Fig. 6

CM-hAMSC modulates the secretion of Th cytokines. The secretion of cytokines specific for the different T-cell subsets was evaluated each day during 6 days of co-culture in absence (white squares) or presence (black squares) of CM-hAMSC. The amount of cytokine produced by the CM-hAMSC itself was measured on day one only and is represented by a white diamond. The quantification of (A) Th1 cytokines (IFN-γ, TNFα, IL-1β, IL-2, IL-12p70), (B) Th2 cytokines (IL-4, IL-5, IL-6, IL-10, IL-13), (C) Th17 cytokines (IL-17A, IL-22), (D) IL-9 and sIL-2R were evaluated as described in Materials and Methods. Data represent the mean and standard error mean (SEM) of at least three experiments. Asterisks indicate statistically significant differences between MLR-CM-hAMSC and MLR; *p < 0.05; **p < 0.01