Abstract
To test whether amino acid mutations in the PBC and PHI loops of VP2 are involved in the replication and virulence of infectious bursal disease virus (IBDV), a pair of viruses, namely the moderately virulent IBDV (rGx-F9VP2) and the attenuated strain (rGt), were used. Residue mutations A222P (PBC) and S330R (PHI), selected by sequence comparison, were introduced individually into rGx-F9VP2 by using a reverse genetics system. In addition, the reverse mutation of either P222A or R330S was introduced into rGt. The four modified viruses were then rescued and evaluated in vitro (CEF cells) and in vivo (SPF chickens). Results showed that A222P elevated the replication efficiency of rGx-F9VP2 while P222A reduced that of rGt in CEF cells. A mutation at residue 330 did not alter IBDV replication. In addition, animal experiments showed that a single mutation at either residue 222 or 330 did not significantly influence the virulence of IBDV. In conclusion, residue 222 in PBC of VP2 is involved in the replication efficiency of IBDV in vitro but does not affect its virulence in vivo, further facilitating our understanding of the gene-function of IBDV.
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References
Birghan, C., Mundt, E., and Gorbalenya, A.E. (2000). A non-canonical Lon proteinase lacking the ATPase domain employs the Ser-Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virus. Embo J 19, 114–123.
Boot, H.J., Ter Huurne, A., Hoekman, A.J.W., Peeters, B.P.H., and Gielkens, A.L.J. (2000). Rescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulent phenotype. J Virol 74, 6701–6711.
Brandt, M., Yao, K., Liu, M.H., Heckert, R.A., and Vakharia, V.N. (2001). Molecular determinants of virulence, cell tropism, and pathogenic phenotype of infectious bursal disease virus. J Virol 75, 11974–11982.
Cosgrove, A.S. (1962). An apparently new disease of chickens:avian nephrosts. Avian Dis 6, 385–389.
Coulibaly, F., Chevalier, C., Gutsche, I., Pous, J., Navaza, J., Bressanelli, S., Delmas, B., and Rey, F.A. (2005). The birnavirus crystal structure reveals structural relationships among icosahedral viruses. Cell 120, 761–772.
Da Costa, B., Chevalier, C., Henry, C., Huet, J.C., Petit, S., Lepault, J., Boot, H., and Delmas, B. (2002). The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2. J Virol 76, 2393–2402.
Durairaj, V., Sellers, H.S., Linnemann, E.G., Icard, A.H., and Mundt, E. (2011). Investigation of the antigenic evolution of field isolates using the reverse genetics system of infectious bursal disease virus (IBDV). Arch Virol 156, 1717–1728.
Garriga, D., Querol-Audi, J., Abaitua, F., Saugar, I., Pous, J., Verdaguer, N., Caston, J.R., and Rodriguez, J.F. (2006). The 2.6-angstrom structure of infectious Bursal disease virus-derived T=1 particles reveals new stabilizing elements of the virus capsid. J Virol 80, 6895–6905.
Jackwood, D.J., and Sommer-Wagner, S.E. (2011). Amino acids contributing to antigenic drift in the infectious bursal disease Birnavirus (IBDV). Virology 409, 33–37.
Jackwood, D.J., Sreedevi, B., LeFever, L.J., and Sommer-Wagner, S.E. (2008). Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity. Virology 377, 110–116.
Le Nouen, C., Rivallan, G., Toquin, D., Darlu, P., Morin, Y., Beven, W., de Boisseson, C., Cazaban, C., Comte, S., Gardin, Y., and Eterradossi, N. (2006). Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-B-reassorted isolate. J Gen Virol 87, 209–216.
Lee, C.C., Ko, T.P., Chou, C.C., Yoshimura, M., Doong, S.R., Wang, M.Y., and Wang, A.H.J. (2006). Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6 angstrom resolution: implications in virion assembly and immunogenicity. J Struct Biol 155, 74–86.
