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Fig. 6 | Diabetologia

Fig. 6

From: The role of lipid peroxidation products and oxidative stress in activation of the canonical wingless-type MMTV integration site (WNT) pathway in a rat model of diabetic retinopathy

Fig. 6

β-Catenin levels were increased by HNE and HOG-LDL, and decreased by NAC in BRCEC. a BRCEC were pretreated for 1 h with NAC (1 mmol/l) prior to challenge with HNE (10 μmol/l). Cytosolic β-catenin was measured by western blot analysis using 50 μg of proteins from each sample, normalised to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels and quantified in bar graph as per cent of levels in control. Values are mean ± SD; n = 3; **p < 0.01 for HNE compared with control; †† p < 0.01 for HNE+NAC compared with HNE. b BRCEC were treated with normal LDL (N-LDL) and HOG-LDL (20 μg/ml) for 12 h. β-Catenin levels were determined by western blot analysis using 50 μg of proteins from each sample, normalised to β-actin levels and expressed as per cent of those in control. Values are mean ± SD; n = 3; **p < 0.01 for HOG-LDL compared with N-LDL; † p < 0.05 for HOG+NAC compared with HOG-LDL

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