Abstract
The aim of the present study is to extend the applicability of MRI measurements similar to those used in human diagnostics to the examination of water barriers in living plants, thus broadening their use in natural sciences. The cucumber, Cucumis sativus, and Phillyrea angustifolia, or false olive, were chosen as test plants. The MRI measurements were carried out on three samples of each plant in the same position vis-a-vis the MRI apparatus using a Siemens Avanto MRI scanner. Two different relaxation times were employed, T1 capable of histological mapping, and T2, used for the examination of water content. In the course of the analysis, it was found that certain histological formations and branching cause modifications to the intensity detected with relaxation time T2. Furthermore, these positions can also be found in T1 measurements. A. monotonie correlation (cucumber: p = 0.829; false olive: p = -0.84) was observed between the T1 and T2 measurements. In the course of the statistical analysis of the signal intensities of the xylems it was concluded that they cannot be regarded as independent in a statistical sense; these changes rather depend on the anatomie structure of the plant, as the intensity profile is modified by nodes, leaves and branches. This serves as a demonstration of the applicability of MRI to the measurement of well know plant physiological processes. The special parametrization required for this equipment, which is usually used in human diagnostics, is also documented in the present study.
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References
Abbott, J. A. (1999) Quality measurement of fruits and vegetables. Postharvest Biol. Technol. 15, 207–225.
Adriaensen, H., Musse, M., Quellec, S., Vignaud, A., Cambert, M., Mariette, F. (2013) MSE-MRI sequence optimisation for measurement of bi- and tri-exponential T2 relaxation in a phantom and fruit. Magn. Reson. Imaging 31, 1677–1689.
Andaur, J. E., Guesalaga, A. R., Agosin, E. E., Guarini, M. W., Irarrazaval, R. (2004) Magnetic Resonance Imaging for nondestructive analysis of wine grapes. J. Agric. Food Chem. 52, 165–170.
Bartholy, J., Pongrácz, R., Gelybó, Gy., Szabó, P. (2008) Analysis of expected climate change in the Carpathian Basin using the PRUDENCE results. Idöjárás 112, 249–265.
Berényi, E., Bogner, P., Horvath, Gy, Repa, I. (1997) Radiológia. Budapest, Springer Hungarica Kiadó Kft. (in Hungarian)
Borisjuk, L., Rolletschek, H., Neuberger, T. (2012) Surveying the plant’s world by magnetic resonance imaging. Plant 70, 129–146.
Borisjuk, L., Rolletschek, H., Neuberger, T. (2013) Nuclear magnetic resonance imaging of lipid in living plants. Prog. Lipid Res. 52, 465–187.
Bürggemann, N., Gessier, A., Kayler, Z., Keel, S. G., Badeck, F., Barthel, M., Buchmann, N., Brugnoli, E., Esperschütz, J., Gavrichkova, O., Ghashghaie, J., Gomez-Casanovas, N., Keitel, C., Knohl, A.. Kuptz, D., Palacio, S., Salmon, Y., Uchida, Y., Bahn, M. (2011) Carbon allocation and carbon isotope fluxes in the plant-soil-atmosphere continuüm: a review. Biogeosciences 8, 3457–3489.
Buxton, B. R. (2009) Introduction to Functional Magnetic Resonance Imaging. Cambridge Univer-sity Press.
Chen, R., McCarthy, M. J., Kim, S. M., Zion, B., Trans, A. (1996) Development of a high speed NMR technique for sensing maturity of avocados. Trans. ASAE 39, 2205–2209.
Cleveland, W. S., Devlin, S. J. (1988) Locally weighted regression: An approach to regression analysis by local fitting. J. Am. Stat. Assoc. 83, 596–610.
Cosgrove, D. J. (2005) Growth of the plant cell wall. Nat. Rev. Mol. Cell Biol. 6, 850–861.
Daudet, F. A., Lacointe, A., Gaudillère, J. R., Cruiziat, P. (2002) Generalized Münch coupling between sugar and water fluxes for modeling carbon allocation as affected by water status. J. Theor. Biol. 214, 481–198.
Edzes, H. T., Van Dusschoten, D., Van As, H. (1998) Quanitative T2 imaging of plant tissues by mean of multi-echo MRI microscopy. Magn. Reson. Imaging 16, 185–196.
