the interactions between circ_0099630 and TLR4 were not addressed in previous studies.
In this study, we constructed inflammatory cell models of periodontitis by treating human periodontal ligament cells (HPDLCs) with lipopolysaccharide (LPS). We investigated the function of circ_0099630 and TLR4 on proliferation, apoptosis and inflammation of LPS-treated HPDLCs. Besides, we screened miR-409-3p and proposed the circ_0099630/miR-409-3p/TLR4 network to illustrate the functional mechanism of circ_0099630 in LPS-mediated HPDLC injuries, aiming to provide additional opinion for periodontitis treatment.
Materials and methods
Periodontal tissue collection
A total of 20 periodontitis patients and 20 normal controls (teeth for orthodontic) recruited at First Hospital Affiliated to Lanzhou University. After the root surface was washed with PBS, the middle 1/3 of the periodontal ligament tissues in the root was scraped and stored at -80 °C. Patients with other oral, systemic diseases and antibiotic intake within 1 month were excluded from this study. The clinicopathologic characteristics of periodontitis patients and normal controls are listed in Table 1. Written informed consent was obtained from each subject. This project was approved by the Ethics Committee of First Hospital Affiliated to Lanzhou University [Approval number: 202,111,045].
Cell model construction
Healthy periodontal ligament tissues were collected from subjects underwent orthodontic treatment. HPDLCs were isolated from periodontal ligament tissues at the one third of the root of molars through the enzyme digestion. HPDLCs were cultured in DMEM (GIBCO, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO) in a 37℃ incubator containing 5% CO2. HPDLCs at 3 to 6 passages were used in this study.
Inflammatory cell models of periodontitis were established by treating HPDLCs with P. gingivalis LPS (1 µg/mL; Sigma-Aldrich, St. Louis, MO, USA). After time intervals of 24 h, HPDLCs were collected for the following experiments.
Quantitative real-time PCR (qPCR)
RNA samples were isolated using TRIzol reagent (Takara, Dalian, China). For cDNA synthesis, the PrimeScript™ 1st Strand cDNA Synthesis Kit (Takara) or Mir-X miRNA First-Strand Synthesis Kit (Clontech, Mountain View, CA, USA) was used according to the protocols. Subsequently, qPCR was performed using the TB Green Fast qPCR Mix (Takara) or Mir-X miRNA TB Green Kit (Clontech) according to the protocols. We used the 2−ΔΔCt method to calculate relative expression with GAPDH or U6 as an internal reference. The sequences of primers were:
circ_0099630, F: 5’-TCGGCCAAAGGAAGAATGAC-3’ and R: 5’-TTGATGAAAATGACCCATGACG-3’; miR-409-3p, F: 5’-GAATGTTGCTCGGTGAACCCCT-3’ and R: 5’-GAACATGTCTGCGTATCTC-3’; TLR4, F: 5’-AGACCTGTCCCTGAACCCTAT-3’ and R: 5’-CGATGGACTTCTAAACCAGCCA-3’; GAPDH, F: 5’-TCGGAGTCAACGGATTTGGT-3’ and R: 5’-TTCCCGTTCTCAGCCTTGAC-3’; U6, F: 5’-GCTTCGGCAGCACATATACTAAAAT-3’ and R: 5’-CGCTTCACGAATTTGCGTGTCAT-3’;
Western blot
The procedures of western blot were accordance with the previous description [20]. Briefly, protein was extracted by RUPA buffer and quantified by BCA Kit (Beyotime, Shanghai, China). Then, 30 µg protein was separated by SDS-PAGE gel and transferred onto PVDF membranes. Membrane was blocked by non-fat milk and incubated with primary antibodies. After washing with TBST for 3 times, membrane was treated with secondary antibody. Protein signals were detected using ECL reagent (Beyotime). The primary antibodies were purchased from Abcam (Cambridge, MA, USA), including anti-TLR4 (ab13556), anti-cleaved-caspase 3 (ab2302), anti-Bcl-2 (ab692), anti-CyclinD1 (ab16663), anti-p-TAK1 (ab109404), anti-p-IκBα (ab133462), anti-p65 (ab16502) and anti-GAPDH (ab9485). The secondary antibodies were also purchased from Abcam, including goat anti-rabbit IgG (ab205718) and goat anti-mouse IgG (ab205719).
