Abstract
Phenotypic and genotypic evaluation of wheat genetic resources and development of segregating populations are pre-requisites for identifying rust resistance genes. The objectives of this study were to assess adult plant resistance (APR) of selected wheat genotypes to leaf rust and stem rust and to develop segregating populations for resistance breeding. Eight selected Kenyan cultivars with known resistance to stem rust, together with local checks were evaluated for leaf rust and stem rust resistance at seedling stage and also across several environments. Selected diagnostic markers were used to determine the presence of known genes. All eight cultivars were crossed with local checks using a bi-parental mating design. Seedling tests revealed that parents exhibited differential infection types against wheat rust races. Cultivars Paka and Popo consistently showed resistant infection types at seedling stage, while Gem, Romany, Pasa, Fahari, Kudu, Ngiri and Kariega varied for resistant and susceptible infection types depending on the pathogen race used. The control cultivars Morocco and McNair consistently showed susceptible infection types as expected. In the field, all cultivars except for Morocco showed moderate to high levels of resistance, indicating the presence of effective resistance genes. Using diagnostic markers, presence of Lr34 was confirmed in Gem, Fahari, Kudu, Ngiri and Kariega, while Sr2 was present in Gem, Romany, Paka and Kudu. Seedling resistance gene, Sr35, was only detected in cultivar Popo. Overall, the study developed 909 F6:8 recombinant inbred lines (RILs) as part of the nested mating design and are useful genetic resources for further studies and for mapping wheat rust resistance genes.
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Acknowledgements
The authors thank the technical staff of the Agricultural Research Council-Small Grain Institute in South Africa for their assistance during field trials and data collection. The Agricultural Research Council, Winter Cereal Trust and the National Research Foundation of South Africa are highly acknowledged for funding this study. The authors have no conflict of interest to declare.
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Figlan, S., Baloyi, T.A., Hlongoane, T. et al. Adult Plant Resistance of Selected Kenyan Wheat Cultivars to Leaf Rust and Stem Rust Diseases. CEREAL RESEARCH COMMUNICATIONS 45, 68–82 (2017). https://doi.org/10.1556/0806.44.2016.052
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DOI: https://doi.org/10.1556/0806.44.2016.052