Abstract
In recent years rapidly growing antibiotic resistance has increased interest toward natural products, especially essential oils because of their various effects. The aim of this study was to identify the chemical composition of the commercial Origanum onites essential oil (EO) and to investigate the antimicrobial activity by disc diffusion and dilution methods, against ten different ATCC strains, including eight bacteria, two yeasts and seventy-nine clinical nosocomial Escherichia coli isolates that produce extended spectrum beta lactamase (ESBL). The chemical composition of EO was analyzed by GC and GC-MS. The major compounds of the EO were determined as carvacrol (51.4%) followed by linalool (11.2%), p-cymene (8.9%) and γ-terpinene (6.7%). O. onites EO had antimicrobial activity against all standard strains and inhibited microbial growth of ESBL positive E. coli isolates. According to our results, O. onites EO may be an alternative to synthetic drug, used in combination with other antibiotics for treatment of infection caused by multidrug resistant bacteria after testing toxic effects and irritation at preferred doses on human.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Adams, R. P. (2001) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Co., Illinois, USA.
Akgül, A., Kıvanç, M. (1998) Inhibitory effects of selected Turkish spices and oregano components on some food borne fungi, Intern. J. Food. Microbiol. 6, 263–268.
Askun, T., Tumen, G., Satil, F., Ates, M. (2009) Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharm. Biol. 47, 563–571.
Baser, K. H. C. (2004) Domestic uses of Oregano in Turkey. In: Kitzios, S. E. (ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis, London, pp. 111.
Baser, K. H. C. (2008) Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Curr. Pharm. Design. 14, 106–3019.
Baydar, H., Sagdiç, O., Ozkan, G., Karadoğan, T. (2004) Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 15, 169–172.
Beklem Bostancıoğlu, R., Kürkçüoğlu, M., Başer, K. H., Koparal, A. T. (2012) Assessment of antiangiogenic and anti-tumoral potentials of Origanum onites L. essential oil. Food Chem. Toxicol. 50, 2002–2008.
Boyraz, N., Ozcan, M. (2006) Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savory (Satureja hortensis L.) growing wild in Turkey. Int. J. Food Microbiol. 107, 238–42
Cantón, R., Novais, A., Valverde, A., Machado, E., Peixe, L., Baquero, F. (2008) Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clin. Microbiol. Infect. 14, 144–153.
CLSI (2013) Performance Standards for Antimicrobial Susceptibility Testing, Twenty-Third Informational Supplement, Wayne PA, M100-S23, Vol. 33, No.1, January.
Cosentino, S., Tuberoso, C. I., Pisano, B., Satta, M., Mascia, V., Arzedi, E., Palmas, F. (1999) Palmas, In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett. Appl. Microbiol. 29, 130–135.
Demirci, F., Paper, D. H., Franz, G., Başer, K. H. C. (2004) Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane assay. J. Agr. Food Chem. 52, 251–254.
EUCAST (2016) Breakpoint tables for interpretation of MICs and zone diameters, Version 6.0. European Committee on Antimicrobial Susceptibility Testing (EUCAST); [cited 2016-01-01]. Avaiablefrom: http://www.eucast.org.
Fachini-Queiroz, F. C., Kummer, R., Estevão-Silva, C. F., Barros Carvalho, M. D., Cunha, M. J., Grespan, R., Bersani-Amado, C. A., Cuman, R. K. N. (2012) Effects of Thymol and Carvacrol, Constituents of Thymus vulgaris L. Essential Oil, on the Inflammatory Response. J Evid Based Complementary Altern Med. vol. 2012, Article ID 657026, 10 pages.
Holley, R. A., Patel, D. (2005) Improvement of shelflife and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol. 22, 273–292.
Kacar, O., Göksu, E., Azkan, N. (2006) The effect of different plant densites on some agronomic and quality characteristics of Oregano (Origanum onites L.) The Journal of Agricultural Faculty of Uludag Universit. 2, 51–60.
