Fig. 7
From: Altered Extracellular Vesicle MicroRNA Expression in Ischemic Stroke and Small Vessel Disease
MiR-17 family expression in SHRSP brain or brain-derived EV post-tMCAO. miRNA expression was determined a, c, e peri-infarct tissue of SHRSP rats 24 h post-tMCAO or sham surgery (n = 6/group) or b, d, f brain-derived EV from peri-infarct tissue of SHRSP rats 24 h post-tMCAO or sham surgery (n = 3/group) for miR-17-5p (a, b); miR-20b-5p (c, d) or miR-93-5p (e, f). Change in miRNA expression was assessed by qRT-PCR and RQ calculated from ΔΔCt following normalisation to a spike in housekeeper miRNA, cel-miR-39, and compared to sham operated SHRSP rats. Data are presented as RQ ± RQmax/RQmin. Statistical probability of differences in expression observed were calculated using unpaired two-tailed Student’s t test with Bonferroni’s multiple comparisons test vs. sham-operated rats