Cell transfection
Circ_0099630-specific small interference RNA (si-circ_0099630), TLR4 overexpression fusion vector (TLR4) and their negative control (si-NC and vector) were synthesized by Genepharma (Shanghai, China). MiR-409-3p-specific mimic (miR-409-3p), inhibitor (anti-miR-409-3p) and matched negative controls (miR-NC and anti-NC) were all obtained from Ribobio (Guangzhou, China). These oligonucleotides or vectors were transfected into cells using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA).
CCK-8 assay
After transfection, LPS-treated HPDLCs were seeded into 96-well plates and then cultured for 24 h, 48 or 72 h. At different time points, cells in each well were treated with 10 µL CCK-8 reagent (Sigma-Aldrich) for another 2 h. Cell viability was then detected using an iMARK microplate reader (Bio-Rad, Hercules, CA, USA) at 450 nm.
EdU assay
To assess cell proliferation, EdU assay was performed using the Cell-Light EdU Apollo567 in Vitro Kit (Ribobio). After transfection, cells were treated with EdU reagent for 2 h and then fixed in PBS containing 4% paraformaldehyde for 15 min. Cells were next stained with dyeing solution for 30 min and counterstained with DAPI buffer. The positive-staining cells were observed and counted under a fluorescence microscope (Olympus, Tokyo, Japan).
Flow cytometry assay
For cell apoptosis analysis, cells were detected by the Annexin V-FITC Apoptosis Detection Kit (Beyotime) in line with the protocol. In brief, a total of 5 × 104 cells were suspended into 195 µL Annexin V-FITC binding buffer, followed by treatment with 5 µL Annexin V-FITC and 10 µL propidium iodide (PI). The apoptotic cells were detected by a flow cytometer (Beckman Coulter, Miami, FL, USA).
For cell cycle analysis, cells were detected using the Cell Cycle Analysis Kit (Beyotime) according to the protocol. In brief, cells were collected and washed with PBS. Then, cells were fixed in 70% ethanol overnight and stained with PI staining buffer (containing RNase A). The content of DNA was checked using a flow cytometer (Beckman Coulter).
ELISA
To monitor the releases of pro-inflammatory factors, including TNF-α, IL-1β and IL-6, ELISA was carried out using TNF-α Human ELISA Kit (Invitrogen), IL-1β Human ELISA Kit (Invitrogen) and IL-6 Human ELISA Kit (Invitrogen). All procedures were accordance with the manufacturer’s instructions.
Bioinformatics tools
The potential miRNAs interacted with circ_0099630 were predicted by circinteractome (https://circinteractome.nia.nih.gov/). The potential miRNAs interacted with TLR4-3’UR were predicted by starbase (http://starbase.sysu.edu.cn/). Circinteractome prediction methods are as follows: Open the website: https://circinteractome.nia.nih.gov/?tdsourcetag=s_pcqq_aiomsg, select “miRNA Target Sites”, input “circRNA ID: hsa_circ_0099630”, click “miRNA Target Search”. Starbase database prediction method are as below: Open the website: https://rnasysu.com/encori/index.php, select “microRNAs-Target”, click “microRNAs-mRNA”, select “Genome: the human”, select “Target Gene: TLR4”. Then, the miRNAs predicted by circinteractome and starbase database were compared, and Venn diagram analysis was performed to analyze the miRNAs that could directly combine with circ_0099630 and TLR4, respectively.
Dual-luciferase reporter assay
The mutant sequences of circ_0099630 and TLR4-3’UTR were designed, and the wild-type (WT) and mutant-type (MUT) reporter plasmids of circ_0099630 and TLR4-3’UTR were constructed by yanzaibio Co., Ltd. (Shanghai, China), shortly naming as circ_0099630-WT, circ_0099630-MUT, TLR4-3’UTR-WT and TLR4-3’UTR-MUT. These reporter plasmids were separately transfected with miR-409-3p or miR-NC into HPDLCs. After time interval of 48 h, luciferase activity was checked using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).
