Fig. 7

Effects of oxidative stress, lipid peroxidation products and NAC on the WNT pathway in the retina of diabetic rats. a Normal rats at 8 weeks of age received an intraperitoneal injection of streptozotocin (50 mg/kg in 10 mmol/l of citrate buffer; pH 4.5) after an overnight fast. Age-matched control rats received an injection of the citrate buffer alone for non-diabetic control (Non-DM). NAC (1.4 g/kg) was administered to diabetic groups (DM) in the drinking water for 8 weeks. The ocular sections were immunostained separately with antibodies for 3-nitrotyrosine, HNE and β-catenin using DAB staining. GCL, Ganglion cell layer; OPL, outer plexiform layer. b The eyecups were dissected, and levels of total LRP6, β-catenin and CTGF were determined by western blot analysis using 50 μg of total proteins from each sample. Each lane represents an individual animal. Wt, wild-type