Fig. 6
From: Altered Extracellular Vesicle MicroRNA Expression in Ischemic Stroke and Small Vessel Disease
Circulating miR-17 family expression in naïve and post-tMCAO rats. Total serum expression of miR-17-5p (a), miR-20b-5p (b) and miR-93-5p (c) was assessed in naïve normotensive WKY and hypertensive SHRSP rats (n = 5–7/group). EV miRNA expression of miR-17-5p (d), miR-20b-5p (e) and miR-93-5p (f) was assessed in naïve WKY and SHRSP rats (n = 5–7/group). EV expression of miR-17-5p (g), miR-20b-5p (h) and miR-93-5p (i) was assessed in serum of SHRSP rats at 24 h following tMCAO (n = 3) or sham surgery (n = 3). Change in miRNA expression was assessed by qRT-PCR and RQ calculated from ΔΔCt following normalisation to a spike housekeeper miRNA, cel-miR-39, and compared to control (WKY or sham surgery) rats. Data are presented as RQ ± RQmax/RQmin. Statistical probability of differences in expression observed were calculated using unpaired two-tailed Student’s t test vs. control rats: *p < 0.05; ***p < 0.001