Abstract
The possibility of eye exposure for workers participating in manufacturing of nanoparticles or consumers using products containing nanoparticles has been reported, but toxicity studies on the eye are scarce. In this study, cytotoxicity of five nanoparticles including silver, ceria, silica, titanium and zinc were tested using Statens Seruminstitut Rabbit Cornea (SIRC) cells. When cells were treated with nanoparticles with concentrations of 1–100 μg/mL for 24 hr, zinc oxide nanoparticles showed higher toxicity to cornea cells. LC50 of zinc oxide nanoparticles was less than 25 μg/mL but those of other nanoparticles could not be calculated in this test, which means more than 100 μg/mL. Generation of reactive oxygen species was observed, and expression of apoptosis related biomarkers including Bax and Bcl-2 were changed after treatment of zinc oxide nanoparticles, while no other significant toxicity-related changes were observed in cornea cells treated with Ag, CeO2, SiO2 and TiO2 nanoparticles.
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References
Warheit, D.B. (2018) Hazard and risk assessment strategies for nanoparticle exposures: how far have we come in the past 10 years?. F1000Res., 7, 376.
Jeevanandan, J., Barhoum, A., Chan, Y.S., Dufresne, A. and Danquah, M.K. (2018) Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein. J. Nanotechnol., 9, 1050–1074.
Dwivedi, S., Saquib, Q., Ahmad, B., Ansari, S.M., Azam, A. and Musarrat, J. (2018) Toxicogenomics: A New Paradigm for Nanotoxicity Evaluation. Adv. Exp. Med. Biol., 1048, 143–161.
Kovalishyn, V., Abramenko, N., Kopernyk, I., Charochkina, L., Metelytsia, L., Tetko, I.V., Peijnenburg, W. and Kustov, L. (2018) Modelling the toxicity of a large set of metal and metal oxide nanoparticles using the OCHEM platform. Food Chem. Toxicol., 112, 507–517.
Choi, S.J. and Choy, J.H. (2014) Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction. Int. J.Nanomedicine, 2, 261–269.
Huang, Y.W., Cambre, M. and Lee, H.J. (2017) The toxicity of nanoparticles depends on multiple molecular and physicochemical mechanisms. Int. J. Mol. Sci., 18, 2702.
Mehra, N.K., Cai, D., Kuo, L., Hein, T. and Palakurthi, S. (2016) Safety and toxicity of nanomaterials for ocular drug delivery applications. Nanotoxicology, 10, 836–860.
Prow, T.W. (2010) Toxicity of nanomaterials to the eye. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol., 2, 317–333.
Cai, X., Seal, S. and McGinnis, J.F. (2016) Non-toxic retention of nanoceria in murine eyes. Mol. Vis., 22, 1176–1187.
Park, J.H., Jeong, H., Hong, J., Chang, M., Kim, M., Chuck, R.S., Lee, J.K. and Park, C.Y. (2016) The effect of silica nanoparticles on human corneal epithelial cells. Sci. Rep., 6, 37762.
Erika, S., Patrik, B., Tommy, C., Hodan, A., Fredrik, J. and Ulrica, E.J. (2014) Silver and gold nanoparticles exposure to in vitro cultured retina - studies on nanoparticle internalization, apoptosis, oxidative stress, glial- and microglial activity. PLoS ONE, 9, e105359.
Kim, J.H., Kim, J.H., Kim, K.W., Kim, M.H. and Yu, Y.S. (2009) Intravenously administered gold nanoparticles pass through the blood-retinal barrier depending on the particle size, and induce no retinal toxicity. Nanotechnology, 20, 505101.
Kim, J.S., Song, K.S., Sung, J.H., Ryu, H.R., Choi, B.G., Cho, H.S., Lee, J.K. and Yu, I.J. (2013) Genotoxicity, acute oral and dermal toxicity, eye and dermal irritation and corrosion and skin sensitisation evaluation of silver nanoparticles. Nanotoxicology, 7, 953–960.
An, W., Zhang, Y., Zhang, X., Li, K., Kang, Y., Akhtar, S., Sha, X. and Gao, L. (2018) Ocular toxicity of reduced graphene oxide or graphene oxide exposure in mouse eyes. Exp. Eye Res., 174, 59–69.
Guo, D., Bi, H., Wu, Q., Wang, D. and Cui, Y. (2013) Zinc oxide nanoparticles induce rat retinal ganglion cell damage through bcl-2, caspase-9 and caspase-12 pathways. J. Nanosci. Nanotechnol., 13, 3769–3777.
Guo, D., Bi, H., Liu, B., Wu, Q., Wang, D. and Cui, Y. (2013) Reactive oxygen species-induced cytotoxic effects of zinc oxidenanoparticles in rat retinal ganglion cells. Toxicol. In Vitro, 27, 731–738.
Kolle, S.N., Sauer, U.G., Moreno, M.C., Teubner, W., Wohlleben, W. and Landsiedel, R. (2016) Eye irritation testing of nanomaterials using the EpiOcularTM eye irritation test and the bovine corneal opacity and permeability assay. Part. Fibre Toxicol., 13, 18.
Kuo, T.R., Lee, C.F., Lin, S.J., Dong, C.Y., Chen, C.C. and Tan, H.Y. (2011) Studies of intracorneal distribution and cytotoxicity of quantum dots: riskassessment of eye exposure. Chem. Res. Toxicol., 24, 253–261.
Chen, C., Bu, W., Ding, H., Li, Q., Wang, D., Bi, H. and Guo, D. (2017) Cytotoxic effect of zinc oxide nanoparticles on murine photoreceptor cellsvia potassium channel block and Na+ /K+ -ATPase inhibition. Cell Prolif., 50, e12339.
Wang, L., Chen, C., Guo, L., Li, Q., Ding, H., Bi, H. and Guo, D. (2018) Zinc oxide nanoparticles induce murine photoreceptor cell death viamitochondria-related signaling pathway. Artif. Cells Nanomed. Biotechnol., 28, 1–12.
Guo, D.D., Li, Q.N., Li, C.M. and Bi, H.S. (2015) Zinc oxide nanoparticles inhibit murine photoreceptor-derived cell proliferation and migration via reducing TGF-β and MMP-9 expression in vitro. Cell Prolif., 48, 198–208.
Lee, H., Park, J. and Park, K. (2017) Cytotoxicity of nanoparticles in cultured THP-1 cells under the standardized condition of size distribution. Yakhak Hoeji, 61, 109–116.
Lee, H., Park, J. and Park, K. (2017) Size distribution of silica dioxide nanoparticles after calibrated sonication and cytotoxicity in cultured A549 cells. Yakhak Hoeji, 61, 309–316.
Ripps, H. and Chappell, R.L. (2014) Review: zinc’s functional significance in the vertebrate retina. Mol. Vis., 20, 1067–1074.
Ugarte, M., Osborne, N.N., Brown, L.A. and Bishop, P.N. (2013) Iron, zinc, and copper in retinal physiology and disease. Surv. Ophthalmol., 58, 585–609.
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Lee, H., Park, K. In Vitro Cytotoxicity of Zinc Oxide Nanoparticles in Cultured Statens Seruminstitut Rabbit Cornea Cells. Toxicol Res. 35, 287–294 (2019). https://doi.org/10.5487/TR.2019.35.3.287
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DOI: https://doi.org/10.5487/TR.2019.35.3.287