Letzel, T., Coulibaly, F., Rey, F.A., Delmas, B., Jagt, E., van Loon, A.A.M.W., and Mundt, E. (2007). Molecular and structural bases for the antigenicity of VP2 of infectious bursal disease virus. J Virol 81, 12827–12835.
Li, Z, Qi, X., Ren, X., Cui, L., Wang, X., and Zhu, P. (2015). The molecular characteristics and evolution analysis of a very virulent infectious bursal disease virus. Sci China Life Sci 58, 731–738.
Lim, B.L., Cao, Y.C., Yu, T., and Mo, C.W. (1999). Adaption of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2. J Virol 73, 2854–2862.
Liu, M.H., and Vakharia, V.N. (2004). VP1 protein of infectious bursal disease virus modulates the virulence in vivo. Virology 330, 62–73.
Lucio, B., and Hitchner, S. B. (1979). Infectious bursal disease emulsified vaccine: effect upon neutralizing antibody levels in the dam and subsequent protection of the progeny. Avian Dis 23, 466–478.
Muller, H., Islam, M.R., and Raue, R. (2003). Research on infectious bursal disease: the past, the present and the future. Vet Microbiol 97, 153–165.
Mundt, E. (1999). Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2. J Gen Virol 80, 2067–2076.
Mundt, E., Beyer, J., and Muller, H. (1995). Identification of a novel viral protein in infectious bursal disease virus infected cells. J Gen Virol 76, 437–443.
Qi, X., Gao, H., Gao, Y., Qin, L., Wang, Y., Gao, L., and Wang, X. (2009). Naturally occurring mutations at residues 253 and 284 in VP2 contribute to the cell tropism and virulence of very virulent infectious bursal disease virus. Antiviral Res 84, 225–233.
Qi, X., Gao, Y., Gao, H., Deng, X., Bu, Z., Wang, X., Fu, C., and Wang, X. (2007). An improved method for infectious bursal disease virus rescue using RNA polymerase II system. J Virol Methods 142, 81–88.
Qi, X., Zhang, L., Chen, Y., Gao, L., Wu, G., Qin, L., Wang, Y., Ren, X., Gao, Y., Gao, H., and Wang, X. (2013). Mutations of residues 249 and 256 in VP2 are involved in the replication and virulence of infectious bursal disease virus. PLoS One 2013, 8: e70982.
Raue, R., Islam, M.R., Islam, M.N., Islam, K.M., Badhy, S.C., Das, P.M., and Muller, H. (2004). Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickens. Avian Pathol 33, 181–189.
Rautenschlein, S., Yeh, H.Y., and Sharma, J.M. (2003). Comparative immunopathogenesis of mild, intermediate, and virulent strains of classic infectious bursal disease virus. Avian Dis 47, 66–78.
Reed, L.J., and Muench, H. (1938). A simple method of estimating fifty percent endpoints. Am J Hyg 27, 493–497.
Schroder, A., van Loon, A., Goovaerts, D., and Mundt, E. (2000). Chimeras in noncoding regions between serotypes I and II of segment A of infectious bursal disease virus are viable and show pathogenic phenotype in chickens. J Gen Virol 81, 533–540.
van Loon, A., de Haas, N., Zeyda, I., and Mundt, E. (2002). Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens. J Gen Virol 83, 121–129.
von Einem, U.I., Gorbalenya, A.E., Schirrmeier, H., Behrens, S.E., Letzel, T., and Mundt, E. (2004). VP1 of infectious bursal disease virus is an RNA-dependent RNA polymerase. J Gen Virol 85, 2221–2229.
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Qi, X., Gao, X., Lu, Z. et al. A single mutation in the PBC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus. Sci. China Life Sci. 59, 717–723 (2016). https://doi.org/10.1007/s11427-016-5054-1
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DOI: https://doi.org/10.1007/s11427-016-5054-1