Földes, T., Bogner, R., Zavoda, F., Repa, I. (2003) Opportunities of CT and MRI measurements in carbohydrate research. Magy. Radiológia 10, 231–237. (in Hungarian)
Guichard, S., Gary, C., Leonardi, C., Bertin, N. (2005) Analysis of growth and water relation of tomato fruits in relation to air vapor pressure deficit and plant fruit load. J. Plant Growth Regul. 24, 1–13.
Haraszty, A., Hortobagyi, T., Fridvalszky, L., Kiss, I., Pólya, L. (1978) Növényszervezettan ésnövény-élettan. Nemzeti Tankönyvkiadó. Budapest (in Hungarian)
Hills, B. P., Clark, C. J. (2003) Quality assessment of horticultural products by NMR. Ann. R. Nmr. S. 50, 75–120.
IPCC (2013) Summary for Policymakers. In: Stocker, T. E., D. Qin, G.-K. (eds) Climate Change 2013: The Physical Science Basis. Contribution of Working Group I. to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Plattner, M., Tignor, S. K., Allen, J., Boschung, A., Nauels, Y., Xia, V., Bex, R. M. Midgley (eds)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA. www.ipcc.ch
Ishida, N., Kobayashi, T., Koizumi, M., Kano, H. (1989) 1-H-NMR imaging of tomato fruits. Agric. Biol. Chem. 53, 2363–2367.
Jakusch, P., Anda, A. (2010) New possibilities in following the transport of water in living plants. lOth Annual Meeting of the European Meteorological Society (EMS) and 8th ECAC, Switzerland.
Joyce, D. C., Hockings, P. D., Mazucco, R. A., Shorter, A. J., Brereton, I. M. (1993) Heat treatment injury of mango fruit revealed by nondestructive magnetic resonance imaging. PostharvestBiol. Tech 3, 305–311.
Kenouche, S., Perrier, M., Bertin, N. J., Larionova, J., Ayadi, A., Zanca, M., Long, J., Bezzi, N., Stein, P. C., Guari, Y., Cieslak, M., Godin, C., Goze-Bac, C. (2014) In vivo quantitative NMR imaging of fruit tissues during growth using Spoiled Gradiënt Echo sequence. Magn. Reson. Imaging 32, 1418–1427.
Kovacs, K., Kuzmann, E., Vertes, A., Lévai, L., Cseh, E., Fodor, F. (2010) Effect of cadmium on iron uptake in cucumber roots: A. Mössbauer-spectroscopic study Plant Soil 327, 49–56.
Kuchenbrod, E., Kahler, E., Thürmer, F., Deichmann, R., Zimmermann, U., Haase, A. (1998) Functional Magnetic Resonance Imaging in intact plants-quantitative observation of flow in plant vessels. Magn. Reson. Imaging 16, 331–338.
Larcher, W. (2004) Physiological Plant Ecology. Springer-Verlag, Berlin Heidelberg.
Mazhar, M., Joyce, D., Cowinc, G., Breretonc, I., Hofmand, R., Collins, R., Gupta, M. (2015) Non-destructive ’H-MRI assessment of flesh bruising in avocado (Persea americana M.) cv. Hass. PostharvestBiol. Tech. 100, 33–10.
Mohr, H., Schopfer, R. (1995) Plant Physiology. Springer-Verlag. Berlin-Heidelberg.
Moreda, G. R., Ortiz. Canavate, J., Garcia-Ramos, F. J., Ruiz-Altisent, M. (2009) Non-destructive technologies for fruit and vegetable size determination - a review. J. Food Eng. 92, 119–136.
Muja, N., Bulte, J. W. M. (2009) Magnetic resonance imaging of cells in experimental disease models. Prog. Nucl. Mag. Res. Sp. 55, 61–77.
Musse, M., De Guio, E., Quellec, S., Cambert, M., Challois, S., Davenel, A. (2010) Quantification of microporosity in fruit by MRI at various magnetic fields: comparison with X-ray microtomography. Magn. Reson. Imaging 28, 1525–1534.
Pearcy, R. W., Ehleringer, J., Mooney, H. A., Rundel, R. W. (1991) Plant Physiological Ecology. Chapman and Hall, London-New York-Tokyo.