RIP assay
RIP assay was performed using the Imprint RNA Immunoprecipitation Kit (Sigma-Aldrich). HPDLCs were lysed, and cell lysates were incubated with Protein A magnetic beads conjugated with human Ago2 antibody (anti-Ago2; Sigma-Aldrich) or control mouse IgG antibody (anti-IgG; Sigma-Aldrich). RNA samples bound to beads were eluted and isolated for qPCR analysis.
Pull-down assay
Biotin-labeled miR-409-3p probe (Bio-miR-409-3p) and negative control probe (Bio-NC) were provided by Ribobio. Pull-down assay was implemented using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol. RNA compounds pulled down by Bio-miR-409-3p were eluted and isolated for qPCR analysis.
Statistical analysis
All assays were repeated for three times to collect statistical data. The data were processed using GraphPad Prism 7 (GraphPad Software Inc., San Diego, CA, USA) and shown as the mean ± standard deviation (SD). Statistical difference in groups was compared using Student’s t-test (2 groups) and ANOVA followed by Tukey’s post-hoc test (multiple groups). Statistical significance was defined when P-value less than 0.05.
Results
Circ_0099630 and TLR4 were overexpressed in periodontal tissues and LPS-treated HPDLCs
The expression of circ_0099630 was detected in tissues and cell models at first. As shown in Fig. 1A, the expression of circ_0099630 was significantly enhanced in periodontal tissues from periodontitis patients compared to NC. Likewise, the expression of TLR4 mRNA was also significantly increased in periodontitis patients (Fig. 1B). Besides, we found that TLR4 mRNA expression was positively correlated with circ_0099630 expression in periodontal tissues of periodontitis (Fig. 1C). The expression of circ_0099630 was notably increased in LPS-treated HPDLCs (Fig. 1D), and the high TLR4 expression was observed in LPS-treated HPDLCs at both mRNA and protein levels (Fig. 1E F). The aberrant expression of circ_0099630 and TLR4 hinted that they were involved in periodontitis development.
LPS-induced cell injuries in HPDLCs were alleviated by circ_0099630 knockdown
The expression of circ_0099630 was effectively reduced in HPDLCs by si-circ_0099630 to study the function of circ_0099630 (Fig. 2A). The capacity of cell proliferation was determined by CCK-8 assay and EdU assay. The data showed that HPDLC proliferation was largely suppressed by LPS, while circ_0099630 knockdown partly recovered this effect (Fig. 2B C). Flow cytometry assay showed that LPS-induced HPDLC apoptosis was substantially attenuated by circ_0099630 knockdown (Fig. 2D). Also, LPS significantly induced cell cycle arrest at the G0/G1 phase, while this arrest was largely alleviated by circ_0099630 knockdown (Fig. 2E). The protein levels of cleaved-caspase 3 and CyclinD1 were elevated, while the protein level of Bcl-2 was declined in LPS-induced HPDLCs. However, circ_0099630 knockdown reduced the levels of cleaved-caspase 3 and CyclinD1 but enhanced the level of Bcl-2 (Fig. 2F). Moreover, the releases of IL-6, IL-8 and TNF-α were stimulated by LPS, while circ_0099630 knockdown inhibited the releases of these pro-inflammatory factors (Fig. 2G and I). We summarized from these data that circ_0099630 knockdown alleviated LPS-induced injures of HPDLCs.
LPS-induced cell injuries in HPDLCs were alleviated by TLR4 knockdown
The endogenous level of TLR4 protein was also effectively decreased in HPDLCs to study the function of TLR4 (Fig. 3A). LPS-suppressed proliferative capacity of HPDLCs was largely restored by TLR4 knockdown (Fig. 3B C). In contrast, LPS-induced apoptosis rate of HPDLCs was limited by TLR4 knockdown (Fig. 3D). As for cell cycle, we discovered that TLR4 knockdown partly relieved LPS-induced cell cycle arrest (Fig. 3E). The protein levels of cleaved-caspase 3 and CyclinD1 enhanced by LPS administration were substantially reduced by TLR4 downregulation, while the protein level of Bcl-2 inhibited by LPS administration was substantially enhanced by TLR4 knockdown (Fig. 3F). The releases of IL-6, IL-8 and TNF-α were stimulated by LPS but largely suppressed by TLR4 knockdown (Fig. 3G and I). The data hinted that TLR4 knockdown alleviated LPS-induced cell injuries of HPDLCs.
