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Fig. 1 | Diabetologia

Fig. 1

From: The role of lipid peroxidation products and oxidative stress in activation of the canonical wingless-type MMTV integration site (WNT) pathway in a rat model of diabetic retinopathy

Fig. 1

Effects of 7-ketocholesterol, H2O2 and HNE on cell viability and TOPFLASH activity. a ARPE19 cells were treated with various concentrations of 7-ketocholesterol (7-KC), (b) H2O2 and (c) HNE for 16 h. Cell viability was determined using a commercial kit and expressed as per cent of control cells. Values are mean ± SD, n = 3; *p < 0.05 and **p < 0.01. d ARPE19 cells were transfected for 8 h with the TOPFLASH or FOPFLASH plasmid and then treated with various concentrations of 7-ketocholesterol, (e) H2O2 and (f) HNE for another 16 h. TOPFLASH activity (white bars) and FOPFLASH activity (grey bars) were measured by luminometer and normalised to Renilla luciferase activity. Values are mean ± SD, n = 3; *p < 0.05 and **p < 0.01

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