Fig. 1
From: Purkinje Cell-Specific Ablation of CaV2.1 Channels is Sufficient to Cause Cerebellar Ataxia in Mice
Generation of conditional Cacna1a Purkinje cell-specific knockout mice. a Schematic representation of the genomic structure of the relevant part of the floxed and recombined Cacna1a alleles. Black boxes indicate exons (E). Triangles indicate the position of loxP sites. Arrows indicate the position of the primers used for the RT-PCR. b RT-PCR of cerebellar RNA amplifies a 580-bp product from the non-recombined Cacna1a transcript and a 490-bp product from transcripts that lack exon 4. c–f Anti-CaV2.1-α1A immunostaining in cerebellar sections of P30 WT (c, e) and PCα1KO (d, f) mice. CN cerebellar nuclei, G granule cell layer, M molecular layer, PC Purkinje cell layer, WM white matter. Roman numbers indicate the cerebellar lobules