TLR4 overexpression reversed the effects of circ_0099630 knockdown in LPS-treated HPDLCs
The protein level of TLR4 was strikingly decreased in LPS-treated HPDLCs transfected with si-circ_0099630, while the protein level of TLR4 was largely recovered in LPS-treated HPDLCs transfected with si-circ_0099630 + TLR4 (Fig. 4A). In function, cell proliferative capacity was largely recovered in LPS-treated HPDLCs transfected with si-circ_0099630 but repressed in LPS-treated HPDLCs transfected with si-circ_0099630 + TLR4 (Fig. 4B C). The apoptosis rate of HPDLCs was alleviated by alone circ_0099630 knockdown, while additional TLR4 overexpression promoted the apoptosis rate of HPDLCs (Fig. 4D). TLR4 overexpression substantially induced cell cycle arrest that was alleviated by circ_0099630 knockdown alone (Fig. 4E). The expression of cleaved-caspase 3 and CyclinD1 was reduced in LPS-treated HPDLCs transfected with si-circ_0099630 but recovered in cells transfected with si-circ_0099630 + TLR4, while the expression of Bcl-2 was enhanced by si-circ_0099630 but repressed by circ_0099630 + TLR4 in LPS-treated HPDLCs (Fig. 4F). The releases of IL-6, IL-8 and TNF-α in LPS-treated HPDLCs were significantly blocked by si-circ_0099630 transfection but promoted by si-circ_0099630 + TLR4 transfection (Fig. 4G and I). These findings indicated that circ_0099630 knockdown alleviated LPS-induced injures of HPDLCs by downregulating TLR4.
Circ_0099630 functioned as Mir-409-3p sponge to regulate TLR4 expression
To explore the interaction between circ_0099630 and TLR4, we screened miRNAs that harbored response elements with both circ_0099630 and TLR4. The analysis from circinteractome and starbase showed that only 2 miRNAs (miR-409-3p and miR-498) were found to harbor binding sites with both circ_0099630 and TLR4 (Fig. 5A). In HPDLCs with circ_0099630 knockdown, the expression of miR-409-3p was notably increased, while miR-198 expression was rarely changed (Fig. 5B). We chose miR-409-3p for the following assays. The expression of miR-409-3p was strikingly enhanced in cells transfected with miR-409-3p mimic (Fig. 5C). For dual-luciferase reporter assay, the WT and MUT reporter plasmids of circ_0099630 and TLR4-3’UTR were constructed, and the sequences were shown in Fig. 5D and E. Dual-luciferase reporter assay presented that luciferase activity was significantly declined in HPDLCs transfected with miR-409-3p and circ_0099630-WT plasmid or TLR4-3’UTR-WT plasmid (Fig. 5F and G). RIP assay presented that circ_0099630, miR-409-3p and TLR4 were abundantly detected in the anti-Ago2-enriched compounds compared to anti-IgG (Fig. 5H). Pull-down assay presented that circ_0099630 and TLR4 could be abundantly enriched by Bio-miR-409-3p probe compared to Bio-NC (Fig. 5I). The expression of miR-409-3p was strikingly reduced in cells transfected with anti-miR-409-3p (Fig. 5J). The expression of TLR4 mRNA and protein was notably suppressed in HPDLCs transfected with miR-409-3p compared to miR-NC, while the expression of TLR4 was notably elevated in HPDLCs transfected with anti-miR-409-3p compared to anti-NC (Fig. 5K L). In addition, the expression of miR-409-3p was shown to be decreased in LPS-treated HPDLCs (Fig. 5M). These data suggested that circ_0099630 functioned as miR-409-3p sponge to regulate TLR4 expression.