Kan, Y., Uçan, U. S., Kartal, M., Altun, M. L., Aslan, S., Sayar, E., Ceyhan, T. (2006) GC-MS analysis and antibacterial activity of cultivated Satureja cuneifolia Ten essential oil. Turkish Journal of Chemistr. 30, 253–259.
Kintzios, S. E. (2002) Profile of thyme multifaceted prince of the herbs. In: Kitzios, S. E. (ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis, London, 3–8.
Kirimer, N., Başer, K. H. C., Tümen, G. (1995) Carvacrol-rich plants in Turkey. Chem Nat Compd. 31, 37–41.
Kordali, S., Cakir, A., Ozer, H., Cakmakci, R., Kesdek, M., Mete, E. (2008) Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technol. 99, 8788–8795.
Mith, H., Dure, R., Delcenserie, V., Zhiri, A., Daube, G., Clinquart, A. (2014) Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria. Food Sci Nutr. 2, 403–416.
Şahin, N. F., Güllüce, M., Dafererad, D., Sökmen, A., Sökmen, M., Polissioud, M., Agar, G., Özer, H. (2004) Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Contro. 15, 549–557.
Sarac, N., Ugur, A. (2008) Antimicrobial activities of the essential oils of Origanum onites L., Origanum vulgare L. subspecies hirtum (link) letswaart, Satureja thymbra L., and Thymus cilicicus Boiss. & Bal. growing wild in Turkey. J Med Food. 11, 568–573.
Sarikurkcu, C., Zengin, G., Oskay, M., Uysal, S., Ceylan, R., Aktumsek, R. (2015) Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Ind. Crops Prod. 70, 178–184.
Schulz, H., Özkan, G., Baranska, M., Krüger, H., Özcan, M. (2005) Characterization of essential oil plants from Turkey by IR and Raman spectroscopy. Vibrational Spectroscop. 39, 249–256.
Sertkaya, E., Kaya, K. (2010) Acaricidal activities of the essential oils from several medicinal plants against the carmine spider mite (Tetranychus cinnabarinus Boisd.) (Acarina: Tetranychidae). Ind Crops Prod. 31, 107–112.
Skandamis, P., Tsigarida, E., Nychas, G. J. E. (2002) The effect of oregano essential oil on survival/ death of Salmonella typhimurium in meat stored at 50 °C under aerobic, VP/MAP conditions. Food Microbiol. 19, 97–103.
Solgi, M., Kafi, M., Taghavi, T., Naderi, R. (2009) Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers. Postharvest Biol. Technol. 53, 155–158.
Solórzano-Santos, F., Miranda-Novales, M. G. (2012) Essential oils from aromatic herbs as antimicrobial agents. Curr. Opin Biotechnol. 23, 136–141.
Tozlu, E., Cakir, A., Kordali, S., Tozlu, G., Ozer, H., Akcin, T. A. (2011) Chemical compositions and insecticidal effects of essential oils isolated from Achillea gypsicola, Satureja hortensis, Origanum acutidens and Hypericum scabrum against broadbean weevil (Bruchus dentipes). Sci. Horticult. 130, 9–17.
Vázquez, B. I., Fente, C., Franco, C. M., Vázquez, M. J., Cepeda, A. (2001) Inhibitory effects of eugenol and thymol on Penicillium citrinum strains in culture media and cheese. Int. J. Food Microbiol. 67, 157–163.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Kaskatepe, B., Yildiz, S.S., Kiymaci, M.E. et al. Chemical Composition and Antimicrobial Activity of the Commercial Origanum onites L. Oil Against Nosocomial Carbapenem Resistant Extended Spectrum Beta Lactamase Producer Escherichia coli Isolates. BIOLOGIA FUTURA 68, 466–476 (2017). https://doi.org/10.1556/018.68.2017.4.11
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/018.68.2017.4.11