Fig. 4
From: Adenine base editor corrected ADPKD point mutations in hiPSCs and kidney organoids
Detection of ADPKD related disease phenotypes in kidney organoids and renal epithelial cells. A The bright light image of ADPKD kidney organoids after addition of forskolin for 0, 3 and 7 days. Scale bar, 200 μm. B The bright light image of kidney organoids before and after adding forskolin 7 days. Images in each group are the same kidney organoid. The ADPKD group showed obvious cyst formation, while the H1 hESC and corrected grou Immunofluorescence staining p showed no cyst formation. Scale bar, 200 μm. C Area statistics of ADPKD kidney organoids after addition of forskolin for 7 days. Error bars indicated SD (n = 10). Statistical significance was determined using the two-tailed Student’s t test. ***p < 0.001. D Immunofluorescence staining of LTL in the ADPKD kidney organoid. Scale bar, 200 μm. E HE staining of ADPKD kidney organoids after addition of forskolin for 7 days. Scale bar, 250 μm. F RT-qPCR detected mitochondrial DNA r16S levels in kidney organoids. H1 hESC differentiated kidney organoids as a control. GAPDH as an internal reference. Error bars indicated SD (n = 3). Statistical significance was determined by the two-tailed Student’s t test. ***p < 0.001. G RT-qPCR detected mitochondrial DNA r16S levels in ADPKD hiPSCs. H1 hESC as a control. GAPDH as an internal reference. H Bright-field image of renal epithelial cells. Scale bar, 200 μm. I Immunofluorescence staining showed the expression of Ki67 protein in renal epithelial cells. H1 hESC differentiated renal epithelial cells were used as a control. Scale bar, 200 μm. J Statistical results of Ki67-positive cells. In each group n = 200. Error bars indicate SD. Statistical significance was determined using the two-tailed Student’s t test. ***p < 0.001