MiR-409-3p restoration alleviated LPS-induced cell injuries of HPDLCs
We next investigated the function of miR-409-3p in LPS-treated HPDLCs. LPS-impaired cell proliferative capacity was largely recovered by miR-409-3p restoration (Fig. 6A and B). LPS-induced cell apoptosis of HPDLCs was partially suppressed by miR-409-3p restoration (Fig. 6C and D). Besides, LPS-induced cell cycle arrest was also notably alleviated by miR-409-3p restoration (Fig. 6E). The protein levels of cleaved-caspase 3 and CyclinD1 were inhibited by miR-409-3p, while the protein level of Bcl-2 was enhanced by miR-409-3p in LPS-treated HPDLCs (Fig. 6F). Moreover, the releases of IL-6, IL-8 and TNF-α stimulated by LPS were largely suppressed in LPS-treated HPDLCs transfected with miR-409-3p (Fig. 6G and I). The data suggested that LPS-induced cell injuries of HPDLCs were also alleviated by miR-409-3p restoration.
TLR4 overexpression reversed the effects of mir-409-3p restoration in LPS-treated HPDLCs
The expression of TLR4 protein was significantly declined in LPS-treated HPDLCs transfected with miR-409-3p, while the expression of TLR4 was largely recovered in LPS-treated HPDLCs transfected with miR-409-3p + TLR4 (Fig. 7A). In terms of function, cell proliferative capacity was notably recovered by miR-409-3p restoration but largely repressed by TLR4 overexpression in LPS-treated HPDLCs (Fig. 7B C). Cell apoptosis was largely blocked in LPS-treated HPDLCs transfected with miR-409-3p but partially enhanced in cells transfected with miR-409-3p + TLR4 (Fig. 7D). Cell cycle arrest in LPS-treated HPDLCs was largely alleviated by miR-409-3p transfection, while cell cycle was substantially arrested at the G0/G1 phase by miR-409-3p + TLR4 transfection (Fig. 7E). The expression of cleaved-caspase 3 and CyclinD1 was notably suppressed in LPS-treated HPDLCs transfected with miR-409-3p but recovered in cells transfected with miR-409-3p + TLR4, while the expression of Bcl-2 was opposite to them (Fig. 7F). The releases of IL-6, IL-8 and TNF-α were markedly blocked in LPS-treated HPDLCs transfected with miR-409-3p but accelerated in LPS-treated HPDLCs transfected with miR-409-3p + TLR4 (Fig. 7G and I). These data indicated that miR-409-3p restoration alleviated LPS-induced HPDLC injuries by degrading TLR4.
NF-κB signaling pathway activated in LPS-treated HPDLCs was modulated by the circ_0099630/miR-409-3p/TLR4 axis
NF-κB signaling pathway was documented to be activated in various human diseases. Interestingly, we found that TLR4 expression was inhibited by si-circ_0099630 in LPS-treated HPDLCs but recovered by si-circ_0099630 + anti-miR-409-3p (Fig. 8A and B). The protein levels of p-TAK1, p-IκBα and p65 were shown to be increased in LPS-induced HPDLCs, while si-circ_0099630 transfection weakened the protein levels of p-TAK1, p-IκBα and p65. In addition, the protein levels of p-TAK1, p-IκBα and p65 were partially recovered in LPS-induced HPDLCs with si-circ_0099630 + anti-miR-409-3p transfection (Fig. 8A C-8E). The data hinted that circ_0099630 knockdown alleviated LPS-induced HPDLC injuries by inhibiting the activity of the NF-κB signaling pathway by targeting the miR-409-3p/TLR4 axis.
Discussion
The present study explored the role and mechanism of circ_0099630 in periodontitis. We mainly discovered that the expression of circ_0099630 and TLR4 was elevated in periodontitis patients and LPS-treated HPDLCs. Knockdown of circ_0099630 or TLR4 partially restored LPS-induced HPDLCs proliferation inhibition, apoptosis and inflammation. In addition, we for the first time proposed the circ_0099630/miR-409-3p/TLR4 network to illustrate the new mechanism of circ_0099630 in periodontitis.
HPDLCs are responsible for tooth support, collagen production and tissue regeneration [21]. LPS is the major component of the cell wall of gram-negative bacteria. Periodontitis bacteria, especially LPS, is widely shown to induce HPDLCs to secrete pro-inflammatory factors, thus playing a key role in the progression of periodontitis [22, 23]. In our study, we constructed cell models of periodontitis by treating HPDLCs with LPS. At present, circ_0099630 was only reported to be upregulated in periodontitis tissues through the data of circRNA expression profiles [13]. To verify this result, we checked the expression of circ_0099630 in clinical periodontal tissues and found that circ_0099630 was also highly expressed in periodontitis patients. Besides, circ_0099630 knockdown alleviated LPS-induced proliferation inhibition, apoptosis and inflammatory responses of HPDLCs, suggesting that circ_0099630 might promote periodontitis progression.