Peuke, A. D., Gessier, A., Trumbore, S., Windt, C. W., Homan, N., Gerkema, E., Van As, H. (2014) Phloem flow and sugar transport in Ricinus communis L. is inhibited under anoxic conditions of shoot or roots. Plant Cell Environ, DOI: 10.1111/pce.l2399
Prista, G. O., Agostinho, R. J., Cachao, M., A. (2015) Observing the past to better understand the future: a synthesis of the Neogene climate in Europe and its perspectives on present climate change. Open Geosci. 7, 65–83.
Raffo, A., Gainferri, R., Barbieri, R., Brosio, E. (2005) Rippening of banana fruit monitored by water relaxation and diffusion H-l-NMR measurements. Food Chem. 89, 149–158.
Saltveit, M. E. (1991) Determining tomato fruit maturity with nondestructive in vivo nuclear magnetic resonance imaging. Postharvest Biol. Tech. 1, 153–159.
Sanches, J., Biscegli, C. I., Durigan, J. F., Sim es, M. L., da Silva, W. T. L. (2003) Diagnosis of mechanical injuries in avocados by magnetic resonance imaging. In: V. World Avocado Congress, Granada, Malaga, Spain, pp. 695–700.
Schaafsma, T. J., Van As, H., Palstra, W. D., Snaar, J. E., de Jager, R. A. (1992) Quantitative measurement and imaging of transport processes in plants and porous media by 1H NMR. Magn. Reson. Imaging 10, 827–836.
Scheenen, T., Heemskerk, A., Jager, de A., Vergeldt, F., Van As, H. (2002) Functional imaging of plants: A. nuclear magnetic resonance study of cucumber plant. Biophys. J. 82, 481–192.
Van As, H., Scheenen, T., Vergeldt, F. J. (2009) MRI of intact plants. Photosynth. Res. 102, 213–222.
Van As, H. (2007) Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long-distance water transport. J. Exp. Bot. 58, 743–756.
Van As, H., Schaafsma, T. J. (1984) Noninvasive measurement of plant water flow by nuclear magnetic resonance. Biophys. J. 45, 469–172.
Van As, H., Reijnders, J. E., de Jager, R. A., R. A. van de Sanden, Schaafsma, T. J. (1994) In situ plant water balance studies using a portable NMR spectrometer. J. Exp. Bot. 45, 61–67.
Van As, H., Windt, C. W. (2008) Magnetic Resonance Imaging of Plants: Water Balance and Water Transport in Relation to Photosynthetic Activity. In: Aartsma, T. J., Matysik, J. (eds) Biophysical Techniques in Photosynthesis II. Springer, BerlinHeidelberg, pp. 55–75.
Van As, H., van Duynhoven, J. (2013) MRI of plants and foods. J. Magn. Reson. 229, 25–34.
Van der Weerd, L., Claessens, M. M. A. E., Edfé, C., Van As, H. (2002) Nuclear magnetic resonance imaging of membrane permeabilty changes in plants during osmotis stress. Plant Cell Environ. 25, 1539–1549.
Van der Weerd, L., Claessens, M. M. A. E., Ruttink, T., Vergeldt, F. J., Schaafsma, T. J., Van As, H. (2001) Quantitative NMR microscopy of osmotic stress responses in maize and pearl millet. J. Exp. Bot. 52/365, 2333–2343.
Verslues, R. E., Agarwal, M., Katiyar-Agarwal, S. Zhu, J. H., Zhu, J. K. (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant. J. 45, 523–539.
Wang, S. Y., Wang, R. C., Faust, M. (1988) Non-destructive detection of water-core in apple with nuclear magnetic resonance imaging. Hort-Science 35, 227–234.
Westbrook, C., Roth, C. K., Talbot, J. (2005) MRI in practice. Blackwell publishing. Italy.
Yang, M., Song, Y., Zhu, N., Zhao, Y., Liu, Y., Jiang, L. (2013) Dynamic measurements of C02 flow in water saturated porous medium at low temperature using MRI. Energy Proced. 37, 1267–1274.
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Jakusch, P., Kocsis, T., Székely, I.K. et al. The Application of Magnetic Resonance Imaging (Mri) to the Examination of Plant Tissues and Water Barriers. BIOLOGIA FUTURA 69, 423–436 (2018). https://doi.org/10.1556/018.69.2018.4.5
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DOI: https://doi.org/10.1556/018.69.2018.4.5