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Fig. 5 | Diabetologia

Fig. 5

From: The role of lipid peroxidation products and oxidative stress in activation of the canonical wingless-type MMTV integration site (WNT) pathway in a rat model of diabetic retinopathy

Fig. 5

CTGF was induced by HNE and attenuated by NAC. a ARPE19 cells were pretreated for 1 h with NAC (1 mmol/l) prior to challenge with HNE (10 μmol/l) for 6 h. CTGF levels were determined by western blot analysis using 50 μg of total proteins from each sample. CTGF was quantified (b) by densitometry from three independent experiments, normalised to β-actin levels and expressed as per cent of levels in control cells. Values mean ± SD; n = 3; **p < 0.01 for HNE compared with control); †† p < 0.01 for HNE+NAC compared with HNE. c ARPE19 cells were transfected for 8 h with the TOPFLASH or FOPFLASH plasmid and then pretreated with IgG or 2F1 (20 μg/ml) for 1 h prior to incubation with HNE (10 μmol/l) for another 16 h. TOPFLASH activity (white bars) and FOPFLASH activity (grey bars) were measured by luminometer and normalised to Renilla luciferase activity. Values are mean ± SD; n = 3; **p < 0.01 for HNE compared with control; †† p < 0.01 for HNE+2F1 compared with HNE. d ARPE19 cells were pretreated for 1 h with IgG or 2F1 (20 μg/ml) prior to incubation with HNE (10 μmol/l) for 6 h. CTGF levels were determined by western blot analysis using 50 μg of total proteins from each sample. CTGF was quantified (e) by densitometry from three independent experiments, normalised to β-actin levels and expressed as per cent of levels in control cells. Values are mean ± SD; **p < 0.01 for HNE compared with control; †† p < 0.01 for HNE+2F1 compared with HNE

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