Toll-like receptors (TLRs) are involved in the immune response by recognizing patterns presented by bacteria and other pathogens [24]. TLR4 has been shown to be closely implicated in inflammatory responses that caused gingivitis and periodontitis [25]. TLR4 was upregulated in LPS-treated HPDLCs, and tripartite motif-containing 52 (TRIM52) knockdown alleviated LPS-induced inflammatory responses by inhibiting TLR4 [26]. The expression of TLR4 was also elevated in the experimental periodontitis rat, and Baicalin played anti-inflammatory effects in periodontitis rat by depleting the expression of TLR4 [27]. Consistent with these studies, we showed that TLR4 was upregulated in periodontitis patients and LPS-treated HPDLCs. TLR4 knockdown attenuated LPS-induced HPDLC proliferative inhibition, apoptosis and inflammatory responses. Interestingly, we found that TLR4 overexpression could reversed the effects of circ_0099630 knockdown and recovered LPS-induced injuries in HPDLCs, confirming that TLR4 might be a potential target for periodontitis treatment.
To realize the interactions between circ_0099630 and TLR4, we screened potential miRNAs that harbored binding sites with both circ_0099630 and TLR4 3’-UTR. As a result, the target relationship between miR-409-3p and circ_0099630 or TLR4 was confirmed by dual-luciferase reporter assay, RIP assay and pull-down assay. MiR-409-3p has been reported to play crucial roles in various human diseases and cancers [28, 29]. However, the role of miR-409-3p in periodontitis is still unknown. Our study discovered that miR-409-3p restoration relieved LPS-induced proliferation inhibition, apoptosis and inflammation in HPDLCs, while TLR4 overexpression abolished these effects, suggesting that miR-409-3p alleviated LPS-induced injuries in HPDLCs by sequestering TLR4. Moreover, TLR4 expression was notably decreased in HPDLCs with circ_0099630 knockdown, while additional miR-409-3p deficiency recovered TLR4 expression. We summarized that circ_0099630 positively regulated TLR4 expression by serving as miR-409-3p sponge.
By reviewing the previous studies, we found that the NF-κB signaling pathway was one of the downstream pathways of TLR4 [30]. It was reported that TLR4 activated the NF-κB signaling pathway to induce the release of pro-inflammatory factors [31], and Resveratrol played protective effects against experimental periodontitis in mice via inhibiting the phosphorylation of NF-κB signaling molecules, including p65, p38MAPK, and STAT3 [32]. Similarly, TRIM52 knockdown inhibited the release of pro-inflammatory factors by suppressing TLR4 expression and the phosphorylation of downstream NF-κB signaling molecules, including TAK1, IKK-α/β, IκBα and p65 [26]. In our study, we found that the phosphorylation of TAK1, IκBα and p65 was activated in LPS-treated HPDLCs, while circ_0099630 knockdown weakened the phosphorylation of these molecules. The data hinted that circ_0099630 knockdown inhibited the activity of the NF-κB signaling pathway through the miR-409-3p/TLR4 axis, thus alleviating LPS-induced injuries in HPDLCs.
Collectively, circ_0099630 and TLR4 were highly expressed in periodontitis patients and LPS-treated HPDLCs. Circ_0099630 knockdown alleviated LPS-induced dysfunctions and injuries of HPDLCs through miR-409-3p-mediated TLR4 inhibition. Besides, circ_0099630 knockdown might regulate the inhibition of the NF-κB signaling pathway via the miR-409-3p/TLR4 axis, which needed further verification. Our study provided a new insights into the understanding of periodontitis development.
Data Availability
Data are however available from the authors upon reasonable request.
References
Bustamante M, Oomah BD, Mosi-Roa Y, Rubilar M, Burgos-Diaz C. Probiotics as an Adjunct Therapy for the treatment of Halitosis, Dental Caries and Periodontitis. Probiotics Antimicrob Proteins. 2020;12(2):325–34.
Kaur G, Grover V, Bhaskar N, Kaur RK. Jain A. Periodontal Infectogenomics. Inflamm Regen. 2018;38:8.
Divaris K, Monda KL, North KE, Olshan AF, Lange EM, Moss K, et al. Genome-wide association study of periodontal pathogen colonization. J Dent Res. 2012;91(7 Suppl):21S–8S.
Zhang Q, Li Z, Wang C, Shen T, Yang Y, Chotivichien S, et al. Prevalence and predictors for periodontitis among adults in China, 2010. Glob Health Action. 2014;7:24503.
Eke PI, Dye BA, Wei L, Slade GD, Thornton-Evans GO, Borgnakke WS, et al. Update on prevalence of periodontitis in adults in the United States: NHANES 2009 to 2012. J Periodontol. 2015;86(5):611–22.
Merchant AT, Virani SS. Evaluating Periodontal Treatment to Prevent Cardiovascular Disease: challenges and possible solutions. Curr Atheroscler Rep. 2017;19(1):4.
Wu CZ, Yuan YH, Liu HH, Li SS, Zhang BW, Chen W, et al. Epidemiologic relationship between periodontitis and type 2 Diabetes Mellitus. BMC Oral Health. 2020;20(1):204.
Munoz Aguilera E, Suvan J, Buti J, Czesnikiewicz-Guzik M, Barbosa Ribeiro A, Orlandi M, et al. Periodontitis is associated with Hypertension: a systematic review and meta-analysis. Cardiovasc Res. 2020;116(1):28–39.
Kuang Y, Hu B, Feng G, Xiang M, Deng Y, Tan M, et al. Metformin prevents against oxidative stress-induced senescence in human periodontal ligament cells. Biogerontology. 2020;21(1):13–27.
Zhong YX, Li WS, Liao LS, Liang L. LncRNA CCAT1 promotes cell proliferation and differentiation via negative modulation of miRNA-218 in human DPSCs. Eur Rev Med Pharmacol Sci. 2019;23(9):3575–83.
Wang F, Chen X, Han Y, Xi S, Wu G. circRNA CDR1as regulated the Proliferation of Human Periodontal Ligament Stem Cells under a Lipopolysaccharide-Induced Inflammatory Condition. Mediators Inflamm. 2019;2019:1625381.
Qu S, Zhong Y, Shang R, Zhang X, Song W, Kjems J, et al. The emerging landscape of circular RNA in life processes. RNA Biol. 2017;14(8):992–9.
Li J, Xie R. Circular RNA expression profile in gingival tissues identifies circ_0062491 and circ_0095812 as potential treatment targets. J Cell Biochem. 2019;120(9):14867–74.
Katare PB, Nizami HL, Paramesha B, Dinda AK, Banerjee SK. Activation of toll like receptor 4 (TLR4) promotes cardiomyocyte apoptosis through SIRT2 dependent p53 deacetylation. Sci Rep. 2020;10(1):19232.
Wu CP, Bi YJ, Liu DM, Wang LY. Hsa-miR-375 promotes the progression of inflammatory bowel Disease by upregulating TLR4. Eur Rev Med Pharmacol Sci. 2019;23(17):7543–9.
Rojo-Botello NR, Garcia-Hernandez AL, Moreno-Fierros L. Expression of toll-like receptors 2, 4 and 9 is increased in gingival tissue from patients with type 2 Diabetes and chronic periodontitis. J Periodontal Res. 2012;47(1):62–73.
Lappin DF, Sherrabeh S, Erridge C. Stimulants of toll-like receptors 2 and 4 are elevated in saliva of periodontitis patients compared with healthy subjects. J Clin Periodontol. 2011;38(4):318–25.
Li K, Lv G, Pan L. Sirt1 alleviates LPS induced inflammation of periodontal ligament fibroblasts via downregulation of TLR4. Int J Biol Macromol. 2018;119(249 – 54.
Kulcheski FR, Christoff AP, Margis R. Circular RNAs are miRNA sponges and can be used as a new class of biomarker. J Biotechnol. 2016;238:42–51.
Pan J, Zhao L, Liu J, Wang G. Inhibition of circular RNA circ_0138959 alleviates pyroptosis of human gingival fibroblasts via the microRNA-527/caspase-5 axis. Bioengineered. 2022;13(1):1908–20.
Ali M, Yang F, Jansen JA, Walboomers XF. Lipoxin suppresses inflammation via the TLR4/MyD88/NF-kappaB pathway in periodontal ligament cells. Oral Dis. 2020;26(2):429–38.
Fitzsimmons TR, Ge S, Bartold PM. Compromised inflammatory cytokine response to P. Gingivalis LPS by fibroblasts from inflamed human gingiva. Clin Oral Investig. 2018;22(2):919–27.
Aidoukovitch A, Anders E, Dahl S, Nebel D, Svensson D, Nilsson BO. The host defense peptide LL-37 is internalized by human periodontal ligament cells and prevents LPS-induced MCP-1 production. J Periodontal Res. 2019;54(6):662–70.
Chrzeszczyk D, Konopka T, Zietek M. Polymorphisms of toll-like receptor 4 as a risk factor for Periodontitis: Meta-Analysis. Adv Clin Exp Med. 2015;24(6):1059–70.
Ding YS, Zhao Y, Xiao YY, Zhao G. Toll-like receptor 4 gene polymorphism is associated with chronic periodontitis. Int J Clin Exp Med. 2015;8(4):6186–92.
Liu P, Cui L, Shen L. Knockdown of TRIM52 alleviates LPS-induced inflammatory injury in human periodontal ligament cells through the TLR4/NF-kappaB pathway. Biosci Rep. 2020;40(8).
Sun JY, Li DL, Dong Y, Zhu CH, Liu J, Li JD, et al. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis. Int Immunopharmacol. 2016;36:86–93.
Ventriglia G, Mancarella F, Sebastiani G, Cook DP, Mallone R, Mathieu C, et al. Mir-409-3p is reduced in plasma and islet immune infiltrates of NOD diabetic mice and is differentially expressed in people with type 1 Diabetes. Diabetologia. 2020;63(1):124–36.
Bai R, Weng C, Dong H, Li S, Chen G, Xu Z. MicroRNA-409-3p suppresses Colorectal cancer invasion and Metastasis partly by targeting GAB1 expression. Int J Cancer. 2015;137(10):2310–22.
Hussey SE, Liang H, Costford SR, Klip A, DeFronzo RA, Sanchez-Avila A, et al. TAK-242, a small-molecule inhibitor of toll-like receptor 4 signalling, unveils similarities and differences in lipopolysaccharide- and lipid-induced inflammation and insulin resistance in muscle cells. Biosci Rep. 2012;33(1):37–47.
Kuper C, Beck FX, Neuhofer W. Toll-like receptor 4 activates NF-kappaB and MAP kinase pathways to regulate expression of proinflammatory COX-2 in renal medullary collecting duct cells. Am J Physiol Renal Physiol. 2012;302(1):F38–46.
Zhen L, Fan DS, Zhang Y, Cao XM, Wang LM. Resveratrol ameliorates experimental periodontitis in diabetic mice through negative regulation of TLR4 signaling. Acta Pharmacol Sin. 2015;36(2):221–8.
Acknowledgements
Not applicable.
Funding
Not applicable.
Author information
Authors and Affiliations
Contributions
Bing Han designed and performed the research; Jin Che analyzed the data; Hongyan Qi wrote the manuscript. All authors read and approved the final manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Animal studies were performed in compliance with the ARRIVE guidelines and the Basel Declaration. All animals received humane care according to the National Institutes of Health (USA) guidelines. The research has been carried out in accordance with the World Medical Association Declaration of Helsinki, and that all subjects provided written informed consent. This project was approved by the Ethics Committee of First Hospital Affiliated to Lanzhou University [Approval number: 202111045].
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no conflicts of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Qi, H., Han, B. & Che, J. Circ_0099630 knockdown alleviates lipopolysaccharide-induced injuries of human periodontal ligament cells through the inhibition of TLR4 by releasing miR-409-3p. BMC Oral Health 23, 922 (2023). https://doi.org/10.1186/s12903-023-03622-7
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s12903-